Tan0008221 (gene) Snake gourd v1

BLAST of Tan0008221 vs. ExPASy Swiss-Prot
Match: Q8BJM3 (Coiled-coil domain-containing protein R3HCC1L OS=Mus musculus OX=10090 GN=R3hcc1l PE=1 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 2.3e-13
Identity = 48/143 (33.57%), Postives = 76/143 (53.15%), Query Frame = 0

Query: 236 HVIVLADFPPSTRTIDLERLLGNFENSGVVIRWVNDTVALAVFQTPSAALEALNHLRCPF 295
           HVI + DFP   RT DL R+  +++  G  I+WV+DT AL VF +P  A +AL       
Sbjct: 627 HVIEIYDFPQEFRTEDLLRIFCSYQKKGFDIKWVDDTHALGVFASPITARDALGTKHTMV 686

Query: 296 TVRILDENDTLLRS---IPPRDLEPPKQRPKTSVRAAQRLIAQGMGLKLPASMTAFGSRE 355
            +R L +     ++        L+P K+RP+TS   A+RL+   +G++   S T   +  
Sbjct: 687 KIRPLSQATRAAKAKARACAEFLQPAKERPETSAALARRLVISALGVRSKQSKTEREAEL 746

Query: 356 LRIQEEERRNRIESRRKLRDEAW 376
            ++QE   R R+E+  K R++ W
Sbjct: 747 RKLQEARERKRLEA--KQREDIW 767

BLAST of Tan0008221 vs. ExPASy Swiss-Prot
Match: Q7Z5L2 (Coiled-coil domain-containing protein R3HCC1L OS=Homo sapiens OX=9606 GN=R3HCC1L PE=1 SV=3)

HSP 1 Score: 73.6 bits (179), Expect = 5.6e-12
Identity = 55/191 (28.80%), Postives = 92/191 (48.17%), Query Frame = 0

Query: 189 GTFSYNKDELYSDKLSDSSTINDTNDEDASQVLEAR-RELKSAQYGTRHVIVLADFPPST 248
           G   + K E +  KLS ++   ++  E  S        ++  +     HVI + DFP   
Sbjct: 596 GILMHIKPENHCSKLSGNTKSRESIQEPRSDYYNHEVPDIDLSDCEFPHVIEIYDFPQEF 655

Query: 249 RTIDLERLLGNFENSGVVIRWVNDTVALAVFQTPSAALEALNHLRCPFTVRILDENDTLL 308
            T DL R+  +++  G  I+WV+DT AL VF +P  A +AL        +R L +     
Sbjct: 656 HTEDLLRVFCSYQKKGFDIKWVDDTHALGVFSSPITARDALGIKHTMVKIRPLSQATRAA 715

Query: 309 RS---IPPRDLEPPKQRPKTSVRAAQRLIAQGMGLKLPASMTAFGSRELRIQEEERRNRI 368
           ++        L+P K+RP+TS   A+RL+   +G++   S T   +   ++QE   R R+
Sbjct: 716 KAKARAYAEFLQPAKERPETSAALARRLVISALGVRSKQSKTEREAELKKLQEARERKRL 775

Query: 369 ESRRKLRDEAW 376
           E+  K R++ W
Sbjct: 776 EA--KQREDIW 784

BLAST of Tan0008221 vs. ExPASy Swiss-Prot
Match: Q8BSI6 (R3H and coiled-coil domain-containing protein 1 OS=Mus musculus OX=10090 GN=R3hcc1 PE=1 SV=3)

HSP 1 Score: 62.0 bits (149), Expect = 1.7e-08
Identity = 57/199 (28.64%), Postives = 96/199 (48.24%), Query Frame = 0

Query: 148 DDWEAIADRSPNELLSLQHEPDEPEISV-SETKDQTTRRRGRGTFSYNKDELYSDKLSDS 207
           D+ E   D    ++ S     DE E  V +E +D+       G FS    E+ ++     
Sbjct: 281 DEEEEKTDAQEGKVDSEGERMDEGEDKVDAEEEDEDEADADHGDFSELLQEITANLTEKE 340

Query: 208 STINDTN-DEDA-SQVLEARRELKSAQYGTRHVIVLADFPPSTRTIDLERLLGNFENSGV 267
             I   + D  A ++ L   R+L        H++ + DF P+ +T DL      F+  G 
Sbjct: 341 IKIEKIHLDTSAFTEELPGERDL-------THLVEIYDFKPTLKTEDLLATFSEFQEKGF 400

Query: 268 VIRWVNDTVALAVFQTPSAALEALNHLRCPFTVRILDE--NDTLLRSIP-PRDLEPPKQR 327
            I+WV+DT A+ +F  P++ALEAL        +R L +    + L+++  P+ L   K+R
Sbjct: 401 RIQWVDDTHAIGIFPCPASALEALAKDFSVLKIRPLTQGTKQSKLKALQRPKFLRLSKER 460

Query: 328 PKTSVRAAQRLIAQGMGLK 341
           P+T    A+RL+A+ +GL+
Sbjct: 461 PQTDSAVARRLVARALGLQ 472

BLAST of Tan0008221 vs. ExPASy Swiss-Prot
Match: Q9Y3T6 (R3H and coiled-coil domain-containing protein 1 OS=Homo sapiens OX=9606 GN=R3HCC1 PE=1 SV=3)

HSP 1 Score: 59.3 bits (142), Expect = 1.1e-07
Identity = 48/172 (27.91%), Postives = 87/172 (50.58%), Query Frame = 0

Query: 175 VSETKDQTTRRRGRGTFSYNKDELYSD---KLSDSSTINDTNDEDASQVLEARRELKSAQ 234
           V+E ++        G  S ++D+ YS+   +++D+ T  +   E       +  E    +
Sbjct: 255 VAEEEEDEEEVEEDGPSSCSEDD-YSELLQEITDNLTKKEIQIEKIHLDTSSFVEELPGE 314

Query: 235 YGTRHVIVLADFPPSTRTIDLERLLGNFENSGVVIRWVNDTVALAVFQTPSAALEALNHL 294
               HV+ + DF P+ +T DL      F+  G  I+WV+DT AL +F   ++A EAL   
Sbjct: 315 KDLAHVVEIYDFEPALKTEDLLATFSEFQEKGFRIQWVDDTHALGIFPCLASAAEALTRE 374

Query: 295 RCPFTVRILDE--NDTLLRSIP-PRDLEPPKQRPKTSVRAAQRLIAQGMGLK 341
                +R L +    + L+++  P+ L   K+RP+T+   A+RL+A+ +GL+
Sbjct: 375 FSVLKIRPLTQGTKQSKLKALQRPKLLRLVKERPQTNATVARRLVARALGLQ 425

BLAST of Tan0008221 vs. NCBI nr
Match: XP_038878405.1 (coiled-coil domain-containing protein R3HCC1L isoform X1 [Benincasa hispida])

HSP 1 Score: 568.9 bits (1465), Expect = 3.2e-158
Identity = 313/383 (81.72%), Postives = 336/383 (87.73%), Query Frame = 0

Query: 1   MDKEEEQQWPRSSNWSETVEDLVTAGDTDAAISLLEFVISDLQTSENRNPDPQLAPALTD 60
           MD EEE+Q  R SNWSETVEDLVTAGDTDAAISLLE V+SDLQTS+N NPDPQLA ALTD
Sbjct: 1   MDNEEERQ-RRRSNWSETVEDLVTAGDTDAAISLLESVVSDLQTSDNCNPDPQLAAALTD 60

Query: 61  LSALYSSKGFSLKADDIAAKALALKQQAQVSSSAGCSKIVKEDRTSPTNVSLSSRDSFNE 120
           LS LYSSKG SLKADDIAAKAL LKQQAQVS   G +K +KEDRTSPT +SLSS  S +E
Sbjct: 61  LSGLYSSKGLSLKADDIAAKALVLKQQAQVSCPTGHAK-MKEDRTSPTTISLSSCYSVDE 120

Query: 121 ASVRTGNLDHTRDSLDNALSCDGPLDDDDWEAIADRSPNELLSLQHEPDEPEISVSETKD 180
           ASV TGNLDHTRD+ DNA+ CDG LDDDDWEAIADR+PNELL LQ EPD+PE+SV ETKD
Sbjct: 121 ASVGTGNLDHTRDNPDNAVLCDGSLDDDDWEAIADRAPNELLPLQSEPDKPELSVKETKD 180

Query: 181 QTTRRRGRGTFSYNKDELYSDKLSDSSTINDTNDEDASQVLEA-RRELKSAQYGTRHVIV 240
           QT RRRGRGTFSYNK ELYSDKLSDSST NDTNDEDAS +LE   RELKSAQYGT+HV+V
Sbjct: 181 QTPRRRGRGTFSYNKHELYSDKLSDSSTTNDTNDEDASHMLEVEHRELKSAQYGTQHVLV 240

Query: 241 LADFPPSTRTIDLERLLGNFENSGVVIRWVNDTVALAVFQTPSAALEALNHLRCPFTVRI 300
           LADFP ST+T DLERLLGNF NSGVVIRWVNDTVALAVFQTPSAALE LNH+RCPFT+R 
Sbjct: 241 LADFPLSTKTTDLERLLGNFMNSGVVIRWVNDTVALAVFQTPSAALEVLNHVRCPFTLRQ 300

Query: 301 LDENDTLLRSIPPRDLEPPKQRPKTSVRAAQRLIAQGMGLKLPASMTAFGSRELRIQEEE 360
           L+END LL SIPPRDLEPPKQRPKTS R AQRLIAQGMGLKLP S T+FGS+ELR QEE+
Sbjct: 301 LEENDNLLSSIPPRDLEPPKQRPKTSARTAQRLIAQGMGLKLPLS-TSFGSKELRKQEED 360

Query: 361 RRNRIESRRKLRDEAWGDDEDPS 383
           RRNRI SR+KLRDEAWGDD DPS
Sbjct: 361 RRNRIVSRQKLRDEAWGDD-DPS 379

BLAST of Tan0008221 vs. NCBI nr
Match: XP_004139076.1 (coiled-coil domain-containing protein R3HCC1L isoform X1 [Cucumis sativus] >KAE8653547.1 hypothetical protein Csa_007454 [Cucumis sativus])

HSP 1 Score: 567.8 bits (1462), Expect = 7.2e-158
Identity = 308/382 (80.63%), Postives = 331/382 (86.65%), Query Frame = 0

Query: 1   MDKEEEQQWPRSSNWSETVEDLVTAGDTDAAISLLEFVISDLQTSENRNPDPQLAPALTD 60
           MDKE+E Q PR SNWSETVEDLVTAGDTDAAISLL+ V+SDLQTS+N NPDPQLA ALTD
Sbjct: 1   MDKEQEHQSPR-SNWSETVEDLVTAGDTDAAISLLQSVVSDLQTSQNSNPDPQLAAALTD 60

Query: 61  LSALYSSKGFSLKADDIAAKALALKQQAQVSSSAGCSKIVKEDRTSPTNVSLSSRDSFNE 120
           LSALYSSKG SLKADDIAAKA  LK QAQVS   G  KI+ EDRTSPT VSLSS D   E
Sbjct: 61  LSALYSSKGLSLKADDIAAKAFLLKHQAQVSCPTGYGKIMNEDRTSPTTVSLSSVD---E 120

Query: 121 ASVRTGNLDHTRDSLDNALSCDGPLDDDDWEAIADRSPNELLSLQHEPDEPEISVSETKD 180
           ASV TGNLD TRDS DNA+SC   LDDDDWEAIADR PNELLSL+ EPD+PE SV E K 
Sbjct: 121 ASVGTGNLDRTRDSPDNAVSCSASLDDDDWEAIADRPPNELLSLESEPDKPEQSVKEMKA 180

Query: 181 QTTRRRGRGTFSYNKDELYSDKLSDSSTINDTNDEDASQVLEARRELKSAQYGTRHVIVL 240
           QT RRRGRGTFSYNK ELYSDKLSDSST +DTN+E++S ++E RRELKSAQYGT+HV+VL
Sbjct: 181 QTPRRRGRGTFSYNKHELYSDKLSDSSTTDDTNEEESSHMIEGRRELKSAQYGTQHVLVL 240

Query: 241 ADFPPSTRTIDLERLLGNFENSGVVIRWVNDTVALAVFQTPSAALEALNHLRCPFTVRIL 300
           ADFPPST+TIDLERLLGNF NSGVVIRWVNDTVALAVFQTPS ALE LNH+RCPFT+R L
Sbjct: 241 ADFPPSTKTIDLERLLGNFMNSGVVIRWVNDTVALAVFQTPSTALEVLNHVRCPFTLRQL 300

Query: 301 DENDTLLRSIPPRDLEPPKQRPKTSVRAAQRLIAQGMGLKLPASMTAFGSRELRIQEEER 360
           DENDTLL SIPPRDL PPKQRPKTS R AQRLIAQGMGLKLP S T+FGS+ELR QEE+R
Sbjct: 301 DENDTLLSSIPPRDLVPPKQRPKTSARTAQRLIAQGMGLKLPNSTTSFGSKELRKQEEDR 360

Query: 361 RNRIESRRKLRDEAWGDDEDPS 383
           RNRI SR+KLRDEAWGDD DPS
Sbjct: 361 RNRIVSRQKLRDEAWGDD-DPS 377

BLAST of Tan0008221 vs. NCBI nr
Match: XP_022961195.1 (coiled-coil domain-containing protein R3HCC1L [Cucurbita moschata] >XP_022961196.1 coiled-coil domain-containing protein R3HCC1L [Cucurbita moschata] >XP_022961197.1 coiled-coil domain-containing protein R3HCC1L [Cucurbita moschata] >KAG6590134.1 Coiled-coil domain-containing protein R3HCC1L, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 564.7 bits (1454), Expect = 6.1e-157
Identity = 311/387 (80.36%), Postives = 332/387 (85.79%), Query Frame = 0

Query: 1   MDKEEEQQWPRSSNWSETVEDLVTAGDTDAAISLLEFVISDLQTSENRNPDPQLAPALTD 60
           MDKEEE+Q  R SNWSETVEDLVTAGDTDAAISLLE VISDLQTSEN NPDPQLA ALTD
Sbjct: 1   MDKEEERQ-QRRSNWSETVEDLVTAGDTDAAISLLESVISDLQTSENSNPDPQLAAALTD 60

Query: 61  LSALYSSKGFSLKADDIAAKALALKQQAQVSSSAGCSKIVKEDRTSPTNVSLSSRDSFNE 120
           LSALYSSKGFSLKADDIAAKAL LKQQ QVSSSAG +KIVKEDRTS   V+LSS +S +E
Sbjct: 61  LSALYSSKGFSLKADDIAAKALVLKQQDQVSSSAGFAKIVKEDRTSSATVTLSSHNSVDE 120

Query: 121 ASVRTGNLDHTRDSLDNALSCDGPLDDDDWEAIADRSPNELLSLQHEPDEPEISVSETKD 180
           ASV  G LDHTRD  DNA+ C    DDDDWEAIADR PNELLSLQ EPD+PEI + ET D
Sbjct: 121 ASVGIGTLDHTRDCPDNAVPC----DDDDWEAIADRPPNELLSLQCEPDKPEILIRETTD 180

Query: 181 Q---------TTRRRGRGTFSYNKDELYSDKLSDSSTINDTNDEDASQVLE-ARRELKSA 240
           Q         TTRRRGRGTFSY K ELYSD+LSDSST NDT DEDAS  LE  R+ELKSA
Sbjct: 181 QTTIRETTDKTTRRRGRGTFSYKKHELYSDRLSDSSTTNDTKDEDASHELEVGRKELKSA 240

Query: 241 QYGTRHVIVLADFPPSTRTIDLERLLGNFENSGVVIRWVNDTVALAVFQTPSAALEALNH 300
           QYGT+HV+VLADFPPST+TIDLERLLGNF+NSGVVIRWVNDTVALAVF TPS ALE LNH
Sbjct: 241 QYGTQHVLVLADFPPSTKTIDLERLLGNFKNSGVVIRWVNDTVALAVFPTPSTALEVLNH 300

Query: 301 LRCPFTVRILDENDTLLRSIPPRDLEPPKQRPKTSVRAAQRLIAQGMGLKLPASMTAFGS 360
           +RCPFT+R+LDENDTLL SIPPRDLEPPKQRPKTS R AQRLIAQGMGLKLPAS TAFGS
Sbjct: 301 VRCPFTIRLLDENDTLLSSIPPRDLEPPKQRPKTSARTAQRLIAQGMGLKLPASTTAFGS 360

Query: 361 RELRIQEEERRNRIESRRKLRDEAWGD 378
           +ELR QEE+RRNRI SR+KLRDEAWG+
Sbjct: 361 KELRKQEEDRRNRIVSRQKLRDEAWGE 382

BLAST of Tan0008221 vs. NCBI nr
Match: XP_023516771.1 (coiled-coil domain-containing protein R3HCC1L [Cucurbita pepo subsp. pepo] >XP_023516773.1 coiled-coil domain-containing protein R3HCC1L [Cucurbita pepo subsp. pepo] >XP_023516774.1 coiled-coil domain-containing protein R3HCC1L [Cucurbita pepo subsp. pepo])

HSP 1 Score: 564.3 bits (1453), Expect = 8.0e-157
Identity = 310/387 (80.10%), Postives = 331/387 (85.53%), Query Frame = 0

Query: 1   MDKEEEQQWPRSSNWSETVEDLVTAGDTDAAISLLEFVISDLQTSENRNPDPQLAPALTD 60
           MDKEEE+Q  R SNWSETVEDLVTAGDTDAAISLLE VISDLQTSEN NPDPQLA ALTD
Sbjct: 1   MDKEEERQ-QRRSNWSETVEDLVTAGDTDAAISLLESVISDLQTSENSNPDPQLAAALTD 60

Query: 61  LSALYSSKGFSLKADDIAAKALALKQQAQVSSSAGCSKIVKEDRTSPTNVSLSSRDSFNE 120
           LSALYSSKGFSLKADDIAAKAL LKQQ QVSS AG +KIVKEDRTS   VSLSS +S +E
Sbjct: 61  LSALYSSKGFSLKADDIAAKALVLKQQDQVSSPAGFAKIVKEDRTSSATVSLSSHNSVDE 120

Query: 121 ASVRTGNLDHTRDSLDNALSCDGPLDDDDWEAIADRSPNELLSLQHEPDEPEISVSETKD 180
           ASV TG LDHTRD  DNA+ C    DDDDWEAIADR PNELLSLQ EPD+PEI + ET D
Sbjct: 121 ASVGTGTLDHTRDCPDNAVPC----DDDDWEAIADRPPNELLSLQCEPDKPEILIRETTD 180

Query: 181 Q---------TTRRRGRGTFSYNKDELYSDKLSDSSTINDTNDEDASQVLE-ARRELKSA 240
           Q         TTRRRGRGTFSY K ELYSD+LSDSST NDT DEDAS  LE  R+ELKSA
Sbjct: 181 QTTIRETTDKTTRRRGRGTFSYKKHELYSDRLSDSSTTNDTKDEDASHELEVGRKELKSA 240

Query: 241 QYGTRHVIVLADFPPSTRTIDLERLLGNFENSGVVIRWVNDTVALAVFQTPSAALEALNH 300
           QYGT+HV+VLADFPPST+TIDLERLLGNF+NSGVVIRWVNDTVALAVF TPS ALE LNH
Sbjct: 241 QYGTQHVLVLADFPPSTKTIDLERLLGNFKNSGVVIRWVNDTVALAVFPTPSTALEVLNH 300

Query: 301 LRCPFTVRILDENDTLLRSIPPRDLEPPKQRPKTSVRAAQRLIAQGMGLKLPASMTAFGS 360
           +RCPFT+R+LDENDTLL S+PPRDLEPPKQRPKTS R AQRLIAQGMGLKLPAS T FGS
Sbjct: 301 VRCPFTIRLLDENDTLLSSVPPRDLEPPKQRPKTSARTAQRLIAQGMGLKLPASTTGFGS 360

Query: 361 RELRIQEEERRNRIESRRKLRDEAWGD 378
           +ELR QEE+RRNRI SR+KLRDEAWG+
Sbjct: 361 KELRKQEEDRRNRIVSRQKLRDEAWGE 382

BLAST of Tan0008221 vs. NCBI nr
Match: XP_008450290.1 (PREDICTED: coiled-coil domain-containing protein R3HCC1L isoform X1 [Cucumis melo])

HSP 1 Score: 564.3 bits (1453), Expect = 8.0e-157
Identity = 309/382 (80.89%), Postives = 329/382 (86.13%), Query Frame = 0

Query: 1   MDKEEEQQWPRSSNWSETVEDLVTAGDTDAAISLLEFVISDLQTSENRNPDPQLAPALTD 60
           MD E+E Q PR SNWSETVEDLVTAGDTDAAISLL+ V+SDLQ S+N NPDPQLA ALTD
Sbjct: 1   MDNEQEHQTPR-SNWSETVEDLVTAGDTDAAISLLQSVVSDLQISQNSNPDPQLAAALTD 60

Query: 61  LSALYSSKGFSLKADDIAAKALALKQQAQVSSSAGCSKIVKEDRTSPTNVSLSSRDSFNE 120
           LSALYSSKG SLKADDIAAKA  LK QAQVS   G  KIVKEDRTSPT VSLSS D   E
Sbjct: 61  LSALYSSKGLSLKADDIAAKAFLLKHQAQVSCPTGYGKIVKEDRTSPTTVSLSSVD---E 120

Query: 121 ASVRTGNLDHTRDSLDNALSCDGPLDDDDWEAIADRSPNELLSLQHEPDEPEISVSETKD 180
           ASV TGNLD T+DS DNA+SC   LDDDDWEAIADR PNELLSL+ EPD+ E SV E K 
Sbjct: 121 ASVGTGNLDRTKDSPDNAVSCAASLDDDDWEAIADRPPNELLSLESEPDKREPSVKEMKA 180

Query: 181 QTTRRRGRGTFSYNKDELYSDKLSDSSTINDTNDEDASQVLEARRELKSAQYGTRHVIVL 240
           QT RRRGRGTFSYNK ELYSDKLSDSST NDTN+EDAS++LE RRELKSAQYGT+HV+VL
Sbjct: 181 QTPRRRGRGTFSYNKHELYSDKLSDSSTTNDTNEEDASRMLEGRRELKSAQYGTQHVLVL 240

Query: 241 ADFPPSTRTIDLERLLGNFENSGVVIRWVNDTVALAVFQTPSAALEALNHLRCPFTVRIL 300
           ADFPPST+TIDLERLLGNF NSGVVIRWVNDTVALAVFQTPS ALE LNH+RCPFT+R L
Sbjct: 241 ADFPPSTKTIDLERLLGNFMNSGVVIRWVNDTVALAVFQTPSTALEVLNHVRCPFTLRQL 300

Query: 301 DENDTLLRSIPPRDLEPPKQRPKTSVRAAQRLIAQGMGLKLPASMTAFGSRELRIQEEER 360
           DENDTLL SIP RDL PPKQRPKTS R AQRLIAQGMGLKLP S T+FGS+ELR QEE+R
Sbjct: 301 DENDTLLSSIPQRDLVPPKQRPKTSARTAQRLIAQGMGLKLPHSTTSFGSKELRKQEEDR 360

Query: 361 RNRIESRRKLRDEAWGDDEDPS 383
           RNRI SR+KLRDEAWGDD DPS
Sbjct: 361 RNRIVSRQKLRDEAWGDD-DPS 377

BLAST of Tan0008221 vs. ExPASy TrEMBL
Match: A0A6J1H9N8 (coiled-coil domain-containing protein R3HCC1L OS=Cucurbita moschata OX=3662 GN=LOC111461782 PE=4 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 3.0e-157
Identity = 311/387 (80.36%), Postives = 332/387 (85.79%), Query Frame = 0

Query: 1   MDKEEEQQWPRSSNWSETVEDLVTAGDTDAAISLLEFVISDLQTSENRNPDPQLAPALTD 60
           MDKEEE+Q  R SNWSETVEDLVTAGDTDAAISLLE VISDLQTSEN NPDPQLA ALTD
Sbjct: 1   MDKEEERQ-QRRSNWSETVEDLVTAGDTDAAISLLESVISDLQTSENSNPDPQLAAALTD 60

Query: 61  LSALYSSKGFSLKADDIAAKALALKQQAQVSSSAGCSKIVKEDRTSPTNVSLSSRDSFNE 120
           LSALYSSKGFSLKADDIAAKAL LKQQ QVSSSAG +KIVKEDRTS   V+LSS +S +E
Sbjct: 61  LSALYSSKGFSLKADDIAAKALVLKQQDQVSSSAGFAKIVKEDRTSSATVTLSSHNSVDE 120

Query: 121 ASVRTGNLDHTRDSLDNALSCDGPLDDDDWEAIADRSPNELLSLQHEPDEPEISVSETKD 180
           ASV  G LDHTRD  DNA+ C    DDDDWEAIADR PNELLSLQ EPD+PEI + ET D
Sbjct: 121 ASVGIGTLDHTRDCPDNAVPC----DDDDWEAIADRPPNELLSLQCEPDKPEILIRETTD 180

Query: 181 Q---------TTRRRGRGTFSYNKDELYSDKLSDSSTINDTNDEDASQVLE-ARRELKSA 240
           Q         TTRRRGRGTFSY K ELYSD+LSDSST NDT DEDAS  LE  R+ELKSA
Sbjct: 181 QTTIRETTDKTTRRRGRGTFSYKKHELYSDRLSDSSTTNDTKDEDASHELEVGRKELKSA 240

Query: 241 QYGTRHVIVLADFPPSTRTIDLERLLGNFENSGVVIRWVNDTVALAVFQTPSAALEALNH 300
           QYGT+HV+VLADFPPST+TIDLERLLGNF+NSGVVIRWVNDTVALAVF TPS ALE LNH
Sbjct: 241 QYGTQHVLVLADFPPSTKTIDLERLLGNFKNSGVVIRWVNDTVALAVFPTPSTALEVLNH 300

Query: 301 LRCPFTVRILDENDTLLRSIPPRDLEPPKQRPKTSVRAAQRLIAQGMGLKLPASMTAFGS 360
           +RCPFT+R+LDENDTLL SIPPRDLEPPKQRPKTS R AQRLIAQGMGLKLPAS TAFGS
Sbjct: 301 VRCPFTIRLLDENDTLLSSIPPRDLEPPKQRPKTSARTAQRLIAQGMGLKLPASTTAFGS 360

Query: 361 RELRIQEEERRNRIESRRKLRDEAWGD 378
           +ELR QEE+RRNRI SR+KLRDEAWG+
Sbjct: 361 KELRKQEEDRRNRIVSRQKLRDEAWGE 382

BLAST of Tan0008221 vs. ExPASy TrEMBL
Match: A0A1S3BNW5 (coiled-coil domain-containing protein R3HCC1L isoform X1 OS=Cucumis melo OX=3656 GN=LOC103491941 PE=4 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 3.9e-157
Identity = 309/382 (80.89%), Postives = 329/382 (86.13%), Query Frame = 0

Query: 1   MDKEEEQQWPRSSNWSETVEDLVTAGDTDAAISLLEFVISDLQTSENRNPDPQLAPALTD 60
           MD E+E Q PR SNWSETVEDLVTAGDTDAAISLL+ V+SDLQ S+N NPDPQLA ALTD
Sbjct: 1   MDNEQEHQTPR-SNWSETVEDLVTAGDTDAAISLLQSVVSDLQISQNSNPDPQLAAALTD 60

Query: 61  LSALYSSKGFSLKADDIAAKALALKQQAQVSSSAGCSKIVKEDRTSPTNVSLSSRDSFNE 120
           LSALYSSKG SLKADDIAAKA  LK QAQVS   G  KIVKEDRTSPT VSLSS D   E
Sbjct: 61  LSALYSSKGLSLKADDIAAKAFLLKHQAQVSCPTGYGKIVKEDRTSPTTVSLSSVD---E 120

Query: 121 ASVRTGNLDHTRDSLDNALSCDGPLDDDDWEAIADRSPNELLSLQHEPDEPEISVSETKD 180
           ASV TGNLD T+DS DNA+SC   LDDDDWEAIADR PNELLSL+ EPD+ E SV E K 
Sbjct: 121 ASVGTGNLDRTKDSPDNAVSCAASLDDDDWEAIADRPPNELLSLESEPDKREPSVKEMKA 180

Query: 181 QTTRRRGRGTFSYNKDELYSDKLSDSSTINDTNDEDASQVLEARRELKSAQYGTRHVIVL 240
           QT RRRGRGTFSYNK ELYSDKLSDSST NDTN+EDAS++LE RRELKSAQYGT+HV+VL
Sbjct: 181 QTPRRRGRGTFSYNKHELYSDKLSDSSTTNDTNEEDASRMLEGRRELKSAQYGTQHVLVL 240

Query: 241 ADFPPSTRTIDLERLLGNFENSGVVIRWVNDTVALAVFQTPSAALEALNHLRCPFTVRIL 300
           ADFPPST+TIDLERLLGNF NSGVVIRWVNDTVALAVFQTPS ALE LNH+RCPFT+R L
Sbjct: 241 ADFPPSTKTIDLERLLGNFMNSGVVIRWVNDTVALAVFQTPSTALEVLNHVRCPFTLRQL 300

Query: 301 DENDTLLRSIPPRDLEPPKQRPKTSVRAAQRLIAQGMGLKLPASMTAFGSRELRIQEEER 360
           DENDTLL SIP RDL PPKQRPKTS R AQRLIAQGMGLKLP S T+FGS+ELR QEE+R
Sbjct: 301 DENDTLLSSIPQRDLVPPKQRPKTSARTAQRLIAQGMGLKLPHSTTSFGSKELRKQEEDR 360

Query: 361 RNRIESRRKLRDEAWGDDEDPS 383
           RNRI SR+KLRDEAWGDD DPS
Sbjct: 361 RNRIVSRQKLRDEAWGDD-DPS 377

BLAST of Tan0008221 vs. ExPASy TrEMBL
Match: A0A6J1JC58 (coiled-coil domain-containing protein R3HCC1L isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111485451 PE=4 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 9.5e-156
Identity = 307/389 (78.92%), Postives = 331/389 (85.09%), Query Frame = 0

Query: 1   MDKEEEQQWPRSSNWSETVEDLVTAGDTDAAISLLEFVISDLQTSENRNPDPQLAPALTD 60
           MDKEEE++  R SNWSETVEDLVTAGDTDAAISLLE V+SDLQTSEN NPDPQLA ALTD
Sbjct: 1   MDKEEERK-QRRSNWSETVEDLVTAGDTDAAISLLESVVSDLQTSENSNPDPQLAAALTD 60

Query: 61  LSALYSSKGFSLKADDIAAKALALKQQAQVSSSAGCSKIVKEDRTSPTNVSLSSRDSFNE 120
           LSALYSSKGFSLKADDI AKAL LKQ  QVSS AG +KIVKEDRTS   V+LSS +  +E
Sbjct: 61  LSALYSSKGFSLKADDIVAKALVLKQHDQVSSPAGFAKIVKEDRTSSATVTLSSHNLVDE 120

Query: 121 ASVRTGNLDHTRDSLDNALSCDGPLDDDDWEAIADRSPNELLSLQHEPDEPEISVSETKD 180
           ASV TG LDHTR+  DNA+ C    DDDDWEAIADR PNELLSLQ EPD+PEIS+ ET D
Sbjct: 121 ASVGTGTLDHTRNCPDNAVPC----DDDDWEAIADRPPNELLSLQCEPDKPEISIRETTD 180

Query: 181 Q---------TTRRRGRGTFSYNKDELYSDKLSDSSTINDTNDEDASQVLE-ARRELKSA 240
           Q         TTRRRGRGTFSY K ELYSD+LSDSST NDT DEDAS  LE  R+ELKSA
Sbjct: 181 QTTIRETTDKTTRRRGRGTFSYKKHELYSDRLSDSSTTNDTKDEDASHELEVGRKELKSA 240

Query: 241 QYGTRHVIVLADFPPSTRTIDLERLLGNFENSGVVIRWVNDTVALAVFQTPSAALEALNH 300
           QYGT+HV+VLADFPPST+TIDLERLLGNF+NSGVVIRWVNDTVALAVF TPS ALE LNH
Sbjct: 241 QYGTQHVLVLADFPPSTKTIDLERLLGNFKNSGVVIRWVNDTVALAVFPTPSTALEVLNH 300

Query: 301 LRCPFTVRILDENDTLLRSIPPRDLEPPKQRPKTSVRAAQRLIAQGMGLKLPASMTAFGS 360
           +RCPFT+R+LDENDTLL SIPPRDLEPPKQRPKTS R AQRLIAQGMGLKLPAS T FGS
Sbjct: 301 VRCPFTIRLLDENDTLLSSIPPRDLEPPKQRPKTSARTAQRLIAQGMGLKLPASTTGFGS 360

Query: 361 RELRIQEEERRNRIESRRKLRDEAWGDDE 380
           +ELR QEE+RRNRI SR+KLRDEAWGDD+
Sbjct: 361 KELRKQEEDRRNRIVSRQKLRDEAWGDDD 384

BLAST of Tan0008221 vs. ExPASy TrEMBL
Match: A0A6J1JIN7 (coiled-coil domain-containing protein R3HCC1L isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111485451 PE=4 SV=1)

HSP 1 Score: 552.4 bits (1422), Expect = 1.5e-153
Identity = 310/415 (74.70%), Postives = 333/415 (80.24%), Query Frame = 0

Query: 1   MDKEEEQQWPRSSNWSETVEDLVTAGDTDAAISLLEFVISDLQTSENRNPDPQLAPALTD 60
           MDKEEE++  R SNWSETVEDLVTAGDTDAAISLLE V+SDLQTSEN NPDPQLA ALTD
Sbjct: 1   MDKEEERK-QRRSNWSETVEDLVTAGDTDAAISLLESVVSDLQTSENSNPDPQLAAALTD 60

Query: 61  LSALYSSKGFSLKADDIAAKALALKQQAQVSSSAGCSKIVKEDRTSPTNVSLSSRDSFNE 120
           LSALYSSKGFSLKADDI AKAL LKQ  QVSS AG +KIVKEDRTS   V+LSS +  +E
Sbjct: 61  LSALYSSKGFSLKADDIVAKALVLKQHDQVSSPAGFAKIVKEDRTSSATVTLSSHNLVDE 120

Query: 121 ASVRTGNLDHTRDSLDNALSCDGPLDDDDWEAIADRSPNELLSLQHEPDEPEISVSETKD 180
           ASV TG LDHTR+  DNA+ C    DDDDWEAIADR PNELLSLQ EPD+PEIS+ ET D
Sbjct: 121 ASVGTGTLDHTRNCPDNAVPC----DDDDWEAIADRPPNELLSLQCEPDKPEISIRETTD 180

Query: 181 Q---------TTRRRGRGTFSYNKDELYSDKLSDSSTINDTNDEDASQVLE--------- 240
           Q         TTRRRGRGTFSY K ELYSD+LSDSST NDT DEDAS  LE         
Sbjct: 181 QTTIRETTDKTTRRRGRGTFSYKKHELYSDRLSDSSTTNDTKDEDASHELEVGRKELKSG 240

Query: 241 ---------------ARRELKSAQYGTRHVIVLADFPPSTRTIDLERLLGNFENSGVVIR 300
                           R+ELKSAQYGT+HV+VLADFPPST+TIDLERLLGNF+NSGVVIR
Sbjct: 241 KSSHKDEDASHELEVGRKELKSAQYGTQHVLVLADFPPSTKTIDLERLLGNFKNSGVVIR 300

Query: 301 WVNDTVALAVFQTPSAALEALNHLRCPFTVRILDENDTLLRSIPPRDLEPPKQRPKTSVR 360
           WVNDTVALAVF TPS ALE LNH+RCPFT+R+LDENDTLL SIPPRDLEPPKQRPKTS R
Sbjct: 301 WVNDTVALAVFPTPSTALEVLNHVRCPFTIRLLDENDTLLSSIPPRDLEPPKQRPKTSAR 360

Query: 361 AAQRLIAQGMGLKLPASMTAFGSRELRIQEEERRNRIESRRKLRDEAWGDDEDPS 383
            AQRLIAQGMGLKLPAS T FGS+ELR QEE+RRNRI SR+KLRDEAWGDD DPS
Sbjct: 361 TAQRLIAQGMGLKLPASTTGFGSKELRKQEEDRRNRIVSRQKLRDEAWGDD-DPS 409

BLAST of Tan0008221 vs. ExPASy TrEMBL
Match: A0A6J1JLB3 (coiled-coil domain-containing protein R3HCC1L isoform X4 OS=Cucurbita maxima OX=3661 GN=LOC111485451 PE=4 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 4.4e-153
Identity = 307/412 (74.51%), Postives = 331/412 (80.34%), Query Frame = 0

Query: 1   MDKEEEQQWPRSSNWSETVEDLVTAGDTDAAISLLEFVISDLQTSENRNPDPQLAPALTD 60
           MDKEEE++  R SNWSETVEDLVTAGDTDAAISLLE V+SDLQTSEN NPDPQLA ALTD
Sbjct: 1   MDKEEERK-QRRSNWSETVEDLVTAGDTDAAISLLESVVSDLQTSENSNPDPQLAAALTD 60

Query: 61  LSALYSSKGFSLKADDIAAKALALKQQAQVSSSAGCSKIVKEDRTSPTNVSLSSRDSFNE 120
           LSALYSSKGFSLKADDI AKAL LKQ  QVSS AG +KIVKEDRTS   V+LSS +  +E
Sbjct: 61  LSALYSSKGFSLKADDIVAKALVLKQHDQVSSPAGFAKIVKEDRTSSATVTLSSHNLVDE 120

Query: 121 ASVRTGNLDHTRDSLDNALSCDGPLDDDDWEAIADRSPNELLSLQHEPDEPEISVSETKD 180
           ASV TG LDHTR+  DNA+ C    DDDDWEAIADR PNELLSLQ EPD+PEIS+ ET D
Sbjct: 121 ASVGTGTLDHTRNCPDNAVPC----DDDDWEAIADRPPNELLSLQCEPDKPEISIRETTD 180

Query: 181 Q---------TTRRRGRGTFSYNKDELYSDKLSDSSTINDTNDEDASQVLE--------- 240
           Q         TTRRRGRGTFSY K ELYSD+LSDSST NDT DEDAS  LE         
Sbjct: 181 QTTIRETTDKTTRRRGRGTFSYKKHELYSDRLSDSSTTNDTKDEDASHELEVGRKELKSG 240

Query: 241 ---------------ARRELKSAQYGTRHVIVLADFPPSTRTIDLERLLGNFENSGVVIR 300
                           R+ELKSAQYGT+HV+VLADFPPST+TIDLERLLGNF+NSGVVIR
Sbjct: 241 KSSHKDEDASHELEVGRKELKSAQYGTQHVLVLADFPPSTKTIDLERLLGNFKNSGVVIR 300

Query: 301 WVNDTVALAVFQTPSAALEALNHLRCPFTVRILDENDTLLRSIPPRDLEPPKQRPKTSVR 360
           WVNDTVALAVF TPS ALE LNH+RCPFT+R+LDENDTLL SIPPRDLEPPKQRPKTS R
Sbjct: 301 WVNDTVALAVFPTPSTALEVLNHVRCPFTIRLLDENDTLLSSIPPRDLEPPKQRPKTSAR 360

Query: 361 AAQRLIAQGMGLKLPASMTAFGSRELRIQEEERRNRIESRRKLRDEAWGDDE 380
            AQRLIAQGMGLKLPAS T FGS+ELR QEE+RRNRI SR+KLRDEAWGDD+
Sbjct: 361 TAQRLIAQGMGLKLPASTTGFGSKELRKQEEDRRNRIVSRQKLRDEAWGDDD 407

BLAST of Tan0008221 vs. TAIR 10
Match: AT5G22120.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: cultured cell; Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 300.1 bits (767), Expect = 2.6e-81
Identity = 186/385 (48.31%), Postives = 251/385 (65.19%), Query Frame = 0

Query: 5   EEQQWPRSSNWSETVEDLVTAGDTDAAISLLEFVISDLQ-------TSENRNPDPQLAPA 64
           EE+      NWSE VEDLV AGD  AAIS LE + ++LQ       + E      QL+ A
Sbjct: 8   EEEGRISEPNWSERVEDLVVAGDVTAAISFLESLETNLQSRLGSSSSGERTEFGLQLSAA 67

Query: 65  LTDLSALYSSKGFSLKADDIAAKALALKQQA---QVSSSAGCSKIVKEDRTSPTNVSLSS 124
           LT L+ LYSS+G SLK+D++  ++  +KQ+A    ++SS     +  +   S    S  +
Sbjct: 68  LTQLADLYSSEGLSLKSDELRTRSSLIKQRALDCDLASSRSSGNVENQSVASSGLKSDPN 127

Query: 125 RDSFNEASVRTGNLDHTRDSLDNALSCDGPLDDDDWEAIADRSPNELLSLQHEPDEPEIS 184
             SF+      G  + ++ S  N+ + D    DDDWEA+AD  P++LL ++  P+  ++S
Sbjct: 128 VSSFD----ADGKTEDSKVSSSNSAAHDS--SDDDWEALADVEPSKLLPVEELPEISKLS 187

Query: 185 VSETKDQTTRRRGRGTFSYNKDELYSDK-LSDSSTINDTNDEDASQVLEARRELKSAQYG 244
           V E K Q  +RRGRGTF+Y  D +YSD+  S+S   +D+ D D S+  E   E   ++YG
Sbjct: 188 VEEPKVQGPKRRGRGTFTYKSDAMYSDRDFSESRFDDDSEDNDLSRESEKTDESLKSKYG 247

Query: 245 TRHVIVLADFPPSTRTIDLERLLGNFENSGVVIRWVNDTVALAVFQTPSAALEALNHLRC 304
           TRHV+VLA F PS RT +LE+L  +F++SG +IRWVNDT ALAVF+TPSAALEA  H++C
Sbjct: 248 TRHVLVLAGFSPSLRTTELEKLFKDFKDSGFIIRWVNDTTALAVFKTPSAALEACKHVQC 307

Query: 305 PFTVRILDENDTLLRSIPPRDLEPPKQRPKTSVRAAQRLIAQGMGLKLPASMTAFGSREL 364
            FT+R+LD+ND+LL SI  +DLEPP QRPKTS + AQRLIA  MGLKLPAS   FGS+E 
Sbjct: 308 SFTIRVLDDNDSLLGSISGKDLEPPSQRPKTSAKTAQRLIAHSMGLKLPAS--GFGSKE- 367

Query: 365 RIQEEERRNRIESRRKLRDEAWGDD 379
           R QE  R+NRI SR+K R++AWGDD
Sbjct: 368 RDQEAARKNRIVSRQKQREDAWGDD 383