Tan0006864 (gene) Snake gourd v1

BLAST of Tan0006864 vs. ExPASy Swiss-Prot
Match: F4JUU5 (Membrane-bound transcription factor site-2 protease homolog OS=Arabidopsis thaliana OX=3702 GN=S2P PE=2 SV=2)

HSP 1 Score: 415.6 bits (1067), Expect = 8.5e-115
Identity = 233/511 (45.60%), Postives = 316/511 (61.84%), Query Frame = 0

Query: 29  TSRRGLSNAISCWYCDCKITSFNEPIFHFGRRHARVLRAWFSTGIGFSLAALAAVTTVLF 88
           T    + N  SC YCD KI++FNEPIF  GRR + VL+ WFS G+GF +A+L  VT  L 
Sbjct: 21  TGGENIENEASCCYCDLKISNFNEPIFRLGRRFSGVLKVWFSIGLGFGVASLILVTVFLL 80

Query: 89  MELAIAMHIFGNNNVLRGLPISSSSLFGLPPLISSSSFAPADAGYIIISTLISVAFHEFG 148
           ++   +  +F N          +S++FG  P   S+  + +   Y+++ST+I+V+ HE G
Sbjct: 81  LQFH-SNPLFSNR--------LTSAVFGFSP---STRVSLSGIAYVLVSTVITVSVHELG 140

Query: 149 HAAAAASEGIKLEYVAVFIALLFPGALVAFNHDALQDSSCFNALRIYCAGIWHNAVLSAA 208
           HA AAASEGI++EY+AVFIA +FPG LVAF++D LQ    FNALRIYCAGIWHNAV  A 
Sbjct: 141 HALAAASEGIQMEYIAVFIAAIFPGGLVAFDNDVLQSLPSFNALRIYCAGIWHNAVFCAL 200

Query: 209 SGLILFFLPLILFPLYIHGESPMVLNVPFTSPLSGYLSHGHVILSLDGIHVRSVDDWINL 268
               LF LP++L P Y HGES  V++VP  SPL GYLS G VI+SLDGI V    +W+ L
Sbjct: 201 CVFALFLLPVMLSPFYKHGESLTVVDVPSVSPLFGYLSPGDVIVSLDGIQVHKPSEWLEL 260

Query: 269 STQISELTFQNETLS-RLGENNRMANGRKEYCVPNFMLKESNKVQFTHDRLACFGDLTSF 328
           +  + +   +    S  LG + R  +G K YCVP  +++E  K +   ++  C GDLT+F
Sbjct: 261 AAILDKENSKTSNGSLYLGGSRRFHHG-KGYCVPISLIEEGYKGKMVENQFVCPGDLTAF 320

Query: 329 TSIPCVSSTALIDGDVEDSHSNRKERIYCLNPSDVIKLDKCTRQWDKSISNDSNSTCMCS 388
            ++PC             S++  +E   CL+  D++KL KC   W  +   D+ S C+C 
Sbjct: 321 RTMPC-------------SNAAIREVSVCLDAKDIVKLQKCGDGWVTTSDTDNQSDCVCP 380

Query: 389 QDETCLSPVHVPGLVWVEITYLNPHSSDCFYSREYL-LPSSNCSGTFIFVGDVVSMARSI 448
           Q + CL  +  PG++W EITY    S DC  SR  L   +SNC GTF+FVGD+++M+ S+
Sbjct: 381 QGDLCLQAMQSPGVLWTEITYKRTSSQDC--SRLGLDFNTSNCLGTFVFVGDLIAMSHSV 440

Query: 449 QLTMYRPRLDFH-FATYLPDVLERILLCLFHASLALALLNSFPVYYLDGESILEIIIFQL 508
            LT Y+PR  F+ F    P++LER L C FH SLAL LLNS PVYYLDGESILE  +   
Sbjct: 441 HLTAYQPRWLFNFFGKSFPNILERSLTCTFHVSLALVLLNSLPVYYLDGESILESSLQSF 500

Query: 509 TSMSPRNKEKVLRSFLMGGTLMSTFLLLRIF 537
           T +SPR K+K L+  L+GG+L+S     RIF
Sbjct: 501 TWLSPRKKKKALQVCLVGGSLLSFLAFFRIF 503

BLAST of Tan0006864 vs. ExPASy Swiss-Prot
Match: O54862 (Membrane-bound transcription factor site-2 protease OS=Cricetulus griseus OX=10029 GN=MBTPS2 PE=2 SV=1)

HSP 1 Score: 118.6 bits (296), Expect = 2.2e-25
Identity = 134/512 (26.17%), Postives = 214/512 (41.80%), Query Frame = 0

Query: 31  RRGLSNAISCWYCDCKITSFNEPIFHFGRRHARVLRAWFSTGIGFSLAALAA----VTTV 90
           + GLS  IS ++   + + FN   + +GRR AR+L  WF+ G+ F + A+ +    +   
Sbjct: 40  KNGLS--ISPFHIRWQTSVFNRAFYSWGRRKARMLYQWFNFGMVFGVIAMFSSFFLLGKT 99

Query: 91  LFMELAIAMHIFGNNNVLRGLPISSSSLFG------LPPLISSSSFAPADAGYIIISTLI 150
           L   LA  M    +++       SSSS         L  ++   +       Y   + LI
Sbjct: 100 LMQTLAQMMADSPSSSSSSSSSSSSSSSSSIHNEQVLQVVVPGINLPVNQLTYFFAAVLI 159

Query: 151 SVAFHEFGHAAAAASEGIKLEYVAVFIALLFPGALVAFNHDALQDSSCFNALRIYCAGIW 210
           S   HE GH  AA  E ++     +F+ +++PGA V      LQ  S    LRI+CAGIW
Sbjct: 160 SGVVHEIGHGIAAIREQVRFNGFGIFLFIIYPGAFVDLFTTHLQLISPVQQLRIFCAGIW 219

Query: 211 HNAVLSAASGLILFFLPLILFPLYIHGESPMVLNVPFTSPLSG--YLSHGHVILSLDGIH 270
           HN VL+    L L  LP+IL P Y  G   ++  V   SP  G   L  G ++  L    
Sbjct: 220 HNFVLALLGILALVLLPVILLPFYYTGVGVLITEVAEDSPAIGPRGLFVGDLVTHLQDCP 279

Query: 271 VRSVDDWINLSTQISELTFQNETLSRLGENNRMANGRKEYCVPNFMLKESNKVQFTHDRL 330
           V +V DW             NE L  +    ++      YC+    L++ +     + RL
Sbjct: 280 VTNVQDW-------------NECLDTIAYEPQIG-----YCISASTLQQLSFPVRAYKRL 339

Query: 331 ACFGDLTSFTSIPCVSSTALIDGDVEDSHSNRKERIYCLNPSDVIKLDKCTRQWDKSISN 390
                     S  C ++ +L D      ++  K    CL     ++  +  R      ++
Sbjct: 340 D--------GSTECCNNHSLTDVCFSYRNNFNKRLHTCLPARKAVEATQVCRTNKDCKTS 399

Query: 391 DSNSTCMCSQDETCLSPVHVPGLVWVEITYLNPHSSDCFYSREYLLPSSNCSGTFIFVGD 450
            S+S C+    ET    + V           +P   D  Y                 VG 
Sbjct: 400 SSSSFCIVPSLETHTRLIKVK----------HPPQIDMLY-----------------VGH 459

Query: 451 VVSMARSIQLTMYRPRLDFHFATYLPDVLERILLCLFHASLALALLNSFPVYYLDGESIL 510
            + +  ++ +T + PR +F  +  LP ++E  +  L   S ALA++N+ P + LDG+ IL
Sbjct: 460 PLHLHYTVSITSFIPRFNF-LSIDLPVIVETFVKYLISLSGALAIVNAVPCFALDGQWIL 495

Query: 511 EIII-FQLTSMSPRNKEKVLRSF--LMGGTLM 528
              +   LTS+   N  K L  F  L+GG+++
Sbjct: 520 NSFLDATLTSVIGDNDVKDLIGFFILLGGSVL 495

BLAST of Tan0006864 vs. ExPASy Swiss-Prot
Match: Q8CHX6 (Membrane-bound transcription factor site-2 protease OS=Mus musculus OX=10090 GN=Mbtps2 PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 6.3e-25
Identity = 135/514 (26.26%), Postives = 208/514 (40.47%), Query Frame = 0

Query: 37  AISCWYCDCKITSFNEPIFHFGRRHARVLRAWFSTGIGFSLAALAAVTTVLFMELAIAMH 96
           +IS ++   + + FN   + +GRR AR+L  WF+ G+ F + A+ +   +L   L   M 
Sbjct: 44  SISPFHIRWQTSIFNRAFYSWGRRKARMLYQWFNFGMVFGVIAMFSSFFLLGKTL---MQ 103

Query: 97  IFGNNNVLRGLPISSSSLFGLPPLISSSSFAPA------------------DAGYIIIST 156
                      P SSSS        SSSS +                       Y   + 
Sbjct: 104 TLAQMMADSPSPYSSSSSSSSSSSSSSSSSSSLHNEQVLQVVVPGINLPVNQLTYFFAAV 163

Query: 157 LISVAFHEFGHAAAAASEGIKLEYVAVFIALLFPGALVAFNHDALQDSSCFNALRIYCAG 216
           LIS   HE GH  AA  E ++     +F+ +++PGA V      LQ  S    LRI+CAG
Sbjct: 164 LISGVVHEIGHGIAAIREQVRFNGFGIFLFIIYPGAFVDLFTTHLQLISPVQQLRIFCAG 223

Query: 217 IWHNAVLSAASGLILFFLPLILFPLYIHGESPMVLNVPFTSPLSG--YLSHGHVILSLDG 276
           IWHN VL+    L L  LP+IL P Y  G   ++  V   SP  G   L  G ++  L  
Sbjct: 224 IWHNFVLALLGILALVLLPVILLPFYYTGVGVLITEVAEDSPAIGPRGLFVGDLVTHLQD 283

Query: 277 IHVRSVDDWINLSTQISELTFQNETLSRLGENNRMANGRKEYCVPNFMLKESNKVQFTHD 336
             V +V DW             NE L  +    ++      YC+    L++ +     + 
Sbjct: 284 CPVTNVQDW-------------NECLDTIAYEPQIG-----YCISASTLQQLSFPVRAYK 343

Query: 337 RLACFGDLTSFTSIPCVSSTALIDGDVEDSHSNRKERIYCLNPSDVIKLDKCTRQWDKSI 396
           RL          S  C ++ +L D      ++  K    CL     ++  +  R      
Sbjct: 344 RLD--------GSTECCNNHSLTDVCFSYRNNFNKRLHTCLPARKAVEATQVCRSNKDCK 403

Query: 397 SNDSNSTCMCSQDETCLSPVHVPGLVWVEITYLNPHSSDCFYSREYLLPSSNCSGTFIFV 456
           S  S+S C+    ET    + V           +P   D  Y                 V
Sbjct: 404 SGASSSFCIVPSLETHTRLIKVK----------HPPQIDMLY-----------------V 463

Query: 457 GDVVSMARSIQLTMYRPRLDFHFATYLPDVLERILLCLFHASLALALLNSFPVYYLDGES 516
           G  + +  ++ +T + PR +F  +  LP ++E  +  L   S ALA++N+ P + LDG+ 
Sbjct: 464 GHPLHLHYTVSITSFIPRFNF-LSIDLPVIVETFVKYLISLSGALAIVNAVPCFALDGQW 500

Query: 517 ILEIII-FQLTSMSPRNKEKVLRSF--LMGGTLM 528
           IL   +   LTS+   N  K L  F  L+GG+++
Sbjct: 524 ILNSFLDATLTSVIGDNDVKDLIGFFILLGGSVL 500

BLAST of Tan0006864 vs. ExPASy Swiss-Prot
Match: Q5RAC8 (Membrane-bound transcription factor site-2 protease OS=Pongo abelii OX=9601 GN=MBTPS2 PE=2 SV=1)

HSP 1 Score: 115.2 bits (287), Expect = 2.4e-24
Identity = 134/517 (25.92%), Postives = 211/517 (40.81%), Query Frame = 0

Query: 37  AISCWYCDCKITSFNEPIFHFGRRHARVLRAWFSTGIGFSLAALAAVTTVLFMEL--AIA 96
           +IS ++   +   FN   + +GRR AR+L  WF+ G+ F + A+ +   +L   L   +A
Sbjct: 44  SISPFHIRWQTAVFNRAFYSWGRRKARMLYQWFNFGMVFGVIAMFSSFFLLGKTLMQTLA 103

Query: 97  MHIFGNNNVLRGLPISSSSLFGLPPLISSSSFAPA-------------------DAGYII 156
             +  + +       SSSS        SSSS + +                      Y  
Sbjct: 104 QMMADSPSSYSSSSSSSSSSSSSSSSSSSSSSSSSLHNEQVLQVVVPGINLPVNQLTYFF 163

Query: 157 ISTLISVAFHEFGHAAAAASEGIKLEYVAVFIALLFPGALVAFNHDALQDSSCFNALRIY 216
            + LIS   HE GH  AA  E ++     +F+ +++PGA V      LQ  S    LRI+
Sbjct: 164 AAVLISGVVHEIGHGIAAIREQVRFNGFGIFLFIIYPGAFVDLFTTHLQLISPVQQLRIF 223

Query: 217 CAGIWHNAVLSAASGLILFFLPLILFPLYIHGESPMVLNVPFTSPLSG--YLSHGHVILS 276
           CAGIWHN VL+    L L  LP+IL P Y  G   ++  V   SP  G   L  G ++  
Sbjct: 224 CAGIWHNFVLALLGILALVLLPVILLPFYYTGVGVLITEVAEDSPAIGPRGLFVGDLVTH 283

Query: 277 LDGIHVRSVDDWINLSTQISELTFQNETLSRLGENNRMANGRKEYCVPNFMLKESNKVQF 336
           L    V +V DW             NE L  +    ++      YC+    L++ +    
Sbjct: 284 LQDCPVTNVQDW-------------NECLDTIAYEPQIG-----YCISASTLQQLSFPVR 343

Query: 337 THDRLACFGDLTSFTSIPCVSSTALIDGDVEDSHSNRKERIYCLNPSDVIKLDKCTRQWD 396
            + RL          S  C ++ +L D      ++  K    CL     ++  +  R   
Sbjct: 344 AYKRLD--------GSTECCNNHSLTDVCFSYRNNFNKRLHTCLPARKAVEATQVCRTNK 403

Query: 397 KSISNDSNSTCMCSQDETCLSPVHVPGLVWVEITYLNPHSSDCFYSREYLLPSSNCSGTF 456
               + S+S C+    ET    + V           +P   D  Y               
Sbjct: 404 DCKKSSSSSFCIIPSLETHTRLIKVK----------HPPQIDMLY--------------- 463

Query: 457 IFVGDVVSMARSIQLTMYRPRLDFHFATYLPDVLERILLCLFHASLALALLNSFPVYYLD 516
             VG  + +  ++ +T + PR +F  +  LP V+E  +  L   S ALA++N+ P + LD
Sbjct: 464 --VGHPLHLHYTVSITSFIPRFNF-LSIDLPVVVETFVKYLISLSGALAIVNAVPCFALD 506

Query: 517 GESILEIII-FQLTSMSPRNKEKVLRSF--LMGGTLM 528
           G+ IL   +   LTS+   N  K L  F  L+GG+++
Sbjct: 524 GQWILNSFLDATLTSVIGDNDVKDLIGFFILLGGSVL 506

BLAST of Tan0006864 vs. ExPASy Swiss-Prot
Match: O43462 (Membrane-bound transcription factor site-2 protease OS=Homo sapiens OX=9606 GN=MBTPS2 PE=1 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 3.1e-24
Identity = 134/515 (26.02%), Postives = 208/515 (40.39%), Query Frame = 0

Query: 37  AISCWYCDCKITSFNEPIFHFGRRHARVLRAWFSTGIGFSLAALAAVTTVL---FMELAI 96
           +IS ++   +   FN   + +GRR AR+L  WF+ G+ F + A+ +   +L    M+   
Sbjct: 44  SISPFHIRWQTAVFNRAFYSWGRRKARMLYQWFNFGMVFGVIAMFSSFFLLGKTLMQTLA 103

Query: 97  AMHIFGNNNVLRGLPISSSSLFGLPPLISSSS----------------FAPADAGYIIIS 156
            M     ++       SSSS        SSSS                       Y   +
Sbjct: 104 QMMADSPSSYSSSSSSSSSSSSSSSSSSSSSSSLHNEQVLQVVVPGINLPVNQLTYFFTA 163

Query: 157 TLISVAFHEFGHAAAAASEGIKLEYVAVFIALLFPGALVAFNHDALQDSSCFNALRIYCA 216
            LIS   HE GH  AA  E ++     +F+ +++PGA V      LQ  S    LRI+CA
Sbjct: 164 VLISGVVHEIGHGIAAIREQVRFNGFGIFLFIIYPGAFVDLFTTHLQLISPVQQLRIFCA 223

Query: 217 GIWHNAVLSAASGLILFFLPLILFPLYIHGESPMVLNVPFTSPLSG--YLSHGHVILSLD 276
           GIWHN VL+    L L  LP+IL P Y  G   ++  V   SP  G   L  G ++  L 
Sbjct: 224 GIWHNFVLALLGILALVLLPVILLPFYYTGVGVLITEVAEDSPAIGPRGLFVGDLVTHLQ 283

Query: 277 GIHVRSVDDWINLSTQISELTFQNETLSRLGENNRMANGRKEYCVPNFMLKESNKVQFTH 336
              V +V DW             NE L  +    ++      YC+    L++ +     +
Sbjct: 284 DCPVTNVQDW-------------NECLDTIAYEPQIG-----YCISASTLQQLSFPVRAY 343

Query: 337 DRLACFGDLTSFTSIPCVSSTALIDGDVEDSHSNRKERIYCLNPSDVIKLDKCTRQWDKS 396
            RL          S  C ++ +L D      ++  K    CL     ++  +  R     
Sbjct: 344 KRLD--------GSTECCNNHSLTDVCFSYRNNFNKRLHTCLPARKAVEATQVCRTNKDC 403

Query: 397 ISNDSNSTCMCSQDETCLSPVHVPGLVWVEITYLNPHSSDCFYSREYLLPSSNCSGTFIF 456
             + S+S C+    ET    + V           +P   D  Y                 
Sbjct: 404 KKSSSSSFCIIPSLETHTRLIKVK----------HPPQIDMLY----------------- 463

Query: 457 VGDVVSMARSIQLTMYRPRLDFHFATYLPDVLERILLCLFHASLALALLNSFPVYYLDGE 516
           VG  + +  ++ +T + PR +F  +  LP V+E  +  L   S ALA++N+ P + LDG+
Sbjct: 464 VGHPLHLHYTVSITSFIPRFNF-LSIDLPVVVETFVKYLISLSGALAIVNAVPCFALDGQ 504

Query: 517 SILEIII-FQLTSMSPRNKEKVLRSF--LMGGTLM 528
            IL   +   LTS+   N  K L  F  L+GG+++
Sbjct: 524 WILNSFLDATLTSVIGDNDVKDLIGFFILLGGSVL 504

BLAST of Tan0006864 vs. NCBI nr
Match: XP_022957694.1 (membrane-bound transcription factor site-2 protease homolog [Cucurbita moschata])

HSP 1 Score: 953.0 bits (2462), Expect = 1.1e-273
Identity = 473/542 (87.27%), Postives = 505/542 (93.17%), Query Frame = 0

Query: 1   MSGRWRRSKRPMAQADAHARIPPLLPLSTSRRGLSNAISCWYCDCKITSFNEPIFHFGRR 60
           MSGRWR SKRP A+ADAHAR+PPLLPLSTSR+GLSNA+SCWYCDCKITSFNEPIFHFGRR
Sbjct: 1   MSGRWRLSKRPRAEADAHARVPPLLPLSTSRKGLSNAVSCWYCDCKITSFNEPIFHFGRR 60

Query: 61  HARVLRAWFSTGIGFSLAALAAVTTVLFMELAIAMHIFGNNNVLRGLPISSSSLFGLPPL 120
           HARVLRAWFS GIGFSLAALA VTTVLF+ELAIAMHIFGN++V   LPIS SSLFGLPPL
Sbjct: 61  HARVLRAWFSIGIGFSLAALAVVTTVLFLELAIAMHIFGNSDVPHSLPISYSSLFGLPPL 120

Query: 121 ISSSSFAPADAGYIIISTLISVAFHEFGHAAAAASEGIKLEYVAVFIALLFPGALVAFNH 180
           ISS +F+PADAGYIIIS+LISVAFHEFGHAAA ASEGIKLEYVAVFIALLFPGALVAFNH
Sbjct: 121 ISSCNFSPADAGYIIISSLISVAFHEFGHAAAVASEGIKLEYVAVFIALLFPGALVAFNH 180

Query: 181 DALQDSSCFNALRIYCAGIWHNAVLSAASGLILFFLPLILFPLYIHGESPMVLNVPFTSP 240
           D LQDSSCFNALRIYCAGIWHNAVLSAASGL+LF LPLILFPLYIHGESPMVL+VP TSP
Sbjct: 181 DVLQDSSCFNALRIYCAGIWHNAVLSAASGLMLFCLPLILFPLYIHGESPMVLDVPSTSP 240

Query: 241 LSGYLSHGHVILSLDGIHVRSVDDWINLSTQISELTFQNETLSRLGENNRMANGRKEYCV 300
           LSGYLSHGH+ILSLDG+H+++VDDW+NLS QISE TFQN TLSRLGEN+RMANGRK YCV
Sbjct: 241 LSGYLSHGHLILSLDGMHIQNVDDWVNLSAQISESTFQNGTLSRLGENDRMANGRKGYCV 300

Query: 301 PNFMLKESNKVQFTHDRLACFGDLTSFTSIPCVSSTALIDGDVEDSHSNRKERIYCLNPS 360
           PNFMLKESNKVQF+HD+  CFGDLTSFTSIPCVSST L+DGDV+DSH NRKE I+CLN +
Sbjct: 301 PNFMLKESNKVQFSHDQSTCFGDLTSFTSIPCVSSTVLVDGDVDDSHYNRKEGIFCLNVN 360

Query: 361 DVIKLDKCTRQWDKSISNDSNSTCMCSQDETCLSPVHVPGLVWVEITYLNPHSSDCFYSR 420
           D+IKL+KC   WDK+I NDS STCMCSQDETCLSPV +PG VWVEITYLNPHSSDCFYSR
Sbjct: 361 DIIKLNKCISGWDKAIINDSTSTCMCSQDETCLSPVQMPGSVWVEITYLNPHSSDCFYSR 420

Query: 421 EYLLPSSNCSGTFIFVGDVVSMARSIQLTMYRPRLDFHFATYLPDVLERILLCLFHASLA 480
           E  LPSSNCSGTFIFVGDVVSMA SIQLTMYRPRLDFH+A YLPDVLERI LCLFHASLA
Sbjct: 421 ENPLPSSNCSGTFIFVGDVVSMAHSIQLTMYRPRLDFHYARYLPDVLERIFLCLFHASLA 480

Query: 481 LALLNSFPVYYLDGESILEIIIFQLTSMSPRNKEKVLRSFLMGGTLMSTFLLLRIFFHLF 540
           LALLNS PVYYLDGESILEIIIFQLTSMSPRNKEKVLR+FLMGGTLMS FLLLRIFFH+F
Sbjct: 481 LALLNSLPVYYLDGESILEIIIFQLTSMSPRNKEKVLRAFLMGGTLMSIFLLLRIFFHVF 540

Query: 541 VS 543
           VS
Sbjct: 541 VS 542

BLAST of Tan0006864 vs. NCBI nr
Match: KAG6605818.1 (Membrane-bound transcription factor site-2 protease-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 949.1 bits (2452), Expect = 1.6e-272
Identity = 470/542 (86.72%), Postives = 504/542 (92.99%), Query Frame = 0

Query: 1   MSGRWRRSKRPMAQADAHARIPPLLPLSTSRRGLSNAISCWYCDCKITSFNEPIFHFGRR 60
           MSGRWR SKRP A+ADAHAR+PPLLPLSTSR+GLSNA+SCWYCDCKITSFNEPIFHFGRR
Sbjct: 1   MSGRWRLSKRPRAEADAHARVPPLLPLSTSRKGLSNAVSCWYCDCKITSFNEPIFHFGRR 60

Query: 61  HARVLRAWFSTGIGFSLAALAAVTTVLFMELAIAMHIFGNNNVLRGLPISSSSLFGLPPL 120
           H RVLRAWFS GIGFSLAALA VTTVLF+ELA+AMHIFGN++V   LPIS SSLFGLPPL
Sbjct: 61  HGRVLRAWFSIGIGFSLAALAVVTTVLFLELAVAMHIFGNSDVPHSLPISYSSLFGLPPL 120

Query: 121 ISSSSFAPADAGYIIISTLISVAFHEFGHAAAAASEGIKLEYVAVFIALLFPGALVAFNH 180
           ISS +F+PADAGYIIIS+LISVAFHEFGHAAA ASEGIKLEYVAVFIALLFPGALVAFNH
Sbjct: 121 ISSCNFSPADAGYIIISSLISVAFHEFGHAAAVASEGIKLEYVAVFIALLFPGALVAFNH 180

Query: 181 DALQDSSCFNALRIYCAGIWHNAVLSAASGLILFFLPLILFPLYIHGESPMVLNVPFTSP 240
           D LQDSSCFNALRIYCAGIWHNAVLSAASGL+LF LPLILFPLYIHGESPMVL+VP TSP
Sbjct: 181 DVLQDSSCFNALRIYCAGIWHNAVLSAASGLMLFCLPLILFPLYIHGESPMVLDVPSTSP 240

Query: 241 LSGYLSHGHVILSLDGIHVRSVDDWINLSTQISELTFQNETLSRLGENNRMANGRKEYCV 300
           LSGYLSHGH+ILSLDG+H+++VDDW+NLS QISE TFQN TLSRLGEN+RMANGRK YCV
Sbjct: 241 LSGYLSHGHLILSLDGMHIQNVDDWVNLSAQISESTFQNGTLSRLGENDRMANGRKGYCV 300

Query: 301 PNFMLKESNKVQFTHDRLACFGDLTSFTSIPCVSSTALIDGDVEDSHSNRKERIYCLNPS 360
           PNFMLKESNKVQF+HD+  CFGDLTSFTSIPCVSST L+DGDV+DSH NRKE I+CLN +
Sbjct: 301 PNFMLKESNKVQFSHDQSTCFGDLTSFTSIPCVSSTVLVDGDVDDSHYNRKEGIFCLNVN 360

Query: 361 DVIKLDKCTRQWDKSISNDSNSTCMCSQDETCLSPVHVPGLVWVEITYLNPHSSDCFYSR 420
           D+IKL+KC   W+K+I NDS STCMCSQDETCLSPV +PG VWVEITYLNPHSSDCFYSR
Sbjct: 361 DIIKLNKCISGWNKAIINDSTSTCMCSQDETCLSPVQMPGSVWVEITYLNPHSSDCFYSR 420

Query: 421 EYLLPSSNCSGTFIFVGDVVSMARSIQLTMYRPRLDFHFATYLPDVLERILLCLFHASLA 480
           E  LPSSNCSGTFIFVGDVVSMA SIQLTMYRPRLDFH+A YLPDVLERI LCLFHASLA
Sbjct: 421 ENPLPSSNCSGTFIFVGDVVSMAHSIQLTMYRPRLDFHYARYLPDVLERIFLCLFHASLA 480

Query: 481 LALLNSFPVYYLDGESILEIIIFQLTSMSPRNKEKVLRSFLMGGTLMSTFLLLRIFFHLF 540
           LALLNS PVYYLDGESILEIIIFQLTSMSPRNKEKVLR+FLMGGTLMS FLLLRIFFH+F
Sbjct: 481 LALLNSLPVYYLDGESILEIIIFQLTSMSPRNKEKVLRAFLMGGTLMSIFLLLRIFFHVF 540

Query: 541 VS 543
           VS
Sbjct: 541 VS 542

BLAST of Tan0006864 vs. NCBI nr
Match: XP_023534713.1 (membrane-bound transcription factor site-2 protease homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 946.8 bits (2446), Expect = 8.1e-272
Identity = 472/542 (87.08%), Postives = 503/542 (92.80%), Query Frame = 0

Query: 1   MSGRWRRSKRPMAQADAHARIPPLLPLSTSRRGLSNAISCWYCDCKITSFNEPIFHFGRR 60
           MSGRWR SKRP A+ADAHAR+PPLLPLSTSR+GLSNA+SCWYCDCKITSFNEPIFHFGRR
Sbjct: 1   MSGRWRLSKRPRAEADAHARVPPLLPLSTSRKGLSNAVSCWYCDCKITSFNEPIFHFGRR 60

Query: 61  HARVLRAWFSTGIGFSLAALAAVTTVLFMELAIAMHIFGNNNVLRGLPISSSSLFGLPPL 120
           HARVLRAWFS GIGFSLAALA VTTVLF+ELAIAMHIFGN++V   LPIS SSLFGLPPL
Sbjct: 61  HARVLRAWFSIGIGFSLAALAVVTTVLFLELAIAMHIFGNSDVPHSLPISYSSLFGLPPL 120

Query: 121 ISSSSFAPADAGYIIISTLISVAFHEFGHAAAAASEGIKLEYVAVFIALLFPGALVAFNH 180
           ISS +FAPADAGYIIIS+LISVAFHEFGHAAA ASEGIKLEYVAVFIALLFPGALVAFNH
Sbjct: 121 ISSCNFAPADAGYIIISSLISVAFHEFGHAAAVASEGIKLEYVAVFIALLFPGALVAFNH 180

Query: 181 DALQDSSCFNALRIYCAGIWHNAVLSAASGLILFFLPLILFPLYIHGESPMVLNVPFTSP 240
           D LQDSSCFNALRIYCAGIWHNAVLSAASGL+LF LPLILFPLY+ GESPMVL+VP TSP
Sbjct: 181 DVLQDSSCFNALRIYCAGIWHNAVLSAASGLMLFCLPLILFPLYLRGESPMVLDVPSTSP 240

Query: 241 LSGYLSHGHVILSLDGIHVRSVDDWINLSTQISELTFQNETLSRLGENNRMANGRKEYCV 300
           LSGYLSHGH+ILSLDG+H+++VDDW+NLS QISE TFQN TLSRLGEN+RMANGRK YCV
Sbjct: 241 LSGYLSHGHLILSLDGMHIQNVDDWVNLSAQISESTFQNGTLSRLGENDRMANGRKGYCV 300

Query: 301 PNFMLKESNKVQFTHDRLACFGDLTSFTSIPCVSSTALIDGDVEDSHSNRKERIYCLNPS 360
           PNFMLKESNKVQF+HD+  CFGDLTSFTSIPCVSST L+DGDV+DSHSNRKE I+CLN +
Sbjct: 301 PNFMLKESNKVQFSHDQSTCFGDLTSFTSIPCVSSTVLVDGDVDDSHSNRKEGIFCLNVN 360

Query: 361 DVIKLDKCTRQWDKSISNDSNSTCMCSQDETCLSPVHVPGLVWVEITYLNPHSSDCFYSR 420
           DVIKL+KC   WDK+I NDS STCMCSQDETCLSPV +PG VWVEITYLNPHSSDCFYSR
Sbjct: 361 DVIKLNKCISGWDKAIINDSTSTCMCSQDETCLSPVQMPGSVWVEITYLNPHSSDCFYSR 420

Query: 421 EYLLPSSNCSGTFIFVGDVVSMARSIQLTMYRPRLDFHFATYLPDVLERILLCLFHASLA 480
           E  LPS NCSGTFIFVGDVVSMA SIQLTMYRPRLDFH+A YLPDVLERI LCLFHASLA
Sbjct: 421 ENPLPSLNCSGTFIFVGDVVSMAHSIQLTMYRPRLDFHYARYLPDVLERIFLCLFHASLA 480

Query: 481 LALLNSFPVYYLDGESILEIIIFQLTSMSPRNKEKVLRSFLMGGTLMSTFLLLRIFFHLF 540
           LALLNS PVYYLDGESILEIIIFQLTSMS RNKEKVLR+FLMGGTLMS FLLLRIFFH+F
Sbjct: 481 LALLNSLPVYYLDGESILEIIIFQLTSMSLRNKEKVLRAFLMGGTLMSIFLLLRIFFHVF 540

Query: 541 VS 543
           VS
Sbjct: 541 VS 542

BLAST of Tan0006864 vs. NCBI nr
Match: XP_022995357.1 (membrane-bound transcription factor site-2 protease homolog [Cucurbita maxima])

HSP 1 Score: 944.9 bits (2441), Expect = 3.1e-271
Identity = 469/542 (86.53%), Postives = 503/542 (92.80%), Query Frame = 0

Query: 1   MSGRWRRSKRPMAQADAHARIPPLLPLSTSRRGLSNAISCWYCDCKITSFNEPIFHFGRR 60
           M GRWR SKRP A+ADA+AR+PPLLPLSTSR+GLSNA+SCWYCDCKITSFNEPIFHFGRR
Sbjct: 1   MPGRWRLSKRPRAEADANARVPPLLPLSTSRKGLSNAVSCWYCDCKITSFNEPIFHFGRR 60

Query: 61  HARVLRAWFSTGIGFSLAALAAVTTVLFMELAIAMHIFGNNNVLRGLPISSSSLFGLPPL 120
           HARVLRAWFS GIGFSLAALA VTTVLF+ELAI MHIFGN++V   LPIS SSLFGLPPL
Sbjct: 61  HARVLRAWFSIGIGFSLAALAVVTTVLFLELAITMHIFGNSDVPHSLPISYSSLFGLPPL 120

Query: 121 ISSSSFAPADAGYIIISTLISVAFHEFGHAAAAASEGIKLEYVAVFIALLFPGALVAFNH 180
           ISS +FAPADAGYIIIS+LISVAFHEFGHAAA ASEGIKLEYVAVFIALLFPGALVAFNH
Sbjct: 121 ISSCNFAPADAGYIIISSLISVAFHEFGHAAAVASEGIKLEYVAVFIALLFPGALVAFNH 180

Query: 181 DALQDSSCFNALRIYCAGIWHNAVLSAASGLILFFLPLILFPLYIHGESPMVLNVPFTSP 240
           D LQDSSCFNALRIYCAGIWHNAVLSAASGL+LF LPLILFPLYIHGESPMVL+VP TSP
Sbjct: 181 DVLQDSSCFNALRIYCAGIWHNAVLSAASGLMLFCLPLILFPLYIHGESPMVLDVPSTSP 240

Query: 241 LSGYLSHGHVILSLDGIHVRSVDDWINLSTQISELTFQNETLSRLGENNRMANGRKEYCV 300
           LSGYLSHGH+ILSLDG+H+++VDDW+NLS QISE TFQN TLSRLGEN++MANGRK YCV
Sbjct: 241 LSGYLSHGHLILSLDGMHIQNVDDWVNLSVQISESTFQNGTLSRLGENDQMANGRKGYCV 300

Query: 301 PNFMLKESNKVQFTHDRLACFGDLTSFTSIPCVSSTALIDGDVEDSHSNRKERIYCLNPS 360
           PNFMLKESNKVQF+HD+  CFGDLTSFTSIPCVSST L+DGDV+DSH NRKE I+CLN +
Sbjct: 301 PNFMLKESNKVQFSHDQSTCFGDLTSFTSIPCVSSTVLVDGDVDDSHYNRKEGIFCLNVN 360

Query: 361 DVIKLDKCTRQWDKSISNDSNSTCMCSQDETCLSPVHVPGLVWVEITYLNPHSSDCFYSR 420
           DVIKL+KC   WDK+I NDS STCMCSQDETCLSPV +PG +WVEITYLNPHSSDCFYSR
Sbjct: 361 DVIKLNKCISGWDKAIINDSTSTCMCSQDETCLSPVQMPGSIWVEITYLNPHSSDCFYSR 420

Query: 421 EYLLPSSNCSGTFIFVGDVVSMARSIQLTMYRPRLDFHFATYLPDVLERILLCLFHASLA 480
           E  LPSSNCSGTFIFVGDVVSMA SIQLTMY+PRLDFH+A YLPDVLERI LCLFHASLA
Sbjct: 421 ENPLPSSNCSGTFIFVGDVVSMAHSIQLTMYQPRLDFHYARYLPDVLERIFLCLFHASLA 480

Query: 481 LALLNSFPVYYLDGESILEIIIFQLTSMSPRNKEKVLRSFLMGGTLMSTFLLLRIFFHLF 540
           LALLNS PVYYLDGESILEIIIFQLTSMSPRNKEKVLR+FLMGGTLMS FLLLRIFFH+F
Sbjct: 481 LALLNSLPVYYLDGESILEIIIFQLTSMSPRNKEKVLRAFLMGGTLMSIFLLLRIFFHVF 540

Query: 541 VS 543
           VS
Sbjct: 541 VS 542

BLAST of Tan0006864 vs. NCBI nr
Match: XP_004145951.1 (membrane-bound transcription factor site-2 protease homolog isoform X1 [Cucumis sativus] >KGN49826.1 hypothetical protein Csa_000136 [Cucumis sativus])

HSP 1 Score: 906.4 bits (2341), Expect = 1.2e-259
Identity = 457/542 (84.32%), Postives = 488/542 (90.04%), Query Frame = 0

Query: 1   MSGRWRRSKRPMAQADAHARIPPLLPLSTSRRGLSNAISCWYCDCKITSFNEPIFHFGRR 60
           M GRWRRSKR MAQADAHA IPPLLPL+TSR+GLSN+ISCWYCD KITSFNE IF FGRR
Sbjct: 1   MPGRWRRSKRLMAQADAHAHIPPLLPLTTSRKGLSNSISCWYCDYKITSFNELIFQFGRR 60

Query: 61  HARVLRAWFSTGIGFSLAALAAVTTVLFMELAIAMHIFGNNNVLRGLPISSSSLFGLPPL 120
           HARVLR WFS GIGFSLAALA V TVLF EL I MHIFG +NV+RGLP+S SSLFGLP L
Sbjct: 61  HARVLRTWFSIGIGFSLAALAVVATVLFRELTIVMHIFGKSNVIRGLPVSCSSLFGLPSL 120

Query: 121 ISSSSFAPADAGYIIISTLISVAFHEFGHAAAAASEGIKLEYVAVFIALLFPGALVAFNH 180
           ISS S  PA AGYIIISTLISVAFHEFGHAAAAASEG+KLEY+AVFIALLFPGALVAFN+
Sbjct: 121 ISSCSLFPAGAGYIIISTLISVAFHEFGHAAAAASEGVKLEYIAVFIALLFPGALVAFNY 180

Query: 181 DALQDSSCFNALRIYCAGIWHNAVLSAASGLILFFLPLILFPLYIHGESPMVLNVPFTSP 240
           DALQDSSCFNALRIYCAGIWHN  LSAASGLILFFLPLILFPLYIHG+SP VL+VP+TSP
Sbjct: 181 DALQDSSCFNALRIYCAGIWHNTALSAASGLILFFLPLILFPLYIHGQSPTVLDVPYTSP 240

Query: 241 LSGYLSHGHVILSLDGIHVRSVDDWINLSTQISELTFQNETLSRLGENNRMANGRKEYCV 300
           LS YLSHGHVILSLDG+HV SVDDWINLS QIS+LTFQNET SRL ENN+MANGR+ YC 
Sbjct: 241 LSSYLSHGHVILSLDGMHVHSVDDWINLSAQISDLTFQNETHSRLVENNQMANGRRGYCF 300

Query: 301 PNFMLKESNKVQFTHDRLACFGDLTSFTSIPCVSSTALIDGDVEDSHSNRKERIYCLNPS 360
           PNFMLKESNKVQFTHD+  CFGD TSFTSIPCVSS  LIDG  ED++SNRKE IYCLN +
Sbjct: 301 PNFMLKESNKVQFTHDQSTCFGDFTSFTSIPCVSSAGLIDGYTEDNYSNRKEGIYCLNVN 360

Query: 361 DVIKLDKCTRQWDKSISNDSNSTCMCSQDETCLSPVHVPGLVWVEITYLNPHSSDCFYSR 420
           DV+KL+KC+  WDK+  ND+ S+CMCSQDETCLSPV +PGLVWVEITYLNP+SSDC YSR
Sbjct: 361 DVMKLNKCS-SWDKAAINDNTSSCMCSQDETCLSPVQMPGLVWVEITYLNPYSSDCSYSR 420

Query: 421 EYLLPSSNCSGTFIFVGDVVSMARSIQLTMYRPRLDFHFATYLPDVLERILLCLFHASLA 480
           EY LPSSNCSGTFIFVGDVVSMARSIQLTMYRPRLDFHFA YLPDVLE+IL CLFH SLA
Sbjct: 421 EYPLPSSNCSGTFIFVGDVVSMARSIQLTMYRPRLDFHFAIYLPDVLEKILSCLFHTSLA 480

Query: 481 LALLNSFPVYYLDGESILEIIIFQLTSMSPRNKEKVLRSFLMGGTLMSTFLLLRIFFHLF 540
           LALLNS PVY LDGESILEIIIFQLTS+SPRNKEKVLRSFLMGGTL+S FLLLRIFFHL 
Sbjct: 481 LALLNSLPVYCLDGESILEIIIFQLTSLSPRNKEKVLRSFLMGGTLISIFLLLRIFFHLL 540

Query: 541 VS 543
           VS
Sbjct: 541 VS 541

BLAST of Tan0006864 vs. ExPASy TrEMBL
Match: A0A6J1H2Q0 (Endopeptidase S2P OS=Cucurbita moschata OX=3662 GN=LOC111459160 PE=4 SV=1)

HSP 1 Score: 953.0 bits (2462), Expect = 5.5e-274
Identity = 473/542 (87.27%), Postives = 505/542 (93.17%), Query Frame = 0

Query: 1   MSGRWRRSKRPMAQADAHARIPPLLPLSTSRRGLSNAISCWYCDCKITSFNEPIFHFGRR 60
           MSGRWR SKRP A+ADAHAR+PPLLPLSTSR+GLSNA+SCWYCDCKITSFNEPIFHFGRR
Sbjct: 1   MSGRWRLSKRPRAEADAHARVPPLLPLSTSRKGLSNAVSCWYCDCKITSFNEPIFHFGRR 60

Query: 61  HARVLRAWFSTGIGFSLAALAAVTTVLFMELAIAMHIFGNNNVLRGLPISSSSLFGLPPL 120
           HARVLRAWFS GIGFSLAALA VTTVLF+ELAIAMHIFGN++V   LPIS SSLFGLPPL
Sbjct: 61  HARVLRAWFSIGIGFSLAALAVVTTVLFLELAIAMHIFGNSDVPHSLPISYSSLFGLPPL 120

Query: 121 ISSSSFAPADAGYIIISTLISVAFHEFGHAAAAASEGIKLEYVAVFIALLFPGALVAFNH 180
           ISS +F+PADAGYIIIS+LISVAFHEFGHAAA ASEGIKLEYVAVFIALLFPGALVAFNH
Sbjct: 121 ISSCNFSPADAGYIIISSLISVAFHEFGHAAAVASEGIKLEYVAVFIALLFPGALVAFNH 180

Query: 181 DALQDSSCFNALRIYCAGIWHNAVLSAASGLILFFLPLILFPLYIHGESPMVLNVPFTSP 240
           D LQDSSCFNALRIYCAGIWHNAVLSAASGL+LF LPLILFPLYIHGESPMVL+VP TSP
Sbjct: 181 DVLQDSSCFNALRIYCAGIWHNAVLSAASGLMLFCLPLILFPLYIHGESPMVLDVPSTSP 240

Query: 241 LSGYLSHGHVILSLDGIHVRSVDDWINLSTQISELTFQNETLSRLGENNRMANGRKEYCV 300
           LSGYLSHGH+ILSLDG+H+++VDDW+NLS QISE TFQN TLSRLGEN+RMANGRK YCV
Sbjct: 241 LSGYLSHGHLILSLDGMHIQNVDDWVNLSAQISESTFQNGTLSRLGENDRMANGRKGYCV 300

Query: 301 PNFMLKESNKVQFTHDRLACFGDLTSFTSIPCVSSTALIDGDVEDSHSNRKERIYCLNPS 360
           PNFMLKESNKVQF+HD+  CFGDLTSFTSIPCVSST L+DGDV+DSH NRKE I+CLN +
Sbjct: 301 PNFMLKESNKVQFSHDQSTCFGDLTSFTSIPCVSSTVLVDGDVDDSHYNRKEGIFCLNVN 360

Query: 361 DVIKLDKCTRQWDKSISNDSNSTCMCSQDETCLSPVHVPGLVWVEITYLNPHSSDCFYSR 420
           D+IKL+KC   WDK+I NDS STCMCSQDETCLSPV +PG VWVEITYLNPHSSDCFYSR
Sbjct: 361 DIIKLNKCISGWDKAIINDSTSTCMCSQDETCLSPVQMPGSVWVEITYLNPHSSDCFYSR 420

Query: 421 EYLLPSSNCSGTFIFVGDVVSMARSIQLTMYRPRLDFHFATYLPDVLERILLCLFHASLA 480
           E  LPSSNCSGTFIFVGDVVSMA SIQLTMYRPRLDFH+A YLPDVLERI LCLFHASLA
Sbjct: 421 ENPLPSSNCSGTFIFVGDVVSMAHSIQLTMYRPRLDFHYARYLPDVLERIFLCLFHASLA 480

Query: 481 LALLNSFPVYYLDGESILEIIIFQLTSMSPRNKEKVLRSFLMGGTLMSTFLLLRIFFHLF 540
           LALLNS PVYYLDGESILEIIIFQLTSMSPRNKEKVLR+FLMGGTLMS FLLLRIFFH+F
Sbjct: 481 LALLNSLPVYYLDGESILEIIIFQLTSMSPRNKEKVLRAFLMGGTLMSIFLLLRIFFHVF 540

Query: 541 VS 543
           VS
Sbjct: 541 VS 542

BLAST of Tan0006864 vs. ExPASy TrEMBL
Match: A0A6J1JYM6 (Endopeptidase S2P OS=Cucurbita maxima OX=3661 GN=LOC111490932 PE=4 SV=1)

HSP 1 Score: 944.9 bits (2441), Expect = 1.5e-271
Identity = 469/542 (86.53%), Postives = 503/542 (92.80%), Query Frame = 0

Query: 1   MSGRWRRSKRPMAQADAHARIPPLLPLSTSRRGLSNAISCWYCDCKITSFNEPIFHFGRR 60
           M GRWR SKRP A+ADA+AR+PPLLPLSTSR+GLSNA+SCWYCDCKITSFNEPIFHFGRR
Sbjct: 1   MPGRWRLSKRPRAEADANARVPPLLPLSTSRKGLSNAVSCWYCDCKITSFNEPIFHFGRR 60

Query: 61  HARVLRAWFSTGIGFSLAALAAVTTVLFMELAIAMHIFGNNNVLRGLPISSSSLFGLPPL 120
           HARVLRAWFS GIGFSLAALA VTTVLF+ELAI MHIFGN++V   LPIS SSLFGLPPL
Sbjct: 61  HARVLRAWFSIGIGFSLAALAVVTTVLFLELAITMHIFGNSDVPHSLPISYSSLFGLPPL 120

Query: 121 ISSSSFAPADAGYIIISTLISVAFHEFGHAAAAASEGIKLEYVAVFIALLFPGALVAFNH 180
           ISS +FAPADAGYIIIS+LISVAFHEFGHAAA ASEGIKLEYVAVFIALLFPGALVAFNH
Sbjct: 121 ISSCNFAPADAGYIIISSLISVAFHEFGHAAAVASEGIKLEYVAVFIALLFPGALVAFNH 180

Query: 181 DALQDSSCFNALRIYCAGIWHNAVLSAASGLILFFLPLILFPLYIHGESPMVLNVPFTSP 240
           D LQDSSCFNALRIYCAGIWHNAVLSAASGL+LF LPLILFPLYIHGESPMVL+VP TSP
Sbjct: 181 DVLQDSSCFNALRIYCAGIWHNAVLSAASGLMLFCLPLILFPLYIHGESPMVLDVPSTSP 240

Query: 241 LSGYLSHGHVILSLDGIHVRSVDDWINLSTQISELTFQNETLSRLGENNRMANGRKEYCV 300
           LSGYLSHGH+ILSLDG+H+++VDDW+NLS QISE TFQN TLSRLGEN++MANGRK YCV
Sbjct: 241 LSGYLSHGHLILSLDGMHIQNVDDWVNLSVQISESTFQNGTLSRLGENDQMANGRKGYCV 300

Query: 301 PNFMLKESNKVQFTHDRLACFGDLTSFTSIPCVSSTALIDGDVEDSHSNRKERIYCLNPS 360
           PNFMLKESNKVQF+HD+  CFGDLTSFTSIPCVSST L+DGDV+DSH NRKE I+CLN +
Sbjct: 301 PNFMLKESNKVQFSHDQSTCFGDLTSFTSIPCVSSTVLVDGDVDDSHYNRKEGIFCLNVN 360

Query: 361 DVIKLDKCTRQWDKSISNDSNSTCMCSQDETCLSPVHVPGLVWVEITYLNPHSSDCFYSR 420
           DVIKL+KC   WDK+I NDS STCMCSQDETCLSPV +PG +WVEITYLNPHSSDCFYSR
Sbjct: 361 DVIKLNKCISGWDKAIINDSTSTCMCSQDETCLSPVQMPGSIWVEITYLNPHSSDCFYSR 420

Query: 421 EYLLPSSNCSGTFIFVGDVVSMARSIQLTMYRPRLDFHFATYLPDVLERILLCLFHASLA 480
           E  LPSSNCSGTFIFVGDVVSMA SIQLTMY+PRLDFH+A YLPDVLERI LCLFHASLA
Sbjct: 421 ENPLPSSNCSGTFIFVGDVVSMAHSIQLTMYQPRLDFHYARYLPDVLERIFLCLFHASLA 480

Query: 481 LALLNSFPVYYLDGESILEIIIFQLTSMSPRNKEKVLRSFLMGGTLMSTFLLLRIFFHLF 540
           LALLNS PVYYLDGESILEIIIFQLTSMSPRNKEKVLR+FLMGGTLMS FLLLRIFFH+F
Sbjct: 481 LALLNSLPVYYLDGESILEIIIFQLTSMSPRNKEKVLRAFLMGGTLMSIFLLLRIFFHVF 540

Query: 541 VS 543
           VS
Sbjct: 541 VS 542

BLAST of Tan0006864 vs. ExPASy TrEMBL
Match: A0A0A0KQ25 (Endopeptidase S2P OS=Cucumis sativus OX=3659 GN=Csa_5G139060 PE=4 SV=1)

HSP 1 Score: 906.4 bits (2341), Expect = 5.9e-260
Identity = 457/542 (84.32%), Postives = 488/542 (90.04%), Query Frame = 0

Query: 1   MSGRWRRSKRPMAQADAHARIPPLLPLSTSRRGLSNAISCWYCDCKITSFNEPIFHFGRR 60
           M GRWRRSKR MAQADAHA IPPLLPL+TSR+GLSN+ISCWYCD KITSFNE IF FGRR
Sbjct: 1   MPGRWRRSKRLMAQADAHAHIPPLLPLTTSRKGLSNSISCWYCDYKITSFNELIFQFGRR 60

Query: 61  HARVLRAWFSTGIGFSLAALAAVTTVLFMELAIAMHIFGNNNVLRGLPISSSSLFGLPPL 120
           HARVLR WFS GIGFSLAALA V TVLF EL I MHIFG +NV+RGLP+S SSLFGLP L
Sbjct: 61  HARVLRTWFSIGIGFSLAALAVVATVLFRELTIVMHIFGKSNVIRGLPVSCSSLFGLPSL 120

Query: 121 ISSSSFAPADAGYIIISTLISVAFHEFGHAAAAASEGIKLEYVAVFIALLFPGALVAFNH 180
           ISS S  PA AGYIIISTLISVAFHEFGHAAAAASEG+KLEY+AVFIALLFPGALVAFN+
Sbjct: 121 ISSCSLFPAGAGYIIISTLISVAFHEFGHAAAAASEGVKLEYIAVFIALLFPGALVAFNY 180

Query: 181 DALQDSSCFNALRIYCAGIWHNAVLSAASGLILFFLPLILFPLYIHGESPMVLNVPFTSP 240
           DALQDSSCFNALRIYCAGIWHN  LSAASGLILFFLPLILFPLYIHG+SP VL+VP+TSP
Sbjct: 181 DALQDSSCFNALRIYCAGIWHNTALSAASGLILFFLPLILFPLYIHGQSPTVLDVPYTSP 240

Query: 241 LSGYLSHGHVILSLDGIHVRSVDDWINLSTQISELTFQNETLSRLGENNRMANGRKEYCV 300
           LS YLSHGHVILSLDG+HV SVDDWINLS QIS+LTFQNET SRL ENN+MANGR+ YC 
Sbjct: 241 LSSYLSHGHVILSLDGMHVHSVDDWINLSAQISDLTFQNETHSRLVENNQMANGRRGYCF 300

Query: 301 PNFMLKESNKVQFTHDRLACFGDLTSFTSIPCVSSTALIDGDVEDSHSNRKERIYCLNPS 360
           PNFMLKESNKVQFTHD+  CFGD TSFTSIPCVSS  LIDG  ED++SNRKE IYCLN +
Sbjct: 301 PNFMLKESNKVQFTHDQSTCFGDFTSFTSIPCVSSAGLIDGYTEDNYSNRKEGIYCLNVN 360

Query: 361 DVIKLDKCTRQWDKSISNDSNSTCMCSQDETCLSPVHVPGLVWVEITYLNPHSSDCFYSR 420
           DV+KL+KC+  WDK+  ND+ S+CMCSQDETCLSPV +PGLVWVEITYLNP+SSDC YSR
Sbjct: 361 DVMKLNKCS-SWDKAAINDNTSSCMCSQDETCLSPVQMPGLVWVEITYLNPYSSDCSYSR 420

Query: 421 EYLLPSSNCSGTFIFVGDVVSMARSIQLTMYRPRLDFHFATYLPDVLERILLCLFHASLA 480
           EY LPSSNCSGTFIFVGDVVSMARSIQLTMYRPRLDFHFA YLPDVLE+IL CLFH SLA
Sbjct: 421 EYPLPSSNCSGTFIFVGDVVSMARSIQLTMYRPRLDFHFAIYLPDVLEKILSCLFHTSLA 480

Query: 481 LALLNSFPVYYLDGESILEIIIFQLTSMSPRNKEKVLRSFLMGGTLMSTFLLLRIFFHLF 540
           LALLNS PVY LDGESILEIIIFQLTS+SPRNKEKVLRSFLMGGTL+S FLLLRIFFHL 
Sbjct: 481 LALLNSLPVYCLDGESILEIIIFQLTSLSPRNKEKVLRSFLMGGTLISIFLLLRIFFHLL 540

Query: 541 VS 543
           VS
Sbjct: 541 VS 541

BLAST of Tan0006864 vs. ExPASy TrEMBL
Match: A0A1S3AV46 (Endopeptidase S2P OS=Cucumis melo OX=3656 GN=LOC103482997 PE=4 SV=1)

HSP 1 Score: 904.8 bits (2337), Expect = 1.7e-259
Identity = 454/542 (83.76%), Postives = 487/542 (89.85%), Query Frame = 0

Query: 1   MSGRWRRSKRPMAQADAHARIPPLLPLSTSRRGLSNAISCWYCDCKITSFNEPIFHFGRR 60
           M GRWRRSKR MAQ D HA IP LLPL+TSR+G+SNAISCWYCD KITSFNE IF FGRR
Sbjct: 1   MPGRWRRSKRSMAQVDVHAHIPSLLPLTTSRKGISNAISCWYCDYKITSFNELIFQFGRR 60

Query: 61  HARVLRAWFSTGIGFSLAALAAVTTVLFMELAIAMHIFGNNNVLRGLPISSSSLFGLPPL 120
           HAR LR WFS GIGFSLAALA V TVLF E  I MHIF N+NV+RGLPIS SSLFGLP L
Sbjct: 61  HARALRTWFSIGIGFSLAALAVVATVLFRETKIIMHIFVNSNVIRGLPISCSSLFGLPSL 120

Query: 121 ISSSSFAPADAGYIIISTLISVAFHEFGHAAAAASEGIKLEYVAVFIALLFPGALVAFNH 180
           ISS SF PA AGYIIISTLISVAFHEFGHAAAAASEG+KLEYVAVFIALLFPGALVAFNH
Sbjct: 121 ISSCSFFPAGAGYIIISTLISVAFHEFGHAAAAASEGVKLEYVAVFIALLFPGALVAFNH 180

Query: 181 DALQDSSCFNALRIYCAGIWHNAVLSAASGLILFFLPLILFPLYIHGESPMVLNVPFTSP 240
           DALQDSSCF+ALRIYCAGIWHN  LSAASGLILFFLPLILFPLYIHG+SPMVL+VP+TSP
Sbjct: 181 DALQDSSCFSALRIYCAGIWHNTALSAASGLILFFLPLILFPLYIHGQSPMVLDVPYTSP 240

Query: 241 LSGYLSHGHVILSLDGIHVRSVDDWINLSTQISELTFQNETLSRLGENNRMANGRKEYCV 300
           LS YLSHGHVILSLDG+HV SVDDWINLS +ISELTFQNET SRLGENN MANGR+ YC 
Sbjct: 241 LSSYLSHGHVILSLDGMHVHSVDDWINLSAEISELTFQNETHSRLGENNLMANGRRGYCF 300

Query: 301 PNFMLKESNKVQFTHDRLACFGDLTSFTSIPCVSSTALIDGDVEDSHSNRKERIYCLNPS 360
           PNFMLKESNKVQFTHD+  CFGD TSFTSIPCVSST LIDG  ED++SNRKE IYCLN +
Sbjct: 301 PNFMLKESNKVQFTHDQATCFGDFTSFTSIPCVSSTGLIDGYTEDNNSNRKEGIYCLNVN 360

Query: 361 DVIKLDKCTRQWDKSISNDSNSTCMCSQDETCLSPVHVPGLVWVEITYLNPHSSDCFYSR 420
           DV+KL+KC+  WDK++ ND+ S+CMCS+DETCLSPV +PGLVWVEITYLNP+SSDCFYSR
Sbjct: 361 DVMKLNKCS-SWDKAVINDNTSSCMCSRDETCLSPVQMPGLVWVEITYLNPYSSDCFYSR 420

Query: 421 EYLLPSSNCSGTFIFVGDVVSMARSIQLTMYRPRLDFHFATYLPDVLERILLCLFHASLA 480
           EY LPSSNCSGTFIFVGDVVSMARSIQLTMYRPR DFHFATYLPDVLE+IL CLFH SLA
Sbjct: 421 EYPLPSSNCSGTFIFVGDVVSMARSIQLTMYRPRFDFHFATYLPDVLEKILSCLFHTSLA 480

Query: 481 LALLNSFPVYYLDGESILEIIIFQLTSMSPRNKEKVLRSFLMGGTLMSTFLLLRIFFHLF 540
           LALLNS PVY LDGESILEI+IFQLTS+SPRNKEKVLRSFL+ GTL+S FLLLRIFFHL 
Sbjct: 481 LALLNSLPVYCLDGESILEILIFQLTSLSPRNKEKVLRSFLIAGTLISIFLLLRIFFHLL 540

Query: 541 VS 543
           +S
Sbjct: 541 IS 541

BLAST of Tan0006864 vs. ExPASy TrEMBL
Match: A0A1S3AUN7 (Endopeptidase S2P OS=Cucumis melo OX=3656 GN=LOC103482997 PE=4 SV=1)

HSP 1 Score: 892.5 bits (2305), Expect = 8.8e-256
Identity = 451/542 (83.21%), Postives = 484/542 (89.30%), Query Frame = 0

Query: 1   MSGRWRRSKRPMAQADAHARIPPLLPLSTSRRGLSNAISCWYCDCKITSFNEPIFHFGRR 60
           M GRWRRSKR MAQ D HA IP LLPL+TSR+G+SNAISCWYCD KITSFNE IF FGR 
Sbjct: 1   MPGRWRRSKRSMAQVDVHAHIPSLLPLTTSRKGISNAISCWYCDYKITSFNELIFQFGR- 60

Query: 61  HARVLRAWFSTGIGFSLAALAAVTTVLFMELAIAMHIFGNNNVLRGLPISSSSLFGLPPL 120
             R LR WFS GIGFSLAALA V TVLF E  I MHIF N+NV+RGLPIS SSLFGLP L
Sbjct: 61  --RALRTWFSIGIGFSLAALAVVATVLFRETKIIMHIFVNSNVIRGLPISCSSLFGLPSL 120

Query: 121 ISSSSFAPADAGYIIISTLISVAFHEFGHAAAAASEGIKLEYVAVFIALLFPGALVAFNH 180
           ISS SF PA AGYIIISTLISVAFHEFGHAAAAASEG+KLEYVAVFIALLFPGALVAFNH
Sbjct: 121 ISSCSFFPAGAGYIIISTLISVAFHEFGHAAAAASEGVKLEYVAVFIALLFPGALVAFNH 180

Query: 181 DALQDSSCFNALRIYCAGIWHNAVLSAASGLILFFLPLILFPLYIHGESPMVLNVPFTSP 240
           DALQDSSCF+ALRIYCAGIWHN  LSAASGLILFFLPLILFPLYIHG+SPMVL+VP+TSP
Sbjct: 181 DALQDSSCFSALRIYCAGIWHNTALSAASGLILFFLPLILFPLYIHGQSPMVLDVPYTSP 240

Query: 241 LSGYLSHGHVILSLDGIHVRSVDDWINLSTQISELTFQNETLSRLGENNRMANGRKEYCV 300
           LS YLSHGHVILSLDG+HV SVDDWINLS +ISELTFQNET SRLGENN MANGR+ YC 
Sbjct: 241 LSSYLSHGHVILSLDGMHVHSVDDWINLSAEISELTFQNETHSRLGENNLMANGRRGYCF 300

Query: 301 PNFMLKESNKVQFTHDRLACFGDLTSFTSIPCVSSTALIDGDVEDSHSNRKERIYCLNPS 360
           PNFMLKESNKVQFTHD+  CFGD TSFTSIPCVSST LIDG  ED++SNRKE IYCLN +
Sbjct: 301 PNFMLKESNKVQFTHDQATCFGDFTSFTSIPCVSSTGLIDGYTEDNNSNRKEGIYCLNVN 360

Query: 361 DVIKLDKCTRQWDKSISNDSNSTCMCSQDETCLSPVHVPGLVWVEITYLNPHSSDCFYSR 420
           DV+KL+KC+  WDK++ ND+ S+CMCS+DETCLSPV +PGLVWVEITYLNP+SSDCFYSR
Sbjct: 361 DVMKLNKCS-SWDKAVINDNTSSCMCSRDETCLSPVQMPGLVWVEITYLNPYSSDCFYSR 420

Query: 421 EYLLPSSNCSGTFIFVGDVVSMARSIQLTMYRPRLDFHFATYLPDVLERILLCLFHASLA 480
           EY LPSSNCSGTFIFVGDVVSMARSIQLTMYRPR DFHFATYLPDVLE+IL CLFH SLA
Sbjct: 421 EYPLPSSNCSGTFIFVGDVVSMARSIQLTMYRPRFDFHFATYLPDVLEKILSCLFHTSLA 480

Query: 481 LALLNSFPVYYLDGESILEIIIFQLTSMSPRNKEKVLRSFLMGGTLMSTFLLLRIFFHLF 540
           LALLNS PVY LDGESILEI+IFQLTS+SPRNKEKVLRSFL+ GTL+S FLLLRIFFHL 
Sbjct: 481 LALLNSLPVYCLDGESILEILIFQLTSLSPRNKEKVLRSFLIAGTLISIFLLLRIFFHLL 538

Query: 541 VS 543
           +S
Sbjct: 541 IS 538

BLAST of Tan0006864 vs. TAIR 10
Match: AT4G20310.2 (Peptidase M50 family protein )

HSP 1 Score: 380.9 bits (977), Expect = 1.7e-105
Identity = 219/510 (42.94%), Postives = 297/510 (58.24%), Query Frame = 0

Query: 29  TSRRGLSNAISCWYCDCKITSFNEPIFHFGRRHARVLRAWFSTGIGFSLAALAAVTTVLF 88
           T    + N  SC YCD KI++FNEPIF  GRR + VL+ WFS G+GF +A+L  VT  L 
Sbjct: 21  TGGENIENEASCCYCDLKISNFNEPIFRLGRRFSGVLKVWFSIGLGFGVASLILVTVFLL 80

Query: 89  MELAIAMHIFGNNNVLRGLPISSSSLFGLPPLISSSSFAPADAGYIIISTLISVAFHEFG 148
           ++   +  +F N          +S++FG  P   S+  + +   Y+++ST+I+V+ HE G
Sbjct: 81  LQFH-SNPLFSNR--------LTSAVFGFSP---STRVSLSGIAYVLVSTVITVSVHELG 140

Query: 149 HAAAAASEGIKLEYVAVFIALLFPGALVAFNHDALQDSSCFNALRIYCAGIWHNAVLSAA 208
           HA AAASEGI++EY+AVFIA +FPG LVAF++D LQ    FNALRIYCAGIWHNAV  A 
Sbjct: 141 HALAAASEGIQMEYIAVFIAAIFPGGLVAFDNDVLQSLPSFNALRIYCAGIWHNAVFCAL 200

Query: 209 SGLILFFLPLILFPLYIHGESPMVLNVPFTSPLSGYLSHGHVILSLDGIHVRSVDDWINL 268
               LF LP++L P Y HGES  V++VP  SPL GYLS G VI+SLDGI V    +W+ L
Sbjct: 201 CVFALFLLPVMLSPFYKHGESLTVVDVPSVSPLFGYLSPGDVIVSLDGIQVHKPSEWLEL 260

Query: 269 STQISELTFQNETLS-RLGENNRMANGRKEYCVPNFMLKESNKVQFTHDRLACFGDLTSF 328
           +  + +   +    S  LG + R  +G K YCVP  +++E  K +   ++  C GDLT+F
Sbjct: 261 AAILDKENSKTSNGSLYLGGSRRFHHG-KGYCVPISLIEEGYKGKMVENQFVCPGDLTAF 320

Query: 329 TSIPCVSSTALIDGDVEDSHSNRKERIYCLNPSDVIKLDKCTRQWDKSISNDSNSTCMCS 388
            ++PC             S++  +E   CL+  D++KL KC   W  +   D+ S C+C 
Sbjct: 321 RTMPC-------------SNAAIREVSVCLDAKDIVKLQKCGDGWVTTSDTDNQSDCVCP 380

Query: 389 QDETCLSPVHVPGLVWVEITYLNPHSSDCFYSREYLLPSSNCSGTFIFVGDVVSMARSIQ 448
           Q + CL  +  PG++W EITY    S DC                          +R + 
Sbjct: 381 QGDLCLQAMQSPGVLWTEITYKRTSSQDC--------------------------SRLVH 440

Query: 449 LTMYRPRLDFH-FATYLPDVLERILLCLFHASLALALLNSFPVYYLDGESILEIIIFQLT 508
           LT Y+PR  F+ F    P++LER L C FH SLAL LLNS PVYYLDGESILE  +   T
Sbjct: 441 LTAYQPRWLFNFFGKSFPNILERSLTCTFHVSLALVLLNSLPVYYLDGESILESSLQSFT 478

Query: 509 SMSPRNKEKVLRSFLMGGTLMSTFLLLRIF 537
            +SPR K+K L+  L+GG+L+S     RIF
Sbjct: 501 WLSPRKKKKALQVCLVGGSLLSFLAFFRIF 478

BLAST of Tan0006864 vs. TAIR 10
Match: AT4G20310.3 (Peptidase M50 family protein )

HSP 1 Score: 320.9 bits (821), Expect = 2.0e-87
Identity = 178/380 (46.84%), Postives = 236/380 (62.11%), Query Frame = 0

Query: 160 LEYVAVFIALLFPGALVAFNHDALQDSSCFNALRIYCAGIWHNAVLSAASGLILFFLPLI 219
           +EY+AVFIA +FPG LVAF++D LQ    FNALRIYCAGIWHNAV  A     LF LP++
Sbjct: 1   MEYIAVFIAAIFPGGLVAFDNDVLQSLPSFNALRIYCAGIWHNAVFCALCVFALFLLPVM 60

Query: 220 LFPLYIHGESPMVLNVPFTSPLSGYLSHGHVILSLDGIHVRSVDDWINLSTQISELTFQN 279
           L P Y HGES  V++VP  SPL GYLS G VI+SLDGI V    +W+ L+  + +   + 
Sbjct: 61  LSPFYKHGESLTVVDVPSVSPLFGYLSPGDVIVSLDGIQVHKPSEWLELAAILDKENSKT 120

Query: 280 ETLS-RLGENNRMANGRKEYCVPNFMLKESNKVQFTHDRLACFGDLTSFTSIPCVSSTAL 339
              S  LG + R  +G K YCVP  +++E  K +   ++  C GDLT+F ++PC      
Sbjct: 121 SNGSLYLGGSRRFHHG-KGYCVPISLIEEGYKGKMVENQFVCPGDLTAFRTMPC------ 180

Query: 340 IDGDVEDSHSNRKERIYCLNPSDVIKLDKCTRQWDKSISNDSNSTCMCSQDETCLSPVHV 399
                  S++  +E   CL+  D++KL KC   W  +   D+ S C+C Q + CL  +  
Sbjct: 181 -------SNAAIREVSVCLDAKDIVKLQKCGDGWVTTSDTDNQSDCVCPQGDLCLQAMQS 240

Query: 400 PGLVWVEITYLNPHSSDCFYSREYL-LPSSNCSGTFIFVGDVVSMARSIQLTMYRPRLDF 459
           PG++W EITY    S DC  SR  L   +SNC GTF+FVGD+++M+ S+ LT Y+PR  F
Sbjct: 241 PGVLWTEITYKRTSSQDC--SRLGLDFNTSNCLGTFVFVGDLIAMSHSVHLTAYQPRWLF 300

Query: 460 H-FATYLPDVLERILLCLFHASLALALLNSFPVYYLDGESILEIIIFQLTSMSPRNKEKV 519
           + F    P++LER L C FH SLAL LLNS PVYYLDGESILE  +   T +SPR K+K 
Sbjct: 301 NFFGKSFPNILERSLTCTFHVSLALVLLNSLPVYYLDGESILESSLQSFTWLSPRKKKKA 360

Query: 520 LRSFLMGGTLMSTFLLLRIF 537
           L+  L+GG+L+S     RIF
Sbjct: 361 LQVCLVGGSLLSFLAFFRIF 364

BLAST of Tan0006864 vs. TAIR 10
Match: AT4G20310.1 (Peptidase M50 family protein )

HSP 1 Score: 279.6 bits (714), Expect = 5.2e-75
Identity = 156/361 (43.21%), Postives = 217/361 (60.11%), Query Frame = 0

Query: 29  TSRRGLSNAISCWYCDCKITSFNEPIFHFGRRHARVLRAWFSTGIGFSLAALAAVTTVLF 88
           T    + N  SC YCD KI++FNEPIF  GRR + VL+ WFS G+GF +A+L  VT  L 
Sbjct: 21  TGGENIENEASCCYCDLKISNFNEPIFRLGRRFSGVLKVWFSIGLGFGVASLILVTVFLL 80

Query: 89  MELAIAMHIFGNNNVLRGLPISSSSLFGLPPLISSSSFAPADAGYIIISTLISVAFHEFG 148
           ++   +  +F N          +S++FG  P   S+  + +   Y+++ST+I+V+ HE G
Sbjct: 81  LQFH-SNPLFSNR--------LTSAVFGFSP---STRVSLSGIAYVLVSTVITVSVHELG 140

Query: 149 HAAAAASEGIKLEYVAVFIALLFPGALVAFNHDALQDSSCFNALRIYCAGIWHNAVLSAA 208
           HA AAASEGI++EY+AVFIA +FPG LVAF++D LQ    FNALRIYCAGIWHNAV  A 
Sbjct: 141 HALAAASEGIQMEYIAVFIAAIFPGGLVAFDNDVLQSLPSFNALRIYCAGIWHNAVFCAL 200

Query: 209 SGLILFFLPLILFPLYIHGESPMVLNVPFTSPLSGYLSHGHVILSLDGIHVRSVDDWINL 268
               LF LP++L P Y HGES  V++VP  SPL GYLS G VI+SLDGI V    +W+ L
Sbjct: 201 CVFALFLLPVMLSPFYKHGESLTVVDVPSVSPLFGYLSPGDVIVSLDGIQVHKPSEWLEL 260

Query: 269 STQISELTFQNETLS-RLGENNRMANGRKEYCVPNFMLKESNKVQFTHDRLACFGDLTSF 328
           +  + +   +    S  LG + R  +G K YCVP  +++E  K +   ++  C GDLT+F
Sbjct: 261 AAILDKENSKTSNGSLYLGGSRRFHHG-KGYCVPISLIEEGYKGKMVENQFVCPGDLTAF 320

Query: 329 TSIPCVSSTALIDGDVEDSHSNRKERIYCLNPSDVIKLDKCTRQWDKSISNDSNSTCMCS 388
            ++PC             S++  +E   CL+  D++KL KC   W  +   D+ S C+C 
Sbjct: 321 RTMPC-------------SNAAIREVSVCLDAKDIVKLQKCGDGWVTTSDTDNQSDCVCP 355