Tan0008795 (gene) Snake gourd v1

BLAST of Tan0008795 vs. ExPASy Swiss-Prot
Match: Q9LY87 (E3 ubiquitin-protein ligase RGLG2 OS=Arabidopsis thaliana OX=3702 GN=RGLG2 PE=1 SV=1)

HSP 1 Score: 568.9 bits (1465), Expect = 4.8e-161
Identity = 298/468 (63.68%), Postives = 359/468 (76.71%), Query Frame = 0

Query: 1   MGGKNSKDSGQRE-----FDSYGSGNSSTW-----------DHYNYPPPSPYVQ--QYSY 60
           MG  NSK++ ++        S  S +SS+W           + YNYPPP  Y Q  +Y+ 
Sbjct: 1   MGTGNSKENWRQSSFRSTSASSASPSSSSWASQQSYPQYGAESYNYPPPPSYAQPPEYTQ 60

Query: 61  PQPSDYGSQ--------TPQPQ-------RRLDRRYSRIADNYQSLDEVSAALAQAGLES 120
           P P  Y +Q         P  Q       +RL+R+YS+I+D+Y SL++V+ ALA+AGLES
Sbjct: 61  PPPPLYSTQPYSAPSYSAPPSQSYGSDNKKRLERKYSKISDDYSSLEQVTEALARAGLES 120

Query: 121 SNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQNPYEQAISIIGQTLSAFDEDNMIPCYG 180
           SNLIVGIDFTKSNEWTG+RSFNR+SLH IG+  NPYEQAI+IIG+TL+AFDEDN+IPCYG
Sbjct: 121 SNLIVGIDFTKSNEWTGARSFNRKSLHFIGSSPNPYEQAITIIGRTLAAFDEDNLIPCYG 180

Query: 181 FGDASTHDQDVFSFYDDDRICNGFEEVLHRYREIVPNLRLAGPTSFAPIIEMAMTIVEQT 240
           FGDASTHDQDVFSF  +DR CNGFEEVL RY+EIVP L+LAGPTSFAPII+MAMTIVEQ+
Sbjct: 181 FGDASTHDQDVFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQS 240

Query: 241 GGQYHVLLIIADGQVTRSVDTHNGQLSSQERKTVDAIVRASEYPLSIILVGVGDGPWDMM 300
           GGQYHVL+IIADGQVTRSVDT NGQLS QE+KTVDAIV+AS+ PLSI+LVGVGDGPWDMM
Sbjct: 241 GGQYHVLVIIADGQVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWDMM 300

Query: 301 KDFDDNIPARAFDNFQFVNFTEIMSKSVNSARRQTEFALSALMEIPSQYQATLELGLLGR 360
           ++FDDNIPARAFDNFQFVNFTEIM+K+   + ++TEFALSALMEIP QY+AT+EL LLGR
Sbjct: 301 REFDDNIPARAFDNFQFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIELNLLGR 360

Query: 361 QRGSSPERVPLPPPLYGATPSNGYLRTPRSNSFQTRTPSHSRYNQGVGTT---TSSSSHY 420
           + G  PER PLPPP+ G + S    +  R  SF+   P H      V ++     +SS  
Sbjct: 361 RNGYIPERFPLPPPMRGGSSSYNSPKPSRLPSFKPSVPPHPTEGYHVRSSPVPPPTSSAS 420

Query: 421 DNQVCPICLTNPKDMAFGCGHQTCCDCGETLELCPICRSFIQTRIRLY 433
           DNQ+CPICL+NPKDMAFGCGHQTCC+CG  L++CPICR+ IQTRI+LY
Sbjct: 421 DNQLCPICLSNPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKLY 468

BLAST of Tan0008795 vs. ExPASy Swiss-Prot
Match: Q8LB88 (E3 ubiquitin-protein ligase RGLG5 OS=Arabidopsis thaliana OX=3702 GN=RGLG5 PE=1 SV=1)

HSP 1 Score: 550.4 bits (1417), Expect = 1.8e-155
Identity = 289/447 (64.65%), Postives = 343/447 (76.73%), Query Frame = 0

Query: 1   MGGKNSKDSGQ-----REFDSYGSGN---SSTWDHYNYPPPSPYVQQYSYPQPS-----D 60
           MGG +SK+S +     R ++   SG+   SS WD       S Y Q  ++P  S     +
Sbjct: 1   MGGSSSKESPRGGGSGRRYERSVSGSSSYSSAWDQ------SSYYQTPNHPSASPVSSYN 60

Query: 61  YGSQTPQPQRRLDRRYSRIADNYQSLDEVSAALAQAGLESSNLIVGIDFTKSNEWTGSRS 120
            G QTP   + L+R+YSRIADNY+S+DEV+AAL+ AGLESSNLIVGID TKSNEWTG+RS
Sbjct: 61  SGRQTP---KNLERKYSRIADNYRSIDEVTAALSHAGLESSNLIVGIDVTKSNEWTGARS 120

Query: 121 FNRRSLHHIGNGQNPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQDVFSFYDDDRI 180
           F R+SLH IG   NPY+QAISIIG+TLS FDEDN+IPCYGFGDA+THDQDVFSF  +D  
Sbjct: 121 FGRKSLHFIGTTPNPYQQAISIIGKTLSVFDEDNLIPCYGFGDATTHDQDVFSFNPNDTY 180

Query: 181 CNGFEEVLHRYREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVD 240
           CNGFEEVL  YREIVP LRL+GPTSFAPIIE AMTIVE++GGQYHVLLIIADGQVTRSVD
Sbjct: 181 CNGFEEVLMCYREIVPQLRLSGPTSFAPIIERAMTIVEESGGQYHVLLIIADGQVTRSVD 240

Query: 241 THNGQLSSQERKTVDAIVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDNFQFVNF 300
           T NG  S QE++T+DAIVRASEYPLSI+LVGVGDGPWD M+ FDDNIPARAFDNFQFVNF
Sbjct: 241 TDNGGFSPQEQQTIDAIVRASEYPLSIVLVGVGDGPWDTMRQFDDNIPARAFDNFQFVNF 300

Query: 301 TEIMSKSVNSARRQTEFALSALMEIPSQYQATLELGLLGRQRGSSPERVPLPPPLYGATP 360
           T+IMSK+++ AR++ EFALSALMEIPSQY+ATLELGLLG++ G  P+R+ LPPP Y    
Sbjct: 301 TDIMSKNIDPARKEAEFALSALMEIPSQYKATLELGLLGQRTGHCPDRIALPPPTYATQS 360

Query: 361 SNGYLRTPRSNSFQTRTPSHSRYNQGVGTTTSSSSHYDN--QVCPICLTNPKDMAFGCGH 420
                RT RS SFQ +      Y+ GV +T  S++H ++  Q CP+CL + K+MAF CGH
Sbjct: 361 MRNSPRTSRSTSFQNKP-----YDNGVSSTPPSTTHNESQQQFCPVCLVSAKNMAFNCGH 420

Query: 421 QTCCDCGETLELCPICRSFIQTRIRLY 433
           QTC  CGE L +CPICRS I  RI+LY
Sbjct: 421 QTCAGCGEDLHVCPICRSSISVRIKLY 433

BLAST of Tan0008795 vs. ExPASy Swiss-Prot
Match: Q9SS90 (E3 ubiquitin-protein ligase RGLG1 OS=Arabidopsis thaliana OX=3702 GN=RGLG1 PE=1 SV=1)

HSP 1 Score: 541.2 bits (1393), Expect = 1.1e-152
Identity = 276/415 (66.51%), Postives = 331/415 (79.76%), Query Frame = 0

Query: 31  PPPSP---YVQQYS---YPQPSDYGSQTPQP-------QRRLDRRYSRIADNYQSLDEVS 90
           P PSP   Y  QYS   Y   +   +  P P       +++ DRRYS+I+DNY SL +VS
Sbjct: 85  PAPSPASSYGHQYSQEGYASAASQPNYPPPPSQSQVADRKKFDRRYSKISDNYSSLLQVS 144

Query: 91  AALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQNPYEQAISIIGQTLSAF 150
            AL +AGLESSNLIVGIDFTKSNEWTG++SFNR+SLHH+ N  NPYEQAI+IIG+TL+AF
Sbjct: 145 EALGRAGLESSNLIVGIDFTKSNEWTGAKSFNRKSLHHLSNTPNPYEQAITIIGRTLAAF 204

Query: 151 DEDNMIPCYGFGDASTHDQDVFSFYDDDRICNGFEEVLHRYREIVPNLRLAGPTSFAPII 210
           DEDN+IPCYGFGDASTHDQDVFSFY + R CNGFEEVL RYREIVP L+LAGPTSFAPII
Sbjct: 205 DEDNLIPCYGFGDASTHDQDVFSFYPEGRFCNGFEEVLARYREIVPQLKLAGPTSFAPII 264

Query: 211 EMAMTIVEQTGGQYHVLLIIADGQVTRSVDTHNGQLSSQERKTVDAIVRASEYPLSIILV 270
           EMAMT+VEQ+ GQYHVL+IIADGQVTRSVDT +G+LS QE+KTVDAIV+AS  PLSI+LV
Sbjct: 265 EMAMTVVEQSSGQYHVLVIIADGQVTRSVDTEHGRLSPQEQKTVDAIVKASTLPLSIVLV 324

Query: 271 GVGDGPWDMMKDFDDNIPARAFDNFQFVNFTEIMSKSVNSARRQTEFALSALMEIPSQYQ 330
           GVGDGPWDMM++FDDNIPARAFDNFQFVNFTEIMSK+ + +R++TEFALSALMEIP QY+
Sbjct: 325 GVGDGPWDMMQEFDDNIPARAFDNFQFVNFTEIMSKNKDQSRKETEFALSALMEIPPQYK 384

Query: 331 ATLELGLLGRQRGSSPERVPLPPPLYGATPSNGYLRTPRSNSFQTRTPSHSRYNQGVGTT 390
           AT+EL LLG + G+ P+R+PLPPP+     S     + R  +F+   P +   ++     
Sbjct: 385 ATIELNLLGVRNGNIPQRIPLPPPV----QSGSSFSSSRIPNFEPSVPPYPFESK----- 444

Query: 391 TSSSSHYDNQVCPICLTNPKDMAFGCGHQTCCDCGETLELCPICRSFIQTRIRLY 433
              SS  D Q+CPICL+NPK+MAFGCGHQTCC+CG  L++CPICR+ IQTRI+LY
Sbjct: 445 -QMSSADDIQLCPICLSNPKNMAFGCGHQTCCECGPDLKVCPICRAPIQTRIKLY 489

BLAST of Tan0008795 vs. ExPASy Swiss-Prot
Match: Q9SAL0 (E3 ubiquitin-protein ligase RGLG4 OS=Arabidopsis thaliana OX=3702 GN=RGLG4 PE=1 SV=1)

HSP 1 Score: 427.9 bits (1099), Expect = 1.3e-118
Identity = 221/416 (53.12%), Postives = 280/416 (67.31%), Query Frame = 0

Query: 17  YGSGNSSTWDHYNYPPPSPYVQQYSYPQPSDYGSQTPQPQRRLDRRYSRIADNYQSLDEV 76
           +GSG S +  +      S    Q   P PSD             ++Y+ I D + SLD+V
Sbjct: 10  FGSGKSRSSRNMTLGTTS---SQSHEPSPSDPSLSLADNTNATKKKYALIPDRFSSLDQV 69

Query: 77  SAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQNPYEQAISIIGQTLSA 136
           S AL +AGLESSNLI+G+DFTKSNEWTG  SF+ + LH +G   NPYE+AI +IGQTL+ 
Sbjct: 70  SKALREAGLESSNLILGVDFTKSNEWTGKTSFDGKCLHALGETSNPYEKAIFVIGQTLAP 129

Query: 137 FDEDNMIPCYGFGDASTHDQDVFSFYDDDRICNGFEEVLHRYREIVPNLRLAGPTSFAPI 196
           FDEDN+IPC+GFGD++THD++VF F+ D+  C+GFEEVL  Y+ I PNLRL+GPTS+ P+
Sbjct: 130 FDEDNLIPCFGFGDSTTHDEEVFGFHSDNSPCHGFEEVLACYKRIAPNLRLSGPTSYGPL 189

Query: 197 IEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTHNGQLSSQERKTVDAIVRASEYPLSIIL 256
           I+ A+ IVE+  GQ+HVL+I+ADGQVTR  D   G+LS QE+ T+DAIV AS Y LSI+L
Sbjct: 190 IDAAVDIVEKNNGQFHVLVIVADGQVTRGTDMAEGELSQQEKTTIDAIVNASSYALSIVL 249

Query: 257 VGVGDGPWDMMKDFDDNIPARAFDNFQFVNFTEIMSKSVNSARRQTEFALSALMEIPSQY 316
           VGVGDGPW+ M+ FDD IP R FDNFQFVNFTEIM+++   + ++T FAL+ALMEIP QY
Sbjct: 250 VGVGDGPWEDMRKFDDKIPKREFDNFQFVNFTEIMTRNSPESAKETAFALAALMEIPFQY 309

Query: 317 QATLELGLLGRQRGSSPERVPLPPPLYGATPSNGYLRTPRSNSFQTRTPSHSRYNQGVGT 376
           QA +EL LLG+Q G +   VP PPP+    P+N  L            PS          
Sbjct: 310 QAAIELRLLGKQTGLAKTIVPRPPPIPYTPPTNAEL------------PS---------- 369

Query: 377 TTSSSSHYDNQVCPICLTNPKDMAFGCGHQTCCDCGETLELCPICRSFIQTRIRLY 433
           T S +S    Q CPICLTN KD+AF CGH TC DCG  +  CPICR  I  R++LY
Sbjct: 370 TASPASPEQTQSCPICLTNRKDVAFSCGHMTCGDCGSKISNCPICRVRITNRLKLY 400

BLAST of Tan0008795 vs. ExPASy Swiss-Prot
Match: Q8RX26 (E3 ubiquitin-protein ligase RGLG3 OS=Arabidopsis thaliana OX=3702 GN=RGLG3 PE=1 SV=1)

HSP 1 Score: 422.2 bits (1084), Expect = 7.3e-117
Identity = 219/371 (59.03%), Postives = 264/371 (71.16%), Query Frame = 0

Query: 64  SRIADNYQSLDEVSAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQNPY 123
           S IAD++ SLD+V  +L +AGLESSNLI+GIDFTKSNEWTG  SFNR+SLH IG  QNPY
Sbjct: 12  SYIADHFSSLDQVITSLREAGLESSNLILGIDFTKSNEWTGRYSFNRKSLHAIGKRQNPY 71

Query: 124 EQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQDVFSFYDDDRICNGFEEVLHRYREIVP 183
           E+AISIIG+TLS FDED++IPC+GFGD +T DQ VFSFY +++ C+G E  + RYREIVP
Sbjct: 72  EKAISIIGRTLSPFDEDDLIPCFGFGDVTTRDQYVFSFYPENKSCDGLENAVKRYREIVP 131

Query: 184 NLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTHNGQLSSQERKTVDA 243
           +L+L+GPTSFAP+I+ A+ IVEQ   QYHVL+IIADGQVTR+ D   G+LS QE  T+++
Sbjct: 132 HLKLSGPTSFAPVIDAAINIVEQNNMQYHVLVIIADGQVTRNPDVPLGRLSPQEEATMNS 191

Query: 244 IVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDNFQFVNFTEIMSKSVNSARRQTE 303
           I+ AS YPLSI+LVGVGDGPWD MK FDDNIP R FDNFQFVNFT+IMS+  ++A+++  
Sbjct: 192 IMAASHYPLSIVLVGVGDGPWDTMKQFDDNIPHREFDNFQFVNFTKIMSEHKDAAKKEAA 251

Query: 304 FALSALMEIPSQYQATLELGLLGRQRGSSPERVPLPPPLYGATPSNGYLRTPRSNSFQTR 363
           FAL+ALMEIP QY+ATL L      R S     PLPPP       N     P        
Sbjct: 252 FALAALMEIPFQYKATLSLN-RKPVRSSHQHHKPLPPPPEVIERDNAVRSVP-------- 311

Query: 364 TPSHSRYNQGVGTTTSSSSHYDN--QVCPICLTNPKDMAFGCGHQTCCDCGETLELCPIC 423
                  NQ   T   S     +   VCPICLTNPKDMAF CGH TC +CG  +  CP+C
Sbjct: 312 -------NQMTETAEKSDRLAPSTVPVCPICLTNPKDMAFSCGHTTCKECGVVITTCPLC 366

Query: 424 RSFIQTRIRLY 433
           R  I TRIRLY
Sbjct: 372 RQPITTRIRLY 366

BLAST of Tan0008795 vs. NCBI nr
Match: XP_022966892.1 (E3 ubiquitin-protein ligase RGLG2-like [Cucurbita maxima])

HSP 1 Score: 803.5 bits (2074), Expect = 8.8e-229
Identity = 396/432 (91.67%), Postives = 414/432 (95.83%), Query Frame = 0

Query: 1   MGGKNSKDSGQREFDSYGSGNSSTWDHYNYPPPSPYVQQYSYPQPSDYGSQTPQPQRRLD 60
           MG KNSK++ QREFDS G GNSSTW + NYPPPSPYVQQYSYPQ SDYGSQTP PQRRLD
Sbjct: 1   MGSKNSKETDQREFDSDGFGNSSTWGYDNYPPPSPYVQQYSYPQRSDYGSQTPLPQRRLD 60

Query: 61  RRYSRIADNYQSLDEVSAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQ 120
           R+YSRIAD+YQSLDEV+AALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGN Q
Sbjct: 61  RKYSRIADHYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNTQ 120

Query: 121 NPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQDVFSFYDDDRICNGFEEVLHRYRE 180
           NPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQ+VFSFYD+DRICNGFEEVLHRY+E
Sbjct: 121 NPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQNVFSFYDNDRICNGFEEVLHRYKE 180

Query: 181 IVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTHNGQLSSQERKT 240
           IVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDT+NGQLSSQER T
Sbjct: 181 IVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTNNGQLSSQERNT 240

Query: 241 VDAIVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDNFQFVNFTEIMSKSVNSARR 300
           VDAIVRASEYPLSIILVGVGDGPWDMMK+FDDNIPARAFDNFQFVNFTEIMSK+VNS RR
Sbjct: 241 VDAIVRASEYPLSIILVGVGDGPWDMMKNFDDNIPARAFDNFQFVNFTEIMSKNVNSTRR 300

Query: 301 QTEFALSALMEIPSQYQATLELGLLGRQRGSSPERVPLPPPLYGATPSNGYLRTPRSNSF 360
           QTEFAL+ALMEIPSQYQATLELGLLGR+RGSSPERVPLPPPLY  TP NGY RTPR+NSF
Sbjct: 301 QTEFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYSMTPFNGYPRTPRANSF 360

Query: 361 QTRTPSHSRYNQGVGTTTSSSSHYDNQVCPICLTNPKDMAFGCGHQTCCDCGETLELCPI 420
           QTRTPSH RYNQGVGTT+S+SSHYDNQ+CPICLTNPKDMAFGCGHQTCCDCGETLE CPI
Sbjct: 361 QTRTPSHGRYNQGVGTTSSTSSHYDNQICPICLTNPKDMAFGCGHQTCCDCGETLESCPI 420

Query: 421 CRSFIQTRIRLY 433
           CRS IQTRIRLY
Sbjct: 421 CRSSIQTRIRLY 432

BLAST of Tan0008795 vs. NCBI nr
Match: XP_023542441.1 (E3 ubiquitin-protein ligase RGLG2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 802.4 bits (2071), Expect = 2.0e-228
Identity = 395/432 (91.44%), Postives = 414/432 (95.83%), Query Frame = 0

Query: 1   MGGKNSKDSGQREFDSYGSGNSSTWDHYNYPPPSPYVQQYSYPQPSDYGSQTPQPQRRLD 60
           MG KNSK++ QREFDS G GNSSTW + NYPPPSPYV+QYSYPQ SDYGSQTP PQRRLD
Sbjct: 1   MGSKNSKETDQREFDSDGFGNSSTWGYDNYPPPSPYVEQYSYPQRSDYGSQTPLPQRRLD 60

Query: 61  RRYSRIADNYQSLDEVSAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQ 120
           R+YSRIAD+YQSLDEV+AALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGN Q
Sbjct: 61  RKYSRIADHYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNVQ 120

Query: 121 NPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQDVFSFYDDDRICNGFEEVLHRYRE 180
           NPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQ+VFSFYD+DRICNGFEEVLHRY+E
Sbjct: 121 NPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQNVFSFYDNDRICNGFEEVLHRYKE 180

Query: 181 IVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTHNGQLSSQERKT 240
           IVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDT+NGQLSSQER T
Sbjct: 181 IVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTNNGQLSSQERNT 240

Query: 241 VDAIVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDNFQFVNFTEIMSKSVNSARR 300
           VDAIVRASEYPLSIILVGVGDGPWDMMK+FDDNIPARAFDNFQFVNFTEIMSK+VNS RR
Sbjct: 241 VDAIVRASEYPLSIILVGVGDGPWDMMKNFDDNIPARAFDNFQFVNFTEIMSKNVNSTRR 300

Query: 301 QTEFALSALMEIPSQYQATLELGLLGRQRGSSPERVPLPPPLYGATPSNGYLRTPRSNSF 360
           QTEFAL+ALMEIPSQYQATLELGLLGR+RGSSPERVPLPPPLY  TP NGY RTPR+NSF
Sbjct: 301 QTEFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYSTTPFNGYPRTPRANSF 360

Query: 361 QTRTPSHSRYNQGVGTTTSSSSHYDNQVCPICLTNPKDMAFGCGHQTCCDCGETLELCPI 420
           QTRTPSH RYNQGVGTT+S+SSHYDNQ+CPICLTNPKDMAFGCGHQTCCDCGETLE CPI
Sbjct: 361 QTRTPSHGRYNQGVGTTSSTSSHYDNQICPICLTNPKDMAFGCGHQTCCDCGETLESCPI 420

Query: 421 CRSFIQTRIRLY 433
           CRS IQTRIRLY
Sbjct: 421 CRSSIQTRIRLY 432

BLAST of Tan0008795 vs. NCBI nr
Match: KAG6573681.1 (E3 ubiquitin-protein ligase RGLG2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 801.6 bits (2069), Expect = 3.4e-228
Identity = 394/432 (91.20%), Postives = 414/432 (95.83%), Query Frame = 0

Query: 1   MGGKNSKDSGQREFDSYGSGNSSTWDHYNYPPPSPYVQQYSYPQPSDYGSQTPQPQRRLD 60
           MG +NSK++ QREFDS G GNSSTW + NYPPPSPYVQ+YSYPQ SDYGSQTP PQRRLD
Sbjct: 1   MGSRNSKETDQREFDSDGFGNSSTWGYDNYPPPSPYVQEYSYPQRSDYGSQTPLPQRRLD 60

Query: 61  RRYSRIADNYQSLDEVSAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQ 120
           R+YSRIAD+YQSLDEV+AALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGN Q
Sbjct: 61  RKYSRIADHYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNTQ 120

Query: 121 NPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQDVFSFYDDDRICNGFEEVLHRYRE 180
           NPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQ+VFSFYD+DRICNGFEEVLHRY+E
Sbjct: 121 NPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQNVFSFYDNDRICNGFEEVLHRYKE 180

Query: 181 IVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTHNGQLSSQERKT 240
           IVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDT+NGQLSSQER T
Sbjct: 181 IVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTNNGQLSSQERNT 240

Query: 241 VDAIVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDNFQFVNFTEIMSKSVNSARR 300
           VDAIVRASEYPLSIILVGVGDGPWDMMK+FDDNIPARAFDNFQFVNFTEIMSK+VNS RR
Sbjct: 241 VDAIVRASEYPLSIILVGVGDGPWDMMKNFDDNIPARAFDNFQFVNFTEIMSKNVNSTRR 300

Query: 301 QTEFALSALMEIPSQYQATLELGLLGRQRGSSPERVPLPPPLYGATPSNGYLRTPRSNSF 360
           QTEFAL+ALMEIPSQYQATLELGLLGR+RGSSPERVPLPPPLY  TP NGY RTPR+NSF
Sbjct: 301 QTEFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYSTTPFNGYPRTPRANSF 360

Query: 361 QTRTPSHSRYNQGVGTTTSSSSHYDNQVCPICLTNPKDMAFGCGHQTCCDCGETLELCPI 420
           QTRTPSH RYNQGVGTT+S+SSHYDNQ+CPICLTNPKDMAFGCGHQTCCDCGETLE CPI
Sbjct: 361 QTRTPSHGRYNQGVGTTSSTSSHYDNQICPICLTNPKDMAFGCGHQTCCDCGETLESCPI 420

Query: 421 CRSFIQTRIRLY 433
           CRS IQTRIRLY
Sbjct: 421 CRSSIQTRIRLY 432

BLAST of Tan0008795 vs. NCBI nr
Match: XP_022945486.1 (E3 ubiquitin-protein ligase RGLG2-like [Cucurbita moschata])

HSP 1 Score: 800.0 bits (2065), Expect = 9.8e-228
Identity = 394/432 (91.20%), Postives = 412/432 (95.37%), Query Frame = 0

Query: 1   MGGKNSKDSGQREFDSYGSGNSSTWDHYNYPPPSPYVQQYSYPQPSDYGSQTPQPQRRLD 60
           MG KNSK++ QREFDS G GNSSTW + NYPPPSPYVQ+YSYPQ  DYGSQTP PQRRLD
Sbjct: 1   MGSKNSKETDQREFDSDGFGNSSTWGYDNYPPPSPYVQEYSYPQRFDYGSQTPLPQRRLD 60

Query: 61  RRYSRIADNYQSLDEVSAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQ 120
           R+YSRIAD+YQSLDEV+AALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGN Q
Sbjct: 61  RKYSRIADHYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNTQ 120

Query: 121 NPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQDVFSFYDDDRICNGFEEVLHRYRE 180
           NPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQ+VFSFYDDDRICNGFEEVLHRY+E
Sbjct: 121 NPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQNVFSFYDDDRICNGFEEVLHRYKE 180

Query: 181 IVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTHNGQLSSQERKT 240
           IVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDT+NGQLSSQER T
Sbjct: 181 IVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTNNGQLSSQERNT 240

Query: 241 VDAIVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDNFQFVNFTEIMSKSVNSARR 300
           VDAIVRASEYPLSIILVGVGDGPWDMMK+FDDNIPARAFDNFQFVNFTEIMSK+VNS RR
Sbjct: 241 VDAIVRASEYPLSIILVGVGDGPWDMMKNFDDNIPARAFDNFQFVNFTEIMSKNVNSTRR 300

Query: 301 QTEFALSALMEIPSQYQATLELGLLGRQRGSSPERVPLPPPLYGATPSNGYLRTPRSNSF 360
           QTEFAL+ALMEIPSQYQATLELGLLGR+RGSSPERVPLPPPLY  TP N Y RTPR+NSF
Sbjct: 301 QTEFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYSTTPFNSYPRTPRANSF 360

Query: 361 QTRTPSHSRYNQGVGTTTSSSSHYDNQVCPICLTNPKDMAFGCGHQTCCDCGETLELCPI 420
           QTRTPSH RYNQGVGTT+S+SSHYDNQ+CPICLTNPKDMAFGCGHQTCCDCGETLE CPI
Sbjct: 361 QTRTPSHGRYNQGVGTTSSTSSHYDNQICPICLTNPKDMAFGCGHQTCCDCGETLESCPI 420

Query: 421 CRSFIQTRIRLY 433
           CRS IQTRIRLY
Sbjct: 421 CRSSIQTRIRLY 432

BLAST of Tan0008795 vs. NCBI nr
Match: XP_038892155.1 (E3 ubiquitin-protein ligase RGLG2-like [Benincasa hispida] >XP_038892156.1 E3 ubiquitin-protein ligase RGLG2-like [Benincasa hispida])

HSP 1 Score: 785.4 bits (2027), Expect = 2.5e-223
Identity = 383/432 (88.66%), Postives = 410/432 (94.91%), Query Frame = 0

Query: 1   MGGKNSKDSGQREFDSYGSGNSSTWDHYNYPPPSPYVQQYSYPQPSDYGSQTPQPQRRLD 60
           MGGKNS++ GQRE  SYGSGNSSTWD YN+PPPSP+V QY Y +PSDYGSQTPQPQRRLD
Sbjct: 1   MGGKNSREGGQRELYSYGSGNSSTWDQYNFPPPSPHVHQYPYAEPSDYGSQTPQPQRRLD 60

Query: 61  RRYSRIADNYQSLDEVSAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQ 120
           R+YSRIADNYQSLDEV+AALA+AGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQ
Sbjct: 61  RKYSRIADNYQSLDEVTAALAKAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQ 120

Query: 121 NPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQDVFSFYDDDRICNGFEEVLHRYRE 180
           NPYEQAISIIGQTLSAFDEDN+IPC+GFGDASTHDQDVFSFY+DDRICNGFEEVLHRYRE
Sbjct: 121 NPYEQAISIIGQTLSAFDEDNLIPCFGFGDASTHDQDVFSFYEDDRICNGFEEVLHRYRE 180

Query: 181 IVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTHNGQLSSQERKT 240
           IVPNL+LAGPTSFAPIIEMAMTIVEQ+GGQYHVLLIIADGQVTRSVDTHNGQLS QE+K+
Sbjct: 181 IVPNLKLAGPTSFAPIIEMAMTIVEQSGGQYHVLLIIADGQVTRSVDTHNGQLSLQEQKS 240

Query: 241 VDAIVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDNFQFVNFTEIMSKSVNSARR 300
           +DAIVRASEYPLSIILVGVGDGPWDMMK+FDDNIPARAFDNFQFVNFTE+MSK+VN+ RR
Sbjct: 241 IDAIVRASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEMMSKNVNNVRR 300

Query: 301 QTEFALSALMEIPSQYQATLELGLLGRQRGSSPERVPLPPPLYGATPSNGYLRTPRSNSF 360
           QTEF+L+ALMEIPSQY ATLELGLLGRQRG SPERVPLPPP+Y     NGY RTPRSN+F
Sbjct: 301 QTEFSLAALMEIPSQYLATLELGLLGRQRGISPERVPLPPPVY-----NGYSRTPRSNNF 360

Query: 361 QTRTPSHSRYNQGVGTTTSSSSHYDNQVCPICLTNPKDMAFGCGHQTCCDCGETLELCPI 420
            TRTPS  RYNQGVGTT+SSSSHYDNQ+CPICLTNPKD+AFGCGH TCCDCGE LELCPI
Sbjct: 361 PTRTPSLGRYNQGVGTTSSSSSHYDNQLCPICLTNPKDLAFGCGHLTCCDCGENLELCPI 420

Query: 421 CRSFIQTRIRLY 433
           CRSFIQTRIRLY
Sbjct: 421 CRSFIQTRIRLY 427

BLAST of Tan0008795 vs. ExPASy TrEMBL
Match: A0A6J1HP79 (E3 ubiquitin-protein ligase RGLG2-like OS=Cucurbita maxima OX=3661 GN=LOC111466460 PE=4 SV=1)

HSP 1 Score: 803.5 bits (2074), Expect = 4.3e-229
Identity = 396/432 (91.67%), Postives = 414/432 (95.83%), Query Frame = 0

Query: 1   MGGKNSKDSGQREFDSYGSGNSSTWDHYNYPPPSPYVQQYSYPQPSDYGSQTPQPQRRLD 60
           MG KNSK++ QREFDS G GNSSTW + NYPPPSPYVQQYSYPQ SDYGSQTP PQRRLD
Sbjct: 1   MGSKNSKETDQREFDSDGFGNSSTWGYDNYPPPSPYVQQYSYPQRSDYGSQTPLPQRRLD 60

Query: 61  RRYSRIADNYQSLDEVSAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQ 120
           R+YSRIAD+YQSLDEV+AALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGN Q
Sbjct: 61  RKYSRIADHYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNTQ 120

Query: 121 NPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQDVFSFYDDDRICNGFEEVLHRYRE 180
           NPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQ+VFSFYD+DRICNGFEEVLHRY+E
Sbjct: 121 NPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQNVFSFYDNDRICNGFEEVLHRYKE 180

Query: 181 IVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTHNGQLSSQERKT 240
           IVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDT+NGQLSSQER T
Sbjct: 181 IVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTNNGQLSSQERNT 240

Query: 241 VDAIVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDNFQFVNFTEIMSKSVNSARR 300
           VDAIVRASEYPLSIILVGVGDGPWDMMK+FDDNIPARAFDNFQFVNFTEIMSK+VNS RR
Sbjct: 241 VDAIVRASEYPLSIILVGVGDGPWDMMKNFDDNIPARAFDNFQFVNFTEIMSKNVNSTRR 300

Query: 301 QTEFALSALMEIPSQYQATLELGLLGRQRGSSPERVPLPPPLYGATPSNGYLRTPRSNSF 360
           QTEFAL+ALMEIPSQYQATLELGLLGR+RGSSPERVPLPPPLY  TP NGY RTPR+NSF
Sbjct: 301 QTEFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYSMTPFNGYPRTPRANSF 360

Query: 361 QTRTPSHSRYNQGVGTTTSSSSHYDNQVCPICLTNPKDMAFGCGHQTCCDCGETLELCPI 420
           QTRTPSH RYNQGVGTT+S+SSHYDNQ+CPICLTNPKDMAFGCGHQTCCDCGETLE CPI
Sbjct: 361 QTRTPSHGRYNQGVGTTSSTSSHYDNQICPICLTNPKDMAFGCGHQTCCDCGETLESCPI 420

Query: 421 CRSFIQTRIRLY 433
           CRS IQTRIRLY
Sbjct: 421 CRSSIQTRIRLY 432

BLAST of Tan0008795 vs. ExPASy TrEMBL
Match: A0A6J1G0Z9 (E3 ubiquitin-protein ligase RGLG2-like OS=Cucurbita moschata OX=3662 GN=LOC111449704 PE=4 SV=1)

HSP 1 Score: 800.0 bits (2065), Expect = 4.7e-228
Identity = 394/432 (91.20%), Postives = 412/432 (95.37%), Query Frame = 0

Query: 1   MGGKNSKDSGQREFDSYGSGNSSTWDHYNYPPPSPYVQQYSYPQPSDYGSQTPQPQRRLD 60
           MG KNSK++ QREFDS G GNSSTW + NYPPPSPYVQ+YSYPQ  DYGSQTP PQRRLD
Sbjct: 1   MGSKNSKETDQREFDSDGFGNSSTWGYDNYPPPSPYVQEYSYPQRFDYGSQTPLPQRRLD 60

Query: 61  RRYSRIADNYQSLDEVSAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQ 120
           R+YSRIAD+YQSLDEV+AALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGN Q
Sbjct: 61  RKYSRIADHYQSLDEVTAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNTQ 120

Query: 121 NPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQDVFSFYDDDRICNGFEEVLHRYRE 180
           NPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQ+VFSFYDDDRICNGFEEVLHRY+E
Sbjct: 121 NPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQNVFSFYDDDRICNGFEEVLHRYKE 180

Query: 181 IVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTHNGQLSSQERKT 240
           IVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDT+NGQLSSQER T
Sbjct: 181 IVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTNNGQLSSQERNT 240

Query: 241 VDAIVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDNFQFVNFTEIMSKSVNSARR 300
           VDAIVRASEYPLSIILVGVGDGPWDMMK+FDDNIPARAFDNFQFVNFTEIMSK+VNS RR
Sbjct: 241 VDAIVRASEYPLSIILVGVGDGPWDMMKNFDDNIPARAFDNFQFVNFTEIMSKNVNSTRR 300

Query: 301 QTEFALSALMEIPSQYQATLELGLLGRQRGSSPERVPLPPPLYGATPSNGYLRTPRSNSF 360
           QTEFAL+ALMEIPSQYQATLELGLLGR+RGSSPERVPLPPPLY  TP N Y RTPR+NSF
Sbjct: 301 QTEFALAALMEIPSQYQATLELGLLGRRRGSSPERVPLPPPLYSTTPFNSYPRTPRANSF 360

Query: 361 QTRTPSHSRYNQGVGTTTSSSSHYDNQVCPICLTNPKDMAFGCGHQTCCDCGETLELCPI 420
           QTRTPSH RYNQGVGTT+S+SSHYDNQ+CPICLTNPKDMAFGCGHQTCCDCGETLE CPI
Sbjct: 361 QTRTPSHGRYNQGVGTTSSTSSHYDNQICPICLTNPKDMAFGCGHQTCCDCGETLESCPI 420

Query: 421 CRSFIQTRIRLY 433
           CRS IQTRIRLY
Sbjct: 421 CRSSIQTRIRLY 432

BLAST of Tan0008795 vs. ExPASy TrEMBL
Match: A0A1S3BDV1 (E3 ubiquitin-protein ligase RGLG2-like OS=Cucumis melo OX=3656 GN=LOC103488946 PE=4 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 2.1e-223
Identity = 390/435 (89.66%), Postives = 407/435 (93.56%), Query Frame = 0

Query: 1   MGGKNSKDSGQREFDSYGSGNSSTWDHYNYPP--PSPYVQQYSYPQPSDYGSQTP-QPQR 60
           MGGKNS++ GQREF SYGSGNSSTWD YNYPP  PSPYVQQY Y +PS YGSQTP QP+R
Sbjct: 1   MGGKNSREVGQREFGSYGSGNSSTWDQYNYPPPSPSPYVQQYPYTEPSGYGSQTPQQPRR 60

Query: 61  RLDRRYSRIADNYQSLDEVSAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG 120
           RLDR+YSRIADNYQSLDEV+AALA AGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG
Sbjct: 61  RLDRKYSRIADNYQSLDEVTAALANAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG 120

Query: 121 NGQNPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQDVFSFYDDDRICNGFEEVLHR 180
           N  NPYEQAISIIGQTLSAFDEDN+IPC+GFGDASTHDQDVFSFY DDRICNGFEEVLHR
Sbjct: 121 NEPNPYEQAISIIGQTLSAFDEDNLIPCFGFGDASTHDQDVFSFYHDDRICNGFEEVLHR 180

Query: 181 YREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTHNGQLSSQE 240
           YREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTHNGQLS QE
Sbjct: 181 YREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTHNGQLSLQE 240

Query: 241 RKTVDAIVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDNFQFVNFTEIMSKSVNS 300
           RKT+DAIVRASEYPLSII VGVGDGPWDMMK+FDDNIPARAFDNFQFVNFTEIM K+VN+
Sbjct: 241 RKTIDAIVRASEYPLSIIFVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMLKNVNN 300

Query: 301 ARRQTEFALSALMEIPSQYQATLELGLLGRQRGSSPERVPLPPPLYGATPSNGYLRTPRS 360
            RRQTEFAL+ALMEIPSQYQATLELG+LGRQRGSSPERVPLPPPLY AT S+GY RTP S
Sbjct: 301 VRRQTEFALAALMEIPSQYQATLELGILGRQRGSSPERVPLPPPLYSATSSSGYPRTPCS 360

Query: 361 NSFQTRTPSHSRYNQGVGTTTSSSSHYDNQVCPICLTNPKDMAFGCGHQTCCDCGETLEL 420
           NSFQTRTPS  RYNQGVGT  SSSS YDNQ+CPIC+TNPKDMAFGCGH TCCDCGE LEL
Sbjct: 361 NSFQTRTPSLGRYNQGVGTNPSSSSPYDNQLCPICITNPKDMAFGCGHMTCCDCGENLEL 420

Query: 421 CPICRSFIQTRIRLY 433
           CPICRSFIQTRIRLY
Sbjct: 421 CPICRSFIQTRIRLY 435

BLAST of Tan0008795 vs. ExPASy TrEMBL
Match: A0A5D3CUT6 (E3 ubiquitin-protein ligase RGLG2-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G001680 PE=4 SV=1)

HSP 1 Score: 784.6 bits (2025), Expect = 2.1e-223
Identity = 390/435 (89.66%), Postives = 407/435 (93.56%), Query Frame = 0

Query: 1   MGGKNSKDSGQREFDSYGSGNSSTWDHYNYPP--PSPYVQQYSYPQPSDYGSQTP-QPQR 60
           MGGKNS++ GQREF SYGSGNSSTWD YNYPP  PSPYVQQY Y +PS YGSQTP QP+R
Sbjct: 1   MGGKNSREVGQREFGSYGSGNSSTWDQYNYPPPSPSPYVQQYPYTEPSGYGSQTPQQPRR 60

Query: 61  RLDRRYSRIADNYQSLDEVSAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG 120
           RLDR+YSRIADNYQSLDEV+AALA AGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG
Sbjct: 61  RLDRKYSRIADNYQSLDEVTAALANAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIG 120

Query: 121 NGQNPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQDVFSFYDDDRICNGFEEVLHR 180
           N  NPYEQAISIIGQTLSAFDEDN+IPC+GFGDASTHDQDVFSFY DDRICNGFEEVLHR
Sbjct: 121 NEPNPYEQAISIIGQTLSAFDEDNLIPCFGFGDASTHDQDVFSFYHDDRICNGFEEVLHR 180

Query: 181 YREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTHNGQLSSQE 240
           YREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTHNGQLS QE
Sbjct: 181 YREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTHNGQLSLQE 240

Query: 241 RKTVDAIVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDNFQFVNFTEIMSKSVNS 300
           RKT+DAIVRASEYPLSII VGVGDGPWDMMK+FDDNIPARAFDNFQFVNFTEIM K+VN+
Sbjct: 241 RKTIDAIVRASEYPLSIIFVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMLKNVNN 300

Query: 301 ARRQTEFALSALMEIPSQYQATLELGLLGRQRGSSPERVPLPPPLYGATPSNGYLRTPRS 360
            RRQTEFAL+ALMEIPSQYQATLELG+LGRQRGSSPERVPLPPPLY AT S+GY RTP S
Sbjct: 301 VRRQTEFALAALMEIPSQYQATLELGILGRQRGSSPERVPLPPPLYSATSSSGYPRTPCS 360

Query: 361 NSFQTRTPSHSRYNQGVGTTTSSSSHYDNQVCPICLTNPKDMAFGCGHQTCCDCGETLEL 420
           NSFQTRTPS  RYNQGVGT  SSSS YDNQ+CPIC+TNPKDMAFGCGH TCCDCGE LEL
Sbjct: 361 NSFQTRTPSLGRYNQGVGTNPSSSSPYDNQLCPICITNPKDMAFGCGHMTCCDCGENLEL 420

Query: 421 CPICRSFIQTRIRLY 433
           CPICRSFIQTRIRLY
Sbjct: 421 CPICRSFIQTRIRLY 435

BLAST of Tan0008795 vs. ExPASy TrEMBL
Match: A0A6J1DFL9 (E3 ubiquitin-protein ligase RGLG2-like OS=Momordica charantia OX=3673 GN=LOC111019656 PE=4 SV=1)

HSP 1 Score: 781.6 bits (2017), Expect = 1.7e-222
Identity = 378/432 (87.50%), Postives = 406/432 (93.98%), Query Frame = 0

Query: 1   MGGKNSKDSGQREFDSYGSGNSSTWDHYNYPPPSPYVQQYSYPQPSDYGSQTPQPQRRLD 60
           MGG+NSK+  +REFDSYG  NS+ WD ++YPPPSPY QQY Y   SDYGSQTPQPQRRLD
Sbjct: 1   MGGRNSKEPSRREFDSYGYDNSAAWDQFSYPPPSPYPQQYPYTPSSDYGSQTPQPQRRLD 60

Query: 61  RRYSRIADNYQSLDEVSAALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQ 120
           R+YSRIADNY SLDEV++ALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQ
Sbjct: 61  RKYSRIADNYSSLDEVTSALAQAGLESSNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQ 120

Query: 121 NPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQDVFSFYDDDRICNGFEEVLHRYRE 180
           NPYEQAISIIGQTLS FDEDN+IPC+GFGDASTHDQDVFSFY+ DR CNGFEEVLHRYRE
Sbjct: 121 NPYEQAISIIGQTLSVFDEDNLIPCFGFGDASTHDQDVFSFYEHDRTCNGFEEVLHRYRE 180

Query: 181 IVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVDTHNGQLSSQERKT 240
           +VPNLRLAGPTSFAPI+EMA TIVE+TGGQYHVLLIIADGQVTRSVDTH+GQLS QERKT
Sbjct: 181 VVPNLRLAGPTSFAPIVEMATTIVEKTGGQYHVLLIIADGQVTRSVDTHHGQLSPQERKT 240

Query: 241 VDAIVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDNFQFVNFTEIMSKSVNSARR 300
           +DAIV+ASEYPLSIILVGVGDGPWDMMK+FDDNIPARAFDNFQFVNFTEIMSK+VN +RR
Sbjct: 241 IDAIVKASEYPLSIILVGVGDGPWDMMKEFDDNIPARAFDNFQFVNFTEIMSKNVNPSRR 300

Query: 301 QTEFALSALMEIPSQYQATLELGLLGRQRGSSPERVPLPPPLYGATPSNGYLRTPRSNSF 360
           QTEFAL+ALMEIPSQY+ATLELGLLGRQRGSSPERVPLPPPLYGA PSNGY RT  SNSF
Sbjct: 301 QTEFALAALMEIPSQYKATLELGLLGRQRGSSPERVPLPPPLYGAAPSNGYSRTSHSNSF 360

Query: 361 QTRTPSHSRYNQGVGTTTSSSSHYDNQVCPICLTNPKDMAFGCGHQTCCDCGETLELCPI 420
           Q+RTP+HSR N+GVGT  SSSSHYDNQ+CPICLTNPKDMAFGCGHQTCCDCGE LELCPI
Sbjct: 361 QSRTPAHSRNNKGVGTAPSSSSHYDNQLCPICLTNPKDMAFGCGHQTCCDCGENLELCPI 420

Query: 421 CRSFIQTRIRLY 433
           CRSFIQTRI+LY
Sbjct: 421 CRSFIQTRIKLY 432

BLAST of Tan0008795 vs. TAIR 10
Match: AT5G14420.1 (RING domain ligase2 )

HSP 1 Score: 568.9 bits (1465), Expect = 3.4e-162
Identity = 298/468 (63.68%), Postives = 359/468 (76.71%), Query Frame = 0

Query: 1   MGGKNSKDSGQRE-----FDSYGSGNSSTW-----------DHYNYPPPSPYVQ--QYSY 60
           MG  NSK++ ++        S  S +SS+W           + YNYPPP  Y Q  +Y+ 
Sbjct: 1   MGTGNSKENWRQSSFRSTSASSASPSSSSWASQQSYPQYGAESYNYPPPPSYAQPPEYTQ 60

Query: 61  PQPSDYGSQ--------TPQPQ-------RRLDRRYSRIADNYQSLDEVSAALAQAGLES 120
           P P  Y +Q         P  Q       +RL+R+YS+I+D+Y SL++V+ ALA+AGLES
Sbjct: 61  PPPPLYSTQPYSAPSYSAPPSQSYGSDNKKRLERKYSKISDDYSSLEQVTEALARAGLES 120

Query: 121 SNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQNPYEQAISIIGQTLSAFDEDNMIPCYG 180
           SNLIVGIDFTKSNEWTG+RSFNR+SLH IG+  NPYEQAI+IIG+TL+AFDEDN+IPCYG
Sbjct: 121 SNLIVGIDFTKSNEWTGARSFNRKSLHFIGSSPNPYEQAITIIGRTLAAFDEDNLIPCYG 180

Query: 181 FGDASTHDQDVFSFYDDDRICNGFEEVLHRYREIVPNLRLAGPTSFAPIIEMAMTIVEQT 240
           FGDASTHDQDVFSF  +DR CNGFEEVL RY+EIVP L+LAGPTSFAPII+MAMTIVEQ+
Sbjct: 181 FGDASTHDQDVFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQS 240

Query: 241 GGQYHVLLIIADGQVTRSVDTHNGQLSSQERKTVDAIVRASEYPLSIILVGVGDGPWDMM 300
           GGQYHVL+IIADGQVTRSVDT NGQLS QE+KTVDAIV+AS+ PLSI+LVGVGDGPWDMM
Sbjct: 241 GGQYHVLVIIADGQVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWDMM 300

Query: 301 KDFDDNIPARAFDNFQFVNFTEIMSKSVNSARRQTEFALSALMEIPSQYQATLELGLLGR 360
           ++FDDNIPARAFDNFQFVNFTEIM+K+   + ++TEFALSALMEIP QY+AT+EL LLGR
Sbjct: 301 REFDDNIPARAFDNFQFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIELNLLGR 360

Query: 361 QRGSSPERVPLPPPLYGATPSNGYLRTPRSNSFQTRTPSHSRYNQGVGTT---TSSSSHY 420
           + G  PER PLPPP+ G + S    +  R  SF+   P H      V ++     +SS  
Sbjct: 361 RNGYIPERFPLPPPMRGGSSSYNSPKPSRLPSFKPSVPPHPTEGYHVRSSPVPPPTSSAS 420

Query: 421 DNQVCPICLTNPKDMAFGCGHQTCCDCGETLELCPICRSFIQTRIRLY 433
           DNQ+CPICL+NPKDMAFGCGHQTCC+CG  L++CPICR+ IQTRI+LY
Sbjct: 421 DNQLCPICLSNPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKLY 468

BLAST of Tan0008795 vs. TAIR 10
Match: AT5G14420.3 (RING domain ligase2 )

HSP 1 Score: 568.9 bits (1465), Expect = 3.4e-162
Identity = 298/468 (63.68%), Postives = 359/468 (76.71%), Query Frame = 0

Query: 1   MGGKNSKDSGQRE-----FDSYGSGNSSTW-----------DHYNYPPPSPYVQ--QYSY 60
           MG  NSK++ ++        S  S +SS+W           + YNYPPP  Y Q  +Y+ 
Sbjct: 1   MGTGNSKENWRQSSFRSTSASSASPSSSSWASQQSYPQYGAESYNYPPPPSYAQPPEYTQ 60

Query: 61  PQPSDYGSQ--------TPQPQ-------RRLDRRYSRIADNYQSLDEVSAALAQAGLES 120
           P P  Y +Q         P  Q       +RL+R+YS+I+D+Y SL++V+ ALA+AGLES
Sbjct: 61  PPPPLYSTQPYSAPSYSAPPSQSYGSDNKKRLERKYSKISDDYSSLEQVTEALARAGLES 120

Query: 121 SNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQNPYEQAISIIGQTLSAFDEDNMIPCYG 180
           SNLIVGIDFTKSNEWTG+RSFNR+SLH IG+  NPYEQAI+IIG+TL+AFDEDN+IPCYG
Sbjct: 121 SNLIVGIDFTKSNEWTGARSFNRKSLHFIGSSPNPYEQAITIIGRTLAAFDEDNLIPCYG 180

Query: 181 FGDASTHDQDVFSFYDDDRICNGFEEVLHRYREIVPNLRLAGPTSFAPIIEMAMTIVEQT 240
           FGDASTHDQDVFSF  +DR CNGFEEVL RY+EIVP L+LAGPTSFAPII+MAMTIVEQ+
Sbjct: 181 FGDASTHDQDVFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQS 240

Query: 241 GGQYHVLLIIADGQVTRSVDTHNGQLSSQERKTVDAIVRASEYPLSIILVGVGDGPWDMM 300
           GGQYHVL+IIADGQVTRSVDT NGQLS QE+KTVDAIV+AS+ PLSI+LVGVGDGPWDMM
Sbjct: 241 GGQYHVLVIIADGQVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWDMM 300

Query: 301 KDFDDNIPARAFDNFQFVNFTEIMSKSVNSARRQTEFALSALMEIPSQYQATLELGLLGR 360
           ++FDDNIPARAFDNFQFVNFTEIM+K+   + ++TEFALSALMEIP QY+AT+EL LLGR
Sbjct: 301 REFDDNIPARAFDNFQFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIELNLLGR 360

Query: 361 QRGSSPERVPLPPPLYGATPSNGYLRTPRSNSFQTRTPSHSRYNQGVGTT---TSSSSHY 420
           + G  PER PLPPP+ G + S    +  R  SF+   P H      V ++     +SS  
Sbjct: 361 RNGYIPERFPLPPPMRGGSSSYNSPKPSRLPSFKPSVPPHPTEGYHVRSSPVPPPTSSAS 420

Query: 421 DNQVCPICLTNPKDMAFGCGHQTCCDCGETLELCPICRSFIQTRIRLY 433
           DNQ+CPICL+NPKDMAFGCGHQTCC+CG  L++CPICR+ IQTRI+LY
Sbjct: 421 DNQLCPICLSNPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKLY 468

BLAST of Tan0008795 vs. TAIR 10
Match: AT5G14420.4 (RING domain ligase2 )

HSP 1 Score: 568.9 bits (1465), Expect = 3.4e-162
Identity = 298/468 (63.68%), Postives = 359/468 (76.71%), Query Frame = 0

Query: 1   MGGKNSKDSGQRE-----FDSYGSGNSSTW-----------DHYNYPPPSPYVQ--QYSY 60
           MG  NSK++ ++        S  S +SS+W           + YNYPPP  Y Q  +Y+ 
Sbjct: 1   MGTGNSKENWRQSSFRSTSASSASPSSSSWASQQSYPQYGAESYNYPPPPSYAQPPEYTQ 60

Query: 61  PQPSDYGSQ--------TPQPQ-------RRLDRRYSRIADNYQSLDEVSAALAQAGLES 120
           P P  Y +Q         P  Q       +RL+R+YS+I+D+Y SL++V+ ALA+AGLES
Sbjct: 61  PPPPLYSTQPYSAPSYSAPPSQSYGSDNKKRLERKYSKISDDYSSLEQVTEALARAGLES 120

Query: 121 SNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQNPYEQAISIIGQTLSAFDEDNMIPCYG 180
           SNLIVGIDFTKSNEWTG+RSFNR+SLH IG+  NPYEQAI+IIG+TL+AFDEDN+IPCYG
Sbjct: 121 SNLIVGIDFTKSNEWTGARSFNRKSLHFIGSSPNPYEQAITIIGRTLAAFDEDNLIPCYG 180

Query: 181 FGDASTHDQDVFSFYDDDRICNGFEEVLHRYREIVPNLRLAGPTSFAPIIEMAMTIVEQT 240
           FGDASTHDQDVFSF  +DR CNGFEEVL RY+EIVP L+LAGPTSFAPII+MAMTIVEQ+
Sbjct: 181 FGDASTHDQDVFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQS 240

Query: 241 GGQYHVLLIIADGQVTRSVDTHNGQLSSQERKTVDAIVRASEYPLSIILVGVGDGPWDMM 300
           GGQYHVL+IIADGQVTRSVDT NGQLS QE+KTVDAIV+AS+ PLSI+LVGVGDGPWDMM
Sbjct: 241 GGQYHVLVIIADGQVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWDMM 300

Query: 301 KDFDDNIPARAFDNFQFVNFTEIMSKSVNSARRQTEFALSALMEIPSQYQATLELGLLGR 360
           ++FDDNIPARAFDNFQFVNFTEIM+K+   + ++TEFALSALMEIP QY+AT+EL LLGR
Sbjct: 301 REFDDNIPARAFDNFQFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIELNLLGR 360

Query: 361 QRGSSPERVPLPPPLYGATPSNGYLRTPRSNSFQTRTPSHSRYNQGVGTT---TSSSSHY 420
           + G  PER PLPPP+ G + S    +  R  SF+   P H      V ++     +SS  
Sbjct: 361 RNGYIPERFPLPPPMRGGSSSYNSPKPSRLPSFKPSVPPHPTEGYHVRSSPVPPPTSSAS 420

Query: 421 DNQVCPICLTNPKDMAFGCGHQTCCDCGETLELCPICRSFIQTRIRLY 433
           DNQ+CPICL+NPKDMAFGCGHQTCC+CG  L++CPICR+ IQTRI+LY
Sbjct: 421 DNQLCPICLSNPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKLY 468

BLAST of Tan0008795 vs. TAIR 10
Match: AT5G14420.2 (RING domain ligase2 )

HSP 1 Score: 568.9 bits (1465), Expect = 3.4e-162
Identity = 298/468 (63.68%), Postives = 359/468 (76.71%), Query Frame = 0

Query: 1   MGGKNSKDSGQRE-----FDSYGSGNSSTW-----------DHYNYPPPSPYVQ--QYSY 60
           MG  NSK++ ++        S  S +SS+W           + YNYPPP  Y Q  +Y+ 
Sbjct: 1   MGTGNSKENWRQSSFRSTSASSASPSSSSWASQQSYPQYGAESYNYPPPPSYAQPPEYTQ 60

Query: 61  PQPSDYGSQ--------TPQPQ-------RRLDRRYSRIADNYQSLDEVSAALAQAGLES 120
           P P  Y +Q         P  Q       +RL+R+YS+I+D+Y SL++V+ ALA+AGLES
Sbjct: 61  PPPPLYSTQPYSAPSYSAPPSQSYGSDNKKRLERKYSKISDDYSSLEQVTEALARAGLES 120

Query: 121 SNLIVGIDFTKSNEWTGSRSFNRRSLHHIGNGQNPYEQAISIIGQTLSAFDEDNMIPCYG 180
           SNLIVGIDFTKSNEWTG+RSFNR+SLH IG+  NPYEQAI+IIG+TL+AFDEDN+IPCYG
Sbjct: 121 SNLIVGIDFTKSNEWTGARSFNRKSLHFIGSSPNPYEQAITIIGRTLAAFDEDNLIPCYG 180

Query: 181 FGDASTHDQDVFSFYDDDRICNGFEEVLHRYREIVPNLRLAGPTSFAPIIEMAMTIVEQT 240
           FGDASTHDQDVFSF  +DR CNGFEEVL RY+EIVP L+LAGPTSFAPII+MAMTIVEQ+
Sbjct: 181 FGDASTHDQDVFSFNSEDRFCNGFEEVLSRYKEIVPQLKLAGPTSFAPIIDMAMTIVEQS 240

Query: 241 GGQYHVLLIIADGQVTRSVDTHNGQLSSQERKTVDAIVRASEYPLSIILVGVGDGPWDMM 300
           GGQYHVL+IIADGQVTRSVDT NGQLS QE+KTVDAIV+AS+ PLSI+LVGVGDGPWDMM
Sbjct: 241 GGQYHVLVIIADGQVTRSVDTENGQLSPQEQKTVDAIVQASKLPLSIVLVGVGDGPWDMM 300

Query: 301 KDFDDNIPARAFDNFQFVNFTEIMSKSVNSARRQTEFALSALMEIPSQYQATLELGLLGR 360
           ++FDDNIPARAFDNFQFVNFTEIM+K+   + ++TEFALSALMEIP QY+AT+EL LLGR
Sbjct: 301 REFDDNIPARAFDNFQFVNFTEIMAKNKAQSLKETEFALSALMEIPQQYKATIELNLLGR 360

Query: 361 QRGSSPERVPLPPPLYGATPSNGYLRTPRSNSFQTRTPSHSRYNQGVGTT---TSSSSHY 420
           + G  PER PLPPP+ G + S    +  R  SF+   P H      V ++     +SS  
Sbjct: 361 RNGYIPERFPLPPPMRGGSSSYNSPKPSRLPSFKPSVPPHPTEGYHVRSSPVPPPTSSAS 420

Query: 421 DNQVCPICLTNPKDMAFGCGHQTCCDCGETLELCPICRSFIQTRIRLY 433
           DNQ+CPICL+NPKDMAFGCGHQTCC+CG  L++CPICR+ IQTRI+LY
Sbjct: 421 DNQLCPICLSNPKDMAFGCGHQTCCECGPDLQMCPICRAPIQTRIKLY 468

BLAST of Tan0008795 vs. TAIR 10
Match: AT1G67800.1 (Copine (Calcium-dependent phospholipid-binding protein) family )

HSP 1 Score: 550.4 bits (1417), Expect = 1.3e-156
Identity = 289/447 (64.65%), Postives = 343/447 (76.73%), Query Frame = 0

Query: 1   MGGKNSKDSGQ-----REFDSYGSGN---SSTWDHYNYPPPSPYVQQYSYPQPS-----D 60
           MGG +SK+S +     R ++   SG+   SS WD       S Y Q  ++P  S     +
Sbjct: 1   MGGSSSKESPRGGGSGRRYERSVSGSSSYSSAWDQ------SSYYQTPNHPSASPVSSYN 60

Query: 61  YGSQTPQPQRRLDRRYSRIADNYQSLDEVSAALAQAGLESSNLIVGIDFTKSNEWTGSRS 120
            G QTP   + L+R+YSRIADNY+S+DEV+AAL+ AGLESSNLIVGID TKSNEWTG+RS
Sbjct: 61  SGRQTP---KNLERKYSRIADNYRSIDEVTAALSHAGLESSNLIVGIDVTKSNEWTGARS 120

Query: 121 FNRRSLHHIGNGQNPYEQAISIIGQTLSAFDEDNMIPCYGFGDASTHDQDVFSFYDDDRI 180
           F R+SLH IG   NPY+QAISIIG+TLS FDEDN+IPCYGFGDA+THDQDVFSF  +D  
Sbjct: 121 FGRKSLHFIGTTPNPYQQAISIIGKTLSVFDEDNLIPCYGFGDATTHDQDVFSFNPNDTY 180

Query: 181 CNGFEEVLHRYREIVPNLRLAGPTSFAPIIEMAMTIVEQTGGQYHVLLIIADGQVTRSVD 240
           CNGFEEVL  YREIVP LRL+GPTSFAPIIE AMTIVE++GGQYHVLLIIADGQVTRSVD
Sbjct: 181 CNGFEEVLMCYREIVPQLRLSGPTSFAPIIERAMTIVEESGGQYHVLLIIADGQVTRSVD 240

Query: 241 THNGQLSSQERKTVDAIVRASEYPLSIILVGVGDGPWDMMKDFDDNIPARAFDNFQFVNF 300
           T NG  S QE++T+DAIVRASEYPLSI+LVGVGDGPWD M+ FDDNIPARAFDNFQFVNF
Sbjct: 241 TDNGGFSPQEQQTIDAIVRASEYPLSIVLVGVGDGPWDTMRQFDDNIPARAFDNFQFVNF 300

Query: 301 TEIMSKSVNSARRQTEFALSALMEIPSQYQATLELGLLGRQRGSSPERVPLPPPLYGATP 360
           T+IMSK+++ AR++ EFALSALMEIPSQY+ATLELGLLG++ G  P+R+ LPPP Y    
Sbjct: 301 TDIMSKNIDPARKEAEFALSALMEIPSQYKATLELGLLGQRTGHCPDRIALPPPTYATQS 360

Query: 361 SNGYLRTPRSNSFQTRTPSHSRYNQGVGTTTSSSSHYDN--QVCPICLTNPKDMAFGCGH 420
                RT RS SFQ +      Y+ GV +T  S++H ++  Q CP+CL + K+MAF CGH
Sbjct: 361 MRNSPRTSRSTSFQNKP-----YDNGVSSTPPSTTHNESQQQFCPVCLVSAKNMAFNCGH 420

Query: 421 QTCCDCGETLELCPICRSFIQTRIRLY 433
           QTC  CGE L +CPICRS I  RI+LY
Sbjct: 421 QTCAGCGEDLHVCPICRSSISVRIKLY 433