Tan0007056 (gene) Snake gourd v1

Overview
NameTan0007056
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionmetalloendoproteinase 1-MMP-like
LocationLG07: 69622415 .. 69623935 (+)
RNA-Seq ExpressionTan0007056
SyntenyTan0007056
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTGTTTACCAAACATTAACCAAAATAAAATTTGACTGAAAAAAAAAAACCAAAATAAAACAACATAGCAAAGTCAGAATCTACAATATGAAGGCAAGCACGCCAGAATCTTCTTAACTTTCCCATCATTCTCTGTTTTCTCTTCACCTTCCTCAAATCCAATTTCTAAGAACAGTTAATTAATTAAAGTAATTCATCAATTTATTTTTTTTAGATTCTAAAAACAAAATACTAATAAATAAATCACAAAATCGATTGGTGATAAAGCTTTTTTCCTTTCGATTTCTCTATTGTCCGATAACTGCCAAATAATATCTCCATGTTTCCGTTTTTCCGTTATTACCTCTTCTTCTTCTACCTTCTCTCCTCTGTTTTTCTCTTCTCCTCTGTTTTTCCCGCCAGAACTTTGCCGGACTTCACGACGGCGACTGACACAGACAACAACTACGCCTGGAGAAACTTCGCTCGGTTCCTCGATGCCGGAAAGGGCAGCGAAGTGACCGGCATGTCGGAGCTGAAGAAGTATTTGAATCGATTCGGTTACCTTCCGATTCCTCCAGAGAATAGCTTCTCCGATTGCTTCGACGACCAATTCGTATCGGCGTTGATTCTGTACCAGAAGCGCTTAGGTTTATCAGTCACCGGAAAGCTCGATTCTGAAACGATCGCGAGCATCATGTCGCCTCGATGCGGAATGAGTGACCTAATCAAAATCAACAACACGACAACGACGACGATTCACTCAACGCGCCGATATGCGTTCTTCAACGGCCAGCCGCGATGGATTCGAGCCTCGACGTTAACCTACGCTCTCTCACCAGACTACACAATCGAATACCTAACTTCATCGGAAATCCGCAAGGTCGTCCGACGATCGTTCTCCCGGTGGTCCGCAGTGATTCCGCTGAACTTCACGGAGTCCTCCGACTACGAATCATCTGACATACGAATCGGATTCTACCGCGGCGATCACGGCGACGGAGAGGCGTTCGACGGAGTATTAGGAGTTCTGGCGCACGCGTTTTCGCCGGAAAACGGAAGGCTCCACCTGGACGCGGCGGAGCGGTGGGCGGTTGATTTCGAGAAGGAGAAATCGAAAGTTGCAGTGGATTTGGAATCGGTTGTGACGCATGAGATAGGGCACGTTCTTGGACTGGCTCACTCTGCGGTGAAGGAAGCAGTGATGTATCCGAGTTTGAGTCCGCGAGGGAAGAAGGTGGACCTCAGGATCGATGACGTGGAAGGAGTTCAGACTTTGTATGGTTCAAATCCGAATTTCAAATTGAAATCGTTTTTGGAATCTGAAAAGTCTATCAACAGTACTGGATCATCAATCCACAGTCATTTCTTCTTCTTCTTCTTTTACTGGTTCATTTTCGTTTTTCTGTGTGTATGAAATTTGTCATTTAATTTAGGGCTTTTTTTTTCCTTCGTTTTTTCATTTTGTTACAAGTGTATGTATTGGAAAGTTACACGACAAAGGAATGAAAAGGAAATGAAAAGGTACCATTCTTTTACTT

mRNA sequence

GTTTGTTTACCAAACATTAACCAAAATAAAATTTGACTGAAAAAAAAAAACCAAAATAAAACAACATAGCAAAGTCAGAATCTACAATATGAAGGCAAGCACGCCAGAATCTTCTTAACTTTCCCATCATTCTCTGTTTTCTCTTCACCTTCCTCAAATCCAATTTCTAAGAACAGTTAATTAATTAAAGTAATTCATCAATTTATTTTTTTTAGATTCTAAAAACAAAATACTAATAAATAAATCACAAAATCGATTGGTGATAAAGCTTTTTTCCTTTCGATTTCTCTATTGTCCGATAACTGCCAAATAATATCTCCATGTTTCCGTTTTTCCGTTATTACCTCTTCTTCTTCTACCTTCTCTCCTCTGTTTTTCTCTTCTCCTCTGTTTTTCCCGCCAGAACTTTGCCGGACTTCACGACGGCGACTGACACAGACAACAACTACGCCTGGAGAAACTTCGCTCGGTTCCTCGATGCCGGAAAGGGCAGCGAAGTGACCGGCATGTCGGAGCTGAAGAAGTATTTGAATCGATTCGGTTACCTTCCGATTCCTCCAGAGAATAGCTTCTCCGATTGCTTCGACGACCAATTCGTATCGGCGTTGATTCTGTACCAGAAGCGCTTAGGTTTATCAGTCACCGGAAAGCTCGATTCTGAAACGATCGCGAGCATCATGTCGCCTCGATGCGGAATGAGTGACCTAATCAAAATCAACAACACGACAACGACGACGATTCACTCAACGCGCCGATATGCGTTCTTCAACGGCCAGCCGCGATGGATTCGAGCCTCGACGTTAACCTACGCTCTCTCACCAGACTACACAATCGAATACCTAACTTCATCGGAAATCCGCAAGGTCGTCCGACGATCGTTCTCCCGGTGGTCCGCAGTGATTCCGCTGAACTTCACGGAGTCCTCCGACTACGAATCATCTGACATACGAATCGGATTCTACCGCGGCGATCACGGCGACGGAGAGGCGTTCGACGGAGTATTAGGAGTTCTGGCGCACGCGTTTTCGCCGGAAAACGGAAGGCTCCACCTGGACGCGGCGGAGCGGTGGGCGGTTGATTTCGAGAAGGAGAAATCGAAAGTTGCAGTGGATTTGGAATCGGTTGTGACGCATGAGATAGGGCACGTTCTTGGACTGGCTCACTCTGCGGTGAAGGAAGCAGTGATGTATCCGAGTTTGAGTCCGCGAGGGAAGAAGGTGGACCTCAGGATCGATGACGTGGAAGGAGTTCAGACTTTGTATGGTTCAAATCCGAATTTCAAATTGAAATCGTTTTTGGAATCTGAAAAGTCTATCAACAGTACTGGATCATCAATCCACAGTCATTTCTTCTTCTTCTTCTTTTACTGGTTCATTTTCGTTTTTCTGTGTGTATGAAATTTGTCATTTAATTTAGGGCTTTTTTTTTCCTTCGTTTTTTCATTTTGTTACAAGTGTATGTATTGGAAAGTTACACGACAAAGGAATGAAAAGGAAATGAAAAGGTACCATTCTTTTACTT

Coding sequence (CDS)

ATGTTTCCGTTTTTCCGTTATTACCTCTTCTTCTTCTACCTTCTCTCCTCTGTTTTTCTCTTCTCCTCTGTTTTTCCCGCCAGAACTTTGCCGGACTTCACGACGGCGACTGACACAGACAACAACTACGCCTGGAGAAACTTCGCTCGGTTCCTCGATGCCGGAAAGGGCAGCGAAGTGACCGGCATGTCGGAGCTGAAGAAGTATTTGAATCGATTCGGTTACCTTCCGATTCCTCCAGAGAATAGCTTCTCCGATTGCTTCGACGACCAATTCGTATCGGCGTTGATTCTGTACCAGAAGCGCTTAGGTTTATCAGTCACCGGAAAGCTCGATTCTGAAACGATCGCGAGCATCATGTCGCCTCGATGCGGAATGAGTGACCTAATCAAAATCAACAACACGACAACGACGACGATTCACTCAACGCGCCGATATGCGTTCTTCAACGGCCAGCCGCGATGGATTCGAGCCTCGACGTTAACCTACGCTCTCTCACCAGACTACACAATCGAATACCTAACTTCATCGGAAATCCGCAAGGTCGTCCGACGATCGTTCTCCCGGTGGTCCGCAGTGATTCCGCTGAACTTCACGGAGTCCTCCGACTACGAATCATCTGACATACGAATCGGATTCTACCGCGGCGATCACGGCGACGGAGAGGCGTTCGACGGAGTATTAGGAGTTCTGGCGCACGCGTTTTCGCCGGAAAACGGAAGGCTCCACCTGGACGCGGCGGAGCGGTGGGCGGTTGATTTCGAGAAGGAGAAATCGAAAGTTGCAGTGGATTTGGAATCGGTTGTGACGCATGAGATAGGGCACGTTCTTGGACTGGCTCACTCTGCGGTGAAGGAAGCAGTGATGTATCCGAGTTTGAGTCCGCGAGGGAAGAAGGTGGACCTCAGGATCGATGACGTGGAAGGAGTTCAGACTTTGTATGGTTCAAATCCGAATTTCAAATTGAAATCGTTTTTGGAATCTGAAAAGTCTATCAACAGTACTGGATCATCAATCCACAGTCATTTCTTCTTCTTCTTCTTTTACTGGTTCATTTTCGTTTTTCTGTGTGTATGA

Protein sequence

MFPFFRYYLFFFYLLSSVFLFSSVFPARTLPDFTTATDTDNNYAWRNFARFLDAGKGSEVTGMSELKKYLNRFGYLPIPPENSFSDCFDDQFVSALILYQKRLGLSVTGKLDSETIASIMSPRCGMSDLIKINNTTTTTIHSTRRYAFFNGQPRWIRASTLTYALSPDYTIEYLTSSEIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEAFDGVLGVLAHAFSPENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRGKKVDLRIDDVEGVQTLYGSNPNFKLKSFLESEKSINSTGSSIHSHFFFFFFYWFIFVFLCV
Homology
BLAST of Tan0007056 vs. ExPASy Swiss-Prot
Match: O23507 (Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 3.3e-91
Identity = 176/325 (54.15%), Postives = 229/325 (70.46%), Query Frame = 0

Query: 18  VFLFSSVFPARTLPDF--TTATDTD----NNYAWRNFARFLDAGKGSEVTGMSELKKYLN 77
           +F F   F AR  P+   +TA  T     +N  W +F+R +D   GS V+G+SELK+YL+
Sbjct: 19  LFCFPYRFGARNTPEAEQSTAKATQIIHVSNSTWHDFSRLVDVQIGSHVSGVSELKRYLH 78

Query: 78  RFGYLPIPPENSFSDCFDDQFVSALILYQKRLGLSVTGKLDSETIASIMSPRCGMSDL-I 137
           RFGY+    E  FSD FD    SA+ LYQ+ LGL +TG+LD+ T+  +  PRCG+SD  +
Sbjct: 79  RFGYVNDGSE-IFSDVFDGPLESAISLYQENLGLPITGRLDTSTVTLMSLPRCGVSDTHM 138

Query: 138 KINNTTTTTIHSTRRYAFFNGQPRWIRASTLTYALSPDYTIEYLTSSEIRKVVRRSFSRW 197
            INN     +H+T  Y +FNG+P+W R  TLTYA+S  + ++YLTS +++ V RR+FS+W
Sbjct: 139 TINN---DFLHTTAHYTYFNGKPKWNR-DTLTYAISKTHKLDYLTSEDVKTVFRRAFSQW 198

Query: 198 SAVIPLNFTESSDYESSDIRIGFYRGDHGDGEAFDGVLGVLAHAFSPENGRLHLDAAERW 257
           S+VIP++F E  D+ ++D++IGFY GDHGDG  FDGVLG LAHAF+PENGRLHLDAAE W
Sbjct: 199 SSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETW 258

Query: 258 AVDFE-KEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRGKKVDLRIDDVEG 317
            VD + K  S+VAVDLESV THEIGH+LGL HS+ + AVMYPSL PR KKVDL +DDV G
Sbjct: 259 IVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAG 318

Query: 318 VQTLYGSNPNFKLKSFLESEKSINS 335
           V  LYG NP  +L S  +SE SI +
Sbjct: 319 VLKLYGPNPKLRLDSLTQSEDSIKN 338

BLAST of Tan0007056 vs. ExPASy Swiss-Prot
Match: Q8GWW6 (Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana OX=3702 GN=4MMP PE=1 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 1.2e-85
Identity = 171/336 (50.89%), Postives = 218/336 (64.88%), Query Frame = 0

Query: 9   LFFFYLLSSVFLFSSVFPARTLPDFTTATDTDNNYAWRNFARFLDAGKGSEVTGMSELKK 68
           +F F+LL        +  AR    FTT    D                      + E+K+
Sbjct: 16  IFSFFLLYLNLHNQQIIEARNPSQFTTNPSPD--------------------VSIPEIKR 75

Query: 69  YLNRFGYLPIPPENSFSDCFDDQFVSALILYQKRLGLSVTGKLDSETIASIMSPRCGMSD 128
           +L ++GYL   P+N  SD  D  F  AL+ YQK LGL +TGK DS+T++ I+ PRCG  D
Sbjct: 76  HLQQYGYL---PQNKESD--DVSFEQALVRYQKNLGLPITGKPDSDTLSQILLPRCGFPD 135

Query: 129 LIKINNTTTTTIHSTRRYAFFNGQPRWIR--ASTLTYALSPDYTIEYLTSSEIRKVVRRS 188
            ++     T   H+ ++Y +F G+PRW R     LTYA S +    YL  ++IR+V RR+
Sbjct: 136 DVE---PKTAPFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLTPYLAPTDIRRVFRRA 195

Query: 189 FSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEAFDGVLGVLAHAFSPENGRLHLDA 248
           F +W++VIP++F E+ DY  +DI+IGF+ GDHGDGE FDGVLGVLAH FSPENGRLHLD 
Sbjct: 196 FGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFSPENGRLHLDK 255

Query: 249 AERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRGKKVDLRIDD 308
           AE WAVDF++EKS VAVDLESV  HEIGHVLGL HS+VK+A MYP+L PR KKV+L +DD
Sbjct: 256 AETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKKVNLNMDD 315

Query: 309 VEGVQTLYGSNPNFKLKSFLESEKSIN-STGSSIHS 342
           V GVQ+LYG+NPNF L S L SE S N + GS I S
Sbjct: 316 VVGVQSLYGTNPNFTLNSLLASETSTNLADGSRIRS 323

BLAST of Tan0007056 vs. ExPASy Swiss-Prot
Match: O04529 (Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 5.0e-71
Identity = 151/335 (45.07%), Postives = 197/335 (58.81%), Query Frame = 0

Query: 6   RYYLFFFYLLSSVFLFSSVFPARTLPDFTTATDTDNNYA---WRNFARFLDAGKGSEVTG 65
           R+ +F F    S+FL  S   A   P+ T    +  N     W  F+ F     G  V G
Sbjct: 2   RFCVFGFL---SLFLIVSPASAWFFPNSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDG 61

Query: 66  MSELKKYLNRFGYLPIPPENSFSDCFDDQFVSALILYQKRLGLSVTGKLDSETIASIMSP 125
           +  +KKY  RFGY+P     +F+D FDD   +A+ LYQ    L+VTG+LD+ TI  I+ P
Sbjct: 62  LYRIKKYFQRFGYIPETFSGNFTDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIP 121

Query: 126 RCGMSDLIKIN-------------NTTTTTIHSTRRYAFFNGQPRWIR-ASTLTYALSPD 185
           RCG  D++                N + T +H+ +RY  F G+PRW R    LTYA  P 
Sbjct: 122 RCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPK 181

Query: 186 YTIEYLTSSEIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEAFDGVL 245
             +    + E++ V  R+F RWS V  LNFT S  + +SDI IGFY GDHGDGE FDGVL
Sbjct: 182 NPL----TEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVL 241

Query: 246 GVLAHAFSPENGRLHLDAAERWAVDFEKE---KSKVAVDLESVVTHEIGHVLGLAHSAVK 305
           G LAHAFSP +G+ HLDA E W V  + +       AVDLESV  HEIGH+LGL HS+V+
Sbjct: 242 GTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVE 301

Query: 306 EAVMYPSLSPRGKKVDLRIDDVEGVQTLYGSNPNF 321
           E++MYP+++   +KVDL  DDVEG+Q LYG+NPNF
Sbjct: 302 ESIMYPTITTGKRKVDLTNDDVEGIQYLYGANPNF 329

BLAST of Tan0007056 vs. ExPASy Swiss-Prot
Match: Q9ZUJ5 (Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 6.3e-66
Identity = 142/341 (41.64%), Postives = 199/341 (58.36%), Query Frame = 0

Query: 9   LFFFYLLS--SVFLFSSVFPARTLPDFTTATDTDNNYAWRNFARFLDAGKGSEVTGMSEL 68
           L FF+ ++  S   +++V    ++P       T N  AW  F++      G  + G+S+L
Sbjct: 9   LIFFFTVNPISAKFYTNV---SSIPPLQFLNATQN--AWETFSKLAGCHIGENINGLSKL 68

Query: 69  KKYLNRFGYLPIPPENSFSDCFDDQFVSALILYQKRLGLSVTGKLDSETIASIMSPRCGM 128
           K+Y  RFGY  I    + +D FDD   SA+  YQK   L VTGKLDS T+  I+ PRCG 
Sbjct: 69  KQYFRRFGY--ITTTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCGN 128

Query: 129 SDLIK--INNTTTTTIHSTRRYAFFNGQPRW-IRASTLTYALSPDYTIEYLTSSEIRKVV 188
            DLI           + +T +Y+FF G+PRW  R   LTYA +P   +    + E+++V 
Sbjct: 129 PDLIDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNL----TDEVKRVF 188

Query: 189 RRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEAFDGVLGVLAHAFSPENGRLH 248
            R+F+RW+ V PLNFT S     +DI IGF+ G+HGDGE FDG +G LAHA SP  G LH
Sbjct: 189 SRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASSPPTGMLH 248

Query: 249 LDAAERWAVDFEKEKSKV-----AVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRGK 308
           LD  E W +   +   ++      VDLESV  HEIGH+LGL HS+V++A+M+P++S   +
Sbjct: 249 LDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFPAISGGDR 308

Query: 309 KVDLRIDDVEGVQTLYGSNPNFKLKSFLESEKSINSTGSSI 340
           KV+L  DD+EG+Q LYG NPN        S +S ++ G S+
Sbjct: 309 KVELAKDDIEGIQHLYGGNPNGDGGGSKPSRESQSTGGDSV 338

BLAST of Tan0007056 vs. ExPASy Swiss-Prot
Match: P29136 (Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2)

HSP 1 Score: 237.3 bits (604), Expect = 2.7e-61
Identity = 131/306 (42.81%), Postives = 178/306 (58.17%), Query Frame = 0

Query: 18  VFLFSSVFPARTLPDFTTATDTDNNYAWRNFA--RFLDAGKGSEVTGMSELKKYLNRFGY 77
           V L +  F A +LP    +      YAW   A  +F     G    G+S +K Y +  GY
Sbjct: 11  VALATLYFLATSLP----SVSAHGPYAWDGEATYKFTTYHPGQNYKGLSNVKNYFHHLGY 70

Query: 78  LPIPPENSFSDCFDDQFVSALILYQKRLGLSVTGKLDSETIASIMSPRCGMSDLIKINNT 137
           +P  P   F D FDD  VSA+  YQK   L+VTGK D  T+  IM+PRCG+ D+I IN  
Sbjct: 71  IPNAPH--FDDNFDDTLVSAIKTYQKNYNLNVTGKFDINTLKQIMTPRCGVPDII-INTN 130

Query: 138 TTTTIHSTRRYAFFNGQPRWIRAST-LTYALSPDYTIEYLTSSEIRKVVRRSFSRWSAVI 197
            TT+      Y FF   PRW   +T LTYA SP+  ++       +  + R+FS+W+ V+
Sbjct: 131 KTTSFGMISDYTFFKDMPRWQAGTTQLTYAFSPEPRLD----DTFKSAIARAFSKWTPVV 190

Query: 198 PLNFTESSDYESSDIRIGFYRGDHGDGEAFDGVLGVLAHAFSPENGRLHLDAAERWAVDF 257
            + F E++ YE+++I+I F   +HGD   FDG  G+L HAF+P +GR H DA E W    
Sbjct: 191 NIAFQETTSYETANIKILFASKNHGDPYPFDGPGGILGHAFAPTDGRCHFDADEYWVASG 250

Query: 258 EKEKSKV--AVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRGKKVDLRIDDVEGVQT 317
           +  KS V  A DLESV  HEIGH+LGL HS+   A+MYPS+ PR +KV+L  DD++G++ 
Sbjct: 251 DVTKSPVTSAFDLESVAVHEIGHLLGLGHSSDLRAIMYPSIPPRTRKVNLAQDDIDGIRK 305

Query: 318 LYGSNP 319
           LYG NP
Sbjct: 311 LYGINP 305

BLAST of Tan0007056 vs. NCBI nr
Match: XP_023532756.1 (metalloendoproteinase 4-MMP-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 626.3 bits (1614), Expect = 1.6e-175
Identity = 322/358 (89.94%), Postives = 335/358 (93.58%), Query Frame = 0

Query: 1   MFPFFRYYLFFFYLLSSVFLFSSVFPARTLPDFTTAT---DTDNNYAWRNFARFLDAGKG 60
           MFPFFRYYLFFFYLLSSVFLFSSVFPA T  DFTTAT   D  NNYAWRNFARFLDAG+G
Sbjct: 1   MFPFFRYYLFFFYLLSSVFLFSSVFPATTPLDFTTATAAFDASNNYAWRNFARFLDAGEG 60

Query: 61  SEVTGMSELKKYLNRFGYLPIPPENSFSDCFDDQFVSALILYQKRLGLSVTGKLDSETIA 120
           SEVTGMSELKKYLNRFGYLP+ PEN+FSD  DDQFVSALILYQKRLGLSVTGKLDSETIA
Sbjct: 61  SEVTGMSELKKYLNRFGYLPVSPENNFSDFVDDQFVSALILYQKRLGLSVTGKLDSETIA 120

Query: 121 SIMSPRCGMSDLIKINNTTTTTIHSTRRYAFFNGQPRWIRASTLTYALSPDYTIEYLTSS 180
           SIMSPRCGMSDLIKIN+ TT+TIHSTRRYAFFNGQPRWIRASTLTYALSPDYTIEYLTSS
Sbjct: 121 SIMSPRCGMSDLIKINDATTSTIHSTRRYAFFNGQPRWIRASTLTYALSPDYTIEYLTSS 180

Query: 181 EIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEAFDGVLGVLAHAFSP 240
           EIR VVRRSFSRWSAVIPLNFTES DYESSDIRIGFY+GDHGDGEAFDGVLGVLAHAFSP
Sbjct: 181 EIRNVVRRSFSRWSAVIPLNFTESLDYESSDIRIGFYQGDHGDGEAFDGVLGVLAHAFSP 240

Query: 241 ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG 300
           ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG
Sbjct: 241 ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG 300

Query: 301 KKVDLRIDDVEGVQTLYGSNPNFKLKSFLESEKSINSTGSSIHSHFFFFFFYWFIFVF 356
           KKV+LR+DDVEGVQ LYGSNPNFKL SFLESEKS N+  S+  + FFFFFF +FIFV+
Sbjct: 301 KKVELRMDDVEGVQALYGSNPNFKLDSFLESEKSTNTGSSNTTNFFFFFFFSFFIFVW 358

BLAST of Tan0007056 vs. NCBI nr
Match: KAG7036120.1 (Metalloendoproteinase 1-MMP, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 623.6 bits (1607), Expect = 1.0e-174
Identity = 323/361 (89.47%), Postives = 335/361 (92.80%), Query Frame = 0

Query: 1   MFPFFRYYLFFFYLLSSVFLFSSVFPARTLPDFTTAT---DTDNNYAWRNFARFLDAGKG 60
           MFPFFRYYLFFFYLLSSVFLFSSVFPA T  DFTTAT   D  NNY WRNFARFLDAGKG
Sbjct: 1   MFPFFRYYLFFFYLLSSVFLFSSVFPATTPLDFTTATAAFDVSNNYVWRNFARFLDAGKG 60

Query: 61  SEVTGMSELKKYLNRFGYLPIPPENSFSDCFDDQFVSALILYQKRLGLSVTGKLDSETIA 120
           SEVTGMSELKKYLNRFGYLP+ PEN+FSD  DDQFVSALILYQKRLGLSVTGKLDSETIA
Sbjct: 61  SEVTGMSELKKYLNRFGYLPVSPENNFSDFVDDQFVSALILYQKRLGLSVTGKLDSETIA 120

Query: 121 SIMSPRCGMSDLIKINNTTTTTIHSTRRYAFFNGQPRWIRASTLTYALSPDYTIEYLTSS 180
           SIMSPRCGMSDLIKIN+ TT+TIHSTRRYAFFNGQPRWIR STLTYALSPDYTIEYLTSS
Sbjct: 121 SIMSPRCGMSDLIKINDATTSTIHSTRRYAFFNGQPRWIRGSTLTYALSPDYTIEYLTSS 180

Query: 181 EIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEAFDGVLGVLAHAFSP 240
           EIR VVRRSFSRWSAVIPLNFTES DYESSDIRIGFY+GDHGDGEAFDGVLGVLAHAFSP
Sbjct: 181 EIRNVVRRSFSRWSAVIPLNFTESLDYESSDIRIGFYQGDHGDGEAFDGVLGVLAHAFSP 240

Query: 241 ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG 300
           ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG
Sbjct: 241 ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG 300

Query: 301 KKVDLRIDDVEGVQTLYGSNPNFKLKSFLESEKSINSTGSSIHSHFFFFFFYWFIFVFLC 359
           KKV+LR+DDVEGVQ LYGSNPNFKL SFLESEKS N TGSS  ++FF FFF+ F+F F+ 
Sbjct: 301 KKVELRMDDVEGVQALYGSNPNFKLDSFLESEKSTN-TGSSNTTNFFSFFFFSFLFFFIF 360

BLAST of Tan0007056 vs. NCBI nr
Match: XP_022957642.1 (metalloendoproteinase 4-MMP-like [Cucurbita moschata])

HSP 1 Score: 620.9 bits (1600), Expect = 6.7e-174
Identity = 319/359 (88.86%), Postives = 331/359 (92.20%), Query Frame = 0

Query: 1   MFPFFRYYLFFFYLLSSVFLFSSVFPARTLPDFTTAT---DTDNNYAWRNFARFLDAGKG 60
           MFPFFRYYLFFFYLLSSVFLFSSVFPA T  DFTTAT   D  NNY WRNFARFLDAGKG
Sbjct: 1   MFPFFRYYLFFFYLLSSVFLFSSVFPATTPLDFTTATAAFDASNNYVWRNFARFLDAGKG 60

Query: 61  SEVTGMSELKKYLNRFGYLPIPPENSFSDCFDDQFVSALILYQKRLGLSVTGKLDSETIA 120
           SEVTGMSELKKYLNRFGYLP+ PEN+FSD  DDQFVSALILYQKRLGLSVTGKLDSETIA
Sbjct: 61  SEVTGMSELKKYLNRFGYLPVSPENNFSDFVDDQFVSALILYQKRLGLSVTGKLDSETIA 120

Query: 121 SIMSPRCGMSDLIKINNTTTTTIHSTRRYAFFNGQPRWIRASTLTYALSPDYTIEYLTSS 180
           SIMSPRCGMSDLIKIN+ TT+TIHSTRRYAFFNGQPRWIR STLTYALSPDYTIEYLTSS
Sbjct: 121 SIMSPRCGMSDLIKINDATTSTIHSTRRYAFFNGQPRWIRGSTLTYALSPDYTIEYLTSS 180

Query: 181 EIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEAFDGVLGVLAHAFSP 240
           EIR VVRRSFSRWSAVIPLNFTES DYESSDIRIGFY+GDHGDGEAFDGVLGVLAHAFSP
Sbjct: 181 EIRNVVRRSFSRWSAVIPLNFTESLDYESSDIRIGFYQGDHGDGEAFDGVLGVLAHAFSP 240

Query: 241 ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG 300
           ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG
Sbjct: 241 ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG 300

Query: 301 KKVDLRIDDVEGVQTLYGSNPNFKLKSFLESEKSINSTGSSIHSHFFFFFFYWFIFVFL 357
           KKV+LR+DDVEGVQ LYGSNPNFKL SFLESEKS N+  S+  + F FFFF  F F+F+
Sbjct: 301 KKVELRMDDVEGVQALYGSNPNFKLDSFLESEKSTNTGSSNTTNFFSFFFFSLFFFIFV 359

BLAST of Tan0007056 vs. NCBI nr
Match: XP_022996404.1 (metalloendoproteinase 1-MMP-like [Cucurbita maxima])

HSP 1 Score: 615.1 bits (1585), Expect = 3.7e-172
Identity = 319/357 (89.36%), Postives = 329/357 (92.16%), Query Frame = 0

Query: 1   MFPFFRYYLFFFYLLSSVFLFSSVFPARTLPDFTTAT---DTDNNYAWRNFARFLDAGKG 60
           MFPFFRYY+FFFYLLSSVFLFSSVFPA T  DFTTAT   D  NNY WRNFARFLDAGKG
Sbjct: 1   MFPFFRYYIFFFYLLSSVFLFSSVFPATTPLDFTTATAAFDASNNYVWRNFARFLDAGKG 60

Query: 61  SEVTGMSELKKYLNRFGYLPIPPENSFSDCFDDQFVSALILYQKRLGLSVTGKLDSETIA 120
           SEVTGMSELKKYLNRFGYLP+ PEN+FSD  DDQFVSALILYQ+RLGLSVTGKLDSETIA
Sbjct: 61  SEVTGMSELKKYLNRFGYLPVFPENNFSDFVDDQFVSALILYQRRLGLSVTGKLDSETIA 120

Query: 121 SIMSPRCGMSDLIKINNTTTTTIHSTRRYAFFNGQPRWIRASTLTYALSPDYTIEYLTSS 180
           SIMSPRCGMSDLIKIN+ TTTTIHSTRRYAFFNGQPRWIR STLTYALSPDYTIEYLTSS
Sbjct: 121 SIMSPRCGMSDLIKINDATTTTIHSTRRYAFFNGQPRWIRGSTLTYALSPDYTIEYLTSS 180

Query: 181 EIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEAFDGVLGVLAHAFSP 240
           EIR VV RSFSRWSAVIPLNFTESS+YESSDIRIGFY GDHGDGEAFDGVLGVLAHAFSP
Sbjct: 181 EIRNVVGRSFSRWSAVIPLNFTESSNYESSDIRIGFYHGDHGDGEAFDGVLGVLAHAFSP 240

Query: 241 ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG 300
           ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG
Sbjct: 241 ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG 300

Query: 301 KKVDLRIDDVEGVQTLYGSNPNFKLKSFLESEKSINSTGSSIHSHF---FFFFFYWF 352
           KKV+LR+DDVEGVQ LYGSNPNFKL SFLESEKS N TGSS  ++F   FFF F WF
Sbjct: 301 KKVELRMDDVEGVQALYGSNPNFKLDSFLESEKSTN-TGSSNTTNFLSLFFFIFVWF 356

BLAST of Tan0007056 vs. NCBI nr
Match: XP_038875312.1 (metalloendoproteinase 1-MMP [Benincasa hispida])

HSP 1 Score: 613.2 bits (1580), Expect = 1.4e-171
Identity = 312/359 (86.91%), Postives = 331/359 (92.20%), Query Frame = 0

Query: 1   MFPFFRYYLFFFYLLSSVFLFSSVFPARTLPDFTTATDTDNNYAWRNFARFLDAGKGSEV 60
           M PFFRY LFFFY+ SSVFLFS VFPARTLPDFTT  D DNNY WRNFARFLDAGKG+EV
Sbjct: 1   MLPFFRYSLFFFYIFSSVFLFSPVFPARTLPDFTT-LDADNNYGWRNFARFLDAGKGTEV 60

Query: 61  TGMSELKKYLNRFGYLPIPPENSFSDCFDDQFVSALILYQKRLGLSVTGKLDSETIASIM 120
            GMSELKKYLNRFGYLPIPP+N+FSD FDDQF+SALILYQ RLGLSVTGKLDSETIASI+
Sbjct: 61  NGMSELKKYLNRFGYLPIPPQNNFSDFFDDQFLSALILYQNRLGLSVTGKLDSETIASII 120

Query: 121 SPRCGMSDLIKINN---TTTTTIHSTRRYAFFNGQPRWIRASTLTYALSPDYTIEYLTSS 180
           SPRCGMSDLIKINN   TTTTTIHSTRRYAFFNGQPRWIR+STLTYALSPDYTIEYLT S
Sbjct: 121 SPRCGMSDLIKINNNTTTTTTTIHSTRRYAFFNGQPRWIRSSTLTYALSPDYTIEYLTPS 180

Query: 181 EIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEAFDGVLGVLAHAFSP 240
           EIRKV+RRSFSRWSAVIPLNFTESSDYESSDIRIGF+RGDHGDGEAFDGVLGVLAHAFSP
Sbjct: 181 EIRKVIRRSFSRWSAVIPLNFTESSDYESSDIRIGFFRGDHGDGEAFDGVLGVLAHAFSP 240

Query: 241 ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG 300
           ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHS VKE+VMYPSLSPRG
Sbjct: 241 ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSTVKESVMYPSLSPRG 300

Query: 301 KKVDLRIDDVEGVQTLYGSNPNFKLKSFLESEKSINSTGSSIHSHFFFFFFYWFIFVFL 357
           KKVDLRIDDVEG+Q LYG+NPNFKLKSFLESEKSI+S  SS +   FFF   +++ + +
Sbjct: 301 KKVDLRIDDVEGIQVLYGTNPNFKLKSFLESEKSIDSGSSSSNDANFFFLLVFYLLILV 358

BLAST of Tan0007056 vs. ExPASy TrEMBL
Match: A0A6J1GZP3 (metalloendoproteinase 4-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111459118 PE=3 SV=1)

HSP 1 Score: 620.9 bits (1600), Expect = 3.3e-174
Identity = 319/359 (88.86%), Postives = 331/359 (92.20%), Query Frame = 0

Query: 1   MFPFFRYYLFFFYLLSSVFLFSSVFPARTLPDFTTAT---DTDNNYAWRNFARFLDAGKG 60
           MFPFFRYYLFFFYLLSSVFLFSSVFPA T  DFTTAT   D  NNY WRNFARFLDAGKG
Sbjct: 1   MFPFFRYYLFFFYLLSSVFLFSSVFPATTPLDFTTATAAFDASNNYVWRNFARFLDAGKG 60

Query: 61  SEVTGMSELKKYLNRFGYLPIPPENSFSDCFDDQFVSALILYQKRLGLSVTGKLDSETIA 120
           SEVTGMSELKKYLNRFGYLP+ PEN+FSD  DDQFVSALILYQKRLGLSVTGKLDSETIA
Sbjct: 61  SEVTGMSELKKYLNRFGYLPVSPENNFSDFVDDQFVSALILYQKRLGLSVTGKLDSETIA 120

Query: 121 SIMSPRCGMSDLIKINNTTTTTIHSTRRYAFFNGQPRWIRASTLTYALSPDYTIEYLTSS 180
           SIMSPRCGMSDLIKIN+ TT+TIHSTRRYAFFNGQPRWIR STLTYALSPDYTIEYLTSS
Sbjct: 121 SIMSPRCGMSDLIKINDATTSTIHSTRRYAFFNGQPRWIRGSTLTYALSPDYTIEYLTSS 180

Query: 181 EIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEAFDGVLGVLAHAFSP 240
           EIR VVRRSFSRWSAVIPLNFTES DYESSDIRIGFY+GDHGDGEAFDGVLGVLAHAFSP
Sbjct: 181 EIRNVVRRSFSRWSAVIPLNFTESLDYESSDIRIGFYQGDHGDGEAFDGVLGVLAHAFSP 240

Query: 241 ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG 300
           ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG
Sbjct: 241 ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG 300

Query: 301 KKVDLRIDDVEGVQTLYGSNPNFKLKSFLESEKSINSTGSSIHSHFFFFFFYWFIFVFL 357
           KKV+LR+DDVEGVQ LYGSNPNFKL SFLESEKS N+  S+  + F FFFF  F F+F+
Sbjct: 301 KKVELRMDDVEGVQALYGSNPNFKLDSFLESEKSTNTGSSNTTNFFSFFFFSLFFFIFV 359

BLAST of Tan0007056 vs. ExPASy TrEMBL
Match: A0A6J1K8M4 (metalloendoproteinase 1-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111491637 PE=3 SV=1)

HSP 1 Score: 615.1 bits (1585), Expect = 1.8e-172
Identity = 319/357 (89.36%), Postives = 329/357 (92.16%), Query Frame = 0

Query: 1   MFPFFRYYLFFFYLLSSVFLFSSVFPARTLPDFTTAT---DTDNNYAWRNFARFLDAGKG 60
           MFPFFRYY+FFFYLLSSVFLFSSVFPA T  DFTTAT   D  NNY WRNFARFLDAGKG
Sbjct: 1   MFPFFRYYIFFFYLLSSVFLFSSVFPATTPLDFTTATAAFDASNNYVWRNFARFLDAGKG 60

Query: 61  SEVTGMSELKKYLNRFGYLPIPPENSFSDCFDDQFVSALILYQKRLGLSVTGKLDSETIA 120
           SEVTGMSELKKYLNRFGYLP+ PEN+FSD  DDQFVSALILYQ+RLGLSVTGKLDSETIA
Sbjct: 61  SEVTGMSELKKYLNRFGYLPVFPENNFSDFVDDQFVSALILYQRRLGLSVTGKLDSETIA 120

Query: 121 SIMSPRCGMSDLIKINNTTTTTIHSTRRYAFFNGQPRWIRASTLTYALSPDYTIEYLTSS 180
           SIMSPRCGMSDLIKIN+ TTTTIHSTRRYAFFNGQPRWIR STLTYALSPDYTIEYLTSS
Sbjct: 121 SIMSPRCGMSDLIKINDATTTTIHSTRRYAFFNGQPRWIRGSTLTYALSPDYTIEYLTSS 180

Query: 181 EIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEAFDGVLGVLAHAFSP 240
           EIR VV RSFSRWSAVIPLNFTESS+YESSDIRIGFY GDHGDGEAFDGVLGVLAHAFSP
Sbjct: 181 EIRNVVGRSFSRWSAVIPLNFTESSNYESSDIRIGFYHGDHGDGEAFDGVLGVLAHAFSP 240

Query: 241 ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG 300
           ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG
Sbjct: 241 ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG 300

Query: 301 KKVDLRIDDVEGVQTLYGSNPNFKLKSFLESEKSINSTGSSIHSHF---FFFFFYWF 352
           KKV+LR+DDVEGVQ LYGSNPNFKL SFLESEKS N TGSS  ++F   FFF F WF
Sbjct: 301 KKVELRMDDVEGVQALYGSNPNFKLDSFLESEKSTN-TGSSNTTNFLSLFFFIFVWF 356

BLAST of Tan0007056 vs. ExPASy TrEMBL
Match: A0A6J1I297 (metalloendoproteinase 1-MMP OS=Cucurbita maxima OX=3661 GN=LOC111468956 PE=3 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 2.2e-170
Identity = 319/369 (86.45%), Postives = 333/369 (90.24%), Query Frame = 0

Query: 1   MFPFFRYYLFFFYLLSSVFLFSSVFPARTLPDFTTAT---DTDNNYAWRNFARFLDAGKG 60
           MFPFFRYYL FFYLL       SVFPART  DFT AT   D DNNYAWRNFARFLDAGKG
Sbjct: 1   MFPFFRYYLCFFYLL------YSVFPARTFADFTAATASLDGDNNYAWRNFARFLDAGKG 60

Query: 61  SEVTGMSELKKYLNRFGYLPIPPENSFSDCFDDQFVSALILYQKRLGLSVTGKLDSETIA 120
            EV GMSELKKYLNRFGYLPIPPEN+FSD FDDQFVSALILYQKRLGLSVTGKLDSETIA
Sbjct: 61  REVNGMSELKKYLNRFGYLPIPPENNFSDSFDDQFVSALILYQKRLGLSVTGKLDSETIA 120

Query: 121 SIMSPRCGMSDLIKINNTTTTTIHSTRRYAFFNGQPRWIRASTLTYALSPDYTIEYLTSS 180
           SIMSPRCGMSDLIKIN++ +TTI STRRYAFFNGQPRWIR+STLTYALSPDYTIEYLTSS
Sbjct: 121 SIMSPRCGMSDLIKINHSVSTTIRSTRRYAFFNGQPRWIRSSTLTYALSPDYTIEYLTSS 180

Query: 181 EIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEAFDGVLGVLAHAFSP 240
           EIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGF+RGDHGDGE+FDGVLG+LAHAFSP
Sbjct: 181 EIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFFRGDHGDGESFDGVLGILAHAFSP 240

Query: 241 ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG 300
           ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG
Sbjct: 241 ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG 300

Query: 301 KKVDLRIDDVEGVQTLYGSNPNFKLKSFLESEKSIN-----STGSSIHSHFFFFFFY--- 357
           KKVDLRIDDVEGVQ LYGSNPNFKLKSFLESEKSIN     S+ SS +++FFFF F+   
Sbjct: 301 KKVDLRIDDVEGVQALYGSNPNFKLKSFLESEKSINNNSGSSSSSSNNANFFFFLFFPSI 360

BLAST of Tan0007056 vs. ExPASy TrEMBL
Match: A0A6J1E2L9 (metalloendoproteinase 1-MMP OS=Cucurbita moschata OX=3662 GN=LOC111430227 PE=3 SV=1)

HSP 1 Score: 601.7 bits (1550), Expect = 2.0e-168
Identity = 316/368 (85.87%), Postives = 330/368 (89.67%), Query Frame = 0

Query: 1   MFPFFRYYLFFFYLLSSVFLFSSVFPARTLPDFTTAT---DTDNNYAWRNFARFLDAGKG 60
           MFPFFRYYL FFYLL       SVFPA T  DFT AT   D DNNYAWRNFARFLDAGKG
Sbjct: 1   MFPFFRYYLCFFYLL------YSVFPATTFADFTAATASLDGDNNYAWRNFARFLDAGKG 60

Query: 61  SEVTGMSELKKYLNRFGYLPIPPENSFSDCFDDQFVSALILYQKRLGLSVTGKLDSETIA 120
            EV GMSELKKYLNRFGYLPIPPEN+FSD FDDQFVSALILYQKRLGLSVTGKLDSETIA
Sbjct: 61  REVNGMSELKKYLNRFGYLPIPPENNFSDSFDDQFVSALILYQKRLGLSVTGKLDSETIA 120

Query: 121 SIMSPRCGMSDLIKINNTTTTTIHSTRRYAFFNGQPRWIRASTLTYALSPDYTIEYLTSS 180
           SIMSPRCGMSDLIKIN++ +TTI STRRYAFFNGQPRWIR+STLTYALSPDYTIEYLTSS
Sbjct: 121 SIMSPRCGMSDLIKINHSVSTTIRSTRRYAFFNGQPRWIRSSTLTYALSPDYTIEYLTSS 180

Query: 181 EIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEAFDGVLGVLAHAFSP 240
           EIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGF+RGDHGDGE+FDGVLG+LAHAFSP
Sbjct: 181 EIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFFRGDHGDGESFDGVLGILAHAFSP 240

Query: 241 ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG 300
           ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG
Sbjct: 241 ENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRG 300

Query: 301 KKVDLRIDDVEGVQTLYGSNPNFKLKSFLESEKSIN----STGSSIHSHFFFFFFY---- 357
           KKVDLRIDDVEGVQ LYGSNPNFKLKS LESEKSIN    S+ SS +++FFF  F+    
Sbjct: 301 KKVDLRIDDVEGVQALYGSNPNFKLKSLLESEKSINNNSGSSSSSNNANFFFLLFFPSIF 360

BLAST of Tan0007056 vs. ExPASy TrEMBL
Match: A0A0A0KRA5 (ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G162050 PE=3 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 5.2e-164
Identity = 306/371 (82.48%), Postives = 326/371 (87.87%), Query Frame = 0

Query: 1   MFPFFRYY-----LFFFYLLSSVFLFSSVFPARTLPDFTTATDTDNN-----YAWRNFAR 60
           MFPFFRYY     LFFF LLSSVFLFS VFPA T PDF+T T  +NN     YAWR FAR
Sbjct: 1   MFPFFRYYPSFFFLFFFNLLSSVFLFSPVFPATTFPDFSTLTPDNNNNNHDYYAWRGFAR 60

Query: 61  FLDAGKGSEVTGMSELKKYLNRFGYLPIPPENSFSDCFDDQFVSALILYQKRLGLSVTGK 120
           FLDAGKG+EV G+SELKKYLNRFGYLP+PP+N+FSD FDDQFVSAL LYQ RLGLSVTGK
Sbjct: 61  FLDAGKGTEVNGISELKKYLNRFGYLPLPPQNNFSDIFDDQFVSALSLYQNRLGLSVTGK 120

Query: 121 LDSETIASIMSPRCGMSDLIKINNTTTTTIHSTRRYAFFNGQPRWIRASTLTYALSPDYT 180
           LDSETIA+IMSPRCG+SDL K NN   +TIHSTRRYAFFNGQPRWIRASTLTYALSPDYT
Sbjct: 121 LDSETIATIMSPRCGISDLTKFNN-NGSTIHSTRRYAFFNGQPRWIRASTLTYALSPDYT 180

Query: 181 IEYLTSSEIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEAFDGVLGV 240
           I+YLT SEIRKVVRRSFSRWSAVIPLNFTESSDY+SSDIRIGFYRGDHGDGEAFDGVLGV
Sbjct: 181 IDYLTPSEIRKVVRRSFSRWSAVIPLNFTESSDYDSSDIRIGFYRGDHGDGEAFDGVLGV 240

Query: 241 LAHAFSPENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMY 300
           LAHAFSPENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKE+VMY
Sbjct: 241 LAHAFSPENGRLHLDAAERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKESVMY 300

Query: 301 PSLSPRGKKVDLRIDDVEGVQTLYGSNPNFKLKSFLESEKSINSTGSSIHSH------FF 356
           PSLSPRGKKVDLRIDDVEG+Q LYG+NPNFKL+SFLESEKS+N+ GSS  S        F
Sbjct: 301 PSLSPRGKKVDLRIDDVEGIQVLYGTNPNFKLESFLESEKSMNNNGSSSSSSNNNSKLLF 360

BLAST of Tan0007056 vs. TAIR 10
Match: AT4G16640.1 (Matrixin family protein )

HSP 1 Score: 336.7 bits (862), Expect = 2.4e-92
Identity = 176/325 (54.15%), Postives = 229/325 (70.46%), Query Frame = 0

Query: 18  VFLFSSVFPARTLPDF--TTATDTD----NNYAWRNFARFLDAGKGSEVTGMSELKKYLN 77
           +F F   F AR  P+   +TA  T     +N  W +F+R +D   GS V+G+SELK+YL+
Sbjct: 19  LFCFPYRFGARNTPEAEQSTAKATQIIHVSNSTWHDFSRLVDVQIGSHVSGVSELKRYLH 78

Query: 78  RFGYLPIPPENSFSDCFDDQFVSALILYQKRLGLSVTGKLDSETIASIMSPRCGMSDL-I 137
           RFGY+    E  FSD FD    SA+ LYQ+ LGL +TG+LD+ T+  +  PRCG+SD  +
Sbjct: 79  RFGYVNDGSE-IFSDVFDGPLESAISLYQENLGLPITGRLDTSTVTLMSLPRCGVSDTHM 138

Query: 138 KINNTTTTTIHSTRRYAFFNGQPRWIRASTLTYALSPDYTIEYLTSSEIRKVVRRSFSRW 197
            INN     +H+T  Y +FNG+P+W R  TLTYA+S  + ++YLTS +++ V RR+FS+W
Sbjct: 139 TINN---DFLHTTAHYTYFNGKPKWNR-DTLTYAISKTHKLDYLTSEDVKTVFRRAFSQW 198

Query: 198 SAVIPLNFTESSDYESSDIRIGFYRGDHGDGEAFDGVLGVLAHAFSPENGRLHLDAAERW 257
           S+VIP++F E  D+ ++D++IGFY GDHGDG  FDGVLG LAHAF+PENGRLHLDAAE W
Sbjct: 199 SSVIPVSFEEVDDFTTADLKIGFYAGDHGDGLPFDGVLGTLAHAFAPENGRLHLDAAETW 258

Query: 258 AVDFE-KEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRGKKVDLRIDDVEG 317
            VD + K  S+VAVDLESV THEIGH+LGL HS+ + AVMYPSL PR KKVDL +DDV G
Sbjct: 259 IVDDDLKGSSEVAVDLESVATHEIGHLLGLGHSSQESAVMYPSLRPRTKKVDLTVDDVAG 318

Query: 318 VQTLYGSNPNFKLKSFLESEKSINS 335
           V  LYG NP  +L S  +SE SI +
Sbjct: 319 VLKLYGPNPKLRLDSLTQSEDSIKN 338

BLAST of Tan0007056 vs. TAIR 10
Match: AT2G45040.1 (Matrixin family protein )

HSP 1 Score: 318.2 bits (814), Expect = 8.7e-87
Identity = 171/336 (50.89%), Postives = 218/336 (64.88%), Query Frame = 0

Query: 9   LFFFYLLSSVFLFSSVFPARTLPDFTTATDTDNNYAWRNFARFLDAGKGSEVTGMSELKK 68
           +F F+LL        +  AR    FTT    D                      + E+K+
Sbjct: 16  IFSFFLLYLNLHNQQIIEARNPSQFTTNPSPD--------------------VSIPEIKR 75

Query: 69  YLNRFGYLPIPPENSFSDCFDDQFVSALILYQKRLGLSVTGKLDSETIASIMSPRCGMSD 128
           +L ++GYL   P+N  SD  D  F  AL+ YQK LGL +TGK DS+T++ I+ PRCG  D
Sbjct: 76  HLQQYGYL---PQNKESD--DVSFEQALVRYQKNLGLPITGKPDSDTLSQILLPRCGFPD 135

Query: 129 LIKINNTTTTTIHSTRRYAFFNGQPRWIR--ASTLTYALSPDYTIEYLTSSEIRKVVRRS 188
            ++     T   H+ ++Y +F G+PRW R     LTYA S +    YL  ++IR+V RR+
Sbjct: 136 DVE---PKTAPFHTGKKYVYFPGRPRWTRDVPLKLTYAFSQENLTPYLAPTDIRRVFRRA 195

Query: 189 FSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEAFDGVLGVLAHAFSPENGRLHLDA 248
           F +W++VIP++F E+ DY  +DI+IGF+ GDHGDGE FDGVLGVLAH FSPENGRLHLD 
Sbjct: 196 FGKWASVIPVSFIETEDYVIADIKIGFFNGDHGDGEPFDGVLGVLAHTFSPENGRLHLDK 255

Query: 249 AERWAVDFEKEKSKVAVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRGKKVDLRIDD 308
           AE WAVDF++EKS VAVDLESV  HEIGHVLGL HS+VK+A MYP+L PR KKV+L +DD
Sbjct: 256 AETWAVDFDEEKSSVAVDLESVAVHEIGHVLGLGHSSVKDAAMYPTLKPRSKKVNLNMDD 315

Query: 309 VEGVQTLYGSNPNFKLKSFLESEKSIN-STGSSIHS 342
           V GVQ+LYG+NPNF L S L SE S N + GS I S
Sbjct: 316 VVGVQSLYGTNPNFTLNSLLASETSTNLADGSRIRS 323

BLAST of Tan0007056 vs. TAIR 10
Match: AT1G70170.1 (matrix metalloproteinase )

HSP 1 Score: 269.6 bits (688), Expect = 3.5e-72
Identity = 151/335 (45.07%), Postives = 197/335 (58.81%), Query Frame = 0

Query: 6   RYYLFFFYLLSSVFLFSSVFPARTLPDFTTATDTDNNYA---WRNFARFLDAGKGSEVTG 65
           R+ +F F    S+FL  S   A   P+ T    +  N     W  F+ F     G  V G
Sbjct: 2   RFCVFGFL---SLFLIVSPASAWFFPNSTAVPPSLRNTTRVFWDAFSNFTGCHHGQNVDG 61

Query: 66  MSELKKYLNRFGYLPIPPENSFSDCFDDQFVSALILYQKRLGLSVTGKLDSETIASIMSP 125
           +  +KKY  RFGY+P     +F+D FDD   +A+ LYQ    L+VTG+LD+ TI  I+ P
Sbjct: 62  LYRIKKYFQRFGYIPETFSGNFTDDFDDILKAAVELYQTNFNLNVTGELDALTIQHIVIP 121

Query: 126 RCGMSDLIKIN-------------NTTTTTIHSTRRYAFFNGQPRWIR-ASTLTYALSPD 185
           RCG  D++                N + T +H+ +RY  F G+PRW R    LTYA  P 
Sbjct: 122 RCGNPDVVNGTSLMHGGRRKTFEVNFSRTHLHAVKRYTLFPGEPRWPRNRRDLTYAFDPK 181

Query: 186 YTIEYLTSSEIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEAFDGVL 245
             +    + E++ V  R+F RWS V  LNFT S  + +SDI IGFY GDHGDGE FDGVL
Sbjct: 182 NPL----TEEVKSVFSRAFGRWSDVTALNFTLSESFSTSDITIGFYTGDHGDGEPFDGVL 241

Query: 246 GVLAHAFSPENGRLHLDAAERWAVDFEKE---KSKVAVDLESVVTHEIGHVLGLAHSAVK 305
           G LAHAFSP +G+ HLDA E W V  + +       AVDLESV  HEIGH+LGL HS+V+
Sbjct: 242 GTLAHAFSPPSGKFHLDADENWVVSGDLDSFLSVTAAVDLESVAVHEIGHLLGLGHSSVE 301

Query: 306 EAVMYPSLSPRGKKVDLRIDDVEGVQTLYGSNPNF 321
           E++MYP+++   +KVDL  DDVEG+Q LYG+NPNF
Sbjct: 302 ESIMYPTITTGKRKVDLTNDDVEGIQYLYGANPNF 329

BLAST of Tan0007056 vs. TAIR 10
Match: AT1G59970.1 (Matrixin family protein )

HSP 1 Score: 252.7 bits (644), Expect = 4.5e-67
Identity = 142/341 (41.64%), Postives = 199/341 (58.36%), Query Frame = 0

Query: 9   LFFFYLLS--SVFLFSSVFPARTLPDFTTATDTDNNYAWRNFARFLDAGKGSEVTGMSEL 68
           L FF+ ++  S   +++V    ++P       T N  AW  F++      G  + G+S+L
Sbjct: 9   LIFFFTVNPISAKFYTNV---SSIPPLQFLNATQN--AWETFSKLAGCHIGENINGLSKL 68

Query: 69  KKYLNRFGYLPIPPENSFSDCFDDQFVSALILYQKRLGLSVTGKLDSETIASIMSPRCGM 128
           K+Y  RFGY  I    + +D FDD   SA+  YQK   L VTGKLDS T+  I+ PRCG 
Sbjct: 69  KQYFRRFGY--ITTTGNCTDDFDDVLQSAINTYQKNFNLKVTGKLDSSTLRQIVKPRCGN 128

Query: 129 SDLIK--INNTTTTTIHSTRRYAFFNGQPRW-IRASTLTYALSPDYTIEYLTSSEIRKVV 188
            DLI           + +T +Y+FF G+PRW  R   LTYA +P   +    + E+++V 
Sbjct: 129 PDLIDGVSEMNGGKILRTTEKYSFFPGKPRWPKRKRDLTYAFAPQNNL----TDEVKRVF 188

Query: 189 RRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEAFDGVLGVLAHAFSPENGRLH 248
            R+F+RW+ V PLNFT S     +DI IGF+ G+HGDGE FDG +G LAHA SP  G LH
Sbjct: 189 SRAFTRWAEVTPLNFTRSESILRADIVIGFFSGEHGDGEPFDGAMGTLAHASSPPTGMLH 248

Query: 249 LDAAERWAVDFEKEKSKV-----AVDLESVVTHEIGHVLGLAHSAVKEAVMYPSLSPRGK 308
           LD  E W +   +   ++      VDLESV  HEIGH+LGL HS+V++A+M+P++S   +
Sbjct: 249 LDGDEDWLISNGEISRRILPVTTVVDLESVAVHEIGHLLGLGHSSVEDAIMFPAISGGDR 308

Query: 309 KVDLRIDDVEGVQTLYGSNPNFKLKSFLESEKSINSTGSSI 340
           KV+L  DD+EG+Q LYG NPN        S +S ++ G S+
Sbjct: 309 KVELAKDDIEGIQHLYGGNPNGDGGGSKPSRESQSTGGDSV 338

BLAST of Tan0007056 vs. TAIR 10
Match: AT1G24140.1 (Matrixin family protein )

HSP 1 Score: 236.9 bits (603), Expect = 2.5e-62
Identity = 138/341 (40.47%), Postives = 186/341 (54.55%), Query Frame = 0

Query: 5   FRYYLFFFY---LLSSVFLFSSVFPARTLPDFTTATDTDNNYAWRNFARFLDAGKGSEVT 64
           F  +L FF    + +  +  SS  P + L + T          W +F  F     G +  
Sbjct: 7   FMVFLLFFAPSPVSAGFYTNSSAIPPQLLRNAT-------GNPWNSFLNFTGCHAGKKYD 66

Query: 65  GMSELKKYLNRFGYL-PIPPENSFSDCFDDQFVSALILYQKRLGLSVTGKLDSETIASIM 124
           G+  LK+Y   FGY+       +F+D FDD   +A+ +YQ+   L+VTG LD  T+  ++
Sbjct: 67  GLYMLKQYFQHFGYITETNLSGNFTDDFDDILKNAVEMYQRNFQLNVTGVLDELTLKHVV 126

Query: 125 SPRCGMSDLIKINNTTT----------------TTIHSTRRYAFFNGQPRWIR-ASTLTY 184
            PRCG  D+  +N T+T                   H+ + Y+FF G+PRW R    LTY
Sbjct: 127 IPRCGNPDV--VNGTSTMHSGRKTFEVSFAGRGQRFHAVKHYSFFPGEPRWPRNRRDLTY 186

Query: 185 ALSPDYTIEYLTSSEIRKVVRRSFSRWSAVIPLNFTESSDYESSDIRIGFYRGDHGDGEA 244
           A  P   +    + E++ V  R+F+RW  V PL FT    + +SDI IGFY G+HGDGE 
Sbjct: 187 AFDPRNAL----TEEVKSVFSRAFTRWEEVTPLTFTRVERFSTSDISIGFYSGEHGDGEP 246

Query: 245 FDGVLGVLAHAFSPENGRLHLDAAERWAVDFEKEKSKV----AVDLESVVTHEIGHVLGL 304
           FDG +  LAHAFSP  G  HLD  E W V  E     +    AVDLESV  HEIGH+LGL
Sbjct: 247 FDGPMRTLAHAFSPPTGHFHLDGEENWIVSGEGGDGFISVSEAVDLESVAVHEIGHLLGL 306

Query: 305 AHSAVKEAVMYPSLSPRGKKVDLRIDDVEGVQTLYGSNPNF 321
            HS+V+ ++MYP++    +KVDL  DDVEGVQ LYG+NPNF
Sbjct: 307 GHSSVEGSIMYPTIRTGRRKVDLTTDDVEGVQYLYGANPNF 334

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O235073.3e-9154.15Metalloendoproteinase 1-MMP OS=Arabidopsis thaliana OX=3702 GN=1MMP PE=1 SV=1[more]
Q8GWW61.2e-8550.89Metalloendoproteinase 4-MMP OS=Arabidopsis thaliana OX=3702 GN=4MMP PE=1 SV=1[more]
O045295.0e-7145.07Metalloendoproteinase 2-MMP OS=Arabidopsis thaliana OX=3702 GN=2MMP PE=1 SV=1[more]
Q9ZUJ56.3e-6641.64Metalloendoproteinase 5-MMP OS=Arabidopsis thaliana OX=3702 GN=5MMP PE=1 SV=1[more]
P291362.7e-6142.81Metalloendoproteinase 1 OS=Glycine max OX=3847 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
XP_023532756.11.6e-17589.94metalloendoproteinase 4-MMP-like [Cucurbita pepo subsp. pepo][more]
KAG7036120.11.0e-17489.47Metalloendoproteinase 1-MMP, partial [Cucurbita argyrosperma subsp. argyrosperma... [more]
XP_022957642.16.7e-17488.86metalloendoproteinase 4-MMP-like [Cucurbita moschata][more]
XP_022996404.13.7e-17289.36metalloendoproteinase 1-MMP-like [Cucurbita maxima][more]
XP_038875312.11.4e-17186.91metalloendoproteinase 1-MMP [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A6J1GZP33.3e-17488.86metalloendoproteinase 4-MMP-like OS=Cucurbita moschata OX=3662 GN=LOC111459118 P... [more]
A0A6J1K8M41.8e-17289.36metalloendoproteinase 1-MMP-like OS=Cucurbita maxima OX=3661 GN=LOC111491637 PE=... [more]
A0A6J1I2972.2e-17086.45metalloendoproteinase 1-MMP OS=Cucurbita maxima OX=3661 GN=LOC111468956 PE=3 SV=... [more]
A0A6J1E2L92.0e-16885.87metalloendoproteinase 1-MMP OS=Cucurbita moschata OX=3662 GN=LOC111430227 PE=3 S... [more]
A0A0A0KRA55.2e-16482.48ZnMc domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G162050 PE=3 S... [more]
Match NameE-valueIdentityDescription
AT4G16640.12.4e-9254.15Matrixin family protein [more]
AT2G45040.18.7e-8750.89Matrixin family protein [more]
AT1G70170.13.5e-7245.07matrix metalloproteinase [more]
AT1G59970.14.5e-6741.64Matrixin family protein [more]
AT1G24140.12.5e-6240.47Matrixin family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR021190Peptidase M10APRINTSPR00138MATRIXINcoord: 302..315
score: 57.67
coord: 207..235
score: 57.06
coord: 119..132
score: 36.85
coord: 183..198
score: 44.82
coord: 268..293
score: 53.56
IPR006026Peptidase, metallopeptidaseSMARTSM00235col_5coord: 151..316
e-value: 5.4E-41
score: 152.2
IPR002477Peptidoglycan binding-likePFAMPF01471PG_binding_1coord: 63..118
e-value: 2.6E-11
score: 43.6
IPR001818Peptidase M10, metallopeptidasePFAMPF00413Peptidase_M10coord: 154..315
e-value: 2.7E-49
score: 167.1
IPR024079Metallopeptidase, catalytic domain superfamilyGENE3D3.40.390.10Collagenase (Catalytic Domain)coord: 52..321
e-value: 1.5E-77
score: 262.4
NoneNo IPR availablePIRSRPIRSR001191-1PIRSR001191-1coord: 62..331
e-value: 1.0E-63
score: 213.4
NoneNo IPR availablePANTHERPTHR10201:SF245METALLOENDOPROTEINASE 4-MMPcoord: 31..352
NoneNo IPR availablePANTHERPTHR10201MATRIX METALLOPROTEINASEcoord: 31..352
NoneNo IPR availableSUPERFAMILY55486Metalloproteases ("zincins"), catalytic domaincoord: 152..318
IPR033739Peptidase M10A, catalytic domainCDDcd04278ZnMc_MMPcoord: 154..315
e-value: 3.23264E-65
score: 202.049
IPR036365PGBD-like superfamilySUPERFAMILY47090PGBD-likecoord: 54..129

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0007056.1Tan0007056.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0030574 collagen catabolic process
biological_process GO:0030198 extracellular matrix organization
biological_process GO:0006508 proteolysis
cellular_component GO:0031225 anchored component of membrane
cellular_component GO:0031012 extracellular matrix
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004222 metalloendopeptidase activity
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0008237 metallopeptidase activity