Tan0008749 (gene) Snake gourd v1

Overview
NameTan0008749
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionPectin lyase-like superfamily protein
LocationLG06: 5263865 .. 5265853 (+)
RNA-Seq ExpressionTan0008749
SyntenyTan0008749
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTAGTTTTGTGTTGTCAATTGCCTTTTGGTTTTCTTTTATTTGTTGCCTTCTTTCTTCTTCTTCTTCTCAAGCTAGATGGCATCATCACACCAAGCAAAAACACTCTCACCACCATAAACATAGCTACATTTCACACCCACCTTCTATTTCACACCCTCCTTCTCCTTCTCCTTCTCCTTCTGATTCACCCGACGATGGAGATTACATCGGCGAAACAAATGTTTTCGACGTGAGAGCTTTCGGTGCCATCGGCAACGGTGTTGCAGACGACACGGAGGCGTTCAAGATGGCATGGGACATGGCATGCCAAAGCGACGACTCTGCAGTCATGCTTGTTCCATATAGTTTCTCATTTATGATTCAATCAACAATCTTCACTGGCCCTTGTCATGGTCACCTCATGTTACGGGTCGATGGCACGTTGATGCCACCGGACGGTCCCGACGCTTGGCCGAAGAATGCTAGTCGCCGACAATGGTTGGTCTTTTATAGAATTGATGGAATGTCCTTAGAAGGAGGAGGGGTGATTGATGGGAGAGGTCAAAAATGGTGGGATCTTCCTTGCAAACCACACAAGGTACTCATTTTTCTTCCATATTGTTTAAGGTAAATTCCTGCAATTTTCATCTCCACTACAAGTTCTAACACAAGATATTTTTGAAACATTAGGGAATAAATGGAACTACGTTACCAGGACCATGCGATAGTCCAATTGTAAGTTCATAAACTTGCTGATCATATTATAAACTTTTTACGTAAGATATATATGAAGAAACTGAGACATATCACATTCTAATGATTTTAGGCTATAAGGTTCTTTATGAGCTCCAATTTGACTGTTCAAGGACTTAAAATCACAAACAGTCCTCAGTTTCACTTCAGATTCGACGGGTGCAAGAATGTTCATATCGACTCGATTCACATAAAATCTCCTGGTCTTAGTCCCAATACAGACGGTATCCACATCGAGAACACAAATGGCGTCGAGATATATAATTCAGTCATTTACAATGGTTAGTAGTAGGTTGAAAGCAAAGAAAGCCTAATTTAATCTGAATTATACATTCTGATTGTTTATCATTTATGTAGGGGATGATTGTGTATCAATTGGGTCAGGTTCATATGATGTAGATATCAGAAATATCACATGTGGACCAAGTCATGGAATCAGGTATTATATTTATTAGCAAATCTAAAATATATATTTGCTGTCAACCTGAGTATAGCGCAATTAGTTAAGACATCTATCTAATAATCTATTTTAATAGCAAAAATTGAAATTTTCACCCCACAGTTAGTATTATATTCCTGAGAAAGGAAAAAAGAAAACATGCCTATACCAAGTTCCATGATGATATTCTCAAACTTTTCCCTTCCTCTTTTGGTCCAGCATTGGAAGCCTCGGCGTTCGCAACTCGAGAGCTTGCGTTACGAACATAACAGTCAGGGACTCGGTGATCAAAGTCTCGGACAACGGGGTTCGGATCAAGACATGGCAAGGTGGCTATGGAGCAGTAAAAGGACTAACATTTAGCAACATTCACATGGACAATGTTAGAAATCCTATCATTATAGATCAATACTACTGCTTAACCAAAGATTGCACAAACCAAACCTCAGCTGTCTCAATATCAGACATTCAGTACACAAATATCAAAGGAACATACGACATTCGAAGCCCGCCTATGCACTTCGCCTGCAGCGACTCCGTCCCGTGTACCAATCTAACGTTGTCGGAAATCGAGCTACTTCCTGCTCAGGGAGATATAGTTTTGGACCCTTTTTGTTGGAATGCTTATGGAGATCTTCAAACACTTACCATTCCCCCTGTCTCATGCTTGCAAGAGGGGCTTCCTTCTTCTATTAACTTAAACAATGACCATGAGTATTGTTGATGGCCTTTTACTTACCATACGTACACATTTATAACAACATGTGCCATCATCATATGTGTATATAAAATAATTACGAATGTATTTGAAAT

mRNA sequence

ATGTCTAGTTTTGTGTTGTCAATTGCCTTTTGGTTTTCTTTTATTTGTTGCCTTCTTTCTTCTTCTTCTTCTCAAGCTAGATGGCATCATCACACCAAGCAAAAACACTCTCACCACCATAAACATAGCTACATTTCACACCCACCTTCTATTTCACACCCTCCTTCTCCTTCTCCTTCTCCTTCTGATTCACCCGACGATGGAGATTACATCGGCGAAACAAATGTTTTCGACGTGAGAGCTTTCGGTGCCATCGGCAACGGTGTTGCAGACGACACGGAGGCGTTCAAGATGGCATGGGACATGGCATGCCAAAGCGACGACTCTGCAGTCATGCTTGTTCCATATAGTTTCTCATTTATGATTCAATCAACAATCTTCACTGGCCCTTGTCATGGTCACCTCATGTTACGGGTCGATGGCACGTTGATGCCACCGGACGGTCCCGACGCTTGGCCGAAGAATGCTAGTCGCCGACAATGGTTGGTCTTTTATAGAATTGATGGAATGTCCTTAGAAGGAGGAGGGGTGATTGATGGGAGAGGTCAAAAATGGTGGGATCTTCCTTGCAAACCACACAAGGGAATAAATGGAACTACGTTACCAGGACCATGCGATAGTCCAATTGCTATAAGGTTCTTTATGAGCTCCAATTTGACTGTTCAAGGACTTAAAATCACAAACAGTCCTCAGTTTCACTTCAGATTCGACGGGTGCAAGAATGTTCATATCGACTCGATTCACATAAAATCTCCTGGTCTTAGTCCCAATACAGACGGTATCCACATCGAGAACACAAATGGCGTCGAGATATATAATTCAGTCATTTACAATGGGGATGATTGTGTATCAATTGGGTCAGGTTCATATGATGTAGATATCAGAAATATCACATGTGGACCAAGTCATGGAATCAGCATTGGAAGCCTCGGCGTTCGCAACTCGAGAGCTTGCGTTACGAACATAACAGTCAGGGACTCGGTGATCAAAGTCTCGGACAACGGGGTTCGGATCAAGACATGGCAAGGTGGCTATGGAGCAGTAAAAGGACTAACATTTAGCAACATTCACATGGACAATGTTAGAAATCCTATCATTATAGATCAATACTACTGCTTAACCAAAGATTGCACAAACCAAACCTCAGCTGTCTCAATATCAGACATTCAGTACACAAATATCAAAGGAACATACGACATTCGAAGCCCGCCTATGCACTTCGCCTGCAGCGACTCCGTCCCGTGTACCAATCTAACGTTGTCGGAAATCGAGCTACTTCCTGCTCAGGGAGATATAGTTTTGGACCCTTTTTGTTGGAATGCTTATGGAGATCTTCAAACACTTACCATTCCCCCTGTCTCATGCTTGCAAGAGGGGCTTCCTTCTTCTATTAACTTAAACAATGACCATGAGTATTGTTGATGGCCTTTTACTTACCATACGTACACATTTATAACAACATGTGCCATCATCATATGTGTATATAAAATAATTACGAATGTATTTGAAAT

Coding sequence (CDS)

ATGTCTAGTTTTGTGTTGTCAATTGCCTTTTGGTTTTCTTTTATTTGTTGCCTTCTTTCTTCTTCTTCTTCTCAAGCTAGATGGCATCATCACACCAAGCAAAAACACTCTCACCACCATAAACATAGCTACATTTCACACCCACCTTCTATTTCACACCCTCCTTCTCCTTCTCCTTCTCCTTCTGATTCACCCGACGATGGAGATTACATCGGCGAAACAAATGTTTTCGACGTGAGAGCTTTCGGTGCCATCGGCAACGGTGTTGCAGACGACACGGAGGCGTTCAAGATGGCATGGGACATGGCATGCCAAAGCGACGACTCTGCAGTCATGCTTGTTCCATATAGTTTCTCATTTATGATTCAATCAACAATCTTCACTGGCCCTTGTCATGGTCACCTCATGTTACGGGTCGATGGCACGTTGATGCCACCGGACGGTCCCGACGCTTGGCCGAAGAATGCTAGTCGCCGACAATGGTTGGTCTTTTATAGAATTGATGGAATGTCCTTAGAAGGAGGAGGGGTGATTGATGGGAGAGGTCAAAAATGGTGGGATCTTCCTTGCAAACCACACAAGGGAATAAATGGAACTACGTTACCAGGACCATGCGATAGTCCAATTGCTATAAGGTTCTTTATGAGCTCCAATTTGACTGTTCAAGGACTTAAAATCACAAACAGTCCTCAGTTTCACTTCAGATTCGACGGGTGCAAGAATGTTCATATCGACTCGATTCACATAAAATCTCCTGGTCTTAGTCCCAATACAGACGGTATCCACATCGAGAACACAAATGGCGTCGAGATATATAATTCAGTCATTTACAATGGGGATGATTGTGTATCAATTGGGTCAGGTTCATATGATGTAGATATCAGAAATATCACATGTGGACCAAGTCATGGAATCAGCATTGGAAGCCTCGGCGTTCGCAACTCGAGAGCTTGCGTTACGAACATAACAGTCAGGGACTCGGTGATCAAAGTCTCGGACAACGGGGTTCGGATCAAGACATGGCAAGGTGGCTATGGAGCAGTAAAAGGACTAACATTTAGCAACATTCACATGGACAATGTTAGAAATCCTATCATTATAGATCAATACTACTGCTTAACCAAAGATTGCACAAACCAAACCTCAGCTGTCTCAATATCAGACATTCAGTACACAAATATCAAAGGAACATACGACATTCGAAGCCCGCCTATGCACTTCGCCTGCAGCGACTCCGTCCCGTGTACCAATCTAACGTTGTCGGAAATCGAGCTACTTCCTGCTCAGGGAGATATAGTTTTGGACCCTTTTTGTTGGAATGCTTATGGAGATCTTCAAACACTTACCATTCCCCCTGTCTCATGCTTGCAAGAGGGGCTTCCTTCTTCTATTAACTTAAACAATGACCATGAGTATTGTTGA

Protein sequence

MSSFVLSIAFWFSFICCLLSSSSSQARWHHHTKQKHSHHHKHSYISHPPSISHPPSPSPSPSDSPDDGDYIGETNVFDVRAFGAIGNGVADDTEAFKMAWDMACQSDDSAVMLVPYSFSFMIQSTIFTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVKGLTFSNIHMDNVRNPIIIDQYYCLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDHEYC
Homology
BLAST of Tan0008749 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 558.5 bits (1438), Expect = 7.1e-158
Identity = 263/444 (59.23%), Postives = 334/444 (75.23%), Query Frame = 0

Query: 24  SQARWHHHTKQKHSHHHKHSYISHPP-SISHPP-----SPSPSPSDSPDDGDYIGETNVF 83
           ++AR+H+H K +H   H       PP   ++PP      P PSP  +P D D      VF
Sbjct: 25  ARARYHYH-KGRHGVTHPLPPPPPPPLETANPPDQVPSDPYPSPDPAPGDSD---SGCVF 84

Query: 84  DVRAFGAIGNGVADDTEAFKMAWDMACQSDDSAVMLVPYSFSFMIQSTIFTGPCHGHLML 143
           DV +FGA+G+G  DDT AF+ AW  AC + +S V+L P    F I STIF+GPC   L+ 
Sbjct: 85  DVTSFGAVGDGSCDDTAAFQDAWKAAC-AVESGVVLAPEGGVFKITSTIFSGPCKPGLVF 144

Query: 144 RVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKGIN 203
           ++DG LMPPDGP+ WP+  ++ QWLVFYR+DG +  G G ++G GQKWWDLPCKPH+G +
Sbjct: 145 QLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWDLPCKPHRGPD 204

Query: 204 GTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLSPN 263
           G++  GPC SP  IRFFMS+N+ V+GL+I NSPQFH +FDGC+ V I+ I I SP LSPN
Sbjct: 205 GSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVLINEIQISSPKLSPN 264

Query: 264 TDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNSRA 323
           TDGIH+ NT  V IYNSV+ NGDDC+SIG+G  DVDI+ +TCGPSHGISIGSLGV NS+A
Sbjct: 265 TDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSHGISIGSLGVHNSQA 324

Query: 324 CVTNITVRDSVIKVSDNGVRIKTWQGGYGAVKGLTFSNIHMDNVRNPIIIDQYYCLTKDC 383
           CV+NITVR++VI+ SDNG+R+KTWQGG G+V  L F NI M+NV N II+DQYYC +KDC
Sbjct: 325 CVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLNCIIVDQYYCQSKDC 384

Query: 384 TNQTSAVSISDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGDIVLDPF 443
            N+TSAV + D++Y NIKGTYD+RSPP+HFACSD+V CTN+T+SE+ELLP +G++V DPF
Sbjct: 385 RNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEVELLPEEGELVDDPF 444

Query: 444 CWNAYGDLQTLTIPPVSCLQEGLP 462
           CWNAYG  +TLTIPP+ CL +G P
Sbjct: 445 CWNAYGKQETLTIPPIDCLLDGSP 463

BLAST of Tan0008749 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 341.7 bits (875), Expect = 1.4e-92
Identity = 168/379 (44.33%), Postives = 230/379 (60.69%), Query Frame = 0

Query: 82  FGAIGNGVADDTEAFKMAWDMACQSDDSAVMLVPYSFSFMIQSTIFTGPCHGHLMLRVDG 141
           FGA GNGV DDT+AF  AW  AC S     +LVP +++ +++    +GPC   L L++ G
Sbjct: 58  FGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCKARLTLQISG 117

Query: 142 TLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKGINGTTL 201
           T++ P+ PD W +  +RR+WL F+ +  +++EGGG ++G GQ+WW+  CK H   N    
Sbjct: 118 TIIAPNDPDVW-EGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCK-HNHSN---- 177

Query: 202 PGPC-DSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLSPNTDG 261
             PC  +P A+ F    N+ V+ L + +S Q H     C+ V I  + + +P  SPNTDG
Sbjct: 178 --PCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDG 237

Query: 262 IHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNSRACVT 321
           IHI  + G+ I N+ +  GDDC+SI   S  + I NI CGP HGISIGSLG   S   V 
Sbjct: 238 IHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVR 297

Query: 322 NITVRDSVIKVSDNGVRIKTWQGGYGAVKGLTFSNIHMDNVRNPIIIDQYYC-LTKDCTN 381
           +ITV  ++I  + NGVRIKTWQGG G V  + F NI M+NV NPIIIDQYYC   K C N
Sbjct: 298 DITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCAN 357

Query: 382 QTSAVSISDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGDIVLDPFCW 441
           QTSA+SI +I + +++GT       +  +CSDS PC N+ L +I+L P+ GD   + FCW
Sbjct: 358 QTSAISIENISFVHVRGT-SASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFTESFCW 417

Query: 442 NAYGDLQTLTIPPVSCLQE 459
            AYG       PP  CL +
Sbjct: 418 EAYGSSSGQVYPP-PCLSD 426

BLAST of Tan0008749 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 8.9e-84
Identity = 158/359 (44.01%), Postives = 217/359 (60.45%), Query Frame = 0

Query: 75  NVFDVRAFGAIGNGVADDTEAFKMAWDMACQSDDSAVMLVPYSFSFMIQSTIFTGPCHGH 134
           N+F+V  +GA+G+G  D TEAF  AW  AC+   SA++LVP +  F++ +  F GPC  H
Sbjct: 57  NIFNVEKYGAVGDGKHDCTEAFSTAWQAACKK-PSAMLLVPGNKKFVVNNLFFNGPCQPH 116

Query: 135 LMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHK 194
              +VDG +     P +W  N   R WL F ++ G +L G GVIDG+G++WW   C   K
Sbjct: 117 FTFKVDGIIAAYQNPASWKNN---RIWLQFAKLTGFTLMGKGVIDGQGKQWWAGQC---K 176

Query: 195 GINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGL 254
            +NG  +    D P AI+F  S+ L +QGLK+ NSP+FH  F  C+ V I  I I +P  
Sbjct: 177 WVNGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAPRD 236

Query: 255 SPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRN 314
           SPNTDGI I  +    +  + I  GDDCV+IG+GS ++ I ++ CGP HGISIGSLG  N
Sbjct: 237 SPNTDGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIGSLGREN 296

Query: 315 SRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVKGLTFSNIHMDNVRNPIIIDQYYCLT 374
           SRA V+ + V  +    + NG+RIKTWQGG G    + + N+ M N  NPI+I+Q+YC +
Sbjct: 297 SRAEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYCTS 356

Query: 375 KD-CTNQTSAVSISDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGDI 433
              C NQ SAV I D+ Y NI+GT    +  +   CSDS+PC ++ LS+I L    G I
Sbjct: 357 ASACQNQRSAVQIQDVTYKNIRGT-SATAAAIQLKCSDSMPCKDIKLSDISLKLTSGKI 407

BLAST of Tan0008749 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 8.3e-82
Identity = 158/356 (44.38%), Postives = 218/356 (61.24%), Query Frame = 0

Query: 76  VFDVRAFGAIGNGVADDTEAFKMAWDMACQSDDSAVMLVPYSFSFMIQSTIFTGPCHGHL 135
           VF+V  +GA+G+G  D TEAF   W+ AC+   SAV+LVP +  F + + +F GPC  HL
Sbjct: 58  VFNVEQYGAVGDGKHDSTEAFATTWNAACKK-ASAVLLVPANKKFFVNNLVFRGPCQPHL 117

Query: 136 MLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKG 195
             +VDGT++    P  W KN+  + WL F ++   +L G GVIDG+GQ+WW   CK    
Sbjct: 118 SFKVDGTIVAQPDPARW-KNS--KIWLQFAQLTDFNLMGTGVIDGQGQQWWAGQCKV--- 177

Query: 196 INGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLS 255
           +NG T+    + P AI+   S ++TV+ L + NSP+FH  F  C+ V I  + IK+P  S
Sbjct: 178 VNGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQGLKIKAPRDS 237

Query: 256 PNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNS 315
           PNTDGI I  +    I   VI  GDDC++IG+GS ++ I+++ CGP HGISIGSLG  NS
Sbjct: 238 PNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGHGISIGSLGRDNS 297

Query: 316 RACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVKGLTFSNIHMDNVRNPIIIDQYYCLTK 375
           RA V+++ V  +    + NG+RIKTWQGG G    +T+ N+ M N  NPI+I+Q+YC + 
Sbjct: 298 RAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCTSA 357

Query: 376 D-CTNQTSAVSISDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQG 431
             C NQ SAV I  + Y NI GT    +  +   CSDSVPCT + LS + L    G
Sbjct: 358 SACQNQRSAVQIQGVTYKNIHGT-SATAAAIQLMCSDSVPCTGIQLSNVSLKLTSG 405

BLAST of Tan0008749 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 4.1e-81
Identity = 160/384 (41.67%), Postives = 225/384 (58.59%), Query Frame = 0

Query: 78  DVRAFGAIGNGVADDTEAFKMAWDMACQSDDSAVMLVPYSFSFMIQSTIFTGPCHGHLML 137
           +V  FGA G+G  DDT+AF+ AW  AC S  SAV+LVP   +++++   F+GPC   L +
Sbjct: 91  NVDDFGAKGDG-RDDTKAFEKAWKAACSSTSSAVLLVPKK-NYLVRPISFSGPCKSGLTM 150

Query: 138 RVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKGIN 197
           ++ GT+   D    + K+   R WLVF  +  + +EGGG I+G G+ WW   CK +K + 
Sbjct: 151 QIYGTIEASDDRSDYRKDG--RHWLVFDSVQNLRVEGGGTINGNGKIWWQNSCKTNKAL- 210

Query: 198 GTTLPGPC-DSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLSP 257
                 PC D+P A+ F+ S ++ V+ LKI N+ Q H  FD C NV   ++ + +P  SP
Sbjct: 211 ------PCKDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNLMVTAPENSP 270

Query: 258 NTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNSR 317
           NTDGIH+  T  + I + VI  GDDC+SI +GS  V + +ITCGP HGISIGSLG  NS 
Sbjct: 271 NTDGIHVTGTQNIHISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGISIGSLGYGNSE 330

Query: 318 ACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVKGLTFSNIHMDNVRNPIIIDQYYC-LTK 377
           A V+++ V  + +  + NGVRIKTWQGG G+   + F N+ M NV NPIIIDQ YC   K
Sbjct: 331 AHVSDVVVNGAKLCGTTNGVRIKTWQGGSGSASNIKFQNVEMHNVENPIIIDQNYCDQDK 390

Query: 378 DCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGDIVLD 437
            C  Q+SAV + ++ Y NIKGT    +  + F CS   PC  + L +++ L  +G     
Sbjct: 391 PCQEQSSAVQVKNVVYQNIKGTC-ASNVAITFDCSKRFPCQGIVLEDVD-LEIEGGAAAK 450

Query: 438 PFCWNAYGDLQTLTIPPVSCLQEG 460
             C N   +L    +    C +EG
Sbjct: 451 ALCNNV--ELSETGVVSPHCQEEG 459

BLAST of Tan0008749 vs. NCBI nr
Match: XP_038899064.1 (polygalacturonase At1g48100-like [Benincasa hispida])

HSP 1 Score: 936.8 bits (2420), Expect = 7.3e-269
Identity = 447/474 (94.30%), Postives = 458/474 (96.62%), Query Frame = 0

Query: 1   MSSFVLSIAFWFSFICCLLSSSSSQARWHHHTKQKHSHHHKHSYISHPPSISHPPSPSPS 60
           MSSFVLS A WFSFI CLL SSSSQARWHHHTKQKHSHHHKHSYISHPPS+    SPSPS
Sbjct: 1   MSSFVLSFAIWFSFISCLL-SSSSQARWHHHTKQKHSHHHKHSYISHPPSL----SPSPS 60

Query: 61  PSDS-PDDGDYIGETNVFDVRAFGAIGNGVADDTEAFKMAWDMACQSDDSAVMLVPYSFS 120
           PSDS PDDGDYI ETN+FDVRAFGAIGNGV DDTEAFKMAWD ACQSDDSA+MLVPY +S
Sbjct: 61  PSDSPPDDGDYINETNIFDVRAFGAIGNGVVDDTEAFKMAWDTACQSDDSAIMLVPYGYS 120

Query: 121 FMIQSTIFTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVID 180
           FMIQSTIFTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVID
Sbjct: 121 FMIQSTIFTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVID 180

Query: 181 GRGQKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGC 240
           GRGQKWWDLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGC
Sbjct: 181 GRGQKWWDLPCKPHKGRNGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGC 240

Query: 241 KNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITC 300
           KNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYD+DIRNITC
Sbjct: 241 KNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDLDIRNITC 300

Query: 301 GPSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVKGLTFSNIHMD 360
           GPSHGISIGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAV+G+TFSNIHMD
Sbjct: 301 GPSHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMD 360

Query: 361 NVRNPIIIDQYYCLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLT 420
           NVRNPIIIDQ+YCLTKDC NQTSAVS+SDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLT
Sbjct: 361 NVRNPIIIDQFYCLTKDCINQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLT 420

Query: 421 LSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDHEYC 474
           LSEIELLP+QGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNND+EYC
Sbjct: 421 LSEIELLPSQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDNEYC 469

BLAST of Tan0008749 vs. NCBI nr
Match: TYJ96898.1 (polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 911.0 bits (2353), Expect = 4.3e-261
Identity = 436/475 (91.79%), Postives = 451/475 (94.95%), Query Frame = 0

Query: 1   MSSFV-LSIAFWFSFICCLLSSSSSQARWHHHTKQKHSHHHKHSYISHPPSISHPPSPSP 60
           MSSFV LS A W SF+ CLL  SSSQARWHHHTKQKHSHHH+HSYISHPPS+      SP
Sbjct: 1   MSSFVLLSFAIWLSFVSCLL--SSSQARWHHHTKQKHSHHHRHSYISHPPSL------SP 60

Query: 61  SPSDSPDDGDYIGETNVFDVRAFGAIGNGVADDTEAFKMAWDMACQS-DDSAVMLVPYSF 120
           SPSDSPDDGDYI +TN+FDVRAFGAIG+G+ DDTEAFKMAWD ACQS DDSAVMLVPY F
Sbjct: 61  SPSDSPDDGDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGF 120

Query: 121 SFMIQSTIFTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVI 180
           SFMIQSTIFTGPCHG+LMLRVDGTLMPPDGPDAWPKN+SRRQWLVFYRIDGMSLEGGGVI
Sbjct: 121 SFMIQSTIFTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVI 180

Query: 181 DGRGQKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDG 240
           DGRGQKWWDLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDG
Sbjct: 181 DGRGQKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDG 240

Query: 241 CKNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNIT 300
           CK VHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVI NGDDCVSIGSGSYD+DIRNIT
Sbjct: 241 CKTVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNIT 300

Query: 301 CGPSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVKGLTFSNIHM 360
           CGPSHGISIGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAV+G+TFSNIHM
Sbjct: 301 CGPSHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHM 360

Query: 361 DNVRNPIIIDQYYCLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFACSDSVPCTNL 420
           DNVRNPIIIDQ+YCLTKDCTNQTSAVS+SDIQYTNIKGTYDIRSPPMHFACSDSVPCTNL
Sbjct: 361 DNVRNPIIIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNL 420

Query: 421 TLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDHEYC 474
           TLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIN NND+EYC
Sbjct: 421 TLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

BLAST of Tan0008749 vs. NCBI nr
Match: XP_008461394.1 (PREDICTED: polygalacturonase At1g48100-like [Cucumis melo])

HSP 1 Score: 909.4 bits (2349), Expect = 1.3e-260
Identity = 435/475 (91.58%), Postives = 450/475 (94.74%), Query Frame = 0

Query: 1   MSSFV-LSIAFWFSFICCLLSSSSSQARWHHHTKQKHSHHHKHSYISHPPSISHPPSPSP 60
           MSSFV LS A W SF+ CLL  SSSQARWHHHTKQKHSHHH+H YISHPPS+      SP
Sbjct: 1   MSSFVLLSFAIWLSFVSCLL--SSSQARWHHHTKQKHSHHHRHGYISHPPSL------SP 60

Query: 61  SPSDSPDDGDYIGETNVFDVRAFGAIGNGVADDTEAFKMAWDMACQS-DDSAVMLVPYSF 120
           SPSDSPDDGDYI +TN+FDVRAFGAIG+G+ DDTEAFKMAWD ACQS DDSAVMLVPY F
Sbjct: 61  SPSDSPDDGDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGF 120

Query: 121 SFMIQSTIFTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVI 180
           SFMIQSTIFTGPCHG+LMLRVDGTLMPPDGPDAWPKN+SRRQWLVFYRIDGMSLEGGGVI
Sbjct: 121 SFMIQSTIFTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVI 180

Query: 181 DGRGQKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDG 240
           DGRGQKWWDLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDG
Sbjct: 181 DGRGQKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDG 240

Query: 241 CKNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNIT 300
           CK VHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVI NGDDCVSIGSGSYD+DIRNIT
Sbjct: 241 CKTVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNIT 300

Query: 301 CGPSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVKGLTFSNIHM 360
           CGPSHGISIGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAV+G+TFSNIHM
Sbjct: 301 CGPSHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHM 360

Query: 361 DNVRNPIIIDQYYCLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFACSDSVPCTNL 420
           DNVRNPIIIDQ+YCLTKDCTNQTSAVS+SDIQYTNIKGTYDIRSPPMHFACSDSVPCTNL
Sbjct: 361 DNVRNPIIIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNL 420

Query: 421 TLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDHEYC 474
           TLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIN NND+EYC
Sbjct: 421 TLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

BLAST of Tan0008749 vs. NCBI nr
Match: XP_022146163.1 (polygalacturonase At1g48100 [Momordica charantia])

HSP 1 Score: 904.8 bits (2337), Expect = 3.1e-259
Identity = 428/473 (90.49%), Postives = 446/473 (94.29%), Query Frame = 0

Query: 1   MSSFVLSIAFWFSFICCLLSSSSSQARWHHHTKQKHSHHHKHSYISHPPSISHPPSPSPS 60
           M+SFVLS+AFW SFICCL+   SSQ RWHH  KQKHSHHHKHSYIS PPS      P P 
Sbjct: 1   MASFVLSLAFWCSFICCLV---SSQGRWHHPPKQKHSHHHKHSYISQPPS-----PPPPP 60

Query: 61  PSDSPDDGDYIGETNVFDVRAFGAIGNGVADDTEAFKMAWDMACQSDDSAVMLVPYSFSF 120
           P DSPD+GDY+ E +VFDVRAFGAIG+G ADDT AFKMAWD ACQSDD AV+LVPYSFSF
Sbjct: 61  PPDSPDEGDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSF 120

Query: 121 MIQSTIFTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDG 180
           MIQSTIFTGPC GHLMLRVDGTLMPPDGPDAWPKN S RQWLVFYRIDGMSLEGGGVIDG
Sbjct: 121 MIQSTIFTGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDG 180

Query: 181 RGQKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCK 240
           RGQKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCK
Sbjct: 181 RGQKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCK 240

Query: 241 NVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCG 300
           NVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCG
Sbjct: 241 NVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCG 300

Query: 301 PSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVKGLTFSNIHMDN 360
           PSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAV+G+TFSN+HMDN
Sbjct: 301 PSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNVHMDN 360

Query: 361 VRNPIIIDQYYCLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTL 420
           VRNPIIIDQ+YCL+KDCTNQTSAVS+SDIQY+NIKGTYDIRSPPMH ACSDSVPCTNLTL
Sbjct: 361 VRNPIIIDQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTL 420

Query: 421 SEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDHEYC 474
           SEIELLPAQGDIVLDP+CWNAYGDLQTLTIPP+SCLQEGLPSSINLNND+EYC
Sbjct: 421 SEIELLPAQGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 465

BLAST of Tan0008749 vs. NCBI nr
Match: XP_004135975.1 (polygalacturonase At1g48100 [Cucumis sativus] >KGN45046.1 hypothetical protein Csa_016395 [Cucumis sativus])

HSP 1 Score: 899.4 bits (2323), Expect = 1.3e-257
Identity = 431/475 (90.74%), Postives = 447/475 (94.11%), Query Frame = 0

Query: 1   MSSFV-LSIAFWFSFICCLLSSSSSQARWHHHTKQKHSHHHKHSYISHPPSISHPPSPSP 60
           MSSFV LS A W SF+ CLL  SSSQARWHHHTKQKHSH H+HSYISHPPS+      SP
Sbjct: 1   MSSFVLLSFAIWLSFVSCLL--SSSQARWHHHTKQKHSHRHRHSYISHPPSL------SP 60

Query: 61  SPSDSPDDGDYIGETNVFDVRAFGAIGNGVADDTEAFKMAWDMACQS-DDSAVMLVPYSF 120
           SPSDSPDDG YI +TN+FDVRAFGAIG+G+ DDTEAFKMAWD ACQS DDS VMLVPY F
Sbjct: 61  SPSDSPDDGYYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGF 120

Query: 121 SFMIQSTIFTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVI 180
           SFMIQSTIFTGPCHG+LMLRVDGTLM PDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVI
Sbjct: 121 SFMIQSTIFTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVI 180

Query: 181 DGRGQKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDG 240
           DGRGQKWWDLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDG
Sbjct: 181 DGRGQKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDG 240

Query: 241 CKNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNIT 300
           CK VHIDSIHIKSPGLSPNTDGIHIENTNGV+IYNSVI NGDDCVSIGSGSYD+DIRNIT
Sbjct: 241 CKTVHIDSIHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNIT 300

Query: 301 CGPSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVKGLTFSNIHM 360
           CGPSHGISIGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAV+G+TFSNIHM
Sbjct: 301 CGPSHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHM 360

Query: 361 DNVRNPIIIDQYYCLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFACSDSVPCTNL 420
           DNVRNPIIIDQ+YCLTKDCTNQTSAVS+SDIQYTNIKGTYDIRSPPMHFACSDSVPCTNL
Sbjct: 361 DNVRNPIIIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNL 420

Query: 421 TLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDHEYC 474
           TLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSS+N NND+EYC
Sbjct: 421 TLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSVNFNNDNEYC 467

BLAST of Tan0008749 vs. ExPASy TrEMBL
Match: A0A5D3BFC7 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold670G00080 PE=3 SV=1)

HSP 1 Score: 911.0 bits (2353), Expect = 2.1e-261
Identity = 436/475 (91.79%), Postives = 451/475 (94.95%), Query Frame = 0

Query: 1   MSSFV-LSIAFWFSFICCLLSSSSSQARWHHHTKQKHSHHHKHSYISHPPSISHPPSPSP 60
           MSSFV LS A W SF+ CLL  SSSQARWHHHTKQKHSHHH+HSYISHPPS+      SP
Sbjct: 1   MSSFVLLSFAIWLSFVSCLL--SSSQARWHHHTKQKHSHHHRHSYISHPPSL------SP 60

Query: 61  SPSDSPDDGDYIGETNVFDVRAFGAIGNGVADDTEAFKMAWDMACQS-DDSAVMLVPYSF 120
           SPSDSPDDGDYI +TN+FDVRAFGAIG+G+ DDTEAFKMAWD ACQS DDSAVMLVPY F
Sbjct: 61  SPSDSPDDGDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGF 120

Query: 121 SFMIQSTIFTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVI 180
           SFMIQSTIFTGPCHG+LMLRVDGTLMPPDGPDAWPKN+SRRQWLVFYRIDGMSLEGGGVI
Sbjct: 121 SFMIQSTIFTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVI 180

Query: 181 DGRGQKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDG 240
           DGRGQKWWDLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDG
Sbjct: 181 DGRGQKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDG 240

Query: 241 CKNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNIT 300
           CK VHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVI NGDDCVSIGSGSYD+DIRNIT
Sbjct: 241 CKTVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNIT 300

Query: 301 CGPSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVKGLTFSNIHM 360
           CGPSHGISIGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAV+G+TFSNIHM
Sbjct: 301 CGPSHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHM 360

Query: 361 DNVRNPIIIDQYYCLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFACSDSVPCTNL 420
           DNVRNPIIIDQ+YCLTKDCTNQTSAVS+SDIQYTNIKGTYDIRSPPMHFACSDSVPCTNL
Sbjct: 361 DNVRNPIIIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNL 420

Query: 421 TLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDHEYC 474
           TLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIN NND+EYC
Sbjct: 421 TLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

BLAST of Tan0008749 vs. ExPASy TrEMBL
Match: A0A1S3CFX8 (polygalacturonase At1g48100-like OS=Cucumis melo OX=3656 GN=LOC103499990 PE=3 SV=1)

HSP 1 Score: 909.4 bits (2349), Expect = 6.1e-261
Identity = 435/475 (91.58%), Postives = 450/475 (94.74%), Query Frame = 0

Query: 1   MSSFV-LSIAFWFSFICCLLSSSSSQARWHHHTKQKHSHHHKHSYISHPPSISHPPSPSP 60
           MSSFV LS A W SF+ CLL  SSSQARWHHHTKQKHSHHH+H YISHPPS+      SP
Sbjct: 1   MSSFVLLSFAIWLSFVSCLL--SSSQARWHHHTKQKHSHHHRHGYISHPPSL------SP 60

Query: 61  SPSDSPDDGDYIGETNVFDVRAFGAIGNGVADDTEAFKMAWDMACQS-DDSAVMLVPYSF 120
           SPSDSPDDGDYI +TN+FDVRAFGAIG+G+ DDTEAFKMAWD ACQS DDSAVMLVPY F
Sbjct: 61  SPSDSPDDGDYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSAVMLVPYGF 120

Query: 121 SFMIQSTIFTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVI 180
           SFMIQSTIFTGPCHG+LMLRVDGTLMPPDGPDAWPKN+SRRQWLVFYRIDGMSLEGGGVI
Sbjct: 121 SFMIQSTIFTGPCHGYLMLRVDGTLMPPDGPDAWPKNSSRRQWLVFYRIDGMSLEGGGVI 180

Query: 181 DGRGQKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDG 240
           DGRGQKWWDLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDG
Sbjct: 181 DGRGQKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDG 240

Query: 241 CKNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNIT 300
           CK VHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVI NGDDCVSIGSGSYD+DIRNIT
Sbjct: 241 CKTVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVINNGDDCVSIGSGSYDLDIRNIT 300

Query: 301 CGPSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVKGLTFSNIHM 360
           CGPSHGISIGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAV+G+TFSNIHM
Sbjct: 301 CGPSHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHM 360

Query: 361 DNVRNPIIIDQYYCLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFACSDSVPCTNL 420
           DNVRNPIIIDQ+YCLTKDCTNQTSAVS+SDIQYTNIKGTYDIRSPPMHFACSDSVPCTNL
Sbjct: 361 DNVRNPIIIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNL 420

Query: 421 TLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDHEYC 474
           TLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIN NND+EYC
Sbjct: 421 TLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINFNNDNEYC 467

BLAST of Tan0008749 vs. ExPASy TrEMBL
Match: A0A6J1CXU8 (polygalacturonase At1g48100 OS=Momordica charantia OX=3673 GN=LOC111015443 PE=3 SV=1)

HSP 1 Score: 904.8 bits (2337), Expect = 1.5e-259
Identity = 428/473 (90.49%), Postives = 446/473 (94.29%), Query Frame = 0

Query: 1   MSSFVLSIAFWFSFICCLLSSSSSQARWHHHTKQKHSHHHKHSYISHPPSISHPPSPSPS 60
           M+SFVLS+AFW SFICCL+   SSQ RWHH  KQKHSHHHKHSYIS PPS      P P 
Sbjct: 1   MASFVLSLAFWCSFICCLV---SSQGRWHHPPKQKHSHHHKHSYISQPPS-----PPPPP 60

Query: 61  PSDSPDDGDYIGETNVFDVRAFGAIGNGVADDTEAFKMAWDMACQSDDSAVMLVPYSFSF 120
           P DSPD+GDY+ E +VFDVRAFGAIG+G ADDT AFKMAWD ACQSDD AV+LVPYSFSF
Sbjct: 61  PPDSPDEGDYVSEMDVFDVRAFGAIGDGTADDTAAFKMAWDTACQSDDPAVILVPYSFSF 120

Query: 121 MIQSTIFTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDG 180
           MIQSTIFTGPC GHLMLRVDGTLMPPDGPDAWPKN S RQWLVFYRIDGMSLEGGGVIDG
Sbjct: 121 MIQSTIFTGPCQGHLMLRVDGTLMPPDGPDAWPKNTSHRQWLVFYRIDGMSLEGGGVIDG 180

Query: 181 RGQKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCK 240
           RGQKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCK
Sbjct: 181 RGQKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCK 240

Query: 241 NVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCG 300
           NVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCG
Sbjct: 241 NVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCG 300

Query: 301 PSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVKGLTFSNIHMDN 360
           PSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAV+G+TFSN+HMDN
Sbjct: 301 PSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVRGITFSNVHMDN 360

Query: 361 VRNPIIIDQYYCLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTL 420
           VRNPIIIDQ+YCL+KDCTNQTSAVS+SDIQY+NIKGTYDIRSPPMH ACSDSVPCTNLTL
Sbjct: 361 VRNPIIIDQFYCLSKDCTNQTSAVSVSDIQYSNIKGTYDIRSPPMHLACSDSVPCTNLTL 420

Query: 421 SEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDHEYC 474
           SEIELLPAQGDIVLDP+CWNAYGDLQTLTIPP+SCLQEGLPSSINLNND+EYC
Sbjct: 421 SEIELLPAQGDIVLDPYCWNAYGDLQTLTIPPISCLQEGLPSSINLNNDNEYC 465

BLAST of Tan0008749 vs. ExPASy TrEMBL
Match: A0A0A0KBA7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G414430 PE=3 SV=1)

HSP 1 Score: 899.4 bits (2323), Expect = 6.3e-258
Identity = 431/475 (90.74%), Postives = 447/475 (94.11%), Query Frame = 0

Query: 1   MSSFV-LSIAFWFSFICCLLSSSSSQARWHHHTKQKHSHHHKHSYISHPPSISHPPSPSP 60
           MSSFV LS A W SF+ CLL  SSSQARWHHHTKQKHSH H+HSYISHPPS+      SP
Sbjct: 1   MSSFVLLSFAIWLSFVSCLL--SSSQARWHHHTKQKHSHRHRHSYISHPPSL------SP 60

Query: 61  SPSDSPDDGDYIGETNVFDVRAFGAIGNGVADDTEAFKMAWDMACQS-DDSAVMLVPYSF 120
           SPSDSPDDG YI +TN+FDVRAFGAIG+G+ DDTEAFKMAWD ACQS DDS VMLVPY F
Sbjct: 61  SPSDSPDDGYYINDTNIFDVRAFGAIGDGIVDDTEAFKMAWDTACQSEDDSVVMLVPYGF 120

Query: 121 SFMIQSTIFTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVI 180
           SFMIQSTIFTGPCHG+LMLRVDGTLM PDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVI
Sbjct: 121 SFMIQSTIFTGPCHGYLMLRVDGTLMTPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVI 180

Query: 181 DGRGQKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDG 240
           DGRGQKWWDLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTV GLKITNSPQFHFRFDG
Sbjct: 181 DGRGQKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVHGLKITNSPQFHFRFDG 240

Query: 241 CKNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNIT 300
           CK VHIDSIHIKSPGLSPNTDGIHIENTNGV+IYNSVI NGDDCVSIGSGSYD+DIRNIT
Sbjct: 241 CKTVHIDSIHIKSPGLSPNTDGIHIENTNGVQIYNSVINNGDDCVSIGSGSYDLDIRNIT 300

Query: 301 CGPSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVKGLTFSNIHM 360
           CGPSHGISIGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAV+G+TFSNIHM
Sbjct: 301 CGPSHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHM 360

Query: 361 DNVRNPIIIDQYYCLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFACSDSVPCTNL 420
           DNVRNPIIIDQ+YCLTKDCTNQTSAVS+SDIQYTNIKGTYDIRSPPMHFACSDSVPCTNL
Sbjct: 361 DNVRNPIIIDQFYCLTKDCTNQTSAVSVSDIQYTNIKGTYDIRSPPMHFACSDSVPCTNL 420

Query: 421 TLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDHEYC 474
           TLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSS+N NND+EYC
Sbjct: 421 TLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSVNFNNDNEYC 467

BLAST of Tan0008749 vs. ExPASy TrEMBL
Match: A0A6J1GP58 (polygalacturonase At1g48100 OS=Cucurbita moschata OX=3662 GN=LOC111456199 PE=3 SV=1)

HSP 1 Score: 895.2 bits (2312), Expect = 1.2e-256
Identity = 422/473 (89.22%), Postives = 444/473 (93.87%), Query Frame = 0

Query: 1   MSSFVLSIAFWFSFICCLLSSSSSQARWHHHTKQKHSHHHKHSYISHPPSISHPPSPSPS 60
           MS+FVLS++FW +F+CCLL   SSQARWHHH+KQKHSHHHK SYISHP          P+
Sbjct: 1   MSNFVLSLSFWVTFLCCLL--CSSQARWHHHSKQKHSHHHKRSYISHP----------PT 60

Query: 61  PSDSPDDGDYIGETNVFDVRAFGAIGNGVADDTEAFKMAWDMACQSDDSAVMLVPYSFSF 120
           PS SP DGDYI ETN+FDVR FGA+G+GVADDTEAFKMAWDMACQSDD+AV+LVPY +SF
Sbjct: 61  PSPSPGDGDYINETNIFDVRGFGAVGDGVADDTEAFKMAWDMACQSDDAAVVLVPYGYSF 120

Query: 121 MIQSTIFTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDG 180
           MIQSTIFTGPCHGHLML VDGTLMPPDGPDAWP+NASRRQWLVFYRIDGMSLEGGGV+DG
Sbjct: 121 MIQSTIFTGPCHGHLMLWVDGTLMPPDGPDAWPRNASRRQWLVFYRIDGMSLEGGGVVDG 180

Query: 181 RGQKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCK 240
           RGQKWWDLPCKPHKG NGTTLPGPCDSPIAIRFFMSSNLTVQGLK TNSPQFHFRFDGCK
Sbjct: 181 RGQKWWDLPCKPHKGPNGTTLPGPCDSPIAIRFFMSSNLTVQGLKFTNSPQFHFRFDGCK 240

Query: 241 NVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCG 300
           NVHI+SIHIKSPG SPNTDGIHIENTNGV IYNSVIYNGDDCVSIGSGSYDVDIRNITCG
Sbjct: 241 NVHIESIHIKSPGFSPNTDGIHIENTNGVGIYNSVIYNGDDCVSIGSGSYDVDIRNITCG 300

Query: 301 PSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVKGLTFSNIHMDN 360
           PSHGISIGSLGVRNSRACVTNITVRDSVIK SDNGVRIKTWQGGYGAV+G+TFSNIHMDN
Sbjct: 301 PSHGISIGSLGVRNSRACVTNITVRDSVIKDSDNGVRIKTWQGGYGAVRGITFSNIHMDN 360

Query: 361 VRNPIIIDQYYCLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTL 420
           VRNPIIIDQ+YCLTKDCTNQTSAVSISDI+YTNIKGTYDIRS PMH ACSD+VPCTNLTL
Sbjct: 361 VRNPIIIDQFYCLTKDCTNQTSAVSISDIEYTNIKGTYDIRSSPMHLACSDAVPCTNLTL 420

Query: 421 SEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDHEYC 474
           SEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSI LNND+EYC
Sbjct: 421 SEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSIKLNNDNEYC 461

BLAST of Tan0008749 vs. TAIR 10
Match: AT1G02460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 654.1 bits (1686), Expect = 8.8e-188
Identity = 309/458 (67.47%), Postives = 371/458 (81.00%), Query Frame = 0

Query: 22  SSSQARWHHH-TKQKHSHHHKHSYISHPP--SISHPPSPSPSPSD--------SPDDGDY 81
           S S+AR HHH  K KH++H+ HS    PP  SIS PP+P P P D        SP D   
Sbjct: 23  SLSEARNHHHKEKHKHNNHNHHSSKPKPPSSSISQPPTPPPGPPDSPAPSLPPSPSDDPA 82

Query: 82  IGETNVFDVRAFGAIGNGVADDTEAFKMAWDMACQSDDS--AVMLVPYSFSFMIQSTIFT 141
                +++VR +GA+G+G  DDTEAFK AWD +C ++++  +V+LVPY ++FMIQSTIFT
Sbjct: 83  DDNNGIYNVRKYGAVGDGETDDTEAFKTAWDSSCNNENNTDSVLLVPYGYTFMIQSTIFT 142

Query: 142 GPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDL 201
           GPC  +   +VDGT++ PDGP++WP N S+RQWLVFYR++GM+L+G GVIDGRGQKWWDL
Sbjct: 143 GPCRSYQFFQVDGTIVTPDGPESWPSNISKRQWLVFYRVNGMALKGEGVIDGRGQKWWDL 202

Query: 202 PCKPHKGINGTTL-PGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSI 261
           PCKPH+ +N + +  GPCDSPIA+RFFMSSNL V+GL+I NSPQFHFRFDGC+ VH++S+
Sbjct: 203 PCKPHRSVNKSAIVTGPCDSPIALRFFMSSNLRVEGLQIKNSPQFHFRFDGCQGVHVESL 262

Query: 262 HIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGP-SHGIS 321
           HI +P LSPNTDGIHIEN+N V IYNS+I NGDDCVSIGSGSYDVDIRN+TCGP  HGIS
Sbjct: 263 HITAPPLSPNTDGIHIENSNSVTIYNSIISNGDDCVSIGSGSYDVDIRNLTCGPGGHGIS 322

Query: 322 IGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVKGLTFSNIHMDNVRNPII 381
           IGSLG  NSRACV+NITVRDSVIK SDNGVRIKTWQGG G+V G+TF+NIH+D+VRNPII
Sbjct: 323 IGSLGNHNSRACVSNITVRDSVIKYSDNGVRIKTWQGGSGSVSGVTFNNIHVDSVRNPII 382

Query: 382 IDQYYCLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELL 441
           IDQYYC+TKDC N+TSAV +SDI Y  IKGTYDIRSPPMHF CSD+VPCTNLTLS+IELL
Sbjct: 383 IDQYYCMTKDCANKTSAVFVSDIAYQGIKGTYDIRSPPMHFGCSDAVPCTNLTLSDIELL 442

Query: 442 PAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSI 465
           PA+G+IVLDPFCWNAYG  + L+IPPV CL    P  +
Sbjct: 443 PAKGEIVLDPFCWNAYGIAEELSIPPVWCLMSDPPKGL 480

BLAST of Tan0008749 vs. TAIR 10
Match: AT4G01890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 589.3 bits (1518), Expect = 2.7e-168
Identity = 284/468 (60.68%), Postives = 347/468 (74.15%), Query Frame = 0

Query: 5   VLSIAFWFSFICCLLSSSSSQARWHHHTKQKHSHHHKHSYISHPPSISHPPSPSPSPSD- 64
           +L I      I   L S  ++AR HH   Q                   PPSPSP+P+D 
Sbjct: 9   ILCITTLILIITFSLLSYGTEARLHHQASQ-------------------PPSPSPNPNDP 68

Query: 65  --SPDDGDYIGETNVFDVRAFGAIGNGVADDTEAFKMAWDMAC---QSDDSAVMLVPYSF 124
             SP     +    V+DVR +GA+GNGVADDT +FK AWD AC   +++ ++V+ VPY F
Sbjct: 69  SKSPSRSQDLDHEVVYDVRKYGAVGNGVADDTVSFKTAWDSACSNNKNNTASVLHVPYGF 128

Query: 125 SFMIQSTIFTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVI 184
           +FMI+STIFTGPC  +   +VDGT++P DGP +WP + ++RQWL FYRI+GM+L+G GVI
Sbjct: 129 TFMIRSTIFTGPCRSYQYFQVDGTIVPRDGPKSWPSSLNKRQWLAFYRINGMALQGAGVI 188

Query: 185 DGRGQKWWDLPCKPH-KGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFD 244
           DGRGQ WWDLPCKPH + +N T L GPC+SP A+RFFMSSN+ V+GL I NSPQ H + D
Sbjct: 189 DGRGQNWWDLPCKPHQQNVNKTKLAGPCESPAALRFFMSSNVIVKGLSIKNSPQVHLKLD 248

Query: 245 GCKNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNI 304
           GC  VHI+S+ I SP  SPNTDGIHIEN+N VEIYNSVI NGDDCVSIG G+YD+DIRNI
Sbjct: 249 GCHVVHINSLRIISPPASPNTDGIHIENSNSVEIYNSVISNGDDCVSIGPGAYDIDIRNI 308

Query: 305 TCGP-SHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVKGLTFSNI 364
           TCGP  HGISIGSLG +NS ACV+N+TVRDS IK S+NGVRIKTWQGG G+V G+TF NI
Sbjct: 309 TCGPGGHGISIGSLGEKNSHACVSNVTVRDSFIKFSENGVRIKTWQGGSGSVSGVTFDNI 368

Query: 365 HMDNVRNPIIIDQYYCLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFACSDSVPCT 424
           H+D VRNPIIIDQYYC TK C N+TSAV ++DI Y +IKGTYDIRSPPMHF CS++VPCT
Sbjct: 369 HVDTVRNPIIIDQYYCTTKSCANKTSAVFVNDIVYQSIKGTYDIRSPPMHFGCSNNVPCT 428

Query: 425 NLTLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSI 465
           NLTLS IELLP++ DIV+ PFCWNAYG     ++P +SCL+    +S+
Sbjct: 429 NLTLSNIELLPSKEDIVVGPFCWNAYGITDEFSVPLISCLKSNPSTSL 457

BLAST of Tan0008749 vs. TAIR 10
Match: AT1G56710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 563.1 bits (1450), Expect = 2.0e-160
Identity = 258/408 (63.24%), Postives = 327/408 (80.15%), Query Frame = 0

Query: 56  SPSPSPSDSPDDGDYIGETNVFDVRAFGAIGNGVADDTEAFKMAWDMACQS--DDSAVML 115
           SPSP+P+ + +D D I  T VFDV +FGAIG+   DDT AFKMAWD AC S    SA++L
Sbjct: 28  SPSPAPNPAYNDNDNIAPT-VFDVTSFGAIGDCSTDDTSAFKMAWDAACMSTGPKSALLL 87

Query: 116 VPYSFSFMIQSTIFTGPCHGHLMLRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLE 175
           VPY+F F+++ T F GPC  +L+L++DG ++ PDGP +WP N  +RQW++FYR++G+S++
Sbjct: 88  VPYTFCFLVKPTTFNGPCRTNLVLQIDGFIVSPDGPRSWPSN-YQRQWMMFYRVNGLSIQ 147

Query: 176 GGGVIDGRGQKWWDLPCKPHKGINGTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFH 235
           G GVI+GRGQKWW+LPCKPHKG+NGTT  GPCDSP+AIR F SS + +QG+   NS QFH
Sbjct: 148 GSGVINGRGQKWWNLPCKPHKGLNGTTQTGPCDSPVAIRLFQSSKVRIQGINFMNSAQFH 207

Query: 236 FRFDGCKNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVD 295
            RFD C +V +DS+ IK+P  SPNTDGIHIENT+ V+I NS+I NGDDC+SIG+G ++VD
Sbjct: 208 VRFDNCSDVVVDSVIIKAPASSPNTDGIHIENTHNVQIRNSMISNGDDCISIGAGCFNVD 267

Query: 296 IRNITCGPSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVKGLTF 355
           I+N+TCGPSHGISIGSLGV NS+A V+NITV +S I  SDNGVRIKTWQGG G+V  + F
Sbjct: 268 IKNVTCGPSHGISIGSLGVHNSQAYVSNITVTNSTIWNSDNGVRIKTWQGGSGSVSRIVF 327

Query: 356 SNIHMDNVRNPIIIDQYYCLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFACSDSV 415
           SNI M NVRNPI+IDQYYC T +C NQTSAV ISD+ Y NIKGTYD+RSPP+HF CSDSV
Sbjct: 328 SNILMVNVRNPIMIDQYYCQTNNCANQTSAVIISDVLYANIKGTYDLRSPPIHFGCSDSV 387

Query: 416 PCTNLTLSEIELLPAQGDIVLDPFCWNAYGDLQTLTIPPVSCLQEGLP 462
           PCTNLTL+E++L P++G  + +PFCWNAYG ++ +T+PPV CL +  P
Sbjct: 388 PCTNLTLTEVDLFPSKGQHLENPFCWNAYGSMK-ITVPPVYCLLDAPP 432

BLAST of Tan0008749 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 558.5 bits (1438), Expect = 5.0e-159
Identity = 263/444 (59.23%), Postives = 334/444 (75.23%), Query Frame = 0

Query: 24  SQARWHHHTKQKHSHHHKHSYISHPP-SISHPP-----SPSPSPSDSPDDGDYIGETNVF 83
           ++AR+H+H K +H   H       PP   ++PP      P PSP  +P D D      VF
Sbjct: 25  ARARYHYH-KGRHGVTHPLPPPPPPPLETANPPDQVPSDPYPSPDPAPGDSD---SGCVF 84

Query: 84  DVRAFGAIGNGVADDTEAFKMAWDMACQSDDSAVMLVPYSFSFMIQSTIFTGPCHGHLML 143
           DV +FGA+G+G  DDT AF+ AW  AC + +S V+L P    F I STIF+GPC   L+ 
Sbjct: 85  DVTSFGAVGDGSCDDTAAFQDAWKAAC-AVESGVVLAPEGGVFKITSTIFSGPCKPGLVF 144

Query: 144 RVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWWDLPCKPHKGIN 203
           ++DG LMPPDGP+ WP+  ++ QWLVFYR+DG +  G G ++G GQKWWDLPCKPH+G +
Sbjct: 145 QLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGKGTVEGNGQKWWDLPCKPHRGPD 204

Query: 204 GTTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFHFRFDGCKNVHIDSIHIKSPGLSPN 263
           G++  GPC SP  IRFFMS+N+ V+GL+I NSPQFH +FDGC+ V I+ I I SP LSPN
Sbjct: 205 GSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMKFDGCQGVLINEIQISSPKLSPN 264

Query: 264 TDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVDIRNITCGPSHGISIGSLGVRNSRA 323
           TDGIH+ NT  V IYNSV+ NGDDC+SIG+G  DVDI+ +TCGPSHGISIGSLGV NS+A
Sbjct: 265 TDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDIQGVTCGPSHGISIGSLGVHNSQA 324

Query: 324 CVTNITVRDSVIKVSDNGVRIKTWQGGYGAVKGLTFSNIHMDNVRNPIIIDQYYCLTKDC 383
           CV+NITVR++VI+ SDNG+R+KTWQGG G+V  L F NI M+NV N II+DQYYC +KDC
Sbjct: 325 CVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFENIQMENVLNCIIVDQYYCQSKDC 384

Query: 384 TNQTSAVSISDIQYTNIKGTYDIRSPPMHFACSDSVPCTNLTLSEIELLPAQGDIVLDPF 443
            N+TSAV + D++Y NIKGTYD+RSPP+HFACSD+V CTN+T+SE+ELLP +G++V DPF
Sbjct: 385 RNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVACTNITMSEVELLPEEGELVDDPF 444

Query: 444 CWNAYGDLQTLTIPPVSCLQEGLP 462
           CWNAYG  +TLTIPP+ CL +G P
Sbjct: 445 CWNAYGKQETLTIPPIDCLLDGSP 463

BLAST of Tan0008749 vs. TAIR 10
Match: AT1G60590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 380.9 bits (977), Expect = 1.4e-105
Identity = 194/481 (40.33%), Postives = 285/481 (59.25%), Query Frame = 0

Query: 29  HHHTKQKHSHHHK-----------HSYISHPPSISHPPSPSPSPSDSPDDGDYIGETNVF 88
           HHH   K     K              +S PP +  PP P   P  +P     + ++  F
Sbjct: 64  HHHMSHKSKPKPKMKTQPPKSNDGSPVVSQPPQVQQPPPPHVQPPPTPLPLQPVEDSQQF 123

Query: 89  DVRAFGAIGNGVADDTEAFKMAWDMACQSDDSAVMLVPYSFSFMIQSTIFTGP-CHGHLM 148
           +V  FGA G+G++DDT+AF+ AW  AC+  +++ M++P  + F++    F+GP C  +++
Sbjct: 124 NVLDFGAKGDGMSDDTQAFEAAWASACKV-EASTMIIPPDYIFLVGPISFSGPYCQANIV 183

Query: 149 LRVDGTLMPPDGPDAWPKNASRRQWLVFYRIDGMSLEGGGVIDGRGQKWW------DLPC 208
            +++G ++ P   ++W        W+ F ++ G++++G GVIDGRG  WW      D P 
Sbjct: 184 FQLEGMIVAPTDTESW--GGGLMWWIEFTKLSGITIQGNGVIDGRGTVWWQQDYLSDYPI 243

Query: 209 ----------------KPHKGING-TTLPGPCDSPIAIRFFMSSNLTVQGLKITNSPQFH 268
                           +P   I        P   P A+RF+ S ++TV G+ I NSPQ H
Sbjct: 244 DDDFKLIVPLNNSVQERPPMPIRSELNWRMPSIKPTALRFYGSIDVTVTGITIQNSPQCH 303

Query: 269 FRFDGCKNVHIDSIHIKSPGLSPNTDGIHIENTNGVEIYNSVIYNGDDCVSIGSGSYDVD 328
            +FD C  V +  +++ SPG SPNTDGIH++NT  V I+ + +  GDDC+SI +G  +V 
Sbjct: 304 LKFDNCVKVLVHDVNVSSPGDSPNTDGIHLQNTRDVMIHTTTLACGDDCISIQTGCSNVY 363

Query: 329 IRNITCGPSHGISIGSLGVRNSRACVTNITVRDSVIKVSDNGVRIKTWQGGYGAVKGLTF 388
           + N+ CGP HGISIGSLG  +++ACV+NITVRD V+  +  GVRIKTWQGG G+VKG+ F
Sbjct: 364 VHNVNCGPGHGISIGSLGKDSTKACVSNITVRDVVMHNTMTGVRIKTWQGGIGSVKGILF 423

Query: 389 SNIHMDNVRNPIIIDQYYCLTKDCTNQTSAVSISDIQYTNIKGTYDIRSPPMHFACSDSV 448
           SNI +  V+ PI+IDQ+YC    C N TSAVS+  + Y  I+GTY ++  P+HFACSDS 
Sbjct: 424 SNIQLTEVQLPIVIDQFYCDHSKCMNHTSAVSVEGVTYEKIRGTYTVK--PVHFACSDSF 483

Query: 449 PCTNLTLSEIELLPAQGDI-VLDPFCWNAYGDLQTLTIPPVSCLQEGLPSSINLNNDHEY 474
           PC ++ LS IEL P Q    + DPFCW  +G+L + T+PP+ CLQ G P+   +++DH+ 
Sbjct: 484 PCIDVQLSGIELKPVQLQYHMYDPFCWKTFGELNSATVPPIDCLQIGKPARNGVHSDHDI 539

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q949Z17.1e-15859.23Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV... [more]
Q94AJ51.4e-9244.33Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017... [more]
P432128.9e-8444.01Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1[more]
Q7M1E78.3e-8244.38Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
P353364.1e-8141.67Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038899064.17.3e-26994.30polygalacturonase At1g48100-like [Benincasa hispida][more]
TYJ96898.14.3e-26191.79polygalacturonase [Cucumis melo var. makuwa][more]
XP_008461394.11.3e-26091.58PREDICTED: polygalacturonase At1g48100-like [Cucumis melo][more]
XP_022146163.13.1e-25990.49polygalacturonase At1g48100 [Momordica charantia][more]
XP_004135975.11.3e-25790.74polygalacturonase At1g48100 [Cucumis sativus] >KGN45046.1 hypothetical protein C... [more]
Match NameE-valueIdentityDescription
A0A5D3BFC72.1e-26191.79Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold670G00... [more]
A0A1S3CFX86.1e-26191.58polygalacturonase At1g48100-like OS=Cucumis melo OX=3656 GN=LOC103499990 PE=3 SV... [more]
A0A6J1CXU81.5e-25990.49polygalacturonase At1g48100 OS=Momordica charantia OX=3673 GN=LOC111015443 PE=3 ... [more]
A0A0A0KBA76.3e-25890.74Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G414430 PE=3 SV=1[more]
A0A6J1GP581.2e-25689.22polygalacturonase At1g48100 OS=Cucurbita moschata OX=3662 GN=LOC111456199 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT1G02460.18.8e-18867.47Pectin lyase-like superfamily protein [more]
AT4G01890.12.7e-16860.68Pectin lyase-like superfamily protein [more]
AT1G56710.12.0e-16063.24Pectin lyase-like superfamily protein [more]
AT1G48100.15.0e-15959.23Pectin lyase-like superfamily protein [more]
AT1G60590.11.4e-10540.33Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 348..369
e-value: 1000.0
score: 7.1
coord: 239..265
e-value: 94.0
score: 11.9
coord: 289..309
e-value: 6600.0
score: 1.4
coord: 319..340
e-value: 14.0
score: 14.6
coord: 266..287
e-value: 90.0
score: 11.9
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 104..425
e-value: 1.7E-88
score: 296.8
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 296..309
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 71..463
e-value: 4.6E-119
score: 400.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 39..69
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 34..462
NoneNo IPR availablePANTHERPTHR31375:SF119POLYGALACTURONASE/GLYCOSIDE HYDROLASE FAMILY PROTEINcoord: 34..462
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 74..470

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0008749.1Tan0008749.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0016829 lyase activity
molecular_function GO:0004650 polygalacturonase activity