Tan0008492 (gene) Snake gourd v1

Overview
NameTan0008492
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionNAD(P)-binding Rossmann-fold superfamily protein
LocationLG04: 330281 .. 334329 (+)
RNA-Seq ExpressionTan0008492
SyntenyTan0008492
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GATAATTCTTTTCACCGTACAAGATTTGTCCCTCTCGAAAGGGTTTAGAAATGGCGGGGTGGGCCCGCCTTTCTCTCAGCTCCCACGTTCCTAATTTGATCTATATCCTTCAGTTTCCCACTCCGCCTCTTCCCGTTTCCCATCTCTATTTAGTATTTATATTCCCCTCTTCACTTCTCGATTTCCATGCCTGAGGCTGATTATTCCAGATTCACATTCACTCATCTCTTTCTTCCATGGATTCCAAGGAATTCGACCTCTCTTCTCCTTCCTCTTCTCCCTTCTCCGACAGGTAATCGATTAAACCTCCGTCGCTGATCTGATCTTTTCATCTCCTTTTTCTGATCCGTTTTTAATTATACTCTTTCTTTGGCCGTGCAGATGGTGGTCGAATAACTCAGTGGCGGTCGTTACCGGAGCCAATAAGGGGATCGGATTTGCCCTAGTTAGAAGATTAGCGCAATTGGAACTCACTGTGATATTAACCGCTAGGGACGAAGAAAGAGGACTGAAGGCCGTTGAAATGCTACAAAATGAAGGACTGAACGTTCTGTTTCGCCGCCTCGATGTTTCCGATCCTGTCTCCATCAAAGCCTTCGCGGCCTGGTTCATCGCCAATTTTCAAGCCCTCGATATTCTTGTAAGTATTTATGACTTATGACTCATGAGTTCATATCTATGGATTCAATCTGTCCTCTTTTGCCCCTTTAATTTGTTTTTTCTTCTTTTCAATCGAATTTCGCGCATTAACTTCTCTCTTTCTGCTGTGCTTGTGCACCATTCATTTCATTCACGCTCAGCTCACACCTCCTGTTTTTTCCAGAACTACTTTTCATTTTTTCTGAATTTACAATTTCAACCCTCAACTTGCATGAAATTATAATTCCTCGCAACTCTGGCATTATTCTCTTACGTCACGTCACGGGTTAATAAACAGTTGGTTTTAGTCGATGATGCGACTGTGCAAGCTATTCTGTTGTGTTATTGTGTCACTTGCATAATTATTGAGACTCGTCTTTTTATAAAAAAAAAATCATAATTTAGGCCATTCTTAGTTCTACTTCTTATGACCATTTTATTTTTTTGTCATCCATTTCTTTCTTTTTTACCATTTATTTGTTGCATTCCTTACTTTAATCAATTTTTAGAAACTTAAAAGAAATTAAAAAACTTATTGTCGTGAAAAAGTTATTTTTGAGTTTGAATTTATACTCTATTTTGTTCTATTCATTCCCCTTCGTTCAATATTGATTTTATATAACTTCACATTATGGGGGTGTGCTACATTAAATTTTGCGGTGTGTATTTAACATAAATGGTACATGATGCCCTAATATGTTATTTAAGCTACACAGAAATGTGTGATATTTAGCATGAAGGGTAAAATTCAGAATGGTTCTTTAATGAAACAAATGTGTAATTTAAACCTTTTTTTTTCAAGCCAATTTACCCAGCGGGTTGAGGTAAGTACATCTTTTCATTGAGTTAGAAATTAGATCAAATCTTCATCCTTGTAATGATATTTTTTTTTAAGTTTAATTTATAAGTTTAGTGAATGAATTTTCAAGATTATGTTTAATACATCTTTAAATTTTTTATTTTACGTTGAATAACAATCTTACCATTTTTAAAACTTATTGATATGTTTCAATTTTCTGGGTAATAGAGTTTGTGAATTTTAAAAAATGTCTAATTGTTTAGGAATTTATTATACATGAAATTGAAAGTTGATGAGTTTATTACATATTTAAATGTCATATTAGATAAAAAATTGAAAGTTAGACCTTGTTTGATAACCATTTGATTTTTAAAATGAATTTTTTTTTCTCTCAATTTCTCTACAATGATTTTCATCTCTGTTACAGAGAAACATTTGAATTCGTAGTGAAATTCCAAAAACATTTTTTTTTTTTAGCTTTTAGAGTTTGCCTTGTTTTTTAAAACTTATGTGAAAAGCAGTTGAAATTCATAATTAGTCTATTTAGACTTAATTTTTTTTAAAAAAAAAAAAAGCTAAAAAATCAAATAGTTATCCTTAGTGTCCTATTTAATTCCAGGTTTCAAGATTTATTAACAGAACGGAACTAAACTATGAGGAAATGATCACTATTATGCAACAATCATATATATATATATATATATATATATATATATACCTATGCAGCCCAATGGAGGCATAGCCACAATTATTTTATTTTATTTTATTTTTTTAAAAAAAAAAACTTTTATGGTTAAAATATGTGCAGCTAAGTGCAGCCCATATATATAAATATGAGCAAACTTTAACTGTAGTTAGCCGAGGAATCTCTTCTGTTTATGGTAATGGTAGGTAGGGCTACGCTTAATTTATGAAAGTTATAATCAAGAACTGCAAACAAACAAAAAAAAAGTGTTGCAGAGAAGAGAACACTTGGTTTAAAGTCAACAAAGATGACATTGTGAAAGAAATAAATAGCTTTCTTCTTTTTCTCTGTGAGGAAAGAAATAAGCAAGGTGATTGGATGTTCCCCATCAATTTGGTCGTTTTAGATGTTCCTATTTTGTCTCGGAGTCCCTTTCTTTTTATGTTATGTGTATTTTGAAAAGATTACCATTTTTTTTTTTTGTTTACCCTATATTCTTGAGTTTATAAATAAATTTTAAATATCGACAGATGTTGCATCAAATCAGTTTTAAATATTGACAGATCCCGCCTAATTTAAAAAAGAAAATGCTGACATGAAGGGAGATAAAATAAAAAAAAAATCCATACTAATGCTTTTCCGGTATTTCCACTTGAACTGCCATTGTCCATCCTCCATAACGACAAGGCAATAAGTCAAGCACTTTGACGCCCACTCCATCTAGGAAAAGTCTCAATATCAAGACCACGAGGGCAATATTTTAAACACATGGTACAATAATAATATTCCAATCAATTATAAAAACAAAACCTACAGACTTTTCTTTTTTTTCTTTTTGTTCCATCAATTGGACCATATTGTAAAATAATGTAATTTAAATAGCTATAATATACTGTAGCCTGTAGGTAGGACCAGGACCAGCATAACATTATTACATAACTGAGATATATCATTAGGTTTTTGATACAATATTATTATTATATTCACTTTCATTAAAAAAAAAATGATTTACTTTTGCAGGTTAACAACGCAGCCGTATCATTCAACGACATAAACGAGAACTCAGTGGAAAACGCAGAAACAGTAATAAAAACCAACTTTTACGGCGCAAAATTACTGACCGAGGCTCTGTTGCCTTTCTTCCGCTCTTCTGCTTCCAGAAGCCGAATTCTCAACATCACTTCTAGGCTGGGAACCATCGATGTAACCCTTAATTAAACAAATTCAATTAATTAATAATAAATGAAGATTGATTATATTAATAATGAAAAAATTGCAGAAGGTGAAGAACAAGAAAGTAAAAGAGATTCTGCAGAGTGAAGATCTAGCGGAGGAGGAGATCGACGGCGTAGTGAATGCATTTCTGGAGGACGTGAAGAGAGGGAGATGGAAGAAGCAAGGCTGGCCGGCGCTGTGGACGGACTATGCAGTATCGAAGCTGGCGCTAAACTCTTACGCGAGAGTCTTGGCGAAACGATACGGTGCGTTTGGGAGTGTGAGTGTGAACTGCTTTTGCCCAGGCTTCACACAGACTTCAATGACACGTGGGAAAGGGACACACACCGCCGACGCCGCCGCACTTGTCGGAGCTAGGCTGGCCTTGCTGCCGCCTCAGCGTTTACCAACTGGTCAGTTCTTCTCGTGGGGGCCTAGTTATGACACACCCAGAAACTCCAAATTATAGCTAAATTAAAATTATTGGTCTTCTAATTTATAATTTTTATATATAGACATTCCTTACTTTAATTTGGAGTTATTTTCAGCGGCTGCTAGTCTTGGGTCTCTCTCTGTCTCTGTACAATGCAATTGCAATCAATCTCTCTGCTTTAATGTGGTCTTCTCTATTATTTTGTTATCACTTTACACCAATATTGTTAATTAGTTTACATGTTGGTCGTCTAGCTAGTATTTTGTTTGCCCCCAACG

mRNA sequence

GATAATTCTTTTCACCGTACAAGATTTGTCCCTCTCGAAAGGGTTTAGAAATGGCGGGGTGGGCCCGCCTTTCTCTCAGCTCCCACGTTCCTAATTTGATCTATATCCTTCAGTTTCCCACTCCGCCTCTTCCCGTTTCCCATCTCTATTTAGTATTTATATTCCCCTCTTCACTTCTCGATTTCCATGCCTGAGGCTGATTATTCCAGATTCACATTCACTCATCTCTTTCTTCCATGGATTCCAAGGAATTCGACCTCTCTTCTCCTTCCTCTTCTCCCTTCTCCGACAGATGGTGGTCGAATAACTCAGTGGCGGTCGTTACCGGAGCCAATAAGGGGATCGGATTTGCCCTAGTTAGAAGATTAGCGCAATTGGAACTCACTGTGATATTAACCGCTAGGGACGAAGAAAGAGGACTGAAGGCCGTTGAAATGCTACAAAATGAAGGACTGAACGTTCTGTTTCGCCGCCTCGATGTTTCCGATCCTGTCTCCATCAAAGCCTTCGCGGCCTGGTTCATCGCCAATTTTCAAGCCCTCGATATTCTTGTTAACAACGCAGCCGTATCATTCAACGACATAAACGAGAACTCAGTGGAAAACGCAGAAACAGTAATAAAAACCAACTTTTACGGCGCAAAATTACTGACCGAGGCTCTGTTGCCTTTCTTCCGCTCTTCTGCTTCCAGAAGCCGAATTCTCAACATCACTTCTAGGCTGGGAACCATCGATAAGGTGAAGAACAAGAAAGTAAAAGAGATTCTGCAGAGTGAAGATCTAGCGGAGGAGGAGATCGACGGCGTAGTGAATGCATTTCTGGAGGACGTGAAGAGAGGGAGATGGAAGAAGCAAGGCTGGCCGGCGCTGTGGACGGACTATGCAGTATCGAAGCTGGCGCTAAACTCTTACGCGAGAGTCTTGGCGAAACGATACGGTGCGTTTGGGAGTGTGAGTGTGAACTGCTTTTGCCCAGGCTTCACACAGACTTCAATGACACGTGGGAAAGGGACACACACCGCCGACGCCGCCGCACTTGTCGGAGCTAGGCTGGCCTTGCTGCCGCCTCAGCGTTTACCAACTGGTCAGTTCTTCTCGTGGGGGCCTAGTTATGACACACCCAGAAACTCCAAATTATAGCTAAATTAAAATTATTGGTCTTCTAATTTATAATTTTTATATATAGACATTCCTTACTTTAATTTGGAGTTATTTTCAGCGGCTGCTAGTCTTGGGTCTCTCTCTGTCTCTGTACAATGCAATTGCAATCAATCTCTCTGCTTTAATGTGGTCTTCTCTATTATTTTGTTATCACTTTACACCAATATTGTTAATTAGTTTACATGTTGGTCGTCTAGCTAGTATTTTGTTTGCCCCCAACG

Coding sequence (CDS)

ATGGATTCCAAGGAATTCGACCTCTCTTCTCCTTCCTCTTCTCCCTTCTCCGACAGATGGTGGTCGAATAACTCAGTGGCGGTCGTTACCGGAGCCAATAAGGGGATCGGATTTGCCCTAGTTAGAAGATTAGCGCAATTGGAACTCACTGTGATATTAACCGCTAGGGACGAAGAAAGAGGACTGAAGGCCGTTGAAATGCTACAAAATGAAGGACTGAACGTTCTGTTTCGCCGCCTCGATGTTTCCGATCCTGTCTCCATCAAAGCCTTCGCGGCCTGGTTCATCGCCAATTTTCAAGCCCTCGATATTCTTGTTAACAACGCAGCCGTATCATTCAACGACATAAACGAGAACTCAGTGGAAAACGCAGAAACAGTAATAAAAACCAACTTTTACGGCGCAAAATTACTGACCGAGGCTCTGTTGCCTTTCTTCCGCTCTTCTGCTTCCAGAAGCCGAATTCTCAACATCACTTCTAGGCTGGGAACCATCGATAAGGTGAAGAACAAGAAAGTAAAAGAGATTCTGCAGAGTGAAGATCTAGCGGAGGAGGAGATCGACGGCGTAGTGAATGCATTTCTGGAGGACGTGAAGAGAGGGAGATGGAAGAAGCAAGGCTGGCCGGCGCTGTGGACGGACTATGCAGTATCGAAGCTGGCGCTAAACTCTTACGCGAGAGTCTTGGCGAAACGATACGGTGCGTTTGGGAGTGTGAGTGTGAACTGCTTTTGCCCAGGCTTCACACAGACTTCAATGACACGTGGGAAAGGGACACACACCGCCGACGCCGCCGCACTTGTCGGAGCTAGGCTGGCCTTGCTGCCGCCTCAGCGTTTACCAACTGGTCAGTTCTTCTCGTGGGGGCCTAGTTATGACACACCCAGAAACTCCAAATTATAG

Protein sequence

MDSKEFDLSSPSSSPFSDRWWSNNSVAVVTGANKGIGFALVRRLAQLELTVILTARDEERGLKAVEMLQNEGLNVLFRRLDVSDPVSIKAFAAWFIANFQALDILVNNAAVSFNDINENSVENAETVIKTNFYGAKLLTEALLPFFRSSASRSRILNITSRLGTIDKVKNKKVKEILQSEDLAEEEIDGVVNAFLEDVKRGRWKKQGWPALWTDYAVSKLALNSYARVLAKRYGAFGSVSVNCFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFSWGPSYDTPRNSKL
Homology
BLAST of Tan0008492 vs. ExPASy Swiss-Prot
Match: Q9M2E2 ((+)-neomenthol dehydrogenase OS=Arabidopsis thaliana OX=3702 GN=SDR1 PE=1 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 6.5e-48
Identity = 120/285 (42.11%), Postives = 162/285 (56.84%), Query Frame = 0

Query: 27  AVVTGANKGIGFALVRRLAQLELTVILTARDEERGLKAVEMLQNE----GLNVLFRRLDV 86
           AVVTGAN+GIGF + R+LA   + V+LT+RDE RGL+AVE L+ E      ++LF +LDV
Sbjct: 9   AVVTGANRGIGFEICRQLASEGIRVVLTSRDENRGLEAVETLKKELEISDQSLLFHQLDV 68

Query: 87  SDPVSIKAFAAWFIANFQALDILVNNAAV-------------------SFNDINENSVEN 146
           +DP SI + A +    F  LDILVNNA +                    +++I   + E 
Sbjct: 69  ADPASITSLAEFVKTQFGKLDILVNNAGIGGIITDAEALRAGAGKEGFKWDEIITETYEL 128

Query: 147 AETVIKTNFYGAKLLTEALLPFFRSSASRSRILNITSRLGTIDKVKNKKVKEIL-QSEDL 206
            E  IK N+YG K + EA +P  + S S  RI+N++S +G +  V N+  K IL  +E+L
Sbjct: 129 TEECIKINYYGPKRMCEAFIPLLKLSDS-PRIVNVSSSMGQLKNVLNEWAKGILSDAENL 188

Query: 207 AEEEIDGVVNAFLEDVKRGRWKKQGWPALWTDYAVSKLALNSYARVLAKRYGAFGSVSVN 266
            EE ID V+N  L D K G  K++ W    + Y VSK +LN Y RVLAK++  F    VN
Sbjct: 189 TEERIDQVINQLLNDFKEGTVKEKNWAKFMSAYVVSKASLNGYTRVLAKKHPEF---RVN 248

Query: 267 CFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFS 288
             CPGF +T M    G  + +  A    RLALLP Q  P+G FFS
Sbjct: 249 AVCPGFVKTDMNFKTGVLSVEEGASSPVRLALLPHQETPSGCFFS 289

BLAST of Tan0008492 vs. ExPASy Swiss-Prot
Match: A4UHT7 (Salutaridine reductase OS=Papaver bracteatum OX=215227 GN=SALR PE=1 SV=1)

HSP 1 Score: 183.7 bits (465), Expect = 3.0e-45
Identity = 124/294 (42.18%), Postives = 161/294 (54.76%), Query Frame = 0

Query: 27  AVVTGANKGIGFALVRRLAQLELTVILTARDEERGLKAVEMLQNEG-LNVLFRRLDVSDP 86
           AVVTG NKGIGF + ++L+   + V+LT RD  RGL+AVE L+N    NV+F +LDV+DP
Sbjct: 15  AVVTGGNKGIGFEICKQLSSSGIMVVLTCRDVTRGLEAVEKLKNSNHENVVFHQLDVTDP 74

Query: 87  VSIKAFAAWFI-ANFQALDILVNNAAVS------------FNDINENS------------ 146
           ++  +  A FI A F  LDILVNNA V+             +DI E+S            
Sbjct: 75  ITTMSSLADFIKARFGKLDILVNNAGVAGFSVDADRFKAMISDIGEDSEEVVKIYEKPEA 134

Query: 147 -------VENAETVIKTNFYGAKLLTEALLPFFRSSASRSRILNITSRLGTIDKVKNKKV 206
                   E AE  +K N+YG K +TE LLP  + S S  RI+N++S  G++  V N+  
Sbjct: 135 QELMSETYELAEECLKINYYGVKSVTEVLLPLLQLSDS-PRIVNVSSSTGSLKYVSNETA 194

Query: 207 KEILQSED-LAEEEIDGVVNAFLEDVKRGRWKKQGWPALWTDYAVSKLALNSYARVLAKR 266
            EIL   D L EE ID VVN  L+D K    +  GWP+    Y  SK  LN+Y RVLAK+
Sbjct: 195 LEILGDGDALTEERIDMVVNMLLKDFKENLIETNGWPSFGAAYTTSKACLNAYTRVLAKK 254

Query: 267 YGAFGSVSVNCFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFF 287
              F    VNC CPG  +T M  G G +TAD  A    R+AL P    P+G F+
Sbjct: 255 IPKF---QVNCVCPGLVKTEMNYGIGNYTADEGAKHVVRIALFPDDG-PSGFFY 303

BLAST of Tan0008492 vs. ExPASy Swiss-Prot
Match: Q9ZUH5 (Short-chain dehydrogenase/reductase 2b OS=Arabidopsis thaliana OX=3702 GN=SDR2b PE=1 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 3.3e-44
Identity = 112/284 (39.44%), Postives = 161/284 (56.69%), Query Frame = 0

Query: 27  AVVTGANKGIGFALVRRLAQLELTVILTARDEERGLKAVEMLQNE----GLNVLFRRLDV 86
           A+VTG N+GIGF + R+LA   + VILT+RDE++GL+AVE L+ E      +++F +LDV
Sbjct: 9   AIVTGGNRGIGFEICRQLANKGIRVILTSRDEKQGLEAVETLKKELEISDQSIVFHQLDV 68

Query: 87  SDPVSIKAFAAWFIANFQALDILVNNAAV-------------------SFNDINENSVEN 146
           SDPVS+ + A +   +F  LDIL+NNA V                    + +    + E 
Sbjct: 69  SDPVSVTSLAEFVKTHFGKLDILINNAGVGGVITDVDALRAGTGKEGFKWEETITETYEL 128

Query: 147 AETVIKTNFYGAKLLTEALLPFFRSSASRSRILNITSRLGTIDKVKNKKVKEIL-QSEDL 206
           AE  IK N+YG K + EA +P  + S S  RI+N++S +G +  + N+  K IL  +E+L
Sbjct: 129 AEECIKINYYGPKRMCEAFIPLLQLSDS-PRIINVSSFMGQVKNLVNEWAKGILSDAENL 188

Query: 207 AEEEIDGVVNAFLEDVKRGRWKKQGWPALWTDYAVSKLALNSYARVLAKRYGAFGSVSVN 266
            E  ID V+N  L D+K    K + W  + + Y VSK  LN+Y R+LAK++     + VN
Sbjct: 189 TEVRIDQVINQLLNDLKEDTAKTKYWAKVMSAYVVSKAGLNAYTRILAKKH---PEIRVN 248

Query: 267 CFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFF 287
             CPGF +T M    G  + +  A    RLALLP Q  P+G FF
Sbjct: 249 SVCPGFVKTDMNFKTGILSVEEGASSPVRLALLPHQESPSGCFF 288

BLAST of Tan0008492 vs. ExPASy Swiss-Prot
Match: Q6WAU1 ((-)-isopiperitenone reductase OS=Mentha piperita OX=34256 PE=1 SV=1)

HSP 1 Score: 171.0 bits (432), Expect = 2.0e-41
Identity = 118/304 (38.82%), Postives = 168/304 (55.26%), Query Frame = 0

Query: 27  AVVTGANKGIGFALVRRLAQLELTVILTARDEERGLKAVEMLQNEGLNV-----LFRRLD 86
           A+VTGANKGIGF + R+LA+  + VILT+R+E+RGL+A + L  E LNV     +F +LD
Sbjct: 8   ALVTGANKGIGFEICRQLAEKGIIVILTSRNEKRGLEARQKLLKE-LNVSENRLVFHQLD 67

Query: 87  VSDPVSIKAFAAWFIANFQALDILVNNAAVS----------FNDINE------------- 146
           V+D  S+ A A +  + F  LDILVNNA VS          FN+  E             
Sbjct: 68  VTDLASVAAVAVFIKSKFGKLDILVNNAGVSGVEMVGDVSVFNEYIEADFKALQALEAGA 127

Query: 147 ---------------NSVENAETVIKTNFYGAKLLTEALLPFFRSSASRSRILNITSRLG 206
                             E A+  + TN+YG K LT+AL+P  + S S  RI+N++S  G
Sbjct: 128 KEEPPFKPKANGEMIEKFEGAKDCVVTNYYGPKRLTQALIPLLQLSPS-PRIVNVSSSFG 187

Query: 207 TIDKVKNKKVKEILQSED-LAEEEIDGVVNAFLEDVKRGRWKKQGWPALWTDYAVSKLAL 266
           ++  + N+  K +L  ED L EE +D VV  FL+D+K G+ ++  WP  +    VSK AL
Sbjct: 188 SLLLLWNEWAKGVLGDEDRLTEERVDEVVEVFLKDIKEGKLEESQWPPHFAAERVSKAAL 247

Query: 267 NSYARVLAKRYGAFGSVSVNCFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPT 287
           N+Y ++ AK+Y +F    +N  CPG+ +T +T   G  +   AA V  +LALLP    P+
Sbjct: 248 NAYTKIAAKKYPSF---RINAICPGYAKTDITFHAGPLSVAEAAQVPVKLALLPDGG-PS 305

BLAST of Tan0008492 vs. ExPASy Swiss-Prot
Match: B2X050 ((+)-neomenthol dehydrogenase OS=Capsicum annuum OX=4072 GN=MNR1 PE=1 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.7e-40
Identity = 113/301 (37.54%), Postives = 161/301 (53.49%), Query Frame = 0

Query: 27  AVVTGANKGIGFALVRRLAQLELTVILTARDEERGLKAVEMLQNEG----LNVLFRRLDV 86
           AVVTG NKGIG+   R+LA   + V+LT+RDE++G++A+E L+ E      ++LF +LD+
Sbjct: 11  AVVTGGNKGIGYETCRQLASKGVVVVLTSRDEKKGIEAIERLKEESNFTDEHILFHQLDI 70

Query: 87  SDPVSIKAFAAWFIANFQALDILVNNAAVS----------FNDINENSV-------ENAE 146
            DP SI +        F  LDIL+NNA +S            +I E  +       EN E
Sbjct: 71  MDPASISSLVNLIKTKFGRLDILINNAGISGVMVEGDVQVLKEILERYISIVFTEDENGE 130

Query: 147 -------------------TVIKTNFYGAKLLTEALLPFFRSSASRSRILNITSRLGTID 206
                                I+TN+YGAK +TEA +P  + S S  RI+N+ S +G + 
Sbjct: 131 EGGWTKSGPGSVTNYELTKECIETNYYGAKRMTEAFIPLLQLSNS-PRIVNVASSMGKLK 190

Query: 207 KVKNKKVKEILQSED-LAEEEIDGVVNAFLEDVKRGRWKKQGWPALWTDYAVSKLALNSY 266
            + NK   E+L+  D L EE++D VVN FL+D      + +GWP+ +T Y VSK +L +Y
Sbjct: 191 LLCNKWAIEVLRDADSLTEEKVDQVVNEFLKDFTEKSTESKGWPSYFTAYKVSKASLIAY 250

Query: 267 ARVLAKRYGAFGSVSVNCFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQF 287
            RVLA +Y  F    +N  CPG+ +T +    G+ TA   A     LALLP    P+G F
Sbjct: 251 TRVLATKYPNF---RINSVCPGYCKTDVNANTGSLTAGEGAESLVNLALLPNDG-PSGLF 306

BLAST of Tan0008492 vs. NCBI nr
Match: KAG6573598.1 ((+)-neomenthol dehydrogenase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7012680.1 (+)-neomenthol dehydrogenase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 513.8 bits (1322), Expect = 9.7e-142
Identity = 263/300 (87.67%), Postives = 280/300 (93.33%), Query Frame = 0

Query: 1   MDSKEFDLSSPSSSPFSDRWWSNNSVAVVTGANKGIGFALVRRLAQLELTVILTARDEER 60
           MDSKEFDLSS SSSPFS+RWWSNN+VAVVTGANKGIGFALVRRLAQ+ELTVILT R+E R
Sbjct: 1   MDSKEFDLSSASSSPFSNRWWSNNTVAVVTGANKGIGFALVRRLAQMELTVILTTRNEGR 60

Query: 61  GLKAVEMLQNEGLNVLFRRLDVSDPVSIKAFAAWFIANFQALDILVNNAAVSFNDINENS 120
           GLKAVE L+NEGLNVLFRRLDVSDP SI AFAAWF+ANF ALDILVNNAAVSFNDI+ NS
Sbjct: 61  GLKAVESLRNEGLNVLFRRLDVSDPASINAFAAWFVANFPALDILVNNAAVSFNDIDHNS 120

Query: 121 VENAETVIKTNFYGAKLLTEALLPFFRSSASRSRILNITSRLGTIDKVKNKKVKEILQSE 180
           VENAETVIKTNFYGAKLLT+ALLP FRSS+SRSRILNITSRLGTIDKVKN+KVKEILQSE
Sbjct: 121 VENAETVIKTNFYGAKLLTQALLPLFRSSSSRSRILNITSRLGTIDKVKNEKVKEILQSE 180

Query: 181 DLAEEEIDGVVNAFLEDVKRGRWKKQGWPALWTDYAVSKLALNSYARVLAKRYGAFGSVS 240
           DLAEE+IDGVV+AFL+DVKRG WKK+GWPALWTDYAVSKLALN+Y RVLAKR     SVS
Sbjct: 181 DLAEEDIDGVVDAFLQDVKRGTWKKKGWPALWTDYAVSKLALNAYTRVLAKR---SRSVS 240

Query: 241 VNCFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFSWGPSYDTPRNSKL 300
           VN FCPGFTQTSMT GKGTHTADAAA+VGARLALLPP RLPTGQFF WGP+YDTPRNSKL
Sbjct: 241 VNSFCPGFTQTSMTGGKGTHTADAAAVVGARLALLPPHRLPTGQFFYWGPNYDTPRNSKL 297

BLAST of Tan0008492 vs. NCBI nr
Match: XP_022945555.1 ((+)-neomenthol dehydrogenase-like [Cucurbita moschata])

HSP 1 Score: 513.8 bits (1322), Expect = 9.7e-142
Identity = 263/300 (87.67%), Postives = 280/300 (93.33%), Query Frame = 0

Query: 1   MDSKEFDLSSPSSSPFSDRWWSNNSVAVVTGANKGIGFALVRRLAQLELTVILTARDEER 60
           MDSKEFDLSS SSSPFSDRWWSNN+VAVVTGANKGIGFALVRRLAQ+ELTVILT R+E R
Sbjct: 1   MDSKEFDLSSASSSPFSDRWWSNNTVAVVTGANKGIGFALVRRLAQMELTVILTTRNEGR 60

Query: 61  GLKAVEMLQNEGLNVLFRRLDVSDPVSIKAFAAWFIANFQALDILVNNAAVSFNDINENS 120
           GLKAVE L+NEGLNVLFRRLDVSDP SI AFAAW +ANF ALDILVNNAAVSFNDI+ NS
Sbjct: 61  GLKAVESLRNEGLNVLFRRLDVSDPASINAFAAWVVANFPALDILVNNAAVSFNDIDHNS 120

Query: 121 VENAETVIKTNFYGAKLLTEALLPFFRSSASRSRILNITSRLGTIDKVKNKKVKEILQSE 180
           VENAETVIKTNFYGAKLLT+ALLP FRSS+SRSRILNITSRLGTIDKVKN+KVKEILQSE
Sbjct: 121 VENAETVIKTNFYGAKLLTQALLPLFRSSSSRSRILNITSRLGTIDKVKNEKVKEILQSE 180

Query: 181 DLAEEEIDGVVNAFLEDVKRGRWKKQGWPALWTDYAVSKLALNSYARVLAKRYGAFGSVS 240
           DLAEE+I+GVV+AFL+DVKRG WKK+GWPALWTDYAVSKLALN+Y RVLAKR     SVS
Sbjct: 181 DLAEEDIEGVVDAFLQDVKRGTWKKKGWPALWTDYAVSKLALNAYTRVLAKR---SRSVS 240

Query: 241 VNCFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFSWGPSYDTPRNSKL 300
           VN FCPGFTQTSMT GKGTHTADAAA+VGARLALLPP RLPTGQFFSWGP+YDTPRNSKL
Sbjct: 241 VNSFCPGFTQTSMTGGKGTHTADAAAVVGARLALLPPHRLPTGQFFSWGPNYDTPRNSKL 297

BLAST of Tan0008492 vs. NCBI nr
Match: XP_022957511.1 ((+)-neomenthol dehydrogenase-like [Cucurbita moschata])

HSP 1 Score: 512.7 bits (1319), Expect = 2.2e-141
Identity = 260/300 (86.67%), Postives = 278/300 (92.67%), Query Frame = 0

Query: 1   MDSKEFDLSSPSSSPFSDRWWSNNSVAVVTGANKGIGFALVRRLAQLELTVILTARDEER 60
           M+SKEFDLSS  SSP SDRWWS N+VAVVTGANKGIGFALVRRLAQ ELTVILTARDE R
Sbjct: 1   MESKEFDLSSSLSSPVSDRWWSKNTVAVVTGANKGIGFALVRRLAQSELTVILTARDEAR 60

Query: 61  GLKAVEMLQNEGLNVLFRRLDVSDPVSIKAFAAWFIANFQALDILVNNAAVSFNDINENS 120
           GL+AVE L+NEGL+VLFRRLDVSDPVSI AFAAWF ANFQALDILVNNAAVSFN IN+NS
Sbjct: 61  GLQAVETLRNEGLHVLFRRLDVSDPVSISAFAAWFTANFQALDILVNNAAVSFNQINQNS 120

Query: 121 VENAETVIKTNFYGAKLLTEALLPFFRSSASRSRILNITSRLGTIDKVKNKKVKEILQSE 180
           VE+AETV+KTNFYGAKLLTEALLP+FRSS SRSRILNITSRLGTID+V+N KVKEILQS+
Sbjct: 121 VEHAETVLKTNFYGAKLLTEALLPYFRSSPSRSRILNITSRLGTIDRVRNMKVKEILQSK 180

Query: 181 DLAEEEIDGVVNAFLEDVKRGRWKKQGWPALWTDYAVSKLALNSYARVLAKRYGAFGSVS 240
           DLAEE+I GVV AFLEDV+RG W  QGWPA+WTDYAVSKLALNSY +VLA RYGAFGSVS
Sbjct: 181 DLAEEDIHGVVCAFLEDVQRGTWTNQGWPAVWTDYAVSKLALNSYTKVLANRYGAFGSVS 240

Query: 241 VNCFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFSWGPSYDTPRNSKL 300
           VNCFCPGFTQTSMT GKGTHTADAAALVGARLALLPP RLPTGQFFSWGP+Y TP+NSKL
Sbjct: 241 VNCFCPGFTQTSMTGGKGTHTADAAALVGARLALLPPHRLPTGQFFSWGPNYVTPKNSKL 300

BLAST of Tan0008492 vs. NCBI nr
Match: XP_022998381.1 ((+)-neomenthol dehydrogenase-like [Cucurbita maxima])

HSP 1 Score: 511.9 bits (1317), Expect = 3.7e-141
Identity = 262/300 (87.33%), Postives = 279/300 (93.00%), Query Frame = 0

Query: 1   MDSKEFDLSSPSSSPFSDRWWSNNSVAVVTGANKGIGFALVRRLAQLELTVILTARDEER 60
           M+SKEFDLSS SSSP SDRWWS N+VAVVTGANKGIGFALVRRLAQ ELTVILTARDE R
Sbjct: 1   MESKEFDLSS-SSSPVSDRWWSKNTVAVVTGANKGIGFALVRRLAQSELTVILTARDEAR 60

Query: 61  GLKAVEMLQNEGLNVLFRRLDVSDPVSIKAFAAWFIANFQALDILVNNAAVSFNDINENS 120
           GL+AVE L+NEGL+VLFRRLDVSDPVSI AFAAWF ANFQALDILVNNAAVSFNDIN+NS
Sbjct: 61  GLQAVETLRNEGLHVLFRRLDVSDPVSISAFAAWFTANFQALDILVNNAAVSFNDINQNS 120

Query: 121 VENAETVIKTNFYGAKLLTEALLPFFRSSASRSRILNITSRLGTIDKVKNKKVKEILQSE 180
           VE+AETV+KTNFYGAKLLTEALLP+F SS SRSRILNITSRLGTIDKV+N KVKEILQS+
Sbjct: 121 VEHAETVLKTNFYGAKLLTEALLPYFSSSPSRSRILNITSRLGTIDKVRNIKVKEILQSK 180

Query: 181 DLAEEEIDGVVNAFLEDVKRGRWKKQGWPALWTDYAVSKLALNSYARVLAKRYGAFGSVS 240
           DLAEE+I G+V AFLEDV+RG W  QGWPA+WTDYAVSKLALNSY RVLA RYGAFGSVS
Sbjct: 181 DLAEEDIHGIVCAFLEDVQRGTWTNQGWPAVWTDYAVSKLALNSYTRVLANRYGAFGSVS 240

Query: 241 VNCFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFSWGPSYDTPRNSKL 300
           VNCFCPGFTQTSMT GKGTHTADAAALVGARLALLPP RLPTGQFFSWGP+Y TP+NSKL
Sbjct: 241 VNCFCPGFTQTSMTGGKGTHTADAAALVGARLALLPPHRLPTGQFFSWGPNYVTPKNSKL 299

BLAST of Tan0008492 vs. NCBI nr
Match: XP_038891404.1 ((+)-neomenthol dehydrogenase-like [Benincasa hispida])

HSP 1 Score: 511.5 bits (1316), Expect = 4.8e-141
Identity = 258/300 (86.00%), Postives = 279/300 (93.00%), Query Frame = 0

Query: 1   MDSKEFDLSSPSSSPFSDRWWSNNSVAVVTGANKGIGFALVRRLAQLELTVILTARDEER 60
           M SKEFDL   SSSPFS+RWWS NSVA+VTGANKGIGFALVR+LAQ +LTVILTARDE R
Sbjct: 1   MASKEFDL---SSSPFSERWWSKNSVAIVTGANKGIGFALVRKLAQSDLTVILTARDEGR 60

Query: 61  GLKAVEMLQNEGLNVLFRRLDVSDPVSIKAFAAWFIANFQALDILVNNAAVSFNDINENS 120
           GLKAVE L+NEGL+VLFRRLDVSDPVSI AFAAWF +NFQALDILVNNAAVSFNDI+ENS
Sbjct: 61  GLKAVETLRNEGLHVLFRRLDVSDPVSINAFAAWFASNFQALDILVNNAAVSFNDIHENS 120

Query: 121 VENAETVIKTNFYGAKLLTEALLPFFRSSASRSRILNITSRLGTIDKVKNKKVKEILQSE 180
           VENAETV+KTNFYGAKLLTEALLP+FRSSAS+SRILNITSRLGT+DKV+N KVKEIL+SE
Sbjct: 121 VENAETVMKTNFYGAKLLTEALLPYFRSSASKSRILNITSRLGTVDKVRNMKVKEILESE 180

Query: 181 DLAEEEIDGVVNAFLEDVKRGRWKKQGWPALWTDYAVSKLALNSYARVLAKRYGAFGSVS 240
           D+ EE+I+GVVNAFL+DVKRG WKK GWP LWTDYAVSKLALN+Y RVLAKRYG +GSVS
Sbjct: 181 DVGEEDIEGVVNAFLKDVKRGTWKKAGWPTLWTDYAVSKLALNTYTRVLAKRYGEYGSVS 240

Query: 241 VNCFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFSWGPSYDTPRNSKL 300
           VN FCPGFTQTSMTRGKGTHTADAAALVGARLALLPP RLPTGQFF WGP+YD PRNSKL
Sbjct: 241 VNSFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPHRLPTGQFFFWGPNYDIPRNSKL 297

BLAST of Tan0008492 vs. ExPASy TrEMBL
Match: A0A6J1G196 ((+)-neomenthol dehydrogenase-like OS=Cucurbita moschata OX=3662 GN=LOC111449752 PE=3 SV=1)

HSP 1 Score: 513.8 bits (1322), Expect = 4.7e-142
Identity = 263/300 (87.67%), Postives = 280/300 (93.33%), Query Frame = 0

Query: 1   MDSKEFDLSSPSSSPFSDRWWSNNSVAVVTGANKGIGFALVRRLAQLELTVILTARDEER 60
           MDSKEFDLSS SSSPFSDRWWSNN+VAVVTGANKGIGFALVRRLAQ+ELTVILT R+E R
Sbjct: 1   MDSKEFDLSSASSSPFSDRWWSNNTVAVVTGANKGIGFALVRRLAQMELTVILTTRNEGR 60

Query: 61  GLKAVEMLQNEGLNVLFRRLDVSDPVSIKAFAAWFIANFQALDILVNNAAVSFNDINENS 120
           GLKAVE L+NEGLNVLFRRLDVSDP SI AFAAW +ANF ALDILVNNAAVSFNDI+ NS
Sbjct: 61  GLKAVESLRNEGLNVLFRRLDVSDPASINAFAAWVVANFPALDILVNNAAVSFNDIDHNS 120

Query: 121 VENAETVIKTNFYGAKLLTEALLPFFRSSASRSRILNITSRLGTIDKVKNKKVKEILQSE 180
           VENAETVIKTNFYGAKLLT+ALLP FRSS+SRSRILNITSRLGTIDKVKN+KVKEILQSE
Sbjct: 121 VENAETVIKTNFYGAKLLTQALLPLFRSSSSRSRILNITSRLGTIDKVKNEKVKEILQSE 180

Query: 181 DLAEEEIDGVVNAFLEDVKRGRWKKQGWPALWTDYAVSKLALNSYARVLAKRYGAFGSVS 240
           DLAEE+I+GVV+AFL+DVKRG WKK+GWPALWTDYAVSKLALN+Y RVLAKR     SVS
Sbjct: 181 DLAEEDIEGVVDAFLQDVKRGTWKKKGWPALWTDYAVSKLALNAYTRVLAKR---SRSVS 240

Query: 241 VNCFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFSWGPSYDTPRNSKL 300
           VN FCPGFTQTSMT GKGTHTADAAA+VGARLALLPP RLPTGQFFSWGP+YDTPRNSKL
Sbjct: 241 VNSFCPGFTQTSMTGGKGTHTADAAAVVGARLALLPPHRLPTGQFFSWGPNYDTPRNSKL 297

BLAST of Tan0008492 vs. ExPASy TrEMBL
Match: A0A6J1GZE8 ((+)-neomenthol dehydrogenase-like OS=Cucurbita moschata OX=3662 GN=LOC111458888 PE=3 SV=1)

HSP 1 Score: 512.7 bits (1319), Expect = 1.0e-141
Identity = 260/300 (86.67%), Postives = 278/300 (92.67%), Query Frame = 0

Query: 1   MDSKEFDLSSPSSSPFSDRWWSNNSVAVVTGANKGIGFALVRRLAQLELTVILTARDEER 60
           M+SKEFDLSS  SSP SDRWWS N+VAVVTGANKGIGFALVRRLAQ ELTVILTARDE R
Sbjct: 1   MESKEFDLSSSLSSPVSDRWWSKNTVAVVTGANKGIGFALVRRLAQSELTVILTARDEAR 60

Query: 61  GLKAVEMLQNEGLNVLFRRLDVSDPVSIKAFAAWFIANFQALDILVNNAAVSFNDINENS 120
           GL+AVE L+NEGL+VLFRRLDVSDPVSI AFAAWF ANFQALDILVNNAAVSFN IN+NS
Sbjct: 61  GLQAVETLRNEGLHVLFRRLDVSDPVSISAFAAWFTANFQALDILVNNAAVSFNQINQNS 120

Query: 121 VENAETVIKTNFYGAKLLTEALLPFFRSSASRSRILNITSRLGTIDKVKNKKVKEILQSE 180
           VE+AETV+KTNFYGAKLLTEALLP+FRSS SRSRILNITSRLGTID+V+N KVKEILQS+
Sbjct: 121 VEHAETVLKTNFYGAKLLTEALLPYFRSSPSRSRILNITSRLGTIDRVRNMKVKEILQSK 180

Query: 181 DLAEEEIDGVVNAFLEDVKRGRWKKQGWPALWTDYAVSKLALNSYARVLAKRYGAFGSVS 240
           DLAEE+I GVV AFLEDV+RG W  QGWPA+WTDYAVSKLALNSY +VLA RYGAFGSVS
Sbjct: 181 DLAEEDIHGVVCAFLEDVQRGTWTNQGWPAVWTDYAVSKLALNSYTKVLANRYGAFGSVS 240

Query: 241 VNCFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFSWGPSYDTPRNSKL 300
           VNCFCPGFTQTSMT GKGTHTADAAALVGARLALLPP RLPTGQFFSWGP+Y TP+NSKL
Sbjct: 241 VNCFCPGFTQTSMTGGKGTHTADAAALVGARLALLPPHRLPTGQFFSWGPNYVTPKNSKL 300

BLAST of Tan0008492 vs. ExPASy TrEMBL
Match: A0A6J1KA32 ((+)-neomenthol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111493032 PE=3 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 1.8e-141
Identity = 262/300 (87.33%), Postives = 279/300 (93.00%), Query Frame = 0

Query: 1   MDSKEFDLSSPSSSPFSDRWWSNNSVAVVTGANKGIGFALVRRLAQLELTVILTARDEER 60
           M+SKEFDLSS SSSP SDRWWS N+VAVVTGANKGIGFALVRRLAQ ELTVILTARDE R
Sbjct: 1   MESKEFDLSS-SSSPVSDRWWSKNTVAVVTGANKGIGFALVRRLAQSELTVILTARDEAR 60

Query: 61  GLKAVEMLQNEGLNVLFRRLDVSDPVSIKAFAAWFIANFQALDILVNNAAVSFNDINENS 120
           GL+AVE L+NEGL+VLFRRLDVSDPVSI AFAAWF ANFQALDILVNNAAVSFNDIN+NS
Sbjct: 61  GLQAVETLRNEGLHVLFRRLDVSDPVSISAFAAWFTANFQALDILVNNAAVSFNDINQNS 120

Query: 121 VENAETVIKTNFYGAKLLTEALLPFFRSSASRSRILNITSRLGTIDKVKNKKVKEILQSE 180
           VE+AETV+KTNFYGAKLLTEALLP+F SS SRSRILNITSRLGTIDKV+N KVKEILQS+
Sbjct: 121 VEHAETVLKTNFYGAKLLTEALLPYFSSSPSRSRILNITSRLGTIDKVRNIKVKEILQSK 180

Query: 181 DLAEEEIDGVVNAFLEDVKRGRWKKQGWPALWTDYAVSKLALNSYARVLAKRYGAFGSVS 240
           DLAEE+I G+V AFLEDV+RG W  QGWPA+WTDYAVSKLALNSY RVLA RYGAFGSVS
Sbjct: 181 DLAEEDIHGIVCAFLEDVQRGTWTNQGWPAVWTDYAVSKLALNSYTRVLANRYGAFGSVS 240

Query: 241 VNCFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFSWGPSYDTPRNSKL 300
           VNCFCPGFTQTSMT GKGTHTADAAALVGARLALLPP RLPTGQFFSWGP+Y TP+NSKL
Sbjct: 241 VNCFCPGFTQTSMTGGKGTHTADAAALVGARLALLPPHRLPTGQFFSWGPNYVTPKNSKL 299

BLAST of Tan0008492 vs. ExPASy TrEMBL
Match: A0A6J1HTC2 ((+)-neomenthol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111466403 PE=3 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 3.0e-141
Identity = 264/301 (87.71%), Postives = 280/301 (93.02%), Query Frame = 0

Query: 1   MDSKEFDLSSPSSSPFSDRWWSNNSVAVVTGANKGIGFALVRRLAQLELTVILTARDEER 60
           MDSKEFDLSS SSSPFSDRWWSNN+VAVVTGANKGIGFALVRRLAQ+ELTVILT R+E R
Sbjct: 1   MDSKEFDLSSASSSPFSDRWWSNNTVAVVTGANKGIGFALVRRLAQMELTVILTTRNEGR 60

Query: 61  GLKAVEMLQNEGLNVLFRRLDVSDPVSIKAFAAWFIANFQALDILVNNAAVSFNDINENS 120
           GLKAVE L+NEGLNV FRRLDVSDP SI AFAAWF+ANF ALDILVNNAAVSFNDI+ NS
Sbjct: 61  GLKAVESLRNEGLNVRFRRLDVSDPASINAFAAWFVANFPALDILVNNAAVSFNDIDHNS 120

Query: 121 VENAETVIKTNFYGAKLLTEALLPFFR-SSASRSRILNITSRLGTIDKVKNKKVKEILQS 180
           VENAETVIKTNFYGAKLLT+ALLP FR SS+SRSRILNITSRLGTIDKVKN+KVKEILQS
Sbjct: 121 VENAETVIKTNFYGAKLLTQALLPLFRSSSSSRSRILNITSRLGTIDKVKNEKVKEILQS 180

Query: 181 EDLAEEEIDGVVNAFLEDVKRGRWKKQGWPALWTDYAVSKLALNSYARVLAKRYGAFGSV 240
           EDLAEE+IDGVV+AFL+DVKRG WKK+GWPALWTDYAVSKLALN+Y RVLAKR     SV
Sbjct: 181 EDLAEEDIDGVVDAFLQDVKRGTWKKKGWPALWTDYAVSKLALNAYTRVLAKR---SRSV 240

Query: 241 SVNCFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFSWGPSYDTPRNSK 300
           SVN FCPGFTQTSMT GKGTHTADAAA+VGARLALLPP RLPTGQFFSWGP+YDTPRNSK
Sbjct: 241 SVNSFCPGFTQTSMTGGKGTHTADAAAVVGARLALLPPHRLPTGQFFSWGPNYDTPRNSK 298

BLAST of Tan0008492 vs. ExPASy TrEMBL
Match: A0A6J1DC35 ((+)-neomenthol dehydrogenase-like OS=Momordica charantia OX=3673 GN=LOC111019666 PE=3 SV=1)

HSP 1 Score: 494.6 bits (1272), Expect = 2.9e-136
Identity = 252/302 (83.44%), Postives = 276/302 (91.39%), Query Frame = 0

Query: 1   MDSKEFDLSSPSSSPFSDRWWSNNSVAVVTGANKGIGFALVRRLAQLELTVILTARDEER 60
           +DSKEFDLSSPSSSPFS RWWS N+VAVVTGANKGIGFALVRRLAQLELTVILTARD+ER
Sbjct: 14  LDSKEFDLSSPSSSPFSHRWWSKNTVAVVTGANKGIGFALVRRLAQLELTVILTARDQER 73

Query: 61  GLKAVEMLQNEGLNVLFRRLDVSDPVSIKAFAAWFIANFQALDILVNNAAVSFNDINENS 120
           G  AV+ L+NEGL VLFRRLDVSDP+SI +FAAWF ANFQALDILVNNAAVSFNDINENS
Sbjct: 74  GFNAVQTLRNEGLPVLFRRLDVSDPLSIASFAAWFTANFQALDILVNNAAVSFNDINENS 133

Query: 121 VENAETVIKTNFYGAKLLTEALLPFFRSSA-SRSRILNITSRLGTIDKVKNKKVKEILQS 180
           V NAETV+KTN+YGAKLLT+ALLP FRSSA SR+RILNITSRLGTIDKVKN KV+EIL S
Sbjct: 134 VANAETVMKTNYYGAKLLTQALLPAFRSSAPSRARILNITSRLGTIDKVKNVKVREILGS 193

Query: 181 EDLAEEEIDGVVNAFLEDVKRGRWKKQGWPALWTDYAVSKLALNSYARVLAKRYGAFG-S 240
           ED+ EE+I+GVVNAFL DVKRG WKK+GWPA+WT+YAVSKLALN+Y  VLAKR+  FG S
Sbjct: 194 EDVGEEDIEGVVNAFLGDVKRGTWKKEGWPAVWTEYAVSKLALNAYTAVLAKRFAQFGTS 253

Query: 241 VSVNCFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFSWGPSYDTPRNS 300
           VSVN FCPGFTQTSMTRGKGTHTA+AAA VGARLALLPP RLPTG FFSWGP+YD P+NS
Sbjct: 254 VSVNSFCPGFTQTSMTRGKGTHTAEAAAAVGARLALLPPHRLPTGHFFSWGPNYDVPKNS 313

BLAST of Tan0008492 vs. TAIR 10
Match: AT3G59710.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 334.3 bits (856), Expect = 9.8e-92
Identity = 168/268 (62.69%), Postives = 209/268 (77.99%), Query Frame = 0

Query: 20  WWSNNSVAVVTGANKGIGFALVRRLAQLELTVILTARDEERGLKAVEMLQNEGL-NVLFR 79
           WWS  + AVVTGANKGIGFA+V+RL +L LTV+LTAR+ E G +A E L+  G  NV F 
Sbjct: 24  WWSEETTAVVTGANKGIGFAVVKRLLELGLTVVLTARNAENGSQAAESLRRIGFGNVHFC 83

Query: 80  RLDVSDPVSIKAFAAWFIANFQALDILVNNAAVSFNDINENSVENAETVIKTNFYGAKLL 139
            LD+SDP SI AFA+WF  N   LDILVNNAAVSFN + EN ++  ET+IKTNFYGAKLL
Sbjct: 84  CLDISDPSSIAAFASWFGRNLGILDILVNNAAVSFNAVGENLIKEPETIIKTNFYGAKLL 143

Query: 140 TEALLPFFRSSASRSRILNITSRLGTIDKVKNKKVKEILQSEDLAEEEIDGVVNAFLEDV 199
           TEALLP FR S S SRILN++SRLGT++K+++  ++ IL+SEDL  E+ID  +  FL+DV
Sbjct: 144 TEALLPLFRRSVSVSRILNMSSRLGTLNKLRSPSIRRILESEDLTNEQIDATLTQFLQDV 203

Query: 200 KRGRWKKQGWPALWTDYAVSKLALNSYARVLAKRYGAFGSVSVNCFCPGFTQTSMTRGKG 259
           K G W+KQGWP  W DYA+SKLALN+Y+RVLA+RY     +SVNC CPGFT+TSMT G+G
Sbjct: 204 KSGTWEKQGWPENWPDYAISKLALNAYSRVLARRYDG-KKLSVNCLCPGFTRTSMTGGQG 263

Query: 260 THTADAAALVGARLALLPPQRLPTGQFF 287
           THTAD AA + A+L LLPP++L TG+F+
Sbjct: 264 THTADEAAAIVAKLVLLPPEKLATGKFY 290

BLAST of Tan0008492 vs. TAIR 10
Match: AT5G51030.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 239.6 bits (610), Expect = 3.3e-63
Identity = 131/296 (44.26%), Postives = 194/296 (65.54%), Query Frame = 0

Query: 2   DSKEFDLSSPSSSPFSD--RWWSNNSVAVVTGANKGIGFALVRRLAQLELTVILTARDEE 61
           D +  ++S   + P+SD  RWW++ +VAVVTGAN+GIGF +VR+LA   LTVILT+RDE 
Sbjct: 13  DKRLQEISLLRTIPYSDHQRWWTSETVAVVTGANRGIGFEMVRQLAGHGLTVILTSRDEN 72

Query: 62  RGLKAVEMLQNEGLNVLFRRLDVSDPVSIKAFAAWFIANFQALDILVNNAAVSFNDINEN 121
            G++A ++LQ  G NV F RLD+ D  SI+ F  W    +  +D+L+NNA V++N  ++N
Sbjct: 73  VGVEAAKILQEGGFNVDFHRLDILDSSSIQEFCEWIKEKYGFIDVLINNAGVNYNVGSDN 132

Query: 122 SVENAETVIKTNFYGAKLLTEALLPFFRSSASRSRILNITSRLGTI----DKVKNKKVK- 181
           SVE +  VI TN+YG K +  A++P  R +   +RI+N+TSRLG +     K++N+ V+ 
Sbjct: 133 SVEFSHMVISTNYYGTKNIINAMIPLMRHACQGARIVNVTSRLGRLKGRHSKLENEDVRA 192

Query: 182 EILQSEDLAEEEIDGVVNAFLEDVKRGRWKKQGWPALWTDYAVSKLALNSYARVLAKRYG 241
           +++  + L EE +D  V+ FL+ V+ G W+  GWP  +TDY+VSK+A+N+Y RVLAK   
Sbjct: 193 KLMDVDSLTEEIVDKTVSEFLKQVEEGTWESGGWPHSFTDYSVSKMAVNAYTRVLAKELS 252

Query: 242 ---AFGSVSVNCFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFS 288
                  +  NCFCPG+ +T+MT   G  +A+  A  G  LALLP Q + TG+FF+
Sbjct: 253 ERPEGEKIYANCFCPGWVKTAMTGYAGNVSAEDGADTGVWLALLPDQAI-TGKFFA 307

BLAST of Tan0008492 vs. TAIR 10
Match: AT5G61830.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 234.6 bits (597), Expect = 1.1e-61
Identity = 133/293 (45.39%), Postives = 192/293 (65.53%), Query Frame = 0

Query: 7   DLSSPSSSPFSD--RWWSNNSVAVVTGANKGIGFALVRRLAQLELTVILTARDEERGLKA 66
           ++S   + P+SD  RWWS  +VAVVTG+N+GIGF + R+LA   LTV+LTAR+   GL+A
Sbjct: 17  EISLLRTIPYSDHHRWWSCENVAVVTGSNRGIGFEIARQLAVHGLTVVLTARNVNAGLEA 76

Query: 67  VEML--QNEGLNVLFRRLDVSDPVSIKAFAAWFIANFQALDILVNNAAVSFNDINENSVE 126
           V+ L  Q EGL V F +LDV+D  SI+ F  W    F  LDILVNNA V++N  ++N+VE
Sbjct: 77  VKSLRHQEEGLKVYFHQLDVTDSSSIREFGCWLKQTFGGLDILVNNAGVNYNLGSDNTVE 136

Query: 127 NAETVIKTNFYGAKLLTEALLPFFRSSASRSRILNITSRLGTI----DKVKNKKVKEILQ 186
            AETVI TN+ G K +T+A++P  R S   +R++N++SRLG +    +++ N ++++ L 
Sbjct: 137 FAETVISTNYQGTKNMTKAMIPLMRPSPHGARVVNVSSRLGRVNGRRNRLANVELRDQLS 196

Query: 187 SEDLAEEE-IDGVVNAFLEDVKRGRWKKQGWPALWTDYAVSKLALNSYARVLAK---RYG 246
           S DL  EE ID  V+ F+  VK G W+  GWP  +TDY++SKLA+N+Y R++AK   R G
Sbjct: 197 SPDLLTEELIDRTVSKFINQVKDGTWESGGWPQTFTDYSMSKLAVNAYTRLMAKELERRG 256

Query: 247 AFGSVSVNCFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFS 288
               + VN FCPG+ +T+MT   G    + AA  G  L+L+  +   TG+FF+
Sbjct: 257 EEEKIYVNSFCPGWVKTAMTGYAGNMPPEDAADTGVWLSLVLSEEAVTGKFFA 309

BLAST of Tan0008492 vs. TAIR 10
Match: AT3G61220.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 192.6 bits (488), Expect = 4.6e-49
Identity = 120/285 (42.11%), Postives = 162/285 (56.84%), Query Frame = 0

Query: 27  AVVTGANKGIGFALVRRLAQLELTVILTARDEERGLKAVEMLQNE----GLNVLFRRLDV 86
           AVVTGAN+GIGF + R+LA   + V+LT+RDE RGL+AVE L+ E      ++LF +LDV
Sbjct: 9   AVVTGANRGIGFEICRQLASEGIRVVLTSRDENRGLEAVETLKKELEISDQSLLFHQLDV 68

Query: 87  SDPVSIKAFAAWFIANFQALDILVNNAAV-------------------SFNDINENSVEN 146
           +DP SI + A +    F  LDILVNNA +                    +++I   + E 
Sbjct: 69  ADPASITSLAEFVKTQFGKLDILVNNAGIGGIITDAEALRAGAGKEGFKWDEIITETYEL 128

Query: 147 AETVIKTNFYGAKLLTEALLPFFRSSASRSRILNITSRLGTIDKVKNKKVKEIL-QSEDL 206
            E  IK N+YG K + EA +P  + S S  RI+N++S +G +  V N+  K IL  +E+L
Sbjct: 129 TEECIKINYYGPKRMCEAFIPLLKLSDS-PRIVNVSSSMGQLKNVLNEWAKGILSDAENL 188

Query: 207 AEEEIDGVVNAFLEDVKRGRWKKQGWPALWTDYAVSKLALNSYARVLAKRYGAFGSVSVN 266
            EE ID V+N  L D K G  K++ W    + Y VSK +LN Y RVLAK++  F    VN
Sbjct: 189 TEERIDQVINQLLNDFKEGTVKEKNWAKFMSAYVVSKASLNGYTRVLAKKHPEF---RVN 248

Query: 267 CFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFS 288
             CPGF +T M    G  + +  A    RLALLP Q  P+G FFS
Sbjct: 249 AVCPGFVKTDMNFKTGVLSVEEGASSPVRLALLPHQETPSGCFFS 289

BLAST of Tan0008492 vs. TAIR 10
Match: AT3G61220.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 192.6 bits (488), Expect = 4.6e-49
Identity = 120/285 (42.11%), Postives = 162/285 (56.84%), Query Frame = 0

Query: 27  AVVTGANKGIGFALVRRLAQLELTVILTARDEERGLKAVEMLQNE----GLNVLFRRLDV 86
           AVVTGAN+GIGF + R+LA   + V+LT+RDE RGL+AVE L+ E      ++LF +LDV
Sbjct: 16  AVVTGANRGIGFEICRQLASEGIRVVLTSRDENRGLEAVETLKKELEISDQSLLFHQLDV 75

Query: 87  SDPVSIKAFAAWFIANFQALDILVNNAAV-------------------SFNDINENSVEN 146
           +DP SI + A +    F  LDILVNNA +                    +++I   + E 
Sbjct: 76  ADPASITSLAEFVKTQFGKLDILVNNAGIGGIITDAEALRAGAGKEGFKWDEIITETYEL 135

Query: 147 AETVIKTNFYGAKLLTEALLPFFRSSASRSRILNITSRLGTIDKVKNKKVKEIL-QSEDL 206
            E  IK N+YG K + EA +P  + S S  RI+N++S +G +  V N+  K IL  +E+L
Sbjct: 136 TEECIKINYYGPKRMCEAFIPLLKLSDS-PRIVNVSSSMGQLKNVLNEWAKGILSDAENL 195

Query: 207 AEEEIDGVVNAFLEDVKRGRWKKQGWPALWTDYAVSKLALNSYARVLAKRYGAFGSVSVN 266
            EE ID V+N  L D K G  K++ W    + Y VSK +LN Y RVLAK++  F    VN
Sbjct: 196 TEERIDQVINQLLNDFKEGTVKEKNWAKFMSAYVVSKASLNGYTRVLAKKHPEF---RVN 255

Query: 267 CFCPGFTQTSMTRGKGTHTADAAALVGARLALLPPQRLPTGQFFS 288
             CPGF +T M    G  + +  A    RLALLP Q  P+G FFS
Sbjct: 256 AVCPGFVKTDMNFKTGVLSVEEGASSPVRLALLPHQETPSGCFFS 296

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M2E26.5e-4842.11(+)-neomenthol dehydrogenase OS=Arabidopsis thaliana OX=3702 GN=SDR1 PE=1 SV=1[more]
A4UHT73.0e-4542.18Salutaridine reductase OS=Papaver bracteatum OX=215227 GN=SALR PE=1 SV=1[more]
Q9ZUH53.3e-4439.44Short-chain dehydrogenase/reductase 2b OS=Arabidopsis thaliana OX=3702 GN=SDR2b ... [more]
Q6WAU12.0e-4138.82(-)-isopiperitenone reductase OS=Mentha piperita OX=34256 PE=1 SV=1[more]
B2X0501.7e-4037.54(+)-neomenthol dehydrogenase OS=Capsicum annuum OX=4072 GN=MNR1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
KAG6573598.19.7e-14287.67(+)-neomenthol dehydrogenase, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
XP_022945555.19.7e-14287.67(+)-neomenthol dehydrogenase-like [Cucurbita moschata][more]
XP_022957511.12.2e-14186.67(+)-neomenthol dehydrogenase-like [Cucurbita moschata][more]
XP_022998381.13.7e-14187.33(+)-neomenthol dehydrogenase-like [Cucurbita maxima][more]
XP_038891404.14.8e-14186.00(+)-neomenthol dehydrogenase-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1G1964.7e-14287.67(+)-neomenthol dehydrogenase-like OS=Cucurbita moschata OX=3662 GN=LOC111449752 ... [more]
A0A6J1GZE81.0e-14186.67(+)-neomenthol dehydrogenase-like OS=Cucurbita moschata OX=3662 GN=LOC111458888 ... [more]
A0A6J1KA321.8e-14187.33(+)-neomenthol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111493032 PE... [more]
A0A6J1HTC23.0e-14187.71(+)-neomenthol dehydrogenase-like OS=Cucurbita maxima OX=3661 GN=LOC111466403 PE... [more]
A0A6J1DC352.9e-13683.44(+)-neomenthol dehydrogenase-like OS=Momordica charantia OX=3673 GN=LOC111019666... [more]
Match NameE-valueIdentityDescription
AT3G59710.19.8e-9262.69NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G51030.13.3e-6344.26NAD(P)-binding Rossmann-fold superfamily protein [more]
AT5G61830.11.1e-6145.39NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G61220.14.6e-4942.11NAD(P)-binding Rossmann-fold superfamily protein [more]
AT3G61220.24.6e-4942.11NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 153..161
score: 41.05
coord: 100..111
score: 45.96
coord: 215..234
score: 29.71
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 26..43
score: 49.75
coord: 237..254
score: 32.94
coord: 100..111
score: 49.24
coord: 215..234
score: 29.52
coord: 147..163
score: 24.43
IPR002347Short-chain dehydrogenase/reductase SDRPFAMPF00106adh_shortcoord: 26..164
e-value: 6.3E-30
score: 104.1
coord: 211..257
e-value: 8.7E-7
score: 28.6
NoneNo IPR availableGENE3D3.40.50.720coord: 21..292
e-value: 2.2E-76
score: 258.7
NoneNo IPR availablePANTHERPTHR43490:SF60NAD(P)-BINDING ROSSMANN-FOLD SUPERFAMILY PROTEINcoord: 20..290
NoneNo IPR availablePANTHERPTHR43490(+)-NEOMENTHOL DEHYDROGENASEcoord: 20..290
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 25..289

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0008492.1Tan0008492.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity