Tan0008075 (gene) Snake gourd v1

BLAST of Tan0008075 vs. ExPASy Swiss-Prot
Match: Q9STW5 (MACPF domain-containing protein At4g24290 OS=Arabidopsis thaliana OX=3702 GN=At4g24290 PE=2 SV=1)

HSP 1 Score: 909.1 bits (2348), Expect = 2.7e-263
Identity = 448/598 (74.92%), Postives = 515/598 (86.12%), Query Frame = 0

Query: 1   MALRVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGLGH-ETVLPGGI 60
           MALR+  SKAAE+AIGSIG GYD+ IDLRLKYCKG    SRL++I EG  + E VLPGGI
Sbjct: 1   MALRLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGI 60

Query: 61  SISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQK 120
           SI NV KSIKCDKGER RFRSD+L FQQM+EQFNQE++L GKIPSG FN MFEFS CWQK
Sbjct: 61  SIPNVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQK 120

Query: 121 DAANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIENFGTHIIVG 180
           DAA TKNLAFDGVFI+LY+VAL+KSQ++L +HV++AVPS+WDPAALARFI+ +GTHIIV 
Sbjct: 121 DAAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVS 180

Query: 181 LKMGGKDVIYMKQQHSSTLQPSEVQKRLKDMADKRFSETIGQYGMGSERIYNNDKFEIRE 240
           +KMGGKDVIY KQQHSS LQP ++QKRLK++ADKRF E    +  GSER+  + K E +E
Sbjct: 181 VKMGGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKE 240

Query: 241 QHLRFADASSAIS-ALKEDIITIYKRRGGSDSINLPHHEWLQTVQLEPDVISVSFIPITS 300
           Q LRFAD SS  S A KED + + KRRGG+D+ NL H+EWLQTVQ+EPDVIS+SFIPITS
Sbjct: 241 QRLRFADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITS 300

Query: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360
           LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAP+FSELPLGPQRKQ + AS
Sbjct: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCAS 360

Query: 361 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPN 420
           LQFS  G KLYVNTTPVDVG+RP+TG+RLYLEG+RSN L+IHLQHLSSLPKI+QL +D N
Sbjct: 361 LQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLN 420

Query: 421 GNFRQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLRF 480
            + RQES+DRRY EKV  K +SHVCT PVE+D+DLS+VTGAQL V +HG +NVLFLRL F
Sbjct: 421 RSIRQESHDRRYYEKVNWKNYSHVCTEPVESDDDLSVVTGAQLHVESHGFKNVLFLRLCF 480

Query: 481 SSVLGATVVKQPEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPP 540
           S V+GAT+VK  EW+ + G A KSGLISTLISHHF+A QKPPP+PADVNINSA+YPGGPP
Sbjct: 481 SRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNINSAIYPGGPP 540

Query: 541 VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDE 597
           VP QAPKLLKFVDT+EM RGPQE+PGYWVVSGARL+VEKG+ISL+VK+SL T IL DE
Sbjct: 541 VPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSLFTPILGDE 598

BLAST of Tan0008075 vs. ExPASy Swiss-Prot
Match: Q9SGN6 (MACPF domain-containing protein NSL1 OS=Arabidopsis thaliana OX=3702 GN=NSL1 PE=2 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 5.1e-169
Identity = 318/606 (52.48%), Postives = 410/606 (67.66%), Query Frame = 0

Query: 4   RVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGLGHETVLPGGISISN 63
           R+    AAE A+  IG GYD+  D+R   CK    GSRL+EID     + + PGGI ++N
Sbjct: 8   RLDAHSAAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNN 67

Query: 64  VPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKDAAN 123
           V  SIKCDKGERTR RSD+LSF QMSE+FNQ+M L+GKIPSG FN MF FS CW KDA++
Sbjct: 68  VSSSIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASS 127

Query: 124 TKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIENFGTHIIVGLKMG 183
            K LA+DG FI+LY+V + + Q+ L D V++ VPSSWD AALA FIE +GTH++VG+ MG
Sbjct: 128 VKTLAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMG 187

Query: 184 GKDVIYMKQQHSSTLQPSEVQKRLKDMADKRFS----ETIGQYGMGSERIYNNDKFEIRE 243
           GKDVI++KQ   S  +P E+QK LK   D+RF     E+     + S +    +  +   
Sbjct: 188 GKDVIHVKQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQWGL 247

Query: 244 QHLRFADASSAISALK-EDIITIYKRRGGSDSINLPHHEWLQTVQLEPDVISVSFIPITS 303
           Q    + +S+ +   K E+I+ +  RRGG D +   H  WL TV   P+VIS+ F+PITS
Sbjct: 248 QPFGTSVSSAVVMHTKNEEIMRVCIRRGGVD-LGQSHERWLSTVSQAPNVISMCFVPITS 307

Query: 304 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 363
           LL+G+PG+GFLSHA+NLYLRYKPPIEELHQFLEFQLPRQWAP++ +LPLG +R + +  S
Sbjct: 308 LLSGLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPS 367

Query: 364 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPN 423
           LQFSLMG KLYVNT+ VD G RPVTGLR +LEGK+ N L+IHLQHLS+ P    L+ D  
Sbjct: 368 LQFSLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDT 427

Query: 424 GNFRQESYDRRYLEKVQSKYFSHVCTAPVE-----ADEDLSIVTGAQLQVVNHGMRNVLF 483
               +E  ++ Y   V+   FSHVCT PV+     +D+  SIVT A L+V   GMR VLF
Sbjct: 428 YEPIEEPVEKGYYVPVKWGIFSHVCTYPVQYNGARSDDTASIVTKAWLEVKGMGMRKVLF 487

Query: 484 LRLRFSSVLGATVVKQPEWEGSPGLAAKSGLISTLISHHFSAVQKPPP---KP-ADVNIN 543
           LRL F S+  + V ++  W+     + KSG+ S +IS   S    P P   KP + ++IN
Sbjct: 488 LRLGF-SLDASAVTRKSCWDNLSTNSRKSGVFS-MISTRLSTGLSPNPATTKPQSKIDIN 547

Query: 544 SAVYPGGPPVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLL 596
           SAVYP GP  PV+ PKLL  VDT E+ RGP+E PGYWVV+GA+L VE G+IS++ K+SLL
Sbjct: 548 SAVYPRGPSPPVK-PKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSLL 607

BLAST of Tan0008075 vs. ExPASy Swiss-Prot
Match: Q8L612 (MACPF domain-containing protein At1g14780 OS=Arabidopsis thaliana OX=3702 GN=At1g14780 PE=2 SV=1)

HSP 1 Score: 473.8 bits (1218), Expect = 2.9e-132
Identity = 269/619 (43.46%), Postives = 375/619 (60.58%), Query Frame = 0

Query: 12  EIAIGSIGRGYDITIDLRLKYCK---GNLHGSRLIEIDEGLGHETVLPGGISISNVPKSI 71
           E A+ S+G+G+D+T D RLKYCK   G+    RL+ +D+    E  +PG     NV   I
Sbjct: 10  ETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADI 69

Query: 72  KCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEF-SGCWQKDAANTKNL 131
            CDKGERTRFRSD+L F +MSE FNQ  ++TGKIPSG+FN  F F SG W  DAAN K+L
Sbjct: 70  NCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSL 129

Query: 132 AFDGVFITLYTVALEK-SQMVLCDHVRKAVPSSWDPAALARFIENFGTHIIVGLKMGGKD 191
             D   +TL+ + +   +++ L D VR AVPSSWDP  LARFIE +GTH+I G+ +GG+D
Sbjct: 130 GLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQD 189

Query: 192 VIYMKQQHSSTLQPSEVQKRLKDMADKRFSETIGQYGMGSERI-----YNNDKFEIREQH 251
           V+ ++Q  SS L    ++  L D+ D+ F+   G   + + R+     +++ + +  E  
Sbjct: 190 VVVVRQDKSSDLDNDLLRHHLYDLGDQLFT---GSCLLSTRRLNKAYHHSHSQPKFPEAF 249

Query: 252 LRFAD----ASSAISALKEDIITIYKRRGGSDSINLPHHEWLQTVQLEPDVISVSFIPIT 311
             F D    A +  S   ++ IT+   + G D     H EWL TV  +PD I+ +FIPIT
Sbjct: 250 NVFDDKQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPIT 309

Query: 312 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLA 371
           SLL  VPGSG LSHA++LYLRYKPP+ +L  FL+F  PR WAP+ ++LP G      N+A
Sbjct: 310 SLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFG---AAPNMA 369

Query: 372 S----LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIF-- 431
           S    L  + MG KLYVNTTPV   + PVTG+R +LEGK+ N L+IHLQHL +       
Sbjct: 370 SAYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGE 429

Query: 432 QLTEDPNGNFRQESYDR-RYLEKVQSKYFSHVCTAPVEADED--------------LSIV 491
           ++T++       +  D  RY E +  K FSHVCT PV+ D +                IV
Sbjct: 430 KITDEHIWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIV 489

Query: 492 TGAQLQVVNHGMRNVLFLRLRFSSVLGATVVKQPEWEGSPGLAAKSGLISTLI------S 551
           TGAQL+V  HG ++VL LRLR++ V    VV+     G  G + KSG+ S++       S
Sbjct: 490 TGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGS 549

Query: 552 HHFSAVQKPPPKPADVNINSAVYPGGPPVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSG 590
            H + +QK      +V ++S V+PGGPPVP    K++KFVD +++ RGPQ +PG+W+V+G
Sbjct: 550 VHHNMIQK---DKNEVVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHWLVTG 609

BLAST of Tan0008075 vs. ExPASy Swiss-Prot
Match: Q9C7N2 (MACPF domain-containing protein CAD1 OS=Arabidopsis thaliana OX=3702 GN=CAD1 PE=2 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 3.0e-129
Identity = 272/596 (45.64%), Postives = 355/596 (59.56%), Query Frame = 0

Query: 14  AIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGLGHETVLPGGISISNVPKSIKCDKG 73
           AI ++GRG+D+T D+RL YCKG   GSRL+ I+EG   +  L  G  + NVP  I C +G
Sbjct: 24  AIQALGRGFDVTSDVRLLYCKG-APGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRG 83

Query: 74  ERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKDAANTKNLAFDGVF 133
                R  V SF +M+E+FN    + G IP G FN MF ++G WQ DAA+TK+LA  G F
Sbjct: 84  NSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYF 143

Query: 134 ITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIENFGTHIIVGLKMGGKDVIYMKQQ 193
           I LY V L K  +VL + +R+AVPSSWDPA+LA FIEN+GTHI+  + +GG+DV+Y++Q 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 194 HSSTLQPSEVQKRLKDMADKRFSETIGQYGMGSERIYNNDKFEIREQHLRFADASSAISA 253
            SS L  SE++  + DM   RF E   Q   G                L++ D       
Sbjct: 204 QSSPLPVSEIENYVNDMIKHRFHEAESQSITGP---------------LKYKD------- 263

Query: 254 LKEDIITIYKRRGGSDSINLPHHEWLQTVQLEPDVISVSFIPITSLLNGVPGSGFLSHAI 313
             +DI  I++RRGG D +   H  W +TV   PD+I+++F PI SLL GVPG   L+ AI
Sbjct: 264 --KDITVIFRRRGG-DDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTRAI 323

Query: 314 NLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQFSLMGSKLYVNTT 373
            LYL YKPPIE+L  FL++Q+ R WAP  S L    QRK+   +SLQFSLMG KL+++  
Sbjct: 324 ELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLFISAD 383

Query: 374 PVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTED---PNGNFR---QESYD 433
            V VGR+PVTGLRL LEG + N LSIHLQHL SLPKI Q   D   P G  +    E  D
Sbjct: 384 QVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPEEQD 443

Query: 434 RRYLEKVQSKYFSHVCTAPVEADE----DLS---IVTGAQLQVVNHGMRNVLFLRLRFSS 493
            R+ E ++ K FSHV T+P+E  E    DLS   IVTGAQL V + G +NVL L+L FS 
Sbjct: 444 SRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLLFSK 503

Query: 494 VLGATVVKQPEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGP--- 553
           V G T +++  W+ +P   A SG +                            PGGP   
Sbjct: 504 VPGCT-IRRSVWDHTP--VASSGRLE---------------------------PGGPSTS 559

Query: 554 ----PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLL 590
                V  Q+ KL K VD++EM +GPQ+ PG+W+V+GA+L VEKG+I LRVK+SLL
Sbjct: 564 SSTEEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLL 559

BLAST of Tan0008075 vs. NCBI nr
Match: XP_004133715.1 (MACPF domain-containing protein At4g24290 [Cucumis sativus] >XP_011650569.1 MACPF domain-containing protein At4g24290 [Cucumis sativus] >KAE8650184.1 hypothetical protein Csa_010735 [Cucumis sativus])

HSP 1 Score: 1160.6 bits (3001), Expect = 0.0e+00
Identity = 576/605 (95.21%), Postives = 586/605 (96.86%), Query Frame = 0

Query: 1   MALRVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGLGHETVLPGGIS 60
           MAL+VHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEG+GHE VLPGGI 
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIL 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIENFGTHIIVGL 180
           AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIE FGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQ---PSEVQKRLKDMADKRFSETIGQYGMGSERIYNNDKFEI 240
           KMGGKDVIYMKQQHSSTLQ   PSEVQK LK+MADKRFSETI QYGMGSER+YNNDK EI
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240

Query: 241 REQHLRFADASSAISALKEDIITIYKRRGGSDSINLPHHEWLQTVQLEPDVISVSFIPIT 300
           REQHLRF DASSAIS LK DI+ I KRRGGSD I+LPHHEWLQTVQLEPDVIS+SFIPIT
Sbjct: 241 REQHLRFGDASSAISPLK-DIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPIT 300

Query: 301 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLA 360
           SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLA
Sbjct: 301 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLA 360

Query: 361 SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDP 420
           SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDP
Sbjct: 361 SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDP 420

Query: 421 NGNFRQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLR 480
           NGNFRQ+SYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVN GMRNVLFLRLR
Sbjct: 421 NGNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNQGMRNVLFLRLR 480

Query: 481 FSSVLGATVVKQPEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGP 540
           FSSVLGATVV+Q EWEGSPGLAAKSGLISTLISHHFS V KPPPKPADVNINSAVYPGGP
Sbjct: 481 FSSVLGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGP 540

Query: 541 PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEE 600
           PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEE
Sbjct: 541 PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEE 600

Query: 601 EINDH 603
           EIND+
Sbjct: 601 EINDN 604

BLAST of Tan0008075 vs. NCBI nr
Match: XP_023540812.1 (MACPF domain-containing protein At4g24290 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1159.8 bits (2999), Expect = 0.0e+00
Identity = 573/602 (95.18%), Postives = 584/602 (97.01%), Query Frame = 0

Query: 1   MALRVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGLGHETVLPGGIS 60
           MAL+VHPSKAAE+AIGSIGRGYDIT DLRLKYCKGN + SRLIEIDEG+GH  VLPGGIS
Sbjct: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFNDSRLIEIDEGVGHGIVLPGGIS 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIENFGTHIIVGL 180
           AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIE FGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEVQKRLKDMADKRFSETIGQYGMGSERIYNNDKFEIREQ 240
           KMGGKDVIYMKQQHSSTL+PSEVQKRLKDMADKRF+ET+GQYGMGSE IYNNDKFE REQ
Sbjct: 181 KMGGKDVIYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNNDKFESREQ 240

Query: 241 HLRFADASSAISALKEDIITIYKRRGGSDSINLPHHEWLQTVQLEPDVISVSFIPITSLL 300
           HL+F+DASSAISALKEDI+TIYKRRGGSD INLPHHEWLQTVQLEPDVISVSFIPITSLL
Sbjct: 241 HLQFSDASSAISALKEDIVTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLL 300

Query: 301 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQ 360
           NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQ NLASLQ
Sbjct: 301 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQ 360

Query: 361 FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGN 420
           FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPK FQLTEDPN N
Sbjct: 361 FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPNSN 420

Query: 421 FRQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLRFSS 480
             QESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRL F S
Sbjct: 421 SGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFFCS 480

Query: 481 VLGATVVKQPEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP 540
           VLGATVVKQ EWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP
Sbjct: 481 VLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP 540

Query: 541 VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEEIN 600
           VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVIL DEEEIN
Sbjct: 541 VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILSDEEEIN 600

Query: 601 DH 603
           D+
Sbjct: 601 DN 602

BLAST of Tan0008075 vs. NCBI nr
Match: KAG7031360.1 (MACPF domain-containing protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1157.9 bits (2994), Expect = 0.0e+00
Identity = 573/602 (95.18%), Postives = 583/602 (96.84%), Query Frame = 0

Query: 1   MALRVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGLGHETVLPGGIS 60
           MAL+VHPSKAAE+AIGSIGRGYDIT DLRLKYCKGN H SRLIEIDEG+GH  VLPGGIS
Sbjct: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHDSRLIEIDEGVGHGIVLPGGIS 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIENFGTHIIVGL 180
           AANTKNLAFDGVFITLYTVALEKSQMVL DHVRKAVPSSWDPAALARFIE FGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEVQKRLKDMADKRFSETIGQYGMGSERIYNNDKFEIREQ 240
           KMGGKDVIYMKQQHSSTL+PSEVQKRLKDMADKRF+ET+GQYGMGSE IYNNDKFE REQ
Sbjct: 181 KMGGKDVIYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNNDKFESREQ 240

Query: 241 HLRFADASSAISALKEDIITIYKRRGGSDSINLPHHEWLQTVQLEPDVISVSFIPITSLL 300
           HL+F+DASSAISALKEDI+TIYKRRGGSD INLPHHEWLQTVQLEPDVISVSFIPITSLL
Sbjct: 241 HLQFSDASSAISALKEDIVTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLL 300

Query: 301 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQ 360
           NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQ NLASLQ
Sbjct: 301 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQ 360

Query: 361 FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGN 420
           FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPK FQLTEDPN N
Sbjct: 361 FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPNSN 420

Query: 421 FRQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLRFSS 480
             QESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRL F S
Sbjct: 421 SGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFFCS 480

Query: 481 VLGATVVKQPEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP 540
           VLGATVVKQ EWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP
Sbjct: 481 VLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP 540

Query: 541 VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEEIN 600
           VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVIL DEEEIN
Sbjct: 541 VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILSDEEEIN 600

Query: 601 DH 603
           D+
Sbjct: 601 DN 602

BLAST of Tan0008075 vs. NCBI nr
Match: XP_022983823.1 (MACPF domain-containing protein At4g24290 [Cucurbita maxima])

HSP 1 Score: 1155.2 bits (2987), Expect = 0.0e+00
Identity = 570/602 (94.68%), Postives = 584/602 (97.01%), Query Frame = 0

Query: 1   MALRVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGLGHETVLPGGIS 60
           MAL+VHPSKAAE+AIGSIGRGYDIT DLRLKYCKGN HGSRLIEIDEG+GHE VLPGGIS
Sbjct: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGIS 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIENFGTHIIVGL 180
           AANTKNLAFDGVFITLYTVALEKSQMVL DHVRKAVPSSWDPAALARFIE FGTHIIVG 
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGF 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEVQKRLKDMADKRFSETIGQYGMGSERIYNNDKFEIREQ 240
           KMGGKDVIYMKQQHSSTL+PSEVQKRLKDMADKRF+ET+GQYGMGSE IYN++KFE REQ
Sbjct: 181 KMGGKDVIYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNDNKFESREQ 240

Query: 241 HLRFADASSAISALKEDIITIYKRRGGSDSINLPHHEWLQTVQLEPDVISVSFIPITSLL 300
           HL+F+DASSAISALKEDI+TIYKRRGGSD INLPHHEWLQTVQLEPDVISVSFIPITSLL
Sbjct: 241 HLQFSDASSAISALKEDILTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLL 300

Query: 301 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQ 360
           NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQ NLASLQ
Sbjct: 301 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQ 360

Query: 361 FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGN 420
           FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPK FQLTEDPN N
Sbjct: 361 FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPNNN 420

Query: 421 FRQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLRFSS 480
             QESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRL F S
Sbjct: 421 SGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFFCS 480

Query: 481 VLGATVVKQPEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP 540
           VLGATVVKQ EWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP
Sbjct: 481 VLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP 540

Query: 541 VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEEIN 600
           VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKG+ISLRVKFSLLTV+L DEEEIN
Sbjct: 541 VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGKISLRVKFSLLTVMLSDEEEIN 600

Query: 601 DH 603
           D+
Sbjct: 601 DN 602

BLAST of Tan0008075 vs. NCBI nr
Match: XP_008452249.1 (PREDICTED: MACPF domain-containing protein At4g24290 [Cucumis melo] >KAA0060601.1 MACPF domain-containing protein [Cucumis melo var. makuwa] >TYK02270.1 MACPF domain-containing protein [Cucumis melo var. makuwa])

HSP 1 Score: 1154.8 bits (2986), Expect = 0.0e+00
Identity = 572/605 (94.55%), Postives = 584/605 (96.53%), Query Frame = 0

Query: 1   MALRVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGLGHETVLPGGIS 60
           MAL+VHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEG+GHE VLPGGI 
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIV 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVP+SIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPESIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIENFGTHIIVGL 180
           AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIE FGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQ---PSEVQKRLKDMADKRFSETIGQYGMGSERIYNNDKFEI 240
           KMGGKDVIYMKQQHSSTLQ   PSEVQK LK+MADKRFSETI QYGMGSER+YNNDK EI
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240

Query: 241 REQHLRFADASSAISALKEDIITIYKRRGGSDSINLPHHEWLQTVQLEPDVISVSFIPIT 300
           REQHLRF DAS+AIS LK DI+ I KRRGGSD I+LPHHEWLQTVQLEPDVIS+SFIPIT
Sbjct: 241 REQHLRFGDASTAISPLK-DIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPIT 300

Query: 301 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLA 360
           SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLA
Sbjct: 301 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLA 360

Query: 361 SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDP 420
           SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDP
Sbjct: 361 SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDP 420

Query: 421 NGNFRQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLR 480
           NGNFRQ+SYDRRYLEKVQSKYFSHVCTAPVEADED SIVTGAQLQVVN GMRNVLFLRLR
Sbjct: 421 NGNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADEDFSIVTGAQLQVVNQGMRNVLFLRLR 480

Query: 481 FSSVLGATVVKQPEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGP 540
           FSSV GATVV+Q EWEGSPGLAAKSGLISTLISHHFS V KPPPKPADVNINSAVYPGGP
Sbjct: 481 FSSVFGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGP 540

Query: 541 PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEE 600
           PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEE
Sbjct: 541 PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEE 600

Query: 601 EINDH 603
           EIND+
Sbjct: 601 EINDN 604

BLAST of Tan0008075 vs. ExPASy TrEMBL
Match: A0A6J1J8T9 (MACPF domain-containing protein At4g24290 OS=Cucurbita maxima OX=3661 GN=LOC111482322 PE=4 SV=1)

HSP 1 Score: 1155.2 bits (2987), Expect = 0.0e+00
Identity = 570/602 (94.68%), Postives = 584/602 (97.01%), Query Frame = 0

Query: 1   MALRVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGLGHETVLPGGIS 60
           MAL+VHPSKAAE+AIGSIGRGYDIT DLRLKYCKGN HGSRLIEIDEG+GHE VLPGGIS
Sbjct: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGIS 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIENFGTHIIVGL 180
           AANTKNLAFDGVFITLYTVALEKSQMVL DHVRKAVPSSWDPAALARFIE FGTHIIVG 
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGF 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEVQKRLKDMADKRFSETIGQYGMGSERIYNNDKFEIREQ 240
           KMGGKDVIYMKQQHSSTL+PSEVQKRLKDMADKRF+ET+GQYGMGSE IYN++KFE REQ
Sbjct: 181 KMGGKDVIYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMGSEWIYNDNKFESREQ 240

Query: 241 HLRFADASSAISALKEDIITIYKRRGGSDSINLPHHEWLQTVQLEPDVISVSFIPITSLL 300
           HL+F+DASSAISALKEDI+TIYKRRGGSD INLPHHEWLQTVQLEPDVISVSFIPITSLL
Sbjct: 241 HLQFSDASSAISALKEDILTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLL 300

Query: 301 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQ 360
           NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQ NLASLQ
Sbjct: 301 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQ 360

Query: 361 FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGN 420
           FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPK FQLTEDPN N
Sbjct: 361 FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKSFQLTEDPNNN 420

Query: 421 FRQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLRFSS 480
             QESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRL F S
Sbjct: 421 SGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFFCS 480

Query: 481 VLGATVVKQPEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP 540
           VLGATVVKQ EWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP
Sbjct: 481 VLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP 540

Query: 541 VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEEIN 600
           VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKG+ISLRVKFSLLTV+L DEEEIN
Sbjct: 541 VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGKISLRVKFSLLTVMLSDEEEIN 600

Query: 601 DH 603
           D+
Sbjct: 601 DN 602

BLAST of Tan0008075 vs. ExPASy TrEMBL
Match: A0A5D3BT29 (MACPF domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold18G00720 PE=4 SV=1)

HSP 1 Score: 1154.8 bits (2986), Expect = 0.0e+00
Identity = 572/605 (94.55%), Postives = 584/605 (96.53%), Query Frame = 0

Query: 1   MALRVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGLGHETVLPGGIS 60
           MAL+VHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEG+GHE VLPGGI 
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIV 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVP+SIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPESIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIENFGTHIIVGL 180
           AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIE FGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQ---PSEVQKRLKDMADKRFSETIGQYGMGSERIYNNDKFEI 240
           KMGGKDVIYMKQQHSSTLQ   PSEVQK LK+MADKRFSETI QYGMGSER+YNNDK EI
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240

Query: 241 REQHLRFADASSAISALKEDIITIYKRRGGSDSINLPHHEWLQTVQLEPDVISVSFIPIT 300
           REQHLRF DAS+AIS LK DI+ I KRRGGSD I+LPHHEWLQTVQLEPDVIS+SFIPIT
Sbjct: 241 REQHLRFGDASTAISPLK-DIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPIT 300

Query: 301 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLA 360
           SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLA
Sbjct: 301 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLA 360

Query: 361 SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDP 420
           SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDP
Sbjct: 361 SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDP 420

Query: 421 NGNFRQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLR 480
           NGNFRQ+SYDRRYLEKVQSKYFSHVCTAPVEADED SIVTGAQLQVVN GMRNVLFLRLR
Sbjct: 421 NGNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADEDFSIVTGAQLQVVNQGMRNVLFLRLR 480

Query: 481 FSSVLGATVVKQPEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGP 540
           FSSV GATVV+Q EWEGSPGLAAKSGLISTLISHHFS V KPPPKPADVNINSAVYPGGP
Sbjct: 481 FSSVFGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGP 540

Query: 541 PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEE 600
           PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEE
Sbjct: 541 PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEE 600

Query: 601 EINDH 603
           EIND+
Sbjct: 601 EINDN 604

BLAST of Tan0008075 vs. ExPASy TrEMBL
Match: A0A1S3BTG2 (MACPF domain-containing protein At4g24290 OS=Cucumis melo OX=3656 GN=LOC103493321 PE=4 SV=1)

HSP 1 Score: 1154.8 bits (2986), Expect = 0.0e+00
Identity = 572/605 (94.55%), Postives = 584/605 (96.53%), Query Frame = 0

Query: 1   MALRVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGLGHETVLPGGIS 60
           MAL+VHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEG+GHE VLPGGI 
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGVGHEIVLPGGIV 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVP+SIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPESIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIENFGTHIIVGL 180
           AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIE FGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQ---PSEVQKRLKDMADKRFSETIGQYGMGSERIYNNDKFEI 240
           KMGGKDVIYMKQQHSSTLQ   PSEVQK LK+MADKRFSETI QYGMGSER+YNNDK EI
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPSEPSEVQKSLKEMADKRFSETIAQYGMGSERLYNNDKIEI 240

Query: 241 REQHLRFADASSAISALKEDIITIYKRRGGSDSINLPHHEWLQTVQLEPDVISVSFIPIT 300
           REQHLRF DAS+AIS LK DI+ I KRRGGSD I+LPHHEWLQTVQLEPDVIS+SFIPIT
Sbjct: 241 REQHLRFGDASTAISPLK-DIVPICKRRGGSDRISLPHHEWLQTVQLEPDVISMSFIPIT 300

Query: 301 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLA 360
           SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLA
Sbjct: 301 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLA 360

Query: 361 SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDP 420
           SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDP
Sbjct: 361 SLQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDP 420

Query: 421 NGNFRQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLR 480
           NGNFRQ+SYDRRYLEKVQSKYFSHVCTAPVEADED SIVTGAQLQVVN GMRNVLFLRLR
Sbjct: 421 NGNFRQDSYDRRYLEKVQSKYFSHVCTAPVEADEDFSIVTGAQLQVVNQGMRNVLFLRLR 480

Query: 481 FSSVLGATVVKQPEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGP 540
           FSSV GATVV+Q EWEGSPGLAAKSGLISTLISHHFS V KPPPKPADVNINSAVYPGGP
Sbjct: 481 FSSVFGATVVRQAEWEGSPGLAAKSGLISTLISHHFSTVMKPPPKPADVNINSAVYPGGP 540

Query: 541 PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEE 600
           PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEE
Sbjct: 541 PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEE 600

Query: 601 EINDH 603
           EIND+
Sbjct: 601 EINDN 604

BLAST of Tan0008075 vs. ExPASy TrEMBL
Match: A0A6J1FQI8 (MACPF domain-containing protein At4g24290 OS=Cucurbita moschata OX=3662 GN=LOC111447540 PE=4 SV=1)

HSP 1 Score: 1152.5 bits (2980), Expect = 0.0e+00
Identity = 571/602 (94.85%), Postives = 581/602 (96.51%), Query Frame = 0

Query: 1   MALRVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGLGHETVLPGGIS 60
           MAL+VHPSKAAE+AIGSIGRGYDIT DLRLKYCKGN H SRLIEIDEG+GH  VLPGGIS
Sbjct: 1   MALKVHPSKAAEVAIGSIGRGYDITTDLRLKYCKGNFHDSRLIEIDEGVGHGIVLPGGIS 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIENFGTHIIVGL 180
           AANTKNLAFDGVFITLYTVALEKSQMVL DHVRKAVPSSWDPAALARFIE FGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLRDHVRKAVPSSWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEVQKRLKDMADKRFSETIGQYGMGSERIYNNDKFEIREQ 240
           KMGGKDVIYMKQQHSSTL+PSEVQKRLKDMADKRF+ET+GQYGM SE IYNNDKFE REQ
Sbjct: 181 KMGGKDVIYMKQQHSSTLEPSEVQKRLKDMADKRFTETMGQYGMSSEWIYNNDKFESREQ 240

Query: 241 HLRFADASSAISALKEDIITIYKRRGGSDSINLPHHEWLQTVQLEPDVISVSFIPITSLL 300
           HL+F+DASSAISALKEDI+TIYKRRGGSD INLPHHEWLQTVQLEPDVISVSFIPITSLL
Sbjct: 241 HLQFSDASSAISALKEDIVTIYKRRGGSDRINLPHHEWLQTVQLEPDVISVSFIPITSLL 300

Query: 301 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQ 360
           NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQ NLASLQ
Sbjct: 301 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQRNLASLQ 360

Query: 361 FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGN 420
           FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLP  FQLTEDPN N
Sbjct: 361 FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPISFQLTEDPNSN 420

Query: 421 FRQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLRFSS 480
             QESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRL F S
Sbjct: 421 SGQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLFFCS 480

Query: 481 VLGATVVKQPEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP 540
           VLGATVVKQ EWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP
Sbjct: 481 VLGATVVKQAEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP 540

Query: 541 VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEEIN 600
           VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVIL DEEEIN
Sbjct: 541 VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILSDEEEIN 600

Query: 601 DH 603
           D+
Sbjct: 601 DN 602

BLAST of Tan0008075 vs. ExPASy TrEMBL
Match: A0A6J1JAX4 (MACPF domain-containing protein At4g24290-like OS=Cucurbita maxima OX=3661 GN=LOC111482823 PE=4 SV=1)

HSP 1 Score: 1122.1 bits (2901), Expect = 0.0e+00
Identity = 554/602 (92.03%), Postives = 567/602 (94.19%), Query Frame = 0

Query: 1   MALRVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGLGHETVLPGGIS 60
           MAL+VHPSKAAEIAIGSIGRGYDITIDLRLKYCKGN HGSRLIEIDEG+GHE VLPGGI 
Sbjct: 1   MALKVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNFHGSRLIEIDEGVGHEIVLPGGIL 60

Query: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKD 120
           ISNVPKSIKCDKGERTRFRSDVLSFQQMSE FNQEMTLTGKIPSGH NTMFEFSGCWQKD
Sbjct: 61  ISNVPKSIKCDKGERTRFRSDVLSFQQMSEHFNQEMTLTGKIPSGHLNTMFEFSGCWQKD 120

Query: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIENFGTHIIVGL 180
           AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRK VPS+WDPAALARFIE FGTHIIVGL
Sbjct: 121 AANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKEVPSTWDPAALARFIETFGTHIIVGL 180

Query: 181 KMGGKDVIYMKQQHSSTLQPSEVQKRLKDMADKRFSETIGQYGMGSERIYNNDKFEIREQ 240
           KMGGKDVIYMKQQHSSTLQP+EVQKRLK+MADKRFSETIGQYGMGSE+IYNNDK      
Sbjct: 181 KMGGKDVIYMKQQHSSTLQPTEVQKRLKEMADKRFSETIGQYGMGSEQIYNNDK------ 240

Query: 241 HLRFADASSAISALKEDIITIYKRRGGSDSINLPHHEWLQTVQLEPDVISVSFIPITSLL 300
                           DI+ IYKRRGGSD IN PHHEWLQTVQLEPDVIS+SFIPITSLL
Sbjct: 241 ----------------DILAIYKRRGGSDRINYPHHEWLQTVQLEPDVISMSFIPITSLL 300

Query: 301 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQ 360
           NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQ
Sbjct: 301 NGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQ 360

Query: 361 FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGN 420
           FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGN
Sbjct: 361 FSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPNGN 420

Query: 421 FRQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLRFSS 480
           F QESYDRRYLEKVQSKYFSHVCTAP+EADEDLSIVTGAQLQVVNHGM+NVLFLRLRFSS
Sbjct: 421 FCQESYDRRYLEKVQSKYFSHVCTAPIEADEDLSIVTGAQLQVVNHGMKNVLFLRLRFSS 480

Query: 481 VLGATVVKQPEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPPVP 540
           VLGATVV+QPEWEGSPGLAAKSGLISTLISHHFS VQKPPPKPADV INSAVYPGGPPVP
Sbjct: 481 VLGATVVRQPEWEGSPGLAAKSGLISTLISHHFSTVQKPPPKPADVIINSAVYPGGPPVP 540

Query: 541 VQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEEIN 600
           VQAPKLLKFVDTTEMKRGPQ+TPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEEIN
Sbjct: 541 VQAPKLLKFVDTTEMKRGPQDTPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDEEEIN 580

Query: 601 DH 603
           D+
Sbjct: 601 DN 580

BLAST of Tan0008075 vs. TAIR 10
Match: AT4G24290.2 (MAC/Perforin domain-containing protein )

HSP 1 Score: 909.1 bits (2348), Expect = 1.9e-264
Identity = 448/598 (74.92%), Postives = 515/598 (86.12%), Query Frame = 0

Query: 1   MALRVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGLGH-ETVLPGGI 60
           MALR+  SKAAE+AIGSIG GYD+ IDLRLKYCKG    SRL++I EG  + E VLPGGI
Sbjct: 1   MALRLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGI 60

Query: 61  SISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQK 120
           SI NV KSIKCDKGER RFRSD+L FQQM+EQFNQE++L GKIPSG FN MFEFS CWQK
Sbjct: 61  SIPNVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQK 120

Query: 121 DAANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIENFGTHIIVG 180
           DAA TKNLAFDGVFI+LY+VAL+KSQ++L +HV++AVPS+WDPAALARFI+ +GTHIIV 
Sbjct: 121 DAAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVS 180

Query: 181 LKMGGKDVIYMKQQHSSTLQPSEVQKRLKDMADKRFSETIGQYGMGSERIYNNDKFEIRE 240
           +KMGGKDVIY KQQHSS LQP ++QKRLK++ADKRF E    +  GSER+  + K E +E
Sbjct: 181 VKMGGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKE 240

Query: 241 QHLRFADASSAIS-ALKEDIITIYKRRGGSDSINLPHHEWLQTVQLEPDVISVSFIPITS 300
           Q LRFAD SS  S A KED + + KRRGG+D+ NL H+EWLQTVQ+EPDVIS+SFIPITS
Sbjct: 241 QRLRFADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITS 300

Query: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 360
           LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAP+FSELPLGPQRKQ + AS
Sbjct: 301 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPVFSELPLGPQRKQQSCAS 360

Query: 361 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPN 420
           LQFS  G KLYVNTTPVDVG+RP+TG+RLYLEG+RSN L+IHLQHLSSLPKI+QL +D N
Sbjct: 361 LQFSFFGPKLYVNTTPVDVGKRPITGMRLYLEGRRSNRLAIHLQHLSSLPKIYQLEDDLN 420

Query: 421 GNFRQESYDRRYLEKVQSKYFSHVCTAPVEADEDLSIVTGAQLQVVNHGMRNVLFLRLRF 480
            + RQES+DRRY EKV  K +SHVCT PVE+D+DLS+VTGAQL V +HG +NVLFLRL F
Sbjct: 421 RSIRQESHDRRYYEKVNWKNYSHVCTEPVESDDDLSVVTGAQLHVESHGFKNVLFLRLCF 480

Query: 481 SSVLGATVVKQPEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGPP 540
           S V+GAT+VK  EW+ + G A KSGLISTLISHHF+A QKPPP+PADVNINSA+YPGGPP
Sbjct: 481 SRVVGATLVKNSEWDEAVGFAPKSGLISTLISHHFTAAQKPPPRPADVNINSAIYPGGPP 540

Query: 541 VPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLLTVILPDE 597
           VP QAPKLLKFVDT+EM RGPQE+PGYWVVSGARL+VEKG+ISL+VK+SL T IL DE
Sbjct: 541 VPTQAPKLLKFVDTSEMTRGPQESPGYWVVSGARLLVEKGKISLKVKYSLFTPILGDE 598

BLAST of Tan0008075 vs. TAIR 10
Match: AT1G28380.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 595.9 bits (1535), Expect = 3.6e-170
Identity = 318/606 (52.48%), Postives = 410/606 (67.66%), Query Frame = 0

Query: 4   RVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGLGHETVLPGGISISN 63
           R+    AAE A+  IG GYD+  D+R   CK    GSRL+EID     + + PGGI ++N
Sbjct: 8   RLDAHSAAEKAVSVIGLGYDLCSDVRFSACKTTPDGSRLVEIDPTRNRDLIFPGGIVVNN 67

Query: 64  VPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKDAAN 123
           V  SIKCDKGERTR RSD+LSF QMSE+FNQ+M L+GKIPSG FN MF FS CW KDA++
Sbjct: 68  VSSSIKCDKGERTRLRSDILSFNQMSEKFNQDMCLSGKIPSGMFNNMFAFSKCWPKDASS 127

Query: 124 TKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIENFGTHIIVGLKMG 183
            K LA+DG FI+LY+V + + Q+ L D V++ VPSSWD AALA FIE +GTH++VG+ MG
Sbjct: 128 VKTLAYDGWFISLYSVEIVRKQLTLRDEVKREVPSSWDSAALAGFIEKYGTHVVVGVTMG 187

Query: 184 GKDVIYMKQQHSSTLQPSEVQKRLKDMADKRFS----ETIGQYGMGSERIYNNDKFEIRE 243
           GKDVI++KQ   S  +P E+QK LK   D+RF     E+     + S +    +  +   
Sbjct: 188 GKDVIHVKQMRKSNHEPEEIQKMLKHWGDERFCVDPVESKSPASVYSGKPKEENLLQWGL 247

Query: 244 QHLRFADASSAISALK-EDIITIYKRRGGSDSINLPHHEWLQTVQLEPDVISVSFIPITS 303
           Q    + +S+ +   K E+I+ +  RRGG D +   H  WL TV   P+VIS+ F+PITS
Sbjct: 248 QPFGTSVSSAVVMHTKNEEIMRVCIRRGGVD-LGQSHERWLSTVSQAPNVISMCFVPITS 307

Query: 304 LLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLAS 363
           LL+G+PG+GFLSHA+NLYLRYKPPIEELHQFLEFQLPRQWAP++ +LPLG +R + +  S
Sbjct: 308 LLSGLPGTGFLSHAVNLYLRYKPPIEELHQFLEFQLPRQWAPVYGDLPLGLRRSKQSSPS 367

Query: 364 LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTEDPN 423
           LQFSLMG KLYVNT+ VD G RPVTGLR +LEGK+ N L+IHLQHLS+ P    L+ D  
Sbjct: 368 LQFSLMGPKLYVNTSKVDSGERPVTGLRFFLEGKKGNHLAIHLQHLSACPPSLHLSHDDT 427

Query: 424 GNFRQESYDRRYLEKVQSKYFSHVCTAPVE-----ADEDLSIVTGAQLQVVNHGMRNVLF 483
               +E  ++ Y   V+   FSHVCT PV+     +D+  SIVT A L+V   GMR VLF
Sbjct: 428 YEPIEEPVEKGYYVPVKWGIFSHVCTYPVQYNGARSDDTASIVTKAWLEVKGMGMRKVLF 487

Query: 484 LRLRFSSVLGATVVKQPEWEGSPGLAAKSGLISTLISHHFSAVQKPPP---KP-ADVNIN 543
           LRL F S+  + V ++  W+     + KSG+ S +IS   S    P P   KP + ++IN
Sbjct: 488 LRLGF-SLDASAVTRKSCWDNLSTNSRKSGVFS-MISTRLSTGLSPNPATTKPQSKIDIN 547

Query: 544 SAVYPGGPPVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLL 596
           SAVYP GP  PV+ PKLL  VDT E+ RGP+E PGYWVV+GA+L VE G+IS++ K+SLL
Sbjct: 548 SAVYPRGPSPPVK-PKLLSLVDTKEVMRGPEEQPGYWVVTGAKLCVEAGKISIKAKYSLL 607

BLAST of Tan0008075 vs. TAIR 10
Match: AT1G14780.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 473.8 bits (1218), Expect = 2.1e-133
Identity = 269/619 (43.46%), Postives = 375/619 (60.58%), Query Frame = 0

Query: 12  EIAIGSIGRGYDITIDLRLKYCK---GNLHGSRLIEIDEGLGHETVLPGGISISNVPKSI 71
           E A+ S+G+G+D+T D RLKYCK   G+    RL+ +D+    E  +PG     NV   I
Sbjct: 10  ETAVKSLGKGFDLTADFRLKYCKDGDGSAGDDRLVVLDQTQNRELHIPGFGVFQNVSADI 69

Query: 72  KCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEF-SGCWQKDAANTKNL 131
            CDKGERTRFRSD+L F +MSE FNQ  ++TGKIPSG+FN  F F SG W  DAAN K+L
Sbjct: 70  NCDKGERTRFRSDILDFNKMSEYFNQRSSVTGKIPSGNFNATFGFQSGSWATDAANVKSL 129

Query: 132 AFDGVFITLYTVALEK-SQMVLCDHVRKAVPSSWDPAALARFIENFGTHIIVGLKMGGKD 191
             D   +TL+ + +   +++ L D VR AVPSSWDP  LARFIE +GTH+I G+ +GG+D
Sbjct: 130 GLDASVVTLFNLHIHNPNRLRLTDRVRNAVPSSWDPQLLARFIERYGTHVITGVSVGGQD 189

Query: 192 VIYMKQQHSSTLQPSEVQKRLKDMADKRFSETIGQYGMGSERI-----YNNDKFEIREQH 251
           V+ ++Q  SS L    ++  L D+ D+ F+   G   + + R+     +++ + +  E  
Sbjct: 190 VVVVRQDKSSDLDNDLLRHHLYDLGDQLFT---GSCLLSTRRLNKAYHHSHSQPKFPEAF 249

Query: 252 LRFAD----ASSAISALKEDIITIYKRRGGSDSINLPHHEWLQTVQLEPDVISVSFIPIT 311
             F D    A +  S   ++ IT+   + G D     H EWL TV  +PD I+ +FIPIT
Sbjct: 250 NVFDDKQTVAFNNFSINSQNGITVICAKRGGDGRAKSHSEWLITVPDKPDAINFNFIPIT 309

Query: 312 SLLNGVPGSGFLSHAINLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLA 371
           SLL  VPGSG LSHA++LYLRYKPP+ +L  FL+F  PR WAP+ ++LP G      N+A
Sbjct: 310 SLLKDVPGSGLLSHAMSLYLRYKPPLMDLQYFLDFSGPRAWAPVHNDLPFG---AAPNMA 369

Query: 372 S----LQFSLMGSKLYVNTTPVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIF-- 431
           S    L  + MG KLYVNTTPV   + PVTG+R +LEGK+ N L+IHLQHL +       
Sbjct: 370 SAYPALHINFMGPKLYVNTTPVTSEKNPVTGMRFFLEGKKCNRLAIHLQHLDNTRTTVGE 429

Query: 432 QLTEDPNGNFRQESYDR-RYLEKVQSKYFSHVCTAPVEADED--------------LSIV 491
           ++T++       +  D  RY E +  K FSHVCT PV+ D +                IV
Sbjct: 430 KITDEHIWRGSDQITDNDRYFEPLNGKKFSHVCTVPVKYDPNWIKTTSNHKSQNDVAFIV 489

Query: 492 TGAQLQVVNHGMRNVLFLRLRFSSVLGATVVKQPEWEGSPGLAAKSGLISTLI------S 551
           TGAQL+V  HG ++VL LRLR++ V    VV+     G  G + KSG+ S++       S
Sbjct: 490 TGAQLEVKKHGSKSVLHLRLRYTKVSDHYVVQNSWVHGPIGTSQKSGIFSSMSMPLTSGS 549

Query: 552 HHFSAVQKPPPKPADVNINSAVYPGGPPVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSG 590
            H + +QK      +V ++S V+PGGPPVP    K++KFVD +++ RGPQ +PG+W+V+G
Sbjct: 550 VHHNMIQK---DKNEVVLDSGVFPGGPPVPAN-NKIVKFVDLSQLCRGPQHSPGHWLVTG 609

BLAST of Tan0008075 vs. TAIR 10
Match: AT4G24290.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 466.5 bits (1199), Expect = 3.3e-131
Identity = 238/335 (71.04%), Postives = 277/335 (82.69%), Query Frame = 0

Query: 1   MALRVHPSKAAEIAIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGLGH-ETVLPGGI 60
           MALR+  SKAAE+AIGSIG GYD+ IDLRLKYCKG    SRL++I EG  + E VLPGGI
Sbjct: 1   MALRLPASKAAEVAIGSIGCGYDLAIDLRLKYCKGGSKDSRLLDIKEGDDNCEIVLPGGI 60

Query: 61  SISNVPKSIKCDKGERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQK 120
           SI NV KSIKCDKGER RFRSD+L FQQM+EQFNQE++L GKIPSG FN MFEFS CWQK
Sbjct: 61  SIPNVSKSIKCDKGERMRFRSDILPFQQMAEQFNQELSLAGKIPSGLFNAMFEFSSCWQK 120

Query: 121 DAANTKNLAFDGVFITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIENFGTHIIVG 180
           DAA TKNLAFDGVFI+LY+VAL+KSQ++L +HV++AVPS+WDPAALARFI+ +GTHIIV 
Sbjct: 121 DAAYTKNLAFDGVFISLYSVALDKSQVLLREHVKQAVPSTWDPAALARFIDIYGTHIIVS 180

Query: 181 LKMGGKDVIYMKQQHSSTLQPSEVQKRLKDMADKRFSETIGQYGMGSERIYNNDKFEIRE 240
           +KMGGKDVIY KQQHSS LQP ++QKRLK++ADKRF E    +  GSER+  + K E +E
Sbjct: 181 VKMGGKDVIYAKQQHSSKLQPEDLQKRLKEVADKRFVEASVVHNTGSERVQASSKVETKE 240

Query: 241 QHLRFADASSAIS-ALKEDIITIYKRRGGSDSINLPHHEWLQTVQLEPDVISVSFIPITS 300
           Q LRFAD SS  S A KED + + KRRGG+D+ NL H+EWLQTVQ+EPDVIS+SFIPITS
Sbjct: 241 QRLRFADTSSLGSYANKEDYVFMCKRRGGNDNRNLMHNEWLQTVQMEPDVISMSFIPITS 300

Query: 301 LLNGVPGSGFLSHAINLYLRYKP--PIEELHQFLE 332
           LLNGVPGSGFLSHAINLYLR KP   +  +H FLE
Sbjct: 301 LLNGVPGSGFLSHAINLYLRCKPINHLRFVHFFLE 335

BLAST of Tan0008075 vs. TAIR 10
Match: AT1G29690.1 (MAC/Perforin domain-containing protein )

HSP 1 Score: 463.8 bits (1192), Expect = 2.2e-130
Identity = 272/596 (45.64%), Postives = 355/596 (59.56%), Query Frame = 0

Query: 14  AIGSIGRGYDITIDLRLKYCKGNLHGSRLIEIDEGLGHETVLPGGISISNVPKSIKCDKG 73
           AI ++GRG+D+T D+RL YCKG   GSRL+ I+EG   +  L  G  + NVP  I C +G
Sbjct: 24  AIQALGRGFDVTSDVRLLYCKG-APGSRLVRIEEGQNRDLELSHGFLLPNVPADIDCSRG 83

Query: 74  ERTRFRSDVLSFQQMSEQFNQEMTLTGKIPSGHFNTMFEFSGCWQKDAANTKNLAFDGVF 133
                R  V SF +M+E+FN    + G IP G FN MF ++G WQ DAA+TK+LA  G F
Sbjct: 84  NSGTQRISVCSFHEMAEEFNVRSGVKGNIPLGCFNAMFNYTGSWQVDAASTKSLALVGYF 143

Query: 134 ITLYTVALEKSQMVLCDHVRKAVPSSWDPAALARFIENFGTHIIVGLKMGGKDVIYMKQQ 193
           I LY V L K  +VL + +R+AVPSSWDPA+LA FIEN+GTHI+  + +GG+DV+Y++Q 
Sbjct: 144 IPLYDVKLAKLTLVLHNEIRRAVPSSWDPASLASFIENYGTHIVTSVTIGGRDVVYIRQH 203

Query: 194 HSSTLQPSEVQKRLKDMADKRFSETIGQYGMGSERIYNNDKFEIREQHLRFADASSAISA 253
            SS L  SE++  + DM   RF E   Q   G                L++ D       
Sbjct: 204 QSSPLPVSEIENYVNDMIKHRFHEAESQSITGP---------------LKYKD------- 263

Query: 254 LKEDIITIYKRRGGSDSINLPHHEWLQTVQLEPDVISVSFIPITSLLNGVPGSGFLSHAI 313
             +DI  I++RRGG D +   H  W +TV   PD+I+++F PI SLL GVPG   L+ AI
Sbjct: 264 --KDITVIFRRRGG-DDLEQSHARWAETVPAAPDIINMTFTPIVSLLEGVPGLRHLTRAI 323

Query: 314 NLYLRYKPPIEELHQFLEFQLPRQWAPIFSELPLGPQRKQHNLASLQFSLMGSKLYVNTT 373
            LYL YKPPIE+L  FL++Q+ R WAP  S L    QRK+   +SLQFSLMG KL+++  
Sbjct: 324 ELYLEYKPPIEDLQYFLDYQIARAWAPEQSNL----QRKEPVCSSLQFSLMGPKLFISAD 383

Query: 374 PVDVGRRPVTGLRLYLEGKRSNCLSIHLQHLSSLPKIFQLTED---PNGNFR---QESYD 433
            V VGR+PVTGLRL LEG + N LSIHLQHL SLPKI Q   D   P G  +    E  D
Sbjct: 384 QVTVGRKPVTGLRLSLEGSKQNRLSIHLQHLVSLPKILQPHWDSHVPIGAPKWQGPEEQD 443

Query: 434 RRYLEKVQSKYFSHVCTAPVEADE----DLS---IVTGAQLQVVNHGMRNVLFLRLRFSS 493
            R+ E ++ K FSHV T+P+E  E    DLS   IVTGAQL V + G +NVL L+L FS 
Sbjct: 444 SRWFEPIKWKNFSHVSTSPIEHTETHIGDLSGVHIVTGAQLGVWDFGSKNVLHLKLLFSK 503

Query: 494 VLGATVVKQPEWEGSPGLAAKSGLISTLISHHFSAVQKPPPKPADVNINSAVYPGGP--- 553
           V G T +++  W+ +P   A SG +                            PGGP   
Sbjct: 504 VPGCT-IRRSVWDHTP--VASSGRLE---------------------------PGGPSTS 559

Query: 554 ----PVPVQAPKLLKFVDTTEMKRGPQETPGYWVVSGARLMVEKGRISLRVKFSLL 590
                V  Q+ KL K VD++EM +GPQ+ PG+W+V+GA+L VEKG+I LRVK+SLL
Sbjct: 564 SSTEEVSGQSGKLAKIVDSSEMLKGPQDLPGHWLVTGAKLGVEKGKIVLRVKYSLL 559