Tan0008249 (gene) Snake gourd v1

Overview
NameTan0008249
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionYpt/Rab-GAP domain of gyp1p superfamily protein
LocationLG01: 718947 .. 723705 (+)
RNA-Seq ExpressionTan0008249
SyntenyTan0008249
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTAAACAGAAAATCTCGGGGGGTTAGGGGATGATGTGGCATCGTTTTAAGCATCAATCCGTGAAGATGAGTAGAAGAAAGGGTCTGAAGAATCTGGCAGGGATGAGACTTGACGGAGTAATCTACTTCTGAGTGTTTCTTCTTCTTCTTCCATTCATCATCGTCTTCTTCTCTCTCCACCTTTCTGCATCTAGAGAGAGACAAATAGGTACAAAGAAAAGGAACTTGCTCTCCTTCCCTTCTCGTAAGTGGCAAAAGCCAAAGCCAACCCCATACCCCTTGCCCCAACCATACTTTAAAGTTCTCTTAGATTTACTTTCACAAAGGATAAGGCATTGCGAACGAAGCTCCCGATCTGTATCTTCTTCCCCTCTATGATCGCCACCGGCACCGACATCGCCCCCTCCTTCGGCGTCGGCGGCGGCTTCGTCTCCCGGATGATGGCGGGGGCGCGGCCGAATGCAGCCGTCGCGTTCACGGCCTTGGCGGGCCTCGCTGTGGTCGTCGTTCTCTTCTACTCTGCCAGTAGGTAAAACCCATAACGATCTTTTTTTCCCACAATTTCAAATTCTTCTCTAAGGGGCCAAAAAAGAAAGGGTTCATTCGTTAGTAAAGGGGGAAATCTACCCAATTCCTTTCTCCCCACTAGTCAATTTTCCATAGATGAGAGCTCTTAAACGAACTCCAACTCCGTCGTCTCCGGATCCGAATTCGAATTCTCCTCCTTCTTCATCTTCCTCGTCGTCAGCCTCATCGTCATGGGTTCATTTGCGTTCGGTTCTATTCGTTGTCACTTCCTCTTCACCGGCTTCTTCTTCCTCCTCTGATCGGTGAGTCTCTCTTTCCTTCCTCTTTTGCTTCCCATTTCTCTTATTCAGCTCAAAATTAAGCACAAACCTAGTTTTCGACAGAGATCTTGGGCATTTTGGGTTTTAGTCGTGTTTTTAGGGTTGATCTTTTGCTTAATCTAGGTGTTTAGGTCGTCTGAGTTCCTGGAGTACGTAGTTTCCTTTACATTTAGGTTCCTTTCTCACATTGTGGAGCTACTATCTGAATCTCCAGTTTGCTTCGGTCTTTTAGAGTACAGTACTCGATCATTTTACTCATCTTTTATTTTTCAAGTATAACTGGTGAATTATTAGTGTCATTATCCTCGGTTCTTAGGGAGTTACATAGATAGGTAATTGTGTATCATGCTGAGGGGACGCCATTTCATTGGATAAGATTAAAATGAATTGACTTTGCTGTATTAACCATGTCTTAGGGGACGCCTTAAATCGCCTTGGTCACGCAAGAAAAGAAAACACGCACTTTCACTGCAACAATGGAGGAGTTTGTTTACGCCAGATGGGAAACTTCGAGATGGCGGCATTAAGTTTTTAAAAAAAGTTCGCAGTGGAGTGAGTATAAATCACTTTATCTCACGTATTTTCTTTCAAGAGTGATAAGTTTTTTTTCCTTAACTTTGATATATTTATCGATTAGATGTTGATCTGATGATTTCTACTAGGGTGTAGATCCAAGTATTAGAGCAGAGGTCTGGCCGTTCCTTCTTGGAGTGTAAGTGAATACCCACCCCCTCTTCATTTTGTAATCTTGCATATGCTTCTTCTCTTGTATTATGTACAAAAATATCTTAGTTCTTTAGTCCATGGGGTATCTGGTTAGAATGCTGATTTTTCCGGCTCCTTACAAAGTGCATAATCATAACACGAATATATGAGCTATTTTAATCAGAACCTCAGATTGTGTGGTCAATGTAATCTTAATGAGCTAGAAATATCTAACATGGTTTGTTGATCTTTTCCAAAGTTTCCCTTCTCAAACATTGTTCGAGTAGAAAACATGAATTCTTGACTCTTGGGTCTTGTGTATAAATTTGTCTGGTCTTCTCTACTTTGCTCTTTTATCCTTGCTATCATTGTGTGTGGGCAAGAAAGGAGTGGCTTGATGCAGGTTTCTCTTTCTTTCTATCTAGTTCATTATTATTGCTTTTATATCCTCCTCCTCCTCCTCCTCATGAATTTTTCCATCTCATCCTTTTCTTATTGCATATCCGTGCCAGCGAGATGTCTCTTTGAGATTCACCCCTATTTTGCTTCCCTCTCTCTTACCAAACACTTCACATCCCATCTGTATTATATAATAGCAAGGGTATGTTTTTGCTCTAACCGCGGTAAATGTATGGGTCTCAATATGTCCCCGATGAACTTAAAAGGCTGTCCTAGTTTGTCTGCAATTCTAAAGTATAGTGAAAGGTTGGAAGTTCTGCTTGTTGGTCCCTTGATCACAGTGTTTTGGAATCAATGATCGGTCAAAATTGGAGAGACTGTGTTACACGGGTTTGTATCGTACAAACATTGAAGGGTATAGAGCCAGTCCTACATAAAAAAAAAAGGATGCTGCATTCTCATGCTTCTTCCCAGCATAACCTTTTCTATTTTACATGCAGCTGTCTTATATTTTCTTCACTTTTTATTAATGATTGGATGATATTTCTTTTATAGTCTTTTGGGCGAGGTTCTCTCTACATGTGGCCCCTAGGTTGTTGCCCTTTCGTCGTAATACATTCACCATTTCTTATTTTAAAAAAGTGTGTATATATTTTGTTCACTTTTCTATAGGACTAGTCCTCGACACCCTCATTTTGAAGATTCATGTCATGCTATATATACATGCTGTGCTGCTATGCTATGTGTATGCGATGCTTGGTATTGTTCAAGATCATGTGGTGTGCAAAATGTGTATTGACATGACTACTTATGTTTTCAACAGTTACAACTTGAGCAGTTGTGAAGAAGAAAGAGATGCCGTAAGAGTACAGAAGAGGTATATGTTTTCAAGTGCTAATATTATTTCATTCTATTTTTCTTGGATAAGTTTGTGGGGGTATTTTACCCACCTGCAGCTGTGTTCTGAGTTGGCATTTTAAACTTTAAGGTGGTCAACATATATAATGCAGGAAAGAATATGAAAGACTTCGAAAACAATGCCGATCATTACTGAAGTCTGGGGATGAGAGTATGAAGTTGAATGATGATGATATGAACTGCAACAAGGAGAGGGACAATCAGCATGCTGCACATGGGGATGACTCTCCAATGAATTGCAACAAGGAGGGGGACAATCAGCATGTTGCTCATGGTGATGATGACTCTCCTAGTTTGGAAGATGTGGTTAGTGCTAGAGAATCTATTTCTAGTGATGAAAGGGGTTCGAACTTTAGGTACTTGGATGGAACCTCGGGGGTTTTGTTGGAAGAGGATGACAGTTCTAGAGGGTTGACAAATGCTGATGCTTCTGTTCTAAATACTGAATCATCTGACTCAGATTCGTCTGAGGATCCTGAAGTTAGTCAAACATTCCCTTCCTCTGATGGTAGAGAAGATAATGGTCCTGATTTCACTTCCAAAAACTCGTCTCCCTTGGTAACAGAGGTTACATCCAAATTTCGCAGTAATGAAGACTTTACAACATGGCAGCGGATCATCCGTCTTGATGCAGTACGTGCAAATGCAGAATGGATAGTGTACGCACCAACCCAAGCAGCGGTATCAGATGATAAGGCTAGACATTGTGCAGAGGTTGTTGGCTTGAAAGATTACGATCACCTAGAGTCCTGCAGGATCTTTCATGCTGCTAGGTTGGTCACCATTCTTGAAGCATACGCTCTTTATGACCCTGAAATTGGGTATTGCCAAGGAATGAGTGATCTGCTTTCTCCTATAATTACTGTGATAACTGAAGATCATGAGGCATTCTGGTGCTTTGTAGGCTTCATGCGGAAAGCTCGGCATAACTTTAGGCTTGATGAGGTTGGGATTCGAAAGCAGCTGAACATTGTATCTAGGATCATCAAATTCAAGGACTCCCACCTTTACAGACACCTGCAAGACCTCCAAGCGGAGGATTGCTTTTTCGTTTATAGGATGGTTGTGGTACTTTTTAGAAGGGAATTAACATTTGAACAGACGTTGTGCCTTTGGGAAGTCATGTGGGCCGATCAGGCAGCTATTAGAGCTGGTGTAGGTAAATCAGCTTGGAGCAGGATTAGGCAACGAGCCCCACCCACGGAGGATCTGCTGCTCTATGCAATTGCTGCCTCGGTATTACAGAAGAGGAAGTTGATTATAGAGAAATACAACAGTATGGACGAAATAATAAGGGAGTGTAACAGCATGGCCGGGCAACTTGATGTATGGAAACTATTGGACGATGCTCATGATTTGGTGGTGACCCTCCATGAGAAGATAGAAACCTCGTTTAATGAGTAAATGCCCAATTGCTGGATTGTTAATTTACGTGCCATTCTTCCTGGATCCTTATCTAGGCTTCTGCTCGGAGAAAGTGCATCTCTCTTTGCTTATTCAATTCAAGGGGGCTAGCAGGATGTTTCTTTTGCTCACTTTCTTGTAGCTCACTCAAGCTCAATCAAGTTGTAGTGACAGATTTTAATGACGAATCACTATAATTTGCATAATATTGCATCCTCACCTTCATCTATTTCAGACTGGCTGCCTGCCATAATAGCATAGCACTAAACGGGAGTAGTGTATGGCGATGCACAAGTCAACGAATTTTCAAAAGTAGAAAATATCTTGTTCTTGGTGTACGTTTAGTTGGAGTAATAACTACTGCCCTCCATTGATGGGTTACTTTTTTTTTTTTTTTTTGGTTAACCTGCTTCTTGTACCTCTAATCTGTTTCCAGTTGACCAAAATCTATACTCAAAAGTCATTAAAGCAGAATAGGCATTGGA

mRNA sequence

TTTAAACAGAAAATCTCGGGGGGTTAGGGGATGATGTGGCATCGTTTTAAGCATCAATCCGTGAAGATGAGTAGAAGAAAGGGTCTGAAGAATCTGGCAGGGATGAGACTTGACGGAGTAATCTACTTCTGAGTGTTTCTTCTTCTTCTTCCATTCATCATCGTCTTCTTCTCTCTCCACCTTTCTGCATCTAGAGAGAGACAAATAGGTACAAAGAAAAGGAACTTGCTCTCCTTCCCTTCTCGTAAGTGGCAAAAGCCAAAGCCAACCCCATACCCCTTGCCCCAACCATACTTTAAAGTTCTCTTAGATTTACTTTCACAAAGGATAAGGCATTGCGAACGAAGCTCCCGATCTGTATCTTCTTCCCCTCTATGATCGCCACCGGCACCGACATCGCCCCCTCCTTCGGCGTCGGCGGCGGCTTCGTCTCCCGGATGATGGCGGGGGCGCGGCCGAATGCAGCCGTCGCGTTCACGGCCTTGGCGGGCCTCGCTGTGGTCGTCGTTCTCTTCTACTCTGCCAGTAGGTAAAACCCATAACGATCTTTTTTTCCCACAATTTCAAATTCTTCTCTAAGGGGCCAAAAAAGAAAGGGTTCATTCGTTAGTAAAGGGGGAAATCTACCCAATTCCTTTCTCCCCACTAGTCAATTTTCCATAGATGAGAGCTCTTAAACGAACTCCAACTCCGTCGTCTCCGGATCCGAATTCGAATTCTCCTCCTTCTTCATCTTCCTCGTCGTCAGCCTCATCGTCATGGGTTCATTTGCGTTCGGTTCTATTCGTTGTCACTTCCTCTTCACCGGCTTCTTCTTCCTCCTCTGATCGGGGACGCCTTAAATCGCCTTGGTCACGCAAGAAAAGAAAACACGCACTTTCACTGCAACAATGGAGGAGTTTGTTTACGCCAGATGGGAAACTTCGAGATGGCGGCATTAAGTTTTTAAAAAAAGTTCGCAGTGGAGGTGTAGATCCAAGTATTAGAGCAGAGGTCTGGCCGTTCCTTCTTGGAGTTTACAACTTGAGCAGTTGTGAAGAAGAAAGAGATGCCGTAAGAGTACAGAAGAGGAAAGAATATGAAAGACTTCGAAAACAATGCCGATCATTACTGAAGTCTGGGGATGAGAGTATGAAGTTGAATGATGATGATATGAACTGCAACAAGGAGAGGGACAATCAGCATGCTGCACATGGGGATGACTCTCCAATGAATTGCAACAAGGAGGGGGACAATCAGCATGTTGCTCATGGTGATGATGACTCTCCTAGTTTGGAAGATGTGGTTAGTGCTAGAGAATCTATTTCTAGTGATGAAAGGGGTTCGAACTTTAGGTACTTGGATGGAACCTCGGGGGTTTTGTTGGAAGAGGATGACAGTTCTAGAGGGTTGACAAATGCTGATGCTTCTGTTCTAAATACTGAATCATCTGACTCAGATTCGTCTGAGGATCCTGAAGTTAGTCAAACATTCCCTTCCTCTGATGGTAGAGAAGATAATGGTCCTGATTTCACTTCCAAAAACTCGTCTCCCTTGGTAACAGAGGTTACATCCAAATTTCGCAGTAATGAAGACTTTACAACATGGCAGCGGATCATCCGTCTTGATGCAGTACGTGCAAATGCAGAATGGATAGTGTACGCACCAACCCAAGCAGCGGTATCAGATGATAAGGCTAGACATTGTGCAGAGGTTGTTGGCTTGAAAGATTACGATCACCTAGAGTCCTGCAGGATCTTTCATGCTGCTAGGTTGGTCACCATTCTTGAAGCATACGCTCTTTATGACCCTGAAATTGGGTATTGCCAAGGAATGAGTGATCTGCTTTCTCCTATAATTACTGTGATAACTGAAGATCATGAGGCATTCTGGTGCTTTGTAGGCTTCATGCGGAAAGCTCGGCATAACTTTAGGCTTGATGAGGTTGGGATTCGAAAGCAGCTGAACATTGTATCTAGGATCATCAAATTCAAGGACTCCCACCTTTACAGACACCTGCAAGACCTCCAAGCGGAGGATTGCTTTTTCGTTTATAGGATGGTTGTGGTACTTTTTAGAAGGGAATTAACATTTGAACAGACGTTGTGCCTTTGGGAAGTCATGTGGGCCGATCAGGCAGCTATTAGAGCTGGTGTAGGTAAATCAGCTTGGAGCAGGATTAGGCAACGAGCCCCACCCACGGAGGATCTGCTGCTCTATGCAATTGCTGCCTCGGTATTACAGAAGAGGAAGTTGATTATAGAGAAATACAACAGTATGGACGAAATAATAAGGGAGTGTAACAGCATGGCCGGGCAACTTGATGTATGGAAACTATTGGACGATGCTCATGATTTGGTGGTGACCCTCCATGAGAAGATAGAAACCTCGTTTAATGAGTAAATGCCCAATTGCTGGATTGTTAATTTACGTGCCATTCTTCCTGGATCCTTATCTAGGCTTCTGCTCGGAGAAAGTGCATCTCTCTTTGCTTATTCAATTCAAGGGGGCTAGCAGGATGTTTCTTTTGCTCACTTTCTTGTAGCTCACTCAAGCTCAATCAAGTTGTAGTGACAGATTTTAATGACGAATCACTATAATTTGCATAATATTGCATCCTCACCTTCATCTATTTCAGACTGGCTGCCTGCCATAATAGCATAGCACTAAACGGGAGTAGTGTATGGCGATGCACAAGTCAACGAATTTTCAAAAGTAGAAAATATCTTGTTCTTGGTGTACGTTTAGTTGGAGTAATAACTACTGCCCTCCATTGATGGGTTACTTTTTTTTTTTTTTTTTGGTTAACCTGCTTCTTGTACCTCTAATCTGTTTCCAGTTGACCAAAATCTATACTCAAAAGTCATTAAAGCAGAATAGGCATTGGA

Coding sequence (CDS)

ATGAGAGCTCTTAAACGAACTCCAACTCCGTCGTCTCCGGATCCGAATTCGAATTCTCCTCCTTCTTCATCTTCCTCGTCGTCAGCCTCATCGTCATGGGTTCATTTGCGTTCGGTTCTATTCGTTGTCACTTCCTCTTCACCGGCTTCTTCTTCCTCCTCTGATCGGGGACGCCTTAAATCGCCTTGGTCACGCAAGAAAAGAAAACACGCACTTTCACTGCAACAATGGAGGAGTTTGTTTACGCCAGATGGGAAACTTCGAGATGGCGGCATTAAGTTTTTAAAAAAAGTTCGCAGTGGAGGTGTAGATCCAAGTATTAGAGCAGAGGTCTGGCCGTTCCTTCTTGGAGTTTACAACTTGAGCAGTTGTGAAGAAGAAAGAGATGCCGTAAGAGTACAGAAGAGGAAAGAATATGAAAGACTTCGAAAACAATGCCGATCATTACTGAAGTCTGGGGATGAGAGTATGAAGTTGAATGATGATGATATGAACTGCAACAAGGAGAGGGACAATCAGCATGCTGCACATGGGGATGACTCTCCAATGAATTGCAACAAGGAGGGGGACAATCAGCATGTTGCTCATGGTGATGATGACTCTCCTAGTTTGGAAGATGTGGTTAGTGCTAGAGAATCTATTTCTAGTGATGAAAGGGGTTCGAACTTTAGGTACTTGGATGGAACCTCGGGGGTTTTGTTGGAAGAGGATGACAGTTCTAGAGGGTTGACAAATGCTGATGCTTCTGTTCTAAATACTGAATCATCTGACTCAGATTCGTCTGAGGATCCTGAAGTTAGTCAAACATTCCCTTCCTCTGATGGTAGAGAAGATAATGGTCCTGATTTCACTTCCAAAAACTCGTCTCCCTTGGTAACAGAGGTTACATCCAAATTTCGCAGTAATGAAGACTTTACAACATGGCAGCGGATCATCCGTCTTGATGCAGTACGTGCAAATGCAGAATGGATAGTGTACGCACCAACCCAAGCAGCGGTATCAGATGATAAGGCTAGACATTGTGCAGAGGTTGTTGGCTTGAAAGATTACGATCACCTAGAGTCCTGCAGGATCTTTCATGCTGCTAGGTTGGTCACCATTCTTGAAGCATACGCTCTTTATGACCCTGAAATTGGGTATTGCCAAGGAATGAGTGATCTGCTTTCTCCTATAATTACTGTGATAACTGAAGATCATGAGGCATTCTGGTGCTTTGTAGGCTTCATGCGGAAAGCTCGGCATAACTTTAGGCTTGATGAGGTTGGGATTCGAAAGCAGCTGAACATTGTATCTAGGATCATCAAATTCAAGGACTCCCACCTTTACAGACACCTGCAAGACCTCCAAGCGGAGGATTGCTTTTTCGTTTATAGGATGGTTGTGGTACTTTTTAGAAGGGAATTAACATTTGAACAGACGTTGTGCCTTTGGGAAGTCATGTGGGCCGATCAGGCAGCTATTAGAGCTGGTGTAGGTAAATCAGCTTGGAGCAGGATTAGGCAACGAGCCCCACCCACGGAGGATCTGCTGCTCTATGCAATTGCTGCCTCGGTATTACAGAAGAGGAAGTTGATTATAGAGAAATACAACAGTATGGACGAAATAATAAGGGAGTGTAACAGCATGGCCGGGCAACTTGATGTATGGAAACTATTGGACGATGCTCATGATTTGGTGGTGACCCTCCATGAGAAGATAGAAACCTCGTTTAATGAGTAA

Protein sequence

MRALKRTPTPSSPDPNSNSPPSSSSSSSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLKSPWSRKKRKHALSLQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLGVYNLSSCEEERDAVRVQKRKEYERLRKQCRSLLKSGDESMKLNDDDMNCNKERDNQHAAHGDDSPMNCNKEGDNQHVAHGDDDSPSLEDVVSARESISSDERGSNFRYLDGTSGVLLEEDDSSRGLTNADASVLNTESSDSDSSEDPEVSQTFPSSDGREDNGPDFTSKNSSPLVTEVTSKFRSNEDFTTWQRIIRLDAVRANAEWIVYAPTQAAVSDDKARHCAEVVGLKDYDHLESCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSMDEIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKIETSFNE
Homology
BLAST of Tan0008249 vs. ExPASy Swiss-Prot
Match: Q94BY9 (Rab GTPase-activating protein 22 OS=Arabidopsis thaliana OX=3702 GN=RABGAP22 PE=1 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 2.8e-177
Identity = 353/582 (60.65%), Postives = 426/582 (73.20%), Query Frame = 0

Query: 3   ALKRTPTPSSPDPNSNSPPSS-------SSSSSASSSWVHLRSVLFVVTSSSPASSSSSD 62
           +L  + + S P  +S+SPPSS       S+SSS+SSSW+HLRSVLFV   SSP+S +SSD
Sbjct: 21  SLPSSSSSSLPSSSSSSPPSSNSNSYSNSNSSSSSSSWIHLRSVLFVANLSSPSSVTSSD 80

Query: 63  RGRLKSPWSRKKRKHALSLQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFL 122
           R R KSPWSR+KRK AL+  QWRSLFTP+GKLRDGG+ FLKKVRS GVDPSIRAEVW FL
Sbjct: 81  R-RRKSPWSRRKRKWALTPHQWRSLFTPEGKLRDGGVGFLKKVRSRGVDPSIRAEVWLFL 140

Query: 123 LGVYNLSSCEEERDAVRVQKRKEYERLRKQCRSLLKSGDESMKLNDDDMNCNKERDNQHA 182
           LGVY+L+S  EER+AV+ QKRKEYE+L+++C+ LLK G             N   DN   
Sbjct: 141 LGVYDLNSTSEEREAVKTQKRKEYEKLQRRCQMLLKCG-------------NGSTDNLEE 200

Query: 183 AHGDDSPMNCNKEGDNQHVAHGDDDSPSLEDVVSARESISSDERGSNFRYLDGTSGVLLE 242
              D++   C +  D+  +        + +DVVSA                         
Sbjct: 201 LPSDEANSQCVRFVDDYKIT----GPMTSQDVVSA------------------------- 260

Query: 243 EDDSSRGLTNADASVLNTESSDSDSSEDPE---VSQTFPSSDGR---EDNGPDFTSKNSS 302
                          LNT+SSD+DS ED E   +  +F  SD +   EDN  + + +NSS
Sbjct: 261 ---------------LNTDSSDTDSCEDNEDVLLLSSFAHSDEKKPEEDNSNNNSEENSS 320

Query: 303 PLV---TEVTSKFRSNEDFTTWQRIIRLDAVRANAEWIVYAPTQAAVSDDKARHCAEVVG 362
            LV   +EV  +   +EDF+TWQRIIRLDA+RA++EW  Y+P   A+++ KAR  AE VG
Sbjct: 321 LLVAAASEVQVEVAVHEDFSTWQRIIRLDALRADSEWANYSPYSTAITESKARRLAESVG 380

Query: 363 LKDYDHLESCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFV 422
           LKDYDHLESCR++HAARLV ILEAYA+YDPEIGYCQGMSDLLSPI+ VI+EDHEAFWCFV
Sbjct: 381 LKDYDHLESCRLYHAARLVAILEAYAMYDPEIGYCQGMSDLLSPILAVISEDHEAFWCFV 440

Query: 423 GFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVVLFRR 482
           GFM+KARHNFRLDE GI++QL+IVS+IIK KDS LY+HL++LQAEDC FVYRMV+V+FRR
Sbjct: 441 GFMKKARHNFRLDEAGIQRQLSIVSKIIKNKDSQLYKHLENLQAEDCSFVYRMVLVMFRR 500

Query: 483 ELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLII 542
           EL+FEQTLCLWEVMWADQAAIRAGVGKS WSRIRQ+APPT+DLLLYAIAA VL +RKLII
Sbjct: 501 ELSFEQTLCLWEVMWADQAAIRAGVGKSPWSRIRQQAPPTDDLLLYAIAALVL-RRKLII 543

Query: 543 EKYNSMDEIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKIET 569
           +KY+SMDEI+ ECNSMAGQL+VWKLLDDAH LVVTLH+KIET
Sbjct: 561 QKYSSMDEIVEECNSMAGQLNVWKLLDDAHHLVVTLHDKIET 543

BLAST of Tan0008249 vs. ExPASy Swiss-Prot
Match: P09379 (GTPase-activating protein GYP7 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) OX=284591 GN=GYP7 PE=3 SV=2)

HSP 1 Score: 119.0 bits (297), Expect = 1.7e-25
Identity = 63/208 (30.29%), Postives = 108/208 (51.92%), Query Frame = 0

Query: 360 HAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARHNFRLD 419
           H  +L  +L  Y  ++  +GY QGMSDLLSP+  V+ +D  AFW F  FM +   N+  D
Sbjct: 495 HLIQLRDMLITYNQHNKNLGYVQGMSDLLSPLYVVLQDDTLAFWAFSAFMERMERNYLRD 554

Query: 420 EVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEV 479
           + G+R QL  +  +++F    LY+HL+  ++ + FF +RM++V F+REL ++  L LWEV
Sbjct: 555 QSGMRNQLLCLDHLVQFMLPSLYKHLEKTESTNLFFFFRMLLVWFKRELLWDDVLRLWEV 614

Query: 480 MWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSMDEIIREC 539
           +W D                      +   +L+   A + + + ++I+     DEI++  
Sbjct: 615 LWTDYL--------------------SSQFVLFVCLAILDKHKDVMIDHLAGFDEILKYM 674

Query: 540 NSMAGQLDVWKLLDDAHDLVVTLHEKIE 568
           N ++  +D+ +LL  A  L       +E
Sbjct: 675 NELSMTIDLDELLVRAELLFYRFRRTVE 682


HSP 2 Score: 52.4 bits (124), Expect = 2.0e-05
Identity = 31/93 (33.33%), Postives = 52/93 (55.91%), Query Frame = 0

Query: 54  SDRG-RLKSPWSRKKRKHALSLQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVW 113
           SD G  L    +  +R++ +SL +W + F  +G+L     +  +++  GG+ P++R E W
Sbjct: 339 SDEGFELIDAENNPQRRNEVSLAEWNAFFDYNGRLIVTVNEVKERIFHGGLAPAVRPEGW 398

Query: 114 PFLLGVYNLSSCEEERDAVRVQKRKEYERLRKQ 146
            FLLGVY   S   ER  +  + R +Y RL+K+
Sbjct: 399 LFLLGVYPWDSTAAERKELVSKLRVDYNRLKKE 431

BLAST of Tan0008249 vs. ExPASy Swiss-Prot
Match: Q8TC07 (TBC1 domain family member 15 OS=Homo sapiens OX=9606 GN=TBC1D15 PE=1 SV=2)

HSP 1 Score: 109.8 bits (273), Expect = 1.1e-22
Identity = 57/186 (30.65%), Postives = 99/186 (53.23%), Query Frame = 0

Query: 367 ILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARHNFRLDEVGIRKQ 426
           IL  Y +YD ++GY QGMSDLLSP++ V+  + +AFWCF  +M +   NF     G++ Q
Sbjct: 439 ILMTYCMYDFDLGYVQGMSDLLSPLLYVMENEVDAFWCFASYMDQMHQNFEEQMQGMKTQ 498

Query: 427 LNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVMWADQAA 486
           L  +S +++  DS    +L+   +   +F +R +++ F+RE +F   L LWEVMW +   
Sbjct: 499 LIQLSTLLRLLDSGFCSYLESQDSGYLYFCFRWLLIRFKREFSFLDILRLWEVMWTEL-- 558

Query: 487 IRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSMDEIIRECNSMAGQL 546
                             P  +  L    A +  +++ I+EK+   +EI++  N ++ ++
Sbjct: 559 ------------------PCTNFHLLLCCAILESEKQQIMEKHYGFNEILKHINELSMKI 604

Query: 547 DVWKLL 553
           DV  +L
Sbjct: 619 DVEDIL 604

BLAST of Tan0008249 vs. ExPASy Swiss-Prot
Match: Q6FWI1 (GTPase-activating protein GYP7 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=GYP7 PE=3 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 4.0e-22
Identity = 58/203 (28.57%), Postives = 103/203 (50.74%), Query Frame = 0

Query: 363 RLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARHNFRLDEVG 422
           +L  IL  Y +++P +GY QGM+DLLSP+  +I ++   FWCF  FM +   NF  D+ G
Sbjct: 502 KLADILTTYNIFNPNLGYVQGMTDLLSPLYYIIRDEETTFWCFTNFMERMERNFLRDQSG 561

Query: 423 IRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVMWA 482
           IR Q+  ++ + +     L  HLQ   + D FF +RM++V F+RE  ++    +WEV + 
Sbjct: 562 IRDQMLALTDLCQLMLPRLSAHLQKCDSSDLFFCFRMLLVWFKREFNYDDIFNIWEVFFT 621

Query: 483 DQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKL-IIEKYNSMDEIIRECNS 542
           D  +                   ++  L + +A  +LQK    I+    + D++I+  N 
Sbjct: 622 DFYS-------------------SQYQLFFMLA--ILQKNSSPIVNNLQTFDQVIKYFND 681

Query: 543 MAGQLDVWKLLDDAHDLVVTLHE 565
           +  +++   L+  +  L +  H+
Sbjct: 682 LNSKMNWRDLMVRSELLFIQFHK 683


HSP 2 Score: 49.3 bits (116), Expect = 1.7e-04
Identity = 41/143 (28.67%), Postives = 72/143 (50.35%), Query Frame = 0

Query: 67  KRKHALSLQQWRSLFTPDGKLRDGGIKFLKKVRSGGV-DPSIRAEVWPFLLGVYNLSSCE 126
           +R H ++ Q+W SLF  +G+L     +    +  GG+ D + R EVWPFLLGVY   S E
Sbjct: 355 ERNHPMTKQKWNSLFDSEGRLTVTVNEVKDYIFHGGLADDATRKEVWPFLLGVYPWDSSE 414

Query: 127 EERDAVRVQKRKEYERLRKQCRSLLKSGDESMKLNDDDMNCNKER--DNQHAAHGDDSPM 186
           +ER  +R     EY  L++      K  D  + L++D+    K++    +     +D  +
Sbjct: 415 DERKQLRKALHDEYMELKQ------KWVDREVNLDNDEEEYWKDQLFRIEKDVKRNDRNI 474

Query: 187 NCNKEGDNQHVAHGDDDSPSLED 207
           +  K   + ++   +D +P+ +D
Sbjct: 475 DIYKYNTSDNLPFPEDTAPTTDD 491

BLAST of Tan0008249 vs. ExPASy Swiss-Prot
Match: Q9UUH7 (GTPase-activating protein gyp7 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=gyp7 PE=3 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 8.9e-22
Identity = 55/180 (30.56%), Postives = 99/180 (55.00%), Query Frame = 0

Query: 367 ILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARHNFRLDEVGIRKQ 426
           IL  Y  YD E+GY QGMSDLL+PI     ++   FW  VG M++   NF  D+ G+ +Q
Sbjct: 516 ILLTYNEYDTELGYVQGMSDLLAPIYVTFNDNALTFWGMVGLMKRLHFNFLRDQSGMHRQ 575

Query: 427 LNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVMWADQAA 486
           L+ +  +I+F D  L+ HL+   + + F  +RM+++ F+RE  +E  L LW+V++ +  +
Sbjct: 576 LDTLRLLIEFMDPELFAHLEKTDSSNLFCFFRMLLIYFKREFDWEVLLKLWDVLFTNYLS 635

Query: 487 IRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSMDEIIRECNSMAGQL 546
               +                  + YAIA    + R++++ + ++ DE+++  N ++G+L
Sbjct: 636 YDYHI-----------------FVAYAIAE---RHREVLLNQTSAFDEVLKYFNELSGKL 675


HSP 2 Score: 67.8 bits (164), Expect = 4.6e-10
Identity = 34/81 (41.98%), Postives = 49/81 (60.49%), Query Frame = 0

Query: 65  RKKRKHALSLQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLGVYNLSSC 124
           R KR   LS++QW S+F   GKL+    + L  +  GG+ PS+R EVWPFLL VY   S 
Sbjct: 374 RVKRDDPLSVEQWNSMFNAHGKLQVDVHRVLGIIFHGGIQPSLRKEVWPFLLSVYPWDST 433

Query: 125 EEERDAVRVQKRKEYERLRKQ 146
            EER  + +  ++EY  L+++
Sbjct: 434 SEERRVIYLSLQEEYCTLKRK 454

BLAST of Tan0008249 vs. NCBI nr
Match: XP_022968472.1 (small G protein signaling modulator 2-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 1001.9 bits (2589), Expect = 2.2e-288
Identity = 530/588 (90.14%), Postives = 543/588 (92.35%), Query Frame = 0

Query: 1   MRALKRTPTPSSPDPNSNSPPSSSSSSSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLK 60
           MRALK+TPTPSSPDPNSNSPPSSSS SSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLK
Sbjct: 1   MRALKQTPTPSSPDPNSNSPPSSSSLSSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLK 60

Query: 61  SPWSRKKRKHALSLQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLGVYN 120
           SPWSRKKRKHALS QQWRSL+TPDGKLRDGG+KFLKKVRSGGVDPSIRAEVWPFLLGVY+
Sbjct: 61  SPWSRKKRKHALSPQQWRSLYTPDGKLRDGGLKFLKKVRSGGVDPSIRAEVWPFLLGVYD 120

Query: 121 LSSCEEERDAVRVQKRKEYERLRKQCRSLLKSGDESMKLNDDD----------------- 180
           L+S EEERDAVRVQKRKEYERLRKQCRSLLK GDESMKLNDDD                 
Sbjct: 121 LTSSEEERDAVRVQKRKEYERLRKQCRSLLKFGDESMKLNDDDMNCHKEGGIQHAAHGDG 180

Query: 181 --MNCNKERDNQHAAHGDDSPMNCNKEGDNQHVAHGDDDSPSLEDVVSARESISSDERGS 240
             MNCNKE DNQH AHGDDS MNCNKEGDNQHVAHG DDSPSL DV+SARESISSDERG 
Sbjct: 181 SSMNCNKEGDNQHVAHGDDSSMNCNKEGDNQHVAHG-DDSPSLGDVISARESISSDERGL 240

Query: 241 NFRYLDGTSGVLLEEDDSSRGLTNADASVLNTESSDSDSSEDPEVSQTFPSSDGREDNGP 300
           N       SGVLLEEDDSSRG+TNAD+SVLNTESSDSDSS DPEVS TFPSSDGREDN P
Sbjct: 241 N-------SGVLLEEDDSSRGMTNADSSVLNTESSDSDSSGDPEVSHTFPSSDGREDNDP 300

Query: 301 DFTSKNSSPLVTEVTSKFRSNEDFTTWQRIIRLDAVRANAEWIVYAPTQAAVSDDKARHC 360
           DFTSKN SPLV EVTSKFRSNEDFTTWQRIIRLDAVRANAEWI YAP+QAAVSDDKARH 
Sbjct: 301 DFTSKNLSPLV-EVTSKFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKARHL 360

Query: 361 AEVVGLKDYDHLESCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEA 420
           AEVVGLKDYDHLES RIFHA+RLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEA
Sbjct: 361 AEVVGLKDYDHLESSRIFHASRLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEA 420

Query: 421 FWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVV 480
           FWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQ+L+AEDCFFVYRMVV
Sbjct: 421 FWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQNLEAEDCFFVYRMVV 480

Query: 481 VLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQK 540
           VLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQK
Sbjct: 481 VLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQK 540

Query: 541 RKLIIEKYNSMDEIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKIETS 570
           RKLIIE+YNSMDEIIRECNSMAGQLDVWKLLD AHDLVVTLHEKIETS
Sbjct: 541 RKLIIERYNSMDEIIRECNSMAGQLDVWKLLDGAHDLVVTLHEKIETS 579

BLAST of Tan0008249 vs. NCBI nr
Match: KAG7013330.1 (GYP7 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1001.5 bits (2588), Expect = 2.9e-288
Identity = 529/587 (90.12%), Postives = 543/587 (92.50%), Query Frame = 0

Query: 1   MRALKRTPTPSSPDPNSNSPPSSSSSSSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLK 60
           MRALK+TPTPSSPDPNSNSPPSSSS SSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLK
Sbjct: 1   MRALKQTPTPSSPDPNSNSPPSSSSLSSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLK 60

Query: 61  SPWSRKKRKHALSLQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLGVYN 120
           SPWSRKKRKHALS QQWRSL+TPDGKLRDGG+KFLKKVRSGGVDPSIRAEVWPFLLGVY+
Sbjct: 61  SPWSRKKRKHALSPQQWRSLYTPDGKLRDGGLKFLKKVRSGGVDPSIRAEVWPFLLGVYD 120

Query: 121 LSSCEEERDAVRVQKRKEYERLRKQCRSLLKSGDESMKLNDDDMNCNKERDNQHAAHGDD 180
           L+S EEERDAVRVQKRKEYERLRKQCRSLLK GDESMKLNDDDMNC+KE   QHAAHGDD
Sbjct: 121 LTSSEEERDAVRVQKRKEYERLRKQCRSLLKFGDESMKLNDDDMNCHKEGGIQHAAHGDD 180

Query: 181 SPMNCNKEGDNQHVAHGD------------------DDSPSLEDVVSARESISSDERGSN 240
           S MNCNKEGDNQHVAHGD                  DDSPSL DV+SARESISSDERG N
Sbjct: 181 SSMNCNKEGDNQHVAHGDDSLMNCNKEGDNQHVAHGDDSPSLGDVISARESISSDERGLN 240

Query: 241 FRYLDGTSGVLLEEDDSSRGLTNADASVLNTESSDSDSSEDPEVSQTFPSSDGREDNGPD 300
                  SGVLLEEDDSSRG+TNADASVLNTESSDSDSS DPEVS TFPSSDGRE+N PD
Sbjct: 241 -------SGVLLEEDDSSRGMTNADASVLNTESSDSDSSGDPEVSHTFPSSDGREENDPD 300

Query: 301 FTSKNSSPLVTEVTSKFRSNEDFTTWQRIIRLDAVRANAEWIVYAPTQAAVSDDKARHCA 360
           FTSKN SPLV EVTSKFRSNEDFTTWQRIIRLDAVRANAEWI YAP+QAAVSDDKARH A
Sbjct: 301 FTSKNLSPLV-EVTSKFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKARHLA 360

Query: 361 EVVGLKDYDHLESCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAF 420
           EVVGLKDYDHLES RIFHA+RLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAF
Sbjct: 361 EVVGLKDYDHLESSRIFHASRLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAF 420

Query: 421 WCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVV 480
           WCFVGFMRKARHNFRLDEVGIRKQLNIVSRII+FKDSHLYRHLQ+L+AEDCFFVYRMVVV
Sbjct: 421 WCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIRFKDSHLYRHLQNLEAEDCFFVYRMVVV 480

Query: 481 LFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKR 540
           LFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKR
Sbjct: 481 LFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKR 540

Query: 541 KLIIEKYNSMDEIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKIETS 570
           KLIIEKYNSMDEIIRECNSMAGQLDVWKLLD AHDLVVTLHEKIETS
Sbjct: 541 KLIIEKYNSMDEIIRECNSMAGQLDVWKLLDGAHDLVVTLHEKIETS 579

BLAST of Tan0008249 vs. NCBI nr
Match: XP_022968470.1 (small G protein signaling modulator 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 996.9 bits (2576), Expect = 7.2e-287
Identity = 531/606 (87.62%), Postives = 544/606 (89.77%), Query Frame = 0

Query: 1   MRALKRTPTPSSPDPNSNSPPSSSSSSSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLK 60
           MRALK+TPTPSSPDPNSNSPPSSSS SSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLK
Sbjct: 1   MRALKQTPTPSSPDPNSNSPPSSSSLSSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLK 60

Query: 61  SPWSRKKRKHALSLQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLGVYN 120
           SPWSRKKRKHALS QQWRSL+TPDGKLRDGG+KFLKKVRSGGVDPSIRAEVWPFLLGVY+
Sbjct: 61  SPWSRKKRKHALSPQQWRSLYTPDGKLRDGGLKFLKKVRSGGVDPSIRAEVWPFLLGVYD 120

Query: 121 LSSCEEERDAVRVQKRKEYERLRKQCRSLLKSGDESMKLNDDD----------------- 180
           L+S EEERDAVRVQKRKEYERLRKQCRSLLK GDESMKLNDDD                 
Sbjct: 121 LTSSEEERDAVRVQKRKEYERLRKQCRSLLKFGDESMKLNDDDMNCHKEGGIQHAAHGDG 180

Query: 181 --MNCNKERDNQHAAHGDDSPMNCNKEGDNQHVAHGD------------------DDSPS 240
             MNCNKE DNQH AHGDDS MNCNKEGDNQHVAHGD                  DDSPS
Sbjct: 181 SSMNCNKEGDNQHVAHGDDSSMNCNKEGDNQHVAHGDDSSMNCNKEGDNQHVAHGDDSPS 240

Query: 241 LEDVVSARESISSDERGSNFRYLDGTSGVLLEEDDSSRGLTNADASVLNTESSDSDSSED 300
           L DV+SARESISSDERG N       SGVLLEEDDSSRG+TNAD+SVLNTESSDSDSS D
Sbjct: 241 LGDVISARESISSDERGLN-------SGVLLEEDDSSRGMTNADSSVLNTESSDSDSSGD 300

Query: 301 PEVSQTFPSSDGREDNGPDFTSKNSSPLVTEVTSKFRSNEDFTTWQRIIRLDAVRANAEW 360
           PEVS TFPSSDGREDN PDFTSKN SPLV EVTSKFRSNEDFTTWQRIIRLDAVRANAEW
Sbjct: 301 PEVSHTFPSSDGREDNDPDFTSKNLSPLV-EVTSKFRSNEDFTTWQRIIRLDAVRANAEW 360

Query: 361 IVYAPTQAAVSDDKARHCAEVVGLKDYDHLESCRIFHAARLVTILEAYALYDPEIGYCQG 420
           I YAP+QAAVSDDKARH AEVVGLKDYDHLES RIFHA+RLVTILEAYALYDPEIGYCQG
Sbjct: 361 IAYAPSQAAVSDDKARHLAEVVGLKDYDHLESSRIFHASRLVTILEAYALYDPEIGYCQG 420

Query: 421 MSDLLSPIITVITEDHEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYR 480
           MSDLLSPIITVITEDHEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYR
Sbjct: 421 MSDLLSPIITVITEDHEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYR 480

Query: 481 HLQDLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRA 540
           HLQ+L+AEDCFFVYRMVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRA
Sbjct: 481 HLQNLEAEDCFFVYRMVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRA 540

Query: 541 PPTEDLLLYAIAASVLQKRKLIIEKYNSMDEIIRECNSMAGQLDVWKLLDDAHDLVVTLH 570
           PPTEDLLLYAIAASVLQKRKLIIE+YNSMDEIIRECNSMAGQLDVWKLLD AHDLVVTLH
Sbjct: 541 PPTEDLLLYAIAASVLQKRKLIIERYNSMDEIIRECNSMAGQLDVWKLLDGAHDLVVTLH 598

BLAST of Tan0008249 vs. NCBI nr
Match: XP_023541555.1 (small G protein signaling modulator 2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023541556.1 small G protein signaling modulator 2-like isoform X2 [Cucurbita pepo subsp. pepo] >XP_023541557.1 small G protein signaling modulator 2-like isoform X3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 993.4 bits (2567), Expect = 8.0e-286
Identity = 525/587 (89.44%), Postives = 539/587 (91.82%), Query Frame = 0

Query: 1   MRALKRTPTPSSPDPNSNSPPSSSSSSSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLK 60
           MRALK+TPTPSSPDPNSNSPP SSS SSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLK
Sbjct: 1   MRALKQTPTPSSPDPNSNSPPPSSSLSSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLK 60

Query: 61  SPWSRKKRKHALSLQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLGVYN 120
           SPWSRKKRKHALS QQWR L+TPDGKLRDGG+KFLKKVRSGGVDPSIRAEVWPFLLGVY+
Sbjct: 61  SPWSRKKRKHALSPQQWRGLYTPDGKLRDGGLKFLKKVRSGGVDPSIRAEVWPFLLGVYD 120

Query: 121 LSSCEEERDAVRVQKRKEYERLRKQCRSLLKSGDESMKLNDDDMNCNKERDNQHAAHGDD 180
           L+S EEERDAVRVQKRKEYERLRKQCRSLLK GDESMKLNDDDMNC+KE   QHAAHGDD
Sbjct: 121 LTSSEEERDAVRVQKRKEYERLRKQCRSLLKFGDESMKLNDDDMNCHKEGGIQHAAHGDD 180

Query: 181 SPMNCNKEGDNQHVAHGD------------------DDSPSLEDVVSARESISSDERGSN 240
           S MNCNKEGDNQHVA GD                  DDSPSL DV+SARESISSDERG N
Sbjct: 181 SSMNCNKEGDNQHVARGDDSSMNCNKEGDNQHVAHGDDSPSLGDVISARESISSDERGLN 240

Query: 241 FRYLDGTSGVLLEEDDSSRGLTNADASVLNTESSDSDSSEDPEVSQTFPSSDGREDNGPD 300
                  SGVLLEEDDSSRG+TNADASVLNTESSDSDSS DPEVS TFPSSDGREDN PD
Sbjct: 241 -------SGVLLEEDDSSRGMTNADASVLNTESSDSDSSGDPEVSHTFPSSDGREDNDPD 300

Query: 301 FTSKNSSPLVTEVTSKFRSNEDFTTWQRIIRLDAVRANAEWIVYAPTQAAVSDDKARHCA 360
           FTSKN SPLV EVTSKFRSNEDFTTWQRIIRLDAVRANAEWI YAP+QAAVSDDKARH A
Sbjct: 301 FTSKNLSPLV-EVTSKFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKARHLA 360

Query: 361 EVVGLKDYDHLESCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAF 420
           EVVGLKDYDHLE  RIFHA+RLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAF
Sbjct: 361 EVVGLKDYDHLEPSRIFHASRLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAF 420

Query: 421 WCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVV 480
           WCFVGFMRKARHNFRLDEVGIRKQLNIVSRII+FKDSHLYRHLQ+L+AEDCFFVYRMVVV
Sbjct: 421 WCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIRFKDSHLYRHLQNLEAEDCFFVYRMVVV 480

Query: 481 LFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKR 540
           LFRRELTFEQTLCLWEVMWADQAAIRAGVGKS+WSRIRQRAPPTEDLLLYAIAASVLQKR
Sbjct: 481 LFRRELTFEQTLCLWEVMWADQAAIRAGVGKSSWSRIRQRAPPTEDLLLYAIAASVLQKR 540

Query: 541 KLIIEKYNSMDEIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKIETS 570
           KLIIEKYNSMDEIIRECNSMAGQLDVWKLLD AHDLVVTLHEKIETS
Sbjct: 541 KLIIEKYNSMDEIIRECNSMAGQLDVWKLLDGAHDLVVTLHEKIETS 579

BLAST of Tan0008249 vs. NCBI nr
Match: XP_022945146.1 (small G protein signaling modulator 2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 993.4 bits (2567), Expect = 8.0e-286
Identity = 528/590 (89.49%), Postives = 542/590 (91.86%), Query Frame = 0

Query: 1   MRALKRTPTPSSPDPNSNSPPSSSSSSSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLK 60
           MRALK+TPTPSSPDPNSNSPPSSSS SSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLK
Sbjct: 1   MRALKQTPTPSSPDPNSNSPPSSSSLSSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLK 60

Query: 61  SPWSRKKRKHALSLQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLGVYN 120
           SPWSRKKRKHALS QQWRSL+TPDGKLRDGG+KFLKKVRSGGVDPSIRAEVWPFLLGVY+
Sbjct: 61  SPWSRKKRKHALSPQQWRSLYTPDGKLRDGGLKFLKKVRSGGVDPSIRAEVWPFLLGVYD 120

Query: 121 LSSCEEERDAVRVQKRKEYERLRKQCRSLLKSGDESMKLNDDDMNCNKERDNQHAAHGDD 180
           L+S EEERDAVRVQKRKEYERLRKQCRSLLK GDES+KLNDDDMNC+KE   QHAAHGDD
Sbjct: 121 LTSSEEERDAVRVQKRKEYERLRKQCRSLLKFGDESIKLNDDDMNCHKEGVIQHAAHGDD 180

Query: 181 SPMN---------------------CNKEGDNQHVAHGDDDSPSLEDVVSARESISSDER 240
           S MN                     CNKEGDNQHVAHG DDSPSL DV+SARESISSDER
Sbjct: 181 SSMNCCNKEGVIQHAAHGDDSSMNCCNKEGDNQHVAHG-DDSPSLGDVISARESISSDER 240

Query: 241 GSNFRYLDGTSGVLLEEDDSSRGLTNADASVLNTESSDSDSSEDPEVSQTFPSSDGREDN 300
           G N       SGVLLEEDDSSRG+TNADASVLNTESSDSDSS DPEVS TFPSSDGREDN
Sbjct: 241 GLN-------SGVLLEEDDSSRGMTNADASVLNTESSDSDSSGDPEVSHTFPSSDGREDN 300

Query: 301 GPDFTSKNSSPLVTEVTSKFRSNEDFTTWQRIIRLDAVRANAEWIVYAPTQAAVSDDKAR 360
            PDFTSKN SPLV EVTSKFRSNEDFTTWQRIIRLDAVRANAEWI YAP+QAAVSDDKAR
Sbjct: 301 DPDFTSKNLSPLV-EVTSKFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKAR 360

Query: 361 HCAEVVGLKDYDHLESCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDH 420
           H AEVVGLKDYDHLES RIFHA+RLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDH
Sbjct: 361 HLAEVVGLKDYDHLESSRIFHASRLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDH 420

Query: 421 EAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRM 480
           EAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRII+FKDSHLYRHLQ+L+AEDCFFVYRM
Sbjct: 421 EAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIRFKDSHLYRHLQNLEAEDCFFVYRM 480

Query: 481 VVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVL 540
           VVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVL
Sbjct: 481 VVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVL 540

Query: 541 QKRKLIIEKYNSMDEIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKIETS 570
           QKRKLIIEKYNSMDEIIRECNSMAGQLDVWKLLD AHDLVVTLHEKIETS
Sbjct: 541 QKRKLIIEKYNSMDEIIRECNSMAGQLDVWKLLDGAHDLVVTLHEKIETS 581

BLAST of Tan0008249 vs. ExPASy TrEMBL
Match: A0A6J1HTL1 (small G protein signaling modulator 2-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111467703 PE=4 SV=1)

HSP 1 Score: 1001.9 bits (2589), Expect = 1.1e-288
Identity = 530/588 (90.14%), Postives = 543/588 (92.35%), Query Frame = 0

Query: 1   MRALKRTPTPSSPDPNSNSPPSSSSSSSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLK 60
           MRALK+TPTPSSPDPNSNSPPSSSS SSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLK
Sbjct: 1   MRALKQTPTPSSPDPNSNSPPSSSSLSSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLK 60

Query: 61  SPWSRKKRKHALSLQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLGVYN 120
           SPWSRKKRKHALS QQWRSL+TPDGKLRDGG+KFLKKVRSGGVDPSIRAEVWPFLLGVY+
Sbjct: 61  SPWSRKKRKHALSPQQWRSLYTPDGKLRDGGLKFLKKVRSGGVDPSIRAEVWPFLLGVYD 120

Query: 121 LSSCEEERDAVRVQKRKEYERLRKQCRSLLKSGDESMKLNDDD----------------- 180
           L+S EEERDAVRVQKRKEYERLRKQCRSLLK GDESMKLNDDD                 
Sbjct: 121 LTSSEEERDAVRVQKRKEYERLRKQCRSLLKFGDESMKLNDDDMNCHKEGGIQHAAHGDG 180

Query: 181 --MNCNKERDNQHAAHGDDSPMNCNKEGDNQHVAHGDDDSPSLEDVVSARESISSDERGS 240
             MNCNKE DNQH AHGDDS MNCNKEGDNQHVAHG DDSPSL DV+SARESISSDERG 
Sbjct: 181 SSMNCNKEGDNQHVAHGDDSSMNCNKEGDNQHVAHG-DDSPSLGDVISARESISSDERGL 240

Query: 241 NFRYLDGTSGVLLEEDDSSRGLTNADASVLNTESSDSDSSEDPEVSQTFPSSDGREDNGP 300
           N       SGVLLEEDDSSRG+TNAD+SVLNTESSDSDSS DPEVS TFPSSDGREDN P
Sbjct: 241 N-------SGVLLEEDDSSRGMTNADSSVLNTESSDSDSSGDPEVSHTFPSSDGREDNDP 300

Query: 301 DFTSKNSSPLVTEVTSKFRSNEDFTTWQRIIRLDAVRANAEWIVYAPTQAAVSDDKARHC 360
           DFTSKN SPLV EVTSKFRSNEDFTTWQRIIRLDAVRANAEWI YAP+QAAVSDDKARH 
Sbjct: 301 DFTSKNLSPLV-EVTSKFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKARHL 360

Query: 361 AEVVGLKDYDHLESCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEA 420
           AEVVGLKDYDHLES RIFHA+RLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEA
Sbjct: 361 AEVVGLKDYDHLESSRIFHASRLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEA 420

Query: 421 FWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVV 480
           FWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQ+L+AEDCFFVYRMVV
Sbjct: 421 FWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQNLEAEDCFFVYRMVV 480

Query: 481 VLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQK 540
           VLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQK
Sbjct: 481 VLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQK 540

Query: 541 RKLIIEKYNSMDEIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKIETS 570
           RKLIIE+YNSMDEIIRECNSMAGQLDVWKLLD AHDLVVTLHEKIETS
Sbjct: 541 RKLIIERYNSMDEIIRECNSMAGQLDVWKLLDGAHDLVVTLHEKIETS 579

BLAST of Tan0008249 vs. ExPASy TrEMBL
Match: A0A6J1HX95 (small G protein signaling modulator 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111467703 PE=4 SV=1)

HSP 1 Score: 996.9 bits (2576), Expect = 3.5e-287
Identity = 531/606 (87.62%), Postives = 544/606 (89.77%), Query Frame = 0

Query: 1   MRALKRTPTPSSPDPNSNSPPSSSSSSSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLK 60
           MRALK+TPTPSSPDPNSNSPPSSSS SSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLK
Sbjct: 1   MRALKQTPTPSSPDPNSNSPPSSSSLSSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLK 60

Query: 61  SPWSRKKRKHALSLQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLGVYN 120
           SPWSRKKRKHALS QQWRSL+TPDGKLRDGG+KFLKKVRSGGVDPSIRAEVWPFLLGVY+
Sbjct: 61  SPWSRKKRKHALSPQQWRSLYTPDGKLRDGGLKFLKKVRSGGVDPSIRAEVWPFLLGVYD 120

Query: 121 LSSCEEERDAVRVQKRKEYERLRKQCRSLLKSGDESMKLNDDD----------------- 180
           L+S EEERDAVRVQKRKEYERLRKQCRSLLK GDESMKLNDDD                 
Sbjct: 121 LTSSEEERDAVRVQKRKEYERLRKQCRSLLKFGDESMKLNDDDMNCHKEGGIQHAAHGDG 180

Query: 181 --MNCNKERDNQHAAHGDDSPMNCNKEGDNQHVAHGD------------------DDSPS 240
             MNCNKE DNQH AHGDDS MNCNKEGDNQHVAHGD                  DDSPS
Sbjct: 181 SSMNCNKEGDNQHVAHGDDSSMNCNKEGDNQHVAHGDDSSMNCNKEGDNQHVAHGDDSPS 240

Query: 241 LEDVVSARESISSDERGSNFRYLDGTSGVLLEEDDSSRGLTNADASVLNTESSDSDSSED 300
           L DV+SARESISSDERG N       SGVLLEEDDSSRG+TNAD+SVLNTESSDSDSS D
Sbjct: 241 LGDVISARESISSDERGLN-------SGVLLEEDDSSRGMTNADSSVLNTESSDSDSSGD 300

Query: 301 PEVSQTFPSSDGREDNGPDFTSKNSSPLVTEVTSKFRSNEDFTTWQRIIRLDAVRANAEW 360
           PEVS TFPSSDGREDN PDFTSKN SPLV EVTSKFRSNEDFTTWQRIIRLDAVRANAEW
Sbjct: 301 PEVSHTFPSSDGREDNDPDFTSKNLSPLV-EVTSKFRSNEDFTTWQRIIRLDAVRANAEW 360

Query: 361 IVYAPTQAAVSDDKARHCAEVVGLKDYDHLESCRIFHAARLVTILEAYALYDPEIGYCQG 420
           I YAP+QAAVSDDKARH AEVVGLKDYDHLES RIFHA+RLVTILEAYALYDPEIGYCQG
Sbjct: 361 IAYAPSQAAVSDDKARHLAEVVGLKDYDHLESSRIFHASRLVTILEAYALYDPEIGYCQG 420

Query: 421 MSDLLSPIITVITEDHEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYR 480
           MSDLLSPIITVITEDHEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYR
Sbjct: 421 MSDLLSPIITVITEDHEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYR 480

Query: 481 HLQDLQAEDCFFVYRMVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRA 540
           HLQ+L+AEDCFFVYRMVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRA
Sbjct: 481 HLQNLEAEDCFFVYRMVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRA 540

Query: 541 PPTEDLLLYAIAASVLQKRKLIIEKYNSMDEIIRECNSMAGQLDVWKLLDDAHDLVVTLH 570
           PPTEDLLLYAIAASVLQKRKLIIE+YNSMDEIIRECNSMAGQLDVWKLLD AHDLVVTLH
Sbjct: 541 PPTEDLLLYAIAASVLQKRKLIIERYNSMDEIIRECNSMAGQLDVWKLLDGAHDLVVTLH 598

BLAST of Tan0008249 vs. ExPASy TrEMBL
Match: A0A6J1G016 (small G protein signaling modulator 2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449473 PE=4 SV=1)

HSP 1 Score: 993.4 bits (2567), Expect = 3.9e-286
Identity = 528/590 (89.49%), Postives = 542/590 (91.86%), Query Frame = 0

Query: 1   MRALKRTPTPSSPDPNSNSPPSSSSSSSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLK 60
           MRALK+TPTPSSPDPNSNSPPSSSS SSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLK
Sbjct: 1   MRALKQTPTPSSPDPNSNSPPSSSSLSSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLK 60

Query: 61  SPWSRKKRKHALSLQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLGVYN 120
           SPWSRKKRKHALS QQWRSL+TPDGKLRDGG+KFLKKVRSGGVDPSIRAEVWPFLLGVY+
Sbjct: 61  SPWSRKKRKHALSPQQWRSLYTPDGKLRDGGLKFLKKVRSGGVDPSIRAEVWPFLLGVYD 120

Query: 121 LSSCEEERDAVRVQKRKEYERLRKQCRSLLKSGDESMKLNDDDMNCNKERDNQHAAHGDD 180
           L+S EEERDAVRVQKRKEYERLRKQCRSLLK GDES+KLNDDDMNC+KE   QHAAHGDD
Sbjct: 121 LTSSEEERDAVRVQKRKEYERLRKQCRSLLKFGDESIKLNDDDMNCHKEGVIQHAAHGDD 180

Query: 181 SPMN---------------------CNKEGDNQHVAHGDDDSPSLEDVVSARESISSDER 240
           S MN                     CNKEGDNQHVAHG DDSPSL DV+SARESISSDER
Sbjct: 181 SSMNCCNKEGVIQHAAHGDDSSMNCCNKEGDNQHVAHG-DDSPSLGDVISARESISSDER 240

Query: 241 GSNFRYLDGTSGVLLEEDDSSRGLTNADASVLNTESSDSDSSEDPEVSQTFPSSDGREDN 300
           G N       SGVLLEEDDSSRG+TNADASVLNTESSDSDSS DPEVS TFPSSDGREDN
Sbjct: 241 GLN-------SGVLLEEDDSSRGMTNADASVLNTESSDSDSSGDPEVSHTFPSSDGREDN 300

Query: 301 GPDFTSKNSSPLVTEVTSKFRSNEDFTTWQRIIRLDAVRANAEWIVYAPTQAAVSDDKAR 360
            PDFTSKN SPLV EVTSKFRSNEDFTTWQRIIRLDAVRANAEWI YAP+QAAVSDDKAR
Sbjct: 301 DPDFTSKNLSPLV-EVTSKFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKAR 360

Query: 361 HCAEVVGLKDYDHLESCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDH 420
           H AEVVGLKDYDHLES RIFHA+RLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDH
Sbjct: 361 HLAEVVGLKDYDHLESSRIFHASRLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDH 420

Query: 421 EAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRM 480
           EAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRII+FKDSHLYRHLQ+L+AEDCFFVYRM
Sbjct: 421 EAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIRFKDSHLYRHLQNLEAEDCFFVYRM 480

Query: 481 VVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVL 540
           VVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVL
Sbjct: 481 VVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVL 540

Query: 541 QKRKLIIEKYNSMDEIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKIETS 570
           QKRKLIIEKYNSMDEIIRECNSMAGQLDVWKLLD AHDLVVTLHEKIETS
Sbjct: 541 QKRKLIIEKYNSMDEIIRECNSMAGQLDVWKLLDGAHDLVVTLHEKIETS 581

BLAST of Tan0008249 vs. ExPASy TrEMBL
Match: A0A6J1CHH5 (GTPase-activating protein GYP7-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111011552 PE=4 SV=1)

HSP 1 Score: 991.9 bits (2563), Expect = 1.1e-285
Identity = 526/584 (90.07%), Postives = 539/584 (92.29%), Query Frame = 0

Query: 1   MRALKRTPTPSSPDPNSNSPPSSSSSSSA------------SSSWVHLRSVLFVVTSSSP 60
           MRALKRT TPSSPDPNSNSPPSSSSSSSA            SSSWVHLRSVLFVVTSSSP
Sbjct: 1   MRALKRTQTPSSPDPNSNSPPSSSSSSSASSSAPSSSSSSSSSSWVHLRSVLFVVTSSSP 60

Query: 61  ASSSSSDRGRLKSPWSRKKRKHALSLQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIR 120
           ASSSSSDRGRLKSPWSRKKRK+AL+ QQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIR
Sbjct: 61  ASSSSSDRGRLKSPWSRKKRKNALTPQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIR 120

Query: 121 AEVWPFLLGVYNLSSCEEERDAVRVQKRKEYERLRKQCRSLLKSGDESMKLNDDDMNCNK 180
           AEVWPFLLGVY+LSS EEERDAVR+QKRKEYERLRKQCR LLKSGDES KLNDDDMN NK
Sbjct: 121 AEVWPFLLGVYDLSSSEEERDAVRLQKRKEYERLRKQCRGLLKSGDESTKLNDDDMNSNK 180

Query: 181 ERDNQHAAHGDDSPMNCNKEGDNQHVAHGDDDSPSLEDVVSARESISSDERGSNFRYLDG 240
           ERD       +D   N NKEGDNQ+VAHG DD PSLE+VVSARESISSDERGSNFR LDG
Sbjct: 181 ERDENIKL--NDGETNYNKEGDNQYVAHG-DDCPSLEEVVSARESISSDERGSNFRNLDG 240

Query: 241 TSGVLLEEDDSSRGLTNADASVLNTESSDSDSSEDPEVSQTFPSSDGREDNGPDFTSKNS 300
           TSGV LEEDDSSR +T+ADAS  NTESSDSDSSEDPEVSQTFPSSDGREDN PDFTSKNS
Sbjct: 241 TSGVFLEEDDSSRRMTSADASAPNTESSDSDSSEDPEVSQTFPSSDGREDNDPDFTSKNS 300

Query: 301 SPLVTEVTSKFRSNEDFTTWQRIIRLDAVRANAEWIVYAPTQAAVSDDKARHCAEVVGLK 360
           SP V EVTSKFRSNEDFTTWQRIIRLDAVRAN+EWI YAP+QAAVSDDKARH AEVVGLK
Sbjct: 301 SPSVAEVTSKFRSNEDFTTWQRIIRLDAVRANSEWIAYAPSQAAVSDDKARHSAEVVGLK 360

Query: 361 DYDHLESCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGF 420
           DYDHLE  RIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITED EAFWCFVGF
Sbjct: 361 DYDHLEPSRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDQEAFWCFVGF 420

Query: 421 MRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVVLFRREL 480
           MRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHL+ LQAEDCFFVYRMVVVLFRREL
Sbjct: 421 MRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLEKLQAEDCFFVYRMVVVLFRREL 480

Query: 481 TFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEK 540
           TFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEK
Sbjct: 481 TFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEK 540

Query: 541 YNSMDEIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKIETSFNE 573
           YNSMDEIIRECNSMAGQLD+WKLLDDAHDLVVTLHEKIETSFNE
Sbjct: 541 YNSMDEIIRECNSMAGQLDIWKLLDDAHDLVVTLHEKIETSFNE 581

BLAST of Tan0008249 vs. ExPASy TrEMBL
Match: A0A6J1G038 (small G protein signaling modulator 2-like isoform X4 OS=Cucurbita moschata OX=3662 GN=LOC111449473 PE=4 SV=1)

HSP 1 Score: 991.1 bits (2561), Expect = 1.9e-285
Identity = 527/589 (89.47%), Postives = 541/589 (91.85%), Query Frame = 0

Query: 2   RALKRTPTPSSPDPNSNSPPSSSSSSSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLKS 61
           RALK+TPTPSSPDPNSNSPPSSSS SSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLKS
Sbjct: 52  RALKQTPTPSSPDPNSNSPPSSSSLSSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLKS 111

Query: 62  PWSRKKRKHALSLQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLGVYNL 121
           PWSRKKRKHALS QQWRSL+TPDGKLRDGG+KFLKKVRSGGVDPSIRAEVWPFLLGVY+L
Sbjct: 112 PWSRKKRKHALSPQQWRSLYTPDGKLRDGGLKFLKKVRSGGVDPSIRAEVWPFLLGVYDL 171

Query: 122 SSCEEERDAVRVQKRKEYERLRKQCRSLLKSGDESMKLNDDDMNCNKERDNQHAAHGDDS 181
           +S EEERDAVRVQKRKEYERLRKQCRSLLK GDES+KLNDDDMNC+KE   QHAAHGDDS
Sbjct: 172 TSSEEERDAVRVQKRKEYERLRKQCRSLLKFGDESIKLNDDDMNCHKEGVIQHAAHGDDS 231

Query: 182 PMN---------------------CNKEGDNQHVAHGDDDSPSLEDVVSARESISSDERG 241
            MN                     CNKEGDNQHVAHG DDSPSL DV+SARESISSDERG
Sbjct: 232 SMNCCNKEGVIQHAAHGDDSSMNCCNKEGDNQHVAHG-DDSPSLGDVISARESISSDERG 291

Query: 242 SNFRYLDGTSGVLLEEDDSSRGLTNADASVLNTESSDSDSSEDPEVSQTFPSSDGREDNG 301
            N       SGVLLEEDDSSRG+TNADASVLNTESSDSDSS DPEVS TFPSSDGREDN 
Sbjct: 292 LN-------SGVLLEEDDSSRGMTNADASVLNTESSDSDSSGDPEVSHTFPSSDGREDND 351

Query: 302 PDFTSKNSSPLVTEVTSKFRSNEDFTTWQRIIRLDAVRANAEWIVYAPTQAAVSDDKARH 361
           PDFTSKN SPLV EVTSKFRSNEDFTTWQRIIRLDAVRANAEWI YAP+QAAVSDDKARH
Sbjct: 352 PDFTSKNLSPLV-EVTSKFRSNEDFTTWQRIIRLDAVRANAEWIAYAPSQAAVSDDKARH 411

Query: 362 CAEVVGLKDYDHLESCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHE 421
            AEVVGLKDYDHLES RIFHA+RLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHE
Sbjct: 412 LAEVVGLKDYDHLESSRIFHASRLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHE 471

Query: 422 AFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMV 481
           AFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRII+FKDSHLYRHLQ+L+AEDCFFVYRMV
Sbjct: 472 AFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIRFKDSHLYRHLQNLEAEDCFFVYRMV 531

Query: 482 VVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQ 541
           VVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQ
Sbjct: 532 VVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQ 591

Query: 542 KRKLIIEKYNSMDEIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKIETS 570
           KRKLIIEKYNSMDEIIRECNSMAGQLDVWKLLD AHDLVVTLHEKIETS
Sbjct: 592 KRKLIIEKYNSMDEIIRECNSMAGQLDVWKLLDGAHDLVVTLHEKIETS 631

BLAST of Tan0008249 vs. TAIR 10
Match: AT3G49350.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 656.8 bits (1693), Expect = 1.6e-188
Identity = 363/573 (63.35%), Postives = 429/573 (74.87%), Query Frame = 0

Query: 1   MRALKRTPTPSSPDPNSNSPPSSSSSSSASSSWVHLRSVLFVVTSSSPASSSSSDRGRLK 60
           M+AL+R  T SS   N +SP SS  SSS+SSSW+ +RS LFVV SSSPA  S SDR RLK
Sbjct: 1   MKALRRIQTTSS-SSNPSSPLSSPPSSSSSSSWIQIRSALFVVASSSPA--SCSDRPRLK 60

Query: 61  SPWSRKKRKHALSLQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLGVYN 120
           SPWSR+KRK  L  QQW+  FTPDG+LR+GG+  LKKVRS G++PSIR EVWPFLLG+Y 
Sbjct: 61  SPWSRRKRKRPLRPQQWKRFFTPDGRLRNGGVDLLKKVRSRGIEPSIRLEVWPFLLGLYG 120

Query: 121 LSSCEEERDAVRVQKRKEYERLRKQCRSLLKSGDESMKLNDDDMNCNKERDNQHAAHGDD 180
            +S +EER  +R ++RKEYERLR+QC+ L K  + + KL +       E D         
Sbjct: 121 FNSSKEERVTIRNRRRKEYERLRRQCKRLQKHNNGTRKLYNGSETIQDEYDWPRVQ---- 180

Query: 181 SPMNCNKEGDNQHVAHGDDDSPSLEDVVSARESISSDER-GSNFRYLDGTSGVLLEED-D 240
                            D DS   ++VVSARES+SSDE    +  Y+   S  +  +D  
Sbjct: 181 -----------------DTDSSCSDEVVSARESLSSDEDITEDIGYMSEVSCTVERDDGS 240

Query: 241 SSRGLTNADASVLNTESSDSDSSEDPEVSQTFPSSDGREDNGPDFTSKNSSPLVTEVTSK 300
           SSR +TNA  S LN+ESSDSDSS++ EV Q F SS    D    + + +S P        
Sbjct: 241 SSRRITNATISTLNSESSDSDSSDESEVVQVFQSS-ATPDVNSTYPASSSIP-------- 300

Query: 301 FRSNEDFTTWQRIIRLDAVRANAEWIVYAPTQAAVSDDKARHCAEVVGLKDYDHLESCRI 360
            R+ EDF TWQRIIRLDAVRA++EW  Y+P+QA +S+D+A   AE VGLKDY+HLE  +I
Sbjct: 301 -RTEEDFVTWQRIIRLDAVRADSEWTSYSPSQAVISEDRACRAAEAVGLKDYNHLEPYKI 360

Query: 361 FHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARHNFRL 420
           F AARLV +LEAYALYDP+IGYCQGMSDLLSPI++VI +DHE FWCFVGFM+KARHNFRL
Sbjct: 361 FQAARLVAVLEAYALYDPDIGYCQGMSDLLSPILSVIPDDHEVFWCFVGFMKKARHNFRL 420

Query: 421 DEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVVLFRRELTFEQTLCLWE 480
           DEVGIR+QLNIVS+IIK KDS LYRHL+ LQAEDCFFVYRMVVV+FRRELT +QTLCLWE
Sbjct: 421 DEVGIRRQLNIVSKIIKSKDSQLYRHLEKLQAEDCFFVYRMVVVMFRRELTLDQTLCLWE 480

Query: 481 VMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSMDEIIRE 540
           VMWADQAAIRAG+GKSAWSRIRQRAPPT+DL+LYAIAASVLQ+RK IIE+YNSMDEI+RE
Sbjct: 481 VMWADQAAIRAGMGKSAWSRIRQRAPPTDDLVLYAIAASVLQRRKRIIERYNSMDEILRE 539

Query: 541 CNSMAGQLDVWKLLDDAHDLVVTLHEKIETSFN 572
           C SMAGQLDVWKLLDDAHDLVVTLH KIE SF+
Sbjct: 541 CQSMAGQLDVWKLLDDAHDLVVTLHTKIEHSFS 539

BLAST of Tan0008249 vs. TAIR 10
Match: AT5G53570.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 623.2 bits (1606), Expect = 2.0e-178
Identity = 353/582 (60.65%), Postives = 426/582 (73.20%), Query Frame = 0

Query: 3   ALKRTPTPSSPDPNSNSPPSS-------SSSSSASSSWVHLRSVLFVVTSSSPASSSSSD 62
           +L  + + S P  +S+SPPSS       S+SSS+SSSW+HLRSVLFV   SSP+S +SSD
Sbjct: 21  SLPSSSSSSLPSSSSSSPPSSNSNSYSNSNSSSSSSSWIHLRSVLFVANLSSPSSVTSSD 80

Query: 63  RGRLKSPWSRKKRKHALSLQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFL 122
           R R KSPWSR+KRK AL+  QWRSLFTP+GKLRDGG+ FLKKVRS GVDPSIRAEVW FL
Sbjct: 81  R-RRKSPWSRRKRKWALTPHQWRSLFTPEGKLRDGGVGFLKKVRSRGVDPSIRAEVWLFL 140

Query: 123 LGVYNLSSCEEERDAVRVQKRKEYERLRKQCRSLLKSGDESMKLNDDDMNCNKERDNQHA 182
           LGVY+L+S  EER+AV+ QKRKEYE+L+++C+ LLK G             N   DN   
Sbjct: 141 LGVYDLNSTSEEREAVKTQKRKEYEKLQRRCQMLLKCG-------------NGSTDNLEE 200

Query: 183 AHGDDSPMNCNKEGDNQHVAHGDDDSPSLEDVVSARESISSDERGSNFRYLDGTSGVLLE 242
              D++   C +  D+  +        + +DVVSA                         
Sbjct: 201 LPSDEANSQCVRFVDDYKIT----GPMTSQDVVSA------------------------- 260

Query: 243 EDDSSRGLTNADASVLNTESSDSDSSEDPE---VSQTFPSSDGR---EDNGPDFTSKNSS 302
                          LNT+SSD+DS ED E   +  +F  SD +   EDN  + + +NSS
Sbjct: 261 ---------------LNTDSSDTDSCEDNEDVLLLSSFAHSDEKKPEEDNSNNNSEENSS 320

Query: 303 PLV---TEVTSKFRSNEDFTTWQRIIRLDAVRANAEWIVYAPTQAAVSDDKARHCAEVVG 362
            LV   +EV  +   +EDF+TWQRIIRLDA+RA++EW  Y+P   A+++ KAR  AE VG
Sbjct: 321 LLVAAASEVQVEVAVHEDFSTWQRIIRLDALRADSEWANYSPYSTAITESKARRLAESVG 380

Query: 363 LKDYDHLESCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFV 422
           LKDYDHLESCR++HAARLV ILEAYA+YDPEIGYCQGMSDLLSPI+ VI+EDHEAFWCFV
Sbjct: 381 LKDYDHLESCRLYHAARLVAILEAYAMYDPEIGYCQGMSDLLSPILAVISEDHEAFWCFV 440

Query: 423 GFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVVLFRR 482
           GFM+KARHNFRLDE GI++QL+IVS+IIK KDS LY+HL++LQAEDC FVYRMV+V+FRR
Sbjct: 441 GFMKKARHNFRLDEAGIQRQLSIVSKIIKNKDSQLYKHLENLQAEDCSFVYRMVLVMFRR 500

Query: 483 ELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLII 542
           EL+FEQTLCLWEVMWADQAAIRAGVGKS WSRIRQ+APPT+DLLLYAIAA VL +RKLII
Sbjct: 501 ELSFEQTLCLWEVMWADQAAIRAGVGKSPWSRIRQQAPPTDDLLLYAIAALVL-RRKLII 543

Query: 543 EKYNSMDEIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKIET 569
           +KY+SMDEI+ ECNSMAGQL+VWKLLDDAH LVVTLH+KIET
Sbjct: 561 QKYSSMDEIVEECNSMAGQLNVWKLLDDAHHLVVTLHDKIET 543

BLAST of Tan0008249 vs. TAIR 10
Match: AT5G53570.2 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 623.2 bits (1606), Expect = 2.0e-178
Identity = 353/582 (60.65%), Postives = 426/582 (73.20%), Query Frame = 0

Query: 3   ALKRTPTPSSPDPNSNSPPSS-------SSSSSASSSWVHLRSVLFVVTSSSPASSSSSD 62
           +L  + + S P  +S+SPPSS       S+SSS+SSSW+HLRSVLFV   SSP+S +SSD
Sbjct: 48  SLPSSSSSSLPSSSSSSPPSSNSNSYSNSNSSSSSSSWIHLRSVLFVANLSSPSSVTSSD 107

Query: 63  RGRLKSPWSRKKRKHALSLQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFL 122
           R R KSPWSR+KRK AL+  QWRSLFTP+GKLRDGG+ FLKKVRS GVDPSIRAEVW FL
Sbjct: 108 R-RRKSPWSRRKRKWALTPHQWRSLFTPEGKLRDGGVGFLKKVRSRGVDPSIRAEVWLFL 167

Query: 123 LGVYNLSSCEEERDAVRVQKRKEYERLRKQCRSLLKSGDESMKLNDDDMNCNKERDNQHA 182
           LGVY+L+S  EER+AV+ QKRKEYE+L+++C+ LLK G             N   DN   
Sbjct: 168 LGVYDLNSTSEEREAVKTQKRKEYEKLQRRCQMLLKCG-------------NGSTDNLEE 227

Query: 183 AHGDDSPMNCNKEGDNQHVAHGDDDSPSLEDVVSARESISSDERGSNFRYLDGTSGVLLE 242
              D++   C +  D+  +        + +DVVSA                         
Sbjct: 228 LPSDEANSQCVRFVDDYKIT----GPMTSQDVVSA------------------------- 287

Query: 243 EDDSSRGLTNADASVLNTESSDSDSSEDPE---VSQTFPSSDGR---EDNGPDFTSKNSS 302
                          LNT+SSD+DS ED E   +  +F  SD +   EDN  + + +NSS
Sbjct: 288 ---------------LNTDSSDTDSCEDNEDVLLLSSFAHSDEKKPEEDNSNNNSEENSS 347

Query: 303 PLV---TEVTSKFRSNEDFTTWQRIIRLDAVRANAEWIVYAPTQAAVSDDKARHCAEVVG 362
            LV   +EV  +   +EDF+TWQRIIRLDA+RA++EW  Y+P   A+++ KAR  AE VG
Sbjct: 348 LLVAAASEVQVEVAVHEDFSTWQRIIRLDALRADSEWANYSPYSTAITESKARRLAESVG 407

Query: 363 LKDYDHLESCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFV 422
           LKDYDHLESCR++HAARLV ILEAYA+YDPEIGYCQGMSDLLSPI+ VI+EDHEAFWCFV
Sbjct: 408 LKDYDHLESCRLYHAARLVAILEAYAMYDPEIGYCQGMSDLLSPILAVISEDHEAFWCFV 467

Query: 423 GFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVVLFRR 482
           GFM+KARHNFRLDE GI++QL+IVS+IIK KDS LY+HL++LQAEDC FVYRMV+V+FRR
Sbjct: 468 GFMKKARHNFRLDEAGIQRQLSIVSKIIKNKDSQLYKHLENLQAEDCSFVYRMVLVMFRR 527

Query: 483 ELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAASVLQKRKLII 542
           EL+FEQTLCLWEVMWADQAAIRAGVGKS WSRIRQ+APPT+DLLLYAIAA VL +RKLII
Sbjct: 528 ELSFEQTLCLWEVMWADQAAIRAGVGKSPWSRIRQQAPPTDDLLLYAIAALVL-RRKLII 570

Query: 543 EKYNSMDEIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKIET 569
           +KY+SMDEI+ ECNSMAGQL+VWKLLDDAH LVVTLH+KIET
Sbjct: 588 QKYSSMDEIVEECNSMAGQLNVWKLLDDAHHLVVTLHDKIET 570

BLAST of Tan0008249 vs. TAIR 10
Match: AT5G24390.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 591.3 bits (1523), Expect = 8.5e-169
Identity = 332/573 (57.94%), Postives = 420/573 (73.30%), Query Frame = 0

Query: 1   MRALKRTPTPSSPDPNSNSPPSSSSSSSASSSWVHLRSVLFVVTSSSPAS-SSSSDRGRL 60
           M+AL+R  T S+  PN +SP         SS W HLRS   +VTSSSPAS SSSSD  RL
Sbjct: 1   MKALRRIQTKST-FPNPSSP---------SSPWTHLRSAFVLVTSSSPASCSSSSDPHRL 60

Query: 61  KSPWSRKKRKHALSLQQWRSLFTPDGKLRDGGIKFLKKVRSGGVDPSIRAEVWPFLLGVY 120
           KSPWSR+K K  L+L++WR  FTP+G++R+GG+  LKKVR+ G+DPSIR+EVWPFLLGV 
Sbjct: 61  KSPWSRRKGKKPLTLRRWRRCFTPEGRIRNGGVDLLKKVRTRGIDPSIRSEVWPFLLGVC 120

Query: 121 NLSSCEEERDAVRVQKRKEYERLRKQCRSLLKSGDESMKLNDDDMNCNK-ERDNQHAAHG 180
           + +S EEER A R  +RK YERLR+QC+ L K    + KLN      NK  +D++H    
Sbjct: 121 DFNSSEEERGATRTWRRKVYERLRRQCKRLQKQNSGTFKLN----KINKTTQDDEH---- 180

Query: 181 DDSPMNCNKEGDNQHVAHGDDDSPSLEDVVSARESISSD-ERGSNFRYLDGTSGVLLEED 240
                      D+  +A  D +S   +D  S  ES+ SD +   +  Y+   S  L  + 
Sbjct: 181 -----------DSWSLAQ-DSESSCSDDACSVHESLISDKDNTEDIGYMSDVSCTLDRDY 240

Query: 241 DSSRGLTNADASVLNTESSDSDSSEDPEVSQTFPSSDGREDNGPDFTSKNSSPLVTEVTS 300
           + SR         LN+ESSDSDSS++ +  Q  PS +G+++N       ++SP    ++ 
Sbjct: 241 NGSRH--------LNSESSDSDSSDENDYVQVAPSCEGKDEN-------STSPSSIYIS- 300

Query: 301 KFRSNEDFTTWQRIIRLDAVRANAEWIVYAPTQAAVSDDKARHCAEVVGLKDYDHLESCR 360
             R+ EDF TWQRIIRLDA+RA+ EW  Y+ +QA VS+++AR  AE VGLKDY +LE  +
Sbjct: 301 --RTKEDFVTWQRIIRLDALRADTEWTPYSQSQALVSENRARRAAEAVGLKDYSYLEPSK 360

Query: 361 IFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVITEDHEAFWCFVGFMRKARHNFR 420
           IFHAARLV +LEAYAL+DPEIGYCQGMSDLLSPI++VI +D+EAFWCFVGFM+KAR NFR
Sbjct: 361 IFHAARLVAVLEAYALHDPEIGYCQGMSDLLSPILSVIPDDYEAFWCFVGFMKKARQNFR 420

Query: 421 LDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFVYRMVVVLFRRELTFEQTLCLW 480
           +DEVGI +QLNIVS+IIK KDS LY+HL+ ++AEDCFFVYRMV+V+FRRELT EQTL LW
Sbjct: 421 VDEVGITRQLNIVSKIIKSKDSQLYKHLEKVKAEDCFFVYRMVLVMFRRELTLEQTLHLW 480

Query: 481 EVMWADQAAIRAGVGKSAWS-RIRQRAPPTEDLLLYAIAASVLQKRKLIIEKYNSMDEII 540
           EV+WADQAAIRAG+GKS+WS RI+QRAPPTEDLLLY +AASVLQ+RK+IIEKY+SM+EI+
Sbjct: 481 EVIWADQAAIRAGMGKSSWSRRIKQRAPPTEDLLLYVVAASVLQRRKVIIEKYSSMEEIL 525

Query: 541 RECNSMAGQLDVWKLLDDAHDLVVTLHEKIETS 570
           REC++M G+LDVWKLLDDAHDL+VTLH KIE S
Sbjct: 541 RECHNMVGKLDVWKLLDDAHDLIVTLHAKIEHS 525

BLAST of Tan0008249 vs. TAIR 10
Match: AT5G41940.1 (Ypt/Rab-GAP domain of gyp1p superfamily protein )

HSP 1 Score: 550.8 bits (1418), Expect = 1.3e-156
Identity = 304/530 (57.36%), Postives = 376/530 (70.94%), Query Frame = 0

Query: 38  SVLFVVTSSSPASSSSSDRGRLKSPWSRKKRKHALSLQQWRSLFTPDGKLRDGGIKFLKK 97
           +V   V + + A +  S RG + SPWS ++RK  L  +QW + FT +G+L DGG+KFLKK
Sbjct: 55  AVAVTVMAVAVAFTVYSRRGSIGSPWSLRRRKRVLQPKQWNAFFTEEGRLSDGGVKFLKK 114

Query: 98  VRSGGVDPSIRAEVWPFLLGVYNLSSCEEERDAVRVQKRKEYERLRKQCRSLLKSGDESM 157
           VRSGGV PSIR EVWPFLLGVY+L S +EERD++R  K  EYE LR+QCR          
Sbjct: 115 VRSGGVHPSIRPEVWPFLLGVYDLKSNKEERDSIRQLKLTEYENLRRQCR---------- 174

Query: 158 KLNDDDMNCNKERDNQHAAHGDDSPMNCNKEGDNQHVAHGDDDSPSLEDVVSARESISSD 217
           ++++   N    ++   +++ +DS +                +S  +E+V S+  +I+ D
Sbjct: 175 EIHERYENVCDSKETAQSSNTEDSQVL---------------ESHDIEEVESSTRTITVD 234

Query: 218 ERGSNFRYLDGTSGVLLEEDDSSRGLTNADASVLNTESSDSDSSEDPEVSQTFPSSDGRE 277
           E          +  ++ +E+     +T  DA+      SDS + E+ E S    + +   
Sbjct: 235 ESEKL-----NSESIMQDENCEKSDVTTEDAA---GNDSDSTNPEETETSPFLANEEVES 294

Query: 278 DNGPDFTSKNSSPLVTEVTSKFRSNEDF-TTWQRIIRLDAVRANAEWIVYAPTQAAVSDD 337
            N  +   + SSP  +       ++EDF +TWQRIIRLDAVRAN EW+ Y+PTQAAVS+ 
Sbjct: 295 HNTVNQEKEISSP--SSKPKSQAADEDFMSTWQRIIRLDAVRANDEWVPYSPTQAAVSET 354

Query: 338 KARHCAEVVGLKDYDHLESCRIFHAARLVTILEAYALYDPEIGYCQGMSDLLSPIITVIT 397
           KAR  A  VGL DYDHLE CRIFHAARLV ILEAYA+YDPEIGYCQGMSDLLSP+I V+ 
Sbjct: 355 KARGIAIQVGLNDYDHLEPCRIFHAARLVGILEAYAVYDPEIGYCQGMSDLLSPLIAVME 414

Query: 398 EDHEAFWCFVGFMRKARHNFRLDEVGIRKQLNIVSRIIKFKDSHLYRHLQDLQAEDCFFV 457
           +D  AFWCFVGFM KARHNFRLDEVGIR+QL++VS+IIKFKD HLYRHL++L+AEDCFFV
Sbjct: 415 DDVLAFWCFVGFMSKARHNFRLDEVGIRRQLSMVSKIIKFKDIHLYRHLENLEAEDCFFV 474

Query: 458 YRMVVVLFRRELTFEQTLCLWEVMWADQAAIRAGVGKSAWSRIRQRAPPTEDLLLYAIAA 517
           YRMVVVLFRRELTFEQTLCLWEVMWADQAAIR G+ K+ W RIR RAPPTEDLLLYAIAA
Sbjct: 475 YRMVVVLFRRELTFEQTLCLWEVMWADQAAIRTGIAKATWGRIRLRAPPTEDLLLYAIAA 534

Query: 518 SVLQKRKLIIEKYNSMDEIIRECNSMAGQLDVWKLLDDAHDLVVTLHEKI 567
           SVLQ+RK IIEKY+ MDEI++ECNSMAG LDVWKLLDDAHDLVV LH+KI
Sbjct: 535 SVLQRRKTIIEKYSGMDEIMKECNSMAGHLDVWKLLDDAHDLVVNLHDKI 549

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94BY92.8e-17760.65Rab GTPase-activating protein 22 OS=Arabidopsis thaliana OX=3702 GN=RABGAP22 PE=... [more]
P093791.7e-2530.29GTPase-activating protein GYP7 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) ... [more]
Q8TC071.1e-2230.65TBC1 domain family member 15 OS=Homo sapiens OX=9606 GN=TBC1D15 PE=1 SV=2[more]
Q6FWI14.0e-2228.57GTPase-activating protein GYP7 OS=Candida glabrata (strain ATCC 2001 / CBS 138 /... [more]
Q9UUH78.9e-2230.56GTPase-activating protein gyp7 OS=Schizosaccharomyces pombe (strain 972 / ATCC 2... [more]
Match NameE-valueIdentityDescription
XP_022968472.12.2e-28890.14small G protein signaling modulator 2-like isoform X3 [Cucurbita maxima][more]
KAG7013330.12.9e-28890.12GYP7 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022968470.17.2e-28787.62small G protein signaling modulator 1-like isoform X1 [Cucurbita maxima][more]
XP_023541555.18.0e-28689.44small G protein signaling modulator 2-like isoform X1 [Cucurbita pepo subsp. pep... [more]
XP_022945146.18.0e-28689.49small G protein signaling modulator 2-like isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1HTL11.1e-28890.14small G protein signaling modulator 2-like isoform X3 OS=Cucurbita maxima OX=366... [more]
A0A6J1HX953.5e-28787.62small G protein signaling modulator 1-like isoform X1 OS=Cucurbita maxima OX=366... [more]
A0A6J1G0163.9e-28689.49small G protein signaling modulator 2-like isoform X1 OS=Cucurbita moschata OX=3... [more]
A0A6J1CHH51.1e-28590.07GTPase-activating protein GYP7-like isoform X1 OS=Momordica charantia OX=3673 GN... [more]
A0A6J1G0381.9e-28589.47small G protein signaling modulator 2-like isoform X4 OS=Cucurbita moschata OX=3... [more]
Match NameE-valueIdentityDescription
AT3G49350.11.6e-18863.35Ypt/Rab-GAP domain of gyp1p superfamily protein [more]
AT5G53570.12.0e-17860.65Ypt/Rab-GAP domain of gyp1p superfamily protein [more]
AT5G53570.22.0e-17860.65Ypt/Rab-GAP domain of gyp1p superfamily protein [more]
AT5G24390.18.5e-16957.94Ypt/Rab-GAP domain of gyp1p superfamily protein [more]
AT5G41940.11.3e-15657.36Ypt/Rab-GAP domain of gyp1p superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 128..148
NoneNo IPR availableGENE3D1.10.472.80coord: 416..563
e-value: 7.6E-22
score: 79.5
NoneNo IPR availableGENE3D1.10.8.270putative rabgap domain of human tbc1 domain family member 14 like domainscoord: 282..412
e-value: 5.5E-18
score: 67.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 150..218
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 239..271
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 232..287
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 150..202
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 204..218
NoneNo IPR availablePANTHERPTHR22957TBC1 DOMAIN FAMILY MEMBER GTPASE-ACTIVATING PROTEINcoord: 21..562
NoneNo IPR availablePANTHERPTHR22957:SF580SMALL G PROTEIN SIGNALING MODULATOR 2-LIKE ISOFORM X1coord: 21..562
IPR000195Rab-GTPase-TBC domainSMARTSM00164tbc_4coord: 95..525
e-value: 4.0E-30
score: 116.1
IPR000195Rab-GTPase-TBC domainPFAMPF00566RabGAP-TBCcoord: 359..480
e-value: 1.0E-31
score: 110.3
IPR000195Rab-GTPase-TBC domainPROSITEPS50086TBC_RABGAPcoord: 98..480
score: 31.321386
IPR035969Rab-GTPase-TBC domain superfamilySUPERFAMILY47923Ypt/Rab-GAP domain of gyp1pcoord: 417..554
IPR035969Rab-GTPase-TBC domain superfamilySUPERFAMILY47923Ypt/Rab-GAP domain of gyp1pcoord: 80..440

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0008249.1Tan0008249.1mRNA
Tan0008249.2Tan0008249.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090630 activation of GTPase activity
biological_process GO:0006886 intracellular protein transport
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005096 GTPase activator activity