Tan0008309 (gene) Snake gourd v1

Overview


Name	Tan0008309
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	pectinesterase inhibitor-like
Location	LG06: 79828973 .. 79829536 (+)
RNA-Seq Expression	Tan0008309
Synteny	Tan0008309

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCAATTTCAATTTCAATCTTGGAGTCTTTAGCTCTATTCAAGCTTTCCTTTGTTGTCTTCTTTTCTTCAATGTCGTTCTTTTACATGCAGATACAGAACCATCAAACGACGTCGCATCAATCGTTTGCCCGGAAACCAGAAACAGGTCTTTATGTTGGAAAGTGTTGAAGCCCGCGGGCAGCACAGATGCCCTAATGGTGGCCAATTACACCCTCTCCCTCGCCCATACGAGCGCCGTCGAAAATCTCCGCCTTGCACGACGGCTTGAGGCGGCGGAGACGGCGAATCCATTGCTGGAAAAACGCTATTCGACGTGTTCACGTCGGTTAGATTTTGCGGTGATGGGCATAGAACAAGGCAAAGAAGCGTTGGCTACTGGTGGCTATATTCTCCTCAAAGTTGCCATCAGCGCCGCCGTGGTCGACGCCAACGAATGTCTAGATGCCTTCAAACAGCCGCCGCGGGAACTGTCGTCGTTGCCGGAGAAAGCCAAGAATCTGGGAGACATCTGTGACATTGCTGTTTCTGTGTCTACTATTCTCGCCGGGGACGATTACTAA

mRNA sequence

ATGGGCAATTTCAATTTCAATCTTGGAGTCTTTAGCTCTATTCAAGCTTTCCTTTGTTGTCTTCTTTTCTTCAATGTCGTTCTTTTACATGCAGATACAGAACCATCAAACGACGTCGCATCAATCGTTTGCCCGGAAACCAGAAACAGGTCTTTATGTTGGAAAGTGTTGAAGCCCGCGGGCAGCACAGATGCCCTAATGGTGGCCAATTACACCCTCTCCCTCGCCCATACGAGCGCCGTCGAAAATCTCCGCCTTGCACGACGGCTTGAGGCGGCGGAGACGGCGAATCCATTGCTGGAAAAACGCTATTCGACGTGTTCACGTCGGTTAGATTTTGCGGTGATGGGCATAGAACAAGGCAAAGAAGCGTTGGCTACTGGTGGCTATATTCTCCTCAAAGTTGCCATCAGCGCCGCCGTGGTCGACGCCAACGAATGTCTAGATGCCTTCAAACAGCCGCCGCGGGAACTGTCGTCGTTGCCGGAGAAAGCCAAGAATCTGGGAGACATCTGTGACATTGCTGTTTCTGTGTCTACTATTCTCGCCGGGGACGATTACTAA

Coding sequence (CDS)

ATGGGCAATTTCAATTTCAATCTTGGAGTCTTTAGCTCTATTCAAGCTTTCCTTTGTTGTCTTCTTTTCTTCAATGTCGTTCTTTTACATGCAGATACAGAACCATCAAACGACGTCGCATCAATCGTTTGCCCGGAAACCAGAAACAGGTCTTTATGTTGGAAAGTGTTGAAGCCCGCGGGCAGCACAGATGCCCTAATGGTGGCCAATTACACCCTCTCCCTCGCCCATACGAGCGCCGTCGAAAATCTCCGCCTTGCACGACGGCTTGAGGCGGCGGAGACGGCGAATCCATTGCTGGAAAAACGCTATTCGACGTGTTCACGTCGGTTAGATTTTGCGGTGATGGGCATAGAACAAGGCAAAGAAGCGTTGGCTACTGGTGGCTATATTCTCCTCAAAGTTGCCATCAGCGCCGCCGTGGTCGACGCCAACGAATGTCTAGATGCCTTCAAACAGCCGCCGCGGGAACTGTCGTCGTTGCCGGAGAAAGCCAAGAATCTGGGAGACATCTGTGACATTGCTGTTTCTGTGTCTACTATTCTCGCCGGGGACGATTACTAA

Protein sequence

MGNFNFNLGVFSSIQAFLCCLLFFNVVLLHADTEPSNDVASIVCPETRNRSLCWKVLKPAGSTDALMVANYTLSLAHTSAVENLRLARRLEAAETANPLLEKRYSTCSRRLDFAVMGIEQGKEALATGGYILLKVAISAAVVDANECLDAFKQPPRELSSLPEKAKNLGDICDIAVSVSTILAGDDY

Homology

[Close]

BLAST of Tan0008309 vs. ExPASy Swiss-Prot
Match: P83326 (Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2)

HSP 1 Score: 86.3 bits (212), Expect = 4.1e-16
Identity = 52/172 (30.23%), Postives = 87/172 (50.58%), Query Frame = 0


Query: 16  AFLCCLLFFNVVLLHADTEPSNDVASIVCPETRNRSLCWKVLKP---AGSTDALMVANYT 75
           + L  +LF + +      +  N + S +CP+TRN SLC + L+    + S D   +  ++
Sbjct: 13  SLLLVILFISPLSQRPSVKAENHLISEICPKTRNPSLCLQALESDPRSASKDLKGLGQFS 72

Query: 76  LSLAHTSAVENLRLARRLEAAETANPLLEKRYSTCSRRLDFAVMGIEQGKEALATGGYIL 135
           + +A  SA +  ++   L   +  +P L+ RY TCS     A+  + Q K+ L +G Y  
Sbjct: 73  IDIAQASAKQTSKIIASL-TNQATDPKLKGRYETCSENYADAIDSLGQAKQFLTSGDYNS 132

Query: 136 LKVAISAAVVDANECLDAFKQPPRELSSLPEKAKNLGDICDIAVSVSTILAG 185
           L +  SAA   A  C D+F+ PP   + L +    L D+CDI + +S +L G
Sbjct: 133 LNIYASAAFDGAGTCEDSFEGPPNIPTQLHQADLKLEDLCDIVLVISNLLPG 183

[Close]

BLAST of Tan0008309 vs. ExPASy Swiss-Prot
Match: Q9LUV1 (Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1)

HSP 1 Score: 62.4 bits (150), Expect = 6.3e-09
Identity = 43/146 (29.45%), Postives = 70/146 (47.95%), Query Frame = 0


Query: 43  VCPETRNRSLCWKVLKPAGST---DALMVANYTLSLAHTSAVENLRLARRLEAAETANPL 102
           +C + +N+S C   +K    T   D   +AN T   A TSA E  R  + L    T NP 
Sbjct: 33  ICGKAKNQSFCTSYMKSNPKTSGADLQTLANITFGSAQTSASEGFRKIQSLVKTAT-NPT 92

Query: 103 LEKRYSTCSRRLDFAVMGIEQGKEALATGGYILLKVAISAAVVDANECLDAFKQPPRELS 162
           ++K Y++C +    A+  +   K++LA+G    L + +SAA+   + C    +Q   +  
Sbjct: 93  MKKAYTSCVQHYKSAISSLNDAKQSLASGDGKGLNIKVSAAMEGPSTC----EQDMADFK 152

Query: 163 SLPEKAKNLGD---ICDIAVSVSTIL 183
             P   KN GD   IC I + +S ++
Sbjct: 153 VDPSAVKNSGDFQNICGIVLVISNMM 173

[Close]

BLAST of Tan0008309 vs. ExPASy Swiss-Prot
Match: Q9LNF2 (Pectinesterase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PMEI1 PE=1 SV=1)

HSP 1 Score: 58.5 bits (140), Expect = 9.1e-08
Identity = 45/168 (26.79%), Postives = 83/168 (49.40%), Query Frame = 0


Query: 16  AFLCCLLFFNVVLLHADTEPSNDVASIVCPETRNRSLCWKVLKPA-GSTDALMVANYTLS 75
           AFL  L+F  ++L+ +    ++   S +C +T N S C K L     S +   +A  TL 
Sbjct: 9   AFLSSLMF--LLLIGSSYAITSSEMSTICDKTLNPSFCLKFLNTKFASPNLQALAKTTLD 68

Query: 76  LAHTSAVENLRLARRLEAAETANPLLEKRYSTCSRRLDFAVMGIEQGKEALATGGYILLK 135
                A + L+  + +      +P  +  Y +C    + A+  +E+  E LA+G  + + 
Sbjct: 69  STQARATQTLKKLQSI-IDGGVDPRSKLAYRSCVDEYESAIGNLEEAFEHLASGDGMGMN 128

Query: 136 VAISAAVVDANECLDAFKQPPRELSSLPEKAKNLGDICDIAVSVSTIL 183
           + +SAA+  A+ CLD  K+     SS+   +K + ++C IA+ +S +L
Sbjct: 129 MKVSAALDGADTCLDDVKRLRSVDSSVVNNSKTIKNLCGIALVISNML 173

[Close]

BLAST of Tan0008309 vs. NCBI nr
Match: XP_004148232.1 (pectinesterase inhibitor [Cucumis sativus])

HSP 1 Score: 204.1 bits (518), Expect = 1.0e-48
Identity = 111/185 (60.00%), Postives = 133/185 (71.89%), Query Frame = 0


Query: 3   NFNFNLGVFSSIQAFLCCLLFFNVVLLHADTEPSNDVASIVCPETRNRSLCWKVLKPAGS 62
           +FN NLGV SSI  FL  L FF+VVLLHADT   N + + +CP+TRN + C  VLKP G 
Sbjct: 9   SFNSNLGVVSSILIFLFGLFFFSVVLLHADTGSPNVLTTTICPKTRNSTFCKTVLKPVGK 68

Query: 63  TDA--LMVANYTLSLAHTSAVENLRLARRLEAAETANPLLEKRYSTCSRRLDFAVMGIEQ 122
           +DA  L VANYTL+ AHT+ VE L  A+RL A E  +PLL++RYS CSRR DF   G+E+
Sbjct: 69  SDAALLKVANYTLTFAHTTTVEGLHHAQRL-ATEATDPLLKQRYSECSRRFDFVTKGLEE 128

Query: 123 GKEALATGGYILLKVAISAAVVDANECLDAFKQPPRELSSLPEKAKNLGDICDIAVSVST 182
             EALA GGYI L  A  AAVV+A+ C++ FK+PP E S LPEKAKN+GDICDIAVSVS 
Sbjct: 129 AIEALAKGGYIPLSHATGAAVVEADRCVNMFKKPPPEPSKLPEKAKNIGDICDIAVSVSN 188

Query: 183 ILAGD 186
           IL  D
Sbjct: 189 ILTED 192

[Close]

BLAST of Tan0008309 vs. NCBI nr
Match: XP_008458686.1 (PREDICTED: pectinesterase inhibitor-like [Cucumis melo] >TYK02951.1 pectinesterase inhibitor-like [Cucumis melo var. makuwa])

HSP 1 Score: 196.4 bits (498), Expect = 2.1e-46
Identity = 110/185 (59.46%), Postives = 132/185 (71.35%), Query Frame = 0


Query: 3   NFNFNLGVFSSIQAFLCCLLFFNVVLLHADTEPSNDVASIVCPETRNRSLCWKVLKPAGS 62
           +FN NLGV SSI  FL  L FF+VVLLHADT   N + S +CP+TRN +LC  VLKP G 
Sbjct: 10  SFNSNLGVVSSILIFLFGLFFFSVVLLHADTGSPNVITSTICPKTRNSTLCKTVLKPVGK 69

Query: 63  TDA--LMVANYTLSLAHTSAVENLRLARRLEAAETANPLLEKRYSTCSRRLDFAVMGIEQ 122
           +DA  L VANY+L+LAHT+ VE L+ A+RL A +  +PLL++RYS CSRR D    G+E+
Sbjct: 70  SDAALLKVANYSLTLAHTTTVEGLQHAQRL-ANDATDPLLKQRYSECSRRFDSMTKGLEE 129

Query: 123 GKEALATGGYILLKVAISAAVVDANECLDAFKQPPRELSSLPEKAKNLGDICDIAVSVST 182
              ALA G YI L  A  AAVVDA+ C+D FK+ P E S LPEKAKN+GDICDIAVSVS 
Sbjct: 130 AIAALAKGEYIPLSHATGAAVVDADGCVDMFKKRPPEPSKLPEKAKNIGDICDIAVSVSN 189

Query: 183 ILAGD 186
           IL  D
Sbjct: 190 ILTED 193

[Close]

BLAST of Tan0008309 vs. NCBI nr
Match: XP_023548317.1 (pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 184.9 bits (468), Expect = 6.4e-43
Identity = 94/180 (52.22%), Postives = 121/180 (67.22%), Query Frame = 0


Query: 8   LGVFSSIQAFLCCLLFFNVVLLHADTEPSNDVASIVCPETRNRSLCWKVLKPAGSTDALM 67
           +GVFS ++  LCC+LFF +VL HADT P ND  S +CP+TRN SLC ++LKP  + +A  
Sbjct: 1   MGVFSYVKLSLCCVLFFGIVLSHADTTPQNDTVSTICPQTRNPSLCRQLLKPVSNLNATN 60

Query: 68  VANYTLSLAHTSAVENLRLARRLEAAETANPLLEKRYSTCSRRLDFAVMGIEQGKEALAT 127
           +ANY + LAHT+AVE  R  +RL+A ET N  L+KRYS C + +D +V  IE GK+ +  
Sbjct: 61  MANYMIDLAHTNAVECRRRVQRLKAEETTNLELKKRYSECWQCIDSSVSAIESGKQYMEY 120

Query: 128 GGYILLKVAISAAVVDANECLDAFKQPPRELSSLPEKAKNLGDICDIAVSVSTILAGDDY 187
             YI L + I  A    ++CLD F QPP E SSLP+  KNLGDIC I   V+ ILAGDDY
Sbjct: 121 KNYIALTIQIIGAGSHTDQCLDTFNQPPPEPSSLPKNVKNLGDICYITSYVTHILAGDDY 180

[Close]

BLAST of Tan0008309 vs. NCBI nr
Match: KAE8647076.1 (hypothetical protein Csa_023043 [Cucumis sativus])

HSP 1 Score: 172.6 bits (436), Expect = 3.3e-39
Identity = 91/156 (58.33%), Postives = 111/156 (71.15%), Query Frame = 0


Query: 32  DTEPSNDVASIVCPETRNRSLCWKVLKPAGSTDA--LMVANYTLSLAHTSAVENLRLARR 91
           DT   N + + +CP+TRN + C  VLKP G +DA  L VANYTL+ AHT+ VE L  A+R
Sbjct: 2   DTGSPNVLTTTICPKTRNSTFCKTVLKPVGKSDAALLKVANYTLTFAHTTTVEGLHHAQR 61

Query: 92  LEAAETANPLLEKRYSTCSRRLDFAVMGIEQGKEALATGGYILLKVAISAAVVDANECLD 151
           L A E  +PLL++RYS CSRR DF   G+E+  EALA GGYI L  A  AAVV+A+ C++
Sbjct: 62  L-ATEATDPLLKQRYSECSRRFDFVTKGLEEAIEALAKGGYIPLSHATGAAVVEADRCVN 121

Query: 152 AFKQPPRELSSLPEKAKNLGDICDIAVSVSTILAGD 186
            FK+PP E S LPEKAKN+GDICDIAVSVS IL  D
Sbjct: 122 MFKKPPPEPSKLPEKAKNIGDICDIAVSVSNILTED 156

[Close]

BLAST of Tan0008309 vs. NCBI nr
Match: XP_022958860.1 (pectinesterase inhibitor-like [Cucurbita moschata])

HSP 1 Score: 164.9 bits (416), Expect = 6.9e-37
Identity = 83/179 (46.37%), Postives = 112/179 (62.57%), Query Frame = 0


Query: 8   LGVFSSIQAFLCCLLFFNVVLLHADTEPSNDVASIVCPETRNRSLCWKVLKPAGSTDALM 67
           +GVFS +   LCC+LFF VVL HADT   ND  S +CP T N  LCW++LK   + D++ 
Sbjct: 1   MGVFSYVGFSLCCVLFFGVVLSHADTTLQNDTISTICPNTSNPLLCWQMLKSVRNLDSVS 60

Query: 68  VANYTLSLAHTSAVENLRLARRLEAAETANPLLEKRYSTCSRRLDFAVMGIEQGKEALAT 127
           + NYTL+   TSA+E L  A+ LEA ET N  L+KRYS CS+ +   V  IE  +E +  
Sbjct: 61  LTNYTLNFTRTSAIECLHYAQGLEAKETVNLELKKRYSYCSKSIKSTVTDIEVAQEYMGY 120

Query: 128 GGYILLKVAISAAVVDANECLDAFKQPPRELSSLPEKAKNLGDICDIAVSVSTILAGDD 187
           G YI+  +    A  D+ +CL++F QPP E S+L +  KNL DIC+IA   +  +AGDD
Sbjct: 121 GSYIVADLNTMGAFTDSGDCLNSFNQPPPEPSALQKSVKNLNDICEIACHFTHFIAGDD 179

[Close]

BLAST of Tan0008309 vs. ExPASy TrEMBL
Match: A0A0A0KHY0 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G307370 PE=4 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 5.0e-49
Identity = 111/185 (60.00%), Postives = 133/185 (71.89%), Query Frame = 0


Query: 3   NFNFNLGVFSSIQAFLCCLLFFNVVLLHADTEPSNDVASIVCPETRNRSLCWKVLKPAGS 62
           +FN NLGV SSI  FL  L FF+VVLLHADT   N + + +CP+TRN + C  VLKP G 
Sbjct: 9   SFNSNLGVVSSILIFLFGLFFFSVVLLHADTGSPNVLTTTICPKTRNSTFCKTVLKPVGK 68

Query: 63  TDA--LMVANYTLSLAHTSAVENLRLARRLEAAETANPLLEKRYSTCSRRLDFAVMGIEQ 122
           +DA  L VANYTL+ AHT+ VE L  A+RL A E  +PLL++RYS CSRR DF   G+E+
Sbjct: 69  SDAALLKVANYTLTFAHTTTVEGLHHAQRL-ATEATDPLLKQRYSECSRRFDFVTKGLEE 128

Query: 123 GKEALATGGYILLKVAISAAVVDANECLDAFKQPPRELSSLPEKAKNLGDICDIAVSVST 182
             EALA GGYI L  A  AAVV+A+ C++ FK+PP E S LPEKAKN+GDICDIAVSVS 
Sbjct: 129 AIEALAKGGYIPLSHATGAAVVEADRCVNMFKKPPPEPSKLPEKAKNIGDICDIAVSVSN 188

Query: 183 ILAGD 186
           IL  D
Sbjct: 189 ILTED 192

[Close]

BLAST of Tan0008309 vs. ExPASy TrEMBL
Match: A0A5D3BT93 (Pectinesterase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00330 PE=4 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 1.0e-46
Identity = 110/185 (59.46%), Postives = 132/185 (71.35%), Query Frame = 0


Query: 3   NFNFNLGVFSSIQAFLCCLLFFNVVLLHADTEPSNDVASIVCPETRNRSLCWKVLKPAGS 62
           +FN NLGV SSI  FL  L FF+VVLLHADT   N + S +CP+TRN +LC  VLKP G 
Sbjct: 10  SFNSNLGVVSSILIFLFGLFFFSVVLLHADTGSPNVITSTICPKTRNSTLCKTVLKPVGK 69

Query: 63  TDA--LMVANYTLSLAHTSAVENLRLARRLEAAETANPLLEKRYSTCSRRLDFAVMGIEQ 122
           +DA  L VANY+L+LAHT+ VE L+ A+RL A +  +PLL++RYS CSRR D    G+E+
Sbjct: 70  SDAALLKVANYSLTLAHTTTVEGLQHAQRL-ANDATDPLLKQRYSECSRRFDSMTKGLEE 129

Query: 123 GKEALATGGYILLKVAISAAVVDANECLDAFKQPPRELSSLPEKAKNLGDICDIAVSVST 182
              ALA G YI L  A  AAVVDA+ C+D FK+ P E S LPEKAKN+GDICDIAVSVS 
Sbjct: 130 AIAALAKGEYIPLSHATGAAVVDADGCVDMFKKRPPEPSKLPEKAKNIGDICDIAVSVSN 189

Query: 183 ILAGD 186
           IL  D
Sbjct: 190 ILTED 193

[Close]

BLAST of Tan0008309 vs. ExPASy TrEMBL
Match: A0A1S3C7Z4 (pectinesterase inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103498015 PE=4 SV=1)

HSP 1 Score: 196.4 bits (498), Expect = 1.0e-46
Identity = 110/185 (59.46%), Postives = 132/185 (71.35%), Query Frame = 0


Query: 3   NFNFNLGVFSSIQAFLCCLLFFNVVLLHADTEPSNDVASIVCPETRNRSLCWKVLKPAGS 62
           +FN NLGV SSI  FL  L FF+VVLLHADT   N + S +CP+TRN +LC  VLKP G 
Sbjct: 10  SFNSNLGVVSSILIFLFGLFFFSVVLLHADTGSPNVITSTICPKTRNSTLCKTVLKPVGK 69

Query: 63  TDA--LMVANYTLSLAHTSAVENLRLARRLEAAETANPLLEKRYSTCSRRLDFAVMGIEQ 122
           +DA  L VANY+L+LAHT+ VE L+ A+RL A +  +PLL++RYS CSRR D    G+E+
Sbjct: 70  SDAALLKVANYSLTLAHTTTVEGLQHAQRL-ANDATDPLLKQRYSECSRRFDSMTKGLEE 129

Query: 123 GKEALATGGYILLKVAISAAVVDANECLDAFKQPPRELSSLPEKAKNLGDICDIAVSVST 182
              ALA G YI L  A  AAVVDA+ C+D FK+ P E S LPEKAKN+GDICDIAVSVS 
Sbjct: 130 AIAALAKGEYIPLSHATGAAVVDADGCVDMFKKRPPEPSKLPEKAKNIGDICDIAVSVSN 189

Query: 183 ILAGD 186
           IL  D
Sbjct: 190 ILTED 193

[Close]

BLAST of Tan0008309 vs. ExPASy TrEMBL
Match: A0A6J1H4N5 (pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111460015 PE=4 SV=1)

HSP 1 Score: 164.9 bits (416), Expect = 3.3e-37
Identity = 83/179 (46.37%), Postives = 112/179 (62.57%), Query Frame = 0


Query: 8   LGVFSSIQAFLCCLLFFNVVLLHADTEPSNDVASIVCPETRNRSLCWKVLKPAGSTDALM 67
           +GVFS +   LCC+LFF VVL HADT   ND  S +CP T N  LCW++LK   + D++ 
Sbjct: 1   MGVFSYVGFSLCCVLFFGVVLSHADTTLQNDTISTICPNTSNPLLCWQMLKSVRNLDSVS 60

Query: 68  VANYTLSLAHTSAVENLRLARRLEAAETANPLLEKRYSTCSRRLDFAVMGIEQGKEALAT 127
           + NYTL+   TSA+E L  A+ LEA ET N  L+KRYS CS+ +   V  IE  +E +  
Sbjct: 61  LTNYTLNFTRTSAIECLHYAQGLEAKETVNLELKKRYSYCSKSIKSTVTDIEVAQEYMGY 120

Query: 128 GGYILLKVAISAAVVDANECLDAFKQPPRELSSLPEKAKNLGDICDIAVSVSTILAGDD 187
           G YI+  +    A  D+ +CL++F QPP E S+L +  KNL DIC+IA   +  +AGDD
Sbjct: 121 GSYIVADLNTMGAFTDSGDCLNSFNQPPPEPSALQKSVKNLNDICEIACHFTHFIAGDD 179

[Close]

BLAST of Tan0008309 vs. ExPASy TrEMBL
Match: A0A5D3BTG4 (Pectinesterase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00350 PE=4 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 4.0e-30
Identity = 81/185 (43.78%), Postives = 115/185 (62.16%), Query Frame = 0


Query: 1   MGNFNFNLGVFSS--IQAFLCCLLFFNVVLLHADT-EPSNDVASIVCPETRNRSLCWKVL 60
           M N +F+L +F S  I  F+C  LF NVV   A+T  PSNDVAS +CP+TRN S C  VL
Sbjct: 1   MTNSSFDLRIFGSVAILVFVCSFLFSNVVSSRANTITPSNDVASSICPKTRNPSFCVDVL 60

Query: 61  KPAGSTDALMVANYTLSLAHTSAVENLRLARRLEAAETANPLLEKRYSTCSRRLDFAVMG 120
           K AGSTD  ++A YTL+L +T+A ++L L + L AA T NP L+ +Y +C    +  V  
Sbjct: 61  KSAGSTDLKVLATYTLNLTNTNAEKSLNLVKSL-AATTTNPQLKNQYLSCYESYEEVVGD 120

Query: 121 IEQGKEALATGGYILLKVAISAAVVDANECLDAFKQPPRELSSLPEKAKNLGDICDIAVS 180
           IE  K  LA+G +  + +A S  +   ++C D+FKQPP++ S L +  K L D+C I + 
Sbjct: 121 IEIAKSNLASGDFNGVNIATSGIMTAVDDCQDSFKQPPKDTSMLLKNGKTLNDVCSIILV 180

Query: 181 VSTIL 183
           +S +L
Sbjct: 181 ISNLL 184

[Close]

BLAST of Tan0008309 vs. TAIR 10
Match: AT3G17220.1 (pectin methylesterase inhibitor 2 )

HSP 1 Score: 62.4 bits (150), Expect = 4.5e-10
Identity = 43/146 (29.45%), Postives = 70/146 (47.95%), Query Frame = 0


Query: 43  VCPETRNRSLCWKVLKPAGST---DALMVANYTLSLAHTSAVENLRLARRLEAAETANPL 102
           +C + +N+S C   +K    T   D   +AN T   A TSA E  R  + L    T NP 
Sbjct: 33  ICGKAKNQSFCTSYMKSNPKTSGADLQTLANITFGSAQTSASEGFRKIQSLVKTAT-NPT 92

Query: 103 LEKRYSTCSRRLDFAVMGIEQGKEALATGGYILLKVAISAAVVDANECLDAFKQPPRELS 162
           ++K Y++C +    A+  +   K++LA+G    L + +SAA+   + C    +Q   +  
Sbjct: 93  MKKAYTSCVQHYKSAISSLNDAKQSLASGDGKGLNIKVSAAMEGPSTC----EQDMADFK 152

Query: 163 SLPEKAKNLGD---ICDIAVSVSTIL 183
             P   KN GD   IC I + +S ++
Sbjct: 153 VDPSAVKNSGDFQNICGIVLVISNMM 173

[Close]

BLAST of Tan0008309 vs. TAIR 10
Match: AT1G48020.1 (pectin methylesterase inhibitor 1 )

HSP 1 Score: 58.5 bits (140), Expect = 6.5e-09
Identity = 45/168 (26.79%), Postives = 83/168 (49.40%), Query Frame = 0


Query: 16  AFLCCLLFFNVVLLHADTEPSNDVASIVCPETRNRSLCWKVLKPA-GSTDALMVANYTLS 75
           AFL  L+F  ++L+ +    ++   S +C +T N S C K L     S +   +A  TL 
Sbjct: 9   AFLSSLMF--LLLIGSSYAITSSEMSTICDKTLNPSFCLKFLNTKFASPNLQALAKTTLD 68

Query: 76  LAHTSAVENLRLARRLEAAETANPLLEKRYSTCSRRLDFAVMGIEQGKEALATGGYILLK 135
                A + L+  + +      +P  +  Y +C    + A+  +E+  E LA+G  + + 
Sbjct: 69  STQARATQTLKKLQSI-IDGGVDPRSKLAYRSCVDEYESAIGNLEEAFEHLASGDGMGMN 128

Query: 136 VAISAAVVDANECLDAFKQPPRELSSLPEKAKNLGDICDIAVSVSTIL 183
           + +SAA+  A+ CLD  K+     SS+   +K + ++C IA+ +S +L
Sbjct: 129 MKVSAALDGADTCLDDVKRLRSVDSSVVNNSKTIKNLCGIALVISNML 173

[Close]

BLAST of Tan0008309 vs. TAIR 10
Match: AT3G05741.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 42.7 bits (99), Expect = 3.7e-04
Identity = 31/140 (22.14%), Postives = 63/140 (45.00%), Query Frame = 0


Query: 46  ETRNRSLCWKVLKPAGSTDALMVANY-TLSLAHTS--AVENLRLARRLEAAETANPLLEK 105
           E  +R  C K       T ++ V  + +L +  T    ++   L  +L  +   +  L+ 
Sbjct: 51  EMFDRQFCAKWFNADQKTKSISVQGFISLRVKETREFGLQTQALMSKLAKSSGKDQQLKG 110

Query: 106 RYSTCSRRLDFAVMGIEQGKEALATGGYILLKVAISAAVVDANECLDAFKQPPRELSSLP 165
            Y +C      A+  +E+ ++ L++  +    VAIS A   A +C DAF+ P  E   + 
Sbjct: 111 SYESCVASYGQAIKELEKAQKFLSSNSFTQALVAISDAFYKAGDCKDAFEGPSNEPPLVF 170

Query: 166 EKAKNLGDICDIAVSVSTIL 183
            + +N  ++C +  S  +++
Sbjct: 171 NRVENFANMCYVTWSFGSLI 190

The following BLAST results are available for this feature:

BLAST of Tan0008309 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P83326	4.1e-16	30.23	Pectinesterase inhibitor OS=Actinidia deliciosa OX=3627 GN=PMEI PE=1 SV=2	[more]
Q9LUV1	6.3e-09	29.45	Pectinesterase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PMEI2 PE=1 SV=1	[more]
Q9LNF2	9.1e-08	26.79	Pectinesterase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PMEI1 PE=1 SV=1	[more]

BLAST of Tan0008309 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004148232.1	1.0e-48	60.00	pectinesterase inhibitor [Cucumis sativus]	[more]
XP_008458686.1	2.1e-46	59.46	PREDICTED: pectinesterase inhibitor-like [Cucumis melo] >TYK02951.1 pectinestera...	[more]
XP_023548317.1	6.4e-43	52.22	pectinesterase inhibitor-like [Cucurbita pepo subsp. pepo]	[more]
KAE8647076.1	3.3e-39	58.33	hypothetical protein Csa_023043 [Cucumis sativus]	[more]
XP_022958860.1	6.9e-37	46.37	pectinesterase inhibitor-like [Cucurbita moschata]	[more]

BLAST of Tan0008309 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KHY0	5.0e-49	60.00	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G307370 PE=4 S...	[more]
A0A5D3BT93	1.0e-46	59.46	Pectinesterase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]
A0A1S3C7Z4	1.0e-46	59.46	pectinesterase inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103498015 PE=4 SV=1	[more]
A0A6J1H4N5	3.3e-37	46.37	pectinesterase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111460015 PE=4...	[more]
A0A5D3BTG4	4.0e-30	43.78	Pectinesterase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc...	[more]

BLAST of Tan0008309 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G17220.1	4.5e-10	29.45	pectin methylesterase inhibitor 2	[more]
AT1G48020.1	6.5e-09	26.79	pectin methylesterase inhibitor 1	[more]
AT3G05741.1	3.7e-04	22.14	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 35..178 e-value: 9.5E-9 score: 45.1
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 15..182 e-value: 1.4E-16 score: 58.3
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 43..176 e-value: 1.1E-10 score: 42.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 36..183 e-value: 6.6E-19 score: 70.4
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 43..182
None	No IPR available	PANTHER	PTHR36710	PECTINESTERASE INHIBITOR-LIKE	coord: 15..182
None	No IPR available	PANTHER	PTHR36710:SF6	PECTINESTERASE INHIBITOR-LIKE	coord: 15..182
IPR034086	Pectinesterase inhibitor, plant	CDD	cd15797	PMEI	coord: 41..182 e-value: 2.17157E-26 score: 96.3373

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0008309.1	Tan0008309.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
cellular_component	GO:0016020	membrane
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity