Tan0008479 (gene) Snake gourd v1

Overview


Name	Tan0008479
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	protein NUCLEAR FUSION DEFECTIVE 4-like
Location	LG07: 23200880 .. 23202622 (-)
RNA-Seq Expression	Tan0008479
Synteny	Tan0008479

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSexonpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTAATAATCGAAGATCAGAAATTTGGGGTTTCATCAAACAAGTAGTAGGAGGACGATGGTTTTCAGTATTTGCGTCGTTTTTGTTAATGATCGGCAACGGTACAACTTACATATATGGAACATACTCAAAAGTGATAAAGACCCAATTTGATTACAATCAAACCCAAATTAACACATTGGGATTCGCCAAAGATTTGGGGTCCAACGTTGGAATTTTGGCTGGTCTTCTTGCCGAGGTTGTTCCTTTGTGGGTTCTGTTGTTGGTCGGTGGATTTCTCAACTTCATCAGCTACTTCATGATTTGGCTCTCCATCACTCACCGTATTGCCAAGCCTCAATTTTGGCAAATGTTTCTTTACATTTGTTTGGCTGCTAATTCTGCAAATTTCTCTAATACTGCTATTTTGGTCACTAGCGTTAGAAATTTCCCTGATCGACGAGGCATTATTTTGGGTCTTATTAAAGGCTTTGTGGGTATTGCTGGTGCTATTTTAACCCAATTTTATTTAGCCATTTATGGCCATGAAGACCCATCTAATCAAATTCTTCTTTTCGCTTGGCTTCCTTCTACCATAATCCTTATTTTTTTCAAGTCAATTCGACCGATTAATGTTCGTAAACAACCCGACGAGCTCACAGTGTTTTACCATTTGCTTTATGTCTCCATGCTACTGGCAATTTTCCTCTTGTTTTTAACTCTTGCACAAAAACAAGTCACTTTCTCTCATGTTGGGTTCATGAGTGGTGCGTTGGTTATCGGTTGCTTGTTATGCCTTCCCCTGTTAATCGCATTTAGAGAAGAATACTTCCTCTACAAACACAACAATGACATAGATTCTTCTTCATCTGTCACTGTCTCCATTGTCGACAATCAAAAGCCTCCATTATTGGCGGTCCCTGAAAAACCAACCGAAGTTGTCGAGAATTCACCCTCGTGTTTCTCAAACATCATGAATAAACCAGCAAGAGGGGAAGATTTCACTATTTTTCAAGCTATGTTTAGCAAAGATATGGCTTTGATTTGTTTAGCTACTTTTTCTGGATGTGGATCTTCTCTTGCCGCCATTGATAATTTAGGCCAAATTGGCGAATCATTGGGCTATCCACAACAAGCCATAAGCATCTTCGTTTCTTGGATTTCTATTTTCAATTTCTTCGGTCGAGTCTTCTCTGGTTTCATCTCTGAGACTCTTATGCTCAAATACAAATTACCTCGTCCAGTAATTTTCTCCTTTGCCTTTCTCGTCACTTGCCTTGGCCAACTTTGTATCGCCTATCCATCCATTGGTTCCGTCTACATCGCTTCGTTGATTATCGGGTTCGGATTTGGAGCTCAAGTTCCATTGCTATTTGCTATAATCTCAGAGCTTTTTGGGCTCAAACATTACTCAACTTTGTTCAATTGGGGGCAATTGATAGTCCCATTTGGATCTTATATTCTAAATGTGGATGTAGTTGGGAAATTGTACGATGTGGAAGCTTTACGTGAAGGTTTGAATAATAATAATATTAAGTTGGGGAAGGCAATTACTTGTATGGGAGCTCATTGCTTTAGTGGCTCTTTCACAATTTTGGCTTCGGTCACATTGTTTGCTGCTTTGACAATGCTTGTGTTGGCTTATAGGACTCGAGATTTTTACAAAGGAGATGTATATAACAAGTATAAAGAGGACTTGTGGATTCCACAATCCGAAATGGAGTTTTATCGTTTAGATAACAGGAAGAGGATTGAAGAATAA

mRNA sequence

ATGAGTAATAATCGAAGATCAGAAATTTGGGGTTTCATCAAACAAGTAGTAGGAGGACGATGGTTTTCAGTATTTGCGTCGTTTTTGTTAATGATCGGCAACGGTACAACTTACATATATGGAACATACTCAAAAGTGATAAAGACCCAATTTGATTACAATCAAACCCAAATTAACACATTGGGATTCGCCAAAGATTTGGGGTCCAACGTTGGAATTTTGGCTGGTCTTCTTGCCGAGGTTGTTCCTTTGTGGGTTCTGTTGTTGGTCGGTGGATTTCTCAACTTCATCAGCTACTTCATGATTTGGCTCTCCATCACTCACCGTATTGCCAAGCCTCAATTTTGGCAAATGTTTCTTTACATTTGTTTGGCTGCTAATTCTGCAAATTTCTCTAATACTGCTATTTTGGTCACTAGCGTTAGAAATTTCCCTGATCGACGAGGCATTATTTTGGGTCTTATTAAAGGCTTTGTGGGTATTGCTGGTGCTATTTTAACCCAATTTTATTTAGCCATTTATGGCCATGAAGACCCATCTAATCAAATTCTTCTTTTCGCTTGGCTTCCTTCTACCATAATCCTTATTTTTTTCAAGTCAATTCGACCGATTAATGTTCGTAAACAACCCGACGAGCTCACAGTGTTTTACCATTTGCTTTATGTCTCCATGCTACTGGCAATTTTCCTCTTGTTTTTAACTCTTGCACAAAAACAAGTCACTTTCTCTCATGTTGGGTTCATGAGTGGTGCGTTGGTTATCGGTTGCTTGTTATGCCTTCCCCTGTTAATCGCATTTAGAGAAGAATACTTCCTCTACAAACACAACAATGACATAGATTCTTCTTCATCTGTCACTGTCTCCATTGTCGACAATCAAAAGCCTCCATTATTGGCGGTCCCTGAAAAACCAACCGAAGTTGTCGAGAATTCACCCTCGTGTTTCTCAAACATCATGAATAAACCAGCAAGAGGGGAAGATTTCACTATTTTTCAAGCTATGTTTAGCAAAGATATGGCTTTGATTTGTTTAGCTACTTTTTCTGGATGTGGATCTTCTCTTGCCGCCATTGATAATTTAGGCCAAATTGGCGAATCATTGGGCTATCCACAACAAGCCATAAGCATCTTCGTTTCTTGGATTTCTATTTTCAATTTCTTCGGTCGAGTCTTCTCTGGTTTCATCTCTGAGACTCTTATGCTCAAATACAAATTACCTCGTCCAGTAATTTTCTCCTTTGCCTTTCTCGTCACTTGCCTTGGCCAACTTTGTATCGCCTATCCATCCATTGGTTCCGTCTACATCGCTTCGTTGATTATCGGGTTCGGATTTGGAGCTCAAGTTCCATTGCTATTTGCTATAATCTCAGAGCTTTTTGGGCTCAAACATTACTCAACTTTGTTCAATTGGGGGCAATTGATAGTCCCATTTGGATCTTATATTCTAAATGTGGATGTAGTTGGGAAATTGTACGATGTGGAAGCTTTACGTGAAGGTTTGAATAATAATAATATTAAGTTGGGGAAGGCAATTACTTGTATGGGAGCTCATTGCTTTAGTGGCTCTTTCACAATTTTGGCTTCGGTCACATTGTTTGCTGCTTTGACAATGCTTGTGTTGGCTTATAGGACTCGAGATTTTTACAAAGGAGATGTATATAACAAGTATAAAGAGGACTTGTGGATTCCACAATCCGAAATGGAGTTTTATCGTTTAGATAACAGGAAGAGGATTGAAGAATAA

Coding sequence (CDS)

ATGAGTAATAATCGAAGATCAGAAATTTGGGGTTTCATCAAACAAGTAGTAGGAGGACGATGGTTTTCAGTATTTGCGTCGTTTTTGTTAATGATCGGCAACGGTACAACTTACATATATGGAACATACTCAAAAGTGATAAAGACCCAATTTGATTACAATCAAACCCAAATTAACACATTGGGATTCGCCAAAGATTTGGGGTCCAACGTTGGAATTTTGGCTGGTCTTCTTGCCGAGGTTGTTCCTTTGTGGGTTCTGTTGTTGGTCGGTGGATTTCTCAACTTCATCAGCTACTTCATGATTTGGCTCTCCATCACTCACCGTATTGCCAAGCCTCAATTTTGGCAAATGTTTCTTTACATTTGTTTGGCTGCTAATTCTGCAAATTTCTCTAATACTGCTATTTTGGTCACTAGCGTTAGAAATTTCCCTGATCGACGAGGCATTATTTTGGGTCTTATTAAAGGCTTTGTGGGTATTGCTGGTGCTATTTTAACCCAATTTTATTTAGCCATTTATGGCCATGAAGACCCATCTAATCAAATTCTTCTTTTCGCTTGGCTTCCTTCTACCATAATCCTTATTTTTTTCAAGTCAATTCGACCGATTAATGTTCGTAAACAACCCGACGAGCTCACAGTGTTTTACCATTTGCTTTATGTCTCCATGCTACTGGCAATTTTCCTCTTGTTTTTAACTCTTGCACAAAAACAAGTCACTTTCTCTCATGTTGGGTTCATGAGTGGTGCGTTGGTTATCGGTTGCTTGTTATGCCTTCCCCTGTTAATCGCATTTAGAGAAGAATACTTCCTCTACAAACACAACAATGACATAGATTCTTCTTCATCTGTCACTGTCTCCATTGTCGACAATCAAAAGCCTCCATTATTGGCGGTCCCTGAAAAACCAACCGAAGTTGTCGAGAATTCACCCTCGTGTTTCTCAAACATCATGAATAAACCAGCAAGAGGGGAAGATTTCACTATTTTTCAAGCTATGTTTAGCAAAGATATGGCTTTGATTTGTTTAGCTACTTTTTCTGGATGTGGATCTTCTCTTGCCGCCATTGATAATTTAGGCCAAATTGGCGAATCATTGGGCTATCCACAACAAGCCATAAGCATCTTCGTTTCTTGGATTTCTATTTTCAATTTCTTCGGTCGAGTCTTCTCTGGTTTCATCTCTGAGACTCTTATGCTCAAATACAAATTACCTCGTCCAGTAATTTTCTCCTTTGCCTTTCTCGTCACTTGCCTTGGCCAACTTTGTATCGCCTATCCATCCATTGGTTCCGTCTACATCGCTTCGTTGATTATCGGGTTCGGATTTGGAGCTCAAGTTCCATTGCTATTTGCTATAATCTCAGAGCTTTTTGGGCTCAAACATTACTCAACTTTGTTCAATTGGGGGCAATTGATAGTCCCATTTGGATCTTATATTCTAAATGTGGATGTAGTTGGGAAATTGTACGATGTGGAAGCTTTACGTGAAGGTTTGAATAATAATAATATTAAGTTGGGGAAGGCAATTACTTGTATGGGAGCTCATTGCTTTAGTGGCTCTTTCACAATTTTGGCTTCGGTCACATTGTTTGCTGCTTTGACAATGCTTGTGTTGGCTTATAGGACTCGAGATTTTTACAAAGGAGATGTATATAACAAGTATAAAGAGGACTTGTGGATTCCACAATCCGAAATGGAGTTTTATCGTTTAGATAACAGGAAGAGGATTGAAGAATAA

Protein sequence

MSNNRRSEIWGFIKQVVGGRWFSVFASFLLMIGNGTTYIYGTYSKVIKTQFDYNQTQINTLGFAKDLGSNVGILAGLLAEVVPLWVLLLVGGFLNFISYFMIWLSITHRIAKPQFWQMFLYICLAANSANFSNTAILVTSVRNFPDRRGIILGLIKGFVGIAGAILTQFYLAIYGHEDPSNQILLFAWLPSTIILIFFKSIRPINVRKQPDELTVFYHLLYVSMLLAIFLLFLTLAQKQVTFSHVGFMSGALVIGCLLCLPLLIAFREEYFLYKHNNDIDSSSSVTVSIVDNQKPPLLAVPEKPTEVVENSPSCFSNIMNKPARGEDFTIFQAMFSKDMALICLATFSGCGSSLAAIDNLGQIGESLGYPQQAISIFVSWISIFNFFGRVFSGFISETLMLKYKLPRPVIFSFAFLVTCLGQLCIAYPSIGSVYIASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNWGQLIVPFGSYILNVDVVGKLYDVEALREGLNNNNIKLGKAITCMGAHCFSGSFTILASVTLFAALTMLVLAYRTRDFYKGDVYNKYKEDLWIPQSEMEFYRLDNRKRIEE

Homology

[Close]

BLAST of Tan0008479 vs. ExPASy Swiss-Prot
Match: F4I9E1 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 8.5e-28
Identity = 135/539 (25.05%), Postives = 240/539 (44.53%), Query Frame = 0


Query: 20  RWFSVFASFLLMIGNGTTYIYGTYSKVIKTQFDYNQTQINTLGFAKDLGSNVGILAGLLA 79
           +W  + A+  +    GT + +  YS  +K+    +Q ++N L  A DLG   G  +G+  
Sbjct: 43  KWTVLVAAIWIQASTGTNFDFSAYSSHLKSVLGISQVRLNYLAVASDLGKAFGWSSGIAL 102

Query: 80  EVVPLWVLLLVGGFLNFISYFMIWLSITHRIAKPQFWQMFLYICLAANSANFSNTAILVT 139
              PL V+L     + F+ Y + WL IT+ I  P +  +FL   LA  S  + NTA  + 
Sbjct: 103 GYFPLSVVLFAAAAMGFVGYGVQWLVITNIITLP-YSLVFLCCLLAGLSICWFNTACFIL 162

Query: 140 SVRNFPDRRGIILGLIKGFVGIAGAILTQFYLAIYGHEDPSNQILLFAWLPSTIILIFFK 199
            +R+FP+ R + L L   F GI+ A+ +  + AI    +PS+  L         +++ F 
Sbjct: 163 CIRHFPNNRALALSLTVSFNGISAALYSLAFNAI----NPSSSNLYLLLNSLVPLVVSFA 222

Query: 200 SIRPINVRKQPDELTVF------YHLLYVSMLLAIFLLF-LTLAQKQVTFSHVGFMSGAL 259
           ++ P+  +   D    +       H+  +  +LA+   F L L+    + + + F+   +
Sbjct: 223 ALYPVLTKPSLDTTPDYDSRRHDSHVFTILNVLAVITSFHLLLSSSSTSSARLNFIGAVV 282

Query: 260 VIGCLLCLPLLIAFREEYFLYKHNNDIDSSSSVTVSI-VDNQKPPLLAVPEKPTEVVENS 319
           ++   LC PLL+ +  +YFL   N  ++  SS  V + +D  K    +V  K     E+ 
Sbjct: 283 LLVFPLCAPLLV-YARDYFLPVINARLNHESSGYVMLNIDELKNQKTSVSSKTG--YEHM 342

Query: 320 PSCFSNIMNKPARGEDFTIFQAMFSKDMALICLATFSGCGSSLAAIDNLGQIGESLGYPQ 379
            +      N    G++ +    +   +  L  +A F G    L   +NLGQI +SLG   
Sbjct: 343 GTAKEG--NTVRLGDEHSFRLLISRLEFWLYYIAYFCGGTIGLVYSNNLGQIAQSLG--- 402

Query: 380 QAISIFVSWISIFNFFGRVFSGFISETLMLKYKLPRPVIFSFAFLVTCLGQLCIAYPSI- 439
           Q  +  V+  S F+FFGR+ S    + +  +++L R   F+ A L T +    +A  S  
Sbjct: 403 QNSTTLVTIYSSFSFFGRLLSA-APDFMHKRFRLTRTGWFAIALLPTPIAFFLLAVSSSQ 462

Query: 440 -GSVYIASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNWGQLIVPFGSYILNVDVVGK 499
             ++  A+ +IG   G       +I S+LFG        N     +P GS +    +   
Sbjct: 463 QTALQTATALIGLSSGFIFAAAVSITSDLFGPNSVGVNHNILITNIPIGSLLYGY-IAAS 522

Query: 500 LYDVEALREGLNNNNIKLGKAITCMGAHCFSGSFTILASVTLFAALTMLVLAYRTRDFY 549
           +Y+  A  +        +  +I C+G  C+  +F     +++   ++ L L  RT+  Y
Sbjct: 523 IYEANASPD----ITPIVSDSIVCIGRDCYFKTFVFWGCLSILGVVSSLSLYIRTKPVY 562

[Close]

BLAST of Tan0008479 vs. ExPASy Swiss-Prot
Match: Q03795 (Uncharacterized membrane protein YMR155W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=YMR155W PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 1.7e-04
Identity = 126/565 (22.30%), Postives = 224/565 (39.65%), Query Frame = 0


Query: 10  WGFIKQVVGGRWFSVFASFLLMIGNGTTYIYGTYSKVIKTQFDYNQTQINTLGFAKDLGS 69
           +G +K  VGG         ++ +G GT Y++  Y+  + ++     +  + L F+  +GS
Sbjct: 16  FGHLKSFVGGN--------VVALGAGTPYLFSFYAPQLLSKCHIPVSASSKLSFSLTIGS 75

Query: 70  N-VGILAGLLAEVVPLWVLLLVGGFLNFISYFMIWLSITHRIAKPQFWQMFLYICLAANS 129
           + +GILAG++ +  P  +  L+G    FI+Y ++ L   H       W     I L+   
Sbjct: 76  SLMGILAGIVVDRSPK-LSCLIGSMCVFIAYLILNLCYKHE------WSSTFLISLSLVL 135

Query: 130 ANFSNTAILVTSVR----NFPDRRGIILGLIKGFVGIAGAILTQFYLAIYGHEDPSNQIL 189
             + + +    SV+    NFP  RG          G++G + +     ++G       I 
Sbjct: 136 IGYGSVSGFYASVKCANTNFPQHRGTAGAFPVSLYGLSGMVFSYLCSKLFGENIEHVFIF 195

Query: 190 LFAWLPSTIILIFFKSIRPINVRKQPDELTVFYHLLYVSMLLAIFLLFLTLAQKQVTFSH 249
           L       I++ +F     I    + D+ ++    L  S      ++ L         S 
Sbjct: 196 LMVACGCMILVGYFS--LDIFSNAEGDDASIKEWELQKSRETDDNIVPLYENSNDYIGSP 255

Query: 250 VGFMSGALVIGCLLCLPLLIAFREEYFLYKHNNDIDSSSSVTVSIVDNQ--KPPLLAVPE 309
           V   S A                E Y L    +D    +S   ++ D Q    PLL+   
Sbjct: 256 VRSSSPATY--------------ETYAL----SDNFQETSEFFALEDRQLSNRPLLSPSS 315

Query: 310 KPTEVVENSPSCFSNIM--NKPARGEDFTIFQAMFSKDMA--LICLATFSGCGSSLAAID 369
             T+      +   N +  N   +     +FQ++ S       I L    G G  L  I 
Sbjct: 316 PHTKYDFEDENTSKNTVGENSAQKSMRLHVFQSLKSSTFIGYYIVLGILQGVG--LMYIY 375

Query: 370 NLG-QIGESLGYP---------QQAISIFVSWISIFNFFGRVFSGFISETLMLKYKLPRP 429
           ++G  +   +  P         ++  S+ V+ +S+ +F GR+ SG IS+ L+ K+K  R 
Sbjct: 376 SVGFMVQAQVSTPPLNQLPINAEKIQSLQVTLLSLLSFCGRLSSGPISDFLVKKFKAQRL 435

Query: 430 VIFSFAFLVTCLGQLCIAY-------------PSIGSVYIASLIIGFGFGAQVPLLFAII 489
                A L+  L    I++              S  ++ + S I G+ FG       +I+
Sbjct: 436 WNIVIASLLVFLASNKISHDFSSIEDPSLRASKSFKNISVCSAIFGYSFGVLFGTFPSIV 495

Query: 490 SELFGLKHYSTLFNWGQLIVPFGSYILNV--DVVGKLYDVEALREGLNNNNIKLGKAITC 539
           ++ FG   YSTL  WG ++   G + ++V  D++G+  D +A   G ++ N K       
Sbjct: 496 ADRFGTNGYSTL--WG-VLTTGGVFSVSVFTDILGR--DFKA-NTGDDDGNCK------- 530

[Close]

BLAST of Tan0008479 vs. NCBI nr
Match: XP_022970365.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima] >XP_022970399.1 protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 832.0 bits (2148), Expect = 3.1e-237
Identity = 419/580 (72.24%), Postives = 485/580 (83.62%), Query Frame = 0


Query: 1   MSNNRRSEIWGFIKQVVGGRWFSVFASFLLMIGNGTTYIYGTYSKVIKTQFDYNQTQINT 60
           M N   SE WGFIKQVV GRWFSVFA  ++MIGNGTTY++ TYSKVIKTQFDY+QTQINT
Sbjct: 1   MGNKGGSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNVGILAGLLAEVVPLWVLLLVGGFLNFISYFMIWLSITHRIAKPQFWQMFL 120
           LGFAKDLGSN+GI+AGLLAEV P WVL ++G F NFI +F+IWLSIT RIAKP+FWQMF+
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICLAANSANFSNTAILVTSVRNFPDRRGIILGLIKGFVGIAGAILTQFYLAIYGHEDPS 180
            +C   NS+N++NTAI+VTSVRNFPDRRGIILGL+KG+VGI GAI++QFYLAIYGH DPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPS 180

Query: 181 NQILLFAWLPSTIILIFFKSIRPINVRKQPDELTVFYHLLYVSMLLAIFLLFLTLAQKQV 240
           N +LLFAWLPS +ILI F SIRPI +RK P+EL VFY LLYVS+LLAIF+LFLT+AQKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 TFSHVGFMSGALVIGCLLCLPLLIAFREEYFLYKHNNDIDSSSSVTVSIVDNQKPPLLAV 300
            FS  G++ GA  +  LLC+P+LIA REE  LYK N    +  SVTVS+V +Q PP L  
Sbjct: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQ-TAGPSVTVSMV-HQNPPTLPA 300

Query: 301 PEKPTEVVENSPSCFSNIMNKPARGEDFTIFQAMFSKDMALICLATFSGCGSSLAAIDNL 360
               +E+ E SPSC  NI NKP RGEDFTI QA+FSKDMALICLATFSGCGSSLAAIDNL
Sbjct: 301 -SADSEIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNL 360

Query: 361 GQIGESLGYPQQAISIFVSWISIFNFFGRVFSGFISETLMLKYKLPRPVIFSFAFLVTCL 420
           GQ+GESLGYPQ+AI I VSW+SIFNFFGRVFSGFISETLM KYKLPRP+ FSFAFL+TC+
Sbjct: 361 GQVGESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCV 420

Query: 421 GQLCIAYPSIGSVYIASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNWGQLIVPFGSY 480
           GQL IAYP  GSVY+A++IIGFGFGAQ P+LFA+ISE+FGL+ YSTLFN GQL VP GSY
Sbjct: 421 GQLFIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSY 480

Query: 481 ILNVDVVGKLYDVEALREGLNNNNIKLGKAITCMGAHCFSGSFTILASVTLFAALTMLVL 540
           ILNVDVVGKLYD+EALREG        GK +TC GAHCF GSFTILA+ TLF A+ MLVL
Sbjct: 481 ILNVDVVGKLYDMEALREGKK----IAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVL 540

Query: 541 AYRTRDFYKGDVYNKYKEDLWIPQSEMEFYRLDNRKRIEE 581
           AYRTR+FYK DVY  + E++WIPQ++MEFYRLD++K IE+
Sbjct: 541 AYRTREFYKRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED 573

[Close]

BLAST of Tan0008479 vs. NCBI nr
Match: XP_022970240.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 828.6 bits (2139), Expect = 3.4e-236
Identity = 419/580 (72.24%), Postives = 482/580 (83.10%), Query Frame = 0


Query: 1   MSNNRRSEIWGFIKQVVGGRWFSVFASFLLMIGNGTTYIYGTYSKVIKTQFDYNQTQINT 60
           M N   SE WGFIKQVV GRWFSVFA  ++MIGNGTTY++ TYSKVIKTQFDY+QTQINT
Sbjct: 1   MGNKGGSEAWGFIKQVVTGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNVGILAGLLAEVVPLWVLLLVGGFLNFISYFMIWLSITHRIAKPQFWQMFL 120
           LGFAKDLGSNVGI+AGLLAEV P WVL +VG F NF  +F+IWLSIT RIAKP+FWQMF+
Sbjct: 61  LGFAKDLGSNVGIIAGLLAEVTPTWVLFIVGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICLAANSANFSNTAILVTSVRNFPDRRGIILGLIKGFVGIAGAILTQFYLAIYGHEDPS 180
            +C   NS+N++NTAI+VTSVRNFPDRRGIILGL+KG+VGI GAI++Q YLAIYGH DPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQLYLAIYGHNDPS 180

Query: 181 NQILLFAWLPSTIILIFFKSIRPINVRKQPDELTVFYHLLYVSMLLAIFLLFLTLAQKQV 240
           N +LLFAWLPS +ILI F SIRPI +RK P+EL VFY LLYVS+LLAIF+LFLT+AQKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 TFSHVGFMSGALVIGCLLCLPLLIAFREEYFLYKHNNDIDSSSSVTVSIVDNQKPPLLAV 300
            FS  G++ GA  +  LLC+P+LIA REE  LYK N    +  SVTVS+V +Q PP L+ 
Sbjct: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQ-TAGPSVTVSMV-HQNPPTLSA 300

Query: 301 PEKPTEVVENSPSCFSNIMNKPARGEDFTIFQAMFSKDMALICLATFSGCGSSLAAIDNL 360
               +E+ E  PSC  NI NKP RGEDFTI QA+FSKDMALICLATFSGCGSSLAAIDNL
Sbjct: 301 -SADSEIPEILPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNL 360

Query: 361 GQIGESLGYPQQAISIFVSWISIFNFFGRVFSGFISETLMLKYKLPRPVIFSFAFLVTCL 420
           GQ+GESLGYPQ+AI I VSW+SIFNFFGRVFSGFISETLM KYKLPRP+ FSFAFL+TC+
Sbjct: 361 GQVGESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCV 420

Query: 421 GQLCIAYPSIGSVYIASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNWGQLIVPFGSY 480
           GQL IAYP  GSVY A++IIGFGFGAQ P+LFA+ISE+FGL+HYSTLFN GQL VP GSY
Sbjct: 421 GQLFIAYPFPGSVYFAAIIIGFGFGAQNPMLFAVISEMFGLRHYSTLFNCGQLTVPLGSY 480

Query: 481 ILNVDVVGKLYDVEALREGLNNNNIKLGKAITCMGAHCFSGSFTILASVTLFAALTMLVL 540
           ILNVDVVGKLYD+EAL EG        GK +TC GAHCF GSFTILA+ TLF A+ MLVL
Sbjct: 481 ILNVDVVGKLYDMEALHEGKQ----IAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVL 540

Query: 541 AYRTRDFYKGDVYNKYKEDLWIPQSEMEFYRLDNRKRIEE 581
           AYRTR+FYK DVY  + E++WIPQ++MEFYRLDN+K IE+
Sbjct: 541 AYRTREFYKRDVYKNFNEEIWIPQTQMEFYRLDNKKSIED 573

[Close]

BLAST of Tan0008479 vs. NCBI nr
Match: XP_022964774.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 823.9 bits (2127), Expect = 8.5e-235
Identity = 413/580 (71.21%), Postives = 481/580 (82.93%), Query Frame = 0


Query: 1   MSNNRRSEIWGFIKQVVGGRWFSVFASFLLMIGNGTTYIYGTYSKVIKTQFDYNQTQINT 60
           M N   SE W FIKQVV GRWFSVFA  ++MIGNGTTY++ TYSKVIKTQFDY+QTQINT
Sbjct: 1   MGNKGGSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNVGILAGLLAEVVPLWVLLLVGGFLNFISYFMIWLSITHRIAKPQFWQMFL 120
           LGFAKDLGSN+GI+AGLLAEV P WVL ++G F NF  +F+IWLSIT RIAKP+FWQMF+
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICLAANSANFSNTAILVTSVRNFPDRRGIILGLIKGFVGIAGAILTQFYLAIYGHEDPS 180
            +C A NS+N++NTAI+VTSVRNFPDRRGIILGL+KG+VGI GAI++Q YLAIYGH DPS
Sbjct: 121 LVCFATNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPS 180

Query: 181 NQILLFAWLPSTIILIFFKSIRPINVRKQPDELTVFYHLLYVSMLLAIFLLFLTLAQKQV 240
           N +LLFAWLPS +ILI F SIRPI +RK PDEL VFY LLYVS+LLAIF+LFLT+AQKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 TFSHVGFMSGALVIGCLLCLPLLIAFREEYFLYKHNNDIDSSSSVTVSIVDNQKPPLLAV 300
            FS  G++ GA  + CLLC+P+LIA REE  LYK N    ++ SV +S++ +Q PP L  
Sbjct: 241 VFSQAGYVGGASFVVCLLCVPILIACREELLLYKLNKQ-TAAPSVALSML-HQNPPTLPT 300

Query: 301 PEKPTEVVENSPSCFSNIMNKPARGEDFTIFQAMFSKDMALICLATFSGCGSSLAAIDNL 360
               +E+ E SPSC  NI NKP RGEDFTI QA+FSKDMALICLATFSGCGSSLAAIDNL
Sbjct: 301 -SADSEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNL 360

Query: 361 GQIGESLGYPQQAISIFVSWISIFNFFGRVFSGFISETLMLKYKLPRPVIFSFAFLVTCL 420
           GQ+GESLGY Q+AI I VSW+SIFNFFGRVFSGFISETLM KYKLPRP+ F+FAFL+TC+
Sbjct: 361 GQVGESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCV 420

Query: 421 GQLCIAYPSIGSVYIASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNWGQLIVPFGSY 480
           GQL IAYP  GS+Y+A++IIGFGFGAQ P+LFA+ISE+FGLK YSTLFN GQL  PFGSY
Sbjct: 421 GQLFIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSY 480

Query: 481 ILNVDVVGKLYDVEALREGLNNNNIKLGKAITCMGAHCFSGSFTILASVTLFAALTMLVL 540
           ILNVDVVGKLYD+EALRE         GK +TC GAHCF GSFT+LA+ TLF A+ M VL
Sbjct: 481 ILNVDVVGKLYDMEALRE----RKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFVL 540

Query: 541 AYRTRDFYKGDVYNKYKEDLWIPQSEMEFYRLDNRKRIEE 581
           AYRTR+FYK DVY  + E++WIPQ+EMEFYRLDN+K IE+
Sbjct: 541 AYRTREFYKRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

[Close]

BLAST of Tan0008479 vs. NCBI nr
Match: KAG6583526.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG6583533.1 Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG6583538.1 Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia] >KAG6583544.1 Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 823.9 bits (2127), Expect = 8.5e-235
Identity = 413/580 (71.21%), Postives = 481/580 (82.93%), Query Frame = 0


Query: 1   MSNNRRSEIWGFIKQVVGGRWFSVFASFLLMIGNGTTYIYGTYSKVIKTQFDYNQTQINT 60
           M N   SE W FIKQVV GRWFSVFA  ++MIGNGTTY++ TYSKVIKTQFDY+QTQINT
Sbjct: 1   MGNKGGSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNVGILAGLLAEVVPLWVLLLVGGFLNFISYFMIWLSITHRIAKPQFWQMFL 120
           LGFAKDLGSN+GI+AGLLAEV P WVL ++G F NF  +F+IWLSIT RIAKP+FWQMF+
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICLAANSANFSNTAILVTSVRNFPDRRGIILGLIKGFVGIAGAILTQFYLAIYGHEDPS 180
            +CL  NS+N++NTAI+VTSVRNFPDRRGIILGL+KG+VGI GAI++Q YLAIYGH DPS
Sbjct: 121 LVCLGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPS 180

Query: 181 NQILLFAWLPSTIILIFFKSIRPINVRKQPDELTVFYHLLYVSMLLAIFLLFLTLAQKQV 240
           N +LLFAWLPS +ILI F SIRPI +RK PDEL VFY LLYVS+LLAIF+LFLT+AQKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 TFSHVGFMSGALVIGCLLCLPLLIAFREEYFLYKHNNDIDSSSSVTVSIVDNQKPPLLAV 300
            FS  G++ GA  + CLLC+P+LIA REE  LYK N    ++ SV +S++ +Q PP L  
Sbjct: 241 VFSQAGYVGGASFVVCLLCVPILIACREELLLYKLNKQ-TAAPSVALSML-HQNPPTLPT 300

Query: 301 PEKPTEVVENSPSCFSNIMNKPARGEDFTIFQAMFSKDMALICLATFSGCGSSLAAIDNL 360
               +E+ E SPSC  NI NKP RGEDFTI QA+FSKDMALICLATFSGCGSSLAAIDNL
Sbjct: 301 -SADSEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNL 360

Query: 361 GQIGESLGYPQQAISIFVSWISIFNFFGRVFSGFISETLMLKYKLPRPVIFSFAFLVTCL 420
           GQ+GESLGY Q+AI I VSW+SIFNFFGRVFSGFISETLM KYKLPRP+ F+FAFL+TC+
Sbjct: 361 GQVGESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCV 420

Query: 421 GQLCIAYPSIGSVYIASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNWGQLIVPFGSY 480
           GQL IAYP  GS+Y+A++IIGFGFGAQ P+LFA+ISE+FGLK YSTLFN GQL  PFGSY
Sbjct: 421 GQLFIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSY 480

Query: 481 ILNVDVVGKLYDVEALREGLNNNNIKLGKAITCMGAHCFSGSFTILASVTLFAALTMLVL 540
           ILNVDVVGKLYD+EALRE         GK +TC GAHCF GSFT+LA+ TLF A+ M VL
Sbjct: 481 ILNVDVVGKLYDMEALRE----RKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFVL 540

Query: 541 AYRTRDFYKGDVYNKYKEDLWIPQSEMEFYRLDNRKRIEE 581
           AYRTR+FYK DVY  + E++WIPQ+EMEFYRLDN+K IE+
Sbjct: 541 AYRTREFYKRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

[Close]

BLAST of Tan0008479 vs. NCBI nr
Match: XP_022964780.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 823.2 bits (2125), Expect = 1.4e-234
Identity = 412/580 (71.03%), Postives = 481/580 (82.93%), Query Frame = 0


Query: 1   MSNNRRSEIWGFIKQVVGGRWFSVFASFLLMIGNGTTYIYGTYSKVIKTQFDYNQTQINT 60
           M N   SE W FIKQVV GRWFSVFA  ++MIGNGTTY++ TYSKVIKTQFDY+QTQINT
Sbjct: 1   MGNKGGSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNVGILAGLLAEVVPLWVLLLVGGFLNFISYFMIWLSITHRIAKPQFWQMFL 120
           LGFAKDLGSN+GI+AGLLAEV P WVL ++G F NF  +F+IWLSIT RIAKP+FWQMF+
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICLAANSANFSNTAILVTSVRNFPDRRGIILGLIKGFVGIAGAILTQFYLAIYGHEDPS 180
            +C   NS+N++NTAI+VTSVRNFPDRRGIILGL+KG+VGI GAI++Q YLAIYGH DPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPS 180

Query: 181 NQILLFAWLPSTIILIFFKSIRPINVRKQPDELTVFYHLLYVSMLLAIFLLFLTLAQKQV 240
           N +LLFAWLPS +ILI F SIRPI +RK PDEL VFY LLYVS+LLAIF+LFLT+AQKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 TFSHVGFMSGALVIGCLLCLPLLIAFREEYFLYKHNNDIDSSSSVTVSIVDNQKPPLLAV 300
            FS  G++ GA  + CLLC+P+LIA REE  LYK N +  ++ SV +S++ +Q PP L  
Sbjct: 241 VFSQAGYVGGASFVVCLLCVPILIACREELLLYKLNKE-TAAPSVALSML-HQNPPTLPT 300

Query: 301 PEKPTEVVENSPSCFSNIMNKPARGEDFTIFQAMFSKDMALICLATFSGCGSSLAAIDNL 360
               +E+ E SPSC  NI NKP RGEDFTI QA+FSKDMALICLATFSGCGSSLAAIDNL
Sbjct: 301 -SADSEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNL 360

Query: 361 GQIGESLGYPQQAISIFVSWISIFNFFGRVFSGFISETLMLKYKLPRPVIFSFAFLVTCL 420
           GQ+GESLGY Q+AI I VSW+SIFNFFGRVFSGFISETLM KYKLPRP+ F+FAFL+TC+
Sbjct: 361 GQVGESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCV 420

Query: 421 GQLCIAYPSIGSVYIASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNWGQLIVPFGSY 480
           GQL IAYP  GS+Y+A++IIGFGFGAQ P+LFA+ISE+FGLK YSTLFN GQL  PFGSY
Sbjct: 421 GQLFIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSY 480

Query: 481 ILNVDVVGKLYDVEALREGLNNNNIKLGKAITCMGAHCFSGSFTILASVTLFAALTMLVL 540
           ILNVDVVGKLYD+EALRE         GK +TC GAHCF GSFT+LA+ TLF A+ M VL
Sbjct: 481 ILNVDVVGKLYDMEALRE----RKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFVL 540

Query: 541 AYRTRDFYKGDVYNKYKEDLWIPQSEMEFYRLDNRKRIEE 581
           AYRTR+FYK DVY  + E++WIPQ+EMEFYRLDN+K IE+
Sbjct: 541 AYRTREFYKRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

[Close]

BLAST of Tan0008479 vs. ExPASy TrEMBL
Match: A0A6J1I5D7 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469398 PE=4 SV=1)

HSP 1 Score: 832.0 bits (2148), Expect = 1.5e-237
Identity = 419/580 (72.24%), Postives = 485/580 (83.62%), Query Frame = 0


Query: 1   MSNNRRSEIWGFIKQVVGGRWFSVFASFLLMIGNGTTYIYGTYSKVIKTQFDYNQTQINT 60
           M N   SE WGFIKQVV GRWFSVFA  ++MIGNGTTY++ TYSKVIKTQFDY+QTQINT
Sbjct: 1   MGNKGGSEAWGFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNVGILAGLLAEVVPLWVLLLVGGFLNFISYFMIWLSITHRIAKPQFWQMFL 120
           LGFAKDLGSN+GI+AGLLAEV P WVL ++G F NFI +F+IWLSIT RIAKP+FWQMF+
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFIGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICLAANSANFSNTAILVTSVRNFPDRRGIILGLIKGFVGIAGAILTQFYLAIYGHEDPS 180
            +C   NS+N++NTAI+VTSVRNFPDRRGIILGL+KG+VGI GAI++QFYLAIYGH DPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQFYLAIYGHNDPS 180

Query: 181 NQILLFAWLPSTIILIFFKSIRPINVRKQPDELTVFYHLLYVSMLLAIFLLFLTLAQKQV 240
           N +LLFAWLPS +ILI F SIRPI +RK P+EL VFY LLYVS+LLAIF+LFLT+AQKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 TFSHVGFMSGALVIGCLLCLPLLIAFREEYFLYKHNNDIDSSSSVTVSIVDNQKPPLLAV 300
            FS  G++ GA  +  LLC+P+LIA REE  LYK N    +  SVTVS+V +Q PP L  
Sbjct: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQ-TAGPSVTVSMV-HQNPPTLPA 300

Query: 301 PEKPTEVVENSPSCFSNIMNKPARGEDFTIFQAMFSKDMALICLATFSGCGSSLAAIDNL 360
               +E+ E SPSC  NI NKP RGEDFTI QA+FSKDMALICLATFSGCGSSLAAIDNL
Sbjct: 301 -SADSEIPEISPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNL 360

Query: 361 GQIGESLGYPQQAISIFVSWISIFNFFGRVFSGFISETLMLKYKLPRPVIFSFAFLVTCL 420
           GQ+GESLGYPQ+AI I VSW+SIFNFFGRVFSGFISETLM KYKLPRP+ FSFAFL+TC+
Sbjct: 361 GQVGESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCV 420

Query: 421 GQLCIAYPSIGSVYIASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNWGQLIVPFGSY 480
           GQL IAYP  GSVY+A++IIGFGFGAQ P+LFA+ISE+FGL+ YSTLFN GQL VP GSY
Sbjct: 421 GQLFIAYPFPGSVYLAAIIIGFGFGAQNPMLFAVISEMFGLRRYSTLFNCGQLAVPLGSY 480

Query: 481 ILNVDVVGKLYDVEALREGLNNNNIKLGKAITCMGAHCFSGSFTILASVTLFAALTMLVL 540
           ILNVDVVGKLYD+EALREG        GK +TC GAHCF GSFTILA+ TLF A+ MLVL
Sbjct: 481 ILNVDVVGKLYDMEALREGKK----IAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVL 540

Query: 541 AYRTRDFYKGDVYNKYKEDLWIPQSEMEFYRLDNRKRIEE 581
           AYRTR+FYK DVY  + E++WIPQ++MEFYRLD++K IE+
Sbjct: 541 AYRTREFYKRDVYKNFNEEIWIPQTQMEFYRLDSKKSIED 573

[Close]

BLAST of Tan0008479 vs. ExPASy TrEMBL
Match: A0A6J1I2B3 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469248 PE=4 SV=1)

HSP 1 Score: 828.6 bits (2139), Expect = 1.7e-236
Identity = 419/580 (72.24%), Postives = 482/580 (83.10%), Query Frame = 0


Query: 1   MSNNRRSEIWGFIKQVVGGRWFSVFASFLLMIGNGTTYIYGTYSKVIKTQFDYNQTQINT 60
           M N   SE WGFIKQVV GRWFSVFA  ++MIGNGTTY++ TYSKVIKTQFDY+QTQINT
Sbjct: 1   MGNKGGSEAWGFIKQVVTGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNVGILAGLLAEVVPLWVLLLVGGFLNFISYFMIWLSITHRIAKPQFWQMFL 120
           LGFAKDLGSNVGI+AGLLAEV P WVL +VG F NF  +F+IWLSIT RIAKP+FWQMF+
Sbjct: 61  LGFAKDLGSNVGIIAGLLAEVTPTWVLFIVGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICLAANSANFSNTAILVTSVRNFPDRRGIILGLIKGFVGIAGAILTQFYLAIYGHEDPS 180
            +C   NS+N++NTAI+VTSVRNFPDRRGIILGL+KG+VGI GAI++Q YLAIYGH DPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIMSQLYLAIYGHNDPS 180

Query: 181 NQILLFAWLPSTIILIFFKSIRPINVRKQPDELTVFYHLLYVSMLLAIFLLFLTLAQKQV 240
           N +LLFAWLPS +ILI F SIRPI +RK P+EL VFY LLYVS+LLAIF+LFLT+AQKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPNELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 TFSHVGFMSGALVIGCLLCLPLLIAFREEYFLYKHNNDIDSSSSVTVSIVDNQKPPLLAV 300
            FS  G++ GA  +  LLC+P+LIA REE  LYK N    +  SVTVS+V +Q PP L+ 
Sbjct: 241 VFSQAGYVGGASFVVSLLCVPILIACREELLLYKLNKQ-TAGPSVTVSMV-HQNPPTLSA 300

Query: 301 PEKPTEVVENSPSCFSNIMNKPARGEDFTIFQAMFSKDMALICLATFSGCGSSLAAIDNL 360
               +E+ E  PSC  NI NKP RGEDFTI QA+FSKDMALICLATFSGCGSSLAAIDNL
Sbjct: 301 -SADSEIPEILPSCCENIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNL 360

Query: 361 GQIGESLGYPQQAISIFVSWISIFNFFGRVFSGFISETLMLKYKLPRPVIFSFAFLVTCL 420
           GQ+GESLGYPQ+AI I VSW+SIFNFFGRVFSGFISETLM KYKLPRP+ FSFAFL+TC+
Sbjct: 361 GQVGESLGYPQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFSFAFLITCV 420

Query: 421 GQLCIAYPSIGSVYIASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNWGQLIVPFGSY 480
           GQL IAYP  GSVY A++IIGFGFGAQ P+LFA+ISE+FGL+HYSTLFN GQL VP GSY
Sbjct: 421 GQLFIAYPFPGSVYFAAIIIGFGFGAQNPMLFAVISEMFGLRHYSTLFNCGQLTVPLGSY 480

Query: 481 ILNVDVVGKLYDVEALREGLNNNNIKLGKAITCMGAHCFSGSFTILASVTLFAALTMLVL 540
           ILNVDVVGKLYD+EAL EG        GK +TC GAHCF GSFTILA+ TLF A+ MLVL
Sbjct: 481 ILNVDVVGKLYDMEALHEGKQ----IAGKGLTCTGAHCFGGSFTILAAATLFGAVVMLVL 540

Query: 541 AYRTRDFYKGDVYNKYKEDLWIPQSEMEFYRLDNRKRIEE 581
           AYRTR+FYK DVY  + E++WIPQ++MEFYRLDN+K IE+
Sbjct: 541 AYRTREFYKRDVYKNFNEEIWIPQTQMEFYRLDNKKSIED 573

[Close]

BLAST of Tan0008479 vs. ExPASy TrEMBL
Match: A0A6J1HP70 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464775 PE=4 SV=1)

HSP 1 Score: 823.9 bits (2127), Expect = 4.1e-235
Identity = 413/580 (71.21%), Postives = 481/580 (82.93%), Query Frame = 0


Query: 1   MSNNRRSEIWGFIKQVVGGRWFSVFASFLLMIGNGTTYIYGTYSKVIKTQFDYNQTQINT 60
           M N   SE W FIKQVV GRWFSVFA  ++MIGNGTTY++ TYSKVIKTQFDY+QTQINT
Sbjct: 1   MGNKGGSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNVGILAGLLAEVVPLWVLLLVGGFLNFISYFMIWLSITHRIAKPQFWQMFL 120
           LGFAKDLGSN+GI+AGLLAEV P WVL ++G F NF  +F+IWLSIT RIAKP+FWQMF+
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICLAANSANFSNTAILVTSVRNFPDRRGIILGLIKGFVGIAGAILTQFYLAIYGHEDPS 180
            +C A NS+N++NTAI+VTSVRNFPDRRGIILGL+KG+VGI GAI++Q YLAIYGH DPS
Sbjct: 121 LVCFATNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPS 180

Query: 181 NQILLFAWLPSTIILIFFKSIRPINVRKQPDELTVFYHLLYVSMLLAIFLLFLTLAQKQV 240
           N +LLFAWLPS +ILI F SIRPI +RK PDEL VFY LLYVS+LLAIF+LFLT+AQKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 TFSHVGFMSGALVIGCLLCLPLLIAFREEYFLYKHNNDIDSSSSVTVSIVDNQKPPLLAV 300
            FS  G++ GA  + CLLC+P+LIA REE  LYK N    ++ SV +S++ +Q PP L  
Sbjct: 241 VFSQAGYVGGASFVVCLLCVPILIACREELLLYKLNKQ-TAAPSVALSML-HQNPPTLPT 300

Query: 301 PEKPTEVVENSPSCFSNIMNKPARGEDFTIFQAMFSKDMALICLATFSGCGSSLAAIDNL 360
               +E+ E SPSC  NI NKP RGEDFTI QA+FSKDMALICLATFSGCGSSLAAIDNL
Sbjct: 301 -SADSEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNL 360

Query: 361 GQIGESLGYPQQAISIFVSWISIFNFFGRVFSGFISETLMLKYKLPRPVIFSFAFLVTCL 420
           GQ+GESLGY Q+AI I VSW+SIFNFFGRVFSGFISETLM KYKLPRP+ F+FAFL+TC+
Sbjct: 361 GQVGESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCV 420

Query: 421 GQLCIAYPSIGSVYIASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNWGQLIVPFGSY 480
           GQL IAYP  GS+Y+A++IIGFGFGAQ P+LFA+ISE+FGLK YSTLFN GQL  PFGSY
Sbjct: 421 GQLFIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSY 480

Query: 481 ILNVDVVGKLYDVEALREGLNNNNIKLGKAITCMGAHCFSGSFTILASVTLFAALTMLVL 540
           ILNVDVVGKLYD+EALRE         GK +TC GAHCF GSFT+LA+ TLF A+ M VL
Sbjct: 481 ILNVDVVGKLYDMEALRE----RKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFVL 540

Query: 541 AYRTRDFYKGDVYNKYKEDLWIPQSEMEFYRLDNRKRIEE 581
           AYRTR+FYK DVY  + E++WIPQ+EMEFYRLDN+K IE+
Sbjct: 541 AYRTREFYKRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

[Close]

BLAST of Tan0008479 vs. ExPASy TrEMBL
Match: A0A6J1HLT1 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464780 PE=4 SV=1)

HSP 1 Score: 823.2 bits (2125), Expect = 7.0e-235
Identity = 412/580 (71.03%), Postives = 481/580 (82.93%), Query Frame = 0


Query: 1   MSNNRRSEIWGFIKQVVGGRWFSVFASFLLMIGNGTTYIYGTYSKVIKTQFDYNQTQINT 60
           M N   SE W FIKQVV GRWFSVFA  ++MIGNGTTY++ TYSKVIKTQFDY+QTQINT
Sbjct: 1   MGNKGGSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNVGILAGLLAEVVPLWVLLLVGGFLNFISYFMIWLSITHRIAKPQFWQMFL 120
           LGFAKDLGSN+GI+AGLLAEV P WVL ++G F NF  +F+IWLSIT RIAKP+FWQMF+
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICLAANSANFSNTAILVTSVRNFPDRRGIILGLIKGFVGIAGAILTQFYLAIYGHEDPS 180
            +C   NS+N++NTAI+VTSVRNFPDRRGIILGL+KG+VGI GAI++Q YLAIYGH DPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPS 180

Query: 181 NQILLFAWLPSTIILIFFKSIRPINVRKQPDELTVFYHLLYVSMLLAIFLLFLTLAQKQV 240
           N +LLFAWLPS +ILI F SIRPI +RK PDEL VFY LLYVS+LLAIF+LFLT+AQKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 TFSHVGFMSGALVIGCLLCLPLLIAFREEYFLYKHNNDIDSSSSVTVSIVDNQKPPLLAV 300
            FS  G++ GA  + CLLC+P+LIA REE  LYK N +  ++ SV +S++ +Q PP L  
Sbjct: 241 VFSQAGYVGGASFVVCLLCVPILIACREELLLYKLNKE-TAAPSVALSML-HQNPPTLPT 300

Query: 301 PEKPTEVVENSPSCFSNIMNKPARGEDFTIFQAMFSKDMALICLATFSGCGSSLAAIDNL 360
               +E+ E SPSC  NI NKP RGEDFTI QA+FSKDMALICLATFSGCGSSLAAIDNL
Sbjct: 301 -SADSEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNL 360

Query: 361 GQIGESLGYPQQAISIFVSWISIFNFFGRVFSGFISETLMLKYKLPRPVIFSFAFLVTCL 420
           GQ+GESLGY Q+AI I VSW+SIFNFFGRVFSGFISETLM KYKLPRP+ F+FAFL+TC+
Sbjct: 361 GQVGESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCV 420

Query: 421 GQLCIAYPSIGSVYIASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNWGQLIVPFGSY 480
           GQL IAYP  GS+Y+A++IIGFGFGAQ P+LFA+ISE+FGLK YSTLFN GQL  PFGSY
Sbjct: 421 GQLFIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSY 480

Query: 481 ILNVDVVGKLYDVEALREGLNNNNIKLGKAITCMGAHCFSGSFTILASVTLFAALTMLVL 540
           ILNVDVVGKLYD+EALRE         GK +TC GAHCF GSFT+LA+ TLF A+ M VL
Sbjct: 481 ILNVDVVGKLYDMEALRE----RKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFVL 540

Query: 541 AYRTRDFYKGDVYNKYKEDLWIPQSEMEFYRLDNRKRIEE 581
           AYRTR+FYK DVY  + E++WIPQ+EMEFYRLDN+K IE+
Sbjct: 541 AYRTREFYKRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

[Close]

BLAST of Tan0008479 vs. ExPASy TrEMBL
Match: A0A6J1HLS1 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111464770 PE=4 SV=1)

HSP 1 Score: 821.6 bits (2121), Expect = 2.0e-234
Identity = 411/580 (70.86%), Postives = 481/580 (82.93%), Query Frame = 0


Query: 1   MSNNRRSEIWGFIKQVVGGRWFSVFASFLLMIGNGTTYIYGTYSKVIKTQFDYNQTQINT 60
           M N   SE W FIKQVV GRWFSVFA  ++MIGNGTTY++ TYSKVIKTQFDY+QTQINT
Sbjct: 1   MGNKGGSEAWAFIKQVVAGRWFSVFAGLIMMIGNGTTYVFPTYSKVIKTQFDYSQTQINT 60

Query: 61  LGFAKDLGSNVGILAGLLAEVVPLWVLLLVGGFLNFISYFMIWLSITHRIAKPQFWQMFL 120
           LGFAKDLGSN+GI+AGLLAEV P WVL ++G F NF  +F+IWLSIT RIAKP+FWQMF+
Sbjct: 61  LGFAKDLGSNIGIIAGLLAEVTPTWVLFIIGAFQNFTGFFLIWLSITARIAKPKFWQMFI 120

Query: 121 YICLAANSANFSNTAILVTSVRNFPDRRGIILGLIKGFVGIAGAILTQFYLAIYGHEDPS 180
            +C   NS+N++NTAI+VTSVRNFPDRRGIILGL+KG+VGI GAI++Q YLAIYGH DPS
Sbjct: 121 LVCFGTNSSNWANTAIMVTSVRNFPDRRGIILGLLKGYVGIGGAIISQLYLAIYGHNDPS 180

Query: 181 NQILLFAWLPSTIILIFFKSIRPINVRKQPDELTVFYHLLYVSMLLAIFLLFLTLAQKQV 240
           N +LLFAWLPS +ILI F SIRPI +RK PDEL VFY LLYVS+LLAIF+LFLT+AQKQV
Sbjct: 181 NLVLLFAWLPSLLILILFPSIRPIRIRKHPDELKVFYQLLYVSILLAIFILFLTIAQKQV 240

Query: 241 TFSHVGFMSGALVIGCLLCLPLLIAFREEYFLYKHNNDIDSSSSVTVSIVDNQKPPLLAV 300
            FS  G++ GA  + CLLC+P+LIA REE  LYK N +  ++ SV +S++ +Q PP L  
Sbjct: 241 VFSQAGYVGGASFVVCLLCVPILIACREELLLYKLNKE-TAAPSVALSML-HQNPPTLPT 300

Query: 301 PEKPTEVVENSPSCFSNIMNKPARGEDFTIFQAMFSKDMALICLATFSGCGSSLAAIDNL 360
               +E+ E SPSC  NI NKP RGEDFTI QA+FSKDMALICLATFSGCGSSLAAIDNL
Sbjct: 301 -SADSEIPEISPSCCPNIWNKPERGEDFTILQAVFSKDMALICLATFSGCGSSLAAIDNL 360

Query: 361 GQIGESLGYPQQAISIFVSWISIFNFFGRVFSGFISETLMLKYKLPRPVIFSFAFLVTCL 420
           GQ+GESLGY Q+AI I VSW+SIFNFFGRVFSGFISETLM KYKLPRP+ F+FAFL+TC+
Sbjct: 361 GQVGESLGYSQRAIGILVSWVSIFNFFGRVFSGFISETLMTKYKLPRPLTFAFAFLITCV 420

Query: 421 GQLCIAYPSIGSVYIASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNWGQLIVPFGSY 480
           GQL IAYP  GS+Y+A++IIGFGFGAQ P+LFA+ISE+FGLK YSTLFN GQL  PFGSY
Sbjct: 421 GQLFIAYPFPGSLYLAAIIIGFGFGAQNPMLFAVISEMFGLKRYSTLFNCGQLAAPFGSY 480

Query: 481 ILNVDVVGKLYDVEALREGLNNNNIKLGKAITCMGAHCFSGSFTILASVTLFAALTMLVL 540
           ILNVDVVGKLYD+EALRE         GK +TC GAHCF GSFT+LA+ TLF A+ M VL
Sbjct: 481 ILNVDVVGKLYDMEALRE----RKQIAGKGLTCTGAHCFGGSFTVLAAATLFGAVVMFVL 540

Query: 541 AYRTRDFYKGDVYNKYKEDLWIPQSEMEFYRLDNRKRIEE 581
           AY+TR+FYK DVY  + E++WIPQ+EMEFYRLDN+K IE+
Sbjct: 541 AYQTREFYKRDVYKNFNEEIWIPQTEMEFYRLDNKKNIED 573

[Close]

BLAST of Tan0008479 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 574.3 bits (1479), Expect = 1.1e-163
Identity = 305/576 (52.95%), Postives = 406/576 (70.49%), Query Frame = 0


Query: 12  FIKQVVGGRWFSVFASFLLMIGNGTTYIYGTYSKVIKTQFDYNQTQINTLGFAKDLGSNV 71
           F+     GRWF VFASFL+M   G TY++GTYSK IK+   Y+QT +N LGF KDLG+NV
Sbjct: 9   FLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANV 68

Query: 72  GILAGLLAEVVPLWVLLLVGGFLNFISYFMIWLSITHRIAKPQFWQMFLYICLAANSANF 131
           G+L+GL+AEV P W +L +G  +NF+ YFMIWL++T ++AKP+ WQM LYIC+ ANS NF
Sbjct: 69  GVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNF 128

Query: 132 SNTAILVTSVRNFPDRRGIILGLIKGFVGIAGAILTQFYLAIYGHEDPSNQILLFAWLPS 191
           +NT  LVT V+NFP+ RG++LGL+KG+VG++GAI TQ Y AIYGH D  + ILL AWLP+
Sbjct: 129 ANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGH-DSKSLILLIAWLPA 188

Query: 192 TIILIFFKSIRPINVRKQPDELTVFYHLLYVSMLLAIFLLFLTLAQKQVTFSHVGFMSGA 251
            + L+F   IR   V +Q +EL+VFY  LY+S+ LA+FL+ + +A+KQV FS   + + A
Sbjct: 189 AVSLVFVYLIREKKVVRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAASA 248

Query: 252 LVIGCLLCLPLLIAFREEYFLYKHNN-DIDSSSSVTVSIVDNQKPPLLAVPEKPTEV--- 311
            +   LL +PL ++ ++E  ++      I+  S V    V+  K  L    +K  +V   
Sbjct: 249 TICCALLFVPLTVSVKQELEVWNMMKLPIEEPSEVK---VEKPKKELDLDQDKAAKVNGE 308

Query: 312 VENSPSCFSNIMNKPARGEDFTIFQAMFSKDMALICLATFSGCGSSLAAIDNLGQIGESL 371
            + + SCFS + + P RGED+TI QA+ S DM ++ +ATF G GSSL A+DNLGQIGESL
Sbjct: 309 EKETKSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQIGESL 368

Query: 372 GYPQQAISIFVSWISIFNFFGRVFSGFISETLMLKYKLPRPVIFSFAFLVTCLGQLCIAY 431
           GYP   +S FVS +SI+N+FGRVFSGF+SE L+ KYKLPRP++ +   L++C G L IA+
Sbjct: 369 GYPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGHLLIAF 428

Query: 432 PSIGSVYIASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNWGQLIVPFGSYILNVDVV 491
           P  GSVYIAS+++GF FGAQ+PLLFAIISELFGLK+YSTLFN GQL  P GSYILNV V 
Sbjct: 429 PVPGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYILNVRVT 488

Query: 492 GKLYDVEALRE----GLNNNNIKLGKAITCMGAHCFSGSFTILASVTLFAALTMLVLAYR 551
           G LYD EAL++    GL   ++   K +TC+G+ C+   F ILA+VT F AL  L LA R
Sbjct: 489 GMLYDKEALKQLTARGLTRKDV---KDLTCLGSQCYKLPFLILAAVTFFGALVSLGLAIR 548

Query: 552 TRDFYKGDVYNKYKEDLWIPQSEMEFYRLDNRKRIE 580
           TR+FYKGD+Y K++E    P+SE E    D+RK +E
Sbjct: 549 TREFYKGDIYKKFRES---PESESELVP-DSRKAVE 573

[Close]

BLAST of Tan0008479 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 523.5 bits (1347), Expect = 2.2e-148
Identity = 275/563 (48.85%), Postives = 382/563 (67.85%), Query Frame = 0


Query: 15  QVVGGRWFSVFASFLLMIGNGTTYIYGTYSKVIKTQFDYNQTQINTLGFAKDLGSNVGIL 74
           Q++ GRWF  F S L+M   G TY++G YS  IK    Y+QT +N L F KDLG+NVG+L
Sbjct: 16  QILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLGANVGVL 75

Query: 75  AGLLAEVVPLWVLLLVGGFLNFISYFMIWLSITHRIAKPQFWQMFLYICLAANSANFSNT 134
           AGLL EV P W +LL+G  LNF  YFMIWL++T RI+KPQ W M LYIC+ ANS +F+NT
Sbjct: 76  AGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANSQSFANT 135

Query: 135 AILVTSVRNFPDRRGIILGLIKGFVGIAGAILTQFYLAIYGHEDPSNQILLFAWLPSTII 194
             LVT V+NFP+ RG++LG++KG+VG++GAI+TQ Y A YG ED    IL+  WLP+ + 
Sbjct: 136 GSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYG-EDTKELILMIGWLPAIVS 195

Query: 195 LIFFKSIRPINVRKQPDELTVFYHLLYVSMLLAIFLLFLTLAQKQVTFSHVGFMSGALVI 254
             F ++IR + V++Q +EL VFY+ LY+S+ LA FL+ + +  K   F+   F   A V+
Sbjct: 196 FAFLRTIRIMKVKRQTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEFGGSAAVV 255

Query: 255 GCLLCLPLLIAFREEYFLYKHNNDIDSSSSVTVSIVDNQKPPLLAVP------EKPTEVV 314
             LL LP+++   EE  L+K    +  +    +++V  +KP L +        E+  EVV
Sbjct: 256 IVLLLLPIIVVILEEKKLWK-EKQVALNDPAPINVV-TEKPKLDSSEFKDDDGEESKEVV 315

Query: 315 E--NSPSCFSNIMNKPARGEDFTIFQAMFSKDMALICLATFSGCGSSLAAIDNLGQIGES 374
           E   +PSC++ + N P RG+D+TI QA+FS DM ++ LAT  G G +L AIDNLGQIG S
Sbjct: 316 EKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTLTAIDNLGQIGNS 375

Query: 375 LGYPQQAISIFVSWISIFNFFGRVFSGFISETLMLKYKLPRPVIFSFAFLVTCLGQLCIA 434
           LGYP++++S FVS +SI+N++GRV SG +SE  ++KYK PRP++ +   L++C G L IA
Sbjct: 376 LGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMVLLLSCAGHLLIA 435

Query: 435 YPSIGSVYIASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNWGQLIVPFGSYILNVDV 494
           +   G +Y+AS+IIGF FGAQ PLLFAIISE+FGLK+YSTL+N+G +  P GSY+LNV V
Sbjct: 436 FNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVASPIGSYLLNVRV 495

Query: 495 VGKLYDVEALRE--GLNNNNIKLGKAITCMGAHCFSGSFTILASVTLFAALTMLVLAYRT 554
            G LYDVEA ++   L    ++ G+ + C+G  CF  SF I+A+VTLF  L  +VL  RT
Sbjct: 496 AGYLYDVEAGKQYKALGKTRVE-GQDLNCIGTSCFKLSFIIIAAVTLFGVLVSMVLVIRT 555

Query: 555 RDFYKGDVYNKYKEDLWIPQSEM 568
           + FYK D+Y K++E     + EM
Sbjct: 556 KKFYKSDIYKKFREKALAAEMEM 574

[Close]

BLAST of Tan0008479 vs. TAIR 10
Match: AT5G14120.1 (Major facilitator superfamily protein )

HSP 1 Score: 294.7 bits (753), Expect = 1.7e-79
Identity = 192/578 (33.22%), Postives = 298/578 (51.56%), Query Frame = 0


Query: 1   MSNNRRSEIWGFIKQVVGGRWFSVFASFLLMIGNGTTYIYGTYSKVIKTQFDYNQTQINT 60
           M++  R +   FI      RW    A+  +    G  Y++G+ S VIK+  +YNQ +++ 
Sbjct: 1   MASTTREKFVSFI----NNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKELSR 60

Query: 61  LGFAKDLGSNVGILAGLLAEVVPLWVLLLVGGFLNFISYFMIWLSITHRIAKPQFWQMFL 120
           LG AKDLG +VG +AG L+E++PLW  LLVG   N I Y  +WL +T R      W M +
Sbjct: 61  LGVAKDLGDSVGFIAGTLSEILPLWAALLVGAVQNLIGYGWVWLIVTGRAPILPLWAMCV 120

Query: 121 YICLAANSANFSNTAILVTSVRNFPDRRGIILGLIKGFVGIAGAILTQFYLAIYGHEDPS 180
            I +  N   + NT  LV+ V+NFP  RG ++G++KGF G+ GAI++Q Y  I+   +P+
Sbjct: 121 LIFVGNNGETYFNTGALVSGVQNFPKSRGPVVGILKGFAGLGGAIISQIYTMIHS-SNPA 180

Query: 181 NQILLFAWLPSTIILIFFKSIRPINVRKQ--PDELTVFYHLLYVSMLLAIFLLFLTLAQK 240
           + IL+ A  P+ +++     IRP+   KQ  P +   F  +  V +LLA +L+ + L Q 
Sbjct: 181 SLILMVAVTPAVVVVCLMFFIRPVGGHKQIRPTDGASFTFIYGVCLLLAAYLMSVMLIQD 240

Query: 241 QVTFSHVGFMSGALVIGCLLCLPLLIAFREEYFLYKHNNDID----------------SS 300
            V  SH       +V+  +L +P+L+     +F   +  D                   +
Sbjct: 241 LVVVSHNVITVFTIVLFVILVVPILVPIMTSFFTETNEPDDTIEEPLVPKREDQEPGLQT 300

Query: 301 SSVTVSIVDNQKP---PLLAVPEKPTEVVENSPSCFS---------NIMNKPARGEDFTI 360
             + +S V+++KP    LL   E+   +                  N    P RGEDFT+
Sbjct: 301 PDLILSEVEDEKPKDVDLLPASERHKRIAHLQAQLMQAAAEGAVRVNRRRGPHRGEDFTL 360

Query: 361 FQAMFSKDMALICLATFSGCGSSLAAIDNLGQIGESLGYPQQAISIFVSWISIFNFFGRV 420
            QA+   D  LI  +   G GS L  IDNLGQ+ +SLGY      + VS ISI+NF GR+
Sbjct: 361 TQALVKADFWLIFFSLLLGSGSGLTVIDNLGQMSQSLGYDN--THVLVSMISIWNFLGRI 420

Query: 421 FSGFISETLMLKYKLPRPVIFSFAFLVTCLGQLCIAYPSIGSVYIASLIIGFGFGAQVPL 480
             G+ SE ++  Y  PRPV  + A L+  +G +  AY   G++YI +L+IG G+GA   +
Sbjct: 421 GGGYFSELVVRDYAYPRPVAMAVAQLIMSVGHIFFAYGWPGAMYIGTLLIGLGYGAHWAI 480

Query: 481 LFAIISELFGLKHYSTLFNWGQLIVPFGSYILNVDVVGKLYDVEALREGLNNNNIKLGKA 540
           + A  SELFGLK +  L+N+  L  P GS + +  +   +YD EA R+  + +      A
Sbjct: 481 VPATASELFGLKKFGALYNFLTLANPAGSLVFSGMIASSIYDREAERQA-HGSVFDPDDA 540

Query: 541 ITCMGAHCFSGSFTILASVTLFAALTMLVLAYRTRDFY 549
           + C G+ CF  +  I++   + A +  ++L  RT+  Y
Sbjct: 541 LRCNGSICFFLTSLIMSGFCIIACMLSMILVRRTKSVY 570

[Close]

BLAST of Tan0008479 vs. TAIR 10
Match: AT3G01930.2 (Major facilitator superfamily protein )

HSP 1 Score: 290.0 bits (741), Expect = 4.1e-78
Identity = 194/578 (33.56%), Postives = 295/578 (51.04%), Query Frame = 0


Query: 13  IKQVVGGRWFSVFASFLLMIGNGTTYIYGTYSKVIKTQFDYNQTQINTLGFAKDLGSNVG 72
           +K  +  RW    A+  +    G  Y++G+ S VIK+  +YNQ Q++ LG AKDLG +VG
Sbjct: 9   VKSFINNRWLVFVAAMWIQSCAGIGYLFGSISPVIKSSLNYNQKQLSRLGVAKDLGDSVG 68

Query: 73  ILAGLLAEVVPLWVLLLVGGFLNFISYFMIWLSITHRIAKPQFWQMFLYICLAANSANFS 132
            LAG L+E++PLW  LLVG   N + Y  +WL +T R      W M + I +  N   + 
Sbjct: 69  FLAGTLSEILPLWAALLVGSVQNLVGYGWVWLIVTGRAPILPLWAMCILIFVGNNGETYF 128

Query: 133 NTAILVTSVRNFPDRRGIILGLIKGFVGIAGAILTQFYLAIYGHEDPSNQILLFAWLPST 192
           NTA LV+ V+NFP  RG ++G++KGF G+ GAIL+Q Y  I+   D ++ I + A  PS 
Sbjct: 129 NTAALVSGVQNFPKSRGPVVGILKGFAGLGGAILSQVYTMIHS-SDRASLIFMVAVAPSV 188

Query: 193 IILIFFKSIRPINVRKQ--PDELTVFYHLLYVSMLLAIFLLFLTLAQKQVTFSHVGFMSG 252
           +++     IRP+   +Q    + T F  +  V +LLA +L+ + L +  +  SH   ++ 
Sbjct: 189 VVVPLMFFIRPVGGHRQIRSSDATSFTVIYAVCILLAAYLMAVMLVEDFIDLSHSIIIAF 248

Query: 253 ALVIGCLLCLPLLIAFREEYF-------------LYKHNNDIDSSSSVT--------VSI 312
            +V+  +L +P+ I      F             L       D   S T         S 
Sbjct: 249 TVVLFAILLVPIFIPIATSCFTASTDPCDTLEEPLLGDQQGQDPGQSTTPDHGPELIFSE 308

Query: 313 VDNQKP---PLLAVPEKPTEVVENSPSCFS---------NIMNKPARGEDFTIFQAMFSK 372
           V+++KP    LL   E+   + +                     P RGEDFT+ QA+   
Sbjct: 309 VEDEKPKEVDLLPAVERHKRIAQLQAKLMQAAAEGAVRVKRRRGPHRGEDFTLTQALVKA 368

Query: 373 DMALICLATFSGCGSSLAAIDNLGQIGESLGYPQQAISIFVSWISIFNFFGRVFSGFISE 432
           D  LI  +   G GS L  IDNLGQ+ +SLGY      +FVS ISI+NF GR+  G+ SE
Sbjct: 369 DFWLIFFSLLLGSGSGLTVIDNLGQMSQSLGYDN--THVFVSMISIWNFLGRIGGGYFSE 428

Query: 433 TLMLKYKLPRPVIFSFAFLVTCLGQLCIAYPSIGSVYIASLIIGFGFGAQVPLLFAIISE 492
            ++  Y  PRPV  + A LV  +G +  AY   G+++I +L+IG G+GA   ++ A  SE
Sbjct: 429 LIVRDYAYPRPVAIAVAQLVMSVGHIFFAYGWPGAMHIGTLLIGLGYGAHWAIVPATASE 488

Query: 493 LFGLKHYSTLFNWGQLIVPFGSYILNVDVVGKLYDVEALREGLNNNNIKLGKAITCMGAH 552
           LFGLK +  L+N+  L  P GS + +  +   +YD EA R+    +       + C G+ 
Sbjct: 489 LFGLKKFGALYNFLTLANPAGSLVFSGLIASSIYDREAERQA-QGSLFNPDDVLRCRGSI 548

Query: 553 CFSGSFTILASVTLFAALTMLVLAYRTRDFYKGDVYNK 556
           C+  +  I++   L AA   ++L  RT+  Y  ++Y K
Sbjct: 549 CYFLTSLIMSGFCLIAAALSMILVQRTKPVYT-NLYGK 581

[Close]

BLAST of Tan0008479 vs. TAIR 10
Match: AT5G50630.1 (Major facilitator superfamily protein )

HSP 1 Score: 270.4 bits (690), Expect = 3.4e-72
Identity = 173/541 (31.98%), Postives = 284/541 (52.50%), Query Frame = 0


Query: 13  IKQVVGGRWFSVFASFLLMIGNGTTYIY-GTYSKVIKTQFDYNQTQINTLGFAKDLGSNV 72
           ++ +V  RW     +  +    G  Y++ G+ S  IKT   YNQ QI  LG AK+LG  +
Sbjct: 9   LELLVNDRWLVFVCAMWVQSVAGVGYLFGGSMSPAIKTSLGYNQKQIALLGVAKNLGDAI 68

Query: 73  GILAGLLAEVVPLWVLLLVGGFLNFISYFMIWLSITHRIAKPQFWQMFLYICLAANSANF 132
           G ++G L+EV P WV+LLVG   N   Y ++WL +T ++     W +F+ I +  N   +
Sbjct: 69  GFVSGALSEVSPSWVVLLVGATQNLFGYGVVWLVVTGQLPNLPLWMLFVAIFVGTNGETY 128

Query: 133 SNTAILVTSVRNFPDRRGIILGLIKGFVGIAGAILTQFYLAIYGHEDPSNQILLFAWLPS 192
            NTA LV+ + NFP+ RG ++G++KGF G++GAILTQ YL ++     S+ IL+ A  P 
Sbjct: 129 YNTASLVSCIHNFPESRGPVVGILKGFSGLSGAILTQVYL-MFNPSHDSSVILMVALGPP 188

Query: 193 TIILIFFKSIRPI----NVRKQPDELTVFYHLLYVSMLLAIFLLFLTLAQKQVTFSHVGF 252
            ++L     +RP+        + D+L  F  +    ++LA++LL L + Q     +    
Sbjct: 189 VVVLALLFIVRPVERSCRTNLRSDDLR-FLAIYGFCVVLAVYLLGLLVLQSVFDMTQTII 248

Query: 253 MSGALVIGCLLCLPLLIAFREEYFLYKHNNDIDSSSSVTVSIVDNQKPPLLAVPEKPTEV 312
            +   ++   + +P+L+ F    F+  +N         T ++  ++   L+   ++P E 
Sbjct: 249 TTSGAILVIFMVVPVLVPF-SSVFISGNNVTSVKPEEGTSNVDQHEARTLIERSDRPPEK 308

Query: 313 VENSPSCFSNIMNKPARGEDFTIFQAMFSKDMALICLATFSGCGSSLAAIDNLGQIGESL 372
                         P  GEDFT+ QA+   D  LI ++   G GS +  IDNLGQI  SL
Sbjct: 309 -----------KRAPCIGEDFTLLQALGQADFWLIFMSLVLGVGSGITIIDNLGQICYSL 368

Query: 373 GYPQQAISIFVSWISIFNFFGRVFSGFISETLMLKYKLPRPVIFSFAFLVTCLGQLCIAY 432
           GY      IFVS ISI NF GRV  G+ SE ++ K  LPR +  S    +  LG +  A 
Sbjct: 369 GYSN--TKIFVSLISISNFLGRVAGGYFSELIIRKLSLPRTLAMSVVQAIMSLGLIYYAI 428

Query: 433 PSIGSVYIASLIIGFGFGAQVPLLFAIISELFGLKHYSTLFNWGQLIVPFGSYILNVDVV 492
              G +Y+ +++IG G+GA   +  A +S++FGLK + +L+N+    +P GS++ +  + 
Sbjct: 429 DWPGKIYVVTIVIGMGYGAHWAIAPASVSDIFGLKSFGSLYNFQITALPIGSFVFSGVIA 488

Query: 493 GKLYDVEALREGLNNNNIKLGKAITCMGAHCFSGSFTILASVTLFAALTMLVLAYRTRDF 549
             +YD  A ++          +++ C G+ C+S + ++++ + L A +  L + YRTR F
Sbjct: 489 SNIYDYYARKQAGPTTET---ESLVCTGSVCYSVTCSLMSMLCLMAMVLSLSVVYRTRKF 530

The following BLAST results are available for this feature:

BLAST of Tan0008479 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4I9E1	8.5e-28	25.05	Protein NUCLEAR FUSION DEFECTIVE 4 OS=Arabidopsis thaliana OX=3702 GN=NFD4 PE=3 ...	[more]
Q03795	1.7e-04	22.30	Uncharacterized membrane protein YMR155W OS=Saccharomyces cerevisiae (strain ATC...	[more]

BLAST of Tan0008479 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022970365.1	3.1e-237	72.24	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima] >XP_022970399.1 prote...	[more]
XP_022970240.1	3.4e-236	72.24	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima]	[more]
XP_022964774.1	8.5e-235	71.21	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata]	[more]
KAG6583526.1	8.5e-235	71.21	Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. soror...	[more]
XP_022964780.1	1.4e-234	71.03	protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata]	[more]

BLAST of Tan0008479 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I5D7	1.5e-237	72.24	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469...	[more]
A0A6J1I2B3	1.7e-236	72.24	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111469...	[more]
A0A6J1HP70	4.1e-235	71.21	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1HLT1	7.0e-235	71.03	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1HLS1	2.0e-234	70.86	protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]

BLAST of Tan0008479 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G28120.1	1.1e-163	52.95	Major facilitator superfamily protein	[more]
AT2G39210.1	2.2e-148	48.85	Major facilitator superfamily protein	[more]
AT5G14120.1	1.7e-79	33.22	Major facilitator superfamily protein	[more]
AT3G01930.2	4.1e-78	33.56	Major facilitator superfamily protein	[more]
AT5G50630.1	3.4e-72	31.98	Major facilitator superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036259	MFS transporter superfamily	GENE3D	1.20.1250.20	MFS general substrate transporter like domains	coord: 338..548 e-value: 6.5E-10 score: 40.7
IPR036259	MFS transporter superfamily	SUPERFAMILY	103473	MFS general substrate transporter	coord: 21..548
IPR011701	Major facilitator superfamily	PFAM	PF07690	MFS_1	coord: 343..545 e-value: 1.4E-8 score: 34.0
IPR010658	Nodulin-like	PFAM	PF06813	Nodulin-like	coord: 20..265 e-value: 1.4E-76 score: 257.3
None	No IPR available	PANTHER	PTHR21576:SF29	PROTEIN NUCLEAR FUSION DEFECTIVE 4	coord: 4..559
None	No IPR available	PANTHER	PTHR21576	UNCHARACTERIZED NODULIN-LIKE PROTEIN	coord: 4..559

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0008479.1	Tan0008479.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0022857	transmembrane transporter activity