Tan0007352 (gene) Snake gourd v1

Overview
NameTan0007352
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionPectinesterase
LocationLG06: 13886207 .. 13892988 (-)
RNA-Seq ExpressionTan0007352
SyntenyTan0007352
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACCAACCTATCTGCCTTTCTTCACAACTCTTCTCATTTGCGAGCTATGGCAAACACTAAAGTTTCATTGAAGAAAAAGAATGAGCTTCTTGTCTTAGCTCTCTTTGCTACTTTACTCACAAATGCTATTGTTGTCATTTCTGAATTCAATTATGATGAGAATAGTCTTAACAATGTGGGTATTGTAGAACACTCATGTGCCACCACTCTTTATCCACAACTATGTCATTCAACAATATCCTCCATCGTGGGAGCATCCGAGTTTTTGAGTGTAAAAGATATTGTTGAAATCTCGTTGAGTGTTGCTATAGATGCAGCTAAGTATAACCATGAAAACATCAAGAAACTTTCGATGTCGCTCAATGATCTTACTAAACGAGAGAGAATCACTCTCCGTGATTGTATCGTGACCACCGACAAGACGCTCGAAGACCTTGACAAGGTCGTAGAGGATCTTCGTGAGTATCCAAATAAGAAATCTATTATCCTATATGCTGATGACCTCAAAACGTTTCTCAGTTCTGCCATGACCAACCAGGTACTATTACTTATGAGGTCATTTTGGGATGACTTTTGAAATTTGAAAGAAAAGTTTTTAAAAGAAAGCACTTGTGTATAAGTATTTTATTGTGTTTGATATGCCTTTTTATATGTTTATGGATGACTAAAAATGCTTAATATCTTCTGTTACAGATTTATATATAATAATTAATGATCTGTTACTTATTTAAAACCAAATTATAAATTTGCAGGAGACTTGTTTGGATGGATTCTCAGACGAGGAAGCTGAAAAAAGGGTGTTGAAATTGATTGAAACCCCACATATTCACGTGACAAAGTTGTGTAGTAATGTTCTAGCCCTGGTCAAGAATTTGACTGACACTGATTTAACTTTAAGGGGCTTGGTCCCTGAGGACCCTAATAAGATAGCTTCATCCTGGCATGCTTACGAATCCGACCCCAAAATTGTACTCTTTTCAGACCTTACTAACGAGGTACTCATTTTTTTTAACTGGGTTAAAATAAAAAGTTCAAATTCAGTTGATGTAATTTCAATAAAACTTAAAATTAGTTTTGTTGTTATTTTAAAGTTAACTTTTTTAAAAATTAATTAAAAAGTAACCATAATTTTCATGCAAGTGAACCTATTTTAAAATTTTATAAAAGAAAGACTAATATAAATTTTGTGAGAATTGAGAGGACAAGATTTTTAAGCTTTATTGAAAGTTGGAGGACTAGATTTCAACATTTGAAAGGACTAAAATGAAATGAAACTCAAACTACTAGACCAAAATGATATTTTAAAAGCTTACATTTTCGTTCTTGATGTTAGGGTGGTATTTTATTTTAGCCACATGAATTAAAAAAAATAGTTGACATTGTAAATTAATTCAAATGTGAAAAATTATTCGAAGAGATGTTTAGAATGTGGCTGACAATTTTATACTCTCAGATATAAGGAGAGATTAATAGTTATCACACATTAAATTTGTATCCAATGATTACTAAGGGGTATATAGATGTTCGAATCTACACTATTGAATAATTTCTCTCCCACTTCAAGCCCTGTCACCGTTGGCCTCTTGTCTTCTCTCTTCTCCTCCCTGATACCACCAGCCTCCACCGTCGTGCCATCCACTAAATGGTTGAAGATTTTCCAGATCTTAAAAAACAAAAAAAAAAAACAAAAAAACAAAAAAAACTTCAGATTTATTGTTGAAAGAAACTAGTCGAGAGAGGGAGACCAAAGAGGTAGATGGAGAGATAGAGGGGGGTGGGTGTGTGGATGGCAGGGAAAAATAAATGATATATATCTATATTTTCAAATTTTAAAGACTAAGACTCGTAAGATAATAGTTTGTTTATTAATTTTTAGTTCTTAAAAATTAAGTGTATATACTATTTCCATTATGAATTTCCTTATATATGTTTTCCAAAACCAACTTATGCTTTGAATATTACAAAAATGTAAACTTCATTTCATAACTATTTGAACCCTCTTTGATAACCATTTGGTTTTTGAAAGTTAAACTTATAAATATTACTCTCATCTCTACGGGTTTCCATGTTCCTAGCTAGTTTTTACCTATATTTTCAAAAACCAAGCAAAGTGTTAAAAACTAAAAAAAAATAGTTTCCAACATCTTATTTTTATTTTTAGAATTTGAGTAGAAATTCAAATATTTCTTTAAGAAAAATGGAAATTATTATAGAGAAGATGAAAATTATTAAAAGAAATTGAGAAATTAATGACAATTTTCAAAAACCAGAAACCAAAAACCAAATGGTTATGAAACGTGGTCTTGGTTTCTGGTTTTTTGTTTTTTATTTTTAAAAATTAAACCTATAAATACTACTTCCACCTATGGGTTTTTATGTTTTCTTATCTACTTTCTACATGTGTTTTAAAAAATAAAGTCAAGTTTTTTGTTTTTTGTTTTTTAAAACACATCTACTTTCTACATGTGTTTTAAAAAACAAAGTCAAGGTTTCTGGTTTTTTGTTTTTTATTTTTAAAAATTAAACCTATAAATACTACTTCCACCTATGGGTTTTTATGTTTTCTTATCTACTTTCTACATGTGTTTTAAAAAACAAAGTCAAGTTTTGAAAACTAAAAAAAGTAGTTCCCAAAACTTGTTTTGTTTTGGAATTTGGCTAGAAACTCAAAATGTCTTCGTAATAAAGATGGAAACTTAAAGATGGAAACTATTGTAGAGAAGATTAAAACCAATATGGTAGAGAACTTATAAGAATGCAAGCACAATTAATCTGACCATGTTGATATAATTAAATTTACTCCATCCTATCTGCTTAAACTTTTAGGTTGATTGGTGAATTAAGAAAATTTTCAAAAACCTAAAAACTTATGCGCCGCTTGATAACTATTTTTTTTTTTTTTGTTTTTTAAAATTAAGCTTGTTTTCTTCTCATTTATCTACAATAGTTTTCATCTACTTTAAGGAAACATTTTGAATTCTTAGCCAAATTCTAAATTCAAAAACAAGTTTTTGAAAACTACTTTTTTAGTTTCCAAAACATGGCAAAAAACATAGTTAAAAAGAAGATAACAAAAAAGTAAATCTATAGGTGAAAGTAGTATTTATAGATTTAATATTAAAAACGAAACATAAAAAACTAAATGGTTATTGAATAGAGTCCTAATTGATTATCAAATGGGGCGTAGTTTTTAAAAACTCGCTTTTATTTTTAAAATTTGGGTTAGAATTCAAATGTGTTCTTAATAAATATGAAAGCTATTTTAAAAAATTGTTGAGAAATGATGAGATAAGAATTGTAGAGAGATGATGAGAGAATAAACACAATTTTTAAAAAAAATAAAAAACAAAGAACCAAACAATATTTGATGAAGCCTAAAGTATAAGATTTTAAAAAATAGGAACTAAATGAAGCTATTACCATGAATATGGTAACCAAATGAAGCTATTCAAAAAATCTCATAGACCAAAAAGATAGTTTTCTCTTTATTTTAATTTTGTTTAAAATAGGTGGTTAGTTTTTTTTTTTTTCATTTCTAAAATTTAATTAGGACTTCAAGAATATTTTTAATTTACATTTGTGCCAACGACAAATTTACTAATATCATATAACCCAATTTATTTTAATTAGGTATCATTTGTTAATAAGAAATATTTTTATCAAAATACTAAAATTTAACAATATTTTATATTAATGATTGCAGTTCTAATAAAAGTTTTATAAATATTTGTAGTTGAAGTATTTTTATTTTAATTAGTTACTATATGTTAACGTCCAATAAATTGTCAAAATAAAACATTTCTTCTATCAAATAAAATAGTAATAATTTTTTATTAAAAGAAAAGAGAGAAATTGTATCATACATTATTGTTATATATTAATTAAAAATGATACTAATACCATACTATTTAGTTGATGGAATTTTAAATTAGTTAGAAATTTGAAAGGGACATCTTAGCAATTATTATTATCTCATCTCAATTCTAATTCTAGATGGTTTCTAGTAAACAAAATCAACGTACATCTAATTAGGATTATATTCCATGCAATTTTTAGTAAACAAGATCAAAAGGAATAATACTTTCATTTTGATTTGACTTTCATCCTAATTTCTTGTCATTTGTTTTTAATTATAAGAGTGATTTTAAATACAGGAAAAGAGATTTTACGAATTTCAAAATTACTCTCAAATATGTTGAACCTTTTAAATTGGATTTTGAGTTATAACAGGAGGAGATAAGGGAGGGTTTAGAAGATGGAATCAAGTGGCCAAAATGGATGTCAATTAGCGACCAGAGGCTTTTGGAGTCATCATCAGAAGCGGCGGCGGCGGACGTGGTGGTGGCGGCGGATGGAAGTGGAAATTTTAGAACGGTGTCGGAGGCAGTGGCGGCGGCGCCGAATAAGAGCAGCAAAAGATATGTAATAAGAATAAAGGCTGGAGTTTACAGGGAAAATGTTGAAGTCCCAAGTAATAAGAAGAATATTATGTTTTGGGGAGATGGAAGATCCAATACCTTTATCACAGCTAGCAGAAGTAATGGGACTGGCTGGTCAACCTTCGATTCCGCCACTGTCGGTAAACCAACTAACTTATTTTTAAACTAGTGCTTTCTTACAAACCACTTTAAAATGATTTTTTTTTCTTTAATGTACAAACCGTTTTTATTATTAATTTTTGTGATTTAAAAAAATATATTTTTAAACCAACAAGAATATAGTTCAACTGACATTAGTCATGAGTTCGAATCTCTCGCCTCCAAATGTTAATGTACTAAAAAAACAGAGTGTTTTTAATAGATTAGAAGGCCATTATTATGATTTTTTTTTTAAAAAAAAGAAATAACCAAGGACTATCCATTTAAAAAAAAATACATTGTTTTTGAAAATGTGACCACATCTTGTTTTTTATCAGCAAACTTTTAAAATTATGTTTAATAAGCTTCTAAATTTTATAGAGTATCTAAATAGAGCTTCTTGATCCTTCAAAATATTACTTCTAATAGATTGTCTAATTGGTTACTAAGTTGTTAATTTTGTGTCTAATAAATTTTAAAAACTTTATAAATTGTCTTAGAAATTAAGAACCTATATACAGAATTAAAAATTCAAAAACTTATAGACAATTTTGTGTTAGGGACAACGACCTTCCACATATCTCCACATTGGTATGATATTGTCCACTTTAGGCATAAACCCTCATGGCTTTACTTTTGGTTTCACCCAAAAGGCCTCATACCAATGGAGATACTATCTCTCACTTATATACTCATGATCTCTCCCTTCTCTAGTCGATGTGGGACTTTTGGTCGCACTCCCAACAATTTGAAAGTTTAAAGAACCTTCATTAAGAGAAATTATTCAATGAGGCCGCCACAATGTGGCCGAGTCGTAATAACCATTAGATCTGAATCCAATAATTTTCATGCCCCTTAATATCCTTCGGATATAAATTCGGAGCACTTCTCTCTCTCCATCCAACGGTTACATCAAAAGCTAAGACTTGTTACACTCTAAAATTCAAAAACATAAAAAACAATTATGAAAGTTTAGAGCGTTCTCCCAATTTTACTTCATCTTTTAAAAAATTTCTCAAACCATTTTTTACTATTGAAAATTTTTATTAAAAGAAATGTATTAAAAGGGTTGAGTAGTTTAATTTTTGTTTATACCATTCTATAAAATTTTAACAATTCACTCATTTCGAAAAATGATTCCACGAATCAACATTGAATAATCACTAGATATAAACTCGAGTATTTTCCATCTATATCCAATAATAAACAAACACTTGTTTTTGTTGGCAGTTGCGGTGGGGGATGGATTCTTAGCTCGAGACATAACCATTGAAAACAAAGCAGGAGCAGCCAATCACCAAGCCGTAGCTCTCCGCGTTGGGTCAGATCACTCAGCCTTCTATCGTTGTGGCATTCAAGCCTACCAAGACACACTCTACGTTCACTCCAACCGCCAATTCTTCGTGAACTGTGTAATTGCAGGCACAATCGACTTCATATTCGGAAACGCAGCAGTAGTAATTCAAGACTCAGTGCTCACCCCTCGTCGACCAGACCCTAACCAACAGAACATGGTAACAGCACAAGGAAGAACCGAGGCCAATCAAAACACGGGAATTGTGATCCAAAAGTGCACGATTAAAGCAACCTCGGACTTAGAGCCAGTGATAAAAGAATTCCCAAGTTTCCTTGGTAGACCATGGAAGGAATATGCTAGGGTTGTGGTTATGCAAACAGATATTACCAATGTGATTGACCCAAAAGGGTGGGCGCCATGGAGTGGTGATTTTGCTCTTAAGACACTCTTCTTTGCAGAATATGACAACAAGGGAGATGGAGCCAATACATGTTGTAGGGTGGAGTGGTCCTCGCTTATTAATGCTGATCAAGCTAAGGCTTTTACGGCTGGACAATTCATTAGTGGTGCCGATTGGCTCCCATCCACTGCCTTCCCCTATCAGCTTGGCCTCTAAATCCACCACTCCTTATCCAACTCATCTTCCTTCCTTTTAATTATCTTCATCTACTATATATTTCATGTATTTTTATTCAATAATCTAAAAGCGAAGTGAGGATTGGGAGAAAAAAGTGGGAATGTAATGTTTGTGTTTAAGAATGAGTGGTGTAAGTTAAATAAAGAGAATTTGTAATGGGTAGCAATTATGGTTTTAGTATTTGTAGATATAGCAACATTTTAAAAGAATTGCAAATATGGCAAAAGTCTTTAATTTTTTTATATAATTCCAATTATACCCTTGGCTTAAA

mRNA sequence

CACCAACCTATCTGCCTTTCTTCACAACTCTTCTCATTTGCGAGCTATGGCAAACACTAAAGTTTCATTGAAGAAAAAGAATGAGCTTCTTGTCTTAGCTCTCTTTGCTACTTTACTCACAAATGCTATTGTTGTCATTTCTGAATTCAATTATGATGAGAATAGTCTTAACAATGTGGGTATTGTAGAACACTCATGTGCCACCACTCTTTATCCACAACTATGTCATTCAACAATATCCTCCATCGTGGGAGCATCCGAGTTTTTGAGTGTAAAAGATATTGTTGAAATCTCGTTGAGTGTTGCTATAGATGCAGCTAAGTATAACCATGAAAACATCAAGAAACTTTCGATGTCGCTCAATGATCTTACTAAACGAGAGAGAATCACTCTCCGTGATTGTATCGTGACCACCGACAAGACGCTCGAAGACCTTGACAAGGTCGTAGAGGATCTTCGTGAGTATCCAAATAAGAAATCTATTATCCTATATGCTGATGACCTCAAAACGTTTCTCAGTTCTGCCATGACCAACCAGGAGACTTGTTTGGATGGATTCTCAGACGAGGAAGCTGAAAAAAGGGTGTTGAAATTGATTGAAACCCCACATATTCACGTGACAAAGTTGTGTAGTAATGTTCTAGCCCTGGTCAAGAATTTGACTGACACTGATTTAACTTTAAGGGGCTTGGTCCCTGAGGACCCTAATAAGATAGCTTCATCCTGGCATGCTTACGAATCCGACCCCAAAATTGTACTCTTTTCAGACCTTACTAACGAGGAGGAGATAAGGGAGGGTTTAGAAGATGGAATCAAGTGGCCAAAATGGATGTCAATTAGCGACCAGAGGCTTTTGGAGTCATCATCAGAAGCGGCGGCGGCGGACGTGGTGGTGGCGGCGGATGGAAGTGGAAATTTTAGAACGGTGTCGGAGGCAGTGGCGGCGGCGCCGAATAAGAGCAGCAAAAGATATGTAATAAGAATAAAGGCTGGAGTTTACAGGGAAAATGTTGAAGTCCCAAGTAATAAGAAGAATATTATGTTTTGGGGAGATGGAAGATCCAATACCTTTATCACAGCTAGCAGAAGTAATGGGACTGGCTGGTCAACCTTCGATTCCGCCACTGTCGTTGCGGTGGGGGATGGATTCTTAGCTCGAGACATAACCATTGAAAACAAAGCAGGAGCAGCCAATCACCAAGCCGTAGCTCTCCGCGTTGGGTCAGATCACTCAGCCTTCTATCGTTGTGGCATTCAAGCCTACCAAGACACACTCTACGTTCACTCCAACCGCCAATTCTTCGTGAACTGTGTAATTGCAGGCACAATCGACTTCATATTCGGAAACGCAGCAGTAGTAATTCAAGACTCAGTGCTCACCCCTCGTCGACCAGACCCTAACCAACAGAACATGGTAACAGCACAAGGAAGAACCGAGGCCAATCAAAACACGGGAATTGTGATCCAAAAGTGCACGATTAAAGCAACCTCGGACTTAGAGCCAGTGATAAAAGAATTCCCAAGTTTCCTTGGTAGACCATGGAAGGAATATGCTAGGGTTGTGGTTATGCAAACAGATATTACCAATGTGATTGACCCAAAAGGGTGGGCGCCATGGAGTGGTGATTTTGCTCTTAAGACACTCTTCTTTGCAGAATATGACAACAAGGGAGATGGAGCCAATACATGTTGTAGGGTGGAGTGGTCCTCGCTTATTAATGCTGATCAAGCTAAGGCTTTTACGGCTGGACAATTCATTAGTGGTGCCGATTGGCTCCCATCCACTGCCTTCCCCTATCAGCTTGGCCTCTAAATCCACCACTCCTTATCCAACTCATCTTCCTTCCTTTTAATTATCTTCATCTACTATATATTTCATGTATTTTTATTCAATAATCTAAAAGCGAAGTGAGGATTGGGAGAAAAAAGTGGGAATGTAATGTTTGTGTTTAAGAATGAGTGGTGTAAGTTAAATAAAGAGAATTTGTAATGGGTAGCAATTATGGTTTTAGTATTTGTAGATATAGCAACATTTTAAAAGAATTGCAAATATGGCAAAAGTCTTTAATTTTTTTATATAATTCCAATTATACCCTTGGCTTAAA

Coding sequence (CDS)

ATGGCAAACACTAAAGTTTCATTGAAGAAAAAGAATGAGCTTCTTGTCTTAGCTCTCTTTGCTACTTTACTCACAAATGCTATTGTTGTCATTTCTGAATTCAATTATGATGAGAATAGTCTTAACAATGTGGGTATTGTAGAACACTCATGTGCCACCACTCTTTATCCACAACTATGTCATTCAACAATATCCTCCATCGTGGGAGCATCCGAGTTTTTGAGTGTAAAAGATATTGTTGAAATCTCGTTGAGTGTTGCTATAGATGCAGCTAAGTATAACCATGAAAACATCAAGAAACTTTCGATGTCGCTCAATGATCTTACTAAACGAGAGAGAATCACTCTCCGTGATTGTATCGTGACCACCGACAAGACGCTCGAAGACCTTGACAAGGTCGTAGAGGATCTTCGTGAGTATCCAAATAAGAAATCTATTATCCTATATGCTGATGACCTCAAAACGTTTCTCAGTTCTGCCATGACCAACCAGGAGACTTGTTTGGATGGATTCTCAGACGAGGAAGCTGAAAAAAGGGTGTTGAAATTGATTGAAACCCCACATATTCACGTGACAAAGTTGTGTAGTAATGTTCTAGCCCTGGTCAAGAATTTGACTGACACTGATTTAACTTTAAGGGGCTTGGTCCCTGAGGACCCTAATAAGATAGCTTCATCCTGGCATGCTTACGAATCCGACCCCAAAATTGTACTCTTTTCAGACCTTACTAACGAGGAGGAGATAAGGGAGGGTTTAGAAGATGGAATCAAGTGGCCAAAATGGATGTCAATTAGCGACCAGAGGCTTTTGGAGTCATCATCAGAAGCGGCGGCGGCGGACGTGGTGGTGGCGGCGGATGGAAGTGGAAATTTTAGAACGGTGTCGGAGGCAGTGGCGGCGGCGCCGAATAAGAGCAGCAAAAGATATGTAATAAGAATAAAGGCTGGAGTTTACAGGGAAAATGTTGAAGTCCCAAGTAATAAGAAGAATATTATGTTTTGGGGAGATGGAAGATCCAATACCTTTATCACAGCTAGCAGAAGTAATGGGACTGGCTGGTCAACCTTCGATTCCGCCACTGTCGTTGCGGTGGGGGATGGATTCTTAGCTCGAGACATAACCATTGAAAACAAAGCAGGAGCAGCCAATCACCAAGCCGTAGCTCTCCGCGTTGGGTCAGATCACTCAGCCTTCTATCGTTGTGGCATTCAAGCCTACCAAGACACACTCTACGTTCACTCCAACCGCCAATTCTTCGTGAACTGTGTAATTGCAGGCACAATCGACTTCATATTCGGAAACGCAGCAGTAGTAATTCAAGACTCAGTGCTCACCCCTCGTCGACCAGACCCTAACCAACAGAACATGGTAACAGCACAAGGAAGAACCGAGGCCAATCAAAACACGGGAATTGTGATCCAAAAGTGCACGATTAAAGCAACCTCGGACTTAGAGCCAGTGATAAAAGAATTCCCAAGTTTCCTTGGTAGACCATGGAAGGAATATGCTAGGGTTGTGGTTATGCAAACAGATATTACCAATGTGATTGACCCAAAAGGGTGGGCGCCATGGAGTGGTGATTTTGCTCTTAAGACACTCTTCTTTGCAGAATATGACAACAAGGGAGATGGAGCCAATACATGTTGTAGGGTGGAGTGGTCCTCGCTTATTAATGCTGATCAAGCTAAGGCTTTTACGGCTGGACAATTCATTAGTGGTGCCGATTGGCTCCCATCCACTGCCTTCCCCTATCAGCTTGGCCTCTAA

Protein sequence

MANTKVSLKKKNELLVLALFATLLTNAIVVISEFNYDENSLNNVGIVEHSCATTLYPQLCHSTISSIVGASEFLSVKDIVEISLSVAIDAAKYNHENIKKLSMSLNDLTKRERITLRDCIVTTDKTLEDLDKVVEDLREYPNKKSIILYADDLKTFLSSAMTNQETCLDGFSDEEAEKRVLKLIETPHIHVTKLCSNVLALVKNLTDTDLTLRGLVPEDPNKIASSWHAYESDPKIVLFSDLTNEEEIREGLEDGIKWPKWMSISDQRLLESSSEAAAADVVVAADGSGNFRTVSEAVAAAPNKSSKRYVIRIKAGVYRENVEVPSNKKNIMFWGDGRSNTFITASRSNGTGWSTFDSATVVAVGDGFLARDITIENKAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQFFVNCVIAGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRTEANQNTGIVIQKCTIKATSDLEPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEYDNKGDGANTCCRVEWSSLINADQAKAFTAGQFISGADWLPSTAFPYQLGL
Homology
BLAST of Tan0007352 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 6.7e-166
Identity = 315/593 (53.12%), Postives = 409/593 (68.97%), Query Frame = 0

Query: 7   SLKKKNELLVLALFATLLTNAIV--VISEFNYDENSLNN-----VGIVEHSCATTLYPQL 66
           ++ KK + L LALFATLL  A V  +++  N  +NS +N       I++ SC++T YP L
Sbjct: 22  NIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDL 81

Query: 67  CHSTISSIVGAS-EFLSVKDIVEISLSVAIDAAKYNHENIKKLSMSLNDLTKRERITLRD 126
           C S I+++  AS +  S KD++E+SL++   A ++N+  I+KL +   +LTKRE++ L D
Sbjct: 82  CFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKL-LKRTNLTKREKVALHD 141

Query: 127 CIVTTDKTLEDLDKVVEDLREYPNKKSIILYADDLKTFLSSAMTNQETCLDGFSDEEAEK 186
           C+ T D+TL++L K VEDL EYPNKKS+  +ADDLKT +S+AMTNQ TCLDGFS ++A K
Sbjct: 142 CLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANK 201

Query: 187 RVLKLIETPHIHVTKLCSNVLALVKNLTDTDLTLRGLVPEDPNKIASSWHAYESDPKIVL 246
            V   +    +HV K+CSN LA++KN+TDTD+                           +
Sbjct: 202 HVRDALSDGQVHVEKMCSNALAMIKNMTDTDM---------------------------M 261

Query: 247 FSDLTNEEEIREGLEDGIKWPKWMSISDQRLLESSSEAAAADVVVAADGSGNFRTVSEAV 306
               +N  ++ E       WP W+S  D+RLL+SSS     +VVVAADGSGNF+TV+ +V
Sbjct: 262 IMRTSNNRKLIEETSTVDGWPAWLSTGDRRLLQSSS--VTPNVVVAADGSGNFKTVAASV 321

Query: 307 AAAPNKSSKRYVIRIKAGVYRENVEVPSNKKNIMFWGDGRSNTFITASRSNGTGWSTFDS 366
           AAAP   +KRY+IRIKAGVYRENVEV    KNIMF GDGR+ T IT SR+   G +TF S
Sbjct: 322 AAAPQGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKS 381

Query: 367 ATVVAVGDGFLARDITIENKAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQF 426
           ATV  VG+GFLARDIT +N AG + HQAVALRVG+D SAFY C + AYQDTLYVHSNRQF
Sbjct: 382 ATVAVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQF 441

Query: 427 FVNCVIAGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRTEANQNTGIVIQKCTI 486
           FVNC+IAGT+DFIFGNAA V+Q+  +  R+P+  Q+NMVTAQGR + NQNTGIVIQK  I
Sbjct: 442 FVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRI 501

Query: 487 KATSDLEPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEY 546
            ATSDL+PV   FP++LGRPWKEY+R V+MQ+ IT+VI P GW  W G+FAL TLF+ E+
Sbjct: 502 GATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEH 561

Query: 547 DNKGDGANTCCRVEWSS---LINADQAKAFTAGQFISGADWLPSTAFPYQLGL 589
            N G GA T  RV+W     + +A +A+AFT G FI+G+ WL ST FP+ LGL
Sbjct: 562 QNAGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Tan0007352 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 1.2e-165
Identity = 314/593 (52.95%), Postives = 408/593 (68.80%), Query Frame = 0

Query: 7   SLKKKNELLVLALFATLLTNAIV--VISEFNYDENSLNN-----VGIVEHSCATTLYPQL 66
           ++ KK + L LALFATLL  A V  +++  N  +NS +N       I++ SC++T YP L
Sbjct: 22  NIPKKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNGNEPHHAILKSSCSSTRYPDL 81

Query: 67  CHSTISSIVGAS-EFLSVKDIVEISLSVAIDAAKYNHENIKKLSMSLNDLTKRERITLRD 126
           C S I+++  AS +  S KD++E+SL++   A ++N+  I+KL +   +LTKRE++ L D
Sbjct: 82  CFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKL-LKRTNLTKREKVALHD 141

Query: 127 CIVTTDKTLEDLDKVVEDLREYPNKKSIILYADDLKTFLSSAMTNQETCLDGFSDEEAEK 186
           C+ T D+TL++L K VEDL EYPNKKS+  +ADDLKT +S+AMTNQ TCLDGFS ++A K
Sbjct: 142 CLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDDANK 201

Query: 187 RVLKLIETPHIHVTKLCSNVLALVKNLTDTDLTLRGLVPEDPNKIASSWHAYESDPKIVL 246
            V   +    +HV K+CSN LA++KN+TDTD+                           +
Sbjct: 202 HVRDALSDGQVHVEKMCSNALAMIKNMTDTDM---------------------------M 261

Query: 247 FSDLTNEEEIREGLEDGIKWPKWMSISDQRLLESSSEAAAADVVVAADGSGNFRTVSEAV 306
               +N  ++ E       WP W+S  D+RLL+SSS     + VVAADGSGNF+TV+ AV
Sbjct: 262 IMRTSNNRKLTEETSTVDGWPAWLSPGDRRLLQSSS--VTPNAVVAADGSGNFKTVAAAV 321

Query: 307 AAAPNKSSKRYVIRIKAGVYRENVEVPSNKKNIMFWGDGRSNTFITASRSNGTGWSTFDS 366
           AAAP   +KRY+IRIKAGVYRENVEV    KNIMF GDGR+ T IT SR+   G +TF S
Sbjct: 322 AAAPQGGTKRYIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKS 381

Query: 367 ATVVAVGDGFLARDITIENKAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQF 426
           AT   VG+GFLARDIT +N AG + HQAVALRVG+D SAFY C + AYQDTLYVHSNRQF
Sbjct: 382 ATAAVVGEGFLARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQF 441

Query: 427 FVNCVIAGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRTEANQNTGIVIQKCTI 486
           FVNC+IAGT+DFIFGNAA V+Q+  +  R+P+  Q+NMVTAQGRT+ NQNTGIVIQK  I
Sbjct: 442 FVNCLIAGTVDFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRI 501

Query: 487 KATSDLEPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEY 546
            ATSDL+PV   FP++LGRPWKEY+R V+MQ+ IT++I P GW  W G+FAL TLF+ E+
Sbjct: 502 GATSDLKPVQGSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEH 561

Query: 547 DNKGDGANTCCRVEWSS---LINADQAKAFTAGQFISGADWLPSTAFPYQLGL 589
            N G GA T  RV+W     + +A +A+AFT G FI+G+ WL ST FP+ LGL
Sbjct: 562 QNSGAGAGTSGRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

BLAST of Tan0007352 vs. ExPASy Swiss-Prot
Match: Q43143 (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 1.8e-163
Identity = 312/596 (52.35%), Postives = 410/596 (68.79%), Query Frame = 0

Query: 9   KKKNELLVLALFATLLTNAI--VVISEFNYDENSLNNV----------GIVEHSCATTLY 68
           K+K ++ +  + + LL  A+  VV    ++ +NS ++            IV+ +C+ TL+
Sbjct: 16  KRKKKIYLAIVASVLLVAAVIGVVAGVKSHSKNSDDHADIMAISSSAHAIVKSACSNTLH 75

Query: 69  PQLCHSTISSIVGAS-EFLSVKDIVEISLSVAIDAAKYNHENIKKLSMSLNDLTKRERIT 128
           P+LC+S I ++   S +  S KD++E+SL++ + A + N+  +K+L  +   LT RE++ 
Sbjct: 76  PELCYSAIVNVSDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKELIKTRKGLTPREKVA 135

Query: 129 LRDCIVTTDKTLEDLDKVVEDLREYPNKKSIILYADDLKTFLSSAMTNQETCLDGFSDEE 188
           L DC+ T D+TL++L   VEDL  YPNKKS+  + +DLKT +SSA+TNQETCLDGFS +E
Sbjct: 136 LHDCLETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLISSAITNQETCLDGFSHDE 195

Query: 189 AEKRVLKLIETPHIHVTKLCSNVLALVKNLTDTDLTLRGLVPEDPNKIASSWHAYESDPK 248
           A+K+V K++     HV K+CSN LA++ N+TDTD+          N++  S  A      
Sbjct: 196 ADKKVRKVLLKGQKHVEKMCSNALAMICNMTDTDIA---------NEMKLSAPANN---- 255

Query: 249 IVLFSDLTNEEEIREGLEDGIKWPKWMSISDQRLLESSSEAAAADVVVAADGSGNFRTVS 308
                        R+ +ED  +WP+W+S  D+RLL+SS+     DVVVAADGSG+++TVS
Sbjct: 256 -------------RKLVEDNGEWPEWLSAGDRRLLQSST--VTPDVVVAADGSGDYKTVS 315

Query: 309 EAVAAAPNKSSKRYVIRIKAGVYRENVEVPSNKKNIMFWGDGRSNTFITASRSNGTGWST 368
           EAV  AP KSSKRYVIRIKAGVYRENV+VP  K NIMF GDG+SNT ITASR+   G +T
Sbjct: 316 EAVRKAPEKSSKRYVIRIKAGVYRENVDVPKKKTNIMFMGDGKSNTIITASRNVQDGSTT 375

Query: 369 FDSATVVAVGDGFLARDITIENKAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSN 428
           F SATVV V    LARDIT +N AGA+ HQAVAL VGSD SAFYRC + AYQDTLYVHSN
Sbjct: 376 FHSATVVRVAGKVLARDITFQNTAGASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVHSN 435

Query: 429 RQFFVNCVIAGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRTEANQNTGIVIQK 488
           RQFFV C++AGT+DFIFGN A V QD  +  RRP   Q+NMVTAQGRT+ NQNTGIVIQK
Sbjct: 436 RQFFVQCLVAGTVDFIFGNGAAVFQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQK 495

Query: 489 CTIKATSDLEPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFF 548
           C I ATSDL PV K FP++LGRPWKEY+R V+MQ+ IT+VI P GW  W+G+FAL TLF+
Sbjct: 496 CRIGATSDLRPVQKSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDTLFY 555

Query: 549 AEYDNKGDGANTCCRVEWSS---LINADQAKAFTAGQFISGADWLPSTAFPYQLGL 589
            EY N G GA T  RV+W     + ++ +A+A+T G+FI+G  WL ST FP+ LGL
Sbjct: 556 GEYANTGAGAPTSGRVKWKGHKVITSSTEAQAYTPGRFIAGGSWLSSTGFPFSLGL 583

BLAST of Tan0007352 vs. ExPASy Swiss-Prot
Match: O49006 (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)

HSP 1 Score: 570.5 bits (1469), Expect = 2.2e-161
Identity = 314/590 (53.22%), Postives = 400/590 (67.80%), Query Frame = 0

Query: 10  KKNELLVL--ALFATLLTNAIVVISEFNYDEN-----SLNNVGIVEHSCATTLYPQLCHS 69
           KKN+ LVL  A  A L   A+  IS      N     S ++  ++  SC++T YP+LC S
Sbjct: 15  KKNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCIS 74

Query: 70  TISSIVGASEFLSVKDIVEISLSVAIDAAKYNHENIKKLSMSLNDLTKRERITLRDCIVT 129
            + +  G  E  S KD++E S+++ I A ++N+  +KKL      LT RE+  L DC+ T
Sbjct: 75  AVVT-AGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLET 134

Query: 130 TDKTLEDLDKVVEDLREYPNKKSIILYADDLKTFLSSAMTNQETCLDGFSDEEAEKRVLK 189
            D+TL++L + VEDL  YP KK++  +A DLKT +SSA+TNQETCLDGFS ++A+K+V K
Sbjct: 135 IDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRK 194

Query: 190 LIETPHIHVTKLCSNVLALVKNLTDTDLTLRGLVPEDPNKIASSWHAY-ESDPKIVLFSD 249
            +    IHV  +CSN LA++KN+TDTD+       E   KI S+     E + +  +  D
Sbjct: 195 ALLKGQIHVEHMCSNALAMIKNMTDTDIANF----EQKAKITSNNRKLKEENQETTVAVD 254

Query: 250 LTNEEEIREGLEDGIKWPKWMSISDQRLLESSSEAAAADVVVAADGSGNFRTVSEAVAAA 309
           +    E+     D   WP W+S  D+RLL+ S     AD  VAADGSG F+TV+ AVAAA
Sbjct: 255 IAGAGEL-----DSEGWPTWLSAGDRRLLQGS--GVKADATVAADGSGTFKTVAAAVAAA 314

Query: 310 PNKSSKRYVIRIKAGVYRENVEVPSNKKNIMFWGDGRSNTFITASRSNGTGWSTFDSATV 369
           P  S+KRYVI IKAGVYRENVEV   KKNIMF GDGR+ T IT SR+   G +TF SATV
Sbjct: 315 PENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATV 374

Query: 370 VAVGDGFLARDITIENKAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQFFVN 429
            AVG+ FLARDIT +N AG + HQAVALRVGSD SAFY C + AYQDTLYVHSNRQFFV 
Sbjct: 375 AAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVK 434

Query: 430 CVIAGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRTEANQNTGIVIQKCTIKAT 489
           C+IAGT+DFIFGNAAVV+QD  +  RRP+  Q+NMVTAQGRT+ NQNTGIVIQKC I AT
Sbjct: 435 CLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGAT 494

Query: 490 SDLEPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEYDNK 549
           SDL+ V   FP++LGRPWKEY++ V+MQ+ I++VI P+GW+ W+G FAL TL + EY N 
Sbjct: 495 SDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNT 554

Query: 550 GDGANTCCRVEWSS---LINADQAKAFTAGQFISGADWLPSTAFPYQLGL 589
           G GA T  RV+W     +  A +A+ +TAGQFI G  WL ST FP+ LGL
Sbjct: 555 GAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of Tan0007352 vs. ExPASy Swiss-Prot
Match: Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)

HSP 1 Score: 557.0 bits (1434), Expect = 2.6e-157
Identity = 310/600 (51.67%), Postives = 402/600 (67.00%), Query Frame = 0

Query: 4   TKVSLKKKNELLVL--ALFATLLTNAIVVISEFNYDEN--------SLNNVGIVEHSCAT 63
           +K S  K N+ L+L  A  A LL  +IV I+    ++N        S  +  I++  C++
Sbjct: 9   SKFSDFKNNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSS 68

Query: 64  TLYPQLCHSTISSIVGASEFLSVKDIVEISLSVAIDAAKYNHENIKKLSMSLNDLTKRER 123
           TLYP+LC S +++  G  E  S K+++E SL++   A K+N+  +KKL      LT RE 
Sbjct: 69  TLYPELCFSAVAA-TGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREV 128

Query: 124 ITLRDCIVTTDKTLEDLDKVVEDLREYPNKKSIILYADDLKTFLSSAMTNQETCLDGFSD 183
             L DC+ T D+TL++L   VEDL +YP +KS+  +ADDLKT +SSA+TNQ TCLDGFS 
Sbjct: 129 TALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSY 188

Query: 184 EEAEKRVLKLIETPHIHVTKLCSNVLALVKNLTDTDLTLRGLVPEDPNKIASSWHAYESD 243
           ++A+++V K +    +HV  +CSN LA++KN+T+TD+                   +E  
Sbjct: 189 DDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIA-----------------NFELR 248

Query: 244 PKIVLFSDLTNEEEIRE--GLEDGIKWPKWMSISDQRLLESSSEAAAADVVVAADGSGNF 303
            K   F++  N  +++E  G  D   WPKW+S+ D+RLL+ S+    AD  VA DGSG+F
Sbjct: 249 DKSSTFTN-NNNRKLKEVTGDLDSDGWPKWLSVGDRRLLQGST--IKADATVADDGSGDF 308

Query: 304 RTVSEAVAAAPNKSSKRYVIRIKAGVYRENVEVPSNKKNIMFWGDGRSNTFITASRSNGT 363
            TV+ AVAAAP KS+KR+VI IKAGVYRENVEV   K NIMF GDGR  T IT SR+   
Sbjct: 309 TTVAAAVAAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVD 368

Query: 364 GWSTFDSATVVAVGDGFLARDITIENKAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLY 423
           G +TF SATV AVG+ FLARDIT +N AG + HQAVALRVGSD SAFY+C + AYQDTLY
Sbjct: 369 GSTTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLY 428

Query: 424 VHSNRQFFVNCVIAGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRTEANQNTGI 483
           VHSNRQFFV C I GT+DFIFGNAA V+QD  +  RRP+  Q+NMVTAQGR++ NQNTGI
Sbjct: 429 VHSNRQFFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGI 488

Query: 484 VIQKCTIKATSDLEPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALK 543
           VIQ C I  TSDL  V   FP++LGRPWKEY+R V+MQ+DI++VI P+GW  WSG FAL 
Sbjct: 489 VIQNCRIGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALD 548

Query: 544 TLFFAEYDNKGDGANTCCRVEWS--SLINAD-QAKAFTAGQFISGADWLPSTAFPYQLGL 589
           TL + EY N+G GA T  RV+W    +I +D +A+ FTAGQFI G  WL ST FP+ L L
Sbjct: 549 TLTYREYLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of Tan0007352 vs. NCBI nr
Match: XP_008458040.2 (PREDICTED: LOW QUALITY PROTEIN: pectinesterase-like [Cucumis melo])

HSP 1 Score: 783.1 bits (2021), Expect = 1.7e-222
Identity = 413/584 (70.72%), Postives = 479/584 (82.02%), Query Frame = 0

Query: 11  KNELLVLALFAT-LLTNAIVVISEFNYDENSLNNVGIVEHSCATTLYPQLCHSTISSIVG 70
           +N  L LALFAT LLTNAI+V +  N +    N   ++E SCA TLYPQLCHSTISSI+G
Sbjct: 2   ENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSIIG 61

Query: 71  ASEFLSVKDIVEISLSVAIDAAKYNHENIKKLSMSLNDLTKRERITLRDCIVTTDKTLED 130
            S  LS+KDIV++SLSVA+DAAK N+ NIK L MS N+++KR+RI L DC+ +TD+T+ +
Sbjct: 62  TSNLLSLKDIVQLSLSVAMDAAKLNNGNIKNL-MSFNNVSKRDRIPLHDCVKSTDRTIYE 121

Query: 131 LDKVVEDLREYPNKKSIILYADDLKTFLSSAMTNQETCLDGFSDEEAEKRVLKLIETPHI 190
           LDK +ED REYPNKKS+  YADDLKTFLSSA+TNQ TCLDGF D++ EK VL++IE  HI
Sbjct: 122 LDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTHI 181

Query: 191 HVTKLCSNVLALVKNLTDTDLTL---RGLVPED-PNKIASSWHAYESDPKIVLFSDL-TN 250
           H TKLCSN LALVK LT T++ L   + LV  D P KI +S  +  +D KIVLFS+    
Sbjct: 182 HATKLCSNALALVKKLT-TEIALIDEKNLVVHDFPYKI-TSISSQINDLKIVLFSNQEEG 241

Query: 251 EEEIREGLEDGIKWPKWMSISDQRLLESSSEAAAADVVVAADGSGNFRTVSEAVAAAPNK 310
           E   RE LE+GIKWP+WMSI DQ+LLESSS  AAADVVVA DGSGN++TVSEAV AAP+K
Sbjct: 242 ENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSK 301

Query: 311 SSKRYVIRIKAGVYRENVEVPSNKKNIMFWGDGRSNTFITASRSNGTGWSTFDSATVVAV 370
           +SKRY+I+IKAGVYRENV+VPS+K+NIMFWGDGRSNT ITA RS+G+GWSTF+SATVVAV
Sbjct: 302 NSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAV 361

Query: 371 GDGFLARDITIENKAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQFFVNCVI 430
           GDGFLARDI+ EN AG AN QAVALRVGSDHSAFYRCG+  YQDTLYVHSNRQFFVNCV+
Sbjct: 362 GDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVV 421

Query: 431 AGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRTEANQNTGIVIQKCTIKATSDL 490
           AGT+DF+FGNAA VIQ+S LTPR+P PNQ+NMVTAQ RT+ NQNTGIVIQKC IKATSDL
Sbjct: 422 AGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDL 481

Query: 491 EPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEYDNKGDG 550
           EPVIKEFPSFLGRPW+EYARVVVMQT ITNVID KGW+ W+GD  +K  +FAEYDN G G
Sbjct: 482 EPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYFAEYDNNGAG 541

Query: 551 ANTCCRVEWSSLINADQAKAFTAGQFISGADWLPSTAFPYQLGL 589
           ANT  RV WS +IN   AK FTA +FI GADWLPST FPYQLGL
Sbjct: 542 ANTSGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 580

BLAST of Tan0007352 vs. NCBI nr
Match: TYK30782.1 (pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 780.8 bits (2015), Expect = 8.3e-222
Identity = 407/580 (70.17%), Postives = 472/580 (81.38%), Query Frame = 0

Query: 11  KNELLVLALFAT-LLTNAIVVISEFNYDENSLNNVGIVEHSCATTLYPQLCHSTISSIVG 70
           +N  L LALFAT LLTNAI+V +  N +    N   ++E SCA TLYPQLCHSTISSI+G
Sbjct: 2   ENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSIIG 61

Query: 71  ASEFLSVKDIVEISLSVAIDAAKYNHENIKKLSMSLNDLTKRERITLRDCIVTTDKTLED 130
            S  LS+KDIV++SLSVA+DAAK N+ NIKKL+MS+N+++KR+RI L DC+ +TD+T+ +
Sbjct: 62  TSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSVNNVSKRDRIPLHDCVKSTDRTIYE 121

Query: 131 LDKVVEDLREYPNKKSIILYADDLKTFLSSAMTNQETCLDGFSDEEAEKRVLKLIETPHI 190
           LDK +ED REYPNKKS+  YADDLKTFLSSA+TNQ TCLDGF D++ EKRVL++IE  HI
Sbjct: 122 LDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKRVLRVIENTHI 181

Query: 191 HVTKLCSNVLALVKNLTDTDLTLRGLVPEDPNKIASSWHAYESDPKIVLFSDL-TNEEEI 250
           H TKLCSN LALVK LT T++ L      D   +  S  A       VLFS+    E   
Sbjct: 182 HATKLCSNALALVKKLT-TEIAL-----IDEKNLKKSRKARSH----VLFSNQEEGENGR 241

Query: 251 REGLEDGIKWPKWMSISDQRLLESSSEAAAADVVVAADGSGNFRTVSEAVAAAPNKSSKR 310
           RE LE+GIKWP+WMSI DQ+LLESSS  AA DVVVA DGSGN++TVSEAV AAP+K+SKR
Sbjct: 242 REDLENGIKWPEWMSIQDQKLLESSSSEAAVDVVVAVDGSGNYKTVSEAVEAAPSKNSKR 301

Query: 311 YVIRIKAGVYRENVEVPSNKKNIMFWGDGRSNTFITASRSNGTGWSTFDSATVVAVGDGF 370
           Y+I+IKAGVYRENV+VPS+K+NIMFWGDGRSNT ITA RS+G+GWSTF+SATVVAVGDGF
Sbjct: 302 YIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVGDGF 361

Query: 371 LARDITIENKAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQFFVNCVIAGTI 430
           LARDI+ EN AG AN QAVALRVGSDHSAFYRCG+  YQDTLYVHSNRQFFVNCV+AGT+
Sbjct: 362 LARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVAGTV 421

Query: 431 DFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRTEANQNTGIVIQKCTIKATSDLEPVI 490
           DF+FGNAA VIQ+S LTPR+P PNQ+NMVTAQ RT+ NQNTGIVIQKC IKATSDLEPVI
Sbjct: 422 DFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLEPVI 481

Query: 491 KEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEYDNKGDGANTC 550
           KEFPSFLGRPW+EYARVVVMQT ITNVID KGW+ W+GD  +K  +FAEYDN G GANT 
Sbjct: 482 KEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYFAEYDNNGAGANTS 541

Query: 551 CRVEWSSLINADQAKAFTAGQFISGADWLPSTAFPYQLGL 589
            RV WS +IN   AK FTA +FI GADWLPST FPYQLGL
Sbjct: 542 GRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 569

BLAST of Tan0007352 vs. NCBI nr
Match: KAA0026177.1 (pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 778.9 bits (2010), Expect = 3.2e-221
Identity = 403/579 (69.60%), Postives = 468/579 (80.83%), Query Frame = 0

Query: 11  KNELLVLALFAT-LLTNAIVVISEFNYDENSLNNVGIVEHSCATTLYPQLCHSTISSIVG 70
           +N  L LALFAT LLTNAI+V +  N +    N   ++E SCA TLYPQLCHSTISSI+G
Sbjct: 2   ENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSIIG 61

Query: 71  ASEFLSVKDIVEISLSVAIDAAKYNHENIKKLSMSLNDLTKRERITLRDCIVTTDKTLED 130
            S  LS+KDIV++SLSVA+DAAK N+ NIKKL+MS+N+++KR+RI L DC+ +TD+T+ +
Sbjct: 62  TSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSVNNVSKRDRIPLHDCVKSTDRTIYE 121

Query: 131 LDKVVEDLREYPNKKSIILYADDLKTFLSSAMTNQETCLDGFSDEEAEKRVLKLIETPHI 190
           LDK +ED REYPNKKS+  YADDLKTFLSSA+TNQ TCLDGF D++ EKRVL++IE  HI
Sbjct: 122 LDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKRVLRVIENTHI 181

Query: 191 HVTKLCSNVLALVKNLTDTDLTLRGLVPEDPNKIASSWHAYESDPKIVLFSDLTNEEEIR 250
           H TKLCSN LALVK LT    T   L+ E   K +    ++E             E   R
Sbjct: 182 HATKLCSNALALVKKLT----TEIALIDEKNLKKSRKARSHEE-----------GENGRR 241

Query: 251 EGLEDGIKWPKWMSISDQRLLESSSEAAAADVVVAADGSGNFRTVSEAVAAAPNKSSKRY 310
           E LE+GIKWP+WMSI DQ+LLESSS  AA DVVVA DGSGN++TVSEAV AAP+K+SKRY
Sbjct: 242 EDLENGIKWPEWMSIQDQKLLESSSSEAAVDVVVAVDGSGNYKTVSEAVEAAPSKNSKRY 301

Query: 311 VIRIKAGVYRENVEVPSNKKNIMFWGDGRSNTFITASRSNGTGWSTFDSATVVAVGDGFL 370
           +I+IKAGVYRENV+VPS+K+NIMFWGDGRSNT ITA RS+G+GWSTF+SATVVAVGDGFL
Sbjct: 302 IIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVGDGFL 361

Query: 371 ARDITIENKAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQFFVNCVIAGTID 430
           ARDI+ EN AG AN QAVALRVGSDHSAFYRCG+  YQDTLYVHSNRQFFVNCV+AGT+D
Sbjct: 362 ARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVAGTVD 421

Query: 431 FIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRTEANQNTGIVIQKCTIKATSDLEPVIK 490
           F+FGNAA VIQ+S LTPR+P PNQ+NMVTAQ RT+ NQNTGIVIQKC IKATSDLEPVIK
Sbjct: 422 FVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLEPVIK 481

Query: 491 EFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEYDNKGDGANTCC 550
           EFPSFLGRPW+EYARVVVMQT ITNVID KGW+ W+GD  +K  +FAEYDN G GANT  
Sbjct: 482 EFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYFAEYDNNGAGANTSG 541

Query: 551 RVEWSSLINADQAKAFTAGQFISGADWLPSTAFPYQLGL 589
           RV WS +IN   AK FTA +FI GADWLPST FPYQLGL
Sbjct: 542 RVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 563

BLAST of Tan0007352 vs. NCBI nr
Match: XP_011659194.1 (pectinesterase [Cucumis sativus] >KGN44619.1 hypothetical protein Csa_015815 [Cucumis sativus])

HSP 1 Score: 773.5 bits (1996), Expect = 1.3e-219
Identity = 413/584 (70.72%), Postives = 477/584 (81.68%), Query Frame = 0

Query: 11  KNELLVLALFAT-LLTNAIVVISEFNYDENSLNNVGIVEHSCATTLYPQLCHSTISSIVG 70
           +N  L LALFAT LLTNAI+V +  N +    N + ++E SCA TLYPQLCHSTISSI+G
Sbjct: 2   ENTKLSLALFATLLLTNAIIVRANSNENGVMNNILSVIETSCAITLYPQLCHSTISSILG 61

Query: 71  ASEFLSVKDIVEISLSVAIDAAKYNHENIKKLSMSLNDLTKRERITLRDCIVTTDKTLED 130
            S  LS+KDIVE+SLSVA+DAAK N+ENIKKLSMS N + KR+RI L DC+ TTD+T+ +
Sbjct: 62  TSNLLSLKDIVELSLSVAMDAAKLNNENIKKLSMSGNIVGKRDRIALHDCVKTTDRTIYE 121

Query: 131 LDKVVEDLREYPNKKSIILYADDLKTFLSSAMTNQETCLDGFSDEEAEKRVLKLIETPHI 190
           LDK ++D  EYPNKKS+  YADDLKTFLSSA+TNQ TCLDG S ++ EKRVL+LIE  H 
Sbjct: 122 LDKAIQDFSEYPNKKSLASYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENTHN 181

Query: 191 HVTKLCSNVLALVKNLTDTDLTL---RGLVPED-PNKIASSWHAYESDPKIVLFSDLTN- 250
            VTKLCSN LALVK LT TD+ L   + LV  D P KI +S  +   DPKIVLFS+  + 
Sbjct: 182 QVTKLCSNALALVKKLT-TDVALTDEKSLVVHDFPYKI-TSISSQIDDPKIVLFSNQEDG 241

Query: 251 EEEIREGLEDGIKWPKWMSISDQRLLESSSEAAAADVVVAADGSGNFRTVSEAVAAAPNK 310
           E   RE LE+GIKWP+WMSI DQ LL+SSSE  AADVVVAADG+GN++TVSEAV AAP+K
Sbjct: 242 ESRRREDLENGIKWPEWMSIQDQMLLDSSSE-EAADVVVAADGTGNYKTVSEAVKAAPSK 301

Query: 311 SSKRYVIRIKAGVYRENVEVPSNKKNIMFWGDGRSNTFITASRSNGTGWSTFDSATVVAV 370
           +S RY+I+IKAGVYRENV+VPS+K+NIMFWGDGRSNT ITA RS+G+GWSTF+SATVVAV
Sbjct: 302 NS-RYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITADRSHGSGWSTFNSATVVAV 361

Query: 371 GDGFLARDITIENKAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQFFVNCVI 430
           GDGFLARDIT +N AG+AN QAVALRVGSDHSAFYRC + AYQDTLYVHSNRQFFV CV+
Sbjct: 362 GDGFLARDITFQNTAGSANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVKCVV 421

Query: 431 AGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRTEANQNTGIVIQKCTIKATSDL 490
           AGT+DFIFGNAA VIQ+S LTPR+P PNQ NMVTAQ RT+ NQNTGIVIQKC IKATSDL
Sbjct: 422 AGTVDFIFGNAAAVIQNSDLTPRKPGPNQNNMVTAQSRTDLNQNTGIVIQKCRIKATSDL 481

Query: 491 EPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEYDNKGDG 550
           EPVIKEFPSFLGRPW+EYARVVVMQT ITNVID +GW+ W+GD  +K  +FAEYDN G G
Sbjct: 482 EPVIKEFPSFLGRPWEEYARVVVMQTSITNVIDKEGWSTWNGD--IKKPYFAEYDNNGAG 541

Query: 551 ANTCCRVEWSSLINADQAKAFTAGQFISGADWLPSTAFPYQLGL 589
           A+T  RV WS +IN  +AK FTA  FI GA WLPST FPYQLGL
Sbjct: 542 ADTSGRVSWSLVINEAEAKTFTAEPFIDGAGWLPSTGFPYQLGL 579

BLAST of Tan0007352 vs. NCBI nr
Match: XP_004139620.2 (pectinesterase [Cucumis sativus] >KGN44621.1 hypothetical protein Csa_015743 [Cucumis sativus])

HSP 1 Score: 759.2 bits (1959), Expect = 2.6e-215
Identity = 403/584 (69.01%), Postives = 477/584 (81.68%), Query Frame = 0

Query: 11  KNELLVLALFA-TLLTNAIVVISEFNYDENSLNNVGIVEHSCATTLYPQLCHSTISSIVG 70
           +N  L LAL A  LLTNAIVV    N +ENS     ++E SCA TLYPQLCHSTISSIVG
Sbjct: 2   ENNKLSLALLAILLLTNAIVV--RANSNENS-----VIETSCAITLYPQLCHSTISSIVG 61

Query: 71  ASEFLSVKDIVEISLSVAIDAAKYNHENIKKLSMSLNDLTKRERITLRDCIVTTDKTLED 130
            S  LS+KDI E+SLSVA+DAAK+N++NIKKL +S N+++KR++I L DC+ TTD+T+ +
Sbjct: 62  TSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIYE 121

Query: 131 LDKVVEDLREYPNKKSIILYADDLKTFLSSAMTNQETCLDGFSDEEAEKRVLKLIETPHI 190
           L K +E  REYPNK+S+ LYADDLKTFLSSA+TNQ TCLDG S ++ EKRVL+LIE  HI
Sbjct: 122 LGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENAHI 181

Query: 191 HVTKLCSNVLALVKNLTDTDLTL---RGLVPED-PNKIASSWHAYESDPKIVLFSDLTNE 250
           HVTKLCSN LALV+ LT TD+ +   + LV  D P KI +S  +   DPKIVLFS+   +
Sbjct: 182 HVTKLCSNALALVQKLT-TDIAITDEKSLVVHDFPYKI-TSIPSQMDDPKIVLFSNQEED 241

Query: 251 E-EIREGLEDGIKWPKWMSISDQRLLESSSEAAAADVVVAADGSGNFRTVSEAVAAAPNK 310
           E   RE LEDGIKWPKWMSI DQ+LLESSSE AAA+ VVAADGSGN++TV+EAVAAAP+K
Sbjct: 242 ENRRREELEDGIKWPKWMSIEDQKLLESSSE-AAAEAVVAADGSGNYKTVAEAVAAAPSK 301

Query: 311 SSKRYVIRIKAGVYRENVEVPSNKKNIMFWGDGRSNTFITASRSNGTGWSTFDSATVVAV 370
           +SKRY+I+IKAG Y ENV+VPS+K+NIMFWGDGRSNT I ++RS+GTGWSTF SAT+ AV
Sbjct: 302 NSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSATLAAV 361

Query: 371 GDGFLARDITIENKAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQFFVNCVI 430
           GDGFLARDIT +NKAGAAN QAVALRVGSDHSAFYRC + AYQDTLYVHSNRQFFVNC++
Sbjct: 362 GDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIV 421

Query: 431 AGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRTEANQNTGIVIQKCTIKATSDL 490
           AGT+DFIFGNAA V Q+S +TPR+P P+Q+NMVTAQ RT+ NQNTGIVIQKC IKATSDL
Sbjct: 422 AGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDL 481

Query: 491 EPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEYDNKGDG 550
           EPVI+EFPSFLGRPW+EYARVVVMQT I+NVID +GW+ W+G    K+ ++AEYDN G G
Sbjct: 482 EPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQ--RKSPYYAEYDNNGAG 541

Query: 551 ANTCCRVEWSSLINADQAKAFTAGQFISGADWLPSTAFPYQLGL 589
           A+   RV WS +I+  QAK FTAG FI GADWL ST FPYQL L
Sbjct: 542 ADISGRVPWSLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSL 573

BLAST of Tan0007352 vs. ExPASy TrEMBL
Match: A0A1S3C861 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497582 PE=3 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 8.1e-223
Identity = 413/584 (70.72%), Postives = 479/584 (82.02%), Query Frame = 0

Query: 11  KNELLVLALFAT-LLTNAIVVISEFNYDENSLNNVGIVEHSCATTLYPQLCHSTISSIVG 70
           +N  L LALFAT LLTNAI+V +  N +    N   ++E SCA TLYPQLCHSTISSI+G
Sbjct: 2   ENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSIIG 61

Query: 71  ASEFLSVKDIVEISLSVAIDAAKYNHENIKKLSMSLNDLTKRERITLRDCIVTTDKTLED 130
            S  LS+KDIV++SLSVA+DAAK N+ NIK L MS N+++KR+RI L DC+ +TD+T+ +
Sbjct: 62  TSNLLSLKDIVQLSLSVAMDAAKLNNGNIKNL-MSFNNVSKRDRIPLHDCVKSTDRTIYE 121

Query: 131 LDKVVEDLREYPNKKSIILYADDLKTFLSSAMTNQETCLDGFSDEEAEKRVLKLIETPHI 190
           LDK +ED REYPNKKS+  YADDLKTFLSSA+TNQ TCLDGF D++ EK VL++IE  HI
Sbjct: 122 LDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKGVLRVIENTHI 181

Query: 191 HVTKLCSNVLALVKNLTDTDLTL---RGLVPED-PNKIASSWHAYESDPKIVLFSDL-TN 250
           H TKLCSN LALVK LT T++ L   + LV  D P KI +S  +  +D KIVLFS+    
Sbjct: 182 HATKLCSNALALVKKLT-TEIALIDEKNLVVHDFPYKI-TSISSQINDLKIVLFSNQEEG 241

Query: 251 EEEIREGLEDGIKWPKWMSISDQRLLESSSEAAAADVVVAADGSGNFRTVSEAVAAAPNK 310
           E   RE LE+GIKWP+WMSI DQ+LLESSS  AAADVVVA DGSGN++TVSEAV AAP+K
Sbjct: 242 ENGRREDLENGIKWPEWMSIQDQKLLESSSSEAAADVVVAVDGSGNYKTVSEAVEAAPSK 301

Query: 311 SSKRYVIRIKAGVYRENVEVPSNKKNIMFWGDGRSNTFITASRSNGTGWSTFDSATVVAV 370
           +SKRY+I+IKAGVYRENV+VPS+K+NIMFWGDGRSNT ITA RS+G+GWSTF+SATVVAV
Sbjct: 302 NSKRYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAV 361

Query: 371 GDGFLARDITIENKAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQFFVNCVI 430
           GDGFLARDI+ EN AG AN QAVALRVGSDHSAFYRCG+  YQDTLYVHSNRQFFVNCV+
Sbjct: 362 GDGFLARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVV 421

Query: 431 AGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRTEANQNTGIVIQKCTIKATSDL 490
           AGT+DF+FGNAA VIQ+S LTPR+P PNQ+NMVTAQ RT+ NQNTGIVIQKC IKATSDL
Sbjct: 422 AGTVDFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDL 481

Query: 491 EPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEYDNKGDG 550
           EPVIKEFPSFLGRPW+EYARVVVMQT ITNVID KGW+ W+GD  +K  +FAEYDN G G
Sbjct: 482 EPVIKEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYFAEYDNNGAG 541

Query: 551 ANTCCRVEWSSLINADQAKAFTAGQFISGADWLPSTAFPYQLGL 589
           ANT  RV WS +IN   AK FTA +FI GADWLPST FPYQLGL
Sbjct: 542 ANTSGRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 580

BLAST of Tan0007352 vs. ExPASy TrEMBL
Match: A0A5D3E4E8 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold343G001330 PE=3 SV=1)

HSP 1 Score: 780.8 bits (2015), Expect = 4.0e-222
Identity = 407/580 (70.17%), Postives = 472/580 (81.38%), Query Frame = 0

Query: 11  KNELLVLALFAT-LLTNAIVVISEFNYDENSLNNVGIVEHSCATTLYPQLCHSTISSIVG 70
           +N  L LALFAT LLTNAI+V +  N +    N   ++E SCA TLYPQLCHSTISSI+G
Sbjct: 2   ENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSIIG 61

Query: 71  ASEFLSVKDIVEISLSVAIDAAKYNHENIKKLSMSLNDLTKRERITLRDCIVTTDKTLED 130
            S  LS+KDIV++SLSVA+DAAK N+ NIKKL+MS+N+++KR+RI L DC+ +TD+T+ +
Sbjct: 62  TSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSVNNVSKRDRIPLHDCVKSTDRTIYE 121

Query: 131 LDKVVEDLREYPNKKSIILYADDLKTFLSSAMTNQETCLDGFSDEEAEKRVLKLIETPHI 190
           LDK +ED REYPNKKS+  YADDLKTFLSSA+TNQ TCLDGF D++ EKRVL++IE  HI
Sbjct: 122 LDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKRVLRVIENTHI 181

Query: 191 HVTKLCSNVLALVKNLTDTDLTLRGLVPEDPNKIASSWHAYESDPKIVLFSDL-TNEEEI 250
           H TKLCSN LALVK LT T++ L      D   +  S  A       VLFS+    E   
Sbjct: 182 HATKLCSNALALVKKLT-TEIAL-----IDEKNLKKSRKARSH----VLFSNQEEGENGR 241

Query: 251 REGLEDGIKWPKWMSISDQRLLESSSEAAAADVVVAADGSGNFRTVSEAVAAAPNKSSKR 310
           RE LE+GIKWP+WMSI DQ+LLESSS  AA DVVVA DGSGN++TVSEAV AAP+K+SKR
Sbjct: 242 REDLENGIKWPEWMSIQDQKLLESSSSEAAVDVVVAVDGSGNYKTVSEAVEAAPSKNSKR 301

Query: 311 YVIRIKAGVYRENVEVPSNKKNIMFWGDGRSNTFITASRSNGTGWSTFDSATVVAVGDGF 370
           Y+I+IKAGVYRENV+VPS+K+NIMFWGDGRSNT ITA RS+G+GWSTF+SATVVAVGDGF
Sbjct: 302 YIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVGDGF 361

Query: 371 LARDITIENKAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQFFVNCVIAGTI 430
           LARDI+ EN AG AN QAVALRVGSDHSAFYRCG+  YQDTLYVHSNRQFFVNCV+AGT+
Sbjct: 362 LARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVAGTV 421

Query: 431 DFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRTEANQNTGIVIQKCTIKATSDLEPVI 490
           DF+FGNAA VIQ+S LTPR+P PNQ+NMVTAQ RT+ NQNTGIVIQKC IKATSDLEPVI
Sbjct: 422 DFVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLEPVI 481

Query: 491 KEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEYDNKGDGANTC 550
           KEFPSFLGRPW+EYARVVVMQT ITNVID KGW+ W+GD  +K  +FAEYDN G GANT 
Sbjct: 482 KEFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYFAEYDNNGAGANTS 541

Query: 551 CRVEWSSLINADQAKAFTAGQFISGADWLPSTAFPYQLGL 589
            RV WS +IN   AK FTA +FI GADWLPST FPYQLGL
Sbjct: 542 GRVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 569

BLAST of Tan0007352 vs. ExPASy TrEMBL
Match: A0A5A7SNA2 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19G001370 PE=3 SV=1)

HSP 1 Score: 778.9 bits (2010), Expect = 1.5e-221
Identity = 403/579 (69.60%), Postives = 468/579 (80.83%), Query Frame = 0

Query: 11  KNELLVLALFAT-LLTNAIVVISEFNYDENSLNNVGIVEHSCATTLYPQLCHSTISSIVG 70
           +N  L LALFAT LLTNAI+V +  N +    N   ++E SCA TLYPQLCHSTISSI+G
Sbjct: 2   ENTKLSLALFATLLLTNAIIVRANSNENGVMDNISSLIETSCAITLYPQLCHSTISSIIG 61

Query: 71  ASEFLSVKDIVEISLSVAIDAAKYNHENIKKLSMSLNDLTKRERITLRDCIVTTDKTLED 130
            S  LS+KDIV++SLSVA+DAAK N+ NIKKL+MS+N+++KR+RI L DC+ +TD+T+ +
Sbjct: 62  TSNLLSLKDIVQLSLSVAMDAAKLNNGNIKKLTMSVNNVSKRDRIPLHDCVKSTDRTIYE 121

Query: 131 LDKVVEDLREYPNKKSIILYADDLKTFLSSAMTNQETCLDGFSDEEAEKRVLKLIETPHI 190
           LDK +ED REYPNKKS+  YADDLKTFLSSA+TNQ TCLDGF D++ EKRVL++IE  HI
Sbjct: 122 LDKAIEDFREYPNKKSLTSYADDLKTFLSSAITNQVTCLDGFQDDKTEKRVLRVIENTHI 181

Query: 191 HVTKLCSNVLALVKNLTDTDLTLRGLVPEDPNKIASSWHAYESDPKIVLFSDLTNEEEIR 250
           H TKLCSN LALVK LT    T   L+ E   K +    ++E             E   R
Sbjct: 182 HATKLCSNALALVKKLT----TEIALIDEKNLKKSRKARSHEE-----------GENGRR 241

Query: 251 EGLEDGIKWPKWMSISDQRLLESSSEAAAADVVVAADGSGNFRTVSEAVAAAPNKSSKRY 310
           E LE+GIKWP+WMSI DQ+LLESSS  AA DVVVA DGSGN++TVSEAV AAP+K+SKRY
Sbjct: 242 EDLENGIKWPEWMSIQDQKLLESSSSEAAVDVVVAVDGSGNYKTVSEAVEAAPSKNSKRY 301

Query: 311 VIRIKAGVYRENVEVPSNKKNIMFWGDGRSNTFITASRSNGTGWSTFDSATVVAVGDGFL 370
           +I+IKAGVYRENV+VPS+K+NIMFWGDGRSNT ITA RS+G+GWSTF+SATVVAVGDGFL
Sbjct: 302 IIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITAYRSHGSGWSTFNSATVVAVGDGFL 361

Query: 371 ARDITIENKAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQFFVNCVIAGTID 430
           ARDI+ EN AG AN QAVALRVGSDHSAFYRCG+  YQDTLYVHSNRQFFVNCV+AGT+D
Sbjct: 362 ARDISFENTAGPANGQAVALRVGSDHSAFYRCGMLGYQDTLYVHSNRQFFVNCVVAGTVD 421

Query: 431 FIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRTEANQNTGIVIQKCTIKATSDLEPVIK 490
           F+FGNAA VIQ+S LTPR+P PNQ+NMVTAQ RT+ NQNTGIVIQKC IKATSDLEPVIK
Sbjct: 422 FVFGNAAAVIQNSDLTPRKPGPNQRNMVTAQSRTDLNQNTGIVIQKCRIKATSDLEPVIK 481

Query: 491 EFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEYDNKGDGANTCC 550
           EFPSFLGRPW+EYARVVVMQT ITNVID KGW+ W+GD  +K  +FAEYDN G GANT  
Sbjct: 482 EFPSFLGRPWEEYARVVVMQTTITNVIDKKGWSTWNGD--IKKPYFAEYDNNGAGANTSG 541

Query: 551 RVEWSSLINADQAKAFTAGQFISGADWLPSTAFPYQLGL 589
           RV WS +IN   AK FTA +FI GADWLPST FPYQLGL
Sbjct: 542 RVPWSLVINEADAKTFTAERFIGGADWLPSTGFPYQLGL 563

BLAST of Tan0007352 vs. ExPASy TrEMBL
Match: A0A0A0K6S6 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G343850 PE=3 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 6.4e-220
Identity = 413/584 (70.72%), Postives = 477/584 (81.68%), Query Frame = 0

Query: 11  KNELLVLALFAT-LLTNAIVVISEFNYDENSLNNVGIVEHSCATTLYPQLCHSTISSIVG 70
           +N  L LALFAT LLTNAI+V +  N +    N + ++E SCA TLYPQLCHSTISSI+G
Sbjct: 2   ENTKLSLALFATLLLTNAIIVRANSNENGVMNNILSVIETSCAITLYPQLCHSTISSILG 61

Query: 71  ASEFLSVKDIVEISLSVAIDAAKYNHENIKKLSMSLNDLTKRERITLRDCIVTTDKTLED 130
            S  LS+KDIVE+SLSVA+DAAK N+ENIKKLSMS N + KR+RI L DC+ TTD+T+ +
Sbjct: 62  TSNLLSLKDIVELSLSVAMDAAKLNNENIKKLSMSGNIVGKRDRIALHDCVKTTDRTIYE 121

Query: 131 LDKVVEDLREYPNKKSIILYADDLKTFLSSAMTNQETCLDGFSDEEAEKRVLKLIETPHI 190
           LDK ++D  EYPNKKS+  YADDLKTFLSSA+TNQ TCLDG S ++ EKRVL+LIE  H 
Sbjct: 122 LDKAIQDFSEYPNKKSLASYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENTHN 181

Query: 191 HVTKLCSNVLALVKNLTDTDLTL---RGLVPED-PNKIASSWHAYESDPKIVLFSDLTN- 250
            VTKLCSN LALVK LT TD+ L   + LV  D P KI +S  +   DPKIVLFS+  + 
Sbjct: 182 QVTKLCSNALALVKKLT-TDVALTDEKSLVVHDFPYKI-TSISSQIDDPKIVLFSNQEDG 241

Query: 251 EEEIREGLEDGIKWPKWMSISDQRLLESSSEAAAADVVVAADGSGNFRTVSEAVAAAPNK 310
           E   RE LE+GIKWP+WMSI DQ LL+SSSE  AADVVVAADG+GN++TVSEAV AAP+K
Sbjct: 242 ESRRREDLENGIKWPEWMSIQDQMLLDSSSE-EAADVVVAADGTGNYKTVSEAVKAAPSK 301

Query: 311 SSKRYVIRIKAGVYRENVEVPSNKKNIMFWGDGRSNTFITASRSNGTGWSTFDSATVVAV 370
           +S RY+I+IKAGVYRENV+VPS+K+NIMFWGDGRSNT ITA RS+G+GWSTF+SATVVAV
Sbjct: 302 NS-RYIIKIKAGVYRENVDVPSSKRNIMFWGDGRSNTIITADRSHGSGWSTFNSATVVAV 361

Query: 371 GDGFLARDITIENKAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQFFVNCVI 430
           GDGFLARDIT +N AG+AN QAVALRVGSDHSAFYRC + AYQDTLYVHSNRQFFV CV+
Sbjct: 362 GDGFLARDITFQNTAGSANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVKCVV 421

Query: 431 AGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRTEANQNTGIVIQKCTIKATSDL 490
           AGT+DFIFGNAA VIQ+S LTPR+P PNQ NMVTAQ RT+ NQNTGIVIQKC IKATSDL
Sbjct: 422 AGTVDFIFGNAAAVIQNSDLTPRKPGPNQNNMVTAQSRTDLNQNTGIVIQKCRIKATSDL 481

Query: 491 EPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEYDNKGDG 550
           EPVIKEFPSFLGRPW+EYARVVVMQT ITNVID +GW+ W+GD  +K  +FAEYDN G G
Sbjct: 482 EPVIKEFPSFLGRPWEEYARVVVMQTSITNVIDKEGWSTWNGD--IKKPYFAEYDNNGAG 541

Query: 551 ANTCCRVEWSSLINADQAKAFTAGQFISGADWLPSTAFPYQLGL 589
           A+T  RV WS +IN  +AK FTA  FI GA WLPST FPYQLGL
Sbjct: 542 ADTSGRVSWSLVINEAEAKTFTAEPFIDGAGWLPSTGFPYQLGL 579

BLAST of Tan0007352 vs. ExPASy TrEMBL
Match: A0A0A0K9Y3 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G343870 PE=3 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 1.3e-215
Identity = 403/584 (69.01%), Postives = 477/584 (81.68%), Query Frame = 0

Query: 11  KNELLVLALFA-TLLTNAIVVISEFNYDENSLNNVGIVEHSCATTLYPQLCHSTISSIVG 70
           +N  L LAL A  LLTNAIVV    N +ENS     ++E SCA TLYPQLCHSTISSIVG
Sbjct: 2   ENNKLSLALLAILLLTNAIVV--RANSNENS-----VIETSCAITLYPQLCHSTISSIVG 61

Query: 71  ASEFLSVKDIVEISLSVAIDAAKYNHENIKKLSMSLNDLTKRERITLRDCIVTTDKTLED 130
            S  LS+KDI E+SLSVA+DAAK+N++NIKKL +S N+++KR++I L DC+ TTD+T+ +
Sbjct: 62  TSNLLSLKDIFEVSLSVAMDAAKHNNKNIKKLMVSTNNVSKRDKIGLHDCVETTDRTIYE 121

Query: 131 LDKVVEDLREYPNKKSIILYADDLKTFLSSAMTNQETCLDGFSDEEAEKRVLKLIETPHI 190
           L K +E  REYPNK+S+ LYADDLKTFLSSA+TNQ TCLDG S ++ EKRVL+LIE  HI
Sbjct: 122 LGKAIEVFREYPNKRSLTLYADDLKTFLSSAITNQVTCLDGLSHDKTEKRVLRLIENAHI 181

Query: 191 HVTKLCSNVLALVKNLTDTDLTL---RGLVPED-PNKIASSWHAYESDPKIVLFSDLTNE 250
           HVTKLCSN LALV+ LT TD+ +   + LV  D P KI +S  +   DPKIVLFS+   +
Sbjct: 182 HVTKLCSNALALVQKLT-TDIAITDEKSLVVHDFPYKI-TSIPSQMDDPKIVLFSNQEED 241

Query: 251 E-EIREGLEDGIKWPKWMSISDQRLLESSSEAAAADVVVAADGSGNFRTVSEAVAAAPNK 310
           E   RE LEDGIKWPKWMSI DQ+LLESSSE AAA+ VVAADGSGN++TV+EAVAAAP+K
Sbjct: 242 ENRRREELEDGIKWPKWMSIEDQKLLESSSE-AAAEAVVAADGSGNYKTVAEAVAAAPSK 301

Query: 311 SSKRYVIRIKAGVYRENVEVPSNKKNIMFWGDGRSNTFITASRSNGTGWSTFDSATVVAV 370
           +SKRY+I+IKAG Y ENV+VPS+K+NIMFWGDGRSNT I ++RS+GTGWSTF SAT+ AV
Sbjct: 302 NSKRYIIKIKAGEYWENVDVPSSKRNIMFWGDGRSNTKIISNRSHGTGWSTFKSATLAAV 361

Query: 371 GDGFLARDITIENKAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQFFVNCVI 430
           GDGFLARDIT +NKAGAAN QAVALRVGSDHSAFYRC + AYQDTLYVHSNRQFFVNC++
Sbjct: 362 GDGFLARDITFQNKAGAANGQAVALRVGSDHSAFYRCSMLAYQDTLYVHSNRQFFVNCIV 421

Query: 431 AGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRTEANQNTGIVIQKCTIKATSDL 490
           AGT+DFIFGNAA V Q+S +TPR+P P+Q+NMVTAQ RT+ NQNTGIVIQKC IKATSDL
Sbjct: 422 AGTVDFIFGNAAAVFQNSDITPRKPGPSQRNMVTAQSRTDINQNTGIVIQKCRIKATSDL 481

Query: 491 EPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEYDNKGDG 550
           EPVI+EFPSFLGRPW+EYARVVVMQT I+NVID +GW+ W+G    K+ ++AEYDN G G
Sbjct: 482 EPVIEEFPSFLGRPWEEYARVVVMQTTISNVIDKEGWSTWNGQ--RKSPYYAEYDNNGAG 541

Query: 551 ANTCCRVEWSSLINADQAKAFTAGQFISGADWLPSTAFPYQLGL 589
           A+   RV WS +I+  QAK FTAG FI GADWL ST FPYQL L
Sbjct: 542 ADISGRVPWSLVIDEAQAKTFTAGPFIGGADWLSSTGFPYQLSL 573

BLAST of Tan0007352 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 570.5 bits (1469), Expect = 1.6e-162
Identity = 314/590 (53.22%), Postives = 400/590 (67.80%), Query Frame = 0

Query: 10  KKNELLVL--ALFATLLTNAIVVISEFNYDEN-----SLNNVGIVEHSCATTLYPQLCHS 69
           KKN+ LVL  A  A L   A+  IS      N     S ++  ++  SC++T YP+LC S
Sbjct: 15  KKNKKLVLLSAAVALLFVAAVAGISAGASKANEKRTLSPSSHAVLRSSCSSTRYPELCIS 74

Query: 70  TISSIVGASEFLSVKDIVEISLSVAIDAAKYNHENIKKLSMSLNDLTKRERITLRDCIVT 129
            + +  G  E  S KD++E S+++ I A ++N+  +KKL      LT RE+  L DC+ T
Sbjct: 75  AVVT-AGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKRKGLTPREKTALHDCLET 134

Query: 130 TDKTLEDLDKVVEDLREYPNKKSIILYADDLKTFLSSAMTNQETCLDGFSDEEAEKRVLK 189
            D+TL++L + VEDL  YP KK++  +A DLKT +SSA+TNQETCLDGFS ++A+K+V K
Sbjct: 135 IDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQETCLDGFSHDDADKQVRK 194

Query: 190 LIETPHIHVTKLCSNVLALVKNLTDTDLTLRGLVPEDPNKIASSWHAY-ESDPKIVLFSD 249
            +    IHV  +CSN LA++KN+TDTD+       E   KI S+     E + +  +  D
Sbjct: 195 ALLKGQIHVEHMCSNALAMIKNMTDTDIANF----EQKAKITSNNRKLKEENQETTVAVD 254

Query: 250 LTNEEEIREGLEDGIKWPKWMSISDQRLLESSSEAAAADVVVAADGSGNFRTVSEAVAAA 309
           +    E+     D   WP W+S  D+RLL+ S     AD  VAADGSG F+TV+ AVAAA
Sbjct: 255 IAGAGEL-----DSEGWPTWLSAGDRRLLQGS--GVKADATVAADGSGTFKTVAAAVAAA 314

Query: 310 PNKSSKRYVIRIKAGVYRENVEVPSNKKNIMFWGDGRSNTFITASRSNGTGWSTFDSATV 369
           P  S+KRYVI IKAGVYRENVEV   KKNIMF GDGR+ T IT SR+   G +TF SATV
Sbjct: 315 PENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDGSTTFHSATV 374

Query: 370 VAVGDGFLARDITIENKAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQFFVN 429
            AVG+ FLARDIT +N AG + HQAVALRVGSD SAFY C + AYQDTLYVHSNRQFFV 
Sbjct: 375 AAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYVHSNRQFFVK 434

Query: 430 CVIAGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRTEANQNTGIVIQKCTIKAT 489
           C+IAGT+DFIFGNAAVV+QD  +  RRP+  Q+NMVTAQGRT+ NQNTGIVIQKC I AT
Sbjct: 435 CLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIVIQKCRIGAT 494

Query: 490 SDLEPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEYDNK 549
           SDL+ V   FP++LGRPWKEY++ V+MQ+ I++VI P+GW+ W+G FAL TL + EY N 
Sbjct: 495 SDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNTLTYREYSNT 554

Query: 550 GDGANTCCRVEWSS---LINADQAKAFTAGQFISGADWLPSTAFPYQLGL 589
           G GA T  RV+W     +  A +A+ +TAGQFI G  WL ST FP+ LGL
Sbjct: 555 GAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

BLAST of Tan0007352 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 557.0 bits (1434), Expect = 1.8e-158
Identity = 310/600 (51.67%), Postives = 402/600 (67.00%), Query Frame = 0

Query: 4   TKVSLKKKNELLVL--ALFATLLTNAIVVISEFNYDEN--------SLNNVGIVEHSCAT 63
           +K S  K N+ L+L  A  A LL  +IV I+    ++N        S  +  I++  C++
Sbjct: 9   SKFSDFKNNKKLILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSS 68

Query: 64  TLYPQLCHSTISSIVGASEFLSVKDIVEISLSVAIDAAKYNHENIKKLSMSLNDLTKRER 123
           TLYP+LC S +++  G  E  S K+++E SL++   A K+N+  +KKL      LT RE 
Sbjct: 69  TLYPELCFSAVAA-TGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREV 128

Query: 124 ITLRDCIVTTDKTLEDLDKVVEDLREYPNKKSIILYADDLKTFLSSAMTNQETCLDGFSD 183
             L DC+ T D+TL++L   VEDL +YP +KS+  +ADDLKT +SSA+TNQ TCLDGFS 
Sbjct: 129 TALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSY 188

Query: 184 EEAEKRVLKLIETPHIHVTKLCSNVLALVKNLTDTDLTLRGLVPEDPNKIASSWHAYESD 243
           ++A+++V K +    +HV  +CSN LA++KN+T+TD+                   +E  
Sbjct: 189 DDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIA-----------------NFELR 248

Query: 244 PKIVLFSDLTNEEEIRE--GLEDGIKWPKWMSISDQRLLESSSEAAAADVVVAADGSGNF 303
            K   F++  N  +++E  G  D   WPKW+S+ D+RLL+ S+    AD  VA DGSG+F
Sbjct: 249 DKSSTFTN-NNNRKLKEVTGDLDSDGWPKWLSVGDRRLLQGST--IKADATVADDGSGDF 308

Query: 304 RTVSEAVAAAPNKSSKRYVIRIKAGVYRENVEVPSNKKNIMFWGDGRSNTFITASRSNGT 363
            TV+ AVAAAP KS+KR+VI IKAGVYRENVEV   K NIMF GDGR  T IT SR+   
Sbjct: 309 TTVAAAVAAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVD 368

Query: 364 GWSTFDSATVVAVGDGFLARDITIENKAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLY 423
           G +TF SATV AVG+ FLARDIT +N AG + HQAVALRVGSD SAFY+C + AYQDTLY
Sbjct: 369 GSTTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLY 428

Query: 424 VHSNRQFFVNCVIAGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRTEANQNTGI 483
           VHSNRQFFV C I GT+DFIFGNAA V+QD  +  RRP+  Q+NMVTAQGR++ NQNTGI
Sbjct: 429 VHSNRQFFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGI 488

Query: 484 VIQKCTIKATSDLEPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALK 543
           VIQ C I  TSDL  V   FP++LGRPWKEY+R V+MQ+DI++VI P+GW  WSG FAL 
Sbjct: 489 VIQNCRIGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALD 548

Query: 544 TLFFAEYDNKGDGANTCCRVEWS--SLINAD-QAKAFTAGQFISGADWLPSTAFPYQLGL 589
           TL + EY N+G GA T  RV+W    +I +D +A+ FTAGQFI G  WL ST FP+ L L
Sbjct: 549 TLTYREYLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

BLAST of Tan0007352 vs. TAIR 10
Match: AT4G02300.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 372.1 bits (954), Expect = 8.3e-103
Identity = 213/521 (40.88%), Postives = 295/521 (56.62%), Query Frame = 0

Query: 71  SEFLSVKDIVEISLSVAIDAAKYNHENIKKLSMSL-NDLTKRERITLRDCIVTTDKTLED 130
           +E  ++ +++   L++ I        N   L   L  +LT  ER    DC+   D T+ D
Sbjct: 48  TETKTIPELIIADLNLTILKVNLASSNFSDLQTRLFPNLTHYERCAFEDCLGLLDDTISD 107

Query: 131 LDKVVEDLREYPNKKSIILYADDLKTFLSSAMTNQETCLDGFSDEEAEKRVLKLIETPHI 190
           L+  V DLR      S  L  +D+   L++ MT Q+TCLDGFS  + E       E P  
Sbjct: 108 LETAVSDLR------SSSLEFNDISMLLTNVMTYQDTCLDGFSTSDNENNNDMTYELPE- 167

Query: 191 HVTKLCSNVLALVKNLTDTDLTLRGLVPEDPNKIASSWHAYESDPKIVLFSDLTNEEEIR 250
                                 L+ ++ +  N +++S H         +   ++ ++   
Sbjct: 168 ---------------------NLKEIILDISNNLSNSLH---------MLQVISRKKPSP 227

Query: 251 EGLEDGIKWPKWMSISDQRLLESSSEAAAADVVVAADGSGNFRTVSEAVAAAPNKSSKRY 310
           +  E  +++P W+S +DQRLLE+  +    ++ VA DG+GNF T+++AV AAPN S  R+
Sbjct: 228 KSSEVDVEYPSWLSENDQRLLEAPVQETNYNLSVAIDGTGNFTTINDAVFAAPNMSETRF 287

Query: 311 VIRIKAGVYRENVEVPSNKKNIMFWGDGRSNTFITASRSNGTGWSTFDSATVVAVGDGFL 370
           +I IK G Y ENVE+P  K  IMF GDG   T I A+RS   GWSTF + TV   G G++
Sbjct: 288 IIYIKGGEYFENVELPKKKTMIMFIGDGIGKTVIKANRSRIDGWSTFQTPTVGVKGKGYI 347

Query: 371 ARDITIENKAGAANHQAVALRVGSDHSAFYRCGIQAYQDTLYVHSNRQFFVNCVIAGTID 430
           A+DI+  N AG A  QAVA R GSDHSAFYRC    YQDTLYVHS +QF+  C I GTID
Sbjct: 348 AKDISFVNSAGPAKAQAVAFRSGSDHSAFYRCEFDGYQDTLYVHSAKQFYRECDIYGTID 407

Query: 431 FIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRTEANQNTGIVIQKCTIKATSDLEPVIK 490
           FIFGNAAVV Q+S L  R+P+P  +   TAQ R +++Q TGI I  C I A  DL PV +
Sbjct: 408 FIFGNAAVVFQNSSLYARKPNPGHKIAFTAQSRNQSDQPTGISILNCRILAAPDLIPVKE 467

Query: 491 EFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFALKTLFFAEYDNKGDGANTCC 550
            F ++LGRPW++Y+R V++++ I ++I P GW     DFAL+TL++ EY N+G GAN   
Sbjct: 468 NFKAYLGRPWRKYSRTVIIKSFIDDLIHPAGWLEGKKDFALETLYYGEYMNEGPGANMAK 527

Query: 551 RVEWSS---LINADQAKAFTAGQFISGADWLPSTAFPYQLG 588
           RV W     + N  +A  FT G FI G+ WL ST  P+ LG
Sbjct: 528 RVTWPGFRRIENQTEATQFTVGPFIDGSTWLNSTGIPFSLG 531

BLAST of Tan0007352 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 366.3 bits (939), Expect = 4.6e-101
Identity = 223/541 (41.22%), Postives = 311/541 (57.49%), Query Frame = 0

Query: 51  CATTLYPQLCHSTISSIVGASEFLSVKDIVEISLSVAIDAAKYNHENIKKLSMSLNDLTK 110
           C+ T  P+ C   ++         S  + ++IS+ + +D A     +   L     D   
Sbjct: 34  CSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISMKLVLDRAILAKTHAFTLGPKCRD--T 93

Query: 111 RERITLRDCIVTTDKTLEDLDKVVEDLREYPNKKSIILYADDLKTFLSSAMTNQETCLDG 170
           RE+    DCI   D T+  +++ ++     PN K   L   D +T+LS+A+TN +TC  G
Sbjct: 94  REKAAWEDCIKLYDLTVSKINETMD-----PNVKCSKL---DAQTWLSTALTNLDTCRAG 153

Query: 171 FSDEEAEKRVLKLIETPHIHVTKLCSNVLALVKNLTDTDLTLRGLVPEDPNKIASSWHAY 230
           F +      VL L+     +V+ L  N LA+                   NK+  ++   
Sbjct: 154 FLELGVTDIVLPLMSN---NVSNLLCNTLAI-------------------NKVPFNYTPP 213

Query: 231 ESDPKIVLFSDLTNEEEIREGLEDGIKWPKWMSISDQRLLESSSEAAAADVVVAADGSGN 290
           E D                        +P W+   D++LL+SS+    A  VVA DGSGN
Sbjct: 214 EKD-----------------------GFPSWVKPGDRKLLQSSTPKDNA--VVAKDGSGN 273

Query: 291 FRTVSEAVAAAPNKSSKRYVIRIKAGVYRENVEVPSNKKNIMFWGDGRSNTFITASRSNG 350
           F+T+ EA+ AA    S R+VI +K GVY EN+E+   KKN+M  GDG   T IT S+S G
Sbjct: 274 FKTIKEAIDAA--SGSGRFVIYVKQGVYSENLEI--RKKNVMLRGDGIGKTIITGSKSVG 333

Query: 351 TGWSTFDSATVVAVGDGFLARDITIENKAGAANHQAVALRVGSDHSAFYRCGIQAYQDTL 410
            G +TF+SATV AVGDGF+AR IT  N AGA+N QAVALR GSD S FY+C  +AYQDTL
Sbjct: 334 GGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTL 393

Query: 411 YVHSNRQFFVNCVIAGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRTEANQNTG 470
           YVHSNRQF+ +C + GT+DFIFGNAA V+Q+  +  RRP  ++ N +TAQGR++ NQNTG
Sbjct: 394 YVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRP-RSKTNTITAQGRSDPNQNTG 453

Query: 471 IVIQKCTIKATSDLEPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWSGDFAL 530
           I+I    + A SDL PV+    ++LGRPW++Y+R V M+T + ++IDP+GW  W G+FAL
Sbjct: 454 IIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFAL 511

Query: 531 KTLFFAEYDNKGDGANTCCRVEWSS---LINADQAKAFTAGQFISGADWLPSTAFPYQLG 589
           KTLF+AE+ N G GA+T  RV W     L +A +A  FT G F++G  W+PS+  P+  G
Sbjct: 514 KTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIPSSV-PFTSG 511

BLAST of Tan0007352 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 366.3 bits (939), Expect = 4.6e-101
Identity = 210/546 (38.46%), Postives = 305/546 (55.86%), Query Frame = 0

Query: 47  VEHSCATTLYPQLCHSTISSIVGASEFLSVKDIVEISLSVAIDAAKYNHENIKKLSMSLN 106
           +  +C+ +LYP LC  T+    G S      +++ IS +  +   K++       +++  
Sbjct: 75  ISRTCSKSLYPNLCIDTLLDFPG-SLTADENELIHISFNATLQ--KFSKALYTSSTITYT 134

Query: 107 DLTKRERITLRDCIVTTDKTLEDLDKVVEDLREYPNKKSIILYADDLKTFLSSAMTNQET 166
            +  R R     C+   D +++ L + +  +      +S      D+ T+LSSAMTN +T
Sbjct: 135 QMPPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDES----HSDVMTWLSSAMTNHDT 194

Query: 167 CLDGFSDEEAEKRVLKLIETPHIHVTKLCSNVLALVKNLTDTDLTLRGLVPEDPNKIASS 226
           C DGF + E +   +K               V+  VK+L++               +++ 
Sbjct: 195 CTDGFDEIEGQGGEVK-------------DQVIGAVKDLSE--------------MVSNC 254

Query: 227 WHAYESDPKIVLFSDLTNEEEIREGLEDGIKWPKWMSISDQRLLESSSEAAAADVVVAAD 286
              +    K +    + N  ++  G E+  + P W+   D+ LL + + A  AD+ V+ D
Sbjct: 255 LAIFAGKVKDLSGVPVVNNRKLL-GTEETEELPNWLKREDRELLGTPTSAIQADITVSKD 314

Query: 287 GSGNFRTVSEAVAAAPNKSSKRYVIRIKAGVY-RENVEVPSNKKNIMFWGDGRSNTFITA 346
           GSG F+T++EA+  AP  SS+R+VI +KAG Y  EN++V   K N+MF GDG+  T IT 
Sbjct: 315 GSGTFKTIAEAIKKAPEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITG 374

Query: 347 SRSNGTGWSTFDSATVVAVGDGFLARDITIENKAGAANHQAVALRVGSDHSAFYRCGIQA 406
            +S     +TF +AT  A G GF+ RD+T EN AG A HQAVALRVG DH+  YRC I  
Sbjct: 375 GKSIADDLTTFHTATFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIG 434

Query: 407 YQDTLYVHSNRQFFVNCVIAGTIDFIFGNAAVVIQDSVLTPRRPDPNQQNMVTAQGRTEA 466
           YQD LYVHSNRQFF  C I GT+DFIFGNAAV++Q   +  R+P   Q+  +TAQ R + 
Sbjct: 435 YQDALYVHSNRQFFRECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDP 494

Query: 467 NQNTGIVIQKCTIKATSDLEPVIKEFPSFLGRPWKEYARVVVMQTDITNVIDPKGWAPWS 526
           NQNTGI I  C + AT DLE     +P++LGRPWK Y+RVV M +D+ + IDP+GW  W+
Sbjct: 495 NQNTGISIHACKLLATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWN 554

Query: 527 GDFALKTLFFAEYDNKGDGANTCCRVEWSS---LINADQAKAFTAGQFISGADWLPSTAF 586
           G FAL +L++ EY NKG G+    RV+W     + +  +A  FT  QFISG+ WLPST  
Sbjct: 555 GPFALDSLYYGEYMNKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGV 585

Query: 587 PYQLGL 589
            +  GL
Sbjct: 615 SFFSGL 585

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P839486.7e-16653.12Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1[more]
O048861.2e-16552.95Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1[more]
Q431431.8e-16352.35Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PM... [more]
O490062.2e-16153.22Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME... [more]
Q425342.6e-15751.67Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
XP_008458040.21.7e-22270.72PREDICTED: LOW QUALITY PROTEIN: pectinesterase-like [Cucumis melo][more]
TYK30782.18.3e-22270.17pectinesterase-like [Cucumis melo var. makuwa][more]
KAA0026177.13.2e-22169.60pectinesterase-like [Cucumis melo var. makuwa][more]
XP_011659194.11.3e-21970.72pectinesterase [Cucumis sativus] >KGN44619.1 hypothetical protein Csa_015815 [Cu... [more]
XP_004139620.22.6e-21569.01pectinesterase [Cucumis sativus] >KGN44621.1 hypothetical protein Csa_015743 [Cu... [more]
Match NameE-valueIdentityDescription
A0A1S3C8618.1e-22370.72Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497582 PE=3 SV=1[more]
A0A5D3E4E84.0e-22270.17Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold343G00133... [more]
A0A5A7SNA21.5e-22169.60Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold19G001370... [more]
A0A0A0K6S66.4e-22070.72Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G343850 PE=3 SV=1[more]
A0A0A0K9Y31.3e-21569.01Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_7G343870 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G14310.11.6e-16253.22pectin methylesterase 3 [more]
AT1G53830.11.8e-15851.67pectin methylesterase 2 [more]
AT4G02300.18.3e-10340.88Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT2G45220.14.6e-10141.22Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G53370.14.6e-10138.46pectin methylesterase PCR fragment F [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 42..201
e-value: 2.8E-27
score: 106.6
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 17..206
e-value: 3.0E-20
score: 70.3
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 47..201
e-value: 8.1E-25
score: 87.9
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 45..207
e-value: 9.9E-30
score: 105.5
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 46..206
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 280..573
e-value: 4.1E-133
score: 442.7
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 274..588
e-value: 9.0E-120
score: 401.8
NoneNo IPR availablePANTHERPTHR31707:SF180PECTINESTERASEcoord: 15..587
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 15..587
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 50..206
e-value: 1.63393E-35
score: 128.71
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 424..433
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 305..324
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 275..586

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0007352.1Tan0007352.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016020 membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity