Tan0007031 (gene) Snake gourd v1

Overview
NameTan0007031
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionPatatin
LocationLG01: 7068677 .. 7071772 (-)
RNA-Seq ExpressionTan0007031
SyntenyTan0007031
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAAACTATGCCTGCAATTGAAATCAAAGCTCTCACTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCAATGGCACCTCTTTTCTCTTCTATTTCCCACCCCCTTTCTTCCCCAATTACAAACCCTAATCTCAATTTCACTTACAGATACGCTCTTCCTCTACGCACCCAAACTCTACGTTTCGTTTCCAATTCATCGTCTTCGCCATCGCCTCCACAGCCGCCACCGCCGGATCCCATTCCCGAGAAAAAGTCGCTCGCCGTAGCCACCGGGGAGCTCTTCTTGGGGCTGGCGGCGAGGCTGATTAAACGATCCTCTGACAAAAGCTCGAATGGGGTTGCTATGTTCGAGAATCGCAGTGGGACGAAGAGTGTGTACGGGGAGAGAATCGGCGCGGTGGTTGAGGATGAGATTCAGCCTGGTGTAGTGTGGGAGCAACGCGTTAAGGACGTTGAGGCCGAGCGAGAGCGGCGAGTCATTACCAGCCCTGGGTTTAGCTTCTCTGCCGCTGGTTTGCTGTTTCCGTACCATCTCGGAGTTGCGAAATTTCTCATTGAGAATGGTTATATCAAGGTTCTTCTGATTCTTTTCCCTTTCTTCTTGACGTGTTTTAGATTTTGGGTTGTTGTTCCGTTTGTGTCTATGTTGACGTGTTTCGTTGTGATTGAACTCTGAATGATGATGTGCTTGAATTGGTAAACAATTGGTCTTCTGGAAGAGTAGGCATTTGAGGAAATTTTCTCTCTTTTTAATGCATCTTTGTTTATGGGATATTTGCTGTGGAATATGAATGCTCTCCGCCTCTGACTCCCGTAGTGTATAAGACAATATTTAATAGCATAAAAATGTAGTTAAAAAGTTAGTAATTCGGGATGCTTAAGTTTAACAGTATGAGTCTTTTACAGTTGAAGTTTATTGATTACAATTTTTCTGGTTGGGAATGCAACTAGAGAGGTTTGTTTTTATAAGTACTTTGAAAGTATCTCCCTTCAGATGTCTTTGGATTGTAATGGAACCTTTTAGTTAAGGTTGAAGTTAGGAGAGAAAAGGTCACAAGAGGGTTTGCTACTGTTTTTTGATATGTATATTTATCTTGTTCTTGCGATGACTGATGAGTGATGACCCTCAAAAGTGTCTTTTTATCTTTGTTTTTTCCTCTTCATTGTTGTGTTGTCATTGTTATTATGAATTTTGTCATTTATGTTAATCCTTTTCTTATCTTCTTTGGTACTGAAGTGTTTGTAATGATATAGGAAACAACTCCATTAGCTGGAGCCTCAGCTGGTGCCATTGTCTGTGCCGTTATTGCTTCTGGAGCCAGTATGCAGGAAGCATTACAAGCTACTAAACTACTGGCCGAAGACTGCAGGAGTAGAGGGACTGCATTTCGCCTTGGGGTATTTCTCTGTTGAAGTAGGCCATTTCATTCATTGTGTTTATTTTTTGTGGTAAGACATTGAGTGGAAGGACGTATCACTTGAACTTCCTTTAGAAGAACTCCATTTTTTTTAATTCCTGTTATTTGTTGTGGTCTTAAGATTTATGATTTACATGTAGGTTTATTAGTATCCATGGCATTTCAAATTCTAGATATTGGTGTAGGATGGTTGAGGAGTGATATTGTAGGTCTCACTTGTCTTGATACTCAAGTTGCAAAGTGTTTGGACCAAGGTTGTTAACTTGTGAAACCAAACGACTATAGAAATTAAAATTGTATATCGCATATCGTAACATAACGTATATCGTAAGTTTCAATTTCATCCTCTTGCTTTAGGATCATTGTGACAGGGGAGAGGATCATTGTGACAGGGGAGATTTGCAGTTCTTTTTTTAACTCATCTACTGGTTCAAGTTTTAATGTTTTCCTCATTTAAATTCTCAGGCTGTACTCAGGGAAGTTCTTGAAAAGTTTCTTCCAGATGATGTACATATTAGGTGTAATGGAAGGGTTCGTGGTATGCATCCCTATTCATTCTCGTTGGTCTCCAAATAAAATCACCATATGAAGAGGGAAAGTGTTATATTTGTTGTGACATTGATCAACTCGATTATTGCAATCTGAAACTTTTGGTGGCTTTGATCTAATATATTTTTTTACAACATGTGTTGTAAAATGAGATGATACATGCATAAATTTTGGCTAAATAATTAATATTAAGATGAAGAAACTAGGTGTTTGGTTGGATCATCTGCCCCTGTGTTTTACTGTTCAAGCTCATTTTTAATTGGTTTGCATAATGTGATGGGAGAGCATTTCAACCACTTGCAATGCAATATCATTTTGGAAATTTTGATTTCTCATTTATATATATCTGTTATGTTCCAGTTGCTGTTACGCAAGTTTTTTGGAGGCCGAGAGGCTTGTTGGTGGATCAGTTTGATTCAAAAGATGATCTCATCAATGCAGTTTTCACTTCTTCCTTTATTCCAGGGTGAGTATTTTAATTTCACATCCTTTTGAATCTTCAAAATTTTCCTAGTTTGGTAAATGACCTTGGGCTGAAATAACGCTTTAGTGTAGATACCTAGCACCAAGACCCGTCACAGTCTTTCGGAATAGACTATGCATTGATGGTGGTCTTACATTGTTCATGCCACCAACTTCTGCCTCTCAGACGGTATTTACCTGATAAATTTCTGAATGATTTTCCTAATAATTTTAAGGTTAACCCAATTCATTAGAGACAAGATTTGAATATATTTCAATCACCCCGGAATTTTTCTTCGGGCTGTGTTGCAGGTGCGTGTATGTGCTTTCCCAGCTAGCAGATTGGGATTTGAAGGGATTGGGATCAGTCCAGACTGCAACCCTGAAAATAGAGCTGGCCCGAGAGAGGTAAATCATAAATGAGATGGACAAAGCTCGCCCAAGAAGTAGACACATTGTTATTTCCTCTTTGTAGTTTGATCAAACTCTTGCTTGTTCTGCATCATTTCAGCTTTTCAACTGGGCTCTTGAGCCAGCAGAAGATGACATTCTAGACCGGCTCTTTGACCTCGGATATCAGGATGCAGCTGTTTGGGCTGAGGATAATCCTGTTGAAAAGTTGGTGGAAGACGAAAGCACGATTGGGGACGACGCTTGTAACTAA

mRNA sequence

AAAAAAACTATGCCTGCAATTGAAATCAAAGCTCTCACTTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCTTCAATGGCACCTCTTTTCTCTTCTATTTCCCACCCCCTTTCTTCCCCAATTACAAACCCTAATCTCAATTTCACTTACAGATACGCTCTTCCTCTACGCACCCAAACTCTACGTTTCGTTTCCAATTCATCGTCTTCGCCATCGCCTCCACAGCCGCCACCGCCGGATCCCATTCCCGAGAAAAAGTCGCTCGCCGTAGCCACCGGGGAGCTCTTCTTGGGGCTGGCGGCGAGGCTGATTAAACGATCCTCTGACAAAAGCTCGAATGGGGTTGCTATGTTCGAGAATCGCAGTGGGACGAAGAGTGTGTACGGGGAGAGAATCGGCGCGGTGGTTGAGGATGAGATTCAGCCTGGTGTAGTGTGGGAGCAACGCGTTAAGGACGTTGAGGCCGAGCGAGAGCGGCGAGTCATTACCAGCCCTGGGTTTAGCTTCTCTGCCGCTGGTTTGCTGTTTCCGTACCATCTCGGAGTTGCGAAATTTCTCATTGAGAATGGTTATATCAAGGAAACAACTCCATTAGCTGGAGCCTCAGCTGGTGCCATTGTCTGTGCCGTTATTGCTTCTGGAGCCAGTATGCAGGAAGCATTACAAGCTACTAAACTACTGGCCGAAGACTGCAGGAGTAGAGGGACTGCATTTCGCCTTGGGGCTGTACTCAGGGAAGTTCTTGAAAAGTTTCTTCCAGATGATGTACATATTAGGTGTAATGGAAGGGTTCGTGTTGCTGTTACGCAAGTTTTTTGGAGGCCGAGAGGCTTGTTGGTGGATCAGTTTGATTCAAAAGATGATCTCATCAATGCAGTTTTCACTTCTTCCTTTATTCCAGGATACCTAGCACCAAGACCCGTCACAGTCTTTCGGAATAGACTATGCATTGATGGTGGTCTTACATTGTTCATGCCACCAACTTCTGCCTCTCAGACGGTGCGTGTATGTGCTTTCCCAGCTAGCAGATTGGGATTTGAAGGGATTGGGATCAGTCCAGACTGCAACCCTGAAAATAGAGCTGGCCCGAGAGAGCTTTTCAACTGGGCTCTTGAGCCAGCAGAAGATGACATTCTAGACCGGCTCTTTGACCTCGGATATCAGGATGCAGCTGTTTGGGCTGAGGATAATCCTGTTGAAAAGTTGGTGGAAGACGAAAGCACGATTGGGGACGACGCTTGTAACTAA

Coding sequence (CDS)

ATGGCACCTCTTTTCTCTTCTATTTCCCACCCCCTTTCTTCCCCAATTACAAACCCTAATCTCAATTTCACTTACAGATACGCTCTTCCTCTACGCACCCAAACTCTACGTTTCGTTTCCAATTCATCGTCTTCGCCATCGCCTCCACAGCCGCCACCGCCGGATCCCATTCCCGAGAAAAAGTCGCTCGCCGTAGCCACCGGGGAGCTCTTCTTGGGGCTGGCGGCGAGGCTGATTAAACGATCCTCTGACAAAAGCTCGAATGGGGTTGCTATGTTCGAGAATCGCAGTGGGACGAAGAGTGTGTACGGGGAGAGAATCGGCGCGGTGGTTGAGGATGAGATTCAGCCTGGTGTAGTGTGGGAGCAACGCGTTAAGGACGTTGAGGCCGAGCGAGAGCGGCGAGTCATTACCAGCCCTGGGTTTAGCTTCTCTGCCGCTGGTTTGCTGTTTCCGTACCATCTCGGAGTTGCGAAATTTCTCATTGAGAATGGTTATATCAAGGAAACAACTCCATTAGCTGGAGCCTCAGCTGGTGCCATTGTCTGTGCCGTTATTGCTTCTGGAGCCAGTATGCAGGAAGCATTACAAGCTACTAAACTACTGGCCGAAGACTGCAGGAGTAGAGGGACTGCATTTCGCCTTGGGGCTGTACTCAGGGAAGTTCTTGAAAAGTTTCTTCCAGATGATGTACATATTAGGTGTAATGGAAGGGTTCGTGTTGCTGTTACGCAAGTTTTTTGGAGGCCGAGAGGCTTGTTGGTGGATCAGTTTGATTCAAAAGATGATCTCATCAATGCAGTTTTCACTTCTTCCTTTATTCCAGGATACCTAGCACCAAGACCCGTCACAGTCTTTCGGAATAGACTATGCATTGATGGTGGTCTTACATTGTTCATGCCACCAACTTCTGCCTCTCAGACGGTGCGTGTATGTGCTTTCCCAGCTAGCAGATTGGGATTTGAAGGGATTGGGATCAGTCCAGACTGCAACCCTGAAAATAGAGCTGGCCCGAGAGAGCTTTTCAACTGGGCTCTTGAGCCAGCAGAAGATGACATTCTAGACCGGCTCTTTGACCTCGGATATCAGGATGCAGCTGTTTGGGCTGAGGATAATCCTGTTGAAAAGTTGGTGGAAGACGAAAGCACGATTGGGGACGACGCTTGTAACTAA

Protein sequence

MAPLFSSISHPLSSPITNPNLNFTYRYALPLRTQTLRFVSNSSSSPSPPQPPPPDPIPEKKSLAVATGELFLGLAARLIKRSSDKSSNGVAMFENRSGTKSVYGERIGAVVEDEIQPGVVWEQRVKDVEAERERRVITSPGFSFSAAGLLFPYHLGVAKFLIENGYIKETTPLAGASAGAIVCAVIASGASMQEALQATKLLAEDCRSRGTAFRLGAVLREVLEKFLPDDVHIRCNGRVRVAVTQVFWRPRGLLVDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTLFMPPTSASQTVRVCAFPASRLGFEGIGISPDCNPENRAGPRELFNWALEPAEDDILDRLFDLGYQDAAVWAEDNPVEKLVEDESTIGDDACN
Homology
BLAST of Tan0007031 vs. ExPASy Swiss-Prot
Match: Q8BJ56 (Patatin-like phospholipase domain-containing protein 2 OS=Mus musculus OX=10090 GN=Pnpla2 PE=1 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 1.2e-17
Identity = 77/250 (30.80%), Postives = 113/250 (45.20%), Query Frame = 0

Query: 138 TSPGFSFSAAGLLFPYHLGVAKFLIENG--YIKETTPLAGASAGAIVCAVIASGASMQEA 197
           T    SF+  G L  YH+GVA  L E+    +   T + GASAGA+    + +GA + EA
Sbjct: 6   TKWNISFAGCGFLGVYHIGVASCLREHAPFLVANATHIYGASAGALTATALVTGACLGEA 65

Query: 198 LQATKLLAEDCRSR-----GTAFRLGAVLREVLEKFLPDDVHIRCNGRVRVAVTQVFWRP 257
                 ++++ R R       +F L   +R  L K LP D H R NGR+ +++T+V    
Sbjct: 66  GANIIEVSKEARKRFLGPLHPSFNLVKTIRGCLLKTLPADCHERANGRLGISLTRVS-DG 125

Query: 258 RGLLVDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTLFMPPTSASQTVR 317
             +++  F SKD+LI A   S+FIP Y    P T+   R  +DGG++  +P      T+ 
Sbjct: 126 ENVIISHFSSKDELIQANVCSTFIPVYCGLIPPTLQGVRY-VDGGISDNLPLYELKNTIT 185

Query: 318 VCAFPASRLGFEGIGISPDCNPENRAGPREL--------FNW--------ALEPAEDDIL 365
           V  F          G S  C  ++     EL        FN         AL P E  +L
Sbjct: 186 VSPFS---------GESDICPQDSSTNIHELRVTNTSIQFNLRNLYRLSKALFPPEPMVL 244

BLAST of Tan0007031 vs. ExPASy Swiss-Prot
Match: Q9NST1 (1-acylglycerol-3-phosphate O-acyltransferase PNPLA3 OS=Homo sapiens OX=9606 GN=PNPLA3 PE=1 SV=2)

HSP 1 Score: 92.4 bits (228), Expect = 1.2e-17
Identity = 61/179 (34.08%), Postives = 95/179 (53.07%), Query Frame = 0

Query: 143 SFSAAGLLFPYHLGVAKFLIENG--YIKETTPLAGASAGAIVCAVIASGASMQEALQATK 202
           SF+  G L  YH+G  + L E+    +++   L GASAGA+ C  + SG  +++ LQ   
Sbjct: 11  SFAGCGFLGFYHVGATRCLSEHAPHLLRDARMLFGASAGALHCVGVLSGIPLEQTLQVLS 70

Query: 203 LLAEDCRSRG-----TAFRLGAVLREVLEKFLPDDVHIRCNGRVRVAVTQVFWRPRGLLV 262
            L    RSR       +F L   LR+ L K LP +VH   +G++ +++T+V      +LV
Sbjct: 71  DLVRKARSRNIGIFHPSFNLSKFLRQGLCKCLPANVHQLISGKIGISLTRVS-DGENVLV 130

Query: 263 DQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTLFMPPTSASQTVRVCAF 315
             F SKD++++A+  S FIP Y    P + FR    +DGG++  +P   A  T+ V  F
Sbjct: 131 SDFRSKDEVVDALVCSCFIPFYSGLIPPS-FRGVRYVDGGVSDNVPFIDAKTTITVSPF 187

BLAST of Tan0007031 vs. ExPASy Swiss-Prot
Match: P0C548 (Patatin-like phospholipase domain-containing protein 2 OS=Rattus norvegicus OX=10116 GN=Pnpla2 PE=1 SV=1)

HSP 1 Score: 91.3 bits (225), Expect = 2.6e-17
Identity = 76/250 (30.40%), Postives = 113/250 (45.20%), Query Frame = 0

Query: 138 TSPGFSFSAAGLLFPYHLGVAKFLIENG--YIKETTPLAGASAGAIVCAVIASGASMQEA 197
           T    SF+  G L  YH+GVA  L E+    +   T + GASAGA+    + +GA + EA
Sbjct: 6   TKWNISFAGCGFLGVYHIGVASCLREHAPFLVANATHIYGASAGALTATALVTGACLGEA 65

Query: 198 LQATKLLAEDCRSR-----GTAFRLGAVLREVLEKFLPDDVHIRCNGRVRVAVTQVFWRP 257
                 ++++ R R       +F L   +R  L K LP D H R +GR+ +++T+V    
Sbjct: 66  GANIIEVSKEARKRFLGPLHPSFNLVKTIRGCLLKTLPADCHTRASGRLGISLTRVS-DG 125

Query: 258 RGLLVDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTLFMPPTSASQTVR 317
             +++  F SKD+LI A   S+FIP Y    P T+   R  +DGG++  +P      T+ 
Sbjct: 126 ENVIISHFSSKDELIQANVCSTFIPVYCGLIPPTLQGVRY-VDGGISDNLPLYELKNTIT 185

Query: 318 VCAFPASRLGFEGIGISPDCNPENRAGPREL--------FNW--------ALEPAEDDIL 365
           V  F          G S  C  ++     EL        FN         AL P E  +L
Sbjct: 186 VSPFS---------GESDICPQDSSTNIHELRITNTSIQFNLRNLYRLSKALFPPEPMVL 244

BLAST of Tan0007031 vs. ExPASy Swiss-Prot
Match: P41247 (Patatin-like phospholipase domain-containing protein 4 OS=Homo sapiens OX=9606 GN=PNPLA4 PE=1 SV=3)

HSP 1 Score: 89.0 bits (219), Expect = 1.3e-16
Identity = 60/180 (33.33%), Postives = 90/180 (50.00%), Query Frame = 0

Query: 143 SFSAAGLLFPYHLGVAKFLIENG--YIKETTPLAGASAGAIVCAVIASG-ASMQEALQAT 202
           SF+A G L  YHLG A  L  +G   +K+    AGASAG++V +V+ +    ++E  Q T
Sbjct: 7   SFAACGFLGIYHLGAASALCRHGKKLVKDVKAFAGASAGSLVASVLLTAPEKIEECNQFT 66

Query: 203 KLLAEDCRSRG-----TAFRLGAVLREVLEKFLPDDVHIRCNGRVRVAVTQVFWRPRGLL 262
              AE+ R +        +   A LR  +E  LP   H     R+ V++T    R    L
Sbjct: 67  YKFAEEIRRQSFGAVTPGYDFMARLRSGMESILPPSAHELAQNRLHVSITNAKTR-ENHL 126

Query: 263 VDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTLFMPPTSASQTVRVCAF 315
           V  F S++DLI  +  SSF+P Y A   +  ++ +  +DGGLT  +P     +TV +  F
Sbjct: 127 VSTFSSREDLIKVLLASSFVPIY-AGLKLVEYKGQKWVDGGLTNALPILPVGRTVTISPF 184

BLAST of Tan0007031 vs. ExPASy Swiss-Prot
Match: Q91WW7 (1-acylglycerol-3-phosphate O-acyltransferase Pnpla3 OS=Mus musculus OX=10090 GN=Pnpla3 PE=1 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 6.5e-16
Identity = 68/247 (27.53%), Postives = 115/247 (46.56%), Query Frame = 0

Query: 143 SFSAAGLLFPYHLGVAKFLIENG--YIKETTPLAGASAGAIVCAVIASGASMQEALQATK 202
           SF+  G L  YH+G    L E     +++     G SAGA+          +   ++   
Sbjct: 11  SFAGCGFLGFYHVGATLCLSERAPHLLRDARTFFGCSAGALHAVTFVCSLPLGRIMEILM 70

Query: 203 LLAEDCRSR--GTA---FRLGAVLREVLEKFLPDDVHIRCNGRVRVAVTQVFWRPRGLLV 262
            L    RSR  GT    F +   +R+ L++ LPD+VH   +G+V +++T+V      +LV
Sbjct: 71  DLVRKARSRNIGTLHPFFNINKCIRDGLQESLPDNVHQVISGKVHISLTRVS-DGENVLV 130

Query: 263 DQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTLFMPPTSASQTVRVCAF- 322
            +F SKD++++A+  S FIP +    P + FR    +DGG++  +P   A  T+ V  F 
Sbjct: 131 SEFHSKDEVVDALVCSCFIPLFSGLIPPS-FRGERYVDGGVSDNVPVLDAKTTITVSPFY 190

Query: 323 -------PASRLGFEGIGISPDCNPENRAGPRELFNWALEPAEDDILDRLFDLGYQDAAV 375
                        F  + I+ + +     G  +L   AL P++  ++  L   GY DA  
Sbjct: 191 GEHDICPKVKSTNFFHVNIT-NLSLRLCTGNLQLLTRALFPSDVKVMGELCYQGYLDAFR 250

BLAST of Tan0007031 vs. NCBI nr
Match: XP_038878130.1 (patatin-like phospholipase domain-containing protein 4 [Benincasa hispida])

HSP 1 Score: 711.4 bits (1835), Expect = 4.1e-201
Identity = 356/383 (92.95%), Postives = 367/383 (95.82%), Query Frame = 0

Query: 3   PLFSSISHPLSSPITNPNLNFTYRYALPLRTQTLRFVSNSSSSPS----PPQPPPPDPIP 62
           P  SSISHP SSPITNPN NFTYRYALPLRTQTLRF+SNSSSSPS    PP PPPPDPIP
Sbjct: 4   PPLSSISHPFSSPITNPNFNFTYRYALPLRTQTLRFISNSSSSPSSPPPPPPPPPPDPIP 63

Query: 63  EKKSLAVATGELFLGLAARLIKRSSDKSSNGVAMFENRSGTKSVYGERIGAVVEDEIQPG 122
           EKKSLAVATGELFLGLAARLI RSSD+++N VAMF+NRSG KSV+ ERIGAVVEDEIQPG
Sbjct: 64  EKKSLAVATGELFLGLAARLINRSSDRTTNSVAMFDNRSGNKSVFEERIGAVVEDEIQPG 123

Query: 123 VVWEQRVKDVEAERERRVITSPGFSFSAAGLLFPYHLGVAKFLIENGYIKETTPLAGASA 182
           V+WEQRVKDVEAERERRVITSPGFSFSAAGLLFPYHLGVA FL+EN YIKETTPLAGASA
Sbjct: 124 VLWEQRVKDVEAERERRVITSPGFSFSAAGLLFPYHLGVANFLLENSYIKETTPLAGASA 183

Query: 183 GAIVCAVIASGASMQEALQATKLLAEDCRSRGTAFRLGAVLREVLEKFLPDDVHIRCNGR 242
           GAIVCAVIASGASMQEALQATKLLAEDCRSRGTAFRLGAVLREVL+KFLPDDVHIRCNGR
Sbjct: 184 GAIVCAVIASGASMQEALQATKLLAEDCRSRGTAFRLGAVLREVLDKFLPDDVHIRCNGR 243

Query: 243 VRVAVTQVFWRPRGLLVDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTL 302
           VRVAVTQVFWRPRGLLVDQFDSKDDLI AVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTL
Sbjct: 244 VRVAVTQVFWRPRGLLVDQFDSKDDLIEAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTL 303

Query: 303 FMPPTSASQTVRVCAFPASRLGFEGIGISPDCNPENRAGPRELFNWALEPAEDDILDRLF 362
           FMPPTSASQTVRVCAFPASRLGFEGIGISPDCNPENRAGPRELFNWALEPA+DDILD+LF
Sbjct: 304 FMPPTSASQTVRVCAFPASRLGFEGIGISPDCNPENRAGPRELFNWALEPADDDILDQLF 363

Query: 363 DLGYQDAAVWAEDNPVEKLVEDE 382
           DLGYQDAAVWAEDNPVEKLVEDE
Sbjct: 364 DLGYQDAAVWAEDNPVEKLVEDE 386

BLAST of Tan0007031 vs. NCBI nr
Match: KAG6600351.1 (Patatin-like phospholipase domain-containing protein 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 708.8 bits (1828), Expect = 2.7e-200
Identity = 355/381 (93.18%), Postives = 365/381 (95.80%), Query Frame = 0

Query: 1   MAPLFSSISHPLSSPITNPNLNFTYRYALPLRTQTLRFVSNSSSSPSPPQPPPPDPIPEK 60
           MAP  SSIS P SSPITNPNLNFT+RY LPLRTQTLRF SNSSSS SPPQPPPPDPIPEK
Sbjct: 88  MAPPLSSISRPFSSPITNPNLNFTHRYTLPLRTQTLRFTSNSSSSSSPPQPPPPDPIPEK 147

Query: 61  KSLAVATGELFLGLAARLIKRSSDKSSNGVAMFENRSGTKSVYGERIGAVVEDEIQPGVV 120
           KS AVATG+L LGLAARLI RSS KSSNGVAMFENRSGTKS Y ERIGAVVEDEIQP VV
Sbjct: 148 KSFAVATGDLLLGLAARLISRSSKKSSNGVAMFENRSGTKSEYEERIGAVVEDEIQPDVV 207

Query: 121 WEQRVKDVEAERERRVITSPGFSFSAAGLLFPYHLGVAKFLIENGYIKETTPLAGASAGA 180
           WEQR+KDV+AERERRV+TSPGFSFSAAGLLFPYHLGVAKFLIE+ YIKETTPLAGASAGA
Sbjct: 208 WEQRIKDVKAERERRVVTSPGFSFSAAGLLFPYHLGVAKFLIEHSYIKETTPLAGASAGA 267

Query: 181 IVCAVIASGASMQEALQATKLLAEDCRSRGTAFRLGAVLREVLEKFLPDDVHIRCNGRVR 240
           IVCAVIASGASM+EAL+ATKLLAEDCRSRGTAFRLGAVLREVLEKFLPDDVHIRCNGRVR
Sbjct: 268 IVCAVIASGASMKEALEATKLLAEDCRSRGTAFRLGAVLREVLEKFLPDDVHIRCNGRVR 327

Query: 241 VAVTQVFWRPRGLLVDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTLFM 300
           VAVTQVFWRPRGLLVDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTLFM
Sbjct: 328 VAVTQVFWRPRGLLVDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTLFM 387

Query: 301 PPTSASQTVRVCAFPASRLGFEGIGISPDCNPENRAGPRELFNWALEPAEDDILDRLFDL 360
           PPTSASQTVRVCAFPA+RLGFEGIGISPDCNPENRAGPRELFNWALEPAED ILDRLFDL
Sbjct: 388 PPTSASQTVRVCAFPATRLGFEGIGISPDCNPENRAGPRELFNWALEPAEDYILDRLFDL 447

Query: 361 GYQDAAVWAEDNPVEKLVEDE 382
           GYQDAAVWA+DNPVEKLVEDE
Sbjct: 448 GYQDAAVWAKDNPVEKLVEDE 468

BLAST of Tan0007031 vs. NCBI nr
Match: XP_023513040.1 (patatin-like phospholipase domain-containing protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 706.1 bits (1821), Expect = 1.7e-199
Identity = 353/381 (92.65%), Postives = 364/381 (95.54%), Query Frame = 0

Query: 1   MAPLFSSISHPLSSPITNPNLNFTYRYALPLRTQTLRFVSNSSSSPSPPQPPPPDPIPEK 60
           MAP  SSIS P SSPITNPN+NFT+RY LPLRTQTLRF SNSSSS SPPQPPPPDPIPEK
Sbjct: 1   MAPPLSSISRPFSSPITNPNINFTHRYTLPLRTQTLRFTSNSSSSSSPPQPPPPDPIPEK 60

Query: 61  KSLAVATGELFLGLAARLIKRSSDKSSNGVAMFENRSGTKSVYGERIGAVVEDEIQPGVV 120
           KS AVATG+L LGLAARLI RSS KSSNGVAMFENRSGTKS Y ERIGAVVEDEIQP VV
Sbjct: 61  KSFAVATGDLLLGLAARLISRSSKKSSNGVAMFENRSGTKSEYEERIGAVVEDEIQPDVV 120

Query: 121 WEQRVKDVEAERERRVITSPGFSFSAAGLLFPYHLGVAKFLIENGYIKETTPLAGASAGA 180
           WEQR+KDV+AERERRV+TSPGFSFSAAGLLFPYHLGVAKFLIE+ YIKETTPLAGASAGA
Sbjct: 121 WEQRIKDVKAERERRVVTSPGFSFSAAGLLFPYHLGVAKFLIEHSYIKETTPLAGASAGA 180

Query: 181 IVCAVIASGASMQEALQATKLLAEDCRSRGTAFRLGAVLREVLEKFLPDDVHIRCNGRVR 240
           IVCAVIASGASM+EAL+ATKLLAEDCRSRGTAFRLGAVLREVLEKFLPDDVHIRCNGRVR
Sbjct: 181 IVCAVIASGASMKEALEATKLLAEDCRSRGTAFRLGAVLREVLEKFLPDDVHIRCNGRVR 240

Query: 241 VAVTQVFWRPRGLLVDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTLFM 300
           VAVTQVFWRPRGLLVDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTLFM
Sbjct: 241 VAVTQVFWRPRGLLVDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTLFM 300

Query: 301 PPTSASQTVRVCAFPASRLGFEGIGISPDCNPENRAGPRELFNWALEPAEDDILDRLFDL 360
           PPTSASQTVRVCAFPA+RLGFEGIGISPDCNPENRAGPRELFNWALEPAED ILD LFDL
Sbjct: 301 PPTSASQTVRVCAFPATRLGFEGIGISPDCNPENRAGPRELFNWALEPAEDYILDHLFDL 360

Query: 361 GYQDAAVWAEDNPVEKLVEDE 382
           GYQDAAVWA+DNPVEKLVEDE
Sbjct: 361 GYQDAAVWAKDNPVEKLVEDE 381

BLAST of Tan0007031 vs. NCBI nr
Match: XP_022943045.1 (patatin-like phospholipase domain-containing protein 2 [Cucurbita moschata])

HSP 1 Score: 703.7 bits (1815), Expect = 8.6e-199
Identity = 355/383 (92.69%), Postives = 365/383 (95.30%), Query Frame = 0

Query: 1   MAPLFSSISHPLSSPITNPNLNFTYRYALPLRTQTLRFVSNSSSSPSPPQ--PPPPDPIP 60
           MAP  SSIS P SSPITNPNLNFT+RY LPLRTQTLRF SNSSSS SPPQ  PPPPDPIP
Sbjct: 1   MAPPLSSISRPFSSPITNPNLNFTHRYTLPLRTQTLRFTSNSSSSSSPPQPPPPPPDPIP 60

Query: 61  EKKSLAVATGELFLGLAARLIKRSSDKSSNGVAMFENRSGTKSVYGERIGAVVEDEIQPG 120
           EKKS AVATG+L LGLAARLI RSS KSSNGVAMFENRSGTKS Y ERIGAVVEDEIQP 
Sbjct: 61  EKKSFAVATGDLLLGLAARLISRSSKKSSNGVAMFENRSGTKSEYEERIGAVVEDEIQPD 120

Query: 121 VVWEQRVKDVEAERERRVITSPGFSFSAAGLLFPYHLGVAKFLIENGYIKETTPLAGASA 180
           VVWEQR+KDV+AERERRV+TSPGFSFSAAGLLFPYHLGVAKFLIE+ YIKETTPLAGASA
Sbjct: 121 VVWEQRIKDVKAERERRVVTSPGFSFSAAGLLFPYHLGVAKFLIEHSYIKETTPLAGASA 180

Query: 181 GAIVCAVIASGASMQEALQATKLLAEDCRSRGTAFRLGAVLREVLEKFLPDDVHIRCNGR 240
           GAIVCAVIASGASM+EAL+ATKLLAEDCRSRGTAFRLGAVLREVLEKFLPDDVHIRCNGR
Sbjct: 181 GAIVCAVIASGASMKEALEATKLLAEDCRSRGTAFRLGAVLREVLEKFLPDDVHIRCNGR 240

Query: 241 VRVAVTQVFWRPRGLLVDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTL 300
           VRVAVTQVFWRPRGLLVDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTL
Sbjct: 241 VRVAVTQVFWRPRGLLVDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTL 300

Query: 301 FMPPTSASQTVRVCAFPASRLGFEGIGISPDCNPENRAGPRELFNWALEPAEDDILDRLF 360
           FMPPTSASQTVRVCAFPA+RLGFEGIGISPDCNPENRAGPRELFNWALEPAED ILDRLF
Sbjct: 301 FMPPTSASQTVRVCAFPATRLGFEGIGISPDCNPENRAGPRELFNWALEPAEDYILDRLF 360

Query: 361 DLGYQDAAVWAEDNPVEKLVEDE 382
           DLGYQDAAVWA+DNPVEKLVEDE
Sbjct: 361 DLGYQDAAVWAKDNPVEKLVEDE 383

BLAST of Tan0007031 vs. NCBI nr
Match: XP_022981351.1 (patatin-like phospholipase domain-containing protein 4 [Cucurbita maxima])

HSP 1 Score: 698.0 bits (1800), Expect = 4.7e-197
Identity = 351/381 (92.13%), Postives = 361/381 (94.75%), Query Frame = 0

Query: 1   MAPLFSSISHPLSSPITNPNLNFTYRYALPLRTQTLRFVSNSSSSPSPPQPPPPDPIPEK 60
           MAP  SSIS   SSPITNPNLNFT+RY LPLRTQTLRF SNSSSS S PQPPPPDPIPEK
Sbjct: 1   MAPPLSSISRSFSSPITNPNLNFTHRYTLPLRTQTLRFTSNSSSSSSTPQPPPPDPIPEK 60

Query: 61  KSLAVATGELFLGLAARLIKRSSDKSSNGVAMFENRSGTKSVYGERIGAVVEDEIQPGVV 120
           KS AVATG+L LGLAARLI RSS KSSNGVAMFENRSGTKS Y ERIGAVVEDEIQP VV
Sbjct: 61  KSFAVATGDLLLGLAARLISRSSKKSSNGVAMFENRSGTKSEYEERIGAVVEDEIQPDVV 120

Query: 121 WEQRVKDVEAERERRVITSPGFSFSAAGLLFPYHLGVAKFLIENGYIKETTPLAGASAGA 180
           WEQR+KDV+AERERRV+TSPGFSFSAAGLLFPYHLGVAKFLIE+ YIKETTPLAGASAGA
Sbjct: 121 WEQRIKDVKAERERRVVTSPGFSFSAAGLLFPYHLGVAKFLIEHNYIKETTPLAGASAGA 180

Query: 181 IVCAVIASGASMQEALQATKLLAEDCRSRGTAFRLGAVLREVLEKFLPDDVHIRCNGRVR 240
           IVCAVIASGASM+EAL+ATKLLAEDCRSRGTAFRLGAVLREVLEKFLPDDVHIRCNGRVR
Sbjct: 181 IVCAVIASGASMKEALEATKLLAEDCRSRGTAFRLGAVLREVLEKFLPDDVHIRCNGRVR 240

Query: 241 VAVTQVFWRPRGLLVDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTLFM 300
           VAVTQVFWRPRGLLVDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTLFM
Sbjct: 241 VAVTQVFWRPRGLLVDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTLFM 300

Query: 301 PPTSASQTVRVCAFPASRLGFEGIGISPDCNPENRAGPRELFNWALEPAEDDILDRLFDL 360
           PPTSASQTV VCAFPA+RLGFEGIGISPDCNPENRAGPRELFNWALEPAED ILDRLFDL
Sbjct: 301 PPTSASQTVCVCAFPATRLGFEGIGISPDCNPENRAGPRELFNWALEPAEDYILDRLFDL 360

Query: 361 GYQDAAVWAEDNPVEKLVEDE 382
           GYQDA VWA+DNPVEKLVEDE
Sbjct: 361 GYQDAVVWAKDNPVEKLVEDE 381

BLAST of Tan0007031 vs. ExPASy TrEMBL
Match: A0A6J1FXB2 (Patatin OS=Cucurbita moschata OX=3662 GN=LOC111447900 PE=3 SV=1)

HSP 1 Score: 703.7 bits (1815), Expect = 4.2e-199
Identity = 355/383 (92.69%), Postives = 365/383 (95.30%), Query Frame = 0

Query: 1   MAPLFSSISHPLSSPITNPNLNFTYRYALPLRTQTLRFVSNSSSSPSPPQ--PPPPDPIP 60
           MAP  SSIS P SSPITNPNLNFT+RY LPLRTQTLRF SNSSSS SPPQ  PPPPDPIP
Sbjct: 1   MAPPLSSISRPFSSPITNPNLNFTHRYTLPLRTQTLRFTSNSSSSSSPPQPPPPPPDPIP 60

Query: 61  EKKSLAVATGELFLGLAARLIKRSSDKSSNGVAMFENRSGTKSVYGERIGAVVEDEIQPG 120
           EKKS AVATG+L LGLAARLI RSS KSSNGVAMFENRSGTKS Y ERIGAVVEDEIQP 
Sbjct: 61  EKKSFAVATGDLLLGLAARLISRSSKKSSNGVAMFENRSGTKSEYEERIGAVVEDEIQPD 120

Query: 121 VVWEQRVKDVEAERERRVITSPGFSFSAAGLLFPYHLGVAKFLIENGYIKETTPLAGASA 180
           VVWEQR+KDV+AERERRV+TSPGFSFSAAGLLFPYHLGVAKFLIE+ YIKETTPLAGASA
Sbjct: 121 VVWEQRIKDVKAERERRVVTSPGFSFSAAGLLFPYHLGVAKFLIEHSYIKETTPLAGASA 180

Query: 181 GAIVCAVIASGASMQEALQATKLLAEDCRSRGTAFRLGAVLREVLEKFLPDDVHIRCNGR 240
           GAIVCAVIASGASM+EAL+ATKLLAEDCRSRGTAFRLGAVLREVLEKFLPDDVHIRCNGR
Sbjct: 181 GAIVCAVIASGASMKEALEATKLLAEDCRSRGTAFRLGAVLREVLEKFLPDDVHIRCNGR 240

Query: 241 VRVAVTQVFWRPRGLLVDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTL 300
           VRVAVTQVFWRPRGLLVDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTL
Sbjct: 241 VRVAVTQVFWRPRGLLVDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTL 300

Query: 301 FMPPTSASQTVRVCAFPASRLGFEGIGISPDCNPENRAGPRELFNWALEPAEDDILDRLF 360
           FMPPTSASQTVRVCAFPA+RLGFEGIGISPDCNPENRAGPRELFNWALEPAED ILDRLF
Sbjct: 301 FMPPTSASQTVRVCAFPATRLGFEGIGISPDCNPENRAGPRELFNWALEPAEDYILDRLF 360

Query: 361 DLGYQDAAVWAEDNPVEKLVEDE 382
           DLGYQDAAVWA+DNPVEKLVEDE
Sbjct: 361 DLGYQDAAVWAKDNPVEKLVEDE 383

BLAST of Tan0007031 vs. ExPASy TrEMBL
Match: A0A6J1IWB3 (Patatin OS=Cucurbita maxima OX=3661 GN=LOC111480503 PE=3 SV=1)

HSP 1 Score: 698.0 bits (1800), Expect = 2.3e-197
Identity = 351/381 (92.13%), Postives = 361/381 (94.75%), Query Frame = 0

Query: 1   MAPLFSSISHPLSSPITNPNLNFTYRYALPLRTQTLRFVSNSSSSPSPPQPPPPDPIPEK 60
           MAP  SSIS   SSPITNPNLNFT+RY LPLRTQTLRF SNSSSS S PQPPPPDPIPEK
Sbjct: 1   MAPPLSSISRSFSSPITNPNLNFTHRYTLPLRTQTLRFTSNSSSSSSTPQPPPPDPIPEK 60

Query: 61  KSLAVATGELFLGLAARLIKRSSDKSSNGVAMFENRSGTKSVYGERIGAVVEDEIQPGVV 120
           KS AVATG+L LGLAARLI RSS KSSNGVAMFENRSGTKS Y ERIGAVVEDEIQP VV
Sbjct: 61  KSFAVATGDLLLGLAARLISRSSKKSSNGVAMFENRSGTKSEYEERIGAVVEDEIQPDVV 120

Query: 121 WEQRVKDVEAERERRVITSPGFSFSAAGLLFPYHLGVAKFLIENGYIKETTPLAGASAGA 180
           WEQR+KDV+AERERRV+TSPGFSFSAAGLLFPYHLGVAKFLIE+ YIKETTPLAGASAGA
Sbjct: 121 WEQRIKDVKAERERRVVTSPGFSFSAAGLLFPYHLGVAKFLIEHNYIKETTPLAGASAGA 180

Query: 181 IVCAVIASGASMQEALQATKLLAEDCRSRGTAFRLGAVLREVLEKFLPDDVHIRCNGRVR 240
           IVCAVIASGASM+EAL+ATKLLAEDCRSRGTAFRLGAVLREVLEKFLPDDVHIRCNGRVR
Sbjct: 181 IVCAVIASGASMKEALEATKLLAEDCRSRGTAFRLGAVLREVLEKFLPDDVHIRCNGRVR 240

Query: 241 VAVTQVFWRPRGLLVDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTLFM 300
           VAVTQVFWRPRGLLVDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTLFM
Sbjct: 241 VAVTQVFWRPRGLLVDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTLFM 300

Query: 301 PPTSASQTVRVCAFPASRLGFEGIGISPDCNPENRAGPRELFNWALEPAEDDILDRLFDL 360
           PPTSASQTV VCAFPA+RLGFEGIGISPDCNPENRAGPRELFNWALEPAED ILDRLFDL
Sbjct: 301 PPTSASQTVCVCAFPATRLGFEGIGISPDCNPENRAGPRELFNWALEPAEDYILDRLFDL 360

Query: 361 GYQDAAVWAEDNPVEKLVEDE 382
           GYQDA VWA+DNPVEKLVEDE
Sbjct: 361 GYQDAVVWAKDNPVEKLVEDE 381

BLAST of Tan0007031 vs. ExPASy TrEMBL
Match: A0A0A0L746 (Patatin OS=Cucumis sativus OX=3659 GN=Csa_3G214570 PE=3 SV=1)

HSP 1 Score: 693.0 bits (1787), Expect = 7.3e-196
Identity = 345/381 (90.55%), Postives = 366/381 (96.06%), Query Frame = 0

Query: 3   PLFSSISHPLSSPITNPNLNFTYRYALPLRTQTLRFVSNSSSS-PSPPQPPPPDPIPEKK 62
           P  SSISHP SSPITNPNLNFT+R++LPLR+QTLRF+SNSSSS P PP PPPPDPIPEK+
Sbjct: 4   PHLSSISHPFSSPITNPNLNFTHRFSLPLRSQTLRFISNSSSSPPPPPSPPPPDPIPEKR 63

Query: 63  SLAVATGELFLGLAARLIKRSSDKSSNGVAMFENRSG-TKSVYGERIGAVVEDEIQPGVV 122
           S AVATGELFLGLAARLIKRSSD++S  V+MF+NRSG  ++VY ERIGAVVEDEIQPGV+
Sbjct: 64  SFAVATGELFLGLAARLIKRSSDQTSKSVSMFDNRSGNNRNVYEERIGAVVEDEIQPGVL 123

Query: 123 WEQRVKDVEAERERRVITSPGFSFSAAGLLFPYHLGVAKFLIENGYIKETTPLAGASAGA 182
           WEQRVKDVEAERER ++TSPGFSFSAAGLLFPYHLGVAKFL+EN YIKETTPLAGASAGA
Sbjct: 124 WEQRVKDVEAERERPLVTSPGFSFSAAGLLFPYHLGVAKFLLENAYIKETTPLAGASAGA 183

Query: 183 IVCAVIASGASMQEALQATKLLAEDCRSRGTAFRLGAVLREVLEKFLPDDVHIRCNGRVR 242
           IVCAVIASGASMQEALQATK+LAEDCRSRGTAFRLGAVLREVL+KFLPDDVHIRCNGRVR
Sbjct: 184 IVCAVIASGASMQEALQATKILAEDCRSRGTAFRLGAVLREVLDKFLPDDVHIRCNGRVR 243

Query: 243 VAVTQVFWRPRGLLVDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTLFM 302
           VAVTQVFWRPRGLLVDQFDSKDDLI+AVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTLFM
Sbjct: 244 VAVTQVFWRPRGLLVDQFDSKDDLIDAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTLFM 303

Query: 303 PPTSASQTVRVCAFPASRLGFEGIGISPDCNPENRAGPRELFNWALEPAEDDILDRLFDL 362
           PPTSASQTVRVCAFPASRLGFEGIGISPDCNPENRAGPRELFNWALEPAEDDILD+LF+L
Sbjct: 304 PPTSASQTVRVCAFPASRLGFEGIGISPDCNPENRAGPRELFNWALEPAEDDILDQLFEL 363

Query: 363 GYQDAAVWAEDNPVEKLVEDE 382
           GYQDAAVW EDNPVEKLVEDE
Sbjct: 364 GYQDAAVWGEDNPVEKLVEDE 384

BLAST of Tan0007031 vs. ExPASy TrEMBL
Match: A0A5A7T6F4 (Patatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold252G00090 PE=3 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 1.1e-194
Identity = 347/384 (90.36%), Postives = 366/384 (95.31%), Query Frame = 0

Query: 3   PLFSSISHPL-SSPITNPNLNFTYRYALPLRTQTLRFVSNSSSS---PSPPQPPPPDPIP 62
           P  SSISHP  SSPITNPNLNFT R+ LPLR+QTLRF+SNSSSS   P PP PPPPDPIP
Sbjct: 4   PHLSSISHPFSSSPITNPNLNFTCRFPLPLRSQTLRFISNSSSSSSPPPPPSPPPPDPIP 63

Query: 63  EKKSLAVATGELFLGLAARLIKRSSDKSSNGVAMFENRSG-TKSVYGERIGAVVEDEIQP 122
           EK+SLAVATGELFLGLAARLIKRSSD++SN V+MF+NR+G  +SVY ERIGAVVEDEIQP
Sbjct: 64  EKRSLAVATGELFLGLAARLIKRSSDQTSNSVSMFDNRTGNNRSVYEERIGAVVEDEIQP 123

Query: 123 GVVWEQRVKDVEAERERRVITSPGFSFSAAGLLFPYHLGVAKFLIENGYIKETTPLAGAS 182
           GV+WEQRVKDVEAERER ++TSPGFSFSAAGLLFPYHLGVAKFL+EN YIKETTPLAGAS
Sbjct: 124 GVLWEQRVKDVEAERERPLVTSPGFSFSAAGLLFPYHLGVAKFLLENSYIKETTPLAGAS 183

Query: 183 AGAIVCAVIASGASMQEALQATKLLAEDCRSRGTAFRLGAVLREVLEKFLPDDVHIRCNG 242
           AGAIVCAVIASGASMQEALQATKLLAEDCRSRGTAFRLGAVLREVL+KFLPDDVHIRCNG
Sbjct: 184 AGAIVCAVIASGASMQEALQATKLLAEDCRSRGTAFRLGAVLREVLDKFLPDDVHIRCNG 243

Query: 243 RVRVAVTQVFWRPRGLLVDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLT 302
           RVRVAVTQVFWRPRGLLVDQFDSKDDLI+AVFTSSFIPGYLAPRPVTVFRNRLCIDGGLT
Sbjct: 244 RVRVAVTQVFWRPRGLLVDQFDSKDDLIDAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLT 303

Query: 303 LFMPPTSASQTVRVCAFPASRLGFEGIGISPDCNPENRAGPRELFNWALEPAEDDILDRL 362
           LFMPPTSASQTVRVCAFPASRLGFEGIGISPDCNPENRAGPRELFNWALEPAEDDILD+L
Sbjct: 304 LFMPPTSASQTVRVCAFPASRLGFEGIGISPDCNPENRAGPRELFNWALEPAEDDILDKL 363

Query: 363 FDLGYQDAAVWAEDNPVEKLVEDE 382
           F+LGY+DAAVW EDNPVEKLVEDE
Sbjct: 364 FELGYEDAAVWGEDNPVEKLVEDE 387

BLAST of Tan0007031 vs. ExPASy TrEMBL
Match: A0A1S3C2I5 (Patatin OS=Cucumis melo OX=3656 GN=LOC103496128 PE=3 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 1.1e-194
Identity = 347/384 (90.36%), Postives = 366/384 (95.31%), Query Frame = 0

Query: 3   PLFSSISHPL-SSPITNPNLNFTYRYALPLRTQTLRFVSNSSSS---PSPPQPPPPDPIP 62
           P  SSISHP  SSPITNPNLNFT R+ LPLR+QTLRF+SNSSSS   P PP PPPPDPIP
Sbjct: 4   PHLSSISHPFSSSPITNPNLNFTCRFPLPLRSQTLRFISNSSSSSSPPPPPSPPPPDPIP 63

Query: 63  EKKSLAVATGELFLGLAARLIKRSSDKSSNGVAMFENRSG-TKSVYGERIGAVVEDEIQP 122
           EK+SLAVATGELFLGLAARLIKRSSD++SN V+MF+NR+G  +SVY ERIGAVVEDEIQP
Sbjct: 64  EKRSLAVATGELFLGLAARLIKRSSDQTSNSVSMFDNRTGNNRSVYEERIGAVVEDEIQP 123

Query: 123 GVVWEQRVKDVEAERERRVITSPGFSFSAAGLLFPYHLGVAKFLIENGYIKETTPLAGAS 182
           GV+WEQRVKDVEAERER ++TSPGFSFSAAGLLFPYHLGVAKFL+EN YIKETTPLAGAS
Sbjct: 124 GVLWEQRVKDVEAERERPLVTSPGFSFSAAGLLFPYHLGVAKFLLENSYIKETTPLAGAS 183

Query: 183 AGAIVCAVIASGASMQEALQATKLLAEDCRSRGTAFRLGAVLREVLEKFLPDDVHIRCNG 242
           AGAIVCAVIASGASMQEALQATKLLAEDCRSRGTAFRLGAVLREVL+KFLPDDVHIRCNG
Sbjct: 184 AGAIVCAVIASGASMQEALQATKLLAEDCRSRGTAFRLGAVLREVLDKFLPDDVHIRCNG 243

Query: 243 RVRVAVTQVFWRPRGLLVDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLT 302
           RVRVAVTQVFWRPRGLLVDQFDSKDDLI+AVFTSSFIPGYLAPRPVTVFRNRLCIDGGLT
Sbjct: 244 RVRVAVTQVFWRPRGLLVDQFDSKDDLIDAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLT 303

Query: 303 LFMPPTSASQTVRVCAFPASRLGFEGIGISPDCNPENRAGPRELFNWALEPAEDDILDRL 362
           LFMPPTSASQTVRVCAFPASRLGFEGIGISPDCNPENRAGPRELFNWALEPAEDDILD+L
Sbjct: 304 LFMPPTSASQTVRVCAFPASRLGFEGIGISPDCNPENRAGPRELFNWALEPAEDDILDKL 363

Query: 363 FDLGYQDAAVWAEDNPVEKLVEDE 382
           F+LGY+DAAVW EDNPVEKLVEDE
Sbjct: 364 FELGYEDAAVWGEDNPVEKLVEDE 387

BLAST of Tan0007031 vs. TAIR 10
Match: AT1G33270.1 (Acyl transferase/acyl hydrolase/lysophospholipase superfamily protein )

HSP 1 Score: 480.3 bits (1235), Expect = 1.4e-135
Identity = 235/323 (72.76%), Postives = 273/323 (84.52%), Query Frame = 0

Query: 60  KKSLAVATGELFLGLAARLIKRSSDKSSNGVAMFENRSGTKSVYGERIGAVVEDEIQPGV 119
           K+S AVATGELF+G+A+RL+K S+ K+                 G+RI +V+EDEI+P +
Sbjct: 50  KRSFAVATGELFIGIASRLLKSSNQKT------------PPIDDGDRIASVIEDEIEPAM 109

Query: 120 VWEQRVKDVEAERERRVITSPGFSFSAAGLLFPYHLGVAKFLIENGYIKETTPLAGASAG 179
           +WEQRVKDVEAE+ERRVITSPGFSFSAAGLLFPYHLGVA+ LIE GYIKETTPLAG+SAG
Sbjct: 110 IWEQRVKDVEAEKERRVITSPGFSFSAAGLLFPYHLGVAQLLIEKGYIKETTPLAGSSAG 169

Query: 180 AIVCAVIASGASMQEALQATKLLAEDCRSRGTAFRLGAVLREVLEKFLPDDVHIRCNGRV 239
           AIVCAVI SGA+M+EAL+ATK LA DCR  GTAFRLGAVLRE +E+ LPDD+HIR NGR+
Sbjct: 170 AIVCAVITSGATMREALEATKELAYDCRRNGTAFRLGAVLRESMERLLPDDIHIRSNGRI 229

Query: 240 RVAVTQVFWRPRGLLVDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTLF 299
           RVA+TQVFWRPRGLLVDQFDSK DLI+AVFTSSFIPGYLAPRP T+FRNRLC+DGGLTLF
Sbjct: 230 RVAITQVFWRPRGLLVDQFDSKSDLIDAVFTSSFIPGYLAPRPATMFRNRLCVDGGLTLF 289

Query: 300 MPPTSASQTVRVCAFPASRLGFEGIGISPDCNPENRAGPRELFNWALEPAEDDILDRLFD 359
           MPPT+A++TVRVCAF AS    +GI I PDCNP NRA  R+L NWALEPAED++L+RLF+
Sbjct: 290 MPPTAAAKTVRVCAFSASNFKLKGIEICPDCNPLNRATSRQLLNWALEPAEDEVLERLFE 349

Query: 360 LGYQDAAVWAEDNPVEKLVEDES 383
           LGY DAA WAE NPVE LV D++
Sbjct: 350 LGYADAATWAEMNPVEGLVYDDT 360

BLAST of Tan0007031 vs. TAIR 10
Match: AT1G33270.2 (Acyl transferase/acyl hydrolase/lysophospholipase superfamily protein )

HSP 1 Score: 377.5 bits (968), Expect = 1.3e-104
Identity = 186/250 (74.40%), Postives = 216/250 (86.40%), Query Frame = 0

Query: 60  KKSLAVATGELFLGLAARLIKRSSDKSSNGVAMFENRSGTKSVYGERIGAVVEDEIQPGV 119
           K+S AVATGELF+G+A+RL+K S+ K+                 G+RI +V+EDEI+P +
Sbjct: 50  KRSFAVATGELFIGIASRLLKSSNQKT------------PPIDDGDRIASVIEDEIEPAM 109

Query: 120 VWEQRVKDVEAERERRVITSPGFSFSAAGLLFPYHLGVAKFLIENGYIKETTPLAGASAG 179
           +WEQRVKDVEAE+ERRVITSPGFSFSAAGLLFPYHLGVA+ LIE GYIKETTPLAG+SAG
Sbjct: 110 IWEQRVKDVEAEKERRVITSPGFSFSAAGLLFPYHLGVAQLLIEKGYIKETTPLAGSSAG 169

Query: 180 AIVCAVIASGASMQEALQATKLLAEDCRSRGTAFRLGAVLREVLEKFLPDDVHIRCNGRV 239
           AIVCAVI SGA+M+EAL+ATK LA DCR  GTAFRLGAVLRE +E+ LPDD+HIR NGR+
Sbjct: 170 AIVCAVITSGATMREALEATKELAYDCRRNGTAFRLGAVLRESMERLLPDDIHIRSNGRI 229

Query: 240 RVAVTQVFWRPRGLLVDQFDSKDDLINAVFTSSFIPGYLAPRPVTVFRNRLCIDGGLTLF 299
           RVA+TQVFWRPRGLLVDQFDSK DLI+AVFTSSFIPGYLAPRP T+FRNRLC+DGGLTLF
Sbjct: 230 RVAITQVFWRPRGLLVDQFDSKSDLIDAVFTSSFIPGYLAPRPATMFRNRLCVDGGLTLF 287

Query: 300 MPPTSASQTV 310
           MPPT+A++TV
Sbjct: 290 MPPTAAAKTV 287

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8BJ561.2e-1730.80Patatin-like phospholipase domain-containing protein 2 OS=Mus musculus OX=10090 ... [more]
Q9NST11.2e-1734.081-acylglycerol-3-phosphate O-acyltransferase PNPLA3 OS=Homo sapiens OX=9606 GN=P... [more]
P0C5482.6e-1730.40Patatin-like phospholipase domain-containing protein 2 OS=Rattus norvegicus OX=1... [more]
P412471.3e-1633.33Patatin-like phospholipase domain-containing protein 4 OS=Homo sapiens OX=9606 G... [more]
Q91WW76.5e-1627.531-acylglycerol-3-phosphate O-acyltransferase Pnpla3 OS=Mus musculus OX=10090 GN=... [more]
Match NameE-valueIdentityDescription
XP_038878130.14.1e-20192.95patatin-like phospholipase domain-containing protein 4 [Benincasa hispida][more]
KAG6600351.12.7e-20093.18Patatin-like phospholipase domain-containing protein 2, partial [Cucurbita argyr... [more]
XP_023513040.11.7e-19992.65patatin-like phospholipase domain-containing protein 2 [Cucurbita pepo subsp. pe... [more]
XP_022943045.18.6e-19992.69patatin-like phospholipase domain-containing protein 2 [Cucurbita moschata][more]
XP_022981351.14.7e-19792.13patatin-like phospholipase domain-containing protein 4 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1FXB24.2e-19992.69Patatin OS=Cucurbita moschata OX=3662 GN=LOC111447900 PE=3 SV=1[more]
A0A6J1IWB32.3e-19792.13Patatin OS=Cucurbita maxima OX=3661 GN=LOC111480503 PE=3 SV=1[more]
A0A0A0L7467.3e-19690.55Patatin OS=Cucumis sativus OX=3659 GN=Csa_3G214570 PE=3 SV=1[more]
A0A5A7T6F41.1e-19490.36Patatin OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold252G00090 PE=3 S... [more]
A0A1S3C2I51.1e-19490.36Patatin OS=Cucumis melo OX=3656 GN=LOC103496128 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G33270.11.4e-13572.76Acyl transferase/acyl hydrolase/lysophospholipase superfamily protein [more]
AT1G33270.21.3e-10474.40Acyl transferase/acyl hydrolase/lysophospholipase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002641Patatin-like phospholipase domainPFAMPF01734Patatincoord: 144..297
e-value: 3.0E-8
score: 34.1
IPR002641Patatin-like phospholipase domainPROSITEPS51635PNPLAcoord: 142..307
score: 21.118673
NoneNo IPR availableGENE3D3.40.1090.10Cytosolic phospholipase A2 catalytic domaincoord: 122..232
e-value: 2.2E-8
score: 36.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 39..60
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 45..59
NoneNo IPR availablePANTHERPTHR12406:SF43BNAC07G30920D PROTEINcoord: 29..382
NoneNo IPR availableCDDcd07224Pat_likecoord: 141..370
e-value: 1.575E-132
score: 377.449
IPR033562Patatin-like phospholipase domain-containing proteinPANTHERPTHR12406CALCIUM-INDEPENDENT PHOSPHOLIPASE A2 IPLA2 -RELATEDcoord: 29..382
IPR016035Acyl transferase/acyl hydrolase/lysophospholipaseSUPERFAMILY52151FabD/lysophospholipase-likecoord: 141..363

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0007031.1Tan0007031.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055088 lipid homeostasis
biological_process GO:0019433 triglyceride catabolic process
biological_process GO:0016042 lipid catabolic process
biological_process GO:0006629 lipid metabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005811 lipid droplet
cellular_component GO:0016020 membrane
molecular_function GO:0004806 triglyceride lipase activity
molecular_function GO:0016787 hydrolase activity