Tan0007728 (gene) Snake gourd v1

Overview
NameTan0007728
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionTHO complex subunit 3
LocationLG01: 19432254 .. 19436078 (-)
RNA-Seq ExpressionTan0007728
SyntenyTan0007728
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAAAAAGTGGGTCTTCTCAGCATAAAACAAAAATCCCTCTAAATCGCAGGATTTGAAATCGTTCAATTCCAGAAGGACATGGAGGAATCAGTGCCGGCTTTTAAAAATCTTCACAGCAGAGAGTATCAAGGTCACAAGAAGAAGGTTTTTTTTTTTCAAATCTCTTCTTCCTTCGTGCTACTCTCTATGGCGATTTCATTCTTTGTTTTAATCTTAAATCAATGTTTTCTTGTAACTTCGAAAGGTGCATTCTGTGGCATGGAATTGCACGGGTACGAAGCTTGCTTCCGGTTCTGTCGATCAAACTGCTCGAGTTTGGCATATTGAGCCTCACGGACATGTATGCTTTCTTATTACACTTTTTTTCCTCTCTCCTGGGGTTTAGCCAATTCGTTGTTTCTCTTGTGGAATCTTCTTCTCAAATTGCACGAATAGGTTTTTTTTTTTTTTTTGTTGGGGATACTCGATACTGTAAGTAGTTGAATTCGAGCTTCAGTCTTATCTTGAACTCTTCTATTCTGTTCTTCTCATATGGGTTTTCTTTTTCATCCGCCCGATATGATTAGAACATACACATTTGTCTCTTGCCCAAGTAGTGTTTGGAGTTTCTACTAGCTAGCTCAAGTACTGACATCTCTTCTCTCTCGCTGATTTTTTCTAGGCTGATAAAATTTAGCTGCATTTCCTATAGTTGATTACGTGAATGAAGTTATGGAGAGTTTTTTAATCCTTATACACACTGCCACACACACACACACATAAAGAAACTGGGTATGTGAGCTCCATCCTCGTGGCCTATCTTCGAGGCTTTGTCCATTTGGCCACTACAAGGCCAATTCCAAGAGACCAAAAAGATCTTAAGCTACTAAGGGAAGAGAATAAGCTTATGACCTTACTTGAACAGGATTTATTATAGGTTGTTCAACTGTATCTTTGAGTTCTATAAAAGGAAAGAGACTAAGCTTCTTACCTCCGGTTTGTATTCGTAGAATAATTGAGTTGGGGAAAGTTTTAACTTACAGGTTATGTTGCCTACTTCCTCTTTTTCCATATTGTTCCATAAGCTCTTGCGTTTTCCTTATTTTTAAGTTCTGGAAAATGGGGGATACAATTTGCTTAATTCTCTAGTTTTGTATACTTGTGAAAGCGTGTTATTGTTTGCTACCTCCTAATACCTGTTTTGATTTAATTCTGTGGTTTTGCTACGATGGTGCTCCACCATGACATTTAGTGTTGTCTTGTTTGTATGTATTTACTTACAACCCTCAACAAATCATTTCAATCACGTCAGCTGATGTTTGCTTCAGGGTAAGGTTAAGGATGTTGAGCTGAAAGGGCATACTGATAGTGTAGATCAGCTATGTTGGGATCCTAAACATTCTGATCTCATAGCAACTGCATCTGGGGACAAGACTGTTCGACTATGGGATGCTCGTAGTAAGTGTTTGAAACCGAGATTTGTGAAGTAACTGATCTAATCGAAGTTTAGAATTTGGTTCATAATTTCTCATTCTGTTCCATAGATGGGAAATGCTCACAGCAAGCTGAGCTCAGTGGGGAGAATATCAACATCACCTACAAACCTGATGGGACACACATAGCTGTTGGGAATAGGGTATGTTGTAGGGTGAAATTTGTGCATCATAAAATCAATTTAGATTTGTTATGCTTTAATGTACATTTCAAGATGTTTATATCCTCATGTAACTACTTGACCAATTCAATGCTTGCTGTTTTCTCACATCATTTTGCAGGATGATGAACTCACTATACTGGATGTTAGGAAGTTTAAGCCAGTCCACAAGCGCAAGTTCAACTACGAGGTAATCTTTATCTACAGTAAACAACAGTGATCACCCATTGGTCATATTTATGAAAACTTAAAAAGAGGAGCGTGTTAACTGTTAAGAAATTCAACAAATCACAACAGGACATAATAGCATACATGTGACTGCTTTGACATGTTTTGTTGATGTTGGTCTTCTTACCTTTATAAGAGAGGCTGTCTTTGCTCTATTCTTGAAAGCCCTTGATAGGTCCCTCTATATTAATGTGTATAGGAGGAAACATGTGAAGGGGAGGGACTAATGTAACATGTAAATAGTGTGATAAAATATGAACCCTTGTCGGGGTAATTAGTAACTAGTGTGTGGGGGGAGTTTATAGTAGTATGTACTAAGTCTAGTGGGTGGGGAGAGATAGGGAACTTTCTAATTAACCCTTAAGCTGTATTCTCAATATTCTTCAAATCTAGGGGATTCTATTCCATCAACCCCTTTACTATCTATACCCTCTTTCTTACCAGTTCTATTTGCTTTTTTTTTTCATGACTTTGTATGTTTTACTTTGACTATCTATATTGATAGATTTTTTTTATCATCTTTTCCAGGTGAATGAAATTGCTTGGAACATGACTGGGGAGATGTTTTTCCTGACAACTGGGAATGGTGAAGATCTGTAGCTATGACCTTTTAATGAAATGACCGAGGAGATTTTGTTGATTTCACTCTGTTGAATAAATTTTCATGTCTATTTTTTGTGCTCAGGTACTGTTGAAGTACTAGCATACCCATCGCTTCGACCAATAGAAACTCTTATGGCTCATACAGCTGGTTGTTACTGTATTGCAATTGACCCAGTTGGAGGGTGAGTTTCAAAATTGACTTCTTAACTTGAGTGAAGCTTCCATGATAAAAGAACTGTGTACAACTACTGCATCCAATATGCAGTCATGATTTCTTCTATTAAAAAAGACTGTCAGTTTTAATCTTTCAATTGCTTGCTGCAATTATTGGGAAAATTACTTCTGCAATTTAGGGAAAGATAAATTAAGTCCTTGTTACATCATAGGTACTTTGCTGTTGGAAGTGCTGATTCATTAGTTAGCCTATGGGATATCTCTCAGATGCTCTGCGTGCGAACATTTACAAAACTTGAGTAAGTTTTTTGTTTGATTTATACAACAATTTGTTTTATTCTTGCCTCGATACATTTCTTGTCCTGTCAATCGGAACACACTGGGAGCTTTGTAGTAGGTTTACAACACTGAATCATGTAGTGACTTTTCCTTTTCGTTCACTGAAGAATGTATTTTCTTGCCTCCTTTTTTTACTTCCTTAGATGGCCTGTCAGAACAATAAGTTTCAACCACACAGGAGAATACATTGCTTCTGCCAGTGAGGACTTGTTCATTGATATAGTAAGGATTCTAGCGCCTCTCCTTTCCTTATCTGATCTAGAATCTGGAACCTCCTTTTATTTGGCTGATGAGGATTAGTGATAAAACTGGTTCATGAATGGGGTGTTTTTCTTTTGGTTACAGTCAAATGTTCAAACAGGACGAACGGTTCATCAGATTCCTTGTCGGGCTGCAATGAACAGTGTGGAGTGGAATCCAAAGCACAATTTACTTGCATACGCTGGGGATGACAAGAACAAGTATCAGGCTGATGAAGGCAAGTTATCTGTGCATTGATATTTCCCACCGAACTTTTTAATTTCATGTATAGGAGCAGGAGGAGAGTTAAGGTTTTTACTTTCAAGACTTCACAAGTGTTATCTTTATTTTCTTGTTGCAGGTATTTTTAGGATCTTTGGGTTTGAAAGTGCATGAGAAATGGATCAACTATGAAGCAATCTCCATAGAATTCCAATGGATCAACTTATCTTTATTTTCTTCTTTTCTAGCTAATGTATTGAATTCCATTTGTAAGCTATGTTAGCGATATATATATATATATATATTTCTTTTGAAACAAGCTATGCTGGTGAATTTTCCCTTGAAACAACATACCGTGCATGTTGGAAAAAGTTCACCAGAA

mRNA sequence

AAAAAAAAAAGTGGGTCTTCTCAGCATAAAACAAAAATCCCTCTAAATCGCAGGATTTGAAATCGTTCAATTCCAGAAGGACATGGAGGAATCAGTGCCGGCTTTTAAAAATCTTCACAGCAGAGAGTATCAAGGTCACAAGAAGAAGGTGCATTCTGTGGCATGGAATTGCACGGGTACGAAGCTTGCTTCCGGTTCTGTCGATCAAACTGCTCGAGTTTGGCATATTGAGCCTCACGGACATGGTAAGGTTAAGGATGTTGAGCTGAAAGGGCATACTGATAGTGTAGATCAGCTATGTTGGGATCCTAAACATTCTGATCTCATAGCAACTGCATCTGGGGACAAGACTGTTCGACTATGGGATGCTCGTAATGGGAAATGCTCACAGCAAGCTGAGCTCAGTGGGGAGAATATCAACATCACCTACAAACCTGATGGGACACACATAGCTGTTGGGAATAGGGATGATGAACTCACTATACTGGATGTTAGGAAGTTTAAGCCAGTCCACAAGCGCAAGTTCAACTACGAGGTGAATGAAATTGCTTGGAACATGACTGGGGAGATGTTTTTCCTGACAACTGGGAATGGTACTGTTGAAGTACTAGCATACCCATCGCTTCGACCAATAGAAACTCTTATGGCTCATACAGCTGGTTGTTACTGTATTGCAATTGACCCAGTTGGAGGGTACTTTGCTGTTGGAAGTGCTGATTCATTAGTTAGCCTATGGGATATCTCTCAGATGCTCTGCGTGCGAACATTTACAAAACTTGAATGGCCTGTCAGAACAATAAGTTTCAACCACACAGGAGAATACATTGCTTCTGCCAGTGAGGACTTGTTCATTGATATATCAAATGTTCAAACAGGACGAACGGTTCATCAGATTCCTTGTCGGGCTGCAATGAACAGTGTGGAGTGGAATCCAAAGCACAATTTACTTGCATACGCTGGGGATGACAAGAACAAGTATCAGGCTGATGAAGGTATTTTTAGGATCTTTGGGTTTGAAAGTGCATGAGAAATGGATCAACTATGAAGCAATCTCCATAGAATTCCAATGGATCAACTTATCTTTATTTTCTTCTTTTCTAGCTAATGTATTGAATTCCATTTGTAAGCTATGTTAGCGATATATATATATATATATATTTCTTTTGAAACAAGCTATGCTGGTGAATTTTCCCTTGAAACAACATACCGTGCATGTTGGAAAAAGTTCACCAGAA

Coding sequence (CDS)

ATGGAGGAATCAGTGCCGGCTTTTAAAAATCTTCACAGCAGAGAGTATCAAGGTCACAAGAAGAAGGTGCATTCTGTGGCATGGAATTGCACGGGTACGAAGCTTGCTTCCGGTTCTGTCGATCAAACTGCTCGAGTTTGGCATATTGAGCCTCACGGACATGGTAAGGTTAAGGATGTTGAGCTGAAAGGGCATACTGATAGTGTAGATCAGCTATGTTGGGATCCTAAACATTCTGATCTCATAGCAACTGCATCTGGGGACAAGACTGTTCGACTATGGGATGCTCGTAATGGGAAATGCTCACAGCAAGCTGAGCTCAGTGGGGAGAATATCAACATCACCTACAAACCTGATGGGACACACATAGCTGTTGGGAATAGGGATGATGAACTCACTATACTGGATGTTAGGAAGTTTAAGCCAGTCCACAAGCGCAAGTTCAACTACGAGGTGAATGAAATTGCTTGGAACATGACTGGGGAGATGTTTTTCCTGACAACTGGGAATGGTACTGTTGAAGTACTAGCATACCCATCGCTTCGACCAATAGAAACTCTTATGGCTCATACAGCTGGTTGTTACTGTATTGCAATTGACCCAGTTGGAGGGTACTTTGCTGTTGGAAGTGCTGATTCATTAGTTAGCCTATGGGATATCTCTCAGATGCTCTGCGTGCGAACATTTACAAAACTTGAATGGCCTGTCAGAACAATAAGTTTCAACCACACAGGAGAATACATTGCTTCTGCCAGTGAGGACTTGTTCATTGATATATCAAATGTTCAAACAGGACGAACGGTTCATCAGATTCCTTGTCGGGCTGCAATGAACAGTGTGGAGTGGAATCCAAAGCACAATTTACTTGCATACGCTGGGGATGACAAGAACAAGTATCAGGCTGATGAAGGTATTTTTAGGATCTTTGGGTTTGAAAGTGCATGA

Protein sequence

MEESVPAFKNLHSREYQGHKKKVHSVAWNCTGTKLASGSVDQTARVWHIEPHGHGKVKDVELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDGTHIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPSLRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTISFNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQADEGIFRIFGFESA
Homology
BLAST of Tan0007728 vs. ExPASy Swiss-Prot
Match: Q9FKT5 (THO complex subunit 3 OS=Arabidopsis thaliana OX=3702 GN=THO3 PE=1 SV=1)

HSP 1 Score: 587.8 bits (1514), Expect = 7.3e-167
Identity = 273/315 (86.67%), Postives = 297/315 (94.29%), Query Frame = 0

Query: 1   MEESVPAFKNLHSREYQGHKKKVHSVAWNCTGTKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEE+   FK+LHSREYQGHKKKVHSVAWN  GTKLASGSVDQTAR+W+IEPHGH K KD+
Sbjct: 1   MEETTIPFKSLHSREYQGHKKKVHSVAWNSNGTKLASGSVDQTARIWNIEPHGHSKAKDL 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKHSDL+ATASGDK+VRLWDAR+GKC+QQ ELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHSDLVATASGDKSVRLWDARSGKCTQQVELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           TH+AVGNRDDELTILDVRKFKP+H+RKFNYEVNEIAWNM G+ FFLTTG GTVEVL+YPS
Sbjct: 121 THVAVGNRDDELTILDVRKFKPLHRRKFNYEVNEIAWNMPGDFFFLTTGLGTVEVLSYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           L+P++TL AHTAGCYCIAIDP G YFAVGSADSLVSLWDIS MLC+RTFTKLEWPVRTIS
Sbjct: 181 LKPLDTLTAHTAGCYCIAIDPKGRYFAVGSADSLVSLWDISDMLCLRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKN-KY 300
           FN++GEYIASASEDLFIDI+NVQTGRTVHQIPCRAAMNSVEWNPK+NLLAYAGDDKN KY
Sbjct: 241 FNYSGEYIASASEDLFIDIANVQTGRTVHQIPCRAAMNSVEWNPKYNLLAYAGDDKNPKY 300

Query: 301 QADEGIFRIFGFESA 315
             DEG+FRIFGFES+
Sbjct: 301 NTDEGVFRIFGFESS 315

BLAST of Tan0007728 vs. ExPASy Swiss-Prot
Match: Q29RH4 (THO complex subunit 3 OS=Bos taurus OX=9913 GN=THOC3 PE=2 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 1.3e-91
Identity = 154/301 (51.16%), Postives = 212/301 (70.43%), Query Frame = 0

Query: 13  SREYQGHKKKVHSVAWNCTGTKLASGSVDQTARVWHIEPHGHGKVKDVELKGHTDSVDQL 72
           +RE+  H  KVHSVAW+C G +LASGS D+TA V+ +E      VK+   +GH DSVDQL
Sbjct: 48  TREFPAHSAKVHSVAWSCDGRRLASGSFDKTASVFLLEK--DRLVKENNYRGHGDSVDQL 107

Query: 73  CWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDGTHIAVGNRDDEL 132
           CW P + DL  TASGDKT+R+WD R  KC       GENINI + PDG  IAVGN+DD +
Sbjct: 108 CWHPSNPDLFVTASGDKTIRIWDVRTTKCIATVNTKGENINICWSPDGQTIAVGNKDDVV 167

Query: 133 TILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPSLRPIETLMAHTA 192
           T +D +  +   + +F +EVNEI+WN    MFFLT GNG + +L+YP L+P++++ AH +
Sbjct: 168 TFIDAKTHRSKAEEQFKFEVNEISWNNDNNMFFLTNGNGCINILSYPELKPVQSINAHPS 227

Query: 193 GCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTISFNHTGEYIASAS 252
            C CI  DP+G YFA GSAD+LVSLWD+ +++CVR F++L+WPVRT+SF+H G+ +ASAS
Sbjct: 228 NCICIKFDPMGKYFATGSADALVSLWDVDELVCVRCFSRLDWPVRTLSFSHDGKMLASAS 287

Query: 253 EDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKN-KYQA--DEGIFRIF 311
           ED FIDI+ V+TG  + ++ C +   +V W+PK  LLA+A DDK+ KY +  + G  ++F
Sbjct: 288 EDHFIDIAEVETGDKLWEVQCESPTFTVAWHPKRPLLAFACDDKDGKYDSSREAGTVKLF 346

BLAST of Tan0007728 vs. ExPASy Swiss-Prot
Match: Q8VE80 (THO complex subunit 3 OS=Mus musculus OX=10090 GN=Thoc3 PE=2 SV=1)

HSP 1 Score: 337.8 bits (865), Expect = 1.3e-91
Identity = 154/301 (51.16%), Postives = 212/301 (70.43%), Query Frame = 0

Query: 13  SREYQGHKKKVHSVAWNCTGTKLASGSVDQTARVWHIEPHGHGKVKDVELKGHTDSVDQL 72
           +RE+  H  KVHSVAW+C G +LASGS D+TA V+ +E      VK+   +GH DSVDQL
Sbjct: 48  TREFPAHSAKVHSVAWSCDGRRLASGSFDKTASVFLLEK--DRLVKENNYRGHGDSVDQL 107

Query: 73  CWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDGTHIAVGNRDDEL 132
           CW P + DL  TASGDKT+R+WD R  KC       GENINI + PDG  IAVGN+DD +
Sbjct: 108 CWHPSNPDLFVTASGDKTIRIWDVRTTKCIATVNTKGENINICWSPDGQTIAVGNKDDVV 167

Query: 133 TILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPSLRPIETLMAHTA 192
           T +D +  +   + +F +EVNEI+WN    MFFLT GNG + +L+YP L+P++++ AH +
Sbjct: 168 TFIDAKTHRSKAEEQFKFEVNEISWNNDNNMFFLTNGNGCINILSYPELKPVQSINAHPS 227

Query: 193 GCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTISFNHTGEYIASAS 252
            C CI  DP+G YFA GSAD+LVSLWD+ +++CVR F++L+WPVRT+SF+H G+ +ASAS
Sbjct: 228 NCICIKFDPMGKYFATGSADALVSLWDVDELVCVRCFSRLDWPVRTLSFSHDGKMLASAS 287

Query: 253 EDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKN-KYQA--DEGIFRIF 311
           ED FIDI+ V+TG  + ++ C +   +V W+PK  LLA+A DDK+ KY +  + G  ++F
Sbjct: 288 EDHFIDIAEVETGDKLWEVQCESPTFTVAWHPKRPLLAFACDDKDGKYDSSREAGTVKLF 346

BLAST of Tan0007728 vs. ExPASy Swiss-Prot
Match: Q96J01 (THO complex subunit 3 OS=Homo sapiens OX=9606 GN=THOC3 PE=1 SV=1)

HSP 1 Score: 337.4 bits (864), Expect = 1.7e-91
Identity = 154/301 (51.16%), Postives = 212/301 (70.43%), Query Frame = 0

Query: 13  SREYQGHKKKVHSVAWNCTGTKLASGSVDQTARVWHIEPHGHGKVKDVELKGHTDSVDQL 72
           +RE+  H  KVHSVAW+C G +LASGS D+TA V+ +E      VK+   +GH DSVDQL
Sbjct: 48  TREFLAHSAKVHSVAWSCDGRRLASGSFDKTASVFLLEK--DRLVKENNYRGHGDSVDQL 107

Query: 73  CWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDGTHIAVGNRDDEL 132
           CW P + DL  TASGDKT+R+WD R  KC       GENINI + PDG  IAVGN+DD +
Sbjct: 108 CWHPSNPDLFVTASGDKTIRIWDVRTTKCIATVNTKGENINICWSPDGQTIAVGNKDDVV 167

Query: 133 TILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPSLRPIETLMAHTA 192
           T +D +  +   + +F +EVNEI+WN    MFFLT GNG + +L+YP L+P++++ AH +
Sbjct: 168 TFIDAKTHRSKAEEQFKFEVNEISWNNDNNMFFLTNGNGCINILSYPELKPVQSINAHPS 227

Query: 193 GCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTISFNHTGEYIASAS 252
            C CI  DP+G YFA GSAD+LVSLWD+ +++CVR F++L+WPVRT+SF+H G+ +ASAS
Sbjct: 228 NCICIKFDPMGKYFATGSADALVSLWDVDELVCVRCFSRLDWPVRTLSFSHDGKMLASAS 287

Query: 253 EDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKN-KYQA--DEGIFRIF 311
           ED FIDI+ V+TG  + ++ C +   +V W+PK  LLA+A DDK+ KY +  + G  ++F
Sbjct: 288 EDHFIDIAEVETGDKLWEVQCESPTFTVAWHPKRPLLAFACDDKDGKYDSSREAGTVKLF 346

BLAST of Tan0007728 vs. ExPASy Swiss-Prot
Match: P14197 (WD repeat-containing protein AAC3 OS=Dictyostelium discoideum OX=44689 GN=AAC3 PE=2 SV=2)

HSP 1 Score: 246.9 bits (629), Expect = 3.0e-64
Identity = 121/329 (36.78%), Postives = 195/329 (59.27%), Query Frame = 0

Query: 8   FKNLHSREYQGHKKKVHSVAWNCTGTKLASGSVDQTARVWHIEPHG-------------- 67
           F    ++++ G+KKK  SVAWN  GTK+AS   D   RVW+ +P G              
Sbjct: 153 FSECSTKDFIGNKKKSTSVAWNANGTKIASSGSDGIVRVWNFDPLGNSNNNNNSNNTSSN 212

Query: 68  --HGKVKD-VELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGE 127
             +  +K+ +ELKGH  S++++ W PK++DL+A+A  DK +++WD + GKC      + E
Sbjct: 213 SKNNNIKETIELKGHDGSIEKISWSPKNNDLLASAGTDKVIKIWDVKIGKCIGTVSTNSE 272

Query: 128 NINITYKPDGTHIAVGNRDDELTILDVRKFKPVHKRKFN-YEVNEIAWNMTGEMFFLTTG 187
           NI++ + PDG  I    RDD L ++D+   K +   KFN  E+N++ W+  G++  +   
Sbjct: 273 NIDVRWSPDGQFIVACTRDDHLALIDLPTIKTLKIYKFNGEELNQVGWDNNGDLILMANS 332

Query: 188 NGTVEVLAY-----PSLRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQML 247
            G +E   +       ++ ++TL  HTA  YC+  DP G Y A GSADS+VSLWDI  M+
Sbjct: 333 MGNIEAYKFLPKSTTHVKHLKTLYGHTASIYCMEFDPTGKYLAAGSADSIVSLWDIEDMM 392

Query: 248 CVRTFTKLEWPVRTISFNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNP 307
           CV+TF K  +P R++SF+  G++IA++S +  I+I ++++ + +H I C + ++S+ W+P
Sbjct: 393 CVKTFIKSTFPCRSVSFSFDGQFIAASSFESTIEIFHIESSQPIHTIEC-SGVSSLMWHP 452

Query: 308 KHNLLAYAGDDKNKYQADEGIFRIFGFES 314
              LLAYA  + N+   D  I R+FG+ S
Sbjct: 453 TLPLLAYA-PEINENNKDPSI-RVFGYHS 478

BLAST of Tan0007728 vs. NCBI nr
Match: XP_038905145.1 (THO complex subunit 3 [Benincasa hispida])

HSP 1 Score: 654.8 bits (1688), Expect = 3.7e-184
Identity = 309/314 (98.41%), Postives = 311/314 (99.04%), Query Frame = 0

Query: 1   MEESVPAFKNLHSREYQGHKKKVHSVAWNCTGTKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEES P FKNLHSREYQGHKKKVHSVAWNCTG KLASGSVDQTARVWHIEPHGHGKVKDV
Sbjct: 1   MEESAPIFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           THIAVGNRDDELTILDVRKFKP+HKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS
Sbjct: 121 THIAVGNRDDELTILDVRKFKPIHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS
Sbjct: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ
Sbjct: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300

Query: 301 ADEGIFRIFGFESA 315
           ADEGIFRIFGFE+A
Sbjct: 301 ADEGIFRIFGFETA 314

BLAST of Tan0007728 vs. NCBI nr
Match: XP_004149572.1 (THO complex subunit 3 [Cucumis sativus] >XP_008449067.1 PREDICTED: THO complex subunit 3 [Cucumis melo] >KGN56047.1 hypothetical protein Csa_011347 [Cucumis sativus])

HSP 1 Score: 653.7 bits (1685), Expect = 8.2e-184
Identity = 310/313 (99.04%), Postives = 310/313 (99.04%), Query Frame = 0

Query: 1   MEESVPAFKNLHSREYQGHKKKVHSVAWNCTGTKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEES  AFKNLHSREYQGHKKKVHSVAWNCTG KLASGSVDQTARVWHIEPHGHGKVKDV
Sbjct: 1   MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS
Sbjct: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS
Sbjct: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ
Sbjct: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300

Query: 301 ADEGIFRIFGFES 314
           ADEGIFRIFGFES
Sbjct: 301 ADEGIFRIFGFES 313

BLAST of Tan0007728 vs. NCBI nr
Match: XP_022946738.1 (THO complex subunit 3 [Cucurbita moschata] >XP_023005479.1 THO complex subunit 3 [Cucurbita maxima] >XP_023540495.1 THO complex subunit 3 [Cucurbita pepo subsp. pepo] >KAG6596748.1 THO complex subunit 3, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028285.1 THO complex subunit 3 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 652.5 bits (1682), Expect = 1.8e-183
Identity = 309/314 (98.41%), Postives = 312/314 (99.36%), Query Frame = 0

Query: 1   MEESVPAFKNLHSREYQGHKKKVHSVAWNCTGTKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEES PAFKNL+SREYQGHKKKVHSVAWNCTG KLASGSVDQTARVWHIEPHGHGKVKDV
Sbjct: 1   MEESTPAFKNLYSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS
Sbjct: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS
Sbjct: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FNHTGEYIASASEDLFIDIS+VQ+GRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ
Sbjct: 241 FNHTGEYIASASEDLFIDISSVQSGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300

Query: 301 ADEGIFRIFGFESA 315
           ADEGIFRIFGFESA
Sbjct: 301 ADEGIFRIFGFESA 314

BLAST of Tan0007728 vs. NCBI nr
Match: XP_022155009.1 (THO complex subunit 3 isoform X2 [Momordica charantia])

HSP 1 Score: 637.1 bits (1642), Expect = 8.0e-179
Identity = 300/314 (95.54%), Postives = 307/314 (97.77%), Query Frame = 0

Query: 1   MEESVPAFKNLHSREYQGHKKKVHSVAWNCTGTKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEE+   FKNL SREYQGHKKKVHSVAWNCTG KLASGSVDQTARVWHIEPHGHGKVKDV
Sbjct: 1   MEEAALPFKNLQSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDAR+GKCSQQ ELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARSGKCSQQVELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           TH+AVGNRDDELTILDVRKFKP+HKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS
Sbjct: 121 THVAVGNRDDELTILDVRKFKPIHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRP+ETLMAHTAGCYCIAIDPVG YFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS
Sbjct: 181 LRPLETLMAHTAGCYCIAIDPVGAYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FN+TG+YIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ
Sbjct: 241 FNYTGKYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300

Query: 301 ADEGIFRIFGFESA 315
           ADEGIFRIFGFESA
Sbjct: 301 ADEGIFRIFGFESA 314

BLAST of Tan0007728 vs. NCBI nr
Match: KAF8031774.1 (hypothetical protein BT93_D0862 [Corymbia citriodora subsp. variegata])

HSP 1 Score: 636.7 bits (1641), Expect = 1.0e-178
Identity = 297/314 (94.59%), Postives = 311/314 (99.04%), Query Frame = 0

Query: 1   MEESVPAFKNLHSREYQGHKKKVHSVAWNCTGTKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEE++P FKNLHSREYQGHKKKVHSVAWNCTGTKLASGSVDQTARVWHIEPHGHGKVKD+
Sbjct: 1   MEEAIP-FKNLHSREYQGHKKKVHSVAWNCTGTKLASGSVDQTARVWHIEPHGHGKVKDI 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKH+DLIATASGDKTVRLWDAR+GKCSQQAELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHADLIATASGDKTVRLWDARSGKCSQQAELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           TH+AVGNRDDELTILDVRKFKP+HKRKFNYEVNEIAWN +GEMFFLTTGNGTVEVLAYPS
Sbjct: 121 THVAVGNRDDELTILDVRKFKPIHKRKFNYEVNEIAWNTSGEMFFLTTGNGTVEVLAYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRP++TLMAHTAGCYCIAIDPVG YFAVGSADSLVSLWDIS+MLCVRTFTKLEWPVRTIS
Sbjct: 181 LRPVDTLMAHTAGCYCIAIDPVGRYFAVGSADSLVSLWDISEMLCVRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FNHTG+YIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPK+NLLAYAGDDKNKYQ
Sbjct: 241 FNHTGDYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKYNLLAYAGDDKNKYQ 300

Query: 301 ADEGIFRIFGFESA 315
           ADEG+FRIFGFESA
Sbjct: 301 ADEGVFRIFGFESA 313

BLAST of Tan0007728 vs. ExPASy TrEMBL
Match: A0A0A0L651 (WD_REPEATS_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G061010 PE=4 SV=1)

HSP 1 Score: 653.7 bits (1685), Expect = 4.0e-184
Identity = 310/313 (99.04%), Postives = 310/313 (99.04%), Query Frame = 0

Query: 1   MEESVPAFKNLHSREYQGHKKKVHSVAWNCTGTKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEES  AFKNLHSREYQGHKKKVHSVAWNCTG KLASGSVDQTARVWHIEPHGHGKVKDV
Sbjct: 1   MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS
Sbjct: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS
Sbjct: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ
Sbjct: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300

Query: 301 ADEGIFRIFGFES 314
           ADEGIFRIFGFES
Sbjct: 301 ADEGIFRIFGFES 313

BLAST of Tan0007728 vs. ExPASy TrEMBL
Match: A0A1S3BM38 (THO complex subunit 3 OS=Cucumis melo OX=3656 GN=LOC103491048 PE=4 SV=1)

HSP 1 Score: 653.7 bits (1685), Expect = 4.0e-184
Identity = 310/313 (99.04%), Postives = 310/313 (99.04%), Query Frame = 0

Query: 1   MEESVPAFKNLHSREYQGHKKKVHSVAWNCTGTKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEES  AFKNLHSREYQGHKKKVHSVAWNCTG KLASGSVDQTARVWHIEPHGHGKVKDV
Sbjct: 1   MEESAQAFKNLHSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS
Sbjct: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS
Sbjct: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ
Sbjct: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300

Query: 301 ADEGIFRIFGFES 314
           ADEGIFRIFGFES
Sbjct: 301 ADEGIFRIFGFES 313

BLAST of Tan0007728 vs. ExPASy TrEMBL
Match: A0A6J1KZA7 (THO complex subunit 3 OS=Cucurbita maxima OX=3661 GN=LOC111498453 PE=4 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 8.9e-184
Identity = 309/314 (98.41%), Postives = 312/314 (99.36%), Query Frame = 0

Query: 1   MEESVPAFKNLHSREYQGHKKKVHSVAWNCTGTKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEES PAFKNL+SREYQGHKKKVHSVAWNCTG KLASGSVDQTARVWHIEPHGHGKVKDV
Sbjct: 1   MEESTPAFKNLYSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS
Sbjct: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS
Sbjct: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FNHTGEYIASASEDLFIDIS+VQ+GRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ
Sbjct: 241 FNHTGEYIASASEDLFIDISSVQSGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300

Query: 301 ADEGIFRIFGFESA 315
           ADEGIFRIFGFESA
Sbjct: 301 ADEGIFRIFGFESA 314

BLAST of Tan0007728 vs. ExPASy TrEMBL
Match: A0A6J1G4N9 (THO complex subunit 3 OS=Cucurbita moschata OX=3662 GN=LOC111450715 PE=4 SV=1)

HSP 1 Score: 652.5 bits (1682), Expect = 8.9e-184
Identity = 309/314 (98.41%), Postives = 312/314 (99.36%), Query Frame = 0

Query: 1   MEESVPAFKNLHSREYQGHKKKVHSVAWNCTGTKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEES PAFKNL+SREYQGHKKKVHSVAWNCTG KLASGSVDQTARVWHIEPHGHGKVKDV
Sbjct: 1   MEESTPAFKNLYSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS
Sbjct: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS
Sbjct: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FNHTGEYIASASEDLFIDIS+VQ+GRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ
Sbjct: 241 FNHTGEYIASASEDLFIDISSVQSGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300

Query: 301 ADEGIFRIFGFESA 315
           ADEGIFRIFGFESA
Sbjct: 301 ADEGIFRIFGFESA 314

BLAST of Tan0007728 vs. ExPASy TrEMBL
Match: A0A6J1DNX6 (THO complex subunit 3 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022151 PE=4 SV=1)

HSP 1 Score: 637.1 bits (1642), Expect = 3.9e-179
Identity = 300/314 (95.54%), Postives = 307/314 (97.77%), Query Frame = 0

Query: 1   MEESVPAFKNLHSREYQGHKKKVHSVAWNCTGTKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEE+   FKNL SREYQGHKKKVHSVAWNCTG KLASGSVDQTARVWHIEPHGHGKVKDV
Sbjct: 1   MEEAALPFKNLQSREYQGHKKKVHSVAWNCTGMKLASGSVDQTARVWHIEPHGHGKVKDV 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDAR+GKCSQQ ELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARSGKCSQQVELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           TH+AVGNRDDELTILDVRKFKP+HKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS
Sbjct: 121 THVAVGNRDDELTILDVRKFKPIHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           LRP+ETLMAHTAGCYCIAIDPVG YFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS
Sbjct: 181 LRPLETLMAHTAGCYCIAIDPVGAYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300
           FN+TG+YIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ
Sbjct: 241 FNYTGKYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKNKYQ 300

Query: 301 ADEGIFRIFGFESA 315
           ADEGIFRIFGFESA
Sbjct: 301 ADEGIFRIFGFESA 314

BLAST of Tan0007728 vs. TAIR 10
Match: AT5G56130.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 587.8 bits (1514), Expect = 5.2e-168
Identity = 273/315 (86.67%), Postives = 297/315 (94.29%), Query Frame = 0

Query: 1   MEESVPAFKNLHSREYQGHKKKVHSVAWNCTGTKLASGSVDQTARVWHIEPHGHGKVKDV 60
           MEE+   FK+LHSREYQGHKKKVHSVAWN  GTKLASGSVDQTAR+W+IEPHGH K KD+
Sbjct: 1   MEETTIPFKSLHSREYQGHKKKVHSVAWNSNGTKLASGSVDQTARIWNIEPHGHSKAKDL 60

Query: 61  ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGENINITYKPDG 120
           ELKGHTDSVDQLCWDPKHSDL+ATASGDK+VRLWDAR+GKC+QQ ELSGENINITYKPDG
Sbjct: 61  ELKGHTDSVDQLCWDPKHSDLVATASGDKSVRLWDARSGKCTQQVELSGENINITYKPDG 120

Query: 121 THIAVGNRDDELTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPS 180
           TH+AVGNRDDELTILDVRKFKP+H+RKFNYEVNEIAWNM G+ FFLTTG GTVEVL+YPS
Sbjct: 121 THVAVGNRDDELTILDVRKFKPLHRRKFNYEVNEIAWNMPGDFFFLTTGLGTVEVLSYPS 180

Query: 181 LRPIETLMAHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCVRTFTKLEWPVRTIS 240
           L+P++TL AHTAGCYCIAIDP G YFAVGSADSLVSLWDIS MLC+RTFTKLEWPVRTIS
Sbjct: 181 LKPLDTLTAHTAGCYCIAIDPKGRYFAVGSADSLVSLWDISDMLCLRTFTKLEWPVRTIS 240

Query: 241 FNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLAYAGDDKN-KY 300
           FN++GEYIASASEDLFIDI+NVQTGRTVHQIPCRAAMNSVEWNPK+NLLAYAGDDKN KY
Sbjct: 241 FNYSGEYIASASEDLFIDIANVQTGRTVHQIPCRAAMNSVEWNPKYNLLAYAGDDKNPKY 300

Query: 301 QADEGIFRIFGFESA 315
             DEG+FRIFGFES+
Sbjct: 301 NTDEGVFRIFGFESS 315

BLAST of Tan0007728 vs. TAIR 10
Match: AT5G67320.1 (WD-40 repeat family protein )

HSP 1 Score: 109.0 bits (271), Expect = 7.0e-24
Identity = 83/293 (28.33%), Postives = 134/293 (45.73%), Query Frame = 0

Query: 12  HSREYQGHKKK-VHSVAWNCTGTKLASGSVDQTARVWHIEPHGHGKVKDVELKGHTDSVD 71
           H++     K K V ++ WN  GT LA+GS D  AR+W +    +G++    L  H   + 
Sbjct: 315 HAKGKSNEKSKDVTTLDWNGEGTLLATGSCDGQARIWTL----NGELIST-LSKHKGPIF 374

Query: 72  QLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAEL-SGENINITYKPDGTHIAVGNRD 131
            L W+ K  D + T S D+T  +WD +  +  QQ E  SG  +++ ++ +    A  + D
Sbjct: 375 SLKWN-KKGDYLLTGSVDRTAVVWDVKAEEWKQQFEFHSGPTLDVDWR-NNVSFATSSTD 434

Query: 132 DELTILDVRKFKPVHK-RKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPSLRPIETLM 191
             + +  + + +P         EVN + W+ TG +    + + T ++        +  L 
Sbjct: 435 SMIYLCKIGETRPAKTFTGHQGEVNCVKWDPTGSLLASCSDDSTAKIWNIKQSTFVHDLR 494

Query: 192 AHTAGCYCIAIDPVGG---------YFAVGSADSLVSLWD--ISQMLCVRTFTKLEWPVR 251
            HT   Y I   P G            A  S DS V LWD  + +MLC  +F     PV 
Sbjct: 495 EHTKEIYTIRWSPTGPGTNNPNKQLTLASASFDSTVKLWDAELGKMLC--SFNGHREPVY 554

Query: 252 TISFNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRAAMNSVEWNPKHNLLA 291
           +++F+  GEYIAS S D  I I +++ G+ V        +  V WN + N +A
Sbjct: 555 SLAFSPNGEYIASGSLDKSIHIWSIKEGKIVKTYTGNGGIFEVCWNKEGNKIA 598

BLAST of Tan0007728 vs. TAIR 10
Match: AT3G49660.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 86.7 bits (213), Expect = 3.7e-17
Identity = 76/314 (24.20%), Postives = 137/314 (43.63%), Query Frame = 0

Query: 1   MEESVPAFKN----LHSREYQGHKKKVHSVAWNCTGTKLASGSVDQTARVWHIEPHGHGK 60
           M E +PA  +    +HS+    H + V SV ++  G  LAS S D+T R + I       
Sbjct: 1   MAEEIPATASFTPYVHSQTLTSHNRAVSSVKFSSDGRLLASASADKTIRTYTINTINDPI 60

Query: 61  VKDV-ELKGHTDSVDQLCWDPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGEN---I 120
            + V E  GH + +  + +    +  I +AS DKT++LWD   G  S    L G      
Sbjct: 61  AEPVQEFTGHENGISDVAFS-SDARFIVSASDDKTLKLWDVETG--SLIKTLIGHTNYAF 120

Query: 121 NITYKPDGTHIAVGNRDDELTILDVR-----KFKPVHKRKFNYEVNEIAWNMTGEMFFLT 180
            + + P    I  G+ D+ + I DV      K  P H    +  V  + +N  G +   +
Sbjct: 121 CVNFNPQSNMIVSGSFDETVRIWDVTTGKCLKVLPAH----SDPVTAVDFNRDGSLIVSS 180

Query: 181 TGNGTVEVLAYPSLRPIETLM-AHTAGCYCIAIDPVGGYFAVGSADSLVSLWDISQMLCV 240
           + +G   +    +   ++TL+         +   P G +  VG+ D+ + LW+IS    +
Sbjct: 181 SYDGLCRIWDSGTGHCVKTLIDDENPPVSFVRFSPNGKFILVGTLDNTLRLWNISSAKFL 240

Query: 241 RTFT---KLEWPVRTISFNHTGEYIASASEDLFIDISNVQTGRTVHQIPCRA-AMNSVEW 297
           +T+T     ++ + +      G+ I S SED  + +  + + + + ++      + +V  
Sbjct: 241 KTYTGHVNAQYCISSAFSVTNGKRIVSGSEDNCVHMWELNSKKLLQKLEGHTETVMNVAC 300

BLAST of Tan0007728 vs. TAIR 10
Match: AT5G25150.1 (TBP-associated factor 5 )

HSP 1 Score: 80.5 bits (197), Expect = 2.7e-15
Identity = 64/257 (24.90%), Postives = 104/257 (40.47%), Query Frame = 0

Query: 16  YQGHKKKVHSVAWNCTGTKLASGSVDQTARVWHIEPHGHGKVKDVE-LKGHTDSVDQLCW 75
           Y+GH   V    ++  G   AS S D+TAR+W ++     +++ +  + GH   VD + W
Sbjct: 456 YKGHNYPVWDAQFSPFGHYFASCSHDRTARIWSMD-----RIQPLRIMAGHLSDVDCVQW 515

Query: 76  DPKHSDLIATASGDKTVRLWDARNGKCSQQAELSGEN---INITYKPDGTHIAVGNRDDE 135
            P + + IAT S DKTVRLWD + G+C +     G     +++   PDG ++A G+ D  
Sbjct: 516 HP-NCNYIATGSSDKTVRLWDVQTGECVR--IFIGHRSMVLSLAMSPDGRYMASGDED-- 575

Query: 136 LTILDVRKFKPVHKRKFNYEVNEIAWNMTGEMFFLTTGNGTVEVLAYPSLRPIETLMAHT 195
                                                  GT+ +    + R I  LM H 
Sbjct: 576 ---------------------------------------GTIMMWDLSTARCITPLMGHN 635

Query: 196 AGCYCIAIDPVGGYFAVGSADSLVSLWDI----------------SQMLCVRTFTKLEWP 253
           +  + ++    G   A GSAD  V LWD+                +++  +RTF     P
Sbjct: 636 SCVWSLSYSGEGSLLASGSADCTVKLWDVTSSTKLTKAEEKNGNSNRLRSLRTFPTKSTP 663

BLAST of Tan0007728 vs. TAIR 10
Match: AT5G08390.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 73.6 bits (179), Expect = 3.3e-13
Identity = 37/160 (23.12%), Postives = 74/160 (46.25%), Query Frame = 0

Query: 152 VNEIAWNMTGEMFFLTTGNGTVEVLAYPSLRPIETLMAHTAGCYCIAIDPVGGYFAVGSA 211
           ++ + ++ +  +      +GT+++      + + TL  H + C  +   P G +FA GS 
Sbjct: 62  IDSVTFDASEGLVAAGAASGTIKLWDLEEAKVVRTLTGHRSNCVSVNFHPFGEFFASGSL 121

Query: 212 DSLVSLWDISQMLCVRTFTKLEWPVRTISFNHTGEYIASASEDLFIDISNVQTGRTVHQI 271
           D+ + +WDI +  C+ T+      V  + F   G +I S  ED  + + ++  G+ +H+ 
Sbjct: 122 DTNLKIWDIRKKGCIHTYKGHTRGVNVLRFTPDGRWIVSGGEDNVVKVWDLTAGKLLHEF 181

Query: 272 PC-RAAMNSVEWNPKHNLLAYAGDDKNKYQADEGIFRIFG 311
                 + S++++P   LLA    DK     D   F + G
Sbjct: 182 KSHEGKIQSLDFHPHEFLLATGSADKTVKFWDLETFELIG 221

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FKT57.3e-16786.67THO complex subunit 3 OS=Arabidopsis thaliana OX=3702 GN=THO3 PE=1 SV=1[more]
Q29RH41.3e-9151.16THO complex subunit 3 OS=Bos taurus OX=9913 GN=THOC3 PE=2 SV=1[more]
Q8VE801.3e-9151.16THO complex subunit 3 OS=Mus musculus OX=10090 GN=Thoc3 PE=2 SV=1[more]
Q96J011.7e-9151.16THO complex subunit 3 OS=Homo sapiens OX=9606 GN=THOC3 PE=1 SV=1[more]
P141973.0e-6436.78WD repeat-containing protein AAC3 OS=Dictyostelium discoideum OX=44689 GN=AAC3 P... [more]
Match NameE-valueIdentityDescription
XP_038905145.13.7e-18498.41THO complex subunit 3 [Benincasa hispida][more]
XP_004149572.18.2e-18499.04THO complex subunit 3 [Cucumis sativus] >XP_008449067.1 PREDICTED: THO complex s... [more]
XP_022946738.11.8e-18398.41THO complex subunit 3 [Cucurbita moschata] >XP_023005479.1 THO complex subunit 3... [more]
XP_022155009.18.0e-17995.54THO complex subunit 3 isoform X2 [Momordica charantia][more]
KAF8031774.11.0e-17894.59hypothetical protein BT93_D0862 [Corymbia citriodora subsp. variegata][more]
Match NameE-valueIdentityDescription
A0A0A0L6514.0e-18499.04WD_REPEATS_REGION domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G... [more]
A0A1S3BM384.0e-18499.04THO complex subunit 3 OS=Cucumis melo OX=3656 GN=LOC103491048 PE=4 SV=1[more]
A0A6J1KZA78.9e-18498.41THO complex subunit 3 OS=Cucurbita maxima OX=3661 GN=LOC111498453 PE=4 SV=1[more]
A0A6J1G4N98.9e-18498.41THO complex subunit 3 OS=Cucurbita moschata OX=3662 GN=LOC111450715 PE=4 SV=1[more]
A0A6J1DNX63.9e-17995.54THO complex subunit 3 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022151 ... [more]
Match NameE-valueIdentityDescription
AT5G56130.15.2e-16886.67Transducin/WD40 repeat-like superfamily protein [more]
AT5G67320.17.0e-2428.33WD-40 repeat family protein [more]
AT3G49660.13.7e-1724.20Transducin/WD40 repeat-like superfamily protein [more]
AT5G25150.12.7e-1524.90TBP-associated factor 5 [more]
AT5G08390.13.3e-1323.13Transducin/WD40 repeat-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR020472G-protein beta WD-40 repeatPRINTSPR00320GPROTEINBRPTcoord: 82..96
score: 43.08
coord: 206..220
score: 33.56
coord: 35..49
score: 38.84
IPR001680WD40 repeatSMARTSM00320WD40_4coord: 222..261
e-value: 1.5
score: 15.9
coord: 55..95
e-value: 2.6E-8
score: 43.6
coord: 264..302
e-value: 48.0
score: 6.4
coord: 139..177
e-value: 150.0
score: 3.3
coord: 180..219
e-value: 6.7E-8
score: 42.3
coord: 9..48
e-value: 1.4E-7
score: 41.2
IPR001680WD40 repeatPFAMPF00400WD40coord: 183..219
e-value: 0.024
score: 15.5
coord: 61..95
e-value: 2.7E-4
score: 21.7
coord: 14..47
e-value: 2.8E-6
score: 27.9
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 62..104
score: 15.554599
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 187..228
score: 12.01227
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 16..50
score: 13.482671
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3D2.130.10.10coord: 56..148
e-value: 1.2E-18
score: 69.5
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3D2.130.10.10coord: 149..309
e-value: 2.4E-29
score: 104.3
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3D2.130.10.10coord: 2..55
e-value: 1.3E-9
score: 39.6
IPR024977Anaphase-promoting complex subunit 4, WD40 domainPFAMPF12894ANAPC4_WD40coord: 234..283
e-value: 2.0E-4
score: 21.6
IPR040132TREX component Tex1/THOC3PANTHERPTHR22839THO COMPLEX SUBUNIT 3 THO3coord: 7..312
IPR019775WD40 repeat, conserved sitePROSITEPS00678WD_REPEATS_1coord: 82..96
NoneNo IPR availablePROSITEPS50294WD_REPEATS_REGIONcoord: 187..221
score: 9.54651
NoneNo IPR availablePROSITEPS50294WD_REPEATS_REGIONcoord: 16..50
score: 11.181047
NoneNo IPR availablePROSITEPS50294WD_REPEATS_REGIONcoord: 62..100
score: 12.103769
NoneNo IPR availableCDDcd00200WD40coord: 12..296
e-value: 1.00655E-42
score: 147.096
IPR036322WD40-repeat-containing domain superfamilySUPERFAMILY50978WD40 repeat-likecoord: 11..296

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0007728.1Tan0007728.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006406 mRNA export from nucleus
biological_process GO:0010267 production of ta-siRNAs involved in RNA interference
cellular_component GO:0000445 THO complex part of transcription export complex
molecular_function GO:0005509 calcium ion binding
molecular_function GO:0005515 protein binding