Tan0007977 (gene) Snake gourd v1

Overview
NameTan0007977
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptiontRNA_int_end_N2 domain-containing protein
LocationLG10: 66826355 .. 66828436 (+)
RNA-Seq ExpressionTan0007977
SyntenyTan0007977
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGCCTACGGATAGGGAAAGCTCTTCAGGAGCTAGTGGCGATGACGACATTTACAAGCAAGACATAAGAGATGAAGAAGAATGTCTTTGTTCATTTGGGTACATGCGCAAGTTGCAATTCAGGTTGAGAATTTCAAGAATTTAGACTCCCGGGAATCCTTCAATAGGATGGAGCTATTTGCTTATTTTTAAGTGTGTGTTTCAATATTTTTATCTGTCTCTAGGAAGCATGCTTCGACTGCTCGATGGAATGATCAGATGGGAATGGCAGAAGTTGTAGAGAACAAGGGCAACCTTTGGACGACGACTGGCATTGTACGTTGGGGCAAGATTTATTGTTCCATTGAGGAAACTTTGTAGGTCAAACTATGATTTTATTATGGTTTTGACATCTCTATTGGTTTTGATATTGATTACAACATTTTTTGGGGAGTTATTCTCTTTCACATTGTCTTTAAGTGATTACTATAAGATAAGGTAATATCATGAAAAATAAAAGAAATTGGTAGTGTTTTGGAGAGTTTTTTTTGTTGCTTTTTGGTTCTTTATTTAGAACATTACAATACAGATAAAGGGAAGAAATTCAAGCTTACAATGTACACGACGAAGCACTAGTAGTAGTCTTTATGTTTTGGTATATAAATGAATTCATCATTTTTTAGTACTTTTTAACTAAAGTCTTTTTTCCTATCATTATCTCTGTAGCAATGAAATTTTATTGAGCTTATTTGTGTCCCACTGTATAATTCATTTTATGTATCACTTATTCTTCTTTTCTATTTACAATATATACACATCTATTGTCACTTGGGTAATGTTTAATTTAATGTGTCTCTTGTTTCAGAAAAGTTAGAAGTGTGTTGCCTTGAAGTGTGCCTTGTGCTTTCTAAATGTTGACTGTAGTATGAACCTTGGCTCAAAAATAGAGTTGTACCATTTAAGCAGAAATATGTAGGATCATGATCTCTTTTGAGACATTTAAATGACAATTTTTAAGCATGCTATCAAACATTCTTTTCTATGTTTTCAGATTTCTTATTGAAGTTGGGGCCTTGCATCTTCTGGATTATGATAATTTAAATCTTTCTTTGAAAGATGTATATAAGAAGGTAGCTGAAGGAAAAAATGGATGTCTTTGGGAGCAGTTTGAGGTTTATAGGCACCTCAAATCTCTTGGTTTCATTGTTGGAAAGCATAAAGTTCCTTGGTCTGTGAAGGGTGTTAGGAATGGAAATGACATTTCTTCTCGAAGTTCTATATTTGAGAACAAAGGAGCGACAAATTTTGAATCAGAAGATGAGAGGTCGATCTCTGAGCTATTAGATTCCACTCAACTAAATGAAGTGACACCCATTTTTTATGTTTTTCTTCCACATAGAAAATTTAGAAAATCTTCTCCTGGTGACCCAAATTTTATGGTCTGCTTGACTAAGTAAATTCTTGTGCCAAAAATCATTGTAATTCCTTAAGATTCTGTCCGGTTGGTTATTTTTTTTGCATGTTGTCTTTTTATTTTTGTGTAAACTTCTTGGAAGGCATAGTTAAAAATGACTGGCACCTGATTTTCCTTTTTTAATAACTATTGTCCTCTGATTTGACTTTTTAATGAAATTTCATTTCAAAATAATATTAATAATAATAATAATAGTAATAATAATAATAATTTGCAAAAGGTGTTTTTTAGTGAGGCATGTAGGAATAGGATGTAAGCAGTTTTGTTGTTAAATTGTATACACAATAGATCAAATTCATTTTTAGCCTTCTGAGTATTTCGCATCATTTTTTAGGAAAATAGTAGGTTGCATTCTTGAAATAACACCAAAATGAGGTCTATAATAGGCTTCAACTTGTGTGGTTTCTTTCTTCTGCAAAATTTTTATAGATTACTCAACTTAGAATTTGTCTAATCCTTTAATTACTGTGATATTATTTTTTTTATAGGGGATACCCACCTCAAAGAAAGAATATTGAAATTATTGATAGAACAACGAGAGGCATTCCAATGAAATATTGTCATGTTGAACATGGACGTGTTTGTTTCTTCTCACTTGATAAGGTGGAGTTGCCCATCTTACCGTGA

mRNA sequence

ATGGAGCCTACGGATAGGGAAAGCTCTTCAGGAGCTAGTGGCGATGACGACATTTACAAGCAAGACATAAGAGATGAAGAAGAATGTCTTTGTTCATTTGGGTACATGCGCAAGTTGCAATTCAGGAAGCATGCTTCGACTGCTCGATGGAATGATCAGATGGGAATGGCAGAAGTTGTAGAGAACAAGGGCAACCTTTGGACGACGACTGGCATTGTACGTTGGGGCAAGATTTATTGTTCCATTGAGGAAACTTTATTTCTTATTGAAGTTGGGGCCTTGCATCTTCTGGATTATGATAATTTAAATCTTTCTTTGAAAGATGTATATAAGAAGGTAGCTGAAGGAAAAAATGGATGTCTTTGGGAGCAGTTTGAGGTTTATAGGCACCTCAAATCTCTTGGTTTCATTGTTGGAAAGCATAAAGTTCCTTGGTCTGTGAAGGGTGTTAGGAATGGAAATGACATTTCTTCTCGAAGTTCTATATTTGAGAACAAAGGAGCGACAAATTTTGAATCAGAAGATGAGAGGTCGATCTCTGAGCTATTAGATTCCACTCAACTAAATGAAGTGACACCCATTTTTTATGTTTTTCTTCCACATAGAAAATTTAGAAAATCTTCTCCTGGTGACCCAAATTTTATGGTCTGCTTGACTAAGGGATACCCACCTCAAAGAAAGAATATTGAAATTATTGATAGAACAACGAGAGGCATTCCAATGAAATATTGTCATGTTGAACATGGACGTGTTTGTTTCTTCTCACTTGATAAGGTGGAGTTGCCCATCTTACCGTGA

Coding sequence (CDS)

ATGGAGCCTACGGATAGGGAAAGCTCTTCAGGAGCTAGTGGCGATGACGACATTTACAAGCAAGACATAAGAGATGAAGAAGAATGTCTTTGTTCATTTGGGTACATGCGCAAGTTGCAATTCAGGAAGCATGCTTCGACTGCTCGATGGAATGATCAGATGGGAATGGCAGAAGTTGTAGAGAACAAGGGCAACCTTTGGACGACGACTGGCATTGTACGTTGGGGCAAGATTTATTGTTCCATTGAGGAAACTTTATTTCTTATTGAAGTTGGGGCCTTGCATCTTCTGGATTATGATAATTTAAATCTTTCTTTGAAAGATGTATATAAGAAGGTAGCTGAAGGAAAAAATGGATGTCTTTGGGAGCAGTTTGAGGTTTATAGGCACCTCAAATCTCTTGGTTTCATTGTTGGAAAGCATAAAGTTCCTTGGTCTGTGAAGGGTGTTAGGAATGGAAATGACATTTCTTCTCGAAGTTCTATATTTGAGAACAAAGGAGCGACAAATTTTGAATCAGAAGATGAGAGGTCGATCTCTGAGCTATTAGATTCCACTCAACTAAATGAAGTGACACCCATTTTTTATGTTTTTCTTCCACATAGAAAATTTAGAAAATCTTCTCCTGGTGACCCAAATTTTATGGTCTGCTTGACTAAGGGATACCCACCTCAAAGAAAGAATATTGAAATTATTGATAGAACAACGAGAGGCATTCCAATGAAATATTGTCATGTTGAACATGGACGTGTTTGTTTCTTCTCACTTGATAAGGTGGAGTTGCCCATCTTACCGTGA

Protein sequence

MEPTDRESSSGASGDDDIYKQDIRDEEECLCSFGYMRKLQFRKHASTARWNDQMGMAEVVENKGNLWTTTGIVRWGKIYCSIEETLFLIEVGALHLLDYDNLNLSLKDVYKKVAEGKNGCLWEQFEVYRHLKSLGFIVGKHKVPWSVKGVRNGNDISSRSSIFENKGATNFESEDERSISELLDSTQLNEVTPIFYVFLPHRKFRKSSPGDPNFMVCLTKGYPPQRKNIEIIDRTTRGIPMKYCHVEHGRVCFFSLDKVELPILP
Homology
BLAST of Tan0007977 vs. ExPASy Swiss-Prot
Match: O74908 (Probable tRNA-splicing endonuclease subunit sen54 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=sen54 PE=3 SV=1)

HSP 1 Score: 53.5 bits (127), Expect = 4.2e-06
Identity = 37/104 (35.58%), Postives = 55/104 (52.88%), Query Frame = 0

Query: 36  MRKLQFRKHASTARWNDQMGMAEVVENKGNLWTTTGIV-RWGKIYCSIEETLFLIEVGAL 95
           + +L   KHA  A WN Q GM+ V +  G L+ T G      +++   EETL+L+E G++
Sbjct: 74  VERLISAKHAIIATWNAQNGMSCVEKAHGPLFKTMGTADSQNRMWLLPEETLYLVERGSM 133

Query: 96  HLLDYDNLNLSLKDVYKKVAEGKNGCLWEQFEVYRHLKSLGFIV 139
                + L +SL+ VY   +    G L E + VY HL+  GF V
Sbjct: 134 ECWSEEGLPMSLQAVY-SASIPLCGSL-ENYLVYAHLRRCGFSV 175

BLAST of Tan0007977 vs. ExPASy Swiss-Prot
Match: Q74ZJ5 (tRNA-splicing endonuclease subunit SEN54 OS=Ashbya gossypii (strain ATCC 10895 / CBS 109.51 / FGSC 9923 / NRRL Y-1056) OX=284811 GN=SEN54 PE=3 SV=1)

HSP 1 Score: 46.6 bits (109), Expect = 5.1e-04
Identity = 29/102 (28.43%), Postives = 53/102 (51.96%), Query Frame = 0

Query: 48  ARWNDQMGMAEVVENKGNLWTTTGIV-RWGKIYCSIEETLFLIEVGAL----------HL 107
           A W ++  MA +   +G+   T G V + G+ +  + E ++L+E G +            
Sbjct: 88  AYWEEEQRMARIPNARGSFTNTMGKVDKHGQCWLQLHEFVYLVERGTVSPYLALTVGDEK 147

Query: 108 LDYDNLNLSLKDVYKKVAEGKNGCLWEQFEVYRHLKSLGFIV 139
            +++++ LS++DVY   +  +     ++F VY HLK LGFIV
Sbjct: 148 SNHEDVLLSVQDVYALFSSTEE---LDEFLVYAHLKRLGFIV 186

BLAST of Tan0007977 vs. NCBI nr
Match: XP_022156818.1 (uncharacterized protein LOC111023660 [Momordica charantia])

HSP 1 Score: 453.4 bits (1165), Expect = 1.4e-123
Identity = 218/266 (81.95%), Postives = 244/266 (91.73%), Query Frame = 0

Query: 1   MEPTDRESSS-GASGDDDIYKQDIRDEEECLCSFGYMRKLQFRKHASTARWNDQMGMAEV 60
           ME TD ESSS GASGDD+IY+QD+ DEEECLC+ G MRKLQFRKHASTARWNDQMGMAEV
Sbjct: 1   MEATDWESSSGGASGDDEIYEQDVEDEEECLCASGNMRKLQFRKHASTARWNDQMGMAEV 60

Query: 61  VENKGNLWTTTGIVRWGKIYCSIEETLFLIEVGALHLLDYDNLNLSLKDVYKKVAEGKNG 120
           +EN+G+LWTTTGIVR GKIYCS EETLFL+EVGALHLLD+DN +LSLKDVYKKVAEGKNG
Sbjct: 61  LENRGSLWTTTGIVRCGKIYCSFEETLFLMEVGALHLLDHDNSSLSLKDVYKKVAEGKNG 120

Query: 121 CLWEQFEVYRHLKSLGFIVGKHKVPWSVKGVRNGNDISSRSSIFENKGATNFESEDERSI 180
           CLWEQFEVYRHLKSLGFIVGKHKVPWSVKGVRNG+DIS +SSI EN+GA + ES+DERSI
Sbjct: 121 CLWEQFEVYRHLKSLGFIVGKHKVPWSVKGVRNGSDISPQSSI-ENEGAWDLESKDERSI 180

Query: 181 SELLDSTQLNEVTPIFYVFLPHRKFRKSSPGDPNFMVCLTKGYPPQRKNIEIIDRTTRGI 240
           SELL S QL++V PIF VFLPH KFRKSSPGDPNFMVCLT+GYPP +K+IE ++RT+RGI
Sbjct: 181 SELLSSIQLDQVMPIFDVFLPHSKFRKSSPGDPNFMVCLTRGYPPPKKDIEFLERTSRGI 240

Query: 241 PMKYCHVEHGRVCFFSLDKVELPILP 266
            +KYCHVEHGRVCFFS DK+ELP+LP
Sbjct: 241 HIKYCHVEHGRVCFFSFDKLELPVLP 265

BLAST of Tan0007977 vs. NCBI nr
Match: XP_023538818.1 (tRNA-splicing endonuclease subunit Sen54-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 449.5 bits (1155), Expect = 2.0e-122
Identity = 216/266 (81.20%), Postives = 242/266 (90.98%), Query Frame = 0

Query: 1   MEPTDRE-SSSGASGDDDIYKQDIRDEEECLCSFGYMRKLQFRKHASTARWNDQMGMAEV 60
           ME TD E SS GAS DDD ++QDI++EEECLCS G MRKLQFRKHASTARWND+MGMAEV
Sbjct: 1   MEATDWEISSGGASDDDDNFEQDIKEEEECLCSSGNMRKLQFRKHASTARWNDEMGMAEV 60

Query: 61  VENKGNLWTTTGIVRWGKIYCSIEETLFLIEVGALHLLDYDNLNLSLKDVYKKVAEGKNG 120
           +ENKG+LWTT+GIVR GKIYCSIEETLFLIEVGALHLLD+DN +LSLKDVYKKVAEGK+ 
Sbjct: 61  LENKGSLWTTSGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGKSE 120

Query: 121 CLWEQFEVYRHLKSLGFIVGKHKVPWSVKGVRNGNDISSRSSIFENKGATNFESEDERSI 180
           C+WEQFEVYRHLKSLG+IVGKHKVPWSVKG RNG DISSRSSI ENKG+T+F SEDE+SI
Sbjct: 121 CIWEQFEVYRHLKSLGYIVGKHKVPWSVKGARNGGDISSRSSI-ENKGSTDFGSEDEKSI 180

Query: 181 SELLDSTQLNEVTPIFYVFLPHRKFRKSSPGDPNFMVCLTKGYPPQRKNIEIIDRTTRGI 240
            EL+D+ QLNEVTPIF V+LPH KFRKSSPGDPNFMVCLT+GYPP + +IE+I+R + GI
Sbjct: 181 CELIDAIQLNEVTPIFDVYLPHSKFRKSSPGDPNFMVCLTRGYPPPKTDIEVIERKSGGI 240

Query: 241 PMKYCHVEHGRVCFFSLDKVELPILP 266
           PMKYCHVEHGRVCFFS DKVELP+LP
Sbjct: 241 PMKYCHVEHGRVCFFSFDKVELPVLP 265

BLAST of Tan0007977 vs. NCBI nr
Match: KAG7028441.1 (tRNA-splicing endonuclease subunit Sen54 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 448.0 bits (1151), Expect = 5.8e-122
Identity = 215/266 (80.83%), Postives = 242/266 (90.98%), Query Frame = 0

Query: 1   MEPTDRE-SSSGASGDDDIYKQDIRDEEECLCSFGYMRKLQFRKHASTARWNDQMGMAEV 60
           ME TD E SS GAS DDD ++QDI++EEECLCS G MRKLQFRKHASTARWND+MGMAEV
Sbjct: 1   MEATDWEISSGGASDDDDNFEQDIKEEEECLCSSGNMRKLQFRKHASTARWNDEMGMAEV 60

Query: 61  VENKGNLWTTTGIVRWGKIYCSIEETLFLIEVGALHLLDYDNLNLSLKDVYKKVAEGKNG 120
           +ENKG+LWTT+GIVR GKIYCSIEETLFLIEVGALHLLD+DN +LSLKDVYKKVAEGK+ 
Sbjct: 61  LENKGSLWTTSGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGKSE 120

Query: 121 CLWEQFEVYRHLKSLGFIVGKHKVPWSVKGVRNGNDISSRSSIFENKGATNFESEDERSI 180
           C+WEQFEVYRHLKSLG+IVGKHKVPWSVKG RNG DISS+SSI ENKG+T+F SEDE+SI
Sbjct: 121 CIWEQFEVYRHLKSLGYIVGKHKVPWSVKGARNGGDISSQSSI-ENKGSTDFGSEDEKSI 180

Query: 181 SELLDSTQLNEVTPIFYVFLPHRKFRKSSPGDPNFMVCLTKGYPPQRKNIEIIDRTTRGI 240
            EL+D+ QLNEVTPIF V+LPH KFRKSSPGDPNFMVCLT+GYPP + +IE+I+R + GI
Sbjct: 181 CELIDAIQLNEVTPIFDVYLPHSKFRKSSPGDPNFMVCLTRGYPPPKTDIEVIERKSGGI 240

Query: 241 PMKYCHVEHGRVCFFSLDKVELPILP 266
           PMKYCHVEHGRVCFFS DKVELP+LP
Sbjct: 241 PMKYCHVEHGRVCFFSFDKVELPVLP 265

BLAST of Tan0007977 vs. NCBI nr
Match: KAG6596967.1 (Chromatin assembly factor 1 subunit FAS1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 448.0 bits (1151), Expect = 5.8e-122
Identity = 215/266 (80.83%), Postives = 242/266 (90.98%), Query Frame = 0

Query: 1   MEPTDRE-SSSGASGDDDIYKQDIRDEEECLCSFGYMRKLQFRKHASTARWNDQMGMAEV 60
           ME TD E SS GAS DDD ++QDI++EEECLCS G MRKLQFRKHASTARWND+MGMAEV
Sbjct: 272 MEATDWEISSGGASDDDDNFEQDIKEEEECLCSSGNMRKLQFRKHASTARWNDEMGMAEV 331

Query: 61  VENKGNLWTTTGIVRWGKIYCSIEETLFLIEVGALHLLDYDNLNLSLKDVYKKVAEGKNG 120
           +ENKG+LWTT+GIVR GKIYCSIEETLFLIEVGALHLLD+DN +LSLKDVYKKVAEGK+ 
Sbjct: 332 LENKGSLWTTSGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGKSE 391

Query: 121 CLWEQFEVYRHLKSLGFIVGKHKVPWSVKGVRNGNDISSRSSIFENKGATNFESEDERSI 180
           C+WEQFEVYRHLKSLG+IVGKHKVPWSVKG RNG DISS+SSI ENKG+T+F SEDE+SI
Sbjct: 392 CIWEQFEVYRHLKSLGYIVGKHKVPWSVKGARNGGDISSQSSI-ENKGSTDFGSEDEKSI 451

Query: 181 SELLDSTQLNEVTPIFYVFLPHRKFRKSSPGDPNFMVCLTKGYPPQRKNIEIIDRTTRGI 240
            EL+D+ QLNEVTPIF V+LPH KFRKSSPGDPNFMVCLT+GYPP + +IE+I+R + GI
Sbjct: 452 CELIDAIQLNEVTPIFDVYLPHSKFRKSSPGDPNFMVCLTRGYPPPKTDIEVIERKSGGI 511

Query: 241 PMKYCHVEHGRVCFFSLDKVELPILP 266
           PMKYCHVEHGRVCFFS DKVELP+LP
Sbjct: 512 PMKYCHVEHGRVCFFSFDKVELPVLP 536

BLAST of Tan0007977 vs. NCBI nr
Match: XP_022940787.1 (tRNA-splicing endonuclease subunit Sen54 [Cucurbita moschata])

HSP 1 Score: 446.8 bits (1148), Expect = 1.3e-121
Identity = 214/266 (80.45%), Postives = 242/266 (90.98%), Query Frame = 0

Query: 1   MEPTDRE-SSSGASGDDDIYKQDIRDEEECLCSFGYMRKLQFRKHASTARWNDQMGMAEV 60
           ME TD E SS GAS DDD ++QDI++EEECLCS G MRKLQFRKHASTARWND+MGMAEV
Sbjct: 1   MEATDWEISSGGASDDDDNFEQDIKEEEECLCSSGNMRKLQFRKHASTARWNDEMGMAEV 60

Query: 61  VENKGNLWTTTGIVRWGKIYCSIEETLFLIEVGALHLLDYDNLNLSLKDVYKKVAEGKNG 120
           +ENKG+LWTT+GIVR GKIYCSIEETLFLIEVGALHLLD+DN +LSLKDVYKKVAEGK+ 
Sbjct: 61  LENKGSLWTTSGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGKSE 120

Query: 121 CLWEQFEVYRHLKSLGFIVGKHKVPWSVKGVRNGNDISSRSSIFENKGATNFESEDERSI 180
           C+WEQFEVYRHLKSLG+IVGKHKVPWSVKG +NG DISS+SSI ENKG+T+F SEDE+SI
Sbjct: 121 CIWEQFEVYRHLKSLGYIVGKHKVPWSVKGAKNGGDISSQSSI-ENKGSTDFGSEDEKSI 180

Query: 181 SELLDSTQLNEVTPIFYVFLPHRKFRKSSPGDPNFMVCLTKGYPPQRKNIEIIDRTTRGI 240
            EL+D+ QLNEVTPIF V+LPH KFRKSSPGDPNFMVCLT+GYPP + +IE+I+R + GI
Sbjct: 181 CELIDAIQLNEVTPIFDVYLPHSKFRKSSPGDPNFMVCLTRGYPPPKTDIEVIERKSGGI 240

Query: 241 PMKYCHVEHGRVCFFSLDKVELPILP 266
           PMKYCHVEHGRVCFFS DKVELP+LP
Sbjct: 241 PMKYCHVEHGRVCFFSFDKVELPVLP 265

BLAST of Tan0007977 vs. ExPASy TrEMBL
Match: A0A6J1DRN3 (uncharacterized protein LOC111023660 OS=Momordica charantia OX=3673 GN=LOC111023660 PE=3 SV=1)

HSP 1 Score: 453.4 bits (1165), Expect = 6.7e-124
Identity = 218/266 (81.95%), Postives = 244/266 (91.73%), Query Frame = 0

Query: 1   MEPTDRESSS-GASGDDDIYKQDIRDEEECLCSFGYMRKLQFRKHASTARWNDQMGMAEV 60
           ME TD ESSS GASGDD+IY+QD+ DEEECLC+ G MRKLQFRKHASTARWNDQMGMAEV
Sbjct: 1   MEATDWESSSGGASGDDEIYEQDVEDEEECLCASGNMRKLQFRKHASTARWNDQMGMAEV 60

Query: 61  VENKGNLWTTTGIVRWGKIYCSIEETLFLIEVGALHLLDYDNLNLSLKDVYKKVAEGKNG 120
           +EN+G+LWTTTGIVR GKIYCS EETLFL+EVGALHLLD+DN +LSLKDVYKKVAEGKNG
Sbjct: 61  LENRGSLWTTTGIVRCGKIYCSFEETLFLMEVGALHLLDHDNSSLSLKDVYKKVAEGKNG 120

Query: 121 CLWEQFEVYRHLKSLGFIVGKHKVPWSVKGVRNGNDISSRSSIFENKGATNFESEDERSI 180
           CLWEQFEVYRHLKSLGFIVGKHKVPWSVKGVRNG+DIS +SSI EN+GA + ES+DERSI
Sbjct: 121 CLWEQFEVYRHLKSLGFIVGKHKVPWSVKGVRNGSDISPQSSI-ENEGAWDLESKDERSI 180

Query: 181 SELLDSTQLNEVTPIFYVFLPHRKFRKSSPGDPNFMVCLTKGYPPQRKNIEIIDRTTRGI 240
           SELL S QL++V PIF VFLPH KFRKSSPGDPNFMVCLT+GYPP +K+IE ++RT+RGI
Sbjct: 181 SELLSSIQLDQVMPIFDVFLPHSKFRKSSPGDPNFMVCLTRGYPPPKKDIEFLERTSRGI 240

Query: 241 PMKYCHVEHGRVCFFSLDKVELPILP 266
            +KYCHVEHGRVCFFS DK+ELP+LP
Sbjct: 241 HIKYCHVEHGRVCFFSFDKLELPVLP 265

BLAST of Tan0007977 vs. ExPASy TrEMBL
Match: A0A6J1FKK4 (tRNA-splicing endonuclease subunit Sen54 OS=Cucurbita moschata OX=3662 GN=LOC111446255 PE=3 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 6.2e-122
Identity = 214/266 (80.45%), Postives = 242/266 (90.98%), Query Frame = 0

Query: 1   MEPTDRE-SSSGASGDDDIYKQDIRDEEECLCSFGYMRKLQFRKHASTARWNDQMGMAEV 60
           ME TD E SS GAS DDD ++QDI++EEECLCS G MRKLQFRKHASTARWND+MGMAEV
Sbjct: 1   MEATDWEISSGGASDDDDNFEQDIKEEEECLCSSGNMRKLQFRKHASTARWNDEMGMAEV 60

Query: 61  VENKGNLWTTTGIVRWGKIYCSIEETLFLIEVGALHLLDYDNLNLSLKDVYKKVAEGKNG 120
           +ENKG+LWTT+GIVR GKIYCSIEETLFLIEVGALHLLD+DN +LSLKDVYKKVAEGK+ 
Sbjct: 61  LENKGSLWTTSGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGKSE 120

Query: 121 CLWEQFEVYRHLKSLGFIVGKHKVPWSVKGVRNGNDISSRSSIFENKGATNFESEDERSI 180
           C+WEQFEVYRHLKSLG+IVGKHKVPWSVKG +NG DISS+SSI ENKG+T+F SEDE+SI
Sbjct: 121 CIWEQFEVYRHLKSLGYIVGKHKVPWSVKGAKNGGDISSQSSI-ENKGSTDFGSEDEKSI 180

Query: 181 SELLDSTQLNEVTPIFYVFLPHRKFRKSSPGDPNFMVCLTKGYPPQRKNIEIIDRTTRGI 240
            EL+D+ QLNEVTPIF V+LPH KFRKSSPGDPNFMVCLT+GYPP + +IE+I+R + GI
Sbjct: 181 CELIDAIQLNEVTPIFDVYLPHSKFRKSSPGDPNFMVCLTRGYPPPKTDIEVIERKSGGI 240

Query: 241 PMKYCHVEHGRVCFFSLDKVELPILP 266
           PMKYCHVEHGRVCFFS DKVELP+LP
Sbjct: 241 PMKYCHVEHGRVCFFSFDKVELPVLP 265

BLAST of Tan0007977 vs. ExPASy TrEMBL
Match: A0A6J1I8D9 (tRNA-splicing endonuclease subunit Sen54 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111471897 PE=3 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 4.0e-121
Identity = 214/266 (80.45%), Postives = 242/266 (90.98%), Query Frame = 0

Query: 1   MEPTDRE-SSSGASGDDDIYKQDIRDEEECLCSFGYMRKLQFRKHASTARWNDQMGMAEV 60
           ME TD E SS GAS DDD ++QDI++EEECL S G MRKLQFRKHASTARWND+MGMAEV
Sbjct: 1   MEATDWEISSGGASDDDDNFEQDIKEEEECLRSSGNMRKLQFRKHASTARWNDEMGMAEV 60

Query: 61  VENKGNLWTTTGIVRWGKIYCSIEETLFLIEVGALHLLDYDNLNLSLKDVYKKVAEGKNG 120
           +ENKG+LWTT+GIVR GKIYCSIEETLFLIEVGALHLLD+DN +LSLKDVYKKVAEGK+ 
Sbjct: 61  LENKGSLWTTSGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGKSE 120

Query: 121 CLWEQFEVYRHLKSLGFIVGKHKVPWSVKGVRNGNDISSRSSIFENKGATNFESEDERSI 180
           C+WEQFEVYRHLKSLG+IVGKHKVPWSVKG RNG DISSRSSI ENKG+T+FESEDE+SI
Sbjct: 121 CIWEQFEVYRHLKSLGYIVGKHKVPWSVKGARNGGDISSRSSI-ENKGSTDFESEDEKSI 180

Query: 181 SELLDSTQLNEVTPIFYVFLPHRKFRKSSPGDPNFMVCLTKGYPPQRKNIEIIDRTTRGI 240
            ELL++ QLNE+TPIF V+LPH KFRKSSPGDPNFMVCLT+GYPP + +IE+I+R + GI
Sbjct: 181 CELLNAVQLNELTPIFDVYLPHSKFRKSSPGDPNFMVCLTRGYPPPKTDIEVIERKSGGI 240

Query: 241 PMKYCHVEHGRVCFFSLDKVELPILP 266
           PMKYCHVEHGRVCFFS DKV+LP+LP
Sbjct: 241 PMKYCHVEHGRVCFFSFDKVKLPVLP 265

BLAST of Tan0007977 vs. ExPASy TrEMBL
Match: A0A6J1ICR8 (tRNA-splicing endonuclease subunit Sen54 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111471897 PE=3 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 4.0e-121
Identity = 214/266 (80.45%), Postives = 242/266 (90.98%), Query Frame = 0

Query: 1   MEPTDRE-SSSGASGDDDIYKQDIRDEEECLCSFGYMRKLQFRKHASTARWNDQMGMAEV 60
           ME TD E SS GAS DDD ++QDI++EEECL S G MRKLQFRKHASTARWND+MGMAEV
Sbjct: 5   MEATDWEISSGGASDDDDNFEQDIKEEEECLRSSGNMRKLQFRKHASTARWNDEMGMAEV 64

Query: 61  VENKGNLWTTTGIVRWGKIYCSIEETLFLIEVGALHLLDYDNLNLSLKDVYKKVAEGKNG 120
           +ENKG+LWTT+GIVR GKIYCSIEETLFLIEVGALHLLD+DN +LSLKDVYKKVAEGK+ 
Sbjct: 65  LENKGSLWTTSGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGKSE 124

Query: 121 CLWEQFEVYRHLKSLGFIVGKHKVPWSVKGVRNGNDISSRSSIFENKGATNFESEDERSI 180
           C+WEQFEVYRHLKSLG+IVGKHKVPWSVKG RNG DISSRSSI ENKG+T+FESEDE+SI
Sbjct: 125 CIWEQFEVYRHLKSLGYIVGKHKVPWSVKGARNGGDISSRSSI-ENKGSTDFESEDEKSI 184

Query: 181 SELLDSTQLNEVTPIFYVFLPHRKFRKSSPGDPNFMVCLTKGYPPQRKNIEIIDRTTRGI 240
            ELL++ QLNE+TPIF V+LPH KFRKSSPGDPNFMVCLT+GYPP + +IE+I+R + GI
Sbjct: 185 CELLNAVQLNELTPIFDVYLPHSKFRKSSPGDPNFMVCLTRGYPPPKTDIEVIERKSGGI 244

Query: 241 PMKYCHVEHGRVCFFSLDKVELPILP 266
           PMKYCHVEHGRVCFFS DKV+LP+LP
Sbjct: 245 PMKYCHVEHGRVCFFSFDKVKLPVLP 269

BLAST of Tan0007977 vs. ExPASy TrEMBL
Match: A0A6J1I794 (tRNA-splicing endonuclease subunit Sen54 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111471897 PE=3 SV=1)

HSP 1 Score: 444.1 bits (1141), Expect = 4.0e-121
Identity = 214/266 (80.45%), Postives = 242/266 (90.98%), Query Frame = 0

Query: 1   MEPTDRE-SSSGASGDDDIYKQDIRDEEECLCSFGYMRKLQFRKHASTARWNDQMGMAEV 60
           ME TD E SS GAS DDD ++QDI++EEECL S G MRKLQFRKHASTARWND+MGMAEV
Sbjct: 15  MEATDWEISSGGASDDDDNFEQDIKEEEECLRSSGNMRKLQFRKHASTARWNDEMGMAEV 74

Query: 61  VENKGNLWTTTGIVRWGKIYCSIEETLFLIEVGALHLLDYDNLNLSLKDVYKKVAEGKNG 120
           +ENKG+LWTT+GIVR GKIYCSIEETLFLIEVGALHLLD+DN +LSLKDVYKKVAEGK+ 
Sbjct: 75  LENKGSLWTTSGIVRCGKIYCSIEETLFLIEVGALHLLDHDNSSLSLKDVYKKVAEGKSE 134

Query: 121 CLWEQFEVYRHLKSLGFIVGKHKVPWSVKGVRNGNDISSRSSIFENKGATNFESEDERSI 180
           C+WEQFEVYRHLKSLG+IVGKHKVPWSVKG RNG DISSRSSI ENKG+T+FESEDE+SI
Sbjct: 135 CIWEQFEVYRHLKSLGYIVGKHKVPWSVKGARNGGDISSRSSI-ENKGSTDFESEDEKSI 194

Query: 181 SELLDSTQLNEVTPIFYVFLPHRKFRKSSPGDPNFMVCLTKGYPPQRKNIEIIDRTTRGI 240
            ELL++ QLNE+TPIF V+LPH KFRKSSPGDPNFMVCLT+GYPP + +IE+I+R + GI
Sbjct: 195 CELLNAVQLNELTPIFDVYLPHSKFRKSSPGDPNFMVCLTRGYPPPKTDIEVIERKSGGI 254

Query: 241 PMKYCHVEHGRVCFFSLDKVELPILP 266
           PMKYCHVEHGRVCFFS DKV+LP+LP
Sbjct: 255 PMKYCHVEHGRVCFFSFDKVKLPVLP 279

BLAST of Tan0007977 vs. TAIR 10
Match: AT3G57360.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G02370.2); Has 122 Blast hits to 122 proteins in 54 species: Archae - 0; Bacteria - 0; Metazoa - 74; Fungi - 4; Plants - 41; Viruses - 0; Other Eukaryotes - 3 (source: NCBI BLink). )

HSP 1 Score: 213.8 bits (543), Expect = 1.7e-55
Identity = 106/265 (40.00%), Postives = 166/265 (62.64%), Query Frame = 0

Query: 1   MEPTDRESSSGASGDDDIYKQDIRDEEECLCSFGYMRKLQFRKHASTARWNDQMGMAEVV 60
           ME  D E+SS +  +         D++E   S G + KLQFR  +S ARW  ++GMAEV 
Sbjct: 1   MEEKDWEASSSSENEGGF----PNDDDEEFHSGGSVPKLQFRVGSSKARWITELGMAEVE 60

Query: 61  ENKGNLWTTTGIVRWGKIYCSIEETLFLIEVGALHLL-DYDNLNLSLKDVYKKVAEGKNG 120
             +G LWTTTGI+R GK YC IEE L+L E+G L +L + D++ + LKD+Y+K+AE K+G
Sbjct: 61  VKRGKLWTTTGIIRSGKTYCFIEEALYLSEIGELQILGNEDDIVIPLKDLYEKIAEEKSG 120

Query: 121 CLWEQFEVYRHLKSLGFIVGKHKVPWSVKGVRNGNDISSRSSIFENKGATNFESEDERSI 180
           C WE +EVYR+LK LG+I+G+H V W++K        ++R +  E          D  ++
Sbjct: 121 CSWENYEVYRYLKGLGYILGRHGVSWTLKD-------AARPNGEEESACAGECPADNDTV 180

Query: 181 SELLDSTQLNEVTPIFYVFLPHRKFRKSSPGDPNFMVCLTKGYPPQRKNIEIIDRTTRGI 240
           ++LL   Q+ +   +F V+LP+ +F+KSSPG+P+F+ C +   PP +++I+++ +     
Sbjct: 181 TKLLGDMQICDAKAVFDVYLPNSRFKKSSPGEPSFVACFSGDSPPSKEDIKVLQKRI-AA 240

Query: 241 PMKYCHVEHGRVCFFSLDKVELPIL 265
           P+ +CH+  GR  FFS   ++LP+L
Sbjct: 241 PLTFCHIAQGRASFFSFSSIDLPVL 253

BLAST of Tan0007977 vs. TAIR 10
Match: AT3G02370.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G57360.1). )

HSP 1 Score: 169.5 bits (428), Expect = 3.7e-42
Identity = 83/176 (47.16%), Postives = 120/176 (68.18%), Query Frame = 0

Query: 56  MAEVVENKGNLWTTTGIVRWGKIYCSIEETLFLIEVGALHLL-DYDNLNLSLKDVYKKVA 115
           MAEV   +G LWTTTGI+R GK YC IEE L+L E+G L LL D D++ +SLKD+Y ++A
Sbjct: 1   MAEVEVKRGKLWTTTGIIRTGKTYCFIEEALYLSEIGELQLLGDEDDVVISLKDLYGEIA 60

Query: 116 EGKNGCLWEQFEVYRHLKSLGFIVGKHKVPWSVKGVRNGNDISSRSSIFENKGATNFESE 175
           EGK GC WE +EVYR+LK LG+I+G+H VPW+ K   N    ++ S   E+  A  F  +
Sbjct: 61  EGKYGCCWENYEVYRYLKGLGYILGRHGVPWTTKYAVN----TTPSDEDESLCAAEF-FQ 120

Query: 176 DERSISELLDSTQLNEVTPIFYVFLPHRKFRKSSPGDPNFMVCLTKGYPPQRKNIE 231
           D  S+++LL    + +  P+F V+LP+ +F+KSSPG+P+F+ C +   PP ++ I+
Sbjct: 121 DRDSVTKLLSDMHICDARPVFDVYLPNSQFKKSSPGEPSFVTCFSGDSPPSKEEIK 171

BLAST of Tan0007977 vs. TAIR 10
Match: AT3G02370.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G57360.1); Has 95 Blast hits to 95 proteins in 40 species: Archae - 0; Bacteria - 0; Metazoa - 53; Fungi - 2; Plants - 39; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 162.5 bits (410), Expect = 4.5e-40
Identity = 80/165 (48.48%), Postives = 114/165 (69.09%), Query Frame = 0

Query: 56  MAEVVENKGNLWTTTGIVRWGKIYCSIEETLFLIEVGALHLL-DYDNLNLSLKDVYKKVA 115
           MAEV   +G LWTTTGI+R GK YC IEE L+L E+G L LL D D++ +SLKD+Y ++A
Sbjct: 1   MAEVEVKRGKLWTTTGIIRTGKTYCFIEEALYLSEIGELQLLGDEDDVVISLKDLYGEIA 60

Query: 116 EGKNGCLWEQFEVYRHLKSLGFIVGKHKVPWSVKGVRNGNDISSRSSIFENKGATNFESE 175
           EGK GC WE +EVYR+LK LG+I+G+H VPW+ K   N    ++ S   E+  A  F  +
Sbjct: 61  EGKYGCCWENYEVYRYLKGLGYILGRHGVPWTTKYAVN----TTPSDEDESLCAAEF-FQ 120

Query: 176 DERSISELLDSTQLNEVTPIFYVFLPHRKFRKSSPGDPNFMVCLT 220
           D  S+++LL    + +  P+F V+LP+ +F+KSSPG+P+F+ C +
Sbjct: 121 DRDSVTKLLSDMHICDARPVFDVYLPNSQFKKSSPGEPSFVTCFS 160

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O749084.2e-0635.58Probable tRNA-splicing endonuclease subunit sen54 OS=Schizosaccharomyces pombe (... [more]
Q74ZJ55.1e-0428.43tRNA-splicing endonuclease subunit SEN54 OS=Ashbya gossypii (strain ATCC 10895 /... [more]
Match NameE-valueIdentityDescription
XP_022156818.11.4e-12381.95uncharacterized protein LOC111023660 [Momordica charantia][more]
XP_023538818.12.0e-12281.20tRNA-splicing endonuclease subunit Sen54-like [Cucurbita pepo subsp. pepo][more]
KAG7028441.15.8e-12280.83tRNA-splicing endonuclease subunit Sen54 [Cucurbita argyrosperma subsp. argyrosp... [more]
KAG6596967.15.8e-12280.83Chromatin assembly factor 1 subunit FAS1, partial [Cucurbita argyrosperma subsp.... [more]
XP_022940787.11.3e-12180.45tRNA-splicing endonuclease subunit Sen54 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1DRN36.7e-12481.95uncharacterized protein LOC111023660 OS=Momordica charantia OX=3673 GN=LOC111023... [more]
A0A6J1FKK46.2e-12280.45tRNA-splicing endonuclease subunit Sen54 OS=Cucurbita moschata OX=3662 GN=LOC111... [more]
A0A6J1I8D94.0e-12180.45tRNA-splicing endonuclease subunit Sen54 isoform X3 OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1ICR84.0e-12180.45tRNA-splicing endonuclease subunit Sen54 isoform X2 OS=Cucurbita maxima OX=3661 ... [more]
A0A6J1I7944.0e-12180.45tRNA-splicing endonuclease subunit Sen54 isoform X1 OS=Cucurbita maxima OX=3661 ... [more]
Match NameE-valueIdentityDescription
AT3G57360.11.7e-5540.00unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
AT3G02370.23.7e-4247.16unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic... [more]
AT3G02370.14.5e-4048.48unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA... [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR024336tRNA-splicing endonuclease, subunit Sen54, N-terminalPFAMPF12928tRNA_int_end_N2coord: 40..96
e-value: 6.8E-11
score: 42.2
IPR024337tRNA-splicing endonuclease, subunit Sen54PANTHERPTHR21027TRNA-SPLICING ENDONUCLEASE SUBUNIT SEN54coord: 31..265

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0007977.1Tan0007977.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090305 nucleic acid phosphodiester bond hydrolysis
biological_process GO:0000379 tRNA-type intron splice site recognition and cleavage
cellular_component GO:0000214 tRNA-intron endonuclease complex
molecular_function GO:0004519 endonuclease activity