Tan0008256 (gene) Snake gourd v1

BLAST of Tan0008256 vs. ExPASy Swiss-Prot
Match: F4HQ23 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.7 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-2.7 PE=3 SV=3)

HSP 1 Score: 426.0 bits (1094), Expect = 7.3e-118
Identity = 248/615 (40.33%), Postives = 364/615 (59.19%), Query Frame = 0

Query: 31  DLFKACEMSYNCGNLVSISYPFWGNARPDFCGSSKFELKCNNNKTTTIKINSLDYYVLNI 90
           +L + C   ++CGN  ++ YPFW   R ++CG   F+L C+      + I S+++ +LN+
Sbjct: 247 ELRRRCSERFSCGNQRNLYYPFWIPGR-EYCGHPDFKLDCSGG-FAELNIASVNFRILNM 306

Query: 91  NQSDQTMRMARSDLFDDYCPKSKIETAKLDPSQFSYTEKDLNISIWYDCPMQEGVPEEFR 150
           +      R+ARSD  +D CP + +    L  +   ++     ++++YDC +       F 
Sbjct: 307 SYDSSNKRLARSDYLNDLCPPNPLNDEPLIETVLQFSADTELLTLYYDCQLNSSATSPFP 366

Query: 151 FRCSGE-GEKGNTNNYALEPEAMYLSRSIRG--------CKVKMEATVSKKSFKEGEMN- 210
               GE G     + Y     +  L    RG        CK K+    S  +    + N 
Sbjct: 367 SSYFGELGCDEGRSYYVTRNLSSPLLDRFRGVLNNLREMCKRKVSVPASGPALNTLQTNP 426

Query: 211 RTTLVETALREGFEVQYADDSGCYFCKENGGACGGNATQD-YYCICENG--------NIN 270
            +  ++ AL  GFE+Q  D+S C  C  +GG+CG N     + C C++G        N++
Sbjct: 427 NSNNLKMALEHGFELQ--DNSNCSMCVLSGGSCGYNQNSSLFVCYCKDGPQDNQCDINLD 486

Query: 271 SFSCSSRIHTPASVGIAIGSI-NLIIFIVILSILLLVR-WRKKKKRNSKKDEIEETIKRY 330
               +  +     + +A+ +I   I F+V+L     VR +R +K  + ++ E      + 
Sbjct: 487 DIIGNRIVAIIIGIFVALCTIGGFIAFLVLLCPCCKVRIFRNRKTSDDRRQE------KL 546

Query: 331 STQIP-KRYTYSELKKITDSFKNKLGQGGFSIVYKGKLLDGRYVAVKLLNESR-EDAQDF 390
              IP K YTY+++K++T SF   +G+GGF IVY+G L DGR VAVK+L ES+  +++DF
Sbjct: 547 KALIPLKHYTYAQVKRMTKSFAEVVGRGGFGIVYRGTLCDGRMVAVKVLKESKGNNSEDF 606

Query: 391 INEVVSITRTSHVNIVTFLGFCYEENKRALIYEYMPKGSLDKYIFQKGLQEKNKAKLNWN 450
           INEV S+++TSHVNIV+ LGFC E ++RA+IYE++  GSLDK+I      EK    L+  
Sbjct: 607 INEVSSMSQTSHVNIVSLLGFCSEGSRRAIIYEFLENGSLDKFI-----SEKTSVILDLT 666

Query: 451 TLYSIIVGVARGLEYLHRGCNTRILHFDIKPHNILLDNEFCPKISDFGLAKQCKARESHV 510
            LY I +GVARGLEYLH GC TRI+HFDIKP N+LLD+   PK+SDFGLAK C+ +ES +
Sbjct: 667 ALYGIALGVARGLEYLHYGCKTRIVHFDIKPQNVLLDDNLSPKVSDFGLAKLCEKKESVM 726

Query: 511 SMTCAKGTIGFMAPEVIIRSFGKVSHKSDVYSYGMLVLEMVGERKSPNKGESSEE----Y 570
           S+   +GTIG++APE+I R +G VSHKSDVYSYGMLV EM+G RK    G++S      Y
Sbjct: 727 SLMDTRGTIGYIAPEMISRVYGSVSHKSDVYSYGMLVFEMIGARKKERFGQNSANGSSMY 786

Query: 571 FPDWIYKDLAGSEI-DRNCRQWGQTEEEEEMVRKMVVVSLYCIQTLPDDRPSMTEVVAML 618
           FP+WIYKDL  ++  D    + G + EEEE+ +KM +V L+CIQ+ P DRP M +VV M+
Sbjct: 787 FPEWIYKDLEKADNGDLEHIEIGISSEEEEIAKKMTLVGLWCIQSSPSDRPPMNKVVEMM 846

BLAST of Tan0008256 vs. ExPASy Swiss-Prot
Match: F4HQ22 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.4 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-2.4 PE=3 SV=1)

HSP 1 Score: 415.2 bits (1066), Expect = 1.3e-114
Identity = 252/630 (40.00%), Postives = 362/630 (57.46%), Query Frame = 0

Query: 9   IFFFISFIAFQLSPCFSNDAGLDLFKACEMSYNCGNLVSISYPFWGNARPDFCGSSKFEL 68
           +  F+ F  F   PC S+   L     CE  + CGN ++  +PFWG  RP+ CG    EL
Sbjct: 9   LVLFLFFSLFYHLPCASSKQTLGW---CESQFQCGN-ITAGFPFWGGNRPEVCGHPLLEL 68

Query: 69  KCNNNKTTTIKINSLDYYVLNINQSDQTMRMARSDLFDDYC-PKSKIETAKLDPSQFSYT 128
            C +N  T++ I+   Y+VL+IN +  T+R+AR+D     C        A L P  F   
Sbjct: 69  HCLDN-ITSLTISDHLYHVLSINHTYNTLRVARTDFLQSICLSPFPFANATLPPEIFDIL 128

Query: 129 EKDLNISIWYDCPMQEGVPEEFRFRCSGEGEKGNTNNYALEPEAMYLSRSIRGCKVKMEA 188
               +++++   P+   +P+  R+ C   G    ++N         L   +  C+ +   
Sbjct: 129 PTYKSVTLYRCYPV---IPDLARYGCPAIGSVSVSDN---------LENPV-SCEARFTV 188

Query: 189 TVSKKSF-KEGEMNRTTLVETALREGFEVQY-ADDSGCYFCKENGGACGGNATQDYYCIC 248
            +       E  +N T+LV   +R GFEV+   D++ C  C  +   CG   T      C
Sbjct: 189 NIPTSFVPNEKRLNITSLVRD-VRNGFEVRLRIDENSCQECSSSHKYCGFTGTLPLETKC 248

Query: 249 ENGNINSFSCSSRIHTPASVGIAIGSINLIIFIVILSILLLVRWRKKKKRNSKKDEIEET 308
              N+      +R+ + A +   I  ++L+ F+V+  ++ ++R +K      ++D  E  
Sbjct: 249 RPLNL-----PTRLSSEAKIA-TIAGVSLLPFLVLTLVVHIIRKQKTSNDKGQQDLKEHI 308

Query: 309 IK-RYSTQIP-KRYTYSELKKITDSFKNKLGQGGFSIVYKGKLLDGRYVAVKLLNESR-E 368
            K R    I  K+Y+Y ++K+IT+SF   +G+GGF IVY+G L DGR VAVK+L + +  
Sbjct: 309 PKPRIKALIQLKQYSYEQVKRITNSFAEVVGRGGFGIVYRGTLSDGRMVAVKVLKDLKGN 368

Query: 369 DAQDFINEVVSITRTSHVNIVTFLGFCYEENKRALIYEYMPKGSLDKYIFQKGLQEKNKA 428
           + +DFINEV S+++TSHVNIVT LGFC E  KRA+IYE+M  GSLDK+I       K  +
Sbjct: 369 NGEDFINEVASMSQTSHVNIVTLLGFCSEGYKRAIIYEFMENGSLDKFI-----SSKKSS 428

Query: 429 KLNWNTLYSIIVGVARGLEYLHRGCNTRILHFDIKPHNILLDNEFCPKISDFGLAKQCKA 488
            ++W  LY I +GVARGLEYLH GC TRI+HFDIKP N+LLD+   PK+SDFGLAK C+ 
Sbjct: 429 TMDWRELYGIALGVARGLEYLHHGCRTRIVHFDIKPQNVLLDDNLSPKVSDFGLAKLCER 488

Query: 489 RESHVSMTCAKGTIGFMAPEVIIRSFGKVSHKSDVYSYGMLVLEMVGERKSPN----KGE 548
           +ES +S+   +GTIG++APEV  R +G VSHKSDVYSYGMLVL+++G R   +       
Sbjct: 489 KESILSLMDTRGTIGYIAPEVFSRVYGSVSHKSDVYSYGMLVLDIIGARNKTSTEDTTSS 548

Query: 549 SSEEYFPDWIYKDLAGSEIDRNCRQWGQTEEEEEMVRKMVVVSLYCIQTLPDDRPSMTEV 608
           +S  YFP+WIYKDL   E   N R      EE+E+ +KM +V L+CIQ  P DRP+M  V
Sbjct: 549 TSSMYFPEWIYKDL---EKGDNGRLIVNRSEEDEIAKKMTLVGLWCIQPWPLDRPAMNRV 605

Query: 609 VAMLEGSADGLQIPPKPVLFESPAASLSSS 629
           V M+EG+ D L++PP+PVL  S    L SS
Sbjct: 609 VEMMEGNLDALEVPPRPVLQCSVVPHLDSS 605

BLAST of Tan0008256 vs. ExPASy Swiss-Prot
Match: Q3ECH2 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.8 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-2.8 PE=2 SV=2)

HSP 1 Score: 407.1 bits (1045), Expect = 3.5e-112
Identity = 253/634 (39.91%), Postives = 371/634 (58.52%), Query Frame = 0

Query: 31  DLFKACEMSYNCGNLVSISYPFWGNARPDFCGSSKFELKCNNNKTTTIKINSLDYYVLNI 90
           ++++ C  S++CG+   ++YP W   R + CG   F+L C+      I I S+ + +L+ 
Sbjct: 249 EVYRRCSESFSCGSQRDLNYPLWKPGREE-CGHPNFKLNCSGG-FAEINIASVKFRILDS 308

Query: 91  NQSDQTMRMARSDLFDDYCPKSKIETAKLDP--SQFSYTEKDLNISIWYDCPMQEG-VPE 150
            +S   +R+ARSD   D CP + +    ++        T +   ++++Y C      +P 
Sbjct: 309 YRS--LIRLARSDYIGDLCPANPLTAPFIEKFLPVLELTGETELLTLYYGCRFNSSDIPA 368

Query: 151 EFRFRCSG--EGEK----GNTNNYALEPEAMYLSRSIRGCKVKMEATVSKKSF--KEGEM 210
                  G  EG       N ++  L+     L+     CK  +    S  +    +   
Sbjct: 369 NIYVGELGCDEGRSYYVTRNLSSPLLDSSRGVLNNLREMCKRNVSVPASGPALFDLQTRP 428

Query: 211 NRTTLVETALREGFEVQYADDSGCYFCKENGGACGGNATQD-YYCICENGNI-----NSF 270
           N+  L + AL +GF +    D  C  C+ +GGACG N T   + C C++G       + F
Sbjct: 429 NQDNL-KMALDQGFRMLITSD--CERCRGSGGACGYNQTSSGFGCYCKDGKCGYEYDDGF 488

Query: 271 SCSSRIHTPASVG---IAIGSIN--LIIFIVILSILLLVRWRKKKKRNSKKDEIEETIKR 330
               R      V    IA+G++   +I+F+V+L     V+  +K+K +   DE+   +++
Sbjct: 489 FRRHRRFIATLVRYTFIALGALTGVVIVFLVLLCPCFRVQIFRKRKTS---DEVR--LQK 548

Query: 331 YSTQIP-KRYTYSELKKITDSFKNKLGQGGFSIVYKGKLLDGRYVAVKLLNESR-EDAQD 390
               IP K YTY+E+KK+T SF   +G+GGF IVY G L D   VAVK+L +S+  D +D
Sbjct: 549 LKALIPLKHYTYAEVKKMTKSFTEVVGRGGFGIVYSGTLSDSSMVAVKVLKDSKGTDGED 608

Query: 391 FINEVVSITRTSHVNIVTFLGFCYEENKRALIYEYMPKGSLDKYIFQKGLQEKNKAKLNW 450
           FINEV S+++TSHVNIV+ LGFC E ++RA+IYE++  GSLDK+I      +K+   L+ 
Sbjct: 609 FINEVASMSQTSHVNIVSLLGFCCEGSRRAIIYEFLGNGSLDKFI-----SDKSSVNLDL 668

Query: 451 NTLYSIIVGVARGLEYLHRGCNTRILHFDIKPHNILLDNEFCPKISDFGLAKQCKARESH 510
            TLY I +GVARGLEYLH GC TRI+HFDIKP N+LLD+  CPK+SDFGLAK C+ +ES 
Sbjct: 669 KTLYGIALGVARGLEYLHYGCKTRIVHFDIKPQNVLLDDNLCPKVSDFGLAKLCEKKESI 728

Query: 511 VSMTCAKGTIGFMAPEVIIRSFGKVSHKSDVYSYGMLVLEMVGERKSP-----NKGESSE 570
           +S+   +GTIG++APE+I R +G VSHKSDVYSYGMLVLEM+G RK       ++ + S 
Sbjct: 729 LSLLDTRGTIGYIAPEMISRLYGSVSHKSDVYSYGMLVLEMIGARKKERFDQNSRSDGSS 788

Query: 571 EYFPDWIYKDLAGSEI-------DRNCRQWGQTEEEEEMVRKMVVVSLYCIQTLPDDRPS 629
            YFP+WIYKDL  + I       +    + G + EEEE+ RKM +V L+CIQ+ P DRP 
Sbjct: 789 IYFPEWIYKDLEKANIKDIEKTENGGLIENGISSEEEEIARKMTLVGLWCIQSSPSDRPP 848

BLAST of Tan0008256 vs. ExPASy Swiss-Prot
Match: Q9FF31 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.1 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-2.1 PE=3 SV=1)

HSP 1 Score: 396.7 bits (1018), Expect = 4.8e-109
Identity = 232/610 (38.03%), Postives = 358/610 (58.69%), Query Frame = 0

Query: 34  KACEMSYNCGNLVSISYPFWGNARPDFCGSSKFELKCNNNKTTTIKINSLDYYVLNINQS 93
           K C  ++ CG    + YPFW   R + CG   F++ C+ +      I+++ ++VL +N  
Sbjct: 35  KHCSPTFRCGKQTDLYYPFWSPDREE-CGHPVFKVNCSGD-FAEFTISTVKFHVLEMNYE 94

Query: 94  DQTMRMARSDLFDDYCPKSKIETAKLDPSQFSYTEKDLNISIWYDC--PMQEGVPEEFRF 153
            + +R+ R++  ++ CP    E   ++     + +     + +Y+C  P  + +   +  
Sbjct: 95  SRIIRLVRTEYLNNLCPWHP-ENRSINQEVLPFLQDTELGTFYYNCSGPTVDELANGYIR 154

Query: 154 RCSGEGEKGNTNNYALEP----EAMYLSRSIRGCKVKMEATVSKKSFKEGEMNRT-TLVE 213
           +   + E G  + +   P        L      C+  ++  VS+ + +    N++   ++
Sbjct: 155 QLGCDEEVGGKSYFVSSPSHPGNRAILDGLSASCERNVDIPVSRSAMETTATNQSLEAIK 214

Query: 214 TALREGFEVQYADDSGCYFCKENGGACGGN-ATQDYYCICENGNINSFSCSSRIHTPASV 273
             L  GFE+ +  +S C  C  + GACG N +++ + C C++            HT   +
Sbjct: 215 KVLDVGFELGF--NSDCSLCVASKGACGFNQSSKAFVCYCKD--------EPHEHTCGKM 274

Query: 274 GIAIGSINLIIFIVILSILLLVRWRKKKKRNSKKDEIEETIKRYSTQIPKRYTYSELKKI 333
           GI IG     +   ++++ LL  + +K++ +      +  +K    Q+ K+Y+Y+E++KI
Sbjct: 275 GIGIGLGCGFLGATLITVCLLCFFFQKRRTSHHLRPRDNNLKGL-VQL-KQYSYAEVRKI 334

Query: 334 TDSFKNKLGQGGFSIVYKGKLLDGRYVAVKLLNESREDAQDFINEVVSITRTSHVNIVTF 393
           T  F + LG+GGF  VY G L DGR VAVK+L + + + +DFINEV S+++TSHVNIV+ 
Sbjct: 335 TKLFSHTLGKGGFGTVYGGNLCDGRKVAVKILKDFKSNGEDFINEVASMSQTSHVNIVSL 394

Query: 394 LGFCYEENKRALIYEYMPKGSLDKYIFQKGLQEKNKAKLNWNTLYSIIVGVARGLEYLHR 453
           LGFCYE +KRA++YE++  GSLD++     L EK    L+ +TLY I +GVARGL+YLH 
Sbjct: 395 LGFCYEGSKRAIVYEFLENGSLDQF-----LSEKKSLNLDVSTLYRIALGVARGLDYLHH 454

Query: 454 GCNTRILHFDIKPHNILLDNEFCPKISDFGLAKQCKARESHVSMTCAKGTIGFMAPEVII 513
           GC TRI+HFDIKP NILLD+ FCPK+SDFGLAK C+ RES +S+  A+GTIG++APEV  
Sbjct: 455 GCKTRIVHFDIKPQNILLDDTFCPKVSDFGLAKLCEKRESILSLLDARGTIGYIAPEVFS 514

Query: 514 RSFGKVSHKSDVYSYGMLVLEMVG----ERKSPNKGESSEEYFPDWIYKDLAGSEIDRNC 573
             +G+VSHKSDVYSYGMLVLEM+G    E +      SS  YFPDWIYK+L   E   + 
Sbjct: 515 GMYGRVSHKSDVYSYGMLVLEMIGAKNKEIEETAASNSSSAYFPDWIYKNLENGE---DT 574

Query: 574 RQWGQ--TEEEEEMVRKMVVVSLYCIQTLPDDRPSMTEVVAMLEGSADGLQIPPKPVLFE 628
            ++G   + E++E+ +KM +V L+CIQ  P +RP M  +V M+EGS D L++PPKP +  
Sbjct: 575 WKFGDEISREDKEVAKKMTLVGLWCIQPSPLNRPPMNRIVEMMEGSLDVLEVPPKPSIHY 621

BLAST of Tan0008256 vs. ExPASy Swiss-Prot
Match: F4HQ20 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.5 OS=Arabidopsis thaliana OX=3702 GN=LRK10L-2.5 PE=3 SV=1)

HSP 1 Score: 396.4 bits (1017), Expect = 6.2e-109
Identity = 239/625 (38.24%), Postives = 353/625 (56.48%), Query Frame = 0

Query: 36  CEMSYNCGNLVSISYPFWGNARPDFCGSSKFELKCNNNKTTTIKINSLDYYVLNINQSDQ 95
           C  S+ CGN   + YPFW   R + CG   FEL CN      + I+S+ + +L  +    
Sbjct: 38  CSPSFTCGNQRGLLYPFWIAGRKE-CGHPDFELDCNAG-VPELSISSVKFRILGADYDSG 97

Query: 96  TMRMARSDLFDDYCPKSKIETAKLDPSQFSYTEKDLNISIWYDCPMQ-EGVPEEF--RFR 155
            + +ARSD  DD C  +   T+  +      +  DL ++I+YDC          F     
Sbjct: 98  IITLARSDNIDDPCLPNSFTTSFNETVLPLASTTDL-LTIYYDCNRNVSSFVSTFVKELD 157

Query: 156 CSGEGEKGNTNNY------------ALEPEAMYLSRSIRGCKVKMEATVSKKSFKEGEMN 215
           C  +G     N Y             LE  +  L+     C   +    S+ +    E  
Sbjct: 158 CPDDGTDDRRNYYLTRNLTFLPPSLKLEGNSFLLNDFGGSCSRNVSNPASRTALNTLEST 217

Query: 216 RTT-LVETALREGFEVQYADDSGCYFCKENGGACGGNATQDYYCIC------ENGNINSF 275
            +T  ++ AL +GF ++   +S C  C ++ GACG + T   + +C      +N   N  
Sbjct: 218 PSTDNLKIALEDGFALEV--NSDCRTCIDSKGACGFSQTSSRF-VCYYRQEPQNPTRNKV 277

Query: 276 SCSSRIHTPASVGIAIGSINLIIFIVILSILLLVRWRKKKKRNSKKDEIEETIKRYSTQ- 335
                      +GI   S  ++  I++++ L  +  R++K  N  +    +  ++ + + 
Sbjct: 278 ILKLFFIVIYVLGIGAASFAMMGVILVVTCLNCLIRRQRKTLNDPRMRTSDDSRQQNLKA 337

Query: 336 -IP-KRYTYSELKKITDSFKNKLGQGGFSIVYKGKLLDGRYVAVKLLNESREDAQDFINE 395
            IP K Y+Y+++  IT SF   +G+GGF  VY+G L DGR VAVK+L ES+ + +DFINE
Sbjct: 338 LIPLKHYSYAQVTSITKSFAEVIGKGGFGTVYRGTLYDGRSVAVKVLKESQGNGEDFINE 397

Query: 396 VVSITRTSHVNIVTFLGFCYEENKRALIYEYMPKGSLDKYIFQKGLQEKNKAKLNWNTLY 455
           V S+++TSHVNIVT LGFC E  KRA+IYE+M  GSLDK+I       K  + ++W  LY
Sbjct: 398 VASMSQTSHVNIVTLLGFCSEGYKRAIIYEFMENGSLDKFI-----SSKKSSTMDWRELY 457

Query: 456 SIIVGVARGLEYLHRGCNTRILHFDIKPHNILLDNEFCPKISDFGLAKQCKARESHVSMT 515
            I +GVARGLEYLH GC TRI+HFDIKP N+LLD+   PK+SDFGLAK C+ +ES +S+ 
Sbjct: 458 GIALGVARGLEYLHHGCRTRIVHFDIKPQNVLLDDNLSPKVSDFGLAKLCERKESILSLM 517

Query: 516 CAKGTIGFMAPEVIIRSFGKVSHKSDVYSYGMLVLEMVGERKSPN----KGESSEEYFPD 575
             +GTIG++APEV  R +G+VSHKSDVYSYGMLVL+++G R   +       +S  YFP+
Sbjct: 518 DTRGTIGYIAPEVFSRVYGRVSHKSDVYSYGMLVLDIIGARNKTSTEDTTSSTSSMYFPE 577

Query: 576 WIYKDLAGSEIDRNCRQWGQTEEEEEMVRKMVVVSLYCIQTLPDDRPSMTEVVAMLEGSA 632
           WIY+DL  +   ++  +   + EE+E+ +KM +V L+CIQ  P DRP+M  VV M+EG+ 
Sbjct: 578 WIYRDLEKAHNGKSI-ETAISNEEDEIAKKMTLVGLWCIQPWPLDRPAMNRVVEMMEGNL 637

BLAST of Tan0008256 vs. NCBI nr
Match: XP_022137293.1 (uncharacterized protein LOC111008790 [Momordica charantia])

HSP 1 Score: 794.3 bits (2050), Expect = 7.8e-226
Identity = 402/632 (63.61%), Postives = 478/632 (75.63%), Query Frame = 0

Query: 9   IFFFISFI---AFQLSPCFSNDAGLDLFKACEMSYNCGNLVSISYPFWGNARPDFCGSSK 68
           +FFF++F     +   P  S +  L  FKAC + YNCGNLV+ISYPFWGN R DFCG  +
Sbjct: 7   LFFFVAFFFSSVWSDLPLCSGEDALSEFKACGVDYNCGNLVNISYPFWGNERQDFCGRRE 66

Query: 69  FELKCNNNKTTTIKINSLDYYVLNINQSDQTMRMARSDLFDDYCPKSKIETAKLDPSQFS 128
           F L C  N TTTI+INSL+Y VL+INQ+D  M +ARSDLFDDYCPK++IETAK D    +
Sbjct: 67  FVLNCKQNLTTTIQINSLEYKVLHINQTDHRMTIARSDLFDDYCPKNQIETAKFDHHLLT 126

Query: 129 YTEKDLNISIWYDCPMQEGVPEEFRFRCSGEGEKGNTNNYALEPEAMYLSRSIRGCKVKM 188
           Y   D N+S WYDCP Q+G+P+EFRF C  EGE+    NYALEPE M LS +IRGCK+ +
Sbjct: 127 YYWYDRNMSFWYDCPPQQGIPDEFRFFCGWEGERSGRANYALEPEEMNLSGNIRGCKMNV 186

Query: 189 EATVSKKSFKEGEMNRTTLVETALREGFEVQYAD--DSGCYFCKENGGACGGNATQDYYC 248
           E  V+ +  +EG+ NRT  +E AL+ GF+V+Y D     C  CK++GG CG N T  +YC
Sbjct: 187 EVXVTIEGLEEGKENRTARLEKALQRGFDVEYGDLHTMACDVCKQSGGYCGANGTYPFYC 246

Query: 249 IC-ENGNINSFSCSSRIHTPAS--------VGIAIGSINLIIFIVILSILLLVRWRKKKK 308
           IC + G+ + + C+S   +  S        V +A GS   IIF+++  I +       + 
Sbjct: 247 ICGDRGDAHPYVCTSISTSRKSSFQTKLVIVAVASGS---IIFLILFMIFIYY----TRI 306

Query: 309 RNSKKDEIEETIKRYSTQIPKRYTYSELKKITDSFKNKLGQGGFSIVYKGKLLDGRYVAV 368
           R S K+EIEE ++R+ST IPKRY+YS+LKKIT SFKNKLGQGGFS VYKGKL DGR VAV
Sbjct: 307 RISNKEEIEEIVRRHSTHIPKRYSYSKLKKITHSFKNKLGQGGFSTVYKGKLPDGRDVAV 366

Query: 369 KLLNESREDAQDFINEVVSITRTSHVNIVTFLGFCYEENKRALIYEYMPKGSLDKYIFQK 428
           KLLNES+E+ QDFINEVVSIT TSHVNI T LGFCYE N+RAL+YEYMPKGSLDKYIF K
Sbjct: 367 KLLNESKENGQDFINEVVSITTTSHVNIATLLGFCYERNRRALVYEYMPKGSLDKYIFHK 426

Query: 429 GLQEKNKAKLNWNTLYSIIVGVARGLEYLHRGCNTRILHFDIKPHNILLDNEFCPKISDF 488
             Q K+   L+WNTLY+I++GVARGLEYLHRGCNTRILHFDIKPHNILLDNEFCPKISDF
Sbjct: 427 DPQ-KSDMGLDWNTLYTIVIGVARGLEYLHRGCNTRILHFDIKPHNILLDNEFCPKISDF 486

Query: 489 GLAKQCKARESHVSMTCAKGTIGFMAPEVIIRSFGKVSHKSDVYSYGMLVLEMVGERKSP 548
           GLAKQC+ARESHVSMT  KGTIGFMAPEV+ R+ GKVSHKSDVYSYGMLVLEMVGERK+P
Sbjct: 487 GLAKQCRARESHVSMTGVKGTIGFMAPEVVFRNIGKVSHKSDVYSYGMLVLEMVGERKNP 546

Query: 549 NK--GESSEEYFPDWIYKDLAGSEIDRNCRQWGQTEEEEEMVRKMVVVSLYCIQTLPDDR 608
           N+  G+ SEEYFPDWIYKDL  SE D  C  WG TEEE+EM RKM++V L+CIQTLPDDR
Sbjct: 547 NEGVGQRSEEYFPDWIYKDLTQSETDEGC-SWGNTEEEDEMARKMIIVGLHCIQTLPDDR 606

Query: 609 PSMTEVVAMLEGSADGLQIPPKPVLFESPAAS 625
           PSM++VV MLEGS DGLQIPPKP    +P  +
Sbjct: 607 PSMSDVVGMLEGSVDGLQIPPKPNFMGTPTTT 629

BLAST of Tan0008256 vs. NCBI nr
Match: XP_031743234.1 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.4 isoform X2 [Cucumis sativus])

HSP 1 Score: 790.4 bits (2040), Expect = 1.1e-224
Identity = 407/652 (62.42%), Postives = 493/652 (75.61%), Query Frame = 0

Query: 1   MKMSLEPNIFFFISFIAFQLSPCFSNDAGLDLFKACEMSYNCGNLVSISYPFWGNARPDF 60
           MK SL   +FF I +  F L  CFSNDA  + FKAC + Y+CG LV+ISYPFWGN R  F
Sbjct: 1   MKPSLPQFLFFTILWFDFHL--CFSNDAN-EEFKACSVYYSCGVLVNISYPFWGNERQQF 60

Query: 61  CGSSKFELKCNNNKTTTIKINSLDYYVLNINQSDQTMRMARSDLFDDYCPKSKIETAKLD 120
           CG  +FEL C +NKTTTI+INS++Y V+NINQ+D +M +ARSDL DDYCPK +I+TA L 
Sbjct: 61  CGRREFELNCKDNKTTTIQINSVEYNVVNINQTDHSMTIARSDLLDDYCPKIQIKTATLG 120

Query: 121 PSQFSYTEKDLNISIWYDCPMQEGVPEEFRFRC-SGEGEKGNTNNYALE-PEAMYLSRSI 180
            + F Y+  DLN+S+WYDCP+ EG+  E  F C S EGE+    NYALE  +AM   R++
Sbjct: 121 HNLFKYSSNDLNLSLWYDCPVLEGIRREMTFECGSSEGERRGRVNYALEKKDAMNWRRNM 180

Query: 181 RGCKVKMEATVSKKSFKEGEMNRTTLVETALREGFEVQYAD--DSGCYFCKENGGACGGN 240
             C+VKME T++K+   EGE NRT +VE  ++EGFEV+Y D     C  CKE GGACGGN
Sbjct: 181 SECRVKMEVTITKEVLTEGEKNRTMVVERGMKEGFEVEYGDLYTIACEGCKEQGGACGGN 240

Query: 241 ATQDYYCICENGNINSFSCSSRIHT------PASVGIAI-------GSINLIIFIVILSI 300
            ++++ C+C NGN++ + C S   +      PA+  + +       G  +   F++++SI
Sbjct: 241 TSKEFRCVCGNGNVHPYICKSSSSSSPPPPPPAAPYLDVIWLDTKTGGTSSGGFVLVISI 300

Query: 301 LLLVRWRKKKKRNSKKDEIEETIKRYSTQIPKRYTYSELKKITDSFKNKLGQGGFSIVYK 360
           ++ + W  K+KR S KD+IE+ I+RYS Q PKRY+YS+LKKITD F NKLGQGGFS VYK
Sbjct: 301 IIFIYW--KRKRTSNKDKIEKIIRRYSIQTPKRYSYSKLKKITDCFNNKLGQGGFSTVYK 360

Query: 361 GKLLDGRYVAVKLLNESR-EDAQDFINEVVSITRTSHVNIVTFLGFCYEENKRALIYEYM 420
           GKL +G  VAVKLLNESR E+ QDFINEVVSI +TSH+NIVT +GFCYE+NKRALIYEYM
Sbjct: 361 GKLPNGCDVAVKLLNESRQENGQDFINEVVSIAKTSHINIVTLIGFCYEQNKRALIYEYM 420

Query: 421 PKGSLDKYIFQKGLQEKNKAKLNWNTLYSIIVGVARGLEYLHRGCNTRILHFDIKPHNIL 480
           PKGSLDKYI+   LQE N  KL+WNTLY+I++GVARGLEYLHRGCNTRILHFDIKPHNIL
Sbjct: 421 PKGSLDKYIYHNRLQE-NDMKLDWNTLYNIVIGVARGLEYLHRGCNTRILHFDIKPHNIL 480

Query: 481 LDNEFCPKISDFGLAKQCKARESHVSMTCAKGTIGFMAPEVIIRSFGKVSHKSDVYSYGM 540
           LD++FCPKISDFGLAKQC+ARESHVSMT  KGTIGF+APEVI R+ GKVSHKSDVYSYGM
Sbjct: 481 LDSDFCPKISDFGLAKQCEARESHVSMTGVKGTIGFIAPEVIFRNSGKVSHKSDVYSYGM 540

Query: 541 LVLEMVGERKSPNKG--ESSEEYFPDWIYKDLAGSEIDRNCRQWGQTEEEEEMVRKMVVV 600
           L+LEMVG RK PN+G  + SE YFPDWIYKDL  SEID  C  WG T+EEEEM RKM++V
Sbjct: 541 LILEMVGARKKPNEGVEQKSEAYFPDWIYKDLTQSEIDGGC-WWGNTKEEEEMARKMIIV 600

Query: 601 SLYCIQTLPDDRPSMTEVVAMLEGSADGLQIPPKPVLF-----ESPAASLSS 628
            L+CIQTLPDDRPSMT VV MLEGS D LQIPPKP ++     E P AS SS
Sbjct: 601 GLHCIQTLPDDRPSMTNVVVMLEGSVDVLQIPPKPNMYGPPNIEQPQASFSS 645

BLAST of Tan0008256 vs. NCBI nr
Match: XP_038893810.1 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.4 [Benincasa hispida])

HSP 1 Score: 788.5 bits (2035), Expect = 4.3e-224
Identity = 397/626 (63.42%), Postives = 477/626 (76.20%), Query Frame = 0

Query: 11  FFISFIAFQLSPCFSNDAGLDLFKACEMSYNCGNLVSISYPFWGNARPDFCGSSKFELKC 70
           FF   + F L  CF +DA  + FK C + YNCG+LV+ISYPFWGN R DFCG  +F L C
Sbjct: 12  FFFRIVWFDLQLCFGDDAH-EEFKDCSVYYNCGDLVNISYPFWGNDRQDFCGRREFGLNC 71

Query: 71  NNNKTTTIKINSLDYYVLNINQSDQTMRMARSDLFDDYCPKSKIETAKLDPSQFSYTEKD 130
            +NKTTTI+INS++Y V+ I+QSD  + +ARSDLFDDYCP ++I+TA LD S F Y+  D
Sbjct: 72  KDNKTTTIQINSMEYNVVKISQSDHRLTIARSDLFDDYCPNNQIKTATLDHSPFKYSSND 131

Query: 131 LNISIWYDCPMQEGVPEEFRFRCSGEGEKGNTNNYALE-PEAMYLSRSIRGCKVKMEATV 190
           LN+S+WYDCP  EG   +FRF C  E E     NYALE  +AMY  +++  C++ +E T+
Sbjct: 132 LNLSVWYDCPPLEGFLGDFRFSCGSEVEIIGRANYALETKDAMYWRKNMSDCELHIEVTI 191

Query: 191 SKKSFKEGEMNRTTLVETALREGFEVQYAD--DSGCYFCKENGGACGGNATQDYYCICEN 250
           + +  KEGEMNRT +VE  ++EGFEV+Y D     C  CKE GG CGGNAT ++YCIC N
Sbjct: 192 TMEVLKEGEMNRTIVVERGIKEGFEVEYGDFYTVSCEGCKEYGGKCGGNATHEFYCICGN 251

Query: 251 GNINSFSC---------SSRIHTPASVGIAIGSINLIIFIVILSILLLVRWRKKKKRNSK 310
           G+I+ + C          SR    A +G     I L +F++ +SI         KK  S 
Sbjct: 252 GDIHPYVCKPPSSAPRGKSRTREKAIIGALGAVIVLGLFVITISIYY------TKKSMSN 311

Query: 311 KDEIEETIKRYSTQIPKRYTYSELKKITDSFKNKLGQGGFSIVYKGKLLDGRYVAVKLLN 370
           K +IEE I+RYSTQIPKRY+YS+LKKIT SFKNKLG+GGFS VYKGKL DGR VAVKLLN
Sbjct: 312 KQKIEENIRRYSTQIPKRYSYSKLKKITHSFKNKLGRGGFSTVYKGKLPDGRDVAVKLLN 371

Query: 371 ESREDAQDFINEVVSITRTSHVNIVTFLGFCYEENKRALIYEYMPKGSLDKYIFQKGLQE 430
           ESRE+ QDF+NEVVS+T+TSHVNIV+ LGFCYE   +ALIYEYMP+GSLDKYI  KGLQ 
Sbjct: 372 ESRENGQDFMNEVVSVTKTSHVNIVSLLGFCYERKYKALIYEYMPRGSLDKYISHKGLQ- 431

Query: 431 KNKAKLNWNTLYSIIVGVARGLEYLHRGCNTRILHFDIKPHNILLDNEFCPKISDFGLAK 490
           +NK +L+WNTLY I++GVARGLEYLHRGCNTRILHFDIKPHNILLDN+FCPKISDFGLAK
Sbjct: 432 RNKVELDWNTLYRIVIGVARGLEYLHRGCNTRILHFDIKPHNILLDNDFCPKISDFGLAK 491

Query: 491 QCKARESHVSMTCAKGTIGFMAPEVIIRSFGKVSHKSDVYSYGMLVLEMVGERKSPNKG- 550
           QCKA++SHVSMTC KGTIGF+APEVI R+FGKVSHKS+VYSYGMLVLE+VG RK+PN G 
Sbjct: 492 QCKAKQSHVSMTCVKGTIGFIAPEVISRNFGKVSHKSNVYSYGMLVLEIVGARKNPNDGV 551

Query: 551 -ESSEEYFPDWIYKDLAGSEIDRNCRQWGQTEEEEEMVRKMVVVSLYCIQTLPDDRPSMT 610
            + SEEYFPDWIYKD+  S+I   C  W  T E+EEM RKM++V L+CIQTLP+DRPSMT
Sbjct: 552 EQRSEEYFPDWIYKDIIQSQIVGGC-WWSNTHEDEEMARKMIIVGLHCIQTLPNDRPSMT 611

Query: 611 EVVAMLEGSADGLQIPPKPVLFESPA 623
           +VVAMLEGS D LQIPPKP LFE PA
Sbjct: 612 DVVAMLEGSIDCLQIPPKPNLFEPPA 628

BLAST of Tan0008256 vs. NCBI nr
Match: XP_011650606.2 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.4 isoform X1 [Cucumis sativus] >KGN64140.1 hypothetical protein Csa_014125 [Cucumis sativus])

HSP 1 Score: 785.8 bits (2028), Expect = 2.8e-223
Identity = 408/666 (61.26%), Postives = 492/666 (73.87%), Query Frame = 0

Query: 1   MKMSLEPNIFFFISFIAFQLSPCFSNDAGLDLFKACEMSYNCGNLVSISYPFWGNARPDF 60
           MK SL   +FF I +  F L  CFSNDA  + FKAC + Y+CG LV+ISYPFWGN R  F
Sbjct: 1   MKPSLPQFLFFTILWFDFHL--CFSNDAN-EEFKACSVYYSCGVLVNISYPFWGNERQQF 60

Query: 61  CGSSKFELKCNNNKTTTIKINSLDYYVLNINQSDQTMRMARSDLFDDYCPKSKIETAKLD 120
           CG  +FEL C +NKTTTI+INS++Y V+NINQ+D +M +ARSDL DDYCPK +I+TA L 
Sbjct: 61  CGRREFELNCKDNKTTTIQINSVEYNVVNINQTDHSMTIARSDLLDDYCPKIQIKTATLG 120

Query: 121 PSQFSYTEKDLNISIWYDCPMQEGVPEEFRFRC-SGEGEKGNTNNYALE-PEAMYLSRSI 180
            + F Y+  DLN+S+WYDCP+ EG+  E  F C S EGE+    NYALE  +AM   R++
Sbjct: 121 HNLFKYSSNDLNLSLWYDCPVLEGIRREMTFECGSSEGERRGRVNYALEKKDAMNWRRNM 180

Query: 181 RGCKVKMEATVSKKSFKEGEMNRTTLVETALREGFEVQYAD--DSGCYFCKENGGACGGN 240
             C+VKME T++K+   EGE NRT +VE  ++EGFEV+Y D     C  CKE GGACGGN
Sbjct: 181 SECRVKMEVTITKEVLTEGEKNRTMVVERGMKEGFEVEYGDLYTIACEGCKEQGGACGGN 240

Query: 241 ATQDYYCICENGNINSFSCSSRIHT------PAS---------------------VGIAI 300
            ++++ C+C NGN++ + C S   +      PA+                         I
Sbjct: 241 TSKEFRCVCGNGNVHPYICKSSSSSSPPPPPPAAPYLDVIWLDTKTGGPYTNHRRKSAII 300

Query: 301 GSINLIIFIVILSILLLVRWRKKKKRNSKKDEIEETIKRYSTQIPKRYTYSELKKITDSF 360
           G  +   F++++SI++ + W  K+KR S KD+IE+ I+RYS Q PKRY+YS+LKKITD F
Sbjct: 301 GGTSSGGFVLVISIIIFIYW--KRKRTSNKDKIEKIIRRYSIQTPKRYSYSKLKKITDCF 360

Query: 361 KNKLGQGGFSIVYKGKLLDGRYVAVKLLNESR-EDAQDFINEVVSITRTSHVNIVTFLGF 420
            NKLGQGGFS VYKGKL +G  VAVKLLNESR E+ QDFINEVVSI +TSH+NIVT +GF
Sbjct: 361 NNKLGQGGFSTVYKGKLPNGCDVAVKLLNESRQENGQDFINEVVSIAKTSHINIVTLIGF 420

Query: 421 CYEENKRALIYEYMPKGSLDKYIFQKGLQEKNKAKLNWNTLYSIIVGVARGLEYLHRGCN 480
           CYE+NKRALIYEYMPKGSLDKYI+   LQE N  KL+WNTLY+I++GVARGLEYLHRGCN
Sbjct: 421 CYEQNKRALIYEYMPKGSLDKYIYHNRLQE-NDMKLDWNTLYNIVIGVARGLEYLHRGCN 480

Query: 481 TRILHFDIKPHNILLDNEFCPKISDFGLAKQCKARESHVSMTCAKGTIGFMAPEVIIRSF 540
           TRILHFDIKPHNILLD++FCPKISDFGLAKQC+ARESHVSMT  KGTIGF+APEVI R+ 
Sbjct: 481 TRILHFDIKPHNILLDSDFCPKISDFGLAKQCEARESHVSMTGVKGTIGFIAPEVIFRNS 540

Query: 541 GKVSHKSDVYSYGMLVLEMVGERKSPNKG--ESSEEYFPDWIYKDLAGSEIDRNCRQWGQ 600
           GKVSHKSDVYSYGML+LEMVG RK PN+G  + SE YFPDWIYKDL  SEID  C  WG 
Sbjct: 541 GKVSHKSDVYSYGMLILEMVGARKKPNEGVEQKSEAYFPDWIYKDLTQSEIDGGC-WWGN 600

Query: 601 TEEEEEMVRKMVVVSLYCIQTLPDDRPSMTEVVAMLEGSADGLQIPPKPVLF-----ESP 628
           T+EEEEM RKM++V L+CIQTLPDDRPSMT VV MLEGS D LQIPPKP ++     E P
Sbjct: 601 TKEEEEMARKMIIVGLHCIQTLPDDRPSMTNVVVMLEGSVDVLQIPPKPNMYGPPNIEQP 659

BLAST of Tan0008256 vs. NCBI nr
Match: XP_022923743.1 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.4 isoform X3 [Cucurbita moschata])

HSP 1 Score: 776.2 bits (2003), Expect = 2.2e-220
Identity = 387/641 (60.37%), Postives = 480/641 (74.88%), Query Frame = 0

Query: 1   MKMSLEPNIFFFISFIAFQLSPCFSNDAGLDLFKACEMSYNCGNLVSISYPFWGNARPDF 60
           M  SL   +FFF + + + L  CF  D  +D FKAC++ YNCG+ V+I+YPFWGN RP F
Sbjct: 24  MTPSLPLFLFFFFTIVWYDLPLCF-GDGTVDEFKACDVYYNCGDFVNITYPFWGNERPVF 83

Query: 61  CGSSKFELKCNNNKTTTIKINSLDYYVLNINQSDQTMRMARSDLFDDYCPKSKIETAKLD 120
           CG  +FEL C +N+TTT++I+S++++VLNI++S  TM +ARSDL  DYCPK + +T  +D
Sbjct: 84  CGRREFELNCKDNRTTTMEISSIEFHVLNISRSKHTMTIARSDLRTDYCPKIETKTTTID 143

Query: 121 PSQFSYTEKDLNISIWYDCPMQEGVPEEFRFRCSGEGEKGNTNNYALEPEAMYLSRSIRG 180
              F YT  DLN+S+WYDCP+Q G+ + +RF C  EGE     NYA E EA+  SR++  
Sbjct: 144 YRLFKYTLNDLNLSVWYDCPLQPGILDNYRFTCGSEGEIRGRANYAFETEALNRSRNMSE 203

Query: 181 CKVKMEATVSKKSFKEGEMNRTTLVETALREGFEVQYAD--DSGCYFCKENGGACGGNAT 240
           C++ +E T++K+ F+E   NRT  VE  ++ GF+V+Y D     C  CK  GG CGGNAT
Sbjct: 204 CRLNIEVTITKEVFEETHKNRTMAVEQGVKRGFDVEYGDFYTVACEGCKVYGGKCGGNAT 263

Query: 241 QDYYCICENGNINSFSCSSRIHTPASVGIAIGSINLIIFIVILSILLLVRWRKKKKRNSK 300
            ++YCIC +G+I+ + C S    P       G+  +I++I+I+  +       K++  S 
Sbjct: 264 HEFYCICSDGDIHPYVCKS---PPPP-----GATTIIVYIIIIISIYY-----KRRSISN 323

Query: 301 KDEIEETIKRYSTQIPKRYTYSELKKITDSFKNKLGQGGFSIVYKGKLLDGRYVAVKLLN 360
            D+IEE I+ YSTQ PKRYTYS+LKKIT SF NK+GQGGFS VYKGKL DGR VAVKLLN
Sbjct: 324 NDKIEEIIRNYSTQTPKRYTYSKLKKITGSFNNKIGQGGFSSVYKGKLPDGRDVAVKLLN 383

Query: 361 ESREDAQDFINEVVSITRTSHVNIVTFLGFCYEENKRALIYEYMPKGSLDKYIFQKGLQE 420
           ES+ + ++F+NEVVS  +TSHVNI T LGFCYE NKRALIY+YM KGSLDKYI    +QE
Sbjct: 384 ESKSNGEEFMNEVVSFAKTSHVNIATLLGFCYERNKRALIYDYMAKGSLDKYISSNRIQE 443

Query: 421 KNKAKLNWNTLYSIIVGVARGLEYLHRGCNTRILHFDIKPHNILLDNEFCPKISDFGLAK 480
           K + KL+WNTLY+I++GVARGLEYLHRGCNTRILHFDIKPHNILLD++FCPKI+DFGLAK
Sbjct: 444 KGE-KLDWNTLYNIVIGVARGLEYLHRGCNTRILHFDIKPHNILLDDDFCPKITDFGLAK 503

Query: 481 QCKARESHVSMTCAKGTIGFMAPEVIIRSFGKVSHKSDVYSYGMLVLEMVGERKSPNKG- 540
           QC+A+ESHVSMTC KGTIGF+APE+I R+ GKVSHKSDVYSYGMLVLEMVGERKSPN+G 
Sbjct: 504 QCRAKESHVSMTCVKGTIGFIAPEIIFRNLGKVSHKSDVYSYGMLVLEMVGERKSPNQGV 563

Query: 541 -ESSEEYFPDWIYKDLAGSEIDRNCRQWGQTEEEEEMVRKMVVVSLYCIQTLPDDRPSMT 600
            +SS+EYFPDWIYKDL  SEI   C  WG TEEEEEM RKM++V L CIQTLP DRPSMT
Sbjct: 564 EQSSDEYFPDWIYKDLTQSEIHGGC-WWGNTEEEEEMARKMIIVGLCCIQTLPGDRPSMT 623

Query: 601 EVVAMLEGSADGLQIPPKPVLFESPAASL------SSSISY 632
           + V+MLEGS DGLQIPPKP LF  PAA L      SSS SY
Sbjct: 624 DAVSMLEGSVDGLQIPPKPDLFGPPAADLLQVAASSSSTSY 648

BLAST of Tan0008256 vs. ExPASy TrEMBL
Match: A0A6J1C9Y1 (uncharacterized protein LOC111008790 OS=Momordica charantia OX=3673 GN=LOC111008790 PE=4 SV=1)

HSP 1 Score: 794.3 bits (2050), Expect = 3.8e-226
Identity = 402/632 (63.61%), Postives = 478/632 (75.63%), Query Frame = 0

Query: 9   IFFFISFI---AFQLSPCFSNDAGLDLFKACEMSYNCGNLVSISYPFWGNARPDFCGSSK 68
           +FFF++F     +   P  S +  L  FKAC + YNCGNLV+ISYPFWGN R DFCG  +
Sbjct: 7   LFFFVAFFFSSVWSDLPLCSGEDALSEFKACGVDYNCGNLVNISYPFWGNERQDFCGRRE 66

Query: 69  FELKCNNNKTTTIKINSLDYYVLNINQSDQTMRMARSDLFDDYCPKSKIETAKLDPSQFS 128
           F L C  N TTTI+INSL+Y VL+INQ+D  M +ARSDLFDDYCPK++IETAK D    +
Sbjct: 67  FVLNCKQNLTTTIQINSLEYKVLHINQTDHRMTIARSDLFDDYCPKNQIETAKFDHHLLT 126

Query: 129 YTEKDLNISIWYDCPMQEGVPEEFRFRCSGEGEKGNTNNYALEPEAMYLSRSIRGCKVKM 188
           Y   D N+S WYDCP Q+G+P+EFRF C  EGE+    NYALEPE M LS +IRGCK+ +
Sbjct: 127 YYWYDRNMSFWYDCPPQQGIPDEFRFFCGWEGERSGRANYALEPEEMNLSGNIRGCKMNV 186

Query: 189 EATVSKKSFKEGEMNRTTLVETALREGFEVQYAD--DSGCYFCKENGGACGGNATQDYYC 248
           E  V+ +  +EG+ NRT  +E AL+ GF+V+Y D     C  CK++GG CG N T  +YC
Sbjct: 187 EVXVTIEGLEEGKENRTARLEKALQRGFDVEYGDLHTMACDVCKQSGGYCGANGTYPFYC 246

Query: 249 IC-ENGNINSFSCSSRIHTPAS--------VGIAIGSINLIIFIVILSILLLVRWRKKKK 308
           IC + G+ + + C+S   +  S        V +A GS   IIF+++  I +       + 
Sbjct: 247 ICGDRGDAHPYVCTSISTSRKSSFQTKLVIVAVASGS---IIFLILFMIFIYY----TRI 306

Query: 309 RNSKKDEIEETIKRYSTQIPKRYTYSELKKITDSFKNKLGQGGFSIVYKGKLLDGRYVAV 368
           R S K+EIEE ++R+ST IPKRY+YS+LKKIT SFKNKLGQGGFS VYKGKL DGR VAV
Sbjct: 307 RISNKEEIEEIVRRHSTHIPKRYSYSKLKKITHSFKNKLGQGGFSTVYKGKLPDGRDVAV 366

Query: 369 KLLNESREDAQDFINEVVSITRTSHVNIVTFLGFCYEENKRALIYEYMPKGSLDKYIFQK 428
           KLLNES+E+ QDFINEVVSIT TSHVNI T LGFCYE N+RAL+YEYMPKGSLDKYIF K
Sbjct: 367 KLLNESKENGQDFINEVVSITTTSHVNIATLLGFCYERNRRALVYEYMPKGSLDKYIFHK 426

Query: 429 GLQEKNKAKLNWNTLYSIIVGVARGLEYLHRGCNTRILHFDIKPHNILLDNEFCPKISDF 488
             Q K+   L+WNTLY+I++GVARGLEYLHRGCNTRILHFDIKPHNILLDNEFCPKISDF
Sbjct: 427 DPQ-KSDMGLDWNTLYTIVIGVARGLEYLHRGCNTRILHFDIKPHNILLDNEFCPKISDF 486

Query: 489 GLAKQCKARESHVSMTCAKGTIGFMAPEVIIRSFGKVSHKSDVYSYGMLVLEMVGERKSP 548
           GLAKQC+ARESHVSMT  KGTIGFMAPEV+ R+ GKVSHKSDVYSYGMLVLEMVGERK+P
Sbjct: 487 GLAKQCRARESHVSMTGVKGTIGFMAPEVVFRNIGKVSHKSDVYSYGMLVLEMVGERKNP 546

Query: 549 NK--GESSEEYFPDWIYKDLAGSEIDRNCRQWGQTEEEEEMVRKMVVVSLYCIQTLPDDR 608
           N+  G+ SEEYFPDWIYKDL  SE D  C  WG TEEE+EM RKM++V L+CIQTLPDDR
Sbjct: 547 NEGVGQRSEEYFPDWIYKDLTQSETDEGC-SWGNTEEEDEMARKMIIVGLHCIQTLPDDR 606

Query: 609 PSMTEVVAMLEGSADGLQIPPKPVLFESPAAS 625
           PSM++VV MLEGS DGLQIPPKP    +P  +
Sbjct: 607 PSMSDVVGMLEGSVDGLQIPPKPNFMGTPTTT 629

BLAST of Tan0008256 vs. ExPASy TrEMBL
Match: A0A0A0LQF0 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G042510 PE=4 SV=1)

HSP 1 Score: 785.8 bits (2028), Expect = 1.3e-223
Identity = 408/666 (61.26%), Postives = 492/666 (73.87%), Query Frame = 0

Query: 1   MKMSLEPNIFFFISFIAFQLSPCFSNDAGLDLFKACEMSYNCGNLVSISYPFWGNARPDF 60
           MK SL   +FF I +  F L  CFSNDA  + FKAC + Y+CG LV+ISYPFWGN R  F
Sbjct: 1   MKPSLPQFLFFTILWFDFHL--CFSNDAN-EEFKACSVYYSCGVLVNISYPFWGNERQQF 60

Query: 61  CGSSKFELKCNNNKTTTIKINSLDYYVLNINQSDQTMRMARSDLFDDYCPKSKIETAKLD 120
           CG  +FEL C +NKTTTI+INS++Y V+NINQ+D +M +ARSDL DDYCPK +I+TA L 
Sbjct: 61  CGRREFELNCKDNKTTTIQINSVEYNVVNINQTDHSMTIARSDLLDDYCPKIQIKTATLG 120

Query: 121 PSQFSYTEKDLNISIWYDCPMQEGVPEEFRFRC-SGEGEKGNTNNYALE-PEAMYLSRSI 180
            + F Y+  DLN+S+WYDCP+ EG+  E  F C S EGE+    NYALE  +AM   R++
Sbjct: 121 HNLFKYSSNDLNLSLWYDCPVLEGIRREMTFECGSSEGERRGRVNYALEKKDAMNWRRNM 180

Query: 181 RGCKVKMEATVSKKSFKEGEMNRTTLVETALREGFEVQYAD--DSGCYFCKENGGACGGN 240
             C+VKME T++K+   EGE NRT +VE  ++EGFEV+Y D     C  CKE GGACGGN
Sbjct: 181 SECRVKMEVTITKEVLTEGEKNRTMVVERGMKEGFEVEYGDLYTIACEGCKEQGGACGGN 240

Query: 241 ATQDYYCICENGNINSFSCSSRIHT------PAS---------------------VGIAI 300
            ++++ C+C NGN++ + C S   +      PA+                         I
Sbjct: 241 TSKEFRCVCGNGNVHPYICKSSSSSSPPPPPPAAPYLDVIWLDTKTGGPYTNHRRKSAII 300

Query: 301 GSINLIIFIVILSILLLVRWRKKKKRNSKKDEIEETIKRYSTQIPKRYTYSELKKITDSF 360
           G  +   F++++SI++ + W  K+KR S KD+IE+ I+RYS Q PKRY+YS+LKKITD F
Sbjct: 301 GGTSSGGFVLVISIIIFIYW--KRKRTSNKDKIEKIIRRYSIQTPKRYSYSKLKKITDCF 360

Query: 361 KNKLGQGGFSIVYKGKLLDGRYVAVKLLNESR-EDAQDFINEVVSITRTSHVNIVTFLGF 420
            NKLGQGGFS VYKGKL +G  VAVKLLNESR E+ QDFINEVVSI +TSH+NIVT +GF
Sbjct: 361 NNKLGQGGFSTVYKGKLPNGCDVAVKLLNESRQENGQDFINEVVSIAKTSHINIVTLIGF 420

Query: 421 CYEENKRALIYEYMPKGSLDKYIFQKGLQEKNKAKLNWNTLYSIIVGVARGLEYLHRGCN 480
           CYE+NKRALIYEYMPKGSLDKYI+   LQE N  KL+WNTLY+I++GVARGLEYLHRGCN
Sbjct: 421 CYEQNKRALIYEYMPKGSLDKYIYHNRLQE-NDMKLDWNTLYNIVIGVARGLEYLHRGCN 480

Query: 481 TRILHFDIKPHNILLDNEFCPKISDFGLAKQCKARESHVSMTCAKGTIGFMAPEVIIRSF 540
           TRILHFDIKPHNILLD++FCPKISDFGLAKQC+ARESHVSMT  KGTIGF+APEVI R+ 
Sbjct: 481 TRILHFDIKPHNILLDSDFCPKISDFGLAKQCEARESHVSMTGVKGTIGFIAPEVIFRNS 540

Query: 541 GKVSHKSDVYSYGMLVLEMVGERKSPNKG--ESSEEYFPDWIYKDLAGSEIDRNCRQWGQ 600
           GKVSHKSDVYSYGML+LEMVG RK PN+G  + SE YFPDWIYKDL  SEID  C  WG 
Sbjct: 541 GKVSHKSDVYSYGMLILEMVGARKKPNEGVEQKSEAYFPDWIYKDLTQSEIDGGC-WWGN 600

Query: 601 TEEEEEMVRKMVVVSLYCIQTLPDDRPSMTEVVAMLEGSADGLQIPPKPVLF-----ESP 628
           T+EEEEM RKM++V L+CIQTLPDDRPSMT VV MLEGS D LQIPPKP ++     E P
Sbjct: 601 TKEEEEMARKMIIVGLHCIQTLPDDRPSMTNVVVMLEGSVDVLQIPPKPNMYGPPNIEQP 659

BLAST of Tan0008256 vs. ExPASy TrEMBL
Match: A0A6J1EAG8 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.4 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111431357 PE=4 SV=1)

HSP 1 Score: 776.2 bits (2003), Expect = 1.1e-220
Identity = 387/641 (60.37%), Postives = 480/641 (74.88%), Query Frame = 0

Query: 1   MKMSLEPNIFFFISFIAFQLSPCFSNDAGLDLFKACEMSYNCGNLVSISYPFWGNARPDF 60
           M  SL   +FFF + + + L  CF  D  +D FKAC++ YNCG+ V+I+YPFWGN RP F
Sbjct: 24  MTPSLPLFLFFFFTIVWYDLPLCF-GDGTVDEFKACDVYYNCGDFVNITYPFWGNERPVF 83

Query: 61  CGSSKFELKCNNNKTTTIKINSLDYYVLNINQSDQTMRMARSDLFDDYCPKSKIETAKLD 120
           CG  +FEL C +N+TTT++I+S++++VLNI++S  TM +ARSDL  DYCPK + +T  +D
Sbjct: 84  CGRREFELNCKDNRTTTMEISSIEFHVLNISRSKHTMTIARSDLRTDYCPKIETKTTTID 143

Query: 121 PSQFSYTEKDLNISIWYDCPMQEGVPEEFRFRCSGEGEKGNTNNYALEPEAMYLSRSIRG 180
              F YT  DLN+S+WYDCP+Q G+ + +RF C  EGE     NYA E EA+  SR++  
Sbjct: 144 YRLFKYTLNDLNLSVWYDCPLQPGILDNYRFTCGSEGEIRGRANYAFETEALNRSRNMSE 203

Query: 181 CKVKMEATVSKKSFKEGEMNRTTLVETALREGFEVQYAD--DSGCYFCKENGGACGGNAT 240
           C++ +E T++K+ F+E   NRT  VE  ++ GF+V+Y D     C  CK  GG CGGNAT
Sbjct: 204 CRLNIEVTITKEVFEETHKNRTMAVEQGVKRGFDVEYGDFYTVACEGCKVYGGKCGGNAT 263

Query: 241 QDYYCICENGNINSFSCSSRIHTPASVGIAIGSINLIIFIVILSILLLVRWRKKKKRNSK 300
            ++YCIC +G+I+ + C S    P       G+  +I++I+I+  +       K++  S 
Sbjct: 264 HEFYCICSDGDIHPYVCKS---PPPP-----GATTIIVYIIIIISIYY-----KRRSISN 323

Query: 301 KDEIEETIKRYSTQIPKRYTYSELKKITDSFKNKLGQGGFSIVYKGKLLDGRYVAVKLLN 360
            D+IEE I+ YSTQ PKRYTYS+LKKIT SF NK+GQGGFS VYKGKL DGR VAVKLLN
Sbjct: 324 NDKIEEIIRNYSTQTPKRYTYSKLKKITGSFNNKIGQGGFSSVYKGKLPDGRDVAVKLLN 383

Query: 361 ESREDAQDFINEVVSITRTSHVNIVTFLGFCYEENKRALIYEYMPKGSLDKYIFQKGLQE 420
           ES+ + ++F+NEVVS  +TSHVNI T LGFCYE NKRALIY+YM KGSLDKYI    +QE
Sbjct: 384 ESKSNGEEFMNEVVSFAKTSHVNIATLLGFCYERNKRALIYDYMAKGSLDKYISSNRIQE 443

Query: 421 KNKAKLNWNTLYSIIVGVARGLEYLHRGCNTRILHFDIKPHNILLDNEFCPKISDFGLAK 480
           K + KL+WNTLY+I++GVARGLEYLHRGCNTRILHFDIKPHNILLD++FCPKI+DFGLAK
Sbjct: 444 KGE-KLDWNTLYNIVIGVARGLEYLHRGCNTRILHFDIKPHNILLDDDFCPKITDFGLAK 503

Query: 481 QCKARESHVSMTCAKGTIGFMAPEVIIRSFGKVSHKSDVYSYGMLVLEMVGERKSPNKG- 540
           QC+A+ESHVSMTC KGTIGF+APE+I R+ GKVSHKSDVYSYGMLVLEMVGERKSPN+G 
Sbjct: 504 QCRAKESHVSMTCVKGTIGFIAPEIIFRNLGKVSHKSDVYSYGMLVLEMVGERKSPNQGV 563

Query: 541 -ESSEEYFPDWIYKDLAGSEIDRNCRQWGQTEEEEEMVRKMVVVSLYCIQTLPDDRPSMT 600
            +SS+EYFPDWIYKDL  SEI   C  WG TEEEEEM RKM++V L CIQTLP DRPSMT
Sbjct: 564 EQSSDEYFPDWIYKDLTQSEIHGGC-WWGNTEEEEEMARKMIIVGLCCIQTLPGDRPSMT 623

Query: 601 EVVAMLEGSADGLQIPPKPVLFESPAASL------SSSISY 632
           + V+MLEGS DGLQIPPKP LF  PAA L      SSS SY
Sbjct: 624 DAVSMLEGSVDGLQIPPKPDLFGPPAADLLQVAASSSSTSY 648

BLAST of Tan0008256 vs. ExPASy TrEMBL
Match: A0A6J1E6Z8 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.4 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111431357 PE=4 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 1.8e-220
Identity = 387/646 (59.91%), Postives = 481/646 (74.46%), Query Frame = 0

Query: 1   MKMSLEPNIFFFISFIAFQLSPCFSNDAGLDLFKACEMSYNCGNLVSISYPFWGNARPDF 60
           M  SL   +FFF + + + L  CF  D  +D FKAC++ YNCG+ V+I+YPFWGN RP F
Sbjct: 24  MTPSLPLFLFFFFTIVWYDLPLCF-GDGTVDEFKACDVYYNCGDFVNITYPFWGNERPVF 83

Query: 61  CGSSKFELKCNNNKTTTIKINSLDYYVLNINQSDQTMRMARSDLFDDYCPKSKIETAKLD 120
           CG  +FEL C +N+TTT++I+S++++VLNI++S  TM +ARSDL  DYCPK + +T  +D
Sbjct: 84  CGRREFELNCKDNRTTTMEISSIEFHVLNISRSKHTMTIARSDLRTDYCPKIETKTTTID 143

Query: 121 PSQFSYTEKDLNISIWYDCPMQEGVPEEFRFRCSGEGEKGNTNNYALEPEAMYLSRSIRG 180
              F YT  DLN+S+WYDCP+Q G+ + +RF C  EGE     NYA E EA+  SR++  
Sbjct: 144 YRLFKYTLNDLNLSVWYDCPLQPGILDNYRFTCGSEGEIRGRANYAFETEALNRSRNMSE 203

Query: 181 CKVKMEATVSKKSFKEGEMNRTTLVETALREGFEVQYAD--DSGCYFCKENGGACGGNAT 240
           C++ +E T++K+ F+E   NRT  VE  ++ GF+V+Y D     C  CK  GG CGGNAT
Sbjct: 204 CRLNIEVTITKEVFEETHKNRTMAVEQGVKRGFDVEYGDFYTVACEGCKVYGGKCGGNAT 263

Query: 241 QDYYCICENGNINSFSCSS-----RIHTPASVGIAIGSINLIIFIVILSILLLVRWRKKK 300
            ++YCIC +G+I+ + C S        +       IG+  +I++I+I+  +       K+
Sbjct: 264 HEFYCICSDGDIHPYVCKSPPPPDLDQSDTGPKDIIGATTIIVYIIIIISIYY-----KR 323

Query: 301 KRNSKKDEIEETIKRYSTQIPKRYTYSELKKITDSFKNKLGQGGFSIVYKGKLLDGRYVA 360
           +  S  D+IEE I+ YSTQ PKRYTYS+LKKIT SF NK+GQGGFS VYKGKL DGR VA
Sbjct: 324 RSISNNDKIEEIIRNYSTQTPKRYTYSKLKKITGSFNNKIGQGGFSSVYKGKLPDGRDVA 383

Query: 361 VKLLNESREDAQDFINEVVSITRTSHVNIVTFLGFCYEENKRALIYEYMPKGSLDKYIFQ 420
           VKLLNES+ + ++F+NEVVS  +TSHVNI T LGFCYE NKRALIY+YM KGSLDKYI  
Sbjct: 384 VKLLNESKSNGEEFMNEVVSFAKTSHVNIATLLGFCYERNKRALIYDYMAKGSLDKYISS 443

Query: 421 KGLQEKNKAKLNWNTLYSIIVGVARGLEYLHRGCNTRILHFDIKPHNILLDNEFCPKISD 480
             +QEK + KL+WNTLY+I++GVARGLEYLHRGCNTRILHFDIKPHNILLD++FCPKI+D
Sbjct: 444 NRIQEKGE-KLDWNTLYNIVIGVARGLEYLHRGCNTRILHFDIKPHNILLDDDFCPKITD 503

Query: 481 FGLAKQCKARESHVSMTCAKGTIGFMAPEVIIRSFGKVSHKSDVYSYGMLVLEMVGERKS 540
           FGLAKQC+A+ESHVSMTC KGTIGF+APE+I R+ GKVSHKSDVYSYGMLVLEMVGERKS
Sbjct: 504 FGLAKQCRAKESHVSMTCVKGTIGFIAPEIIFRNLGKVSHKSDVYSYGMLVLEMVGERKS 563

Query: 541 PNKG--ESSEEYFPDWIYKDLAGSEIDRNCRQWGQTEEEEEMVRKMVVVSLYCIQTLPDD 600
           PN+G  +SS+EYFPDWIYKDL  SEI   C  WG TEEEEEM RKM++V L CIQTLP D
Sbjct: 564 PNQGVEQSSDEYFPDWIYKDLTQSEIHGGC-WWGNTEEEEEMARKMIIVGLCCIQTLPGD 623

Query: 601 RPSMTEVVAMLEGSADGLQIPPKPVLFESPAASL------SSSISY 632
           RPSMT+ V+MLEGS DGLQIPPKP LF  PAA L      SSS SY
Sbjct: 624 RPSMTDAVSMLEGSVDGLQIPPKPDLFGPPAADLLQVAASSSSTSY 661

BLAST of Tan0008256 vs. ExPASy TrEMBL
Match: A0A5D3BIU5 (LEAF RUST 10 DISEASE-RESISTANCE LOCUS RECEPTOR-LIKE PROTEIN KINASE-like 2.4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002100 PE=4 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 3.1e-220
Identity = 395/643 (61.43%), Postives = 483/643 (75.12%), Query Frame = 0

Query: 1   MKMSLEPNIFFFISFIAFQLSPCFSNDAGLDLFKACEMSYNCGNLVSISYPFWGNARPDF 60
           MK+SL   +F F + I F L  C+++D+  D FKAC + YNCG+LV+ISYPFWGN R  F
Sbjct: 1   MKISLP--LFLFFTIILFDLHLCYADDSH-DEFKACGVYYNCGDLVNISYPFWGNERQSF 60

Query: 61  CGSSKFELKCNNNKTTTIKINSLDYYVLNINQSDQTMRMARSDLFDDYCPKSKIETAKLD 120
           CG  +F L C +N+ TTI+I    Y V+NI+QSD  M +ARS+LFDDYCP ++I  A LD
Sbjct: 61  CGRREFGLICKDNEKTTIQIRYRQYIVVNISQSDHRMTIARSELFDDYCPNNEIGVATLD 120

Query: 121 PSQFSYTEKDLNISIWYDCPMQEGVPEEFRFRCSGEGEKGNTNNYALEP-EAMYLSRSIR 180
            S F Y+  DLN+S+WYDCP+  G+P+ F F+C  EGE+    NYALE  EAM  S    
Sbjct: 121 FSLFKYSSNDLNLSVWYDCPVLPGIPQNFEFKCGSEGERSGKANYALEENEAMSWSAYTG 180

Query: 181 GCKVKMEATVSKKSFKEGEMNRTTLVETALREGFEVQYAD--DSGCYFCKENGGACGGNA 240
            C++K+E T++ + F+EG+MNRT ++E  ++EGFEV+Y D     C  CKE GGACG NA
Sbjct: 181 ECRIKIEVTIALEVFREGKMNRTMMMERGMKEGFEVEYGDLYTIACEGCKEYGGACGRNA 240

Query: 241 TQDYYCICENGNINSFSC-----SSRIHTPASVGIAIGSINLIIFIVILSILLLVRWRKK 300
           T+++ CIC NG+++ + C       R+     +    G+  LIIFI+I+ I         
Sbjct: 241 TKEFLCICRNGDVHPYVCKPPPGKRRMSVKVIIAAFSGTSGLIIFIIIIGIYY------T 300

Query: 301 KKRNSKKDEIEETIKRYSTQIPKRYTYSELKKITDSFKNKLGQGGFSIVYKGKLLDGRYV 360
           +K  S KD+IEE+I+RYST +PKRY+YS+LKKIT+SFKNKLGQGGFS VYKGKL DGR V
Sbjct: 301 RKNKSNKDKIEESIRRYSTLMPKRYSYSKLKKITNSFKNKLGQGGFSTVYKGKLPDGRDV 360

Query: 361 AVKLLNESREDAQDFINEVVSITRTSHVNIVTFLGFCYEENKRALIYEYMPKGSLDKYIF 420
           AVKLLNESRE+ QDF+NEVVSITRTSHVNI + LGFCYE  KRALIYEYMP+GSLD+YI 
Sbjct: 361 AVKLLNESRENGQDFMNEVVSITRTSHVNIASLLGFCYERKKRALIYEYMPRGSLDQYIS 420

Query: 421 QKGLQEKNKAKLNWNTLYSIIVGVARGLEYLHRGCNTRILHFDIKPHNILLDNEFCPKIS 480
            KG   +N  +L+WNTLYSI++GVARGLEYLHRGC TRILHFDIKPHNILLD++FCPKIS
Sbjct: 421 HKG-PHRNNVELDWNTLYSIVIGVARGLEYLHRGCITRILHFDIKPHNILLDSDFCPKIS 480

Query: 481 DFGLAKQCKARESHVSMTCAKGTIGFMAPEVIIRSFGKVSHKSDVYSYGMLVLEMVGERK 540
           DFGLAKQC+A+ESHV+MT  KGT+GF+APEVI R+FGKVSHKSDVYSYGMLVLEMVG RK
Sbjct: 481 DFGLAKQCRAKESHVTMTGVKGTVGFIAPEVIFRNFGKVSHKSDVYSYGMLVLEMVGARK 540

Query: 541 SPNK--GESSEEYFPDWIYKDLAGSEIDRNCRQWGQTEEEEEMVRKMVVVSLYCIQTLPD 600
           +PN   G+ S EYFPDWIY +L  SEID    + G TEEE+EM RKM++V L+CIQTLPD
Sbjct: 541 NPNDGVGQGSGEYFPDWIYNNLTQSEIDEGFLR-GNTEEEKEMTRKMIIVGLHCIQTLPD 600

Query: 601 DRPSMTEVVAMLEGSADGLQIPPKPVL-----FESPAASLSSS 629
           DRPSMT+VVAMLEGS DGLQIP KP L     FE P  S SSS
Sbjct: 601 DRPSMTDVVAMLEGSVDGLQIPSKPNLFGPPTFEHPQPSSSSS 632

BLAST of Tan0008256 vs. TAIR 10
Match: AT1G66930.1 (Protein kinase superfamily protein )

HSP 1 Score: 416.8 bits (1070), Expect = 3.2e-116
Identity = 245/606 (40.43%), Postives = 354/606 (58.42%), Query Frame = 0

Query: 31  DLFKACEMSYNCGNLVSISYPFWGNARPDFCGSSKFELKCNNNKTTTIKINSLDYYVLNI 90
           +L + C   ++CGN  ++ YPFW   R ++CG   F+L C+      + I S+++ +LN+
Sbjct: 66  ELRRRCSERFSCGNQRNLYYPFWIPGR-EYCGHPDFKLDCSGG-FAELNIASVNFRILNM 125

Query: 91  NQSDQTMRMARSDLFDDYCPKSKIETAKLDPSQFSYTEKDLNISIWYDCPMQEGVPEEFR 150
           +      R+ARSD  +D CP + +    L  +   ++     ++++YDC +       F 
Sbjct: 126 SYDSSNKRLARSDYLNDLCPPNPLNDEPLIETVLQFSADTELLTLYYDCQLNSSATSPFP 185

Query: 151 FRCSGE-GEKGNTNNYALEPEAMYLSRSIRG--------CKVKMEATVSKKSFKEGEMN- 210
               GE G     + Y     +  L    RG        CK K+    S  +    + N 
Sbjct: 186 SSYFGELGCDEGRSYYVTRNLSSPLLDRFRGVLNNLREMCKRKVSVPASGPALNTLQTNP 245

Query: 211 RTTLVETALREGFEVQYADDSGCYFCKENGGACGGNATQDYYCICENGNINSFSCSSRIH 270
            +  ++ AL  GFE+Q  D+S C  C  +GG+CG N       I                
Sbjct: 246 NSNNLKMALEHGFELQ--DNSNCSMCVLSGGSCGYNQNSMAIII---------------- 305

Query: 271 TPASVGIAIGSI-NLIIFIVILSILLLVR-WRKKKKRNSKKDEIEETIKRYSTQIP-KRY 330
               + +A+ +I   I F+V+L     VR +R +K  + ++ E      +    IP K Y
Sbjct: 306 ---GIFVALCTIGGFIAFLVLLCPCCKVRIFRNRKTSDDRRQE------KLKALIPLKHY 365

Query: 331 TYSELKKITDSFKNKLGQGGFSIVYKGKLLDGRYVAVKLLNESR-EDAQDFINEVVSITR 390
           TY+++K++T SF   +G+GGF IVY+G L DGR VAVK+L ES+  +++DFINEV S+++
Sbjct: 366 TYAQVKRMTKSFAEVVGRGGFGIVYRGTLCDGRMVAVKVLKESKGNNSEDFINEVSSMSQ 425

Query: 391 TSHVNIVTFLGFCYEENKRALIYEYMPKGSLDKYIFQKGLQEKNKAKLNWNTLYSIIVGV 450
           TSHVNIV+ LGFC E ++RA+IYE++  GSLDK+I      EK    L+   LY I +GV
Sbjct: 426 TSHVNIVSLLGFCSEGSRRAIIYEFLENGSLDKFI-----SEKTSVILDLTALYGIALGV 485

Query: 451 ARGLEYLHRGCNTRILHFDIKPHNILLDNEFCPKISDFGLAKQCKARESHVSMTCAKGTI 510
           ARGLEYLH GC TRI+HFDIKP N+LLD+   PK+SDFGLAK C+ +ES +S+   +GTI
Sbjct: 486 ARGLEYLHYGCKTRIVHFDIKPQNVLLDDNLSPKVSDFGLAKLCEKKESVMSLMDTRGTI 545

Query: 511 GFMAPEVIIRSFGKVSHKSDVYSYGMLVLEMVGERKSPNKGESSEE----YFPDWIYKDL 570
           G++APE+I R +G VSHKSDVYSYGMLV EM+G RK    G++S      YFP+WIYKDL
Sbjct: 546 GYIAPEMISRVYGSVSHKSDVYSYGMLVFEMIGARKKERFGQNSANGSSMYFPEWIYKDL 605

Query: 571 AGSEI-DRNCRQWGQTEEEEEMVRKMVVVSLYCIQTLPDDRPSMTEVVAMLEGSADGLQI 618
             ++  D    + G + EEEE+ +KM +V L+CIQ+ P DRP M +VV M+EGS D L++
Sbjct: 606 EKADNGDLEHIEIGISSEEEEIAKKMTLVGLWCIQSSPSDRPPMNKVVEMMEGSLDALEV 637

BLAST of Tan0008256 vs. TAIR 10
Match: AT1G66920.2 (Protein kinase superfamily protein )

HSP 1 Score: 415.2 bits (1066), Expect = 9.2e-116
Identity = 252/630 (40.00%), Postives = 362/630 (57.46%), Query Frame = 0

Query: 9   IFFFISFIAFQLSPCFSNDAGLDLFKACEMSYNCGNLVSISYPFWGNARPDFCGSSKFEL 68
           +  F+ F  F   PC S+   L     CE  + CGN ++  +PFWG  RP+ CG    EL
Sbjct: 9   LVLFLFFSLFYHLPCASSKQTLGW---CESQFQCGN-ITAGFPFWGGNRPEVCGHPLLEL 68

Query: 69  KCNNNKTTTIKINSLDYYVLNINQSDQTMRMARSDLFDDYC-PKSKIETAKLDPSQFSYT 128
            C +N  T++ I+   Y+VL+IN +  T+R+AR+D     C        A L P  F   
Sbjct: 69  HCLDN-ITSLTISDHLYHVLSINHTYNTLRVARTDFLQSICLSPFPFANATLPPEIFDIL 128

Query: 129 EKDLNISIWYDCPMQEGVPEEFRFRCSGEGEKGNTNNYALEPEAMYLSRSIRGCKVKMEA 188
               +++++   P+   +P+  R+ C   G    ++N         L   +  C+ +   
Sbjct: 129 PTYKSVTLYRCYPV---IPDLARYGCPAIGSVSVSDN---------LENPV-SCEARFTV 188

Query: 189 TVSKKSF-KEGEMNRTTLVETALREGFEVQY-ADDSGCYFCKENGGACGGNATQDYYCIC 248
            +       E  +N T+LV   +R GFEV+   D++ C  C  +   CG   T      C
Sbjct: 189 NIPTSFVPNEKRLNITSLVRD-VRNGFEVRLRIDENSCQECSSSHKYCGFTGTLPLETKC 248

Query: 249 ENGNINSFSCSSRIHTPASVGIAIGSINLIIFIVILSILLLVRWRKKKKRNSKKDEIEET 308
              N+      +R+ + A +   I  ++L+ F+V+  ++ ++R +K      ++D  E  
Sbjct: 249 RPLNL-----PTRLSSEAKIA-TIAGVSLLPFLVLTLVVHIIRKQKTSNDKGQQDLKEHI 308

Query: 309 IK-RYSTQIP-KRYTYSELKKITDSFKNKLGQGGFSIVYKGKLLDGRYVAVKLLNESR-E 368
            K R    I  K+Y+Y ++K+IT+SF   +G+GGF IVY+G L DGR VAVK+L + +  
Sbjct: 309 PKPRIKALIQLKQYSYEQVKRITNSFAEVVGRGGFGIVYRGTLSDGRMVAVKVLKDLKGN 368

Query: 369 DAQDFINEVVSITRTSHVNIVTFLGFCYEENKRALIYEYMPKGSLDKYIFQKGLQEKNKA 428
           + +DFINEV S+++TSHVNIVT LGFC E  KRA+IYE+M  GSLDK+I       K  +
Sbjct: 369 NGEDFINEVASMSQTSHVNIVTLLGFCSEGYKRAIIYEFMENGSLDKFI-----SSKKSS 428

Query: 429 KLNWNTLYSIIVGVARGLEYLHRGCNTRILHFDIKPHNILLDNEFCPKISDFGLAKQCKA 488
            ++W  LY I +GVARGLEYLH GC TRI+HFDIKP N+LLD+   PK+SDFGLAK C+ 
Sbjct: 429 TMDWRELYGIALGVARGLEYLHHGCRTRIVHFDIKPQNVLLDDNLSPKVSDFGLAKLCER 488

Query: 489 RESHVSMTCAKGTIGFMAPEVIIRSFGKVSHKSDVYSYGMLVLEMVGERKSPN----KGE 548
           +ES +S+   +GTIG++APEV  R +G VSHKSDVYSYGMLVL+++G R   +       
Sbjct: 489 KESILSLMDTRGTIGYIAPEVFSRVYGSVSHKSDVYSYGMLVLDIIGARNKTSTEDTTSS 548

Query: 549 SSEEYFPDWIYKDLAGSEIDRNCRQWGQTEEEEEMVRKMVVVSLYCIQTLPDDRPSMTEV 608
           +S  YFP+WIYKDL   E   N R      EE+E+ +KM +V L+CIQ  P DRP+M  V
Sbjct: 549 TSSMYFPEWIYKDL---EKGDNGRLIVNRSEEDEIAKKMTLVGLWCIQPWPLDRPAMNRV 605

Query: 609 VAMLEGSADGLQIPPKPVLFESPAASLSSS 629
           V M+EG+ D L++PP+PVL  S    L SS
Sbjct: 609 VEMMEGNLDALEVPPRPVLQCSVVPHLDSS 605

BLAST of Tan0008256 vs. TAIR 10
Match: AT1G66920.1 (Protein kinase superfamily protein )

HSP 1 Score: 410.6 bits (1054), Expect = 2.3e-114
Identity = 252/630 (40.00%), Postives = 358/630 (56.83%), Query Frame = 0

Query: 9   IFFFISFIAFQLSPCFSNDAGLDLFKACEMSYNCGNLVSISYPFWGNARPDFCGSSKFEL 68
           +  F+ F  F   PC S+   L     CE  + CGN ++  +PFWG  RP+ CG    EL
Sbjct: 9   LVLFLFFSLFYHLPCASSKQTLGW---CESQFQCGN-ITAGFPFWGGNRPEVCGHPLLEL 68

Query: 69  KCNNNKTTTIKINSLDYYVLNINQSDQTMRMARSDLFDDYC-PKSKIETAKLDPSQFSYT 128
            C +N  T++ I+   Y+VL+IN +  T+R+AR+D     C        A L P  F   
Sbjct: 69  HCLDN-ITSLTISDHLYHVLSINHTYNTLRVARTDFLQSICLSPFPFANATLPPEIFDIL 128

Query: 129 EKDLNISIWYDCPMQEGVPEEFRFRCSGEGEKGNTNNYALEPEAMYLSRSIRGCKVKMEA 188
               +++++   P+   +P+  R+ C   G    ++N         L   +  C+ +   
Sbjct: 129 PTYKSVTLYRCYPV---IPDLARYGCPAIGSVSVSDN---------LENPV-SCEARFTV 188

Query: 189 TVSKKSF-KEGEMNRTTLVETALREGFEVQY-ADDSGCYFCKENGGACGGNATQDYYCIC 248
            +       E  +N T+LV   +R GFEV+   D++ C  C  +   CG   T      C
Sbjct: 189 NIPTSFVPNEKRLNITSLVRD-VRNGFEVRLRIDENSCQECSSSHKYCGFTGTLPLETKC 248

Query: 249 ENGNINSFSCSSRIHTPASVGIAIGSINLIIFIVILSILLLVRWRKKKKRNSKKDEIEET 308
              N         + TP      I  ++L+ F+V+  ++ ++R +K      ++D  E  
Sbjct: 249 RPLN---------LPTP-----TIAGVSLLPFLVLTLVVHIIRKQKTSNDKGQQDLKEHI 308

Query: 309 IK-RYSTQIP-KRYTYSELKKITDSFKNKLGQGGFSIVYKGKLLDGRYVAVKLLNESR-E 368
            K R    I  K+Y+Y ++K+IT+SF   +G+GGF IVY+G L DGR VAVK+L + +  
Sbjct: 309 PKPRIKALIQLKQYSYEQVKRITNSFAEVVGRGGFGIVYRGTLSDGRMVAVKVLKDLKGN 368

Query: 369 DAQDFINEVVSITRTSHVNIVTFLGFCYEENKRALIYEYMPKGSLDKYIFQKGLQEKNKA 428
           + +DFINEV S+++TSHVNIVT LGFC E  KRA+IYE+M  GSLDK+I       K  +
Sbjct: 369 NGEDFINEVASMSQTSHVNIVTLLGFCSEGYKRAIIYEFMENGSLDKFI-----SSKKSS 428

Query: 429 KLNWNTLYSIIVGVARGLEYLHRGCNTRILHFDIKPHNILLDNEFCPKISDFGLAKQCKA 488
            ++W  LY I +GVARGLEYLH GC TRI+HFDIKP N+LLD+   PK+SDFGLAK C+ 
Sbjct: 429 TMDWRELYGIALGVARGLEYLHHGCRTRIVHFDIKPQNVLLDDNLSPKVSDFGLAKLCER 488

Query: 489 RESHVSMTCAKGTIGFMAPEVIIRSFGKVSHKSDVYSYGMLVLEMVGERKSPN----KGE 548
           +ES +S+   +GTIG++APEV  R +G VSHKSDVYSYGMLVL+++G R   +       
Sbjct: 489 KESILSLMDTRGTIGYIAPEVFSRVYGSVSHKSDVYSYGMLVLDIIGARNKTSTEDTTSS 548

Query: 549 SSEEYFPDWIYKDLAGSEIDRNCRQWGQTEEEEEMVRKMVVVSLYCIQTLPDDRPSMTEV 608
           +S  YFP+WIYKDL   E   N R      EE+E+ +KM +V L+CIQ  P DRP+M  V
Sbjct: 549 TSSMYFPEWIYKDL---EKGDNGRLIVNRSEEDEIAKKMTLVGLWCIQPWPLDRPAMNRV 597

Query: 609 VAMLEGSADGLQIPPKPVLFESPAASLSSS 629
           V M+EG+ D L++PP+PVL  S    L SS
Sbjct: 609 VEMMEGNLDALEVPPRPVLQCSVVPHLDSS 597

BLAST of Tan0008256 vs. TAIR 10
Match: AT1G67000.1 (Protein kinase superfamily protein )

HSP 1 Score: 407.1 bits (1045), Expect = 2.5e-113
Identity = 253/634 (39.91%), Postives = 371/634 (58.52%), Query Frame = 0

Query: 31  DLFKACEMSYNCGNLVSISYPFWGNARPDFCGSSKFELKCNNNKTTTIKINSLDYYVLNI 90
           ++++ C  S++CG+   ++YP W   R + CG   F+L C+      I I S+ + +L+ 
Sbjct: 249 EVYRRCSESFSCGSQRDLNYPLWKPGREE-CGHPNFKLNCSGG-FAEINIASVKFRILDS 308

Query: 91  NQSDQTMRMARSDLFDDYCPKSKIETAKLDP--SQFSYTEKDLNISIWYDCPMQEG-VPE 150
            +S   +R+ARSD   D CP + +    ++        T +   ++++Y C      +P 
Sbjct: 309 YRS--LIRLARSDYIGDLCPANPLTAPFIEKFLPVLELTGETELLTLYYGCRFNSSDIPA 368

Query: 151 EFRFRCSG--EGEK----GNTNNYALEPEAMYLSRSIRGCKVKMEATVSKKSF--KEGEM 210
                  G  EG       N ++  L+     L+     CK  +    S  +    +   
Sbjct: 369 NIYVGELGCDEGRSYYVTRNLSSPLLDSSRGVLNNLREMCKRNVSVPASGPALFDLQTRP 428

Query: 211 NRTTLVETALREGFEVQYADDSGCYFCKENGGACGGNATQD-YYCICENGNI-----NSF 270
           N+  L + AL +GF +    D  C  C+ +GGACG N T   + C C++G       + F
Sbjct: 429 NQDNL-KMALDQGFRMLITSD--CERCRGSGGACGYNQTSSGFGCYCKDGKCGYEYDDGF 488

Query: 271 SCSSRIHTPASVG---IAIGSIN--LIIFIVILSILLLVRWRKKKKRNSKKDEIEETIKR 330
               R      V    IA+G++   +I+F+V+L     V+  +K+K +   DE+   +++
Sbjct: 489 FRRHRRFIATLVRYTFIALGALTGVVIVFLVLLCPCFRVQIFRKRKTS---DEVR--LQK 548

Query: 331 YSTQIP-KRYTYSELKKITDSFKNKLGQGGFSIVYKGKLLDGRYVAVKLLNESR-EDAQD 390
               IP K YTY+E+KK+T SF   +G+GGF IVY G L D   VAVK+L +S+  D +D
Sbjct: 549 LKALIPLKHYTYAEVKKMTKSFTEVVGRGGFGIVYSGTLSDSSMVAVKVLKDSKGTDGED 608

Query: 391 FINEVVSITRTSHVNIVTFLGFCYEENKRALIYEYMPKGSLDKYIFQKGLQEKNKAKLNW 450
           FINEV S+++TSHVNIV+ LGFC E ++RA+IYE++  GSLDK+I      +K+   L+ 
Sbjct: 609 FINEVASMSQTSHVNIVSLLGFCCEGSRRAIIYEFLGNGSLDKFI-----SDKSSVNLDL 668

Query: 451 NTLYSIIVGVARGLEYLHRGCNTRILHFDIKPHNILLDNEFCPKISDFGLAKQCKARESH 510
            TLY I +GVARGLEYLH GC TRI+HFDIKP N+LLD+  CPK+SDFGLAK C+ +ES 
Sbjct: 669 KTLYGIALGVARGLEYLHYGCKTRIVHFDIKPQNVLLDDNLCPKVSDFGLAKLCEKKESI 728

Query: 511 VSMTCAKGTIGFMAPEVIIRSFGKVSHKSDVYSYGMLVLEMVGERKSP-----NKGESSE 570
           +S+   +GTIG++APE+I R +G VSHKSDVYSYGMLVLEM+G RK       ++ + S 
Sbjct: 729 LSLLDTRGTIGYIAPEMISRLYGSVSHKSDVYSYGMLVLEMIGARKKERFDQNSRSDGSS 788

Query: 571 EYFPDWIYKDLAGSEI-------DRNCRQWGQTEEEEEMVRKMVVVSLYCIQTLPDDRPS 629
            YFP+WIYKDL  + I       +    + G + EEEE+ RKM +V L+CIQ+ P DRP 
Sbjct: 789 IYFPEWIYKDLEKANIKDIEKTENGGLIENGISSEEEEIARKMTLVGLWCIQSSPSDRPP 848

BLAST of Tan0008256 vs. TAIR 10
Match: AT5G38260.1 (Protein kinase superfamily protein )

HSP 1 Score: 396.7 bits (1018), Expect = 3.4e-110
Identity = 232/610 (38.03%), Postives = 358/610 (58.69%), Query Frame = 0

Query: 34  KACEMSYNCGNLVSISYPFWGNARPDFCGSSKFELKCNNNKTTTIKINSLDYYVLNINQS 93
           K C  ++ CG    + YPFW   R + CG   F++ C+ +      I+++ ++VL +N  
Sbjct: 35  KHCSPTFRCGKQTDLYYPFWSPDREE-CGHPVFKVNCSGD-FAEFTISTVKFHVLEMNYE 94

Query: 94  DQTMRMARSDLFDDYCPKSKIETAKLDPSQFSYTEKDLNISIWYDC--PMQEGVPEEFRF 153
            + +R+ R++  ++ CP    E   ++     + +     + +Y+C  P  + +   +  
Sbjct: 95  SRIIRLVRTEYLNNLCPWHP-ENRSINQEVLPFLQDTELGTFYYNCSGPTVDELANGYIR 154

Query: 154 RCSGEGEKGNTNNYALEP----EAMYLSRSIRGCKVKMEATVSKKSFKEGEMNRT-TLVE 213
           +   + E G  + +   P        L      C+  ++  VS+ + +    N++   ++
Sbjct: 155 QLGCDEEVGGKSYFVSSPSHPGNRAILDGLSASCERNVDIPVSRSAMETTATNQSLEAIK 214

Query: 214 TALREGFEVQYADDSGCYFCKENGGACGGN-ATQDYYCICENGNINSFSCSSRIHTPASV 273
             L  GFE+ +  +S C  C  + GACG N +++ + C C++            HT   +
Sbjct: 215 KVLDVGFELGF--NSDCSLCVASKGACGFNQSSKAFVCYCKD--------EPHEHTCGKM 274

Query: 274 GIAIGSINLIIFIVILSILLLVRWRKKKKRNSKKDEIEETIKRYSTQIPKRYTYSELKKI 333
           GI IG     +   ++++ LL  + +K++ +      +  +K    Q+ K+Y+Y+E++KI
Sbjct: 275 GIGIGLGCGFLGATLITVCLLCFFFQKRRTSHHLRPRDNNLKGL-VQL-KQYSYAEVRKI 334

Query: 334 TDSFKNKLGQGGFSIVYKGKLLDGRYVAVKLLNESREDAQDFINEVVSITRTSHVNIVTF 393
           T  F + LG+GGF  VY G L DGR VAVK+L + + + +DFINEV S+++TSHVNIV+ 
Sbjct: 335 TKLFSHTLGKGGFGTVYGGNLCDGRKVAVKILKDFKSNGEDFINEVASMSQTSHVNIVSL 394

Query: 394 LGFCYEENKRALIYEYMPKGSLDKYIFQKGLQEKNKAKLNWNTLYSIIVGVARGLEYLHR 453
           LGFCYE +KRA++YE++  GSLD++     L EK    L+ +TLY I +GVARGL+YLH 
Sbjct: 395 LGFCYEGSKRAIVYEFLENGSLDQF-----LSEKKSLNLDVSTLYRIALGVARGLDYLHH 454

Query: 454 GCNTRILHFDIKPHNILLDNEFCPKISDFGLAKQCKARESHVSMTCAKGTIGFMAPEVII 513
           GC TRI+HFDIKP NILLD+ FCPK+SDFGLAK C+ RES +S+  A+GTIG++APEV  
Sbjct: 455 GCKTRIVHFDIKPQNILLDDTFCPKVSDFGLAKLCEKRESILSLLDARGTIGYIAPEVFS 514

Query: 514 RSFGKVSHKSDVYSYGMLVLEMVG----ERKSPNKGESSEEYFPDWIYKDLAGSEIDRNC 573
             +G+VSHKSDVYSYGMLVLEM+G    E +      SS  YFPDWIYK+L   E   + 
Sbjct: 515 GMYGRVSHKSDVYSYGMLVLEMIGAKNKEIEETAASNSSSAYFPDWIYKNLENGE---DT 574

Query: 574 RQWGQ--TEEEEEMVRKMVVVSLYCIQTLPDDRPSMTEVVAMLEGSADGLQIPPKPVLFE 628
            ++G   + E++E+ +KM +V L+CIQ  P +RP M  +V M+EGS D L++PPKP +  
Sbjct: 575 WKFGDEISREDKEVAKKMTLVGLWCIQPSPLNRPPMNRIVEMMEGSLDVLEVPPKPSIHY 621