Tan0007429 (gene) Snake gourd v1

Overview
NameTan0007429
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionPectin lyase-like superfamily protein
LocationLG07: 7582122 .. 7586104 (+)
RNA-Seq ExpressionTan0007429
SyntenyTan0007429
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAACTCTTTCATCCATGGGTCTTCCTAAAAAATCCACACAAACCCATGTAATTTTCTTCCTCTTTTTCTCAAAATTTTATTTGAGTCCTCTGTTTTCTTTCGATTAATCAATGTGGTTTGCTTATTCTTCAAATCTTCTCTGATTTTTTCATTTGGGTTTTTGAAAATGATTTGTGGGGTTTTGTTCAAAACCCAATTCGTTCTGTTTTCTCAAGAACAATTTTTAGACCCTTTATTTTCTTCAGAAAATTGAAAATGTAATCGAAACAAAGACGATCAAATTTGTTCGCCAGATATGGGTTCTGTTACGAAAAAAATCCATTAATTTCTCGGGAAACGAACAGAAATTAAATCATTTTTTTTTCCGGCAGGTTACGGCGGCGAGAATCTCGGCCGTGATTGTATTGGCATTGGTGAGTTTGGCCACCGTTGATGGCAAAAAGCCGTGTCCGTTTTCGAACTATTTGGAATTTCCGGCGCTCAGTTGCCGGAAACACACGGCGGTGCTGACGCAGTTTGGCGGCGTCGGCGACGGAGTAACTTCAAATACGCACGCGTTCCGGCAAGCGGTGGAACATCTCAGCCCACTGGCGTTGGAAGGCGGCGCTCAGCTGATTGTGCCGCCGGGGAAGTGGCTGACCGGAAGTTTTAATCTGACCAGCCATTTCACACTTTTTCTTCATAAAGATGCTCAAATTCTTGCGTCCCAGGTTTTACCCTAAACTATTTAATACTACATATATGGAAATGAAATTATTTATATTTTTGTTGGCATTTTGATTTTAACTTTTTCTAATTTGTCACTAGTGGGATTTTATTTTTGTTTGACTGTGGATTATTGCAAATGTTGCTTTTTTAATTAGGGTATTTATTGTTTTCACTTTTTTGTTTTTTTAGTTGAAACATTTTGAGGCGGATGAAATTTAGGAATAATAATAGGTATTTATTGTTTTTACTTTTCAATTTTATTTTTATTTGGAAGTGAATTATTTTTTTTTTAAAAAAAAAATCTAGGGTGTTTAAAATTTTATCTTCTAAATTATTACTATTTTTTTAAATATATATATATAAACCCTAGATGGGTCTTCAATTAGACTTGAGAAGTACAAGTGTGAGGGTTTCTACCTCTCACATCTAGCTGATATTATAATGTCTCTAACTATTTTTCTTTTTTTATTTTAAATGTATGTTTTTTGTTAATTTATTATGTAAAATGGAGTTTTTATTTTTCTTTGGAAATGAATTATCTATATTTTAATTTTGGGTTATTTATGGTGTTTCAATTAAAGTATGACTTTTTTCCCTCTCATTTTTATAATTAAAATTAAGAAGTTTCAGAATATACCTATAAGCCTTTTTCATTATTATAAAATCATCCTTCCTATAACTTAATGAAAGAGAAATATATATAATTGAAGAAAAAAGCACATGGATTTTGGAATAACCAAAAATGTTGAAGACCATATTTGGAACTTTTTCCAAAAATAAAAGAATTGATTGTATAATTAAGAATTCACAATAGTTTAGGACAAAAAAGGATGAGTTGTTGTGGATTTTCCTAAAAATAAATTTTCTACTAATTGTATTAAAATTAATTTAAAAAAACTATATATGTTTAACCCAATTGGTTTTTCTTTTTCCTTCTTTTGAATGAAGTACAAGTGCTTAAAAAGCGATATTAATTATTTGTCTGAAATGGAAATAAAATATTCAAAGCATTGATGTTAATAAGGGATTTCTTTTTTTGACAGAGATGTTAATAATTGAATTGATGAGGTAATAAAAATGTTAATATTGACATTTTTTAAAACAAAAAATAAAGGGGTAGGTATTGATTGACTTCATTGATGGAGTCAAATTGAATAAATCTTGTGTGTTAAAATTTTTTTTTTAAAAAAAAGTGAGTCGAAATGTGTACTAAGTTTTTGACTAAAAATTTGAGGTTTGGTTTTGATTTTGATTTAAACCGAACCCGACCCAAGTCAGTGAAATTGGGTAGTGATTTGGTGCGGGTGAGAGTACTGAGAACTGAAAGAGTTTCATAATCCAACGGTGCAGGATGAATCAGAATGGCCACTTGTCGAGGTTCTCCCATCTTATGGGAGGGGAAGAGATGCTCCTGGTGGACGATTCAGCAGCCTCATCTTTGGAACCAATCTCACCGATGTTGTCATCACAGGTGATTATTTCTATATTTTTTTCCTTAGTTTTAAAAATTAAATTTCTTTGCCCAACTTTTTTTTAATAAAAGAGTTAGGTGTTACACAACATAAATAAGAAACAGCAAGTTTGATATTTTGTCTGATATCATTTTAGTTTTAGATTTTAGGCTTTGCTTGAAAACAATTTTTTTAAAAAAAATAAAAATATAAGTTATAATATCTACTTTTTACAGTTGGCTTCTTTCCTCACTTTCTCATTTAAAAAAAAACTAAAAAACACATCCTGACATTAAAAAAAGTTGTTGATAATTCATTATTTTAAATTTATGTTAGATTTCAAAGGAAGTTAGATTAAAAAAGAAGCAAAGAAAATTAGTAGTATATCATACAAAAAAGTACCAAACCGATCTTAGATTTTAAAATTCTCAATGTTATTATATACATTTTAATTATTATTATTTATAAATAAACTTCAGAGTTTAAAAATAAAAATATAAAATATAAAAAAAAAACAGTTATTAGAAACATGTTTTTATTATTAAAAAAAAAACAAACAGGTTCCCTTCGTTAAAAAAGGAATTTGATAAAGAATTAGTGACGTGGTGTAGGTAACAACGGTACGATCGACGGGCAGGGATCTTATTGGTGGAGCAAGTTCCGCAAAAACGAGCTCAACGTAACACGGCCGTACATGATCGAGATCATGTACTCTGATCAAATCCAGATCTCAAATCTCACTTTGGTCAACTCTCCCTCGTGGTTTGTCCACCCGATCTACAGCAGGTTTGACCATCACACCCTAGTCTCATCCGCACTTGATCCCAACCCCCATTTTTTTAATTTTTTTTTTTCCTGTTGGTGAAAAATAAACTAAAATAAAATAAAAATGCAGAAATGTGATCATTCAAGGGCTCACCATTCTCGCTCCGATCGACTCTCCCAACACCGACGGAATCGATCCAGGTTCCATTCTTCTTCTTCTTCTTCATCAAAACCCTAGGCCTTTTTTTCCTCATCAATCCCTCTCCCTAATTCTTCTTTCAATTTCACAGATTCCTGCATCAACACTCGTATCGAAGACTGCTTCATCGTCTCCGGCGACGACTGCATCGCCGTCAAGAGCGGATGGGACCAGTACGGTATCAAATTCGGAATGCCGACCGAGGACCTGCTCATCCGCCGCCTCACCTGCATCTCACCGGATTCCGCCGGAATCGCCCTCGGCAGCGAGATGTCCGGCGGCATCCGCAACGTCAGGATCGAAAACGTCACCGCAATCAACACTCAGTCCGCCGTCCGAATCAAAACCGCTCGCGGCAGAGGCGGATTCGTCAAGGACATTTTCGTCCGACGGATGTCACTATCGACGATGAAATACGTCTTCTGGATGACCGGAAACTACAAGTCGCATCCGGATGAGAAGTTCGACCCGAACGCGCTGCCGGAGATTAAGAACATTAATTACAGAGACGTGGTGGCGGAGAATGTGACTTACTCGGCCAGATTGGAGGGGATTTCTGGAGATACGTTCACGGATATTTGTATTTCGAATGTGAAGATCGGACTGGCGGCGAAGGCGAAGAAGTTGCAGTGGAACTGTACGGACGTCGAGGGATACAGTAGCGATGTGGTGCCGCCGCCGTGTGCTCCGCTGGCAAAGGGGGAGCCGAAAAGCGGCGGATGTTACTTTCCCGAGGACCGGCTGCCGATCGAGGACGTTAAGTTGAAGACTTGCTTTGTTCAAAATCCTGCCTCGTGAAGGCTTTGATTTTTTTTTTTCTTCTATGAAATAAATCGTAATTTGGGATTTAAGTTGTCC

mRNA sequence

AAAAACTCTTTCATCCATGGGTCTTCCTAAAAAATCCACACAAACCCATGTTACGGCGGCGAGAATCTCGGCCGTGATTGTATTGGCATTGGTGAGTTTGGCCACCGTTGATGGCAAAAAGCCGTGTCCGTTTTCGAACTATTTGGAATTTCCGGCGCTCAGTTGCCGGAAACACACGGCGGTGCTGACGCAGTTTGGCGGCGTCGGCGACGGAGTAACTTCAAATACGCACGCGTTCCGGCAAGCGGTGGAACATCTCAGCCCACTGGCGTTGGAAGGCGGCGCTCAGCTGATTGTGCCGCCGGGGAAGTGGCTGACCGGAAGTTTTAATCTGACCAGCCATTTCACACTTTTTCTTCATAAAGATGCTCAAATTCTTGCGTCCCAGGATGAATCAGAATGGCCACTTGTCGAGGTTCTCCCATCTTATGGGAGGGGAAGAGATGCTCCTGGTGGACGATTCAGCAGCCTCATCTTTGGAACCAATCTCACCGATGTTGTCATCACAGGTAACAACGGTACGATCGACGGGCAGGGATCTTATTGGTGGAGCAAGTTCCGCAAAAACGAGCTCAACGTAACACGGCCGTACATGATCGAGATCATGTACTCTGATCAAATCCAGATCTCAAATCTCACTTTGGTCAACTCTCCCTCGTGGTTTGTCCACCCGATCTACAGCAGAAATGTGATCATTCAAGGGCTCACCATTCTCGCTCCGATCGACTCTCCCAACACCGACGGAATCGATCCAGATTCCTGCATCAACACTCGTATCGAAGACTGCTTCATCGTCTCCGGCGACGACTGCATCGCCGTCAAGAGCGGATGGGACCAGTACGGTATCAAATTCGGAATGCCGACCGAGGACCTGCTCATCCGCCGCCTCACCTGCATCTCACCGGATTCCGCCGGAATCGCCCTCGGCAGCGAGATGTCCGGCGGCATCCGCAACGTCAGGATCGAAAACGTCACCGCAATCAACACTCAGTCCGCCGTCCGAATCAAAACCGCTCGCGGCAGAGGCGGATTCGTCAAGGACATTTTCGTCCGACGGATGTCACTATCGACGATGAAATACGTCTTCTGGATGACCGGAAACTACAAGTCGCATCCGGATGAGAAGTTCGACCCGAACGCGCTGCCGGAGATTAAGAACATTAATTACAGAGACGTGGTGGCGGAGAATGTGACTTACTCGGCCAGATTGGAGGGGATTTCTGGAGATACGTTCACGGATATTTGTATTTCGAATGTGAAGATCGGACTGGCGGCGAAGGCGAAGAAGTTGCAGTGGAACTGTACGGACGTCGAGGGATACAGTAGCGATGTGGTGCCGCCGCCGTGTGCTCCGCTGGCAAAGGGGGAGCCGAAAAGCGGCGGATGTTACTTTCCCGAGGACCGGCTGCCGATCGAGGACGTTAAGTTGAAGACTTGCTTTGTTCAAAATCCTGCCTCGTGAAGGCTTTGATTTTTTTTTTTCTTCTATGAAATAAATCGTAATTTGGGATTTAAGTTGTCC

Coding sequence (CDS)

ATGGGTCTTCCTAAAAAATCCACACAAACCCATGTTACGGCGGCGAGAATCTCGGCCGTGATTGTATTGGCATTGGTGAGTTTGGCCACCGTTGATGGCAAAAAGCCGTGTCCGTTTTCGAACTATTTGGAATTTCCGGCGCTCAGTTGCCGGAAACACACGGCGGTGCTGACGCAGTTTGGCGGCGTCGGCGACGGAGTAACTTCAAATACGCACGCGTTCCGGCAAGCGGTGGAACATCTCAGCCCACTGGCGTTGGAAGGCGGCGCTCAGCTGATTGTGCCGCCGGGGAAGTGGCTGACCGGAAGTTTTAATCTGACCAGCCATTTCACACTTTTTCTTCATAAAGATGCTCAAATTCTTGCGTCCCAGGATGAATCAGAATGGCCACTTGTCGAGGTTCTCCCATCTTATGGGAGGGGAAGAGATGCTCCTGGTGGACGATTCAGCAGCCTCATCTTTGGAACCAATCTCACCGATGTTGTCATCACAGGTAACAACGGTACGATCGACGGGCAGGGATCTTATTGGTGGAGCAAGTTCCGCAAAAACGAGCTCAACGTAACACGGCCGTACATGATCGAGATCATGTACTCTGATCAAATCCAGATCTCAAATCTCACTTTGGTCAACTCTCCCTCGTGGTTTGTCCACCCGATCTACAGCAGAAATGTGATCATTCAAGGGCTCACCATTCTCGCTCCGATCGACTCTCCCAACACCGACGGAATCGATCCAGATTCCTGCATCAACACTCGTATCGAAGACTGCTTCATCGTCTCCGGCGACGACTGCATCGCCGTCAAGAGCGGATGGGACCAGTACGGTATCAAATTCGGAATGCCGACCGAGGACCTGCTCATCCGCCGCCTCACCTGCATCTCACCGGATTCCGCCGGAATCGCCCTCGGCAGCGAGATGTCCGGCGGCATCCGCAACGTCAGGATCGAAAACGTCACCGCAATCAACACTCAGTCCGCCGTCCGAATCAAAACCGCTCGCGGCAGAGGCGGATTCGTCAAGGACATTTTCGTCCGACGGATGTCACTATCGACGATGAAATACGTCTTCTGGATGACCGGAAACTACAAGTCGCATCCGGATGAGAAGTTCGACCCGAACGCGCTGCCGGAGATTAAGAACATTAATTACAGAGACGTGGTGGCGGAGAATGTGACTTACTCGGCCAGATTGGAGGGGATTTCTGGAGATACGTTCACGGATATTTGTATTTCGAATGTGAAGATCGGACTGGCGGCGAAGGCGAAGAAGTTGCAGTGGAACTGTACGGACGTCGAGGGATACAGTAGCGATGTGGTGCCGCCGCCGTGTGCTCCGCTGGCAAAGGGGGAGCCGAAAAGCGGCGGATGTTACTTTCCCGAGGACCGGCTGCCGATCGAGGACGTTAAGTTGAAGACTTGCTTTGTTCAAAATCCTGCCTCGTGA

Protein sequence

MGLPKKSTQTHVTAARISAVIVLALVSLATVDGKKPCPFSNYLEFPALSCRKHTAVLTQFGGVGDGVTSNTHAFRQAVEHLSPLALEGGAQLIVPPGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWSKFRKNELNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPNTDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLLIRRLTCISPDSAGIALGSEMSGGIRNVRIENVTAINTQSAVRIKTARGRGGFVKDIFVRRMSLSTMKYVFWMTGNYKSHPDEKFDPNALPEIKNINYRDVVAENVTYSARLEGISGDTFTDICISNVKIGLAAKAKKLQWNCTDVEGYSSDVVPPPCAPLAKGEPKSGGCYFPEDRLPIEDVKLKTCFVQNPAS
Homology
BLAST of Tan0007429 vs. ExPASy Swiss-Prot
Match: A7PZL3 (Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1)

HSP 1 Score: 634.0 bits (1634), Expect = 1.4e-180
Identity = 296/433 (68.36%), Postives = 353/433 (81.52%), Query Frame = 0

Query: 43  LEFPALSCRKHTAVLTQFGGVGDGVTSNTHAFRQAVEHLSPLALEGGAQLIVPPGKWLTG 102
           LE+ A+SCR H+A L  FGGVGDG T NT AF+ AV  LS    EGGAQL VP GKWLTG
Sbjct: 51  LEYSAISCRAHSASLVDFGGVGDGQTLNTKAFQDAVSELSKYGSEGGAQLYVPAGKWLTG 110

Query: 103 SFNLTSHFTLFLHKDAQILASQDESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVV 162
           SF+LTSHFTLFLH+DA +LASQD S+WP+++ LPSYGRGRDA  GR++SLIFGTNLTDV+
Sbjct: 111 SFSLTSHFTLFLHRDAVLLASQDISQWPVIKPLPSYGRGRDAAAGRYTSLIFGTNLTDVI 170

Query: 163 ITGNNGTIDGQGSYWWSKFRKNELNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYS 222
           ITG+NGTIDGQG  WW +F   +L  TRPY+IE+MYS  IQISNLTL+NSPSW VHP+YS
Sbjct: 171 ITGDNGTIDGQGGLWWQRFHGGKLKYTRPYLIELMYSADIQISNLTLLNSPSWNVHPVYS 230

Query: 223 RNVIIQGLTILAPIDSPNTDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMP 282
           RN++IQG+TILAP+ SPNTDGI+PDSC NTRIEDC+IVSGDDC+AVKSGWD+YGI +GMP
Sbjct: 231 RNILIQGITILAPVRSPNTDGINPDSCTNTRIEDCYIVSGDDCVAVKSGWDEYGIAYGMP 290

Query: 283 TEDLLIRRLTCISPDSAGIALGSEMSGGIRNVRIENVTAINTQSAVRIKTARGRGGFVKD 342
           T+ L+IRRLTCISP SA IALGSEMSGGI++VR E++ AIN++S +RIKT  GRGG+VKD
Sbjct: 291 TKQLVIRRLTCISPYSAVIALGSEMSGGIQDVRAEDIVAINSESGIRIKTGIGRGGYVKD 350

Query: 343 IFVRRMSLSTMKYVFWMTGNYKSHPDEKFDPNALPEIKNINYRDVVAENVTYSARLEGIS 402
           I+VR M++ TMK+ FWMTGNY SH D  +DP A P I+ INYRD+VAENV+ +ARLEGI 
Sbjct: 351 IYVRGMTMKTMKWAFWMTGNYGSHADNHYDPKAFPVIQGINYRDMVAENVSMAARLEGIP 410

Query: 403 GDTFTDICISNVKIGLAAKAKKLQWNCTDVEGYSSDVVPPPCAPLA-KGEPKSGGCYFPE 462
            D FT ICISNV I LAAKAKK+ W CTDVEG SS V P PC+ L  +G  K+  C FP 
Sbjct: 411 SDPFTGICISNVTIHLAAKAKKVPWTCTDVEGISSGVTPTPCSTLPDQGPEKTSLCNFPA 470

Query: 463 DRLPIEDVKLKTC 475
           + LPI+ V+L+ C
Sbjct: 471 ESLPIDTVELQKC 483

BLAST of Tan0007429 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 8.9e-31
Identity = 123/428 (28.74%), Postives = 189/428 (44.16%), Query Frame = 0

Query: 34  KKPCPFSNY-LEFPALSCRKHTAVLTQFGGVGDGVTSNTHAFRQAVEHLSPLALEGGAQL 93
           KK   F N+ L F  +    +   +TQFG VGDGVT ++ AF +A E  +  +  G  Q 
Sbjct: 2   KKKTWFLNFSLFFLQIFTSSNALDVTQFGAVGDGVTDDSQAFLKAWE--AVCSGTGDGQF 61

Query: 94  IVPPGKW-------LTGSFNLTSHFTLFLHKDAQILASQDESEWPLVEVLPSYGRGRDAP 153
           +VP G           GS   T  F   L K                 V PS G  +   
Sbjct: 62  VVPAGMTFMLQPLKFQGSCKSTPVFVQMLGK----------------LVAPSKGNWK--- 121

Query: 154 GGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWSKFRKNELNVTRPYMIEIMYSDQIQIS 213
            G     I  T++  +VI G +G I+GQGS WW      E   +RP  ++    + +++S
Sbjct: 122 -GDKDQWILFTDIEGLVIEG-DGEINGQGSSWW------EHKGSRPTALKFRSCNNLRLS 181

Query: 214 NLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPNTDGIDPDSCINTRIEDCFIVSGDDC 273
            LT ++SP   +H      V I  L I AP  SPNTDGID  +  N  I+DC I +GDDC
Sbjct: 182 GLTHLDSPMAHIHISECNYVTISSLRINAPESSPNTDGIDVGASSNVVIQDCIIATGDDC 241

Query: 274 IAVKSGWDQYGIKFGMPTEDLLIRRLTCISPDSAGIALGSEMSGG----IRNVRIENVTA 333
           IA+ SG           T ++ I  + C      GI++GS    G    + NV ++N   
Sbjct: 242 IAINSG-----------TSNIHISGIDC--GPGHGISIGSLGKDGETATVENVCVQNCNF 301

Query: 334 INTQSAVRIKTARGRGGFVKDIFVRRMSLSTMKY-----VFWMTGNYKSHPDEKFDPNAL 393
             T +  RIKT +G  G+ + I    ++L  ++       F+  G+  +  D K   ++ 
Sbjct: 302 RGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGGDSDNAKDRK---SSA 361

Query: 394 PEIKNINYRDVVAENVT-YSARLEGISGDTFTDICISNVKIGLAA--KAKKLQWNCTDVE 442
            E+  + + + +  + + Y            T+I + ++KI  A+    +  Q  C +V 
Sbjct: 362 VEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSGSGQVAQGQCLNVR 384

BLAST of Tan0007429 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 127.5 bits (319), Expect = 4.1e-28
Identity = 114/411 (27.74%), Postives = 177/411 (43.07%), Query Frame = 0

Query: 59  QFGGVGDGVTSNTHAFRQAVEHLSPLALEGGAQLIVPPGKWLTGS---FNLTSHFTLFLH 118
           Q+G VGDG   +T AF       +    +  A L+VP  K    +   F       L   
Sbjct: 63  QYGAVGDGKHDSTEAFATT---WNAACKKASAVLLVPANKKFFVNNLVFRGPCQPHLSFK 122

Query: 119 KDAQILASQDESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGS 178
            D  I+A  D + W   ++   + +                 LTD  + G  G IDGQG 
Sbjct: 123 VDGTIVAQPDPARWKNSKIWLQFAQ-----------------LTDFNLMG-TGVIDGQGQ 182

Query: 179 YWWSKFRK--------NELNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVII 238
            WW+   K        N+ N  RP  I+I YS  + +  LTL+NSP + +       V I
Sbjct: 183 QWWAGQCKVVNGRTVCNDRN--RPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKI 242

Query: 239 QGLTILAPIDSPNTDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLL 298
           QGL I AP DSPNTDGID  +     IE C I +GDDCIA+ +G           + ++ 
Sbjct: 243 QGLKIKAPRDSPNTDGIDIFASKRFHIEKCVIGTGDDCIAIGTG-----------SSNIT 302

Query: 299 IRRLTCISPDSAGIALGS----EMSGGIRNVRIENVTAINTQSAVRIKTARGRGGFVKDI 358
           I+ L C      GI++GS         + +V +     I+TQ+ +RIKT +G  G    I
Sbjct: 303 IKDLIC--GPGHGISIGSLGRDNSRAEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYI 362

Query: 359 FVRRMSLSTMKYVFWMTGNYKSHPDEKFDPNALPEIKNINYRDVVAENVTYSARLEGISG 418
               + +   +    +   Y +      +  +  +I+ + Y+++   + T +A ++ +  
Sbjct: 363 TYENVEMINSENPILINQFYCTSASACQNQRSAVQIQGVTYKNIHGTSAT-AAAIQLMCS 422

Query: 419 DTF--TDICISNVKIGLAAKAKKLQWNCTD--VEGYSSDVVPPPCAPLAKG 451
           D+   T I +SNV + L +       +C D    G+ S  + P C  L  G
Sbjct: 423 DSVPCTGIQLSNVSLKLTSGKPA---SCVDKNARGFYSGRLIPTCKNLRPG 433

BLAST of Tan0007429 vs. ExPASy Swiss-Prot
Match: Q9FY19 (Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 4.5e-27
Identity = 113/409 (27.63%), Postives = 183/409 (44.74%), Query Frame = 0

Query: 60  FGGVGDGVTSNTHAFRQ----AVEHLSPLALEGGAQLIVPPGKWLTGSFNLTSHFTLFLH 119
           +G VGDG   +T AF +    A   LS + L    +  V       G      HF+  + 
Sbjct: 65  YGAVGDGKHDSTDAFEKTWNAACNKLSAVFLVPANKKFVVNNLVFYGP--CQPHFSFKV- 124

Query: 120 KDAQILASQDESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGS 179
            D  I A  D ++W   ++   + R                 LTD  + G  G IDGQG+
Sbjct: 125 -DGTIAAYPDPAKWKNSKIWMHFAR-----------------LTDFNLMG-TGVIDGQGN 184

Query: 180 YWWSKFRKNELNVT------RPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSR--NVII 239
            WWS   K     T      RP  I+I +S  + +  LTL NSP +  H ++     V I
Sbjct: 185 RWWSDQCKTINGRTVCNDKGRPTAIKIDFSKSVTVKELTLTNSPEF--HLVFGECDGVKI 244

Query: 240 QGLTILAPIDSPNTDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLL 299
           QG+ I AP DSPNTDGID  +     IE C I +GDDC+AV +G           + ++ 
Sbjct: 245 QGIKIKAPRDSPNTDGIDIFASKRFEIEKCTIGTGDDCVAVGTG-----------SSNIT 304

Query: 300 IRRLTCISPDSAGIALGSEMSGGIRN----VRIENVTAINTQSAVRIKTARGRGGFVKDI 359
           I+ LTC      G+++GS   G  R+    V ++    I+TQ+ +RIKT +G  G    I
Sbjct: 305 IKDLTC--GPGHGMSIGSLGKGNSRSEVSFVHLDGAKFIDTQNGLRIKTWQGGSGLASHI 364

Query: 360 FVRRMSLSTMKYVFWMTGNYKSHPDEKFDPNALPEIKNINYRDVVAENVTYSARLEGISG 419
               + +   +    +   Y +      +  +  +I+++ ++++   + T +A ++ +  
Sbjct: 365 TYENVEMINAENPILINQFYCTSAAACKNQRSAVKIQDVTFKNIHGTSAT-TAAIQLMCS 424

Query: 420 DTF--TDICISNVKIGLAAKAKKLQWNCTDVEGYSSDVVPPPCAPLAKG 451
           D+   ++I +SNV + L +  K       +  GY ++ + P C  L  G
Sbjct: 425 DSVPCSNIKLSNVFLKLTS-GKVATCVNKNANGYYTNPLNPSCKSLHPG 434

BLAST of Tan0007429 vs. ExPASy Swiss-Prot
Match: P27644 (Polygalacturonase OS=Rhizobium radiobacter OX=358 GN=pgl PE=2 SV=1)

HSP 1 Score: 110.5 bits (275), Expect = 5.2e-23
Identity = 71/221 (32.13%), Postives = 106/221 (47.96%), Query Frame = 0

Query: 194 IEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPNTDGIDPDSCINTR 253
           + ++   + Q+   T+ N+ SW +HP    ++     TI+AP DSPNTDG +P+SC N  
Sbjct: 25  LHLVSCHKTQLLGFTIRNAASWTIHPQGCEDLTAAASTIIAPHDSPNTDGFNPESCRNVM 84

Query: 254 IEDCFIVSGDDCIAVKSGWDQYGIKFGMPTED-------LLIRRLTCISPDSAGIALGSE 313
           I       GDDCIAVK+G      K G   ED        +  R   + P   G+ +GSE
Sbjct: 85  ISGVRFSVGDDCIAVKAG------KRGPDGEDDHLAETRGITVRHCLMQPGHGGLVIGSE 144

Query: 314 MSGGIRNVRIENVTAINTQSAVRIKT-ARGRGGFVKDIFVRRMSLSTMKYVFWMTGNYKS 373
           MSGG+ +V +E+   I T   +R+KT AR  GG V +I +RR+ L  ++       +Y  
Sbjct: 145 MSGGVHDVTVEDCDMIGTDRGLRLKTGARSGGGMVGNITMRRVLLDGVQTALSANAHYHC 204

Query: 374 HPDEKFD----------PNALPEIKNINYRDVVAENVTYSA 397
             D   D           +  P +  I   DV   N+ ++A
Sbjct: 205 DADGHDDWVQSRNPAPVNDGTPFVDGITVEDVEIRNLAHAA 239

BLAST of Tan0007429 vs. NCBI nr
Match: XP_038906749.1 (probable polygalacturonase [Benincasa hispida])

HSP 1 Score: 904.4 bits (2336), Expect = 4.1e-259
Identity = 435/481 (90.44%), Postives = 455/481 (94.59%), Query Frame = 0

Query: 1   MGLPKKSTQTHVTAARISAVIVLALVSLATVDGKKPCPFSNYLEFPALSCRKHTAVLTQF 60
           M LPK ST+ HVTAARISAVI+LALVSLATV+GKKPCP +NYLE PA+SCRKHTAVLT F
Sbjct: 1   MDLPKNSTKFHVTAARISAVIILALVSLATVEGKKPCPTTNYLEVPAISCRKHTAVLTDF 60

Query: 61  GGVGDGVTSNTHAFRQAVEHLSPLALEGGAQLIVPPGKWLTGSFNLTSHFTLFLHKDAQI 120
           GGVGDGVTSNTHAFRQA+EHLSPLA +GGAQLIVPPGKWLTGSFNLTSHFTLF+HK A I
Sbjct: 61  GGVGDGVTSNTHAFRQAIEHLSPLAADGGAQLIVPPGKWLTGSFNLTSHFTLFVHKYATI 120

Query: 121 LASQDESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWSK 180
           LASQDESEWPLV VLPSYGRGRDAPGGR+SSLIFGTNLTDVVITGNNGTIDGQGSYWW K
Sbjct: 121 LASQDESEWPLVAVLPSYGRGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGSYWWDK 180

Query: 181 FRKNELNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240
           F K+ELNVTRPY+IE+MYSDQIQIS+LTLVNSPSWFVHPIYSRNVII+GLTILAPIDSPN
Sbjct: 181 FHKDELNVTRPYLIEMMYSDQIQISDLTLVNSPSWFVHPIYSRNVIIRGLTILAPIDSPN 240

Query: 241 TDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLLIRRLTCISPDSAG 300
           TDGIDPDSC NTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDL+IRRLTCISPDSAG
Sbjct: 241 TDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAG 300

Query: 301 IALGSEMSGGIRNVRIENVTAINTQSAVRIKTARGRGGFVKDIFVRRMSLSTMKYVFWMT 360
           IALGSEMSGGIRNVRIENVT INTQSAVRIKTARGRGGFVKDIFVR M LSTMKYVFWMT
Sbjct: 301 IALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMT 360

Query: 361 GNYKSHPDEKFDPNALPEIKNINYRDVVAENVTYSARLEGISGDTFTDICISNVKIGLAA 420
           GNYKSHPD+KFDP A+PEI NINYRDVVAENVTYSARLEGISGD FTDICISNVKIGL A
Sbjct: 361 GNYKSHPDDKFDPTAVPEITNINYRDVVAENVTYSARLEGISGDPFTDICISNVKIGLTA 420

Query: 421 KAKKLQWNCTDVEGYSSDVVPPPCAPLAKGEPKSGGCYFPEDRLPIEDVKLKTCFVQNPA 480
           K KKLQWNCTDVEG+SSDV+PPPCAPLAK   KSGGCYFPED LPIE V+LKTCFVQNPA
Sbjct: 421 KPKKLQWNCTDVEGFSSDVMPPPCAPLAKA-AKSGGCYFPEDELPIEKVQLKTCFVQNPA 480

Query: 481 S 482
           S
Sbjct: 481 S 480

BLAST of Tan0007429 vs. NCBI nr
Match: XP_023532553.1 (probable polygalacturonase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 902.9 bits (2332), Expect = 1.2e-258
Identity = 434/480 (90.42%), Postives = 454/480 (94.58%), Query Frame = 0

Query: 1   MGLPKKSTQTHVTAARISAVIVLALVSLATVDGKKPCPFSNYLEFPALSCRKHTAVLTQF 60
           M LP K T+T +TAAR+SAVI+LA VSL +VDGKKPCPFS+YLEFPA+SCRKHTAVLT F
Sbjct: 1   MDLPHKPTKTQITAARVSAVILLAFVSLGSVDGKKPCPFSSYLEFPAISCRKHTAVLTDF 60

Query: 61  GGVGDGVTSNTHAFRQAVEHLSPLALEGGAQLIVPPGKWLTGSFNLTSHFTLFLHKDAQI 120
           GGVGDGVTSNT+AFR+A+EHLS LA++GGAQLIVPPGKWLTGSFNLTSHFTLF+HKDAQI
Sbjct: 61  GGVGDGVTSNTNAFREAMEHLSTLAVDGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAQI 120

Query: 121 LASQDESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWSK 180
           LASQDESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWW K
Sbjct: 121 LASQDESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWDK 180

Query: 181 FRKNELNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240
           F +NELNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN
Sbjct: 181 FHRNELNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240

Query: 241 TDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLLIRRLTCISPDSAG 300
           TDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGI+FGMPTEDLLIRRLTCISPDSAG
Sbjct: 241 TDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIQFGMPTEDLLIRRLTCISPDSAG 300

Query: 301 IALGSEMSGGIRNVRIENVTAINTQSAVRIKTARGRGGFVKDIFVRRMSLSTMKYVFWMT 360
           IALGSEMSGGIRNVRIENVTAINTQSAVRIKTA GRGG+VKDIFVR+M LSTMKYVFWMT
Sbjct: 301 IALGSEMSGGIRNVRIENVTAINTQSAVRIKTAIGRGGYVKDIFVRQMFLSTMKYVFWMT 360

Query: 361 GNYKSHPDEKFDPNALPEIKNINYRDVVAENVTYSARLEGISGDTFTDICISNVKIGLAA 420
           GNYKSHPDEKFDP ALPEIKNINYRDVVAENVTYSARL+GISGD FTDICISNVKIGL A
Sbjct: 361 GNYKSHPDEKFDPKALPEIKNINYRDVVAENVTYSARLDGISGDPFTDICISNVKIGLTA 420

Query: 421 KAKKLQWNCTDVEGYSSDVVPPPCAPLAKGEPKSGGCYFPEDRLPIEDVKLKTCFVQNPA 480
           K KKLQWNCT+VEGYSSDVVPPPCAPLAK    S GC FPEDRLPIEDVKL TC  +N A
Sbjct: 421 KPKKLQWNCTNVEGYSSDVVPPPCAPLAKEVVSSDGCPFPEDRLPIEDVKLMTCVTKNHA 480

BLAST of Tan0007429 vs. NCBI nr
Match: XP_022996001.1 (probable polygalacturonase [Cucurbita maxima])

HSP 1 Score: 895.2 bits (2312), Expect = 2.5e-256
Identity = 431/481 (89.60%), Postives = 452/481 (93.97%), Query Frame = 0

Query: 1   MGLPKKSTQTHVTAARISAVIVLALVSLATVDGKKPCPFSNYLEFPALSCRKHTAVLTQF 60
           M LP K T+T +TA RISAVI+L LVSLA+VDGKKPCPFS+YLEF A+SCRKHTAVLT F
Sbjct: 1   MDLPHKPTKTQITAVRISAVILLVLVSLASVDGKKPCPFSSYLEFSAISCRKHTAVLTDF 60

Query: 61  GGVGDGVTSNTHAFRQAVEHLSPLALEGGAQLIVPPGKWLTGSFNLTSHFTLFLHKDAQI 120
           GGVGDGVTSNT+AFR+A+EHLS LA++GG+QLIVPPGKWLTGSFNLTSHFTLF+HKDAQI
Sbjct: 61  GGVGDGVTSNTNAFREAIEHLSTLAIDGGSQLIVPPGKWLTGSFNLTSHFTLFVHKDAQI 120

Query: 121 LASQDESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWSK 180
           LASQDESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWW K
Sbjct: 121 LASQDESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWDK 180

Query: 181 FRKNELNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240
           F KNE NVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN
Sbjct: 181 FHKNEFNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240

Query: 241 TDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLLIRRLTCISPDSAG 300
           TDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGI+FGMPTEDLLIRRLTCISPDSAG
Sbjct: 241 TDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIQFGMPTEDLLIRRLTCISPDSAG 300

Query: 301 IALGSEMSGGIRNVRIENVTAINTQSAVRIKTARGRGGFVKDIFVRRMSLSTMKYVFWMT 360
           IALGSEMSGGIRNVRIENVTAINTQSAVRIKTA GRGG+VKDIFVR+M LSTMKYVFWMT
Sbjct: 301 IALGSEMSGGIRNVRIENVTAINTQSAVRIKTAIGRGGYVKDIFVRQMFLSTMKYVFWMT 360

Query: 361 GNYKSHPDEKFDPNALPEIKNINYRDVVAENVTYSARLEGISGDTFTDICISNVKIGLAA 420
           GNYKSHPDEKFDP ALPEIKNINYRDVVAENVTYSARL+GISGD FTDICISNVKIGL A
Sbjct: 361 GNYKSHPDEKFDPKALPEIKNINYRDVVAENVTYSARLDGISGDPFTDICISNVKIGLTA 420

Query: 421 KAKKLQWNCTDVEGYSSDVVPPPCAPLAKGEPKSGGCYFPEDRLPIEDVKLKTCFVQNPA 480
           K KKLQWNCT+VEGYSSDVVP PCAPLAK    S GC FPED+LPIEDVKL TC  +N A
Sbjct: 421 KPKKLQWNCTNVEGYSSDVVPLPCAPLAKEVVSSDGCPFPEDQLPIEDVKLMTCITKNHA 480

Query: 481 S 482
           +
Sbjct: 481 T 481

BLAST of Tan0007429 vs. NCBI nr
Match: XP_022957618.1 (probable polygalacturonase [Cucurbita moschata])

HSP 1 Score: 891.7 bits (2303), Expect = 2.7e-255
Identity = 428/481 (88.98%), Postives = 452/481 (93.97%), Query Frame = 0

Query: 1   MGLPKKSTQTHVTAARISAVIVLALVSLATVDGKKPCPFSNYLEFPALSCRKHTAVLTQF 60
           M LP K T+T +TAARIS VI+LALVSL +VDGKK CPFS+YLEFPA+SCRKHTAVLT F
Sbjct: 1   MDLPHKPTKTQITAARISVVILLALVSLGSVDGKKSCPFSSYLEFPAISCRKHTAVLTDF 60

Query: 61  GGVGDGVTSNTHAFRQAVEHLSPLALEGGAQLIVPPGKWLTGSFNLTSHFTLFLHKDAQI 120
           GG+GDGVTSNT+AFR+A+EHLS LA++GGAQLIVPPGKWLTGSFNLTSHFTLF+HKDAQI
Sbjct: 61  GGIGDGVTSNTNAFREAMEHLSTLAVDGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAQI 120

Query: 121 LASQDESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWSK 180
           LASQDESEWPLVEVLPSYGRGRDAP GRFSSLIFGTNLTDVVITGNNGTIDGQGSYWW K
Sbjct: 121 LASQDESEWPLVEVLPSYGRGRDAPDGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWDK 180

Query: 181 FRKNELNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240
           F KNE NVTRPYMIEIMYS+QIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN
Sbjct: 181 FHKNEFNVTRPYMIEIMYSNQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240

Query: 241 TDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLLIRRLTCISPDSAG 300
           TDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGI+FGMPTEDLLIRRLTCISPDSAG
Sbjct: 241 TDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIQFGMPTEDLLIRRLTCISPDSAG 300

Query: 301 IALGSEMSGGIRNVRIENVTAINTQSAVRIKTARGRGGFVKDIFVRRMSLSTMKYVFWMT 360
           IALGSEMSGGIRNVRIENVTAINTQSAVRIKTA GRGG+VKDIFVR+M LSTMKYVFWMT
Sbjct: 301 IALGSEMSGGIRNVRIENVTAINTQSAVRIKTAIGRGGYVKDIFVRQMFLSTMKYVFWMT 360

Query: 361 GNYKSHPDEKFDPNALPEIKNINYRDVVAENVTYSARLEGISGDTFTDICISNVKIGLAA 420
           G+YKSHPDEKFDP ALPEIKNINYRDVVAENVTYSARL+GISGD FTDICISN+KIGL A
Sbjct: 361 GDYKSHPDEKFDPKALPEIKNINYRDVVAENVTYSARLDGISGDPFTDICISNIKIGLTA 420

Query: 421 KAKKLQWNCTDVEGYSSDVVPPPCAPLAKGEPKSGGCYFPEDRLPIEDVKLKTCFVQNPA 480
           K KKLQWNCT+VEGYSSDVVPPPCAPLAK    S GC FPEDRLPIED+KL TC  +N A
Sbjct: 421 KQKKLQWNCTNVEGYSSDVVPPPCAPLAKEVVSSDGCPFPEDRLPIEDLKLMTCVTKNHA 480

Query: 481 S 482
           +
Sbjct: 481 T 481

BLAST of Tan0007429 vs. NCBI nr
Match: XP_008437531.1 (PREDICTED: probable polygalacturonase [Cucumis melo] >KAA0042595.1 putative polygalacturonase [Cucumis melo var. makuwa] >TYK05998.1 putative polygalacturonase [Cucumis melo var. makuwa])

HSP 1 Score: 869.0 bits (2244), Expect = 1.9e-248
Identity = 421/479 (87.89%), Postives = 444/479 (92.69%), Query Frame = 0

Query: 1   MGLPKKSTQTHVTAARISAVIVLALVSLATVDGKKPCPFSNYLEFPALSCRKHTAVLTQF 60
           M LP+K T+TH  AARISAVI+LALVSLATV+GK+ CP +NYL+ PA++CR+HTAVLT F
Sbjct: 1   MDLPQKPTKTHDAAARISAVILLALVSLATVEGKRQCP-TNYLQVPAINCRQHTAVLTDF 60

Query: 61  GGVGDGVTSNTHAFRQAVEHLSPLALEGGAQLIVPPGKWLTGSFNLTSHFTLFLHKDAQI 120
           GGVGDGVTSNT AFR+A+EHLSPLA  GGAQLIVPPGKWLTGSFNLTSHFTLF+HKDA I
Sbjct: 61  GGVGDGVTSNTQAFRRAIEHLSPLAANGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDATI 120

Query: 121 LASQDESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWSK 180
           LASQDESEWP V +LPSYG GRDAPGGR+SSLI+GTNLTDVVITGNNGTIDGQGSYWW K
Sbjct: 121 LASQDESEWPQVAILPSYGVGRDAPGGRYSSLIYGTNLTDVVITGNNGTIDGQGSYWWDK 180

Query: 181 FRKNELNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240
           F K ELN+TRPYMIEI+YSDQIQISNLTLVNSPSWFVHPIYS+NVIIQGLTILAPIDSPN
Sbjct: 181 FHKGELNLTRPYMIEILYSDQIQISNLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSPN 240

Query: 241 TDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLLIRRLTCISPDSAG 300
           TDGIDPDSC N RIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDL+IRRLTCISPDSAG
Sbjct: 241 TDGIDPDSCSNIRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAG 300

Query: 301 IALGSEMSGGIRNVRIENVTAINTQSAVRIKTARGRGGFVKDIFVRRMSLSTMKYVFWMT 360
           IALGSEMSGGIRNVRIENVT INTQSAVRIKTARGRGGFVKDIFVR M LSTMKYVFWMT
Sbjct: 301 IALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMT 360

Query: 361 GNYKSHPDEKFDPNALPEIKNINYRDVVAENVTYSARLEGISGDTFTDICISNVKIGLAA 420
           GNYKSHPDEKFDP ALPEI NINYRDVVAENVTYSARLEGISGD FT+ICISNVKIGL A
Sbjct: 361 GNYKSHPDEKFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLTA 420

Query: 421 KAKKLQWNCTDVEGYSSDVVPPPCAPLAKGEPKSGGCYFPEDRLPIEDVKLKTCFVQNP 480
           K KKLQWNCTDVEG SSDVVPPPCAPLAK   KSGGC FP D+LPIE V+LKTCFVQNP
Sbjct: 421 KPKKLQWNCTDVEGISSDVVPPPCAPLAKA-AKSGGCDFPADQLPIEKVQLKTCFVQNP 477

BLAST of Tan0007429 vs. ExPASy TrEMBL
Match: A0A6J1K3G8 (probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111491343 PE=3 SV=1)

HSP 1 Score: 895.2 bits (2312), Expect = 1.2e-256
Identity = 431/481 (89.60%), Postives = 452/481 (93.97%), Query Frame = 0

Query: 1   MGLPKKSTQTHVTAARISAVIVLALVSLATVDGKKPCPFSNYLEFPALSCRKHTAVLTQF 60
           M LP K T+T +TA RISAVI+L LVSLA+VDGKKPCPFS+YLEF A+SCRKHTAVLT F
Sbjct: 1   MDLPHKPTKTQITAVRISAVILLVLVSLASVDGKKPCPFSSYLEFSAISCRKHTAVLTDF 60

Query: 61  GGVGDGVTSNTHAFRQAVEHLSPLALEGGAQLIVPPGKWLTGSFNLTSHFTLFLHKDAQI 120
           GGVGDGVTSNT+AFR+A+EHLS LA++GG+QLIVPPGKWLTGSFNLTSHFTLF+HKDAQI
Sbjct: 61  GGVGDGVTSNTNAFREAIEHLSTLAIDGGSQLIVPPGKWLTGSFNLTSHFTLFVHKDAQI 120

Query: 121 LASQDESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWSK 180
           LASQDESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWW K
Sbjct: 121 LASQDESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWDK 180

Query: 181 FRKNELNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240
           F KNE NVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN
Sbjct: 181 FHKNEFNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240

Query: 241 TDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLLIRRLTCISPDSAG 300
           TDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGI+FGMPTEDLLIRRLTCISPDSAG
Sbjct: 241 TDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIQFGMPTEDLLIRRLTCISPDSAG 300

Query: 301 IALGSEMSGGIRNVRIENVTAINTQSAVRIKTARGRGGFVKDIFVRRMSLSTMKYVFWMT 360
           IALGSEMSGGIRNVRIENVTAINTQSAVRIKTA GRGG+VKDIFVR+M LSTMKYVFWMT
Sbjct: 301 IALGSEMSGGIRNVRIENVTAINTQSAVRIKTAIGRGGYVKDIFVRQMFLSTMKYVFWMT 360

Query: 361 GNYKSHPDEKFDPNALPEIKNINYRDVVAENVTYSARLEGISGDTFTDICISNVKIGLAA 420
           GNYKSHPDEKFDP ALPEIKNINYRDVVAENVTYSARL+GISGD FTDICISNVKIGL A
Sbjct: 361 GNYKSHPDEKFDPKALPEIKNINYRDVVAENVTYSARLDGISGDPFTDICISNVKIGLTA 420

Query: 421 KAKKLQWNCTDVEGYSSDVVPPPCAPLAKGEPKSGGCYFPEDRLPIEDVKLKTCFVQNPA 480
           K KKLQWNCT+VEGYSSDVVP PCAPLAK    S GC FPED+LPIEDVKL TC  +N A
Sbjct: 421 KPKKLQWNCTNVEGYSSDVVPLPCAPLAKEVVSSDGCPFPEDQLPIEDVKLMTCITKNHA 480

Query: 481 S 482
           +
Sbjct: 481 T 481

BLAST of Tan0007429 vs. ExPASy TrEMBL
Match: A0A6J1H0R5 (probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111459096 PE=3 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.3e-255
Identity = 428/481 (88.98%), Postives = 452/481 (93.97%), Query Frame = 0

Query: 1   MGLPKKSTQTHVTAARISAVIVLALVSLATVDGKKPCPFSNYLEFPALSCRKHTAVLTQF 60
           M LP K T+T +TAARIS VI+LALVSL +VDGKK CPFS+YLEFPA+SCRKHTAVLT F
Sbjct: 1   MDLPHKPTKTQITAARISVVILLALVSLGSVDGKKSCPFSSYLEFPAISCRKHTAVLTDF 60

Query: 61  GGVGDGVTSNTHAFRQAVEHLSPLALEGGAQLIVPPGKWLTGSFNLTSHFTLFLHKDAQI 120
           GG+GDGVTSNT+AFR+A+EHLS LA++GGAQLIVPPGKWLTGSFNLTSHFTLF+HKDAQI
Sbjct: 61  GGIGDGVTSNTNAFREAMEHLSTLAVDGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDAQI 120

Query: 121 LASQDESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWSK 180
           LASQDESEWPLVEVLPSYGRGRDAP GRFSSLIFGTNLTDVVITGNNGTIDGQGSYWW K
Sbjct: 121 LASQDESEWPLVEVLPSYGRGRDAPDGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWDK 180

Query: 181 FRKNELNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240
           F KNE NVTRPYMIEIMYS+QIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN
Sbjct: 181 FHKNEFNVTRPYMIEIMYSNQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240

Query: 241 TDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLLIRRLTCISPDSAG 300
           TDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGI+FGMPTEDLLIRRLTCISPDSAG
Sbjct: 241 TDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIQFGMPTEDLLIRRLTCISPDSAG 300

Query: 301 IALGSEMSGGIRNVRIENVTAINTQSAVRIKTARGRGGFVKDIFVRRMSLSTMKYVFWMT 360
           IALGSEMSGGIRNVRIENVTAINTQSAVRIKTA GRGG+VKDIFVR+M LSTMKYVFWMT
Sbjct: 301 IALGSEMSGGIRNVRIENVTAINTQSAVRIKTAIGRGGYVKDIFVRQMFLSTMKYVFWMT 360

Query: 361 GNYKSHPDEKFDPNALPEIKNINYRDVVAENVTYSARLEGISGDTFTDICISNVKIGLAA 420
           G+YKSHPDEKFDP ALPEIKNINYRDVVAENVTYSARL+GISGD FTDICISN+KIGL A
Sbjct: 361 GDYKSHPDEKFDPKALPEIKNINYRDVVAENVTYSARLDGISGDPFTDICISNIKIGLTA 420

Query: 421 KAKKLQWNCTDVEGYSSDVVPPPCAPLAKGEPKSGGCYFPEDRLPIEDVKLKTCFVQNPA 480
           K KKLQWNCT+VEGYSSDVVPPPCAPLAK    S GC FPEDRLPIED+KL TC  +N A
Sbjct: 421 KQKKLQWNCTNVEGYSSDVVPPPCAPLAKEVVSSDGCPFPEDRLPIEDLKLMTCVTKNHA 480

Query: 481 S 482
           +
Sbjct: 481 T 481

BLAST of Tan0007429 vs. ExPASy TrEMBL
Match: A0A5D3C461 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold376G00950 PE=3 SV=1)

HSP 1 Score: 869.0 bits (2244), Expect = 9.2e-249
Identity = 421/479 (87.89%), Postives = 444/479 (92.69%), Query Frame = 0

Query: 1   MGLPKKSTQTHVTAARISAVIVLALVSLATVDGKKPCPFSNYLEFPALSCRKHTAVLTQF 60
           M LP+K T+TH  AARISAVI+LALVSLATV+GK+ CP +NYL+ PA++CR+HTAVLT F
Sbjct: 1   MDLPQKPTKTHDAAARISAVILLALVSLATVEGKRQCP-TNYLQVPAINCRQHTAVLTDF 60

Query: 61  GGVGDGVTSNTHAFRQAVEHLSPLALEGGAQLIVPPGKWLTGSFNLTSHFTLFLHKDAQI 120
           GGVGDGVTSNT AFR+A+EHLSPLA  GGAQLIVPPGKWLTGSFNLTSHFTLF+HKDA I
Sbjct: 61  GGVGDGVTSNTQAFRRAIEHLSPLAANGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDATI 120

Query: 121 LASQDESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWSK 180
           LASQDESEWP V +LPSYG GRDAPGGR+SSLI+GTNLTDVVITGNNGTIDGQGSYWW K
Sbjct: 121 LASQDESEWPQVAILPSYGVGRDAPGGRYSSLIYGTNLTDVVITGNNGTIDGQGSYWWDK 180

Query: 181 FRKNELNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240
           F K ELN+TRPYMIEI+YSDQIQISNLTLVNSPSWFVHPIYS+NVIIQGLTILAPIDSPN
Sbjct: 181 FHKGELNLTRPYMIEILYSDQIQISNLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSPN 240

Query: 241 TDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLLIRRLTCISPDSAG 300
           TDGIDPDSC N RIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDL+IRRLTCISPDSAG
Sbjct: 241 TDGIDPDSCSNIRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAG 300

Query: 301 IALGSEMSGGIRNVRIENVTAINTQSAVRIKTARGRGGFVKDIFVRRMSLSTMKYVFWMT 360
           IALGSEMSGGIRNVRIENVT INTQSAVRIKTARGRGGFVKDIFVR M LSTMKYVFWMT
Sbjct: 301 IALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMT 360

Query: 361 GNYKSHPDEKFDPNALPEIKNINYRDVVAENVTYSARLEGISGDTFTDICISNVKIGLAA 420
           GNYKSHPDEKFDP ALPEI NINYRDVVAENVTYSARLEGISGD FT+ICISNVKIGL A
Sbjct: 361 GNYKSHPDEKFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLTA 420

Query: 421 KAKKLQWNCTDVEGYSSDVVPPPCAPLAKGEPKSGGCYFPEDRLPIEDVKLKTCFVQNP 480
           K KKLQWNCTDVEG SSDVVPPPCAPLAK   KSGGC FP D+LPIE V+LKTCFVQNP
Sbjct: 421 KPKKLQWNCTDVEGISSDVVPPPCAPLAKA-AKSGGCDFPADQLPIEKVQLKTCFVQNP 477

BLAST of Tan0007429 vs. ExPASy TrEMBL
Match: A0A1S3AUV5 (probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103482916 PE=3 SV=1)

HSP 1 Score: 869.0 bits (2244), Expect = 9.2e-249
Identity = 421/479 (87.89%), Postives = 444/479 (92.69%), Query Frame = 0

Query: 1   MGLPKKSTQTHVTAARISAVIVLALVSLATVDGKKPCPFSNYLEFPALSCRKHTAVLTQF 60
           M LP+K T+TH  AARISAVI+LALVSLATV+GK+ CP +NYL+ PA++CR+HTAVLT F
Sbjct: 1   MDLPQKPTKTHDAAARISAVILLALVSLATVEGKRQCP-TNYLQVPAINCRQHTAVLTDF 60

Query: 61  GGVGDGVTSNTHAFRQAVEHLSPLALEGGAQLIVPPGKWLTGSFNLTSHFTLFLHKDAQI 120
           GGVGDGVTSNT AFR+A+EHLSPLA  GGAQLIVPPGKWLTGSFNLTSHFTLF+HKDA I
Sbjct: 61  GGVGDGVTSNTQAFRRAIEHLSPLAANGGAQLIVPPGKWLTGSFNLTSHFTLFVHKDATI 120

Query: 121 LASQDESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWSK 180
           LASQDESEWP V +LPSYG GRDAPGGR+SSLI+GTNLTDVVITGNNGTIDGQGSYWW K
Sbjct: 121 LASQDESEWPQVAILPSYGVGRDAPGGRYSSLIYGTNLTDVVITGNNGTIDGQGSYWWDK 180

Query: 181 FRKNELNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPN 240
           F K ELN+TRPYMIEI+YSDQIQISNLTLVNSPSWFVHPIYS+NVIIQGLTILAPIDSPN
Sbjct: 181 FHKGELNLTRPYMIEILYSDQIQISNLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDSPN 240

Query: 241 TDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLLIRRLTCISPDSAG 300
           TDGIDPDSC N RIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDL+IRRLTCISPDSAG
Sbjct: 241 TDGIDPDSCSNIRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIRRLTCISPDSAG 300

Query: 301 IALGSEMSGGIRNVRIENVTAINTQSAVRIKTARGRGGFVKDIFVRRMSLSTMKYVFWMT 360
           IALGSEMSGGIRNVRIENVT INTQSAVRIKTARGRGGFVKDIFVR M LSTMKYVFWMT
Sbjct: 301 IALGSEMSGGIRNVRIENVTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFWMT 360

Query: 361 GNYKSHPDEKFDPNALPEIKNINYRDVVAENVTYSARLEGISGDTFTDICISNVKIGLAA 420
           GNYKSHPDEKFDP ALPEI NINYRDVVAENVTYSARLEGISGD FT+ICISNVKIGL A
Sbjct: 361 GNYKSHPDEKFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGLTA 420

Query: 421 KAKKLQWNCTDVEGYSSDVVPPPCAPLAKGEPKSGGCYFPEDRLPIEDVKLKTCFVQNP 480
           K KKLQWNCTDVEG SSDVVPPPCAPLAK   KSGGC FP D+LPIE V+LKTCFVQNP
Sbjct: 421 KPKKLQWNCTDVEGISSDVVPPPCAPLAKA-AKSGGCDFPADQLPIEKVQLKTCFVQNP 477

BLAST of Tan0007429 vs. ExPASy TrEMBL
Match: A0A0A0KNF9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G140480 PE=3 SV=1)

HSP 1 Score: 849.4 bits (2193), Expect = 7.6e-243
Identity = 411/481 (85.45%), Postives = 443/481 (92.10%), Query Frame = 0

Query: 1   MGLPKKSTQTHVTAARISAVIVLALVSLATVDGKKPCPFSNYLEFPALSCRKHTAVLTQF 60
           M LP+  T+TH  AARISAVI+LAL+SLATV+GK+ CP +NYL+ PA+SCRKHTA++T F
Sbjct: 1   MYLPQDPTKTHDAAARISAVILLALLSLATVEGKRSCP-TNYLQVPAISCRKHTALITDF 60

Query: 61  GGVGDGVTSNTHAFRQAVEHLSPLALEGGAQLIVPPGKWLTGSFNLTSHFTLFLHKDAQI 120
           GGVGDGVTSNT AFR+A+EHLS LA +GGAQL VPPGKWLTGSFNLTSHFTLF+HKDA+I
Sbjct: 61  GGVGDGVTSNTQAFRRAIEHLSSLAADGGAQLTVPPGKWLTGSFNLTSHFTLFVHKDAKI 120

Query: 121 LASQ--DESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWW 180
           L SQ  +ESEWP V +LPSYG GRDAPGGR+SSLIFGTNLTDVVITGNNGTIDGQG+YWW
Sbjct: 121 LGSQVENESEWPQVALLPSYGVGRDAPGGRYSSLIFGTNLTDVVITGNNGTIDGQGAYWW 180

Query: 181 SKFRKNELNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDS 240
            KF K ELN+TRPY+IEIMYSDQIQIS+LTLVNSPSWFVHPIYS+NVIIQGLTILAPIDS
Sbjct: 181 DKFHKGELNLTRPYLIEIMYSDQIQISDLTLVNSPSWFVHPIYSKNVIIQGLTILAPIDS 240

Query: 241 PNTDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLLIRRLTCISPDS 300
           PNTDGIDPDSC NTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDL+I+RLTCISPDS
Sbjct: 241 PNTDGIDPDSCSNTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPTEDLVIKRLTCISPDS 300

Query: 301 AGIALGSEMSGGIRNVRIENVTAINTQSAVRIKTARGRGGFVKDIFVRRMSLSTMKYVFW 360
           AG+ALGSEMSGGIRNVRIEN T INTQSAVRIKTARGRGGFVKDIFVR M LSTMKYVFW
Sbjct: 301 AGVALGSEMSGGIRNVRIENFTGINTQSAVRIKTARGRGGFVKDIFVRGMYLSTMKYVFW 360

Query: 361 MTGNYKSHPDEKFDPNALPEIKNINYRDVVAENVTYSARLEGISGDTFTDICISNVKIGL 420
           MTGNYKSHPD+KFDP ALPEI NINYRDVVAENVTYSARLEGISGD FT+ICISNVKIGL
Sbjct: 361 MTGNYKSHPDDKFDPAALPEITNINYRDVVAENVTYSARLEGISGDPFTNICISNVKIGL 420

Query: 421 AAKAKKLQWNCTDVEGYSSDVVPPPCAPLAKGEPKSGGCYFPEDRLPIEDVKLKTCFVQN 480
            A  KKLQWNCTDVEG+SSDVVPPPCAPLAK   KSGGC FPED+LPIE V+LKTCFVQN
Sbjct: 421 TATPKKLQWNCTDVEGFSSDVVPPPCAPLAKA-GKSGGCDFPEDQLPIERVQLKTCFVQN 479

BLAST of Tan0007429 vs. TAIR 10
Match: AT3G48950.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 660.6 bits (1703), Expect = 9.6e-190
Identity = 306/437 (70.02%), Postives = 362/437 (82.84%), Query Frame = 0

Query: 43  LEFPALSCRKHTAVLTQFGGVGDGVTSNTHAFRQAVEHLSPLALEGGAQLIVPPGKWLTG 102
           +EF AL+CRKH+A+LT FG VGDG TSNT AFR A+  LS +A +GGAQL+VPPGKWLTG
Sbjct: 33  IEFSALNCRKHSAILTDFGAVGDGKTSNTKAFRNAISKLSQMATDGGAQLVVPPGKWLTG 92

Query: 103 SFNLTSHFTLFLHKDAQILASQDESEWPLVEVLPSYGRGRDAPG-GRFSSLIFGTNLTDV 162
           SFNLTSHFTLF+ + A ILASQDESEWP++  LPSYG+GRD  G GRF+SLI GTNLTDV
Sbjct: 93  SFNLTSHFTLFIQRGATILASQDESEWPVIAPLPSYGKGRDGTGTGRFNSLISGTNLTDV 152

Query: 163 VITGNNGTIDGQGSYWWSKFRKNELNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIY 222
           VITGNNGTI+GQG YWW KF+K +  +TRPY+IEI++S  IQISN+TL++SPSW +HP+Y
Sbjct: 153 VITGNNGTINGQGQYWWDKFKKKQFKITRPYLIEILFSKNIQISNITLIDSPSWNIHPVY 212

Query: 223 SRNVIIQGLTILAPIDSPNTDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIKFGM 282
             +VI++ +T+LAP+  PNTDGI+PDSC NT IEDC+IVSGDDCIAVKSGWDQYGIKFGM
Sbjct: 213 CNSVIVKSVTVLAPVTVPNTDGINPDSCTNTLIEDCYIVSGDDCIAVKSGWDQYGIKFGM 272

Query: 283 PTEDLLIRRLTCISPDSAGIALGSEMSGGIRNVRIENVTAINTQSAVRIKTARGRGGFVK 342
           PT+ L IRRLTCISP SAG+ALGSEMSGGI++VRIE+VT  NT+SA+RIKTA GRG +VK
Sbjct: 273 PTQQLSIRRLTCISPKSAGVALGSEMSGGIKDVRIEDVTLTNTESAIRIKTAVGRGAYVK 332

Query: 343 DIFVRRMSLSTMKYVFWMTGNYKSHPDEKFDPNALPEIKNINYRDVVAENVTYSARLEGI 402
           DI+ RR+++ TMKYVFWM+GNY SHPDE FDP ALPEI NINYRD+ AENVT SA L+GI
Sbjct: 333 DIYARRITMKTMKYVFWMSGNYGSHPDEGFDPKALPEITNINYRDMTAENVTMSASLDGI 392

Query: 403 SGDTFTDICISNVKIGLAAKAKKLQWNCTDVEGYSSDVVPPPCA--PLAKGEPKSGGCYF 462
             D FT ICISNV I LAAKAKK+QWNCTDV G +S V P PC+  P  K + K+  C F
Sbjct: 393 DKDPFTGICISNVTIALAAKAKKMQWNCTDVAGVTSRVTPEPCSLLPEKKAQAKNVDCAF 452

Query: 463 PEDRLPIEDVKLKTCFV 477
           P D +PIE V LK CF+
Sbjct: 453 PSDLIPIESVVLKKCFL 469

BLAST of Tan0007429 vs. TAIR 10
Match: AT3G61490.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 639.4 bits (1648), Expect = 2.3e-183
Identity = 302/466 (64.81%), Postives = 371/466 (79.61%), Query Frame = 0

Query: 20  VIVLALVSLATV-DGKKPCPFSNYLEFPALSCRKHTAVLTQFGGVGDGVTSNTHAFRQAV 79
           V ++ L +L T+  G++        E+ A+ CR H+A +T++GGVGDG T NT AF+ AV
Sbjct: 9   VTIITLSNLITISQGRRVSQSFETFEYTAIICRSHSASITEYGGVGDGKTLNTKAFQSAV 68

Query: 80  EHLSPLALEGGAQLIVPPGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVEVLPSY 139
           +HLS  + EGGAQL VP GKWLTGSFNLTSHFTLFLHKDA +LA+QD +E+P+++ LPSY
Sbjct: 69  DHLSQYSSEGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAILLAAQDLNEYPILKALPSY 128

Query: 140 GRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWSKFRKNELNVTRPYMIEIMY 199
           GRGRDA GGRF+SLIFGTNL+DV+ITGNNGTIDGQGS+WW KF   +L  TRPY+IE+M+
Sbjct: 129 GRGRDAAGGRFASLIFGTNLSDVIITGNNGTIDGQGSFWWQKFHGGKLKYTRPYLIELMF 188

Query: 200 SDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPNTDGIDPDSCINTRIEDCF 259
           SD IQISNLT ++SPSW +HP+YS N+I++G+TI+AP+ SPNTDGI+PDSC NTRIEDC+
Sbjct: 189 SDTIQISNLTFLDSPSWNIHPVYSSNIIVKGVTIIAPVKSPNTDGINPDSCTNTRIEDCY 248

Query: 260 IVSGDDCIAVKSGWDQYGIKFGMPTEDLLIRRLTCISPDSAGIALGSEMSGGIRNVRIEN 319
           I+SGDDCIAVKSGWD+YGI FGMPT+ L+IRRLTCISP SA IALGSEMSGGI +VR E+
Sbjct: 249 IISGDDCIAVKSGWDEYGISFGMPTKHLVIRRLTCISPYSAAIALGSEMSGGIEDVRAED 308

Query: 320 VTAINTQSAVRIKTARGRGGFVKDIFVRRMSLSTMKYVFWMTGNYKSHPDEKFDPNALPE 379
           +TA  T+S VRIKTA GRG FVK+I+V+ M+L TMK+VFWMTGNYK+H D  +DP+ALPE
Sbjct: 309 ITAYQTESGVRIKTAVGRGAFVKNIYVKGMNLHTMKWVFWMTGNYKAHADSHYDPHALPE 368

Query: 380 IKNINYRDVVAENVTYSARLEGISGDTFTDICISNVKIGLAAKAKKLQWNCTDVEGYSSD 439
           I  INYRD+VAENV+ + RLEGISGD FT ICISN  I +AAK KK  W C+DVEG +S 
Sbjct: 369 ITGINYRDIVAENVSMAGRLEGISGDPFTGICISNATISMAAKHKKAIWMCSDVEGVTSG 428

Query: 440 VVPPPCAPLAKGEPKS-------GGCYFPEDRLPIEDVKLKTCFVQ 478
           V P PC  L   E ++       GGC FP D L I++V+LKTC  Q
Sbjct: 429 VDPKPCDLLDGQESETTKKKMIDGGCDFPTDVLEIDNVELKTCSYQ 474

BLAST of Tan0007429 vs. TAIR 10
Match: AT3G61490.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 639.4 bits (1648), Expect = 2.3e-183
Identity = 302/466 (64.81%), Postives = 371/466 (79.61%), Query Frame = 0

Query: 20  VIVLALVSLATV-DGKKPCPFSNYLEFPALSCRKHTAVLTQFGGVGDGVTSNTHAFRQAV 79
           V ++ L +L T+  G++        E+ A+ CR H+A +T++GGVGDG T NT AF+ AV
Sbjct: 9   VTIITLSNLITISQGRRVSQSFETFEYTAIICRSHSASITEYGGVGDGKTLNTKAFQSAV 68

Query: 80  EHLSPLALEGGAQLIVPPGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVEVLPSY 139
           +HLS  + EGGAQL VP GKWLTGSFNLTSHFTLFLHKDA +LA+QD +E+P+++ LPSY
Sbjct: 69  DHLSQYSSEGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAILLAAQDLNEYPILKALPSY 128

Query: 140 GRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWSKFRKNELNVTRPYMIEIMY 199
           GRGRDA GGRF+SLIFGTNL+DV+ITGNNGTIDGQGS+WW KF   +L  TRPY+IE+M+
Sbjct: 129 GRGRDAAGGRFASLIFGTNLSDVIITGNNGTIDGQGSFWWQKFHGGKLKYTRPYLIELMF 188

Query: 200 SDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPNTDGIDPDSCINTRIEDCF 259
           SD IQISNLT ++SPSW +HP+YS N+I++G+TI+AP+ SPNTDGI+PDSC NTRIEDC+
Sbjct: 189 SDTIQISNLTFLDSPSWNIHPVYSSNIIVKGVTIIAPVKSPNTDGINPDSCTNTRIEDCY 248

Query: 260 IVSGDDCIAVKSGWDQYGIKFGMPTEDLLIRRLTCISPDSAGIALGSEMSGGIRNVRIEN 319
           I+SGDDCIAVKSGWD+YGI FGMPT+ L+IRRLTCISP SA IALGSEMSGGI +VR E+
Sbjct: 249 IISGDDCIAVKSGWDEYGISFGMPTKHLVIRRLTCISPYSAAIALGSEMSGGIEDVRAED 308

Query: 320 VTAINTQSAVRIKTARGRGGFVKDIFVRRMSLSTMKYVFWMTGNYKSHPDEKFDPNALPE 379
           +TA  T+S VRIKTA GRG FVK+I+V+ M+L TMK+VFWMTGNYK+H D  +DP+ALPE
Sbjct: 309 ITAYQTESGVRIKTAVGRGAFVKNIYVKGMNLHTMKWVFWMTGNYKAHADSHYDPHALPE 368

Query: 380 IKNINYRDVVAENVTYSARLEGISGDTFTDICISNVKIGLAAKAKKLQWNCTDVEGYSSD 439
           I  INYRD+VAENV+ + RLEGISGD FT ICISN  I +AAK KK  W C+DVEG +S 
Sbjct: 369 ITGINYRDIVAENVSMAGRLEGISGDPFTGICISNATISMAAKHKKAIWMCSDVEGVTSG 428

Query: 440 VVPPPCAPLAKGEPKS-------GGCYFPEDRLPIEDVKLKTCFVQ 478
           V P PC  L   E ++       GGC FP D L I++V+LKTC  Q
Sbjct: 429 VDPKPCDLLDGQESETTKKKMIDGGCDFPTDVLEIDNVELKTCSYQ 474

BLAST of Tan0007429 vs. TAIR 10
Match: AT3G61490.3 (Pectin lyase-like superfamily protein )

HSP 1 Score: 639.4 bits (1648), Expect = 2.3e-183
Identity = 302/466 (64.81%), Postives = 371/466 (79.61%), Query Frame = 0

Query: 20  VIVLALVSLATV-DGKKPCPFSNYLEFPALSCRKHTAVLTQFGGVGDGVTSNTHAFRQAV 79
           V ++ L +L T+  G++        E+ A+ CR H+A +T++GGVGDG T NT AF+ AV
Sbjct: 9   VTIITLSNLITISQGRRVSQSFETFEYTAIICRSHSASITEYGGVGDGKTLNTKAFQSAV 68

Query: 80  EHLSPLALEGGAQLIVPPGKWLTGSFNLTSHFTLFLHKDAQILASQDESEWPLVEVLPSY 139
           +HLS  + EGGAQL VP GKWLTGSFNLTSHFTLFLHKDA +LA+QD +E+P+++ LPSY
Sbjct: 69  DHLSQYSSEGGAQLFVPAGKWLTGSFNLTSHFTLFLHKDAILLAAQDLNEYPILKALPSY 128

Query: 140 GRGRDAPGGRFSSLIFGTNLTDVVITGNNGTIDGQGSYWWSKFRKNELNVTRPYMIEIMY 199
           GRGRDA GGRF+SLIFGTNL+DV+ITGNNGTIDGQGS+WW KF   +L  TRPY+IE+M+
Sbjct: 129 GRGRDAAGGRFASLIFGTNLSDVIITGNNGTIDGQGSFWWQKFHGGKLKYTRPYLIELMF 188

Query: 200 SDQIQISNLTLVNSPSWFVHPIYSRNVIIQGLTILAPIDSPNTDGIDPDSCINTRIEDCF 259
           SD IQISNLT ++SPSW +HP+YS N+I++G+TI+AP+ SPNTDGI+PDSC NTRIEDC+
Sbjct: 189 SDTIQISNLTFLDSPSWNIHPVYSSNIIVKGVTIIAPVKSPNTDGINPDSCTNTRIEDCY 248

Query: 260 IVSGDDCIAVKSGWDQYGIKFGMPTEDLLIRRLTCISPDSAGIALGSEMSGGIRNVRIEN 319
           I+SGDDCIAVKSGWD+YGI FGMPT+ L+IRRLTCISP SA IALGSEMSGGI +VR E+
Sbjct: 249 IISGDDCIAVKSGWDEYGISFGMPTKHLVIRRLTCISPYSAAIALGSEMSGGIEDVRAED 308

Query: 320 VTAINTQSAVRIKTARGRGGFVKDIFVRRMSLSTMKYVFWMTGNYKSHPDEKFDPNALPE 379
           +TA  T+S VRIKTA GRG FVK+I+V+ M+L TMK+VFWMTGNYK+H D  +DP+ALPE
Sbjct: 309 ITAYQTESGVRIKTAVGRGAFVKNIYVKGMNLHTMKWVFWMTGNYKAHADSHYDPHALPE 368

Query: 380 IKNINYRDVVAENVTYSARLEGISGDTFTDICISNVKIGLAAKAKKLQWNCTDVEGYSSD 439
           I  INYRD+VAENV+ + RLEGISGD FT ICISN  I +AAK KK  W C+DVEG +S 
Sbjct: 369 ITGINYRDIVAENVSMAGRLEGISGDPFTGICISNATISMAAKHKKAIWMCSDVEGVTSG 428

Query: 440 VVPPPCAPLAKGEPKS-------GGCYFPEDRLPIEDVKLKTCFVQ 478
           V P PC  L   E ++       GGC FP D L I++V+LKTC  Q
Sbjct: 429 VDPKPCDLLDGQESETTKKKMIDGGCDFPTDVLEIDNVELKTCSYQ 474

BLAST of Tan0007429 vs. TAIR 10
Match: AT4G23500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 639.4 bits (1648), Expect = 2.3e-183
Identity = 299/433 (69.05%), Postives = 357/433 (82.45%), Query Frame = 0

Query: 44  EFPALSCRKHTAVLTQFGGVGDGVTSNTHAFRQAVEHLSPLALEGGAQLIVPPGKWLTGS 103
           E+ A+SCR ++A L +FG VGDGVTSNT AFR AV  LS  A  GG+ L VP G+WLTG+
Sbjct: 57  EYSAISCRAYSASLDEFGAVGDGVTSNTAAFRDAVSQLSRFADYGGSLLFVPAGRWLTGN 116

Query: 104 FNLTSHFTLFLHKDAQILASQDESEWPLVEVLPSYGRGRDAPGGRFSSLIFGTNLTDVVI 163
           FNLTSHFTLFLH+DA ILASQ+ES++ ++E LPSYGRGRD  GGRF SL+FG+NLTDVVI
Sbjct: 117 FNLTSHFTLFLHRDAVILASQEESDYEVIEPLPSYGRGRDTDGGRFISLLFGSNLTDVVI 176

Query: 164 TGNNGTIDGQGSYWWSKFRKNELNVTRPYMIEIMYSDQIQISNLTLVNSPSWFVHPIYSR 223
           TG NGTIDGQG  WW KF++ EL  TRPY+IEIM+SD IQISNLT +NSPSW +HP+YS 
Sbjct: 177 TGENGTIDGQGEPWWGKFKRGELKYTRPYLIEIMHSDGIQISNLTFLNSPSWHIHPVYSS 236

Query: 224 NVIIQGLTILAPIDSPNTDGIDPDSCINTRIEDCFIVSGDDCIAVKSGWDQYGIKFGMPT 283
           N+ IQGLTILAP+  PNTDGI+PDSC NTRIEDC+IVSGDDCIAVKSGWDQYGI +GMPT
Sbjct: 237 NIYIQGLTILAPVTVPNTDGINPDSCTNTRIEDCYIVSGDDCIAVKSGWDQYGINYGMPT 296

Query: 284 EDLLIRRLTCISPDSAGIALGSEMSGGIRNVRIENVTAINTQSAVRIKTARGRGGFVKDI 343
           + LLIRRLTCISPDSA IALGSEMSGGI +VR E++ AIN++S +RIKTA GRGG+VKD+
Sbjct: 297 KQLLIRRLTCISPDSAVIALGSEMSGGIEDVRAEDIVAINSESGIRIKTAIGRGGYVKDV 356

Query: 344 FVRRMSLSTMKYVFWMTGNYKSHPDEKFDPNALPEIKNINYRDVVAENVTYSARLEGISG 403
           +VR M++ TMKYVFWMTG+Y SHPD+ +DP ALP I+NINY+D+VAENVT  A+L GISG
Sbjct: 357 YVRGMTMMTMKYVFWMTGSYGSHPDDHYDPKALPVIQNINYQDMVAENVTMPAQLAGISG 416

Query: 404 DTFTDICISNVKIGLAAKAKKLQWNCTDVEGYSSDVVPPPCAPLAKGEPKS-GGCYFPED 463
           D FT ICISNV I L+ K KK+ WNCTDV GY+S V P PC  L + +P +   C FPED
Sbjct: 417 DQFTGICISNVTITLSKKPKKVLWNCTDVSGYTSGVTPQPCQLLPEKQPGTVVPCNFPED 476

Query: 464 RLPIEDVKLKTCF 476
            +PI++VKL+ C+
Sbjct: 477 PIPIDEVKLQRCY 489

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
A7PZL31.4e-18068.36Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 ... [more]
Q9LW078.9e-3128.74Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572... [more]
Q7M1E74.1e-2827.74Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
Q9FY194.5e-2727.63Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1[more]
P276445.2e-2332.13Polygalacturonase OS=Rhizobium radiobacter OX=358 GN=pgl PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038906749.14.1e-25990.44probable polygalacturonase [Benincasa hispida][more]
XP_023532553.11.2e-25890.42probable polygalacturonase [Cucurbita pepo subsp. pepo][more]
XP_022996001.12.5e-25689.60probable polygalacturonase [Cucurbita maxima][more]
XP_022957618.12.7e-25588.98probable polygalacturonase [Cucurbita moschata][more]
XP_008437531.11.9e-24887.89PREDICTED: probable polygalacturonase [Cucumis melo] >KAA0042595.1 putative poly... [more]
Match NameE-valueIdentityDescription
A0A6J1K3G81.2e-25689.60probable polygalacturonase OS=Cucurbita maxima OX=3661 GN=LOC111491343 PE=3 SV=1[more]
A0A6J1H0R51.3e-25588.98probable polygalacturonase OS=Cucurbita moschata OX=3662 GN=LOC111459096 PE=3 SV... [more]
A0A5D3C4619.2e-24987.89Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A1S3AUV59.2e-24987.89probable polygalacturonase OS=Cucumis melo OX=3656 GN=LOC103482916 PE=3 SV=1[more]
A0A0A0KNF97.6e-24385.45Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G140480 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G48950.19.6e-19070.02Pectin lyase-like superfamily protein [more]
AT3G61490.12.3e-18364.81Pectin lyase-like superfamily protein [more]
AT3G61490.22.3e-18364.81Pectin lyase-like superfamily protein [more]
AT3G61490.32.3e-18364.81Pectin lyase-like superfamily protein [more]
AT4G23500.12.3e-18369.05Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 311..332
e-value: 200.0
score: 10.7
coord: 283..305
e-value: 2400.0
score: 4.5
coord: 222..248
e-value: 1100.0
score: 6.9
coord: 249..270
e-value: 600.0
score: 8.8
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 152..433
e-value: 1.6E-39
score: 135.9
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 44..439
e-value: 2.2E-112
score: 378.1
NoneNo IPR availablePANTHERPTHR31339PECTIN LYASE-RELATEDcoord: 18..475
NoneNo IPR availablePANTHERPTHR31339:SF12ENDO-POLYGALACTURONASE-LIKE PROTEINcoord: 18..475
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 48..450

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0007429.1Tan0007429.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity