Tan0007231 (gene) Snake gourd v1

Overview
NameTan0007231
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Description(1->3)-beta-glucan endohydrolase
LocationLG08: 1681580 .. 1683837 (+)
RNA-Seq ExpressionTan0007231
SyntenyTan0007231
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCAAAATCTGGATTACAAATCCACAGAACTTGCATATTCAGAACATTTCTTTTGTTGTTCTTGTCGATTTTCCCTAATGGGGAAAGTGTTGGAGTGAACTGGGGAACTATGGCCACTCACCAGCTCCCACCAGAGAAAGTTGTTAAAATGCTTCAAGAAAATGGGTTCCATAAATTGAAGCTTTTCGAAGCTGAGGATAGGATTTTGGAAGCACTTATTGGGACTGATATTGAAGTTATGTTGGCAATTCCCAATTCAATGTTGCAAATTATGAGTGAAGATTCTGAGGCTGCTGCTTCTTGGGTCGATGCTAATGTTACTGCTTATACTTATCATGGTGGTGTCAATATCAAGTTGAGTATTGAAGATTCTTGATCTCTCATTATCAAACATTTCTTTTCTTTTTTGTGTTCTTTAGGTGTTTGATGAATTGCCCACTTCATGGTTTCCTCTGTTTTTGAATATGTTCTTTTGAAGCTGCTGCTGCTGCTGATGATTTCATTTTAGATCAATTCATGGAATACCAATCTGTTTCTTTCTTCCATTTTCTTTGTTTAGTTCTATATTTGTGCTGACTGACTTTTAGAGTACTACTTTTAGCTGAAGTCCATTTTCGATAGATGTTTTTGAGAAAAGCACTAATTAGATGTTTACTCTAAAAGAGGTAACTAAATGATGTTTTTCGTTAGCTAGAAGGACTTTTCACTCATCTAAAAGTCATGTCCAACTCGCTCTTAGCTTGACTATGTAACTAAACATGAATTTTTTTAGGAAGTATACTTCTAGTTAGCTAAAAATAGGCTTTATTCATTTAAAAGGTTTTTGTCGAAGTTAGTTTGATTTATCACTTCATGAAGAATGAATCATTATCTGACCATTTGATTGTTGGTATCAAATGCAAGTAATTTCATATCTTTTATTTTGACAATGAGATTCCAAAACCATGAGAAAATAACAAAATGTGACTCTTTAATGAAGCTATAACCTTCTAGGCTGAAAGAGTTGAAATTTGTTAATGCAAATTTGGATGTTCAAAATACTAGATTTTATGAATGTTAAGTTAACTTAAAGAACATATTGATTCTTTCTCTCTCCCATAAAATTCCTTTGAAAACTTATTTTTCAGGTATGTTGCTGTAGGCAATGAGCCCTTTCTTAAGTCCTACAATGGTACTTATCTTCAGTTGACATTGCCTGCCTTGAGGAATATCCAACAAGCCTTAAATGATGCAGGGCTTGGGTCAAAGGTCAAAGCTACTATTCCTTTCAATGCTGATATTTACAACTCCCCGGACTCAAACCCGGTTCCATCTGCAGGCGACTTTCGACCCGACGTCCGAGACTTGACGATCGAGATTCTACACTTCCTATACCTAAACAATGCACCTTTCACAGTCAATATCTACCCCTTCCTTTCCCTCTATGGCAATGACTACTTCCCCATGGACTTTGCATTCTTTGATGGAACATACAAGCCCATCAAAGACGGAGATCTAACCTACACCAACGTGTTCGACGCCAATTTCGACACACTCGTTTCAGCTCTAGTGAAAGCGGGATACCCCGACATGAAGATCATAGTAGGGGAAGTCGGGTGGCCGACGGACGGCGACAAACACGCAAATATCCAAAACGCAAAGCGATTCAATCAGGGATTACTGAGACACGCTCTAAGCGGGGAGGGAACCCCAGCTCGAGTCGGCATAATTGACATCTATCTCTTCAGCCTGATTGATGAAAATGCCAAAAGCATAGAACCAGGGAGCTTTGAGAGACATTGGGGAATATTTGAGTTTGATGGTAAACCAAAATATGAACTAGATCTCTCTGGAACAGAAGAAGAAAAAGGCTTAATACCAGTAGAAGGAGTGAAATATATGGTTAAAAGATGGTGTATTTTGAATCCAAATGTGAATGATTGGGAAGGATTGGCTGATAGTGTAGATTATGCTTGTAGCTTATCAGATTGCACTGCATTGGAATATGGATCGTCTTGCAATCAATTAACTGCTCAAGGAAATGCTTCTTATGCCTTTAATATGTACTATCAAGTGAACAGCCAAAAAAGTTGGAACTGTGATTTTTCTGGTTTGGCTATGGTGACTGAAGAAGATCCTTCTTATGGGAACTGCCAGTTCCCAATTATGATTGATTATACTTCTTCTTCTTCTTCTCCATTTCTGCTTGATGAAAGATTTTTAGATCTTCTGGTTGCAATCTTCATGGGATTGTTGGTGTTTTTGTTTTTGGAATGA

mRNA sequence

ATGTCAAAATCTGGATTACAAATCCACAGAACTTGCATATTCAGAACATTTCTTTTGTTGTTCTTGTCGATTTTCCCTAATGGGGAAAGTGTTGGAGTGAACTGGGGAACTATGGCCACTCACCAGCTCCCACCAGAGAAAGTTGTTAAAATGCTTCAAGAAAATGGGTTCCATAAATTGAAGCTTTTCGAAGCTGAGGATAGGATTTTGGAAGCACTTATTGGGACTGATATTGAAGTTATGTTGGCAATTCCCAATTCAATGTTGCAAATTATGAGTGAAGATTCTGAGGCTGCTGCTTCTTGGGTCGATGCTAATGTTACTGCTTATACTTATCATGGTGGTGTCAATATCAAGTATGTTGCTGTAGGCAATGAGCCCTTTCTTAAGTCCTACAATGGTACTTATCTTCAGTTGACATTGCCTGCCTTGAGGAATATCCAACAAGCCTTAAATGATGCAGGGCTTGGGTCAAAGGTCAAAGCTACTATTCCTTTCAATGCTGATATTTACAACTCCCCGGACTCAAACCCGGTTCCATCTGCAGGCGACTTTCGACCCGACGTCCGAGACTTGACGATCGAGATTCTACACTTCCTATACCTAAACAATGCACCTTTCACAGTCAATATCTACCCCTTCCTTTCCCTCTATGGCAATGACTACTTCCCCATGGACTTTGCATTCTTTGATGGAACATACAAGCCCATCAAAGACGGAGATCTAACCTACACCAACGTGTTCGACGCCAATTTCGACACACTCGTTTCAGCTCTAGTGAAAGCGGGATACCCCGACATGAAGATCATAGTAGGGGAAGTCGGGTGGCCGACGGACGGCGACAAACACGCAAATATCCAAAACGCAAAGCGATTCAATCAGGGATTACTGAGACACGCTCTAAGCGGGGAGGGAACCCCAGCTCGAGTCGGCATAATTGACATCTATCTCTTCAGCCTGATTGATGAAAATGCCAAAAGCATAGAACCAGGGAGCTTTGAGAGACATTGGGGAATATTTGAGTTTGATGGTAAACCAAAATATGAACTAGATCTCTCTGGAACAGAAGAAGAAAAAGGCTTAATACCAGTAGAAGGAGTGAAATATATGGTTAAAAGATGGTGTATTTTGAATCCAAATGTGAATGATTGGGAAGGATTGGCTGATAGTGTAGATTATGCTTGTAGCTTATCAGATTGCACTGCATTGGAATATGGATCGTCTTGCAATCAATTAACTGCTCAAGGAAATGCTTCTTATGCCTTTAATATGTACTATCAAGTGAACAGCCAAAAAAGTTGGAACTGTGATTTTTCTGGTTTGGCTATGGTGACTGAAGAAGATCCTTCTTATGGGAACTGCCAGTTCCCAATTATGATTGATTATACTTCTTCTTCTTCTTCTCCATTTCTGCTTGATGAAAGATTTTTAGATCTTCTGGTTGCAATCTTCATGGGATTGTTGGTGTTTTTGTTTTTGGAATGA

Coding sequence (CDS)

ATGTCAAAATCTGGATTACAAATCCACAGAACTTGCATATTCAGAACATTTCTTTTGTTGTTCTTGTCGATTTTCCCTAATGGGGAAAGTGTTGGAGTGAACTGGGGAACTATGGCCACTCACCAGCTCCCACCAGAGAAAGTTGTTAAAATGCTTCAAGAAAATGGGTTCCATAAATTGAAGCTTTTCGAAGCTGAGGATAGGATTTTGGAAGCACTTATTGGGACTGATATTGAAGTTATGTTGGCAATTCCCAATTCAATGTTGCAAATTATGAGTGAAGATTCTGAGGCTGCTGCTTCTTGGGTCGATGCTAATGTTACTGCTTATACTTATCATGGTGGTGTCAATATCAAGTATGTTGCTGTAGGCAATGAGCCCTTTCTTAAGTCCTACAATGGTACTTATCTTCAGTTGACATTGCCTGCCTTGAGGAATATCCAACAAGCCTTAAATGATGCAGGGCTTGGGTCAAAGGTCAAAGCTACTATTCCTTTCAATGCTGATATTTACAACTCCCCGGACTCAAACCCGGTTCCATCTGCAGGCGACTTTCGACCCGACGTCCGAGACTTGACGATCGAGATTCTACACTTCCTATACCTAAACAATGCACCTTTCACAGTCAATATCTACCCCTTCCTTTCCCTCTATGGCAATGACTACTTCCCCATGGACTTTGCATTCTTTGATGGAACATACAAGCCCATCAAAGACGGAGATCTAACCTACACCAACGTGTTCGACGCCAATTTCGACACACTCGTTTCAGCTCTAGTGAAAGCGGGATACCCCGACATGAAGATCATAGTAGGGGAAGTCGGGTGGCCGACGGACGGCGACAAACACGCAAATATCCAAAACGCAAAGCGATTCAATCAGGGATTACTGAGACACGCTCTAAGCGGGGAGGGAACCCCAGCTCGAGTCGGCATAATTGACATCTATCTCTTCAGCCTGATTGATGAAAATGCCAAAAGCATAGAACCAGGGAGCTTTGAGAGACATTGGGGAATATTTGAGTTTGATGGTAAACCAAAATATGAACTAGATCTCTCTGGAACAGAAGAAGAAAAAGGCTTAATACCAGTAGAAGGAGTGAAATATATGGTTAAAAGATGGTGTATTTTGAATCCAAATGTGAATGATTGGGAAGGATTGGCTGATAGTGTAGATTATGCTTGTAGCTTATCAGATTGCACTGCATTGGAATATGGATCGTCTTGCAATCAATTAACTGCTCAAGGAAATGCTTCTTATGCCTTTAATATGTACTATCAAGTGAACAGCCAAAAAAGTTGGAACTGTGATTTTTCTGGTTTGGCTATGGTGACTGAAGAAGATCCTTCTTATGGGAACTGCCAGTTCCCAATTATGATTGATTATACTTCTTCTTCTTCTTCTCCATTTCTGCTTGATGAAAGATTTTTAGATCTTCTGGTTGCAATCTTCATGGGATTGTTGGTGTTTTTGTTTTTGGAATGA

Protein sequence

MSKSGLQIHRTCIFRTFLLLFLSIFPNGESVGVNWGTMATHQLPPEKVVKMLQENGFHKLKLFEAEDRILEALIGTDIEVMLAIPNSMLQIMSEDSEAAASWVDANVTAYTYHGGVNIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRPDVRDLTIEILHFLYLNNAPFTVNIYPFLSLYGNDYFPMDFAFFDGTYKPIKDGDLTYTNVFDANFDTLVSALVKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLIPVEGVKYMVKRWCILNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASYAFNMYYQVNSQKSWNCDFSGLAMVTEEDPSYGNCQFPIMIDYTSSSSSPFLLDERFLDLLVAIFMGLLVFLFLE
Homology
BLAST of Tan0007231 vs. ExPASy Swiss-Prot
Match: Q6NKW9 (Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 PE=2 SV=2)

HSP 1 Score: 547.0 bits (1408), Expect = 2.2e-154
Identity = 269/464 (57.97%), Postives = 339/464 (73.06%), Query Frame = 0

Query: 22  LSIFPNGESVGVNWGTMATHQLPPEKVVKMLQENGFHKLKLFEAEDRILEALIGTDIEVM 81
           L I  NG  +GVNWGTMATH+LPP+ VV+ML++N  +K+KLF+A++  + AL G+ +EVM
Sbjct: 18  LGILVNG--LGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGSGLEVM 77

Query: 82  LAIPNSMLQIMSEDSEAAASWVDANVTAYTYHGGVNIKYVAVGNEPFLKSYNGTYLQLTL 141
           +AIPN  L++M+   + A  WV  NVT Y + GGVNI +VAVGNEPFLKSYNG+++ LT 
Sbjct: 78  VAIPNDQLKVMT-SYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTF 137

Query: 142 PALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRPDVRDLTIEILHFLY 201
           PAL NIQ ALN+AGLG+ VKAT+P NAD+Y+SP SNPVPSAG FRPD+     +I+ FL 
Sbjct: 138 PALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLG 197

Query: 202 LNNAPFTVNIYPFLSLYGNDYFPMDFAFFDGTYKPIKDGDLTYTNVFDANFDTLVSALVK 261
            NNAP T+NIYPFLSLYGND FP+++AFFDG  +PI D  + YTNVFDANFDTLVS+L  
Sbjct: 198 KNNAPITINIYPFLSLYGNDDFPLNYAFFDGA-EPINDNGIDYTNVFDANFDTLVSSLKA 257

Query: 262 AGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVGIIDIYLFSLI 321
            G+ DM IIVGEVGWPT+GDKHAN  +A RF  GLL    + +GTP R   I++YLF L+
Sbjct: 258 VGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEVYLFGLL 317

Query: 322 DENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLIPVEGVKYMVKRWCILNPNV 381
           DE+AKSI PG FERHWGIF+FDG+PK+ +DLSG  + K LI  + V Y+  +WC  NP  
Sbjct: 318 DEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEA 377

Query: 382 NDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASYAFNMYYQVNSQKSWNCDFSGL 441
            D   LA ++DYAC+ SDCTAL YGSSCN L A GNASYAFNM++QV +Q    C F GL
Sbjct: 378 KDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESACYFQGL 437

Query: 442 AMVTEEDPSYGNCQFPIMIDYTSSSSSPFLLDERFLDLLVAIFM 486
           A +T ++ S G C FPI I   +SS+S F      L +L+  F+
Sbjct: 438 ATITTQNISQGQCNFPIQI--VASSASSFSCSSYSLVVLIVWFL 475

BLAST of Tan0007231 vs. ExPASy Swiss-Prot
Match: Q9M088 (Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 PE=2 SV=1)

HSP 1 Score: 502.3 bits (1292), Expect = 6.3e-141
Identity = 246/461 (53.36%), Postives = 323/461 (70.07%), Query Frame = 0

Query: 13  IFRTFLLLFLSIFPNGESVGVNWGTMATHQLPPEKVVKMLQENGFHKLKLFEAEDRILEA 72
           +F    LL+ S+    E +GVNWG+ A H LPP  VV++L+ENG  K+KLFEA+  IL+A
Sbjct: 9   VFFVITLLYASLLIEVEGIGVNWGSQARHPLPPATVVRLLRENGIQKVKLFEADSAILKA 68

Query: 73  LIGTDIEVMLAIPNSMLQIMSEDSEAAASWVDANVTAYTYHGGVNIKYVAVGNEPFLKSY 132
           L  T I+VM+ IPN +L  ++    AA  WV  NV+A+    GV+I+YVAVGNEPFLK++
Sbjct: 69  LSRTGIQVMVGIPNDLLAPLAGSVAAAERWVSQNVSAHVSSNGVDIRYVAVGNEPFLKAF 128

Query: 133 NGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRPDVRDL 192
           NGT+  +TLPAL+NIQ A+  AGL ++VK T+P NAD+Y S  ++ +PS GDFRP++RDL
Sbjct: 129 NGTFEGITLPALQNIQSAIIKAGLATQVKVTVPLNADVYQS--ASNLPSDGDFRPEIRDL 188

Query: 193 TIEILHFLYLNNAPFTVNIYPFLSLYGNDYFPMDFAFFDGTYKPIKDGDLTYTNVFDANF 252
            + I+ FL  N APFT+NIYPF+SLY +  FP++FAFFDGT  PI D    Y NV DAN+
Sbjct: 189 MLNIVKFLSDNGAPFTINIYPFISLYNDPNFPVEFAFFDGTGTPINDNGRIYDNVLDANY 248

Query: 253 DTLVSALVKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVGI 312
           DTLV +L K G+ ++ IIVGEVGWPTDGDK+AN+  A+R+NQG +    + +GTP R G 
Sbjct: 249 DTLVWSLQKNGFGNLTIIVGEVGWPTDGDKNANLMYARRYNQGFMNRQKANKGTPMRPGA 308

Query: 313 IDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLIPVEGVKYMVK 372
           +D YLF LIDE+AKSI+PG+FERHWGIF  DG+PKY+L L       GLIP + V Y+ K
Sbjct: 309 MDAYLFGLIDEDAKSIQPGNFERHWGIFYIDGQPKYQLSLG---SGNGLIPAKDVHYLAK 368

Query: 373 RWCILNPNVNDWE-GLADSVDYACSLSDCTALEYGSSCNQLTAQGNASYAFNMYYQVNSQ 432
           +WCIL PN N  +  L  SV YAC  +DCT+L YGSSC  L    N SYAFN YYQV++Q
Sbjct: 369 KWCILAPNANLQDPQLGPSVSYACDHADCTSLGYGSSCGNLNLAQNVSYAFNSYYQVSNQ 428

Query: 433 KSWNCDFSGLAMVTEEDPSYGNCQFPIMIDYTSSSSSPFLL 473
               C F GL++V+  DPS G+C+F IMI    +S +  ++
Sbjct: 429 LDSACKFPGLSIVSTRDPSVGSCKFKIMIKSEDASEASAMM 464

BLAST of Tan0007231 vs. ExPASy Swiss-Prot
Match: Q93Z08 (Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 PE=2 SV=2)

HSP 1 Score: 499.2 bits (1284), Expect = 5.3e-140
Identity = 245/445 (55.06%), Postives = 310/445 (69.66%), Query Frame = 0

Query: 18  LLLFLSIFPNGESVGVNWGTMATHQLPPEKVVKMLQENGFHKLKLFEAEDRILEALIGTD 77
           LL    +     S+G NWGT A+H LPP+ VV+ML+ENG  K+KLF+AE   L AL  + 
Sbjct: 9   LLAVALLCQRASSIGANWGTQASHPLPPDIVVRMLRENGIQKVKLFDAEYDTLRALGKSG 68

Query: 78  IEVMLAIPNSMLQIMSEDSEAAASWVDANVTAYTYHGGVNIKYVAVGNEPFLKSYNGTYL 137
           IEVM+ IPN ML  ++   +AA  WV  NV+ +     VNI+YVAVGNEPFL +YNG+YL
Sbjct: 69  IEVMVGIPNEMLATLASSLKAAEKWVAKNVSTHISTDNVNIRYVAVGNEPFLSTYNGSYL 128

Query: 138 QLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRPDVRDLTIEIL 197
             T PALRNIQ A+  AGL ++VK T P NAD+Y+S  S   PS GDFR ++RDL I I+
Sbjct: 129 STTFPALRNIQIAIIKAGLQNQVKVTCPLNADVYDS--STTFPSGGDFRANIRDLMITIV 188

Query: 198 HFLYLNNAPFTVNIYPFLSLYGNDYFPMDFAFFDGTYKPIKDGDLTYTNVFDANFDTLVS 257
            FL  N  PFTVNIYP++SLY N  FP+D+AFFDG  +P+ DG   Y N+FDAN+DTLV 
Sbjct: 189 KFLSENGGPFTVNIYPYISLYTNPDFPVDYAFFDGNAQPLNDGGTFYYNMFDANYDTLVH 248

Query: 258 ALVKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVGIIDIYL 317
           AL K G+ +M II+GE+GWPTDGD +AN+  AK+FNQG + H   G+GTP R G ID YL
Sbjct: 249 ALEKNGFGNMPIIIGEIGWPTDGDSNANLDYAKKFNQGFMAHISGGKGTPRRPGPIDAYL 308

Query: 318 FSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLIPVEGVKYMVKRWCIL 377
           FSLIDE+AKS++PG FERHWGIF FDG PKY L+L GT     LI  +GV+Y+ ++WC++
Sbjct: 309 FSLIDEDAKSVQPGYFERHWGIFTFDGLPKYALNL-GTTNTGALIQAKGVRYLERKWCVM 368

Query: 378 NPNVN-DWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASYAFNMYYQVNSQKSWNC 437
            PNV  D   +A +V YACSL DCT+L  G+SC  L  + N SYAFN YYQ+  Q    C
Sbjct: 369 KPNVRLDDPQVAPAVSYACSLGDCTSLGVGTSCANLDGKQNISYAFNSYYQIQDQLDTAC 428

Query: 438 DFSGLAMVTEEDPSYGNCQFPIMID 462
            F  ++ VT+ DPS G C+FPIMI+
Sbjct: 429 KFPNISEVTKTDPSTGTCRFPIMIE 450

BLAST of Tan0007231 vs. ExPASy Swiss-Prot
Match: Q9FGH4 (Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 PE=2 SV=1)

HSP 1 Score: 357.8 bits (917), Expect = 1.9e-97
Identity = 190/460 (41.30%), Postives = 283/460 (61.52%), Query Frame = 0

Query: 30  SVGVNWGTMATHQLPPEKVVKMLQENGFHKLKLFEAEDRILEALIGTDIEVMLAIPNSML 89
           +VG+NWGT A+H LPP KVV++L+ NG  K+KLF+A+ ++L AL G++I V + I NSML
Sbjct: 25  AVGINWGTEASHPLPPSKVVELLKSNGIVKVKLFDADPKVLRALSGSNIGVTIGIQNSML 84

Query: 90  QIMSEDSEAAASWVDANVTAYTYHGG--VNIKYVAVGNEPFLKSYNGTYLQLTLPALRNI 149
           + ++   + A SWV  NVT Y ++GG  V I+YVAVG EPFL+SY   Y    + A  NI
Sbjct: 85  KSLNASVKVAESWVHDNVTRY-FNGGNRVRIEYVAVGEEPFLQSYGNQYKPFVIGAAMNI 144

Query: 150 QQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRPDVRDLTIEILHFLYLNNAPF 209
           Q AL  A L ++VK  +P + D + S      PS+G FR D+    IE+L FL  +++PF
Sbjct: 145 QNALVKANLANEVKVVVPSSFDSFLSESGR--PSSGHFRADLNKTMIELLSFLTKHHSPF 204

Query: 210 TVNIYPFLSLYGNDYFPMDFAFFDGTYKPIKDGDLTYTNVFDANFDTLVSALVKAGYPDM 269
            V I PFLS + N    +DF+ F  T K  KDG  TY N FD ++DTLVSAL   G+ ++
Sbjct: 205 FVTISPFLSFHQNKNISLDFSLFKETAKAHKDGRKTYRNSFDLSYDTLVSALFTIGFSEV 264

Query: 270 KIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVGIIDIYLFSLIDENAKS 329
            I+V ++GWPTDG ++A    A+ F +GL+ H      +  R   ++ Y+ SL+DE+ ++
Sbjct: 265 DIVVSKIGWPTDGAENATSLTAEAFFKGLIVHLEKKTASLPRPP-VETYIESLLDEDQRN 324

Query: 330 IEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLIPVEGVKYMVKRWCILNPNVNDWEGL 389
           +  G+FERHWG+F FDG+ KY    +     K  +  + V+Y+  +WC++N N +     
Sbjct: 325 LSAGNFERHWGVFTFDGQAKYNFSFN----HKNQVNAQNVQYLPPKWCVVNNNKDLSNAS 384

Query: 390 ADSVDYACSLSDCTALEYGSSCNQLTAQGNASYAFNMYYQVNSQKSWNCDFSGLAMVTEE 449
           A +++ AC+++DCT++  G SC+ +   GN SYAFN  YQ N   + +C+F GL ++T  
Sbjct: 385 ARALE-ACAVADCTSILPGGSCSGIRWPGNVSYAFNSLYQQNDHSAESCNFGGLGLITTV 444

Query: 450 DPSYGNCQFPIMIDYTSSSSSPFLLDERFLDLLVAIFMGL 488
           DPS  NC+F I +D + SSS      + +  LL+ +  GL
Sbjct: 445 DPSEDNCRFSIQLDTSHSSSQTPNFFQSWPLLLLFLLSGL 475

BLAST of Tan0007231 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 243.8 bits (621), Expect = 4.0e-63
Identity = 160/459 (34.86%), Postives = 247/459 (53.81%), Query Frame = 0

Query: 17  FLLLFLSIFPNGES---VGVNWGTMATHQLPPEKVVKMLQENGFHKLKLFEAEDRILEAL 76
           FLL+FLS FP+  +   +GVN+G +A +  PP + VK+LQ     K++L+ A+  I++AL
Sbjct: 9   FLLIFLSHFPSSHAEPFIGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADPAIIKAL 68

Query: 77  IGTDIEVMLAIPNSMLQIMSEDSEAAASWVDANVTAYTYHGGVNIKYVAVGNEPFLKSYN 136
            GT + +++   N  +  ++ D  AA  W+++NV    ++    I  + VGNE  L S +
Sbjct: 69  AGTGVGIVIGAANGDVPSLASDPNAATQWINSNV--LPFYPASKIMLITVGNE-ILMSND 128

Query: 137 GTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRPDVRDLT 196
              +   LPA++N+Q+AL    LG K+K +   +  +  S D    PS+G F    +   
Sbjct: 129 PNLVNQLLPAMQNVQKALEAVSLGGKIKVSTVNSMTVLGSSDP---PSSGSFAAGYQTGL 188

Query: 197 IEILHFLYLNNAPFTVNIYPFLSLYGNDYFPMDFAF--FDGTYKPI--KDGDLTYTNVFD 256
             IL FL    +PF +N YPF + Y +D  P   AF  F+     +  K G + YTN+FD
Sbjct: 189 KGILQFLSDTGSPFAINPYPFFA-YQSDPRPETLAFCLFEPNAGRVDSKTG-IKYTNMFD 248

Query: 257 ANFDTLVSALVKAGYPDMKIIVGEVGWPTDGDKH---ANIQNAKRFNQGLLRHALSGEGT 316
           A  D + SAL   G+  ++I+V E GW + GD +   A++ NAK +N  L+ H  S  GT
Sbjct: 249 AQVDAVHSALKSMGFEKVEIVVAETGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGT 308

Query: 317 PARVG-IIDIYLFSLIDENAKSIEPG-SFERHWGIFEFDGKPKYELDL----SGTEEEKG 376
           P   G  +D Y+F+L DEN K   PG S ER +G+F+ D    Y++ L    S ++   G
Sbjct: 309 PLMPGKPVDTYIFALYDENLK---PGPSSERAFGLFKTDLSMVYDVGLAKSSSSSQTPSG 368

Query: 377 LIPVEGVKYMVKRWCILNPNVNDWEGLADSVDYACSLS-DCTALEYGSSC-NQLTAQGNA 436
            +   G       WC+      + E L  S+D+AC    DC A++ G +C        +A
Sbjct: 369 KVTSSG-------WCVPKKGATN-EELQASLDWACGHGIDCGAIQPGGACFEPNNVVSHA 428

Query: 437 SYAFNMYYQVNSQKSWNCDFSGLAMVTEEDPSYGNCQFP 458
           +YA NMY+Q + ++  +CDFS  A VT ++PSY NC +P
Sbjct: 429 AYAMNMYFQKSPKQPTDCDFSKTATVTSQNPSYNNCVYP 448

BLAST of Tan0007231 vs. NCBI nr
Match: XP_038890697.1 (glucan endo-1,3-beta-glucosidase 8-like [Benincasa hispida])

HSP 1 Score: 940.3 bits (2429), Expect = 6.9e-270
Identity = 458/497 (92.15%), Postives = 474/497 (95.37%), Query Frame = 0

Query: 1   MSKSGLQIHRT-CIF--RTFLLLFLSIFPNGESVGVNWGTMATHQLPPEKVVKMLQENGF 60
           MSKSGLQ HR  CIF  RT + L L IFP+G SVGVNWGTMATHQLPPEKVVKMLQENGF
Sbjct: 1   MSKSGLQFHRNYCIFRIRTLVFLLLMIFPDGLSVGVNWGTMATHQLPPEKVVKMLQENGF 60

Query: 61  HKLKLFEAEDRILEALIGTDIEVMLAIPNSMLQIMSEDSEAAASWVDANVTAYTYHGGVN 120
           HKLKLFEAEDRILEALIG+DIEVMLAIPNSMLQ+MS++  AAASWVD+NVTAYTYHGGV 
Sbjct: 61  HKLKLFEAEDRILEALIGSDIEVMLAIPNSMLQLMSQNPRAAASWVDSNVTAYTYHGGVK 120

Query: 121 IKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSN 180
           IKYVAVGNEPFLKSYNGTYLQLTLPALRNIQ ALNDAGLGSKVKATIPFNADIYNSPDSN
Sbjct: 121 IKYVAVGNEPFLKSYNGTYLQLTLPALRNIQAALNDAGLGSKVKATIPFNADIYNSPDSN 180

Query: 181 PVPSAGDFRPDVRDLTIEILHFLYLNNAPFTVNIYPFLSLYGNDYFPMDFAFFDGTYKPI 240
           PVPSAG+FRPDVRDLTIEILHFL +NNAPFTVNIYPFLSLYGNDYFPMDFAFFDGTYKPI
Sbjct: 181 PVPSAGEFRPDVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPMDFAFFDGTYKPI 240

Query: 241 KDGDLTYTNVFDANFDTLVSALVKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLL 300
           KDGDLTYTNVFDANFDTLVSAL KAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLL
Sbjct: 241 KDGDLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLL 300

Query: 301 RHALSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEE 360
           RHALSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEE
Sbjct: 301 RHALSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEE 360

Query: 361 EKGLIPVEGVKYMVKRWCILNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGN 420
           EKGLIPVEGV+YMVKRWCILNPNVNDWEGLADS+DYACSLSDCTALEYGSSCNQLTAQGN
Sbjct: 361 EKGLIPVEGVRYMVKRWCILNPNVNDWEGLADSIDYACSLSDCTALEYGSSCNQLTAQGN 420

Query: 421 ASYAFNMYYQVNSQKSWNCDFSGLAMVTEEDPSYGNCQFPIMIDYTSSSSSPFLLDERFL 480
           ASYAFNMYYQVNSQKSWNCDF GLA+VT++DPSYGNCQFPIMIDY SSS SPFL DERFL
Sbjct: 421 ASYAFNMYYQVNSQKSWNCDFDGLAVVTQQDPSYGNCQFPIMIDYASSSYSPFLFDERFL 480

Query: 481 DLLVAIFMGLLVFLFLE 495
           DLLV +FMG +VFL LE
Sbjct: 481 DLLVEVFMGFMVFLILE 497

BLAST of Tan0007231 vs. NCBI nr
Match: XP_022157557.1 (glucan endo-1,3-beta-glucosidase 8 [Momordica charantia])

HSP 1 Score: 937.2 bits (2421), Expect = 5.8e-269
Identity = 451/493 (91.48%), Postives = 473/493 (95.94%), Query Frame = 0

Query: 1   MSKSGLQIHRTCIFRTFLLLFLSIFPNGESVGVNWGTMATHQLPPEKVVKMLQENGFHKL 60
           MSKSGLQIHR CIF   +LL+ +IFPNGESVGVNWGTMATHQLPPEKVVKMLQENGF KL
Sbjct: 1   MSKSGLQIHRDCIFTALILLYSTIFPNGESVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQIMSEDSEAAASWVDANVTAYTYHGGVNIKY 120
           KLFEAEDRILEALIG+DIEVMLAIPNSMLQIMSED EAAASWVD+NVT Y+YHGGVNIKY
Sbjct: 61  KLFEAEDRILEALIGSDIEVMLAIPNSMLQIMSEDPEAAASWVDSNVTTYSYHGGVNIKY 120

Query: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180
           VAVGNEPFLKSYNGTYLQ TLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP
Sbjct: 121 VAVGNEPFLKSYNGTYLQFTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180

Query: 181 SAGDFRPDVRDLTIEILHFLYLNNAPFTVNIYPFLSLYGNDYFPMDFAFFDGTYKPIKDG 240
           SAG+FR DVRDLTIEILHFL  NNAPFTVNIYPFLSLY NDYFP+DFAFFDGTYKPI+DG
Sbjct: 181 SAGEFRSDVRDLTIEILHFLSTNNAPFTVNIYPFLSLYSNDYFPLDFAFFDGTYKPIRDG 240

Query: 241 DLTYTNVFDANFDTLVSALVKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHA 300
           DL YTNVFDANFDTLVSAL KAGYP+MKIIVGEVGWPTDGDKHANIQ AKRFNQGLLRHA
Sbjct: 241 DLAYTNVFDANFDTLVSALTKAGYPEMKIIVGEVGWPTDGDKHANIQYAKRFNQGLLRHA 300

Query: 301 LSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360
           LSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWG+FEFDGKPKYELDLSGTE+EKG
Sbjct: 301 LSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWGLFEFDGKPKYELDLSGTEQEKG 360

Query: 361 LIPVEGVKYMVKRWCILNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASY 420
           L+ VEGV+YMVKRWC+LNPN+NDWEGLAD+VDYACSLSDCTALEYGSSCNQLTAQGNASY
Sbjct: 361 LVAVEGVRYMVKRWCVLNPNLNDWEGLADNVDYACSLSDCTALEYGSSCNQLTAQGNASY 420

Query: 421 AFNMYYQVNSQKSWNCDFSGLAMVTEEDPSYGNCQFPIMIDYTSSSSSPFLLDERFLDLL 480
           AFNMYYQVNSQKSWNCDFSGLA+VTEEDPSYGNCQFP+MIDYTSSSSSPFL+DERFLDLL
Sbjct: 421 AFNMYYQVNSQKSWNCDFSGLAVVTEEDPSYGNCQFPVMIDYTSSSSSPFLVDERFLDLL 480

Query: 481 VAIFMGLLVFLFL 494
           VA+ MGLLV  F+
Sbjct: 481 VAVIMGLLVSFFV 493

BLAST of Tan0007231 vs. NCBI nr
Match: XP_022965692.1 (glucan endo-1,3-beta-glucosidase 8-like [Cucurbita maxima])

HSP 1 Score: 924.1 bits (2387), Expect = 5.1e-265
Identity = 449/494 (90.89%), Postives = 471/494 (95.34%), Query Frame = 0

Query: 1   MSKSGLQIHRTCIFRTFLLLFLSIFPNGESVGVNWGTMATHQLPPEKVVKMLQENGFHKL 60
           MSKSGLQIHR   FRT LLLFL+I PNGE+VGVNWGTMATHQLPPEKVVKMLQENGF KL
Sbjct: 1   MSKSGLQIHRNFTFRTILLLFLTIIPNGETVGVNWGTMATHQLPPEKVVKMLQENGFLKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQIMSEDSEAAASWVDANVTAYTYHGGVNIKY 120
           KLFEAEDRILEALIGTDIEVMLA+PNSMLQIMS+DS+AAASWVDANVTAYTYHGGVNIKY
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSDDSKAAASWVDANVTAYTYHGGVNIKY 120

Query: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180
           VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP
Sbjct: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180

Query: 181 SAGDFRPDVRDLTIEILHFLYLNNAPFTVNIYPFLSLYGNDYFPMDFAFFDGTYKPIKDG 240
           SAGDFRP+VRDLTIEILHFL +NNAPFTVNIYPFLSLYGNDYFP+DFAFF GTYKPIKDG
Sbjct: 181 SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFGGTYKPIKDG 240

Query: 241 DLTYTNVFDANFDTLVSALVKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHA 300
           DLTYTNVFDANFDTLVSAL KAGYPDMKIIVGE+GWPTDGDKHANIQ+AKRFNQGLLRHA
Sbjct: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA 300

Query: 301 LSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360
           LSGEGTPARVG+ID+YLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTE+EKG
Sbjct: 301 LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEQEKG 360

Query: 361 LIPVEGVKYMVKRWCILNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASY 420
           LI VEGVKYM K+WC+LNPNV DWEGLAD++DYACSLSDCTALEYGSSCNQLTA+GNASY
Sbjct: 361 LIAVEGVKYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY 420

Query: 421 AFNMYYQVNSQKSWNCDFSGLAMVTEEDPSYGNCQFPIMIDYTSSSSSPFLLDERFLDLL 480
           AFNMYYQ+NSQK WNC+FSGLAMVTE+DPSYGNCQFPIMIDY SSSSS       FLDLL
Sbjct: 421 AFNMYYQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSS-------FLDLL 480

Query: 481 VAIFMGLLVFLFLE 495
           VAIFMG +VFL LE
Sbjct: 481 VAIFMGFVVFLCLE 487

BLAST of Tan0007231 vs. NCBI nr
Match: XP_023537244.1 (glucan endo-1,3-beta-glucosidase 8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 919.5 bits (2375), Expect = 1.3e-263
Identity = 448/494 (90.69%), Postives = 469/494 (94.94%), Query Frame = 0

Query: 1   MSKSGLQIHRTCIFRTFLLLFLSIFPNGESVGVNWGTMATHQLPPEKVVKMLQENGFHKL 60
           MSK  LQIHR  I RT LLLFL+I PNGESVGVNWGTMATHQLPPEKVVKMLQENGF KL
Sbjct: 1   MSKFELQIHRNFILRTILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQIMSEDSEAAASWVDANVTAYTYHGGVNIKY 120
           KLFEAEDRILEALIGTDIEVMLA+PNSMLQIMSEDS+AAA WVDANVTAYTYHGGVNIKY
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSEDSKAAAYWVDANVTAYTYHGGVNIKY 120

Query: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180
           VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP
Sbjct: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180

Query: 181 SAGDFRPDVRDLTIEILHFLYLNNAPFTVNIYPFLSLYGNDYFPMDFAFFDGTYKPIKDG 240
           SAGDFRP+VRDLTIEILHFL +NNAPFTVNIYPFLSLYGNDYFP+DFAFFDGTYKPIKDG
Sbjct: 181 SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDG 240

Query: 241 DLTYTNVFDANFDTLVSALVKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHA 300
           DLTYTNVFDANFDTLVSAL KAGYPDMKIIVGE+GWPTDGDKHANIQ+AKRFNQGLLRHA
Sbjct: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA 300

Query: 301 LSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360
           LSGEGTPARVG+ID+YLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTE+EKG
Sbjct: 301 LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEQEKG 360

Query: 361 LIPVEGVKYMVKRWCILNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASY 420
           LI VEGVKYM K+WC+LNPNV DWEGLAD++DYACSLSDCTALEYGSSCNQLTA+GNASY
Sbjct: 361 LIAVEGVKYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY 420

Query: 421 AFNMYYQVNSQKSWNCDFSGLAMVTEEDPSYGNCQFPIMIDYTSSSSSPFLLDERFLDLL 480
           AFNMY+Q+NSQK WNC+FSGLAMVTE+DPSYGNCQFPIMIDY SSSSS       FLDLL
Sbjct: 421 AFNMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSS-------FLDLL 480

Query: 481 VAIFMGLLVFLFLE 495
           VAIFMG +VFL LE
Sbjct: 481 VAIFMGFVVFLCLE 487

BLAST of Tan0007231 vs. NCBI nr
Match: KAG7021271.1 (Glucan endo-1,3-beta-glucosidase 8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 919.5 bits (2375), Expect = 1.3e-263
Identity = 448/494 (90.69%), Postives = 469/494 (94.94%), Query Frame = 0

Query: 1   MSKSGLQIHRTCIFRTFLLLFLSIFPNGESVGVNWGTMATHQLPPEKVVKMLQENGFHKL 60
           MSK  LQIHR  I RT LLLFL+I PNGESVGVNWGTMATHQLPPEKVVKMLQENGF KL
Sbjct: 1   MSKFELQIHRNFILRTILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQIMSEDSEAAASWVDANVTAYTYHGGVNIKY 120
           KLFEAEDRILEALIGTDIEVMLA+PNSMLQIMSEDS+AAA WVDANVTAYTYHGGVNIKY
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSEDSKAAAYWVDANVTAYTYHGGVNIKY 120

Query: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180
           VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP
Sbjct: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180

Query: 181 SAGDFRPDVRDLTIEILHFLYLNNAPFTVNIYPFLSLYGNDYFPMDFAFFDGTYKPIKDG 240
           SAGDFRP+VRDLTIEILHFL +NNAPFTVNIYPFLSLYGNDYFP+DFAFFDGTYKPIKDG
Sbjct: 181 SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDG 240

Query: 241 DLTYTNVFDANFDTLVSALVKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHA 300
           DLTYTNVFDANFDTLVSAL KAGYPDMKIIVGE+GWPTDGDKHANIQ+AKRFNQGLLRHA
Sbjct: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA 300

Query: 301 LSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360
           LSGEGTPARVG+ID+YLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG
Sbjct: 301 LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360

Query: 361 LIPVEGVKYMVKRWCILNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASY 420
           LI VEGV+YM K+WC+LNPNV DWEGLAD++DYACSLSDCTALEYGSSCNQLTA+GNASY
Sbjct: 361 LIAVEGVRYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY 420

Query: 421 AFNMYYQVNSQKSWNCDFSGLAMVTEEDPSYGNCQFPIMIDYTSSSSSPFLLDERFLDLL 480
           AFNMY+Q+NSQK WNC+FSGLAMVTE+DPSYGNCQFPIMIDY SSSSS       FLDLL
Sbjct: 421 AFNMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSS-------FLDLL 480

Query: 481 VAIFMGLLVFLFLE 495
           VAIFMG +VFL LE
Sbjct: 481 VAIFMGFVVFLSLE 487

BLAST of Tan0007231 vs. ExPASy TrEMBL
Match: A0A6J1DUS6 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111024222 PE=3 SV=1)

HSP 1 Score: 937.2 bits (2421), Expect = 2.8e-269
Identity = 451/493 (91.48%), Postives = 473/493 (95.94%), Query Frame = 0

Query: 1   MSKSGLQIHRTCIFRTFLLLFLSIFPNGESVGVNWGTMATHQLPPEKVVKMLQENGFHKL 60
           MSKSGLQIHR CIF   +LL+ +IFPNGESVGVNWGTMATHQLPPEKVVKMLQENGF KL
Sbjct: 1   MSKSGLQIHRDCIFTALILLYSTIFPNGESVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQIMSEDSEAAASWVDANVTAYTYHGGVNIKY 120
           KLFEAEDRILEALIG+DIEVMLAIPNSMLQIMSED EAAASWVD+NVT Y+YHGGVNIKY
Sbjct: 61  KLFEAEDRILEALIGSDIEVMLAIPNSMLQIMSEDPEAAASWVDSNVTTYSYHGGVNIKY 120

Query: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180
           VAVGNEPFLKSYNGTYLQ TLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP
Sbjct: 121 VAVGNEPFLKSYNGTYLQFTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180

Query: 181 SAGDFRPDVRDLTIEILHFLYLNNAPFTVNIYPFLSLYGNDYFPMDFAFFDGTYKPIKDG 240
           SAG+FR DVRDLTIEILHFL  NNAPFTVNIYPFLSLY NDYFP+DFAFFDGTYKPI+DG
Sbjct: 181 SAGEFRSDVRDLTIEILHFLSTNNAPFTVNIYPFLSLYSNDYFPLDFAFFDGTYKPIRDG 240

Query: 241 DLTYTNVFDANFDTLVSALVKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHA 300
           DL YTNVFDANFDTLVSAL KAGYP+MKIIVGEVGWPTDGDKHANIQ AKRFNQGLLRHA
Sbjct: 241 DLAYTNVFDANFDTLVSALTKAGYPEMKIIVGEVGWPTDGDKHANIQYAKRFNQGLLRHA 300

Query: 301 LSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360
           LSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWG+FEFDGKPKYELDLSGTE+EKG
Sbjct: 301 LSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWGLFEFDGKPKYELDLSGTEQEKG 360

Query: 361 LIPVEGVKYMVKRWCILNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASY 420
           L+ VEGV+YMVKRWC+LNPN+NDWEGLAD+VDYACSLSDCTALEYGSSCNQLTAQGNASY
Sbjct: 361 LVAVEGVRYMVKRWCVLNPNLNDWEGLADNVDYACSLSDCTALEYGSSCNQLTAQGNASY 420

Query: 421 AFNMYYQVNSQKSWNCDFSGLAMVTEEDPSYGNCQFPIMIDYTSSSSSPFLLDERFLDLL 480
           AFNMYYQVNSQKSWNCDFSGLA+VTEEDPSYGNCQFP+MIDYTSSSSSPFL+DERFLDLL
Sbjct: 421 AFNMYYQVNSQKSWNCDFSGLAVVTEEDPSYGNCQFPVMIDYTSSSSSPFLVDERFLDLL 480

Query: 481 VAIFMGLLVFLFL 494
           VA+ MGLLV  F+
Sbjct: 481 VAVIMGLLVSFFV 493

BLAST of Tan0007231 vs. ExPASy TrEMBL
Match: A0A6J1HL09 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111465515 PE=3 SV=1)

HSP 1 Score: 924.1 bits (2387), Expect = 2.5e-265
Identity = 449/494 (90.89%), Postives = 471/494 (95.34%), Query Frame = 0

Query: 1   MSKSGLQIHRTCIFRTFLLLFLSIFPNGESVGVNWGTMATHQLPPEKVVKMLQENGFHKL 60
           MSKSGLQIHR   FRT LLLFL+I PNGE+VGVNWGTMATHQLPPEKVVKMLQENGF KL
Sbjct: 1   MSKSGLQIHRNFTFRTILLLFLTIIPNGETVGVNWGTMATHQLPPEKVVKMLQENGFLKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQIMSEDSEAAASWVDANVTAYTYHGGVNIKY 120
           KLFEAEDRILEALIGTDIEVMLA+PNSMLQIMS+DS+AAASWVDANVTAYTYHGGVNIKY
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSDDSKAAASWVDANVTAYTYHGGVNIKY 120

Query: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180
           VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP
Sbjct: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180

Query: 181 SAGDFRPDVRDLTIEILHFLYLNNAPFTVNIYPFLSLYGNDYFPMDFAFFDGTYKPIKDG 240
           SAGDFRP+VRDLTIEILHFL +NNAPFTVNIYPFLSLYGNDYFP+DFAFF GTYKPIKDG
Sbjct: 181 SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFGGTYKPIKDG 240

Query: 241 DLTYTNVFDANFDTLVSALVKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHA 300
           DLTYTNVFDANFDTLVSAL KAGYPDMKIIVGE+GWPTDGDKHANIQ+AKRFNQGLLRHA
Sbjct: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA 300

Query: 301 LSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360
           LSGEGTPARVG+ID+YLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTE+EKG
Sbjct: 301 LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEQEKG 360

Query: 361 LIPVEGVKYMVKRWCILNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASY 420
           LI VEGVKYM K+WC+LNPNV DWEGLAD++DYACSLSDCTALEYGSSCNQLTA+GNASY
Sbjct: 361 LIAVEGVKYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY 420

Query: 421 AFNMYYQVNSQKSWNCDFSGLAMVTEEDPSYGNCQFPIMIDYTSSSSSPFLLDERFLDLL 480
           AFNMYYQ+NSQK WNC+FSGLAMVTE+DPSYGNCQFPIMIDY SSSSS       FLDLL
Sbjct: 421 AFNMYYQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSS-------FLDLL 480

Query: 481 VAIFMGLLVFLFLE 495
           VAIFMG +VFL LE
Sbjct: 481 VAIFMGFVVFLCLE 487

BLAST of Tan0007231 vs. ExPASy TrEMBL
Match: A0A6J1FB83 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111443850 PE=3 SV=1)

HSP 1 Score: 917.1 bits (2369), Expect = 3.0e-263
Identity = 446/494 (90.28%), Postives = 469/494 (94.94%), Query Frame = 0

Query: 1   MSKSGLQIHRTCIFRTFLLLFLSIFPNGESVGVNWGTMATHQLPPEKVVKMLQENGFHKL 60
           MSK  LQIHR  I RT LLLFL+I PNGESVGVNWGTMATHQLPPEKVVKMLQENGF KL
Sbjct: 1   MSKFELQIHRNFILRTILLLFLTIIPNGESVGVNWGTMATHQLPPEKVVKMLQENGFLKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQIMSEDSEAAASWVDANVTAYTYHGGVNIKY 120
           KLFEAEDRILEALIGTDIEVMLA+PNSMLQIMS+DS+AAA WVDANVTAYTYHGGVNIKY
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLALPNSMLQIMSDDSKAAAYWVDANVTAYTYHGGVNIKY 120

Query: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180
           VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP
Sbjct: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180

Query: 181 SAGDFRPDVRDLTIEILHFLYLNNAPFTVNIYPFLSLYGNDYFPMDFAFFDGTYKPIKDG 240
           SAGDFRP+VRDLTIEILHFL +NNAPFTVNIYPFLSLYGNDYFP+DFAFFDGTYKPIKDG
Sbjct: 181 SAGDFRPEVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKPIKDG 240

Query: 241 DLTYTNVFDANFDTLVSALVKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHA 300
           DLTYTNVFDANFDTLVSAL KAGYPDMKIIVGE+GWPTDGDKHANIQ+AKRFNQGLLRHA
Sbjct: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEIGWPTDGDKHANIQDAKRFNQGLLRHA 300

Query: 301 LSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360
           LSGEGTPARVG+ID+YLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTE+EKG
Sbjct: 301 LSGEGTPARVGVIDVYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEQEKG 360

Query: 361 LIPVEGVKYMVKRWCILNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASY 420
           LI VEGV+YM K+WC+LNPNV DWEGLAD++DYACSLSDCTALEYGSSCNQLTA+GNASY
Sbjct: 361 LIAVEGVRYMDKKWCVLNPNVKDWEGLADNIDYACSLSDCTALEYGSSCNQLTAEGNASY 420

Query: 421 AFNMYYQVNSQKSWNCDFSGLAMVTEEDPSYGNCQFPIMIDYTSSSSSPFLLDERFLDLL 480
           AFNMY+Q+NSQK WNC+FSGLAMVTE+DPSYGNCQFPIMIDY SSSSS       FLDLL
Sbjct: 421 AFNMYFQMNSQKRWNCEFSGLAMVTEQDPSYGNCQFPIMIDYNSSSSS-------FLDLL 480

Query: 481 VAIFMGLLVFLFLE 495
           VAIFMG +VFL LE
Sbjct: 481 VAIFMGFVVFLSLE 487

BLAST of Tan0007231 vs. ExPASy TrEMBL
Match: A0A1S3C742 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103497605 PE=3 SV=1)

HSP 1 Score: 906.7 bits (2342), Expect = 4.1e-260
Identity = 444/491 (90.43%), Postives = 465/491 (94.70%), Query Frame = 0

Query: 10  RTCIFR---TFLLLFLSIFPNGESVGVNWGTMATHQLPPEKVVKMLQENGFHKLKLFEAE 69
           R CIFR     LLL L I PNG SVGVNWGTMATHQLPPEKVVKMLQENGFHKLKLFEA+
Sbjct: 3   RNCIFRILLVLLLLLLMIVPNGSSVGVNWGTMATHQLPPEKVVKMLQENGFHKLKLFEAD 62

Query: 70  DRILEALIGTDIEVMLAIPNSMLQIMSEDSEAAASWVDANVTAYTYHGGVNIKYVAVGNE 129
           DRILEALIGTDIEVMLAIPNSML +MS+D +AAASWVD+NVTAYTYHGGV IKYVAVGNE
Sbjct: 63  DRILEALIGTDIEVMLAIPNSMLFLMSQDPQAAASWVDSNVTAYTYHGGVKIKYVAVGNE 122

Query: 130 PFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFR 189
           PFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAG+FR
Sbjct: 123 PFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGEFR 182

Query: 190 PDVRDLTIEILHFLYLNNAPFTVNIYPFLSLYGNDYFPMDFAFFDGT-YKPIKDGDLTYT 249
           PDVRDL IEILH+L +NNAPFTVNIYPFLSLYGNDYFP+DFAFFDGT YKPIKD DLTYT
Sbjct: 183 PDVRDLAIEILHYLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTAYKPIKDRDLTYT 242

Query: 250 NVFDANFDTLVSALVKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEG 309
           NVFDANFDTLVSAL KAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEG
Sbjct: 243 NVFDANFDTLVSALAKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEG 302

Query: 310 TPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLIPVE 369
           TPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDL+GTEEEKGLIPVE
Sbjct: 303 TPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLAGTEEEKGLIPVE 362

Query: 370 GVKYMVKRWCILNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASYAFNMY 429
           GV+YM KRWC+LNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASYAFNMY
Sbjct: 363 GVRYMGKRWCVLNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASYAFNMY 422

Query: 430 YQVNSQKSWNCDFSGLAMVTEEDPSYGNCQFPIMIDYT--SSSSSPFLLDERFLDLLVAI 489
           YQVN+QKSWNCDF GLA+VT++DPSYGNC FPIMIDYT  SSSSS FL+DERF DLLVAI
Sbjct: 423 YQVNNQKSWNCDFDGLAVVTQQDPSYGNCLFPIMIDYTSSSSSSSSFLVDERFFDLLVAI 482

Query: 490 FMGLLVFLFLE 495
           FMG++VFL ++
Sbjct: 483 FMGIVVFLMVQ 493

BLAST of Tan0007231 vs. ExPASy TrEMBL
Match: A0A6J1G3H9 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111450439 PE=3 SV=1)

HSP 1 Score: 897.9 bits (2319), Expect = 1.9e-257
Identity = 438/494 (88.66%), Postives = 459/494 (92.91%), Query Frame = 0

Query: 1   MSKSGLQIHRTCIFRTFLLLFLSIFPNGESVGVNWGTMATHQLPPEKVVKMLQENGFHKL 60
           MS+S L+ HR  IF   +LL L++ PNG SVGVNWGTMATHQLPPEKVVKMLQENGF KL
Sbjct: 1   MSESVLRFHRNWIFIALILLLLTVVPNGFSVGVNWGTMATHQLPPEKVVKMLQENGFRKL 60

Query: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQIMSEDSEAAASWVDANVTAYTYHGGVNIKY 120
           KLFEAEDRILEALIGTDIEVMLAIPNSMLQ+MSED EAAA WVD+NVTAYTYHGGV IKY
Sbjct: 61  KLFEAEDRILEALIGTDIEVMLAIPNSMLQVMSEDPEAAALWVDSNVTAYTYHGGVKIKY 120

Query: 121 VAVGNEPFLKSYNGTYLQLTLPALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVP 180
           VAVGNEPFLKSYNG YLQ TLPALRNIQQALNDAGLGSKVKATIPFNAD YNSPDSNPVP
Sbjct: 121 VAVGNEPFLKSYNGIYLQSTLPALRNIQQALNDAGLGSKVKATIPFNADTYNSPDSNPVP 180

Query: 181 SAGDFRPDVRDLTIEILHFLYLNNAPFTVNIYPFLSLYGNDYFPMDFAFFDGTYKPIKDG 240
           SAGDFRPDVRDLTIEILHFL +NNAPFTVNIYPFLSLYGNDYFP+DFAFFDGTYK IKDG
Sbjct: 181 SAGDFRPDVRDLTIEILHFLSINNAPFTVNIYPFLSLYGNDYFPIDFAFFDGTYKSIKDG 240

Query: 241 DLTYTNVFDANFDTLVSALVKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHA 300
           DLTYTNVFDANFDTLVSAL KAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHA
Sbjct: 241 DLTYTNVFDANFDTLVSALTKAGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHA 300

Query: 301 LSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKG 360
           LSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIF+FDGKPKYELDLSG E +KG
Sbjct: 301 LSGEGTPARVGIIDIYLFSLIDENAKSIEPGSFERHWGIFKFDGKPKYELDLSGIEAQKG 360

Query: 361 LIPVEGVKYMVKRWCILNPNVNDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASY 420
           LI VEGV+YMVKRWC+LNPNV DWEGLA SVDYACSLSDCTALEYGSSCNQL+A+GNASY
Sbjct: 361 LIAVEGVRYMVKRWCVLNPNVKDWEGLAYSVDYACSLSDCTALEYGSSCNQLSAEGNASY 420

Query: 421 AFNMYYQVNSQKSWNCDFSGLAMVTEEDPSYGNCQFPIMIDYTSSSSSPFLLDERFLDLL 480
           AFNMYYQ+NSQKSWNCDFSG+A+VT+EDPSYG+CQFP+MIDYT SSSS       FLDLL
Sbjct: 421 AFNMYYQMNSQKSWNCDFSGMAVVTQEDPSYGSCQFPVMIDYTFSSSSSSSSSSSFLDLL 480

Query: 481 VAIFMGLLVFLFLE 495
           VA FMG LVFLFLE
Sbjct: 481 VAGFMGFLVFLFLE 494

BLAST of Tan0007231 vs. TAIR 10
Match: AT3G24330.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 652.1 bits (1681), Expect = 3.5e-187
Identity = 311/468 (66.45%), Postives = 385/468 (82.26%), Query Frame = 0

Query: 27  NGESVGVNWGTMATHQLPPEKVVKMLQENGFHKLKLFEAEDRILEALIGTDIEVMLAIPN 86
           N  +VGVNWG MA+HQLPPEKVVKML +N F KLKLFEA+  IL+ALIG+DIEVM+ IPN
Sbjct: 35  NTSNVGVNWGIMASHQLPPEKVVKMLMDNSFTKLKLFEADQNILDALIGSDIEVMIGIPN 94

Query: 87  SMLQIMSEDSEAAASWVDANVTAYTYHGGVNIKYVAVGNEPFLKSYNGTYLQLTLPALRN 146
             L+ M++D+  AASWV+ NVTAY+Y+GGVNIKY+AVGNEPFL++YNGTY++ TLPAL N
Sbjct: 95  RFLKEMAQDTSVAASWVEENVTAYSYNGGVNIKYIAVGNEPFLQTYNGTYVEFTLPALIN 154

Query: 147 IQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRPDVRDLTIEILHFLYLNNAP 206
           IQ+AL +A L   VK T+PFNADIY SP++NPVPSAGDFRP++RD TIEI++FLY +++P
Sbjct: 155 IQRALEEADL-KNVKVTVPFNADIYFSPEANPVPSAGDFRPELRDATIEIINFLYSHDSP 214

Query: 207 FTVNIYPFLSLYGNDYFPMDFAFFDGTYKPIKDGDLTYTNVFDANFDTLVSALVKAGYPD 266
           FTVNIYPFLSLYGN YFP+DFAFFDGT K ++DG+L YTNVFDAN DTL+ A+ +  +  
Sbjct: 215 FTVNIYPFLSLYGNAYFPLDFAFFDGTNKSLRDGNLVYTNVFDANLDTLICAMERYSFLG 274

Query: 267 MKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVGII-DIYLFSLIDENA 326
           MKIIVGEVGWPTDGDK+AN+++AKRFNQG+++HA+SG GTPAR G+I D+YLFSL+DE+A
Sbjct: 275 MKIIVGEVGWPTDGDKNANVKSAKRFNQGMVKHAMSGNGTPARKGVIMDVYLFSLVDEDA 334

Query: 327 KSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLIPVEGVKYMVKRWCILNPNVNDWE 386
           KSI PG+FERHWGIFEFDG+PKYELDLSG   +K L+PVE VKY+ K WCIL+PN  + +
Sbjct: 335 KSIAPGTFERHWGIFEFDGRPKYELDLSGKGNDKPLVPVEDVKYLPKTWCILDPNAYNLD 394

Query: 387 GLADSVDYACSLSDCTALEYGSSCNQLTAQGNASYAFNMYYQVNSQKSWNCDFSGLAMVT 446
            L D++DYACSLSDCTAL YGSSCN LTA GN SYAFNMYYQ++ QK+W+CDF GL ++T
Sbjct: 395 DLPDNIDYACSLSDCTALGYGSSCNHLTATGNVSYAFNMYYQMHDQKTWDCDFLGLGLIT 454

Query: 447 EEDPSYGNCQFPIMIDYTSSSSSPFLLDERFLDLLVAIFMGLLVFLFL 494
           +EDPS   C+FP+MID T  S+       R L  + A  + +LV   L
Sbjct: 455 DEDPSDELCEFPVMID-TGDSTRLQPGSSRVLTRVAAAVLVMLVLPIL 500

BLAST of Tan0007231 vs. TAIR 10
Match: AT2G19440.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 551.2 bits (1419), Expect = 8.4e-157
Identity = 264/439 (60.14%), Postives = 329/439 (74.94%), Query Frame = 0

Query: 29  ESVGVNWGTMATHQLPPEKVVKMLQENGFHKLKLFEAEDRILEALIGTDIEVMLAIPNSM 88
           + +GVNWGTMATH+LPP+KVV+ML++N  +K+KLF+A++  + AL G+ +EVM+AIPN  
Sbjct: 19  DGLGVNWGTMATHKLPPKKVVQMLKDNNINKVKLFDADETTMSALSGSGLEVMVAIPNDQ 78

Query: 89  LQIMSEDSEAAASWVDANVTAYTYHGGVNIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQ 148
           L++M    + A  WV  NVT Y ++GGVNI +VAVGNEPFLKSYNG+++ LT PAL+NIQ
Sbjct: 79  LKVMG-SYDRAKDWVHKNVTRYNFNGGVNITFVAVGNEPFLKSYNGSFINLTFPALQNIQ 138

Query: 149 QALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRPDVRDLTIEILHFLYLNNAPFT 208
            ALN+AGLGS VKAT+P NAD+Y+SP SNPVPSAG FRPD+     +I+ FL  N+AP T
Sbjct: 139 NALNEAGLGSSVKATVPLNADVYDSPSSNPVPSAGRFRPDIIGQMTQIVDFLGNNSAPIT 198

Query: 209 VNIYPFLSLYGNDYFPMDFAFFDGTYKPIKDGDLTYTNVFDANFDTLVSALVKAGYPDMK 268
           +NIYPFLSLYGND FP+++AFFDG  KP+ D  + YTNVFDANFDTLVSAL   G+ DM 
Sbjct: 199 INIYPFLSLYGNDDFPLNYAFFDGA-KPVDDNGIAYTNVFDANFDTLVSALKAVGHGDMP 258

Query: 269 IIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVGIIDIYLFSLIDENAKSI 328
           IIVGEVGWPT+GDKHAN  +A RF  GLL       GTP R   I++YLF L+DE+AKSI
Sbjct: 259 IIVGEVGWPTEGDKHANSGSAYRFYNGLLPRLGENRGTPLRPTYIEVYLFGLLDEDAKSI 318

Query: 329 EPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLIPVEGVKYMVKRWCILNPNVNDWEGLA 388
            PG FERHWGIF+FDG+PK+ +DLSG  + K LI  E V Y  K+WC+ N    D   LA
Sbjct: 319 APGEFERHWGIFKFDGQPKFPIDLSGQGQNKLLIGAENVTYQPKKWCMFNTEAKDLTKLA 378

Query: 389 DSVDYACSLSDCTALEYGSSCNQLTAQGNASYAFNMYYQVNSQKSWNCDFSGLAMVTEED 448
            ++DYAC+ SDCTAL YGSSCN L A GNASYAFNMY+QV +Q    C F GLA +T ++
Sbjct: 379 ANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMYFQVKNQDEDACIFQGLATITTKN 438

Query: 449 PSYGNCQFPIMIDYTSSSS 468
            S G C FPI I  +++SS
Sbjct: 439 ISQGQCNFPIQIVASTASS 455

BLAST of Tan0007231 vs. TAIR 10
Match: AT1G64760.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 547.0 bits (1408), Expect = 1.6e-155
Identity = 269/464 (57.97%), Postives = 339/464 (73.06%), Query Frame = 0

Query: 22  LSIFPNGESVGVNWGTMATHQLPPEKVVKMLQENGFHKLKLFEAEDRILEALIGTDIEVM 81
           L I  NG  +GVNWGTMATH+LPP+ VV+ML++N  +K+KLF+A++  + AL G+ +EVM
Sbjct: 18  LGILVNG--LGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGSGLEVM 77

Query: 82  LAIPNSMLQIMSEDSEAAASWVDANVTAYTYHGGVNIKYVAVGNEPFLKSYNGTYLQLTL 141
           +AIPN  L++M+   + A  WV  NVT Y + GGVNI +VAVGNEPFLKSYNG+++ LT 
Sbjct: 78  VAIPNDQLKVMT-SYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTF 137

Query: 142 PALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRPDVRDLTIEILHFLY 201
           PAL NIQ ALN+AGLG+ VKAT+P NAD+Y+SP SNPVPSAG FRPD+     +I+ FL 
Sbjct: 138 PALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLG 197

Query: 202 LNNAPFTVNIYPFLSLYGNDYFPMDFAFFDGTYKPIKDGDLTYTNVFDANFDTLVSALVK 261
            NNAP T+NIYPFLSLYGND FP+++AFFDG  +PI D  + YTNVFDANFDTLVS+L  
Sbjct: 198 KNNAPITINIYPFLSLYGNDDFPLNYAFFDGA-EPINDNGIDYTNVFDANFDTLVSSLKA 257

Query: 262 AGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVGIIDIYLFSLI 321
            G+ DM IIVGEVGWPT+GDKHAN  +A RF  GLL    + +GTP R   I++YLF L+
Sbjct: 258 VGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEVYLFGLL 317

Query: 322 DENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLIPVEGVKYMVKRWCILNPNV 381
           DE+AKSI PG FERHWGIF+FDG+PK+ +DLSG  + K LI  + V Y+  +WC  NP  
Sbjct: 318 DEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEA 377

Query: 382 NDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASYAFNMYYQVNSQKSWNCDFSGL 441
            D   LA ++DYAC+ SDCTAL YGSSCN L A GNASYAFNM++QV +Q    C F GL
Sbjct: 378 KDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESACYFQGL 437

Query: 442 AMVTEEDPSYGNCQFPIMIDYTSSSSSPFLLDERFLDLLVAIFM 486
           A +T ++ S G C FPI I   +SS+S F      L +L+  F+
Sbjct: 438 ATITTQNISQGQCNFPIQI--VASSASSFSCSSYSLVVLIVWFL 475

BLAST of Tan0007231 vs. TAIR 10
Match: AT1G64760.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 547.0 bits (1408), Expect = 1.6e-155
Identity = 269/464 (57.97%), Postives = 339/464 (73.06%), Query Frame = 0

Query: 22  LSIFPNGESVGVNWGTMATHQLPPEKVVKMLQENGFHKLKLFEAEDRILEALIGTDIEVM 81
           L I  NG  +GVNWGTMATH+LPP+ VV+ML++N  +K+KLF+A++  + AL G+ +EVM
Sbjct: 18  LGILVNG--LGVNWGTMATHKLPPKTVVQMLKDNNINKVKLFDADETTMGALAGSGLEVM 77

Query: 82  LAIPNSMLQIMSEDSEAAASWVDANVTAYTYHGGVNIKYVAVGNEPFLKSYNGTYLQLTL 141
           +AIPN  L++M+   + A  WV  NVT Y + GGVNI +VAVGNEPFLKSYNG+++ LT 
Sbjct: 78  VAIPNDQLKVMT-SYDRAKDWVRKNVTRYNFDGGVNITFVAVGNEPFLKSYNGSFINLTF 137

Query: 142 PALRNIQQALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRPDVRDLTIEILHFLY 201
           PAL NIQ ALN+AGLG+ VKAT+P NAD+Y+SP SNPVPSAG FRPD+     +I+ FL 
Sbjct: 138 PALANIQNALNEAGLGNSVKATVPLNADVYDSPASNPVPSAGRFRPDIIGQMTQIVDFLG 197

Query: 202 LNNAPFTVNIYPFLSLYGNDYFPMDFAFFDGTYKPIKDGDLTYTNVFDANFDTLVSALVK 261
            NNAP T+NIYPFLSLYGND FP+++AFFDG  +PI D  + YTNVFDANFDTLVS+L  
Sbjct: 198 KNNAPITINIYPFLSLYGNDDFPLNYAFFDGA-EPINDNGIDYTNVFDANFDTLVSSLKA 257

Query: 262 AGYPDMKIIVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVGIIDIYLFSLI 321
            G+ DM IIVGEVGWPT+GDKHAN  +A RF  GLL    + +GTP R   I++YLF L+
Sbjct: 258 VGHGDMPIIVGEVGWPTEGDKHANAGSAYRFYNGLLPRLGTNKGTPLRPTYIEVYLFGLL 317

Query: 322 DENAKSIEPGSFERHWGIFEFDGKPKYELDLSGTEEEKGLIPVEGVKYMVKRWCILNPNV 381
           DE+AKSI PG FERHWGIF+FDG+PK+ +DLSG  + K LI  + V Y+  +WC  NP  
Sbjct: 318 DEDAKSIAPGPFERHWGIFKFDGQPKFPIDLSGQGQSKFLIGAQNVPYLPNKWCTFNPEA 377

Query: 382 NDWEGLADSVDYACSLSDCTALEYGSSCNQLTAQGNASYAFNMYYQVNSQKSWNCDFSGL 441
            D   LA ++DYAC+ SDCTAL YGSSCN L A GNASYAFNM++QV +Q    C F GL
Sbjct: 378 KDLTKLAANIDYACTFSDCTALGYGSSCNTLDANGNASYAFNMFFQVKNQDESACYFQGL 437

Query: 442 AMVTEEDPSYGNCQFPIMIDYTSSSSSPFLLDERFLDLLVAIFM 486
           A +T ++ S G C FPI I   +SS+S F      L +L+  F+
Sbjct: 438 ATITTQNISQGQCNFPIQI--VASSASSFSCSSYSLVVLIVWFL 475

BLAST of Tan0007231 vs. TAIR 10
Match: AT5G18220.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 503.1 bits (1294), Expect = 2.6e-142
Identity = 250/443 (56.43%), Postives = 320/443 (72.23%), Query Frame = 0

Query: 30  SVGVNWGTMATHQLPPEKVVKMLQENGFHKLKLFEAEDRILEALIGTDIEVMLAIPNSML 89
           ++GVNWGTM+THQLPP+ VV+ML++N   K+KLF+A+   + AL G+ IEVM+AIPN  L
Sbjct: 29  ALGVNWGTMSTHQLPPKTVVQMLKDNNVKKVKLFDADTNTMVALAGSGIEVMVAIPNDQL 88

Query: 90  QIMSEDSEAAASWVDANVTAYTYHGGVNIKYVAVGNEPFLKSYNGTYLQLTLPALRNIQQ 149
           + M   +  A  WV  N+T   ++  V IKYVAVGNEPFL +YNG+++ LT PAL NIQ+
Sbjct: 89  KAMGSYNR-AKDWVRRNIT--RFNDDVKIKYVAVGNEPFLTAYNGSFINLTYPALFNIQK 148

Query: 150 ALNDAGLGSKVKATIPFNADIYNSPDSNPVPSAGDFRPDVRDLTIEILHFLYLNNAPFTV 209
           ALN+AG+G  +KAT+P NAD+YNSP  NPVPSAG FR D+ +    I++FL  N APFTV
Sbjct: 149 ALNEAGVGDFIKATVPLNADVYNSPLENPVPSAGSFRQDIFEEMKLIVNFLAHNKAPFTV 208

Query: 210 NIYPFLSLYGNDYFPMDFAFFDGTYKPIKDGDLTYTNVFDANFDTLVSALVKAGYPDMKI 269
           NIYPFLSLY +  FP D+AFF+G    + D  + YTNVFDANFDTL+++L   G+ DM +
Sbjct: 209 NIYPFLSLYLSSDFPFDYAFFNG-QNTVSDNGVIYTNVFDANFDTLLASLKALGHGDMTV 268

Query: 270 IVGEVGWPTDGDKHANIQNAKRFNQGLLRHALSGEGTPARVGIIDIYLFSLIDENAKSIE 329
           IVGEVGWPTDGDK+ANI NA+RF  GLL    +  GTP R G I++YLF  IDE+AKSI 
Sbjct: 269 IVGEVGWPTDGDKNANIPNAERFYTGLLPKLAANRGTPMRPGYIEVYLFGFIDEDAKSIA 328

Query: 330 PGSFERHWGIFEFDGKPKYELDLSGTEEEKGLIPVEGVKYMVKRWCILNPNV----NDWE 389
           PG+FERHWGIF++DG+PK+  DLSG  ++K L   + V+Y+  +WC+ NPN     N+  
Sbjct: 329 PGNFERHWGIFKYDGQPKFPADLSGAGQKKILTGAQNVQYLRNQWCMFNPNALTFSNNTN 388

Query: 390 GLADSVDYACSLSDCTALEYGSSCNQLTAQGNASYAFNMYYQVNSQKSWNCDFSGLAMVT 449
            L D+V+YAC+ SDCTAL YGSSC  L   GNASYAFNMY+QV +QK+  CDF GLA++T
Sbjct: 389 QLGDNVNYACTFSDCTALGYGSSCGNLDEVGNASYAFNMYFQVQNQKAEACDFEGLAIIT 448

Query: 450 EEDPSYGNCQFPIMI-DYTSSSS 468
             + S   C FPI I D TS  S
Sbjct: 449 TRNISREQCNFPIQIGDPTSGHS 467

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6NKW92.2e-15457.97Glucan endo-1,3-beta-glucosidase 8 OS=Arabidopsis thaliana OX=3702 GN=At1g64760 ... [more]
Q9M0886.3e-14153.36Glucan endo-1,3-beta-glucosidase 5 OS=Arabidopsis thaliana OX=3702 GN=At4g31140 ... [more]
Q93Z085.3e-14055.06Glucan endo-1,3-beta-glucosidase 6 OS=Arabidopsis thaliana OX=3702 GN=At5g58090 ... [more]
Q9FGH41.9e-9741.30Glucan endo-1,3-beta-glucosidase 9 OS=Arabidopsis thaliana OX=3702 GN=At5g58480 ... [more]
Q9M0694.0e-6334.86Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Match NameE-valueIdentityDescription
XP_038890697.16.9e-27092.15glucan endo-1,3-beta-glucosidase 8-like [Benincasa hispida][more]
XP_022157557.15.8e-26991.48glucan endo-1,3-beta-glucosidase 8 [Momordica charantia][more]
XP_022965692.15.1e-26590.89glucan endo-1,3-beta-glucosidase 8-like [Cucurbita maxima][more]
XP_023537244.11.3e-26390.69glucan endo-1,3-beta-glucosidase 8-like [Cucurbita pepo subsp. pepo][more]
KAG7021271.11.3e-26390.69Glucan endo-1,3-beta-glucosidase 8 [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
A0A6J1DUS62.8e-26991.48(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111024222 ... [more]
A0A6J1HL092.5e-26590.89(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111465515 PE=... [more]
A0A6J1FB833.0e-26390.28(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111443850 P... [more]
A0A1S3C7424.1e-26090.43(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103497605 PE=3 SV... [more]
A0A6J1G3H91.9e-25788.66(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111450439 P... [more]
Match NameE-valueIdentityDescription
AT3G24330.13.5e-18766.45O-Glycosyl hydrolases family 17 protein [more]
AT2G19440.18.4e-15760.14O-Glycosyl hydrolases family 17 protein [more]
AT1G64760.11.6e-15557.97O-Glycosyl hydrolases family 17 protein [more]
AT1G64760.21.6e-15557.97O-Glycosyl hydrolases family 17 protein [more]
AT5G18220.12.6e-14256.43O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 373..456
e-value: 1.1E-36
score: 137.9
IPR012946X8 domainPFAMPF07983X8coord: 374..443
e-value: 4.9E-18
score: 65.6
NoneNo IPR availableGENE3D1.20.58.1040coord: 368..464
e-value: 2.6E-8
score: 36.1
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 29..359
e-value: 1.2E-110
score: 371.5
NoneNo IPR availablePANTHERPTHR32227:SF319BETA-1,3-GLUCANASE-LIKE PROTEINcoord: 16..467
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 31..351
e-value: 6.2E-66
score: 223.1
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 267..280
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 16..467
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 31..351

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0007231.1Tan0007231.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds