Tan0006809 (gene) Snake gourd v1

Overview
NameTan0006809
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionPectinesterase
LocationLG09: 62550638 .. 62554221 (-)
RNA-Seq ExpressionTan0006809
SyntenyTan0006809
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTCGGCCTACCGTTGCTTCCTCTTTTCCGGCGACGGTAGCGATCGCATTGGCGGTGGTCCAACTGATCATCATAATTCCGTCCGCCGTCGTCGAAGCTCGCGCGAAGCCGATTCCGGGAGAGCCATCGAAGCTCGACGAATGGATCGCAAACAACATGAGAGAATACAACGATCGGAAGGCGAACGAGACGGGAGTCAAGGCGCTGGACCTGCGCCTGGCGGAGGCGGAGGACGGCGTTCGACTGCTGACCGTTAGAAAAGACGGAAGAGGAAATTTCACGACGATCACCGAGGCGATCGAGAGCATTCCCGTCGGAAATCGGCGGCGAGTGGTGGTGTGGATCGGCGGCGGAGTTTACCGGGAGAAGATTACGATCAAGGCTTCGAAGCCGTTCGTGACGCTGTACGGACAGAAAGGAAAACGGCCGATGATAATGTACGACGGCACGGCGTCGGAATTTGGTACCGTCGACAGCGCGACGGTGGCGGTGGAATCCGATTATTTCGTTGCCGTCAATGTTGCGTTTGTGGTGCGTTTTTCTTCTTCTTCTTCTTCCTTTTCTGTTTTTTTTGGGGGGCAAGTTTTGTAATTAGTGATAATTATGGGGGTTTTTAAACAAAAAAATCATGCAGTATCGAAATTATTACAAGCCGTAATTGGGGAAATTATCGAAATTATTAGGGCCGTACTTGGAAAAATGATTGGAGGAGGCGGGTAGTATGAGACTCCCCCAATTTGATATTAGTACATGGTTTTTTTTAAGTATAATATTAGTACACGGTTGGTTAATTATTGCTTAATCACCTTAGTTAATTTTTTTTTAAAAAAATTATTTTTCTCTTATATTTCAATTTTTTTATTTCAATTTTTTTTTGTTAATTTTCAATTGTCTATTTTTTTTATAATTATCTTAGAGTTGATTAAGATTCCGTAGTTTATTATTGTTATTATTCTTTTTCAATAATTTTCTTTTTTTTTTTTAAACTTTTATGTTCTTATCTTGTCTTTTTCTTTTATTCTCATTTTAAAATTTTGCTTGATATTTTCATATGAATTCAACAAAAAAAAAAATTTCTTATATTTATCCGTTCTTCCTTTTTATTTCTCTAAGATTCTTTATTCAGTTTGGGTATCATTGATTTTTAAACTATATTTTAATATGTTAAGCACGGTAACAATAATTTTACTTTTGATTTACACTTATCAAAACTCATCATATAATAATTTTGATGGAAGTATTTGGAGGGTAAAGGATGTTTACATAAATTTGTCTTAGGTAAAAAAATTTTATTTAATAATTAATATTATGCCCTCTAATATTTGTCATATTAATTTCAACTATTTTTTTTATTATGCACGAGGTAAGTTCTATTAGTTTATGTAATTTTGAAAATGATGTTGGACATTGATATTTCCTAGATGTGAATCTTTAATTCTATTTTTTCTATTCTTATTGATTTTTTTTACCCTAAATATTGCTAGCCAATATTATGTTTTCATGTTTCTTAAATTGAAACTTACTAGCTTGTATTGTTTTCATTTTTATTTCATATAGTTTCAAGAATCATACATGCAATCTAAAAATAAAAAATAAAATAAAAAACTGTTATGGTTAAGCAATAGTTAACTAATTGTACGTGCATCAAATTGGGAAAGAGTCTGTCAACAAGAGTATAGCTCAACTGTCATTAAGTATGGGTTACTCGGGTTATCGGATTATTTGTACACCGCTACCTCTTATGTACAAAACATGATTTTGCACGTAGATCATGTCTTGACCTTTTCTTGTACGATGGGTGACGTAGTTTTAATTTTTAAATTAATATTTTTAGGTTCTATTTAATAATCATTTATTTTTTAGTTTTTGGAAATTATAGTTGTTTTTTTCATAATTTTATATTCTCTACCATAATTTCTATTTTTTTTAGCTATCATTTAAGTTTTTAGGCTTAATTTTCAAAAACCAAATGGTTATCAAATAAGGCTTTAAGTCTTTGAGATTTAAATTTTGTTTCTATTTAGTCTTAAAAATGTTTTAAACGTGCGCGACTCCAAAATTTGAATTTGATTTCTATTAGTCCATAAAATTTGAAATTGGTTAATTGACCTAATAAAAATGATGACGTGGTAATTAACATATATGCTAAACTAGATTTTTTTTAGTACAATAGAAATTGATCAGAGATTCAAAACCCAGACCTTAGAGAACACATTCTGTAACGCCCCAGGGCCAGGATCATTCATGACTGCTTCTAAGAGCGAAAACACTATCCCCACAAACCAACACGGGGTCATTTCAGCATGTTTTGTCCTCACTCACATGCTCTCTAAGAAAATTCCCAAGAGGTCACCTACACTTAACTGTGGAGTTCCTATGATTGAGCCATCGAAAAGGAAAGTGCACATTGTTGGTATAGGTAGTAGCTATCAATTCTTTTAAGTCTTTCTCAACTGTACTTTCATATCTTCATGGTCATTCTCATGTACCCATTTTCCTCGGGCACCATACTTTTAATTTGCTCAGGATCCCTCTGCCCATTCAGATGTGGGATTCGGTTCCATTCAGGATCCCTCTCATTCATTTCAATAGAGACCAGTTGAGCTATATAGGCTTTGTTTGACCCTAGAAATGGAAGTGGGAATTTATTTTTGGTTTAAGTAATCAAATAAGAAAGCATTTATAGTAATGAATTAATAAATTAGTTAAAAATGAATTTATAAATTGAGATGTGTTAATGTGAAGATTTGTATGAGTGTACTGTGTGCAGAATTCAGCTCCAATGCCTGATGGTAGAGGAACAGGAGGACAAGCAGTGGCAATGAGGATATCTGGAGATAAGGCAGCATTTCATGAGTGCCATTTTATAGGGTTTCAAGACACATTATGTGATGATAGAGGCAGACATTTTTTCAAGGATTGTTACATCCAAGGCACTGTTGATTTCATCTTTGGAAATGGGAAATCCCTCTACTTGGTACATATATATATATATATAGTGTATAGAGTTATAGTGTGTAAGAGAAATTAAAAGGGTGTAATTATAATATTAATTGTTATATATATTATGACAGAAAACGAGTATAAAGTCAGTGGCGGAAGGGACAGGAGTGATCACAGCTCAAGCAAGGGAAGAAGCGAGAGACGAGAGTGGATTCACATTTGCATATTGTAACATCACAGGAACTGGAAACACTTATTTGGGAAGAGCTTGGAAAGAGAGAACTCGTGTTGTTTTTGCTTACACTTTTATGGGAAATCTCATCAACACTCAAGGATGGTCCGACAAAATGCATGGAACCCAACCTAGAAAGTAATTACATTACCTATATATATTCATTTTCAATACCCTCCTAAATACCAATTATTATTATTATTATTATTATAGGAGTATGTACTACGGTGAGTACAAATGCATGGGCCCCGGGGCCAGCCCCTCAGGACGAGCGCATTATGCCAGGATACTCTCCGACCAAGAAGCAAAGGCGTTTTTAAGTATGACTTATATCCAAGGAAACAAATGGCTTCTCCCACCTCCCGATCTCTCCCTCTCCCGCTCTCAACCCAATTCCTTCCATTGA

mRNA sequence

ATGTCTCGGCCTACCGTTGCTTCCTCTTTTCCGGCGACGGTAGCGATCGCATTGGCGGTGGTCCAACTGATCATCATAATTCCGTCCGCCGTCGTCGAAGCTCGCGCGAAGCCGATTCCGGGAGAGCCATCGAAGCTCGACGAATGGATCGCAAACAACATGAGAGAATACAACGATCGGAAGGCGAACGAGACGGGAGTCAAGGCGCTGGACCTGCGCCTGGCGGAGGCGGAGGACGGCGTTCGACTGCTGACCGTTAGAAAAGACGGAAGAGGAAATTTCACGACGATCACCGAGGCGATCGAGAGCATTCCCGTCGGAAATCGGCGGCGAGTGGTGGTGTGGATCGGCGGCGGAGTTTACCGGGAGAAGATTACGATCAAGGCTTCGAAGCCGTTCGTGACGCTGTACGGACAGAAAGGAAAACGGCCGATGATAATGTACGACGGCACGGCGTCGGAATTTGGTACCGTCGACAGCGCGACGGTGGCGGTGGAATCCGATTATTTCGTTGCCGTCAATGTTGCGTTTGTGAATTCAGCTCCAATGCCTGATGGTAGAGGAACAGGAGGACAAGCAGTGGCAATGAGGATATCTGGAGATAAGGCAGCATTTCATGAGTGCCATTTTATAGGGTTTCAAGACACATTATGTGATGATAGAGGCAGACATTTTTTCAAGGATTGTTACATCCAAGGCACTGTTGATTTCATCTTTGGAAATGGGAAATCCCTCTACTTGAAAACGAGTATAAAGTCAGTGGCGGAAGGGACAGGAGTGATCACAGCTCAAGCAAGGGAAGAAGCGAGAGACGAGAGTGGATTCACATTTGCATATTGTAACATCACAGGAACTGGAAACACTTATTTGGGAAGAGCTTGGAAAGAGAGAACTCGTGTTGTTTTTGCTTACACTTTTATGGGAAATCTCATCAACACTCAAGGATGGTCCGACAAAATGCATGGAACCCAACCTAGAAAGAGTATGTACTACGGTGAGTACAAATGCATGGGCCCCGGGGCCAGCCCCTCAGGACGAGCGCATTATGCCAGGATACTCTCCGACCAAGAAGCAAAGGCGTTTTTAAGTATGACTTATATCCAAGGAAACAAATGGCTTCTCCCACCTCCCGATCTCTCCCTCTCCCGCTCTCAACCCAATTCCTTCCATTGA

Coding sequence (CDS)

ATGTCTCGGCCTACCGTTGCTTCCTCTTTTCCGGCGACGGTAGCGATCGCATTGGCGGTGGTCCAACTGATCATCATAATTCCGTCCGCCGTCGTCGAAGCTCGCGCGAAGCCGATTCCGGGAGAGCCATCGAAGCTCGACGAATGGATCGCAAACAACATGAGAGAATACAACGATCGGAAGGCGAACGAGACGGGAGTCAAGGCGCTGGACCTGCGCCTGGCGGAGGCGGAGGACGGCGTTCGACTGCTGACCGTTAGAAAAGACGGAAGAGGAAATTTCACGACGATCACCGAGGCGATCGAGAGCATTCCCGTCGGAAATCGGCGGCGAGTGGTGGTGTGGATCGGCGGCGGAGTTTACCGGGAGAAGATTACGATCAAGGCTTCGAAGCCGTTCGTGACGCTGTACGGACAGAAAGGAAAACGGCCGATGATAATGTACGACGGCACGGCGTCGGAATTTGGTACCGTCGACAGCGCGACGGTGGCGGTGGAATCCGATTATTTCGTTGCCGTCAATGTTGCGTTTGTGAATTCAGCTCCAATGCCTGATGGTAGAGGAACAGGAGGACAAGCAGTGGCAATGAGGATATCTGGAGATAAGGCAGCATTTCATGAGTGCCATTTTATAGGGTTTCAAGACACATTATGTGATGATAGAGGCAGACATTTTTTCAAGGATTGTTACATCCAAGGCACTGTTGATTTCATCTTTGGAAATGGGAAATCCCTCTACTTGAAAACGAGTATAAAGTCAGTGGCGGAAGGGACAGGAGTGATCACAGCTCAAGCAAGGGAAGAAGCGAGAGACGAGAGTGGATTCACATTTGCATATTGTAACATCACAGGAACTGGAAACACTTATTTGGGAAGAGCTTGGAAAGAGAGAACTCGTGTTGTTTTTGCTTACACTTTTATGGGAAATCTCATCAACACTCAAGGATGGTCCGACAAAATGCATGGAACCCAACCTAGAAAGAGTATGTACTACGGTGAGTACAAATGCATGGGCCCCGGGGCCAGCCCCTCAGGACGAGCGCATTATGCCAGGATACTCTCCGACCAAGAAGCAAAGGCGTTTTTAAGTATGACTTATATCCAAGGAAACAAATGGCTTCTCCCACCTCCCGATCTCTCCCTCTCCCGCTCTCAACCCAATTCCTTCCATTGA

Protein sequence

MSRPTVASSFPATVAIALAVVQLIIIIPSAVVEARAKPIPGEPSKLDEWIANNMREYNDRKANETGVKALDLRLAEAEDGVRLLTVRKDGRGNFTTITEAIESIPVGNRRRVVVWIGGGVYREKITIKASKPFVTLYGQKGKRPMIMYDGTASEFGTVDSATVAVESDYFVAVNVAFVNSAPMPDGRGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIFGNGKSLYLKTSIKSVAEGTGVITAQAREEARDESGFTFAYCNITGTGNTYLGRAWKERTRVVFAYTFMGNLINTQGWSDKMHGTQPRKSMYYGEYKCMGPGASPSGRAHYARILSDQEAKAFLSMTYIQGNKWLLPPPDLSLSRSQPNSFH
Homology
BLAST of Tan0006809 vs. ExPASy Swiss-Prot
Match: Q9FKF3 (Putative pectinesterase 63 OS=Arabidopsis thaliana OX=3702 GN=PME63 PE=3 SV=2)

HSP 1 Score: 356.7 bits (914), Expect = 3.4e-97
Identity = 175/364 (48.08%), Postives = 239/364 (65.66%), Query Frame = 0

Query: 16  IALAVVQLIIIIPSAVVEAR-AKPIPGEPSKLDEWIANNMREYNDRKANETGVKALDLRL 75
           ++L V  L+++I S VV    A PIP    ++++W   N                     
Sbjct: 6   VSLIVTILLVVITSPVVFGNDAAPIPENKGRIEQWFNTN--------------------- 65

Query: 76  AEAEDGVRLLTVRKDGRGNFTTITEAIESIPVGNRRRVVVWIGGGVYREKITIKASKPFV 135
                      V+++GRG+F TITEAI S+  GN RRV++ IG GVY+EK+TI  SKPF+
Sbjct: 66  -----------VKQNGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFI 125

Query: 136 TLYGQKGKRPMIMYDGTASEFGTVDSATVAVESDYFVAVNVAFVNSAPMPDGRGTGGQAV 195
           TLYG     P++ +DGTA+++GTVDSAT+ V SDYF+AVN+   NSAPMPDG+  G QA+
Sbjct: 126 TLYGHPNAMPVLTFDGTAAQYGTVDSATLIVLSDYFMAVNIILKNSAPMPDGKRKGAQAL 185

Query: 196 AMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIFGNGKSLYLKTSIKSV 255
           +MRISG+KAAF+ C F G+QDT+CDD G HFFKDCYI+GT DFIFG+G+SLYL T +  V
Sbjct: 186 SMRISGNKAAFYNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSLYLGTQLNVV 245

Query: 256 AEGTGVITAQAREEARDESGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTFMGNLINT 315
            +G  VITA A + A ++SG++F +C +TGTG   YLGR+W    +VV+AYT M +++N 
Sbjct: 246 GDGIRVITAHAGKSAAEKSGYSFVHCKVTGTGTGIYLGRSWMSHPKVVYAYTDMSSVVNP 305

Query: 316 QGWSDKMHGTQPRKSMYYGEYKCMGPGASPSGRAHYARILSDQEAKAFLSMTYIQGNKWL 375
            GW +     +  K+++YGEYKC G G+    R  Y + + D EAK F+S+ YIQG+ WL
Sbjct: 306 SGWQENREAGRD-KTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFISLGYIQGSSWL 336

Query: 376 LPPP 378
           LPPP
Sbjct: 366 LPPP 336

BLAST of Tan0006809 vs. ExPASy Swiss-Prot
Match: Q9LY17 (Probable pectinesterase 50 OS=Arabidopsis thaliana OX=3702 GN=PME50 PE=2 SV=1)

HSP 1 Score: 348.2 bits (892), Expect = 1.2e-94
Identity = 169/364 (46.43%), Postives = 244/364 (67.03%), Query Frame = 0

Query: 16  IALAVVQLIIIIPSAVVEAR-AKPIPGEPSKLDEWIANNMREYNDRKANETGVKALDLRL 75
           I+++VV  +++  S VV A    PIP   +++ +W   N++ Y+ RK        LD  L
Sbjct: 4   ISMSVVAFLVVFASPVVLATDTDPIPENRAQIPQWFKTNVKPYSQRKG------TLDPAL 63

Query: 76  AEAEDGVRLLTVRKDGRGNFTTITEAIESIPVGNRRRVVVWIGGGVYREKITIKASKPFV 135
             AE   +++TV + G  NF T+ EAI+SIP GN+ RV++ +  GVY EK+TI  ++PF+
Sbjct: 64  EAAEAARQIITVNQKGGANFKTLNEAIKSIPTGNKNRVIIKLAPGVYNEKVTIDIARPFI 123

Query: 136 TLYGQKGKRPMIMYDGTASEFGTVDSATVAVESDYFVAVNVAFVNSAPMPDGRGTGGQAV 195
           TL GQ G   ++ Y GTA+++GTV+SAT+ V ++YF A ++   N+APMP   G+ GQA+
Sbjct: 124 TLLGQPGAETVLTYHGTAAQYGTVESATLIVWAEYFQAAHLTIKNTAPMPK-PGSQGQAL 183

Query: 196 AMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIFGNGKSLYLKTSIKSV 255
           AMRI+ DKAAF+ C F GFQDTLCDD+G HFFKDCYI+GT DFIFG G SLYL T + +V
Sbjct: 184 AMRINADKAAFYSCRFHGFQDTLCDDKGNHFFKDCYIEGTYDFIFGRGASLYLNTQLHAV 243

Query: 256 AEGTGVITAQAREEARDESGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTFMGNLINT 315
            +G  VITAQ R+ A +++G+TF +C +TGTG   YLGR+W    +VV+A+T M +++N 
Sbjct: 244 GDGLRVITAQGRQSATEQNGYTFVHCKVTGTGTGIYLGRSWMSHPKVVYAFTEMTSVVNP 303

Query: 316 QGWSDKMHGTQPRKSMYYGEYKCMGPGASPSGRAHYARILSDQEAKAFLSMTYIQGNKWL 375
            GW + ++     K+++YGEYKC GPG+    R  Y + +   E   FL++ YI+G+ WL
Sbjct: 304 SGWRENLNRGYD-KTVFYGEYKCFGPGSHLEKRVPYTQDIDKNEVTPFLTLGYIKGSTWL 359

Query: 376 LPPP 378
           LPPP
Sbjct: 364 LPPP 359

BLAST of Tan0006809 vs. ExPASy Swiss-Prot
Match: Q9LY19 (Probable pectinesterase 48 OS=Arabidopsis thaliana OX=3702 GN=PME48 PE=2 SV=2)

HSP 1 Score: 345.9 bits (886), Expect = 6.0e-94
Identity = 169/365 (46.30%), Postives = 239/365 (65.48%), Query Frame = 0

Query: 16  IALAVVQLIIIIPSAVVEARAKPIPGEPSKLDEWIANNMREYNDRKANETGVKALDLRLA 75
           +++ +  L+I +   V      PIP    ++ +W   ++     R       K LD  L 
Sbjct: 6   VSILLGMLVIFVSPMVFADDLTPIPEGKPQVVQWFNTHVGPLAQR-------KGLDPALV 65

Query: 76  EAEDGVRLLTVRKDGRGNFTTITEAIESIPVGNRRRVVVWIGGGVYREKITIKASKPFVT 135
            AE   R++ V   G G F T+T+AI+S+P GN +RV++ +  G YREK+TI  +KPF+T
Sbjct: 66  AAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAHGEYREKVTIDRNKPFIT 125

Query: 136 LYGQKGKRPMIMYDGTASEFGTVDSATVAVESDYFVAVNVAFVNSAPMPDGRGTGGQAVA 195
           L GQ    P+I YDGTA+++GTVDSA++ + SDYF+AVN+   N+AP PDG+  G QA++
Sbjct: 126 LMGQPNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQALS 185

Query: 196 MRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIFGNGKSLYLKTSIKSVA 255
           MRISG+ AAF+ C F GFQDT+CDD G HFFKDCY++GT DFIFG+G S+YL T +  V 
Sbjct: 186 MRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVG 245

Query: 256 EGTGVITAQAREEARDESGFTFAYCNITGT-GNTYLGRAWKERTRVVFAYTFMGNLINTQ 315
           +G  VI A A + A ++SG++F +C +TGT G  YLGRAW    +VV+AYT M +++N  
Sbjct: 246 DGIRVIAAHAGKSAEEKSGYSFVHCKVTGTGGGIYLGRAWMSHPKVVYAYTEMTSVVNPT 305

Query: 316 GWSDKMHGTQPRKSMYYGEYKCMGPGASPSGRAHYARILSDQEAKAFLSMTYIQGNKWLL 375
           GW +        K+++YGEYKC GPG+  + R  + + + D+EA  FLS+ YIQG+KWLL
Sbjct: 306 GWQENKTPAHD-KTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANCFLSLGYIQGSKWLL 361

Query: 376 PPPDL 380
           PPP L
Sbjct: 366 PPPAL 361

BLAST of Tan0006809 vs. ExPASy Swiss-Prot
Match: Q84WM7 (Pectinesterase PPME1 OS=Arabidopsis thaliana OX=3702 GN=PPME1 PE=1 SV=1)

HSP 1 Score: 344.4 bits (882), Expect = 1.7e-93
Identity = 168/365 (46.03%), Postives = 239/365 (65.48%), Query Frame = 0

Query: 16  IALAVVQLIIIIPSAVVEARAKPIPGEPSKLDEWIANNMREYNDRKANETGVKALDLRLA 75
           +++ +  L++ +   V      PIP    ++ +W   N+     R       K LD  L 
Sbjct: 6   VSILLGLLMVFVTPMVFADDVTPIPEGKPQVAQWFNANVGPLAQR-------KGLDPALV 65

Query: 76  EAEDGVRLLTVRKDGRGNFTTITEAIESIPVGNRRRVVVWIGGGVYREKITIKASKPFVT 135
            AE   R++ V   G G F T+T+AI+S+P GN +RV++ +  G Y+EK+TI  +KPF+T
Sbjct: 66  AAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAPGEYKEKVTIDRNKPFIT 125

Query: 136 LYGQKGKRPMIMYDGTASEFGTVDSATVAVESDYFVAVNVAFVNSAPMPDGRGTGGQAVA 195
           L GQ    P+I YDGTA+++GTVDSA++ + SDYF+AVN+   N+AP PDG+  G QA++
Sbjct: 126 LMGQPNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQALS 185

Query: 196 MRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIFGNGKSLYLKTSIKSVA 255
           MRISG+ AAF+ C F GFQDT+CDD G HFFKDCY++GT DFIFG+G S+YL T +  V 
Sbjct: 186 MRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVG 245

Query: 256 EGTGVITAQAREEARDESGFTFAYCNITGT-GNTYLGRAWKERTRVVFAYTFMGNLINTQ 315
           +G  VI A A + A ++SG++F +C +TGT G  YLGRAW    +VV+AYT M +++N  
Sbjct: 246 DGIRVIAAHAGKSAEEKSGYSFVHCKVTGTGGGIYLGRAWMSHPKVVYAYTEMTSVVNPT 305

Query: 316 GWSDKMHGTQPRKSMYYGEYKCMGPGASPSGRAHYARILSDQEAKAFLSMTYIQGNKWLL 375
           GW +        K+++YGEYKC GPG+  + R  + + + D+EA  FLS+ YIQG+KWLL
Sbjct: 306 GWQENKTPAHD-KTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANRFLSLGYIQGSKWLL 361

Query: 376 PPPDL 380
           PPP L
Sbjct: 366 PPPAL 361

BLAST of Tan0006809 vs. ExPASy Swiss-Prot
Match: Q9LY18 (Probable pectinesterase 49 OS=Arabidopsis thaliana OX=3702 GN=PME49 PE=2 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 2.3e-90
Identity = 162/364 (44.51%), Postives = 242/364 (66.48%), Query Frame = 0

Query: 16  IALAVVQLIIIIPSAVVEA-RAKPIPGEPSKLDEWIANNMREYNDRKANETGVKALDLRL 75
           I+LA+V L++   S VV A    PIP + +++ +W   N++ ++ R+        LD  L
Sbjct: 4   ISLALVALLVFFASPVVLADDITPIPADRAQIPQWFMANVKPFSQRRG------TLDPEL 63

Query: 76  AEAEDGVRLLTVRKDGRGNFTTITEAIESIPVGNRRRVVVWIGGGVYREKITIKASKPFV 135
             AE   R++ V ++G G+F TI  AI+SIP+ N+ RV++ +  G+Y EK+T+   +P+V
Sbjct: 64  EAAEASRRVIIVNQNGGGDFKTINAAIKSIPLANKNRVIIKLAPGIYHEKVTVDVGRPYV 123

Query: 136 TLYGQKGKRPMIMYDGTASEFGTVDSATVAVESDYFVAVNVAFVNSAPMPDGRGTGGQAV 195
           TL G+ G    + Y GTA+++GTV+SAT+ V +  F+A N+  +N++PMP   GT GQA+
Sbjct: 124 TLLGKPGAETNLTYAGTAAKYGTVESATLIVWATNFLAANLNIINTSPMPK-PGTQGQAL 183

Query: 196 AMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIFGNGKSLYLKTSIKSV 255
           AMRI+GDKAAF+ C F GFQDTLCDDRG HFFK+CYI+GT DFIFG G SLYL T + +V
Sbjct: 184 AMRINGDKAAFYNCRFYGFQDTLCDDRGNHFFKNCYIEGTYDFIFGRGASLYLTTQLHAV 243

Query: 256 AEGTGVITAQAREEARDESGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTFMGNLINT 315
            +G  VI A  R+   +++G++F +C +TG G   YLGRAW    +VV++YT M +++N 
Sbjct: 244 GDGLRVIAAHNRQSTTEQNGYSFVHCKVTGVGTGIYLGRAWMSHPKVVYSYTEMSSVVNP 303

Query: 316 QGWSDKMHGTQPRKSMYYGEYKCMGPGASPSGRAHYARILSDQEAKAFLSMTYIQGNKWL 375
            GW +        K+++YGEY C GPG+  + R  + + + ++EA  FL++ YI+G+KWL
Sbjct: 304 SGWQENRVRAHD-KTVFYGEYMCTGPGSHKAKRVAHTQDIDNKEASQFLTLGYIKGSKWL 359

Query: 376 LPPP 378
           LPPP
Sbjct: 364 LPPP 359

BLAST of Tan0006809 vs. NCBI nr
Match: XP_038880021.1 (putative pectinesterase 63 [Benincasa hispida])

HSP 1 Score: 681.4 bits (1757), Expect = 4.6e-192
Identity = 337/391 (86.19%), Postives = 363/391 (92.84%), Query Frame = 0

Query: 1   MSRPTVASSFPATVAIALAVVQLII-IIPSAVVEARAKPIPGEPSKLDEWIANNMREYND 60
           MSRPTVASS PA VA++LA+ QLII II SAVVEAR K IP + SKLD+WIA+NM+EY +
Sbjct: 1   MSRPTVASSSPAAVAVSLALFQLIILIISSAVVEARTKMIPADASKLDQWIAHNMKEYEE 60

Query: 61  RKANETGVKALDLRLAEAEDGVRLLTVRKDGRGNFTTITEAIESIPVGNRRRVVVWIGGG 120
           RKANETG+KALD RLA+AED VRL+TVRKDGRGNFTTIT+AIESIP GNRRRVVVWIGGG
Sbjct: 61  RKANETGIKALDRRLAKAEDHVRLITVRKDGRGNFTTITKAIESIPSGNRRRVVVWIGGG 120

Query: 121 VYREKITIKASKPFVTLYGQKGKRPMIMYDGTASEFGTVDSATVAVESDYFVAVNVAFVN 180
           VYREKITI+ASKPFVTLYGQKG+RP+I +DGTASEFGTV SATVAVESDYFVAVN+AFVN
Sbjct: 121 VYREKITIEASKPFVTLYGQKGRRPVITFDGTASEFGTVKSATVAVESDYFVAVNLAFVN 180

Query: 181 SAPMPDGRGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIF 240
           SAPMP+  GTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIF
Sbjct: 181 SAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIF 240

Query: 241 GNGKSLYLKTSIKSVAEGTGVITAQAREEARDESGFTFAYCNITGTGNTYLGRAWKERTR 300
           GNGKSLYLKT I SVAEGTGVITAQAREEA D+SGFTFAYCNITGTGNTYLGRAWKERTR
Sbjct: 241 GNGKSLYLKTIINSVAEGTGVITAQAREEATDDSGFTFAYCNITGTGNTYLGRAWKERTR 300

Query: 301 VVFAYTFMGNLINTQGWSDKMHGTQPRKSMYYGEYKCMGPGASPSGRAHYARILSDQEAK 360
           VVFAYT+MG LINT+GWSDKMHG+QPRKSMYYGEYKCMGPGA+PSGR  YARILSD+EAK
Sbjct: 301 VVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCMGPGATPSGRVKYARILSDEEAK 360

Query: 361 AFLSMTYIQGNKWLLPPPDLSLSRSQPNSFH 391
           AFLSMTYI GNKWLLPPP+LSL  S+ N FH
Sbjct: 361 AFLSMTYIHGNKWLLPPPNLSLFHSRSNVFH 391

BLAST of Tan0006809 vs. NCBI nr
Match: XP_008450966.1 (PREDICTED: putative pectinesterase 63 [Cucumis melo])

HSP 1 Score: 675.2 bits (1741), Expect = 3.3e-190
Identity = 340/394 (86.29%), Postives = 360/394 (91.37%), Query Frame = 0

Query: 1   MSRPTVASSFPATVAIALAVV-QLIII--IPSAV-VEARAKPIPGEPSKLDEWIANNMRE 60
           M RPTVASSF ATVA+ALAV+ QLII+  IPS V VEAR K IP + SKLDEWIA+NMRE
Sbjct: 1   MFRPTVASSFSATVALALAVMFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMRE 60

Query: 61  YNDRKANETGVKALDLRLAEAEDGVRLLTVRKDGRGNFTTITEAIESIPVGNRRRVVVWI 120
           YNDRK NETG+KALD RLAEAED VRL+TVRKDGRGNF+TITEAIESIP GNRRRVVVWI
Sbjct: 61  YNDRKMNETGIKALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWI 120

Query: 121 GGGVYREKITIKASKPFVTLYGQKGKRPMIMYDGTASEFGTVDSATVAVESDYFVAVNVA 180
           GGGVYREKITI ASKPFVTLYGQKGKRP+I +DGTASEFGTV SATVAVESDYFVAVN+ 
Sbjct: 121 GGGVYREKITIDASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLT 180

Query: 181 FVNSAPMPDGRGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVD 240
           FVNSAPMP+  GTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFF+DCYIQGTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVD 240

Query: 241 FIFGNGKSLYLKTSIKSVAEGTGVITAQAREEARDESGFTFAYCNITGTGNTYLGRAWKE 300
           FIFGNGKSLYLKT+I SVAEG GVITAQARE+A DESGFTFAYCNITGTGNTYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGTGNTYLGRAWKE 300

Query: 301 RTRVVFAYTFMGNLINTQGWSDKMHGTQPRKSMYYGEYKCMGPGASPSGRAHYARILSDQ 360
           RTRVVFAYT+MG LINT+GWSDKMHG+QPRKSMYYGEYKC GPGA+ SGR  YARILSD 
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDV 360

Query: 361 EAKAFLSMTYIQGNKWLLPPPDLSLSRSQPNSFH 391
           EAKAFLSMTYI GNKWLLPPPDLS S S+PN FH
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPDLSFSPSKPNIFH 394

BLAST of Tan0006809 vs. NCBI nr
Match: XP_004144054.1 (putative pectinesterase 63 [Cucumis sativus] >KGN66331.1 hypothetical protein Csa_007174 [Cucumis sativus])

HSP 1 Score: 673.7 bits (1737), Expect = 9.5e-190
Identity = 334/394 (84.77%), Postives = 358/394 (90.86%), Query Frame = 0

Query: 1   MSRPTVASSFPATVAIALAVVQ---LIIIIPS-AVVEARAKPIPGEPSKLDEWIANNMRE 60
           M RPTVASSFPATVA A+ V+    ++IIIPS  VVEA  K IP + SKLDEWI +NM+E
Sbjct: 1   MFRPTVASSFPATVAFAMGVMLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKE 60

Query: 61  YNDRKANETGVKALDLRLAEAEDGVRLLTVRKDGRGNFTTITEAIESIPVGNRRRVVVWI 120
           YNDRK NETG+KALD RLAEAED V+L+TVRKDGRGNF+TITEAI+SIP GNRRRVVVWI
Sbjct: 61  YNDRKTNETGIKALDRRLAEAEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWI 120

Query: 121 GGGVYREKITIKASKPFVTLYGQKGKRPMIMYDGTASEFGTVDSATVAVESDYFVAVNVA 180
           GGGVYREKITI ASKPFVTLYGQKGKRPMI +DGTASEFGTV SATVAVESDYFVAVN+ 
Sbjct: 121 GGGVYREKITIDASKPFVTLYGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLT 180

Query: 181 FVNSAPMPDGRGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVD 240
           FVNSAPMP+  GTGGQAVAMRISGDKAAFH CHFIGFQDTLCDDRGRHFFKDCY+QGTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVD 240

Query: 241 FIFGNGKSLYLKTSIKSVAEGTGVITAQAREEARDESGFTFAYCNITGTGNTYLGRAWKE 300
           FIFGNGKSLYLKT+I SVAEGTGVITAQARE+A DESGFTFAYCNITGTG+TYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTTINSVAEGTGVITAQAREDATDESGFTFAYCNITGTGDTYLGRAWKE 300

Query: 301 RTRVVFAYTFMGNLINTQGWSDKMHGTQPRKSMYYGEYKCMGPGASPSGRAHYARILSDQ 360
           RTRVVFAYT+MG LINT+GWSDKMHG+QPRKSMYYGEYKC GPGA+PSGR  YARILSD 
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDV 360

Query: 361 EAKAFLSMTYIQGNKWLLPPPDLSLSRSQPNSFH 391
           EAKAFLSMTYI GNKWLLPPPDLSLS S+PN FH
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPDLSLSPSKPNIFH 394

BLAST of Tan0006809 vs. NCBI nr
Match: XP_022147590.1 (putative pectinesterase 63 [Momordica charantia])

HSP 1 Score: 662.1 bits (1707), Expect = 2.9e-186
Identity = 325/390 (83.33%), Postives = 350/390 (89.74%), Query Frame = 0

Query: 1   MSRPTVASSFPATVAIALAVVQLIIIIPSAVVEARAKPIPGEPSKLDEWIANNMREYNDR 60
           M RP VASS P  VAIALAV+QLIII+P+AVV AR KPIP EPSKLDEWI NNM+EY++R
Sbjct: 1   MYRPAVASSSPVAVAIALAVIQLIIILPAAVV-ARTKPIPAEPSKLDEWIENNMKEYHER 60

Query: 61  KANETGVKALDLRLAEAEDGVRLLTVRKDGRGNFTTITEAIESIPVGNRRRVVVWIGGGV 120
           KAN+TGVKALD RLA AED VRL+TVR DGRGNFTTIT AIESIP GN  RV+VWI GG+
Sbjct: 61  KANDTGVKALDKRLAMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWIAGGI 120

Query: 121 YREKITIKASKPFVTLYGQKGKRPMIMYDGTASEFGTVDSATVAVESDYFVAVNVAFVNS 180
           YREKITI ASKPFVTLYGQ GK P+I +DGTASEFGTV SATVAVESDYFVAVN+AFVNS
Sbjct: 121 YREKITIDASKPFVTLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIAFVNS 180

Query: 181 APMPDGRGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIFG 240
           A MPD R +GGQAVAMRISGDKAAFH CHFIGFQDTLCDDRGRHFFKDCYI+GTVDFIFG
Sbjct: 181 AAMPDVRVSGGQAVAMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFG 240

Query: 241 NGKSLYLKTSIKSVAEGTGVITAQAREEARDESGFTFAYCNITGTGNTYLGRAWKERTRV 300
           NGKSLYLKT I SVAEG GVITAQARE+A D+SGFTF YCNITGTGNTYLGRAWKERTRV
Sbjct: 241 NGKSLYLKTRINSVAEGMGVITAQAREDATDDSGFTFVYCNITGTGNTYLGRAWKERTRV 300

Query: 301 VFAYTFMGNLINTQGWSDKMHGTQPRKSMYYGEYKCMGPGASPSGRAHYARILSDQEAKA 360
           VFAYT+MGNLINT+GWSDKMH  +PRKSMYYGEYKCMGPGA+P+GR  YARILSD+EA+A
Sbjct: 301 VFAYTYMGNLINTEGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDEEARA 360

Query: 361 FLSMTYIQGNKWLLPPPDLSLSRSQPNSFH 391
           FLSMTYI GNKWLLPPP LS SRS PN+FH
Sbjct: 361 FLSMTYIHGNKWLLPPPLLSFSRSHPNTFH 389

BLAST of Tan0006809 vs. NCBI nr
Match: XP_022987214.1 (putative pectinesterase 63 [Cucurbita maxima])

HSP 1 Score: 646.4 bits (1666), Expect = 1.6e-181
Identity = 321/383 (83.81%), Postives = 344/383 (89.82%), Query Frame = 0

Query: 1   MSRPTVASSFPATVAIALAVVQLI-IIIPSA-VVEARAKPIPGEPSKLDEWIANNMREYN 60
           MSRP VASSFPATVA+ LAVVQLI ++IP+A VVEAR KPIP EPS+LDEWIA+NMREY 
Sbjct: 1   MSRPAVASSFPATVALVLAVVQLITMLIPAAIVVEARTKPIPAEPSQLDEWIAHNMREYR 60

Query: 61  DRKANETGVKALDLRLAEAEDGVRLLTVRKDGRGNFTTITEAIESIPVGNRRRVVVWIGG 120
            RKA ETG KALD RLA+AED  RL+TV++DG+GNF+TIT+AIESIP GN  RVVVWIGG
Sbjct: 61  QRKAKETGAKALDRRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWIGG 120

Query: 121 GVYREKITIKASKPFVTLYGQKGKRPMIMYDGTASEFGTVDSATVAVESDYFVAVNVAFV 180
           G YREKITI +SKPFVTLYGQ GK P+I YDGTASEFGTV+SATVAVESDYFVAVNVAFV
Sbjct: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVAFV 180

Query: 181 NSAPMPDGRGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFI 240
           NSAPMPD   TGGQAVAMRISGDKAAFH C FIGFQDTLCDDRGRHFFKDCYI+GTVDFI
Sbjct: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240

Query: 241 FGNGKSLYLKTSIKSVAEGTGVITAQAREEARDESGFTFAYCNITGTGNTYLGRAWKERT 300
           FGNGKSLYLKT I SVAEGTGVITAQARE + DESGFTFAYCNITGTGNTYLGRAWKERT
Sbjct: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDESGFTFAYCNITGTGNTYLGRAWKERT 300

Query: 301 RVVFAYTFMGNLINTQGWSDKMHGTQPRKSMYYGEYKCMGPGASPSGRAHYARILSDQEA 360
           RVVFAYT+MG LINTQGWSDKM GT+PRKSMYYGEYKC GPGA  +GRAHY RIL+D EA
Sbjct: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA 360

Query: 361 KAFLSMTYIQGNKWLLPPPDLSL 382
           KAFLSMTYI GNKW+LPPP LSL
Sbjct: 361 KAFLSMTYIHGNKWVLPPPRLSL 383

BLAST of Tan0006809 vs. ExPASy TrEMBL
Match: A0A1S3BRI5 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492397 PE=4 SV=1)

HSP 1 Score: 675.2 bits (1741), Expect = 1.6e-190
Identity = 340/394 (86.29%), Postives = 360/394 (91.37%), Query Frame = 0

Query: 1   MSRPTVASSFPATVAIALAVV-QLIII--IPSAV-VEARAKPIPGEPSKLDEWIANNMRE 60
           M RPTVASSF ATVA+ALAV+ QLII+  IPS V VEAR K IP + SKLDEWIA+NMRE
Sbjct: 1   MFRPTVASSFSATVALALAVMFQLIILITIPSTVLVEARTKTIPADSSKLDEWIAHNMRE 60

Query: 61  YNDRKANETGVKALDLRLAEAEDGVRLLTVRKDGRGNFTTITEAIESIPVGNRRRVVVWI 120
           YNDRK NETG+KALD RLAEAED VRL+TVRKDGRGNF+TITEAIESIP GNRRRVVVWI
Sbjct: 61  YNDRKMNETGIKALDRRLAEAEDCVRLITVRKDGRGNFSTITEAIESIPSGNRRRVVVWI 120

Query: 121 GGGVYREKITIKASKPFVTLYGQKGKRPMIMYDGTASEFGTVDSATVAVESDYFVAVNVA 180
           GGGVYREKITI ASKPFVTLYGQKGKRP+I +DGTASEFGTV SATVAVESDYFVAVN+ 
Sbjct: 121 GGGVYREKITIDASKPFVTLYGQKGKRPVITFDGTASEFGTVKSATVAVESDYFVAVNLT 180

Query: 181 FVNSAPMPDGRGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVD 240
           FVNSAPMP+  GTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFF+DCYIQGTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFRDCYIQGTVD 240

Query: 241 FIFGNGKSLYLKTSIKSVAEGTGVITAQAREEARDESGFTFAYCNITGTGNTYLGRAWKE 300
           FIFGNGKSLYLKT+I SVAEG GVITAQARE+A DESGFTFAYCNITGTGNTYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTTINSVAEGIGVITAQAREDATDESGFTFAYCNITGTGNTYLGRAWKE 300

Query: 301 RTRVVFAYTFMGNLINTQGWSDKMHGTQPRKSMYYGEYKCMGPGASPSGRAHYARILSDQ 360
           RTRVVFAYT+MG LINT+GWSDKMHG+QPRKSMYYGEYKC GPGA+ SGR  YARILSD 
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATSSGRVKYARILSDV 360

Query: 361 EAKAFLSMTYIQGNKWLLPPPDLSLSRSQPNSFH 391
           EAKAFLSMTYI GNKWLLPPPDLS S S+PN FH
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPDLSFSPSKPNIFH 394

BLAST of Tan0006809 vs. ExPASy TrEMBL
Match: A0A0A0M299 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G597780 PE=4 SV=1)

HSP 1 Score: 673.7 bits (1737), Expect = 4.6e-190
Identity = 334/394 (84.77%), Postives = 358/394 (90.86%), Query Frame = 0

Query: 1   MSRPTVASSFPATVAIALAVVQ---LIIIIPS-AVVEARAKPIPGEPSKLDEWIANNMRE 60
           M RPTVASSFPATVA A+ V+    ++IIIPS  VVEA  K IP + SKLDEWI +NM+E
Sbjct: 1   MFRPTVASSFPATVAFAMGVMLQLIILIIIPSTVVVEAHTKTIPADSSKLDEWIGHNMKE 60

Query: 61  YNDRKANETGVKALDLRLAEAEDGVRLLTVRKDGRGNFTTITEAIESIPVGNRRRVVVWI 120
           YNDRK NETG+KALD RLAEAED V+L+TVRKDGRGNF+TITEAI+SIP GNRRRVVVWI
Sbjct: 61  YNDRKTNETGIKALDRRLAEAEDCVQLITVRKDGRGNFSTITEAIDSIPSGNRRRVVVWI 120

Query: 121 GGGVYREKITIKASKPFVTLYGQKGKRPMIMYDGTASEFGTVDSATVAVESDYFVAVNVA 180
           GGGVYREKITI ASKPFVTLYGQKGKRPMI +DGTASEFGTV SATVAVESDYFVAVN+ 
Sbjct: 121 GGGVYREKITIDASKPFVTLYGQKGKRPMITFDGTASEFGTVKSATVAVESDYFVAVNLT 180

Query: 181 FVNSAPMPDGRGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVD 240
           FVNSAPMP+  GTGGQAVAMRISGDKAAFH CHFIGFQDTLCDDRGRHFFKDCY+QGTVD
Sbjct: 181 FVNSAPMPELGGTGGQAVAMRISGDKAAFHGCHFIGFQDTLCDDRGRHFFKDCYVQGTVD 240

Query: 241 FIFGNGKSLYLKTSIKSVAEGTGVITAQAREEARDESGFTFAYCNITGTGNTYLGRAWKE 300
           FIFGNGKSLYLKT+I SVAEGTGVITAQARE+A DESGFTFAYCNITGTG+TYLGRAWKE
Sbjct: 241 FIFGNGKSLYLKTTINSVAEGTGVITAQAREDATDESGFTFAYCNITGTGDTYLGRAWKE 300

Query: 301 RTRVVFAYTFMGNLINTQGWSDKMHGTQPRKSMYYGEYKCMGPGASPSGRAHYARILSDQ 360
           RTRVVFAYT+MG LINT+GWSDKMHG+QPRKSMYYGEYKC GPGA+PSGR  YARILSD 
Sbjct: 301 RTRVVFAYTYMGTLINTEGWSDKMHGSQPRKSMYYGEYKCKGPGATPSGRVKYARILSDV 360

Query: 361 EAKAFLSMTYIQGNKWLLPPPDLSLSRSQPNSFH 391
           EAKAFLSMTYI GNKWLLPPPDLSLS S+PN FH
Sbjct: 361 EAKAFLSMTYIHGNKWLLPPPDLSLSPSKPNIFH 394

BLAST of Tan0006809 vs. ExPASy TrEMBL
Match: A0A6J1D0K0 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016481 PE=4 SV=1)

HSP 1 Score: 662.1 bits (1707), Expect = 1.4e-186
Identity = 325/390 (83.33%), Postives = 350/390 (89.74%), Query Frame = 0

Query: 1   MSRPTVASSFPATVAIALAVVQLIIIIPSAVVEARAKPIPGEPSKLDEWIANNMREYNDR 60
           M RP VASS P  VAIALAV+QLIII+P+AVV AR KPIP EPSKLDEWI NNM+EY++R
Sbjct: 1   MYRPAVASSSPVAVAIALAVIQLIIILPAAVV-ARTKPIPAEPSKLDEWIENNMKEYHER 60

Query: 61  KANETGVKALDLRLAEAEDGVRLLTVRKDGRGNFTTITEAIESIPVGNRRRVVVWIGGGV 120
           KAN+TGVKALD RLA AED VRL+TVR DGRGNFTTIT AIESIP GN  RV+VWI GG+
Sbjct: 61  KANDTGVKALDKRLAMAEDAVRLITVRPDGRGNFTTITAAIESIPAGNTARVIVWIAGGI 120

Query: 121 YREKITIKASKPFVTLYGQKGKRPMIMYDGTASEFGTVDSATVAVESDYFVAVNVAFVNS 180
           YREKITI ASKPFVTLYGQ GK P+I +DGTASEFGTV SATVAVESDYFVAVN+AFVNS
Sbjct: 121 YREKITIDASKPFVTLYGQNGKMPVITFDGTASEFGTVQSATVAVESDYFVAVNIAFVNS 180

Query: 181 APMPDGRGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIFG 240
           A MPD R +GGQAVAMRISGDKAAFH CHFIGFQDTLCDDRGRHFFKDCYI+GTVDFIFG
Sbjct: 181 AAMPDVRVSGGQAVAMRISGDKAAFHHCHFIGFQDTLCDDRGRHFFKDCYIEGTVDFIFG 240

Query: 241 NGKSLYLKTSIKSVAEGTGVITAQAREEARDESGFTFAYCNITGTGNTYLGRAWKERTRV 300
           NGKSLYLKT I SVAEG GVITAQARE+A D+SGFTF YCNITGTGNTYLGRAWKERTRV
Sbjct: 241 NGKSLYLKTRINSVAEGMGVITAQAREDATDDSGFTFVYCNITGTGNTYLGRAWKERTRV 300

Query: 301 VFAYTFMGNLINTQGWSDKMHGTQPRKSMYYGEYKCMGPGASPSGRAHYARILSDQEAKA 360
           VFAYT+MGNLINT+GWSDKMH  +PRKSMYYGEYKCMGPGA+P+GR  YARILSD+EA+A
Sbjct: 301 VFAYTYMGNLINTEGWSDKMHHGEPRKSMYYGEYKCMGPGATPAGRVKYARILSDEEARA 360

Query: 361 FLSMTYIQGNKWLLPPPDLSLSRSQPNSFH 391
           FLSMTYI GNKWLLPPP LS SRS PN+FH
Sbjct: 361 FLSMTYIHGNKWLLPPPLLSFSRSHPNTFH 389

BLAST of Tan0006809 vs. ExPASy TrEMBL
Match: A0A6J1JI92 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111484834 PE=4 SV=1)

HSP 1 Score: 646.4 bits (1666), Expect = 7.9e-182
Identity = 321/383 (83.81%), Postives = 344/383 (89.82%), Query Frame = 0

Query: 1   MSRPTVASSFPATVAIALAVVQLI-IIIPSA-VVEARAKPIPGEPSKLDEWIANNMREYN 60
           MSRP VASSFPATVA+ LAVVQLI ++IP+A VVEAR KPIP EPS+LDEWIA+NMREY 
Sbjct: 1   MSRPAVASSFPATVALVLAVVQLITMLIPAAIVVEARTKPIPAEPSQLDEWIAHNMREYR 60

Query: 61  DRKANETGVKALDLRLAEAEDGVRLLTVRKDGRGNFTTITEAIESIPVGNRRRVVVWIGG 120
            RKA ETG KALD RLA+AED  RL+TV++DG+GNF+TIT+AIESIP GN  RVVVWIGG
Sbjct: 61  QRKAKETGAKALDRRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNSERVVVWIGG 120

Query: 121 GVYREKITIKASKPFVTLYGQKGKRPMIMYDGTASEFGTVDSATVAVESDYFVAVNVAFV 180
           G YREKITI +SKPFVTLYGQ GK P+I YDGTASEFGTV+SATVAVESDYFVAVNVAFV
Sbjct: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTVESATVAVESDYFVAVNVAFV 180

Query: 181 NSAPMPDGRGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFI 240
           NSAPMPD   TGGQAVAMRISGDKAAFH C FIGFQDTLCDDRGRHFFKDCYI+GTVDFI
Sbjct: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240

Query: 241 FGNGKSLYLKTSIKSVAEGTGVITAQAREEARDESGFTFAYCNITGTGNTYLGRAWKERT 300
           FGNGKSLYLKT I SVAEGTGVITAQARE + DESGFTFAYCNITGTGNTYLGRAWKERT
Sbjct: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDESGFTFAYCNITGTGNTYLGRAWKERT 300

Query: 301 RVVFAYTFMGNLINTQGWSDKMHGTQPRKSMYYGEYKCMGPGASPSGRAHYARILSDQEA 360
           RVVFAYT+MG LINTQGWSDKM GT+PRKSMYYGEYKC GPGA  +GRAHY RIL+D EA
Sbjct: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA 360

Query: 361 KAFLSMTYIQGNKWLLPPPDLSL 382
           KAFLSMTYI GNKW+LPPP LSL
Sbjct: 361 KAFLSMTYIHGNKWVLPPPRLSL 383

BLAST of Tan0006809 vs. ExPASy TrEMBL
Match: A0A6J1HB45 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111461773 PE=4 SV=1)

HSP 1 Score: 644.4 bits (1661), Expect = 3.0e-181
Identity = 320/383 (83.55%), Postives = 343/383 (89.56%), Query Frame = 0

Query: 1   MSRPTVASSFPATVAIALAVVQLI-IIIPSA-VVEARAKPIPGEPSKLDEWIANNMREYN 60
           MSRP VASSFPATVA+ LAVVQLI I+IP+A VVEAR K IP EPS+LDEWIA+NMREY 
Sbjct: 1   MSRPAVASSFPATVALVLAVVQLITILIPAAIVVEARTKTIPAEPSQLDEWIAHNMREYR 60

Query: 61  DRKANETGVKALDLRLAEAEDGVRLLTVRKDGRGNFTTITEAIESIPVGNRRRVVVWIGG 120
            RKANETG K LD RLA+AED  RL+TV++DG+GNF+TIT+AIESIP GNR RVVVWIGG
Sbjct: 61  QRKANETGAKTLDPRLAKAEDCARLITVKQDGQGNFSTITQAIESIPSGNRERVVVWIGG 120

Query: 121 GVYREKITIKASKPFVTLYGQKGKRPMIMYDGTASEFGTVDSATVAVESDYFVAVNVAFV 180
           G YREKITI +SKPFVTLYGQ GK P+I YDGTASEFGT +SATVAVESDYFVAVNVAFV
Sbjct: 121 GTYREKITIDSSKPFVTLYGQTGKMPVISYDGTASEFGTAESATVAVESDYFVAVNVAFV 180

Query: 181 NSAPMPDGRGTGGQAVAMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFI 240
           NSAPMPD   TGGQAVAMRISGDKAAFH C FIGFQDTLCDDRGRHFFKDCYI+GTVDFI
Sbjct: 181 NSAPMPDAGRTGGQAVAMRISGDKAAFHHCQFIGFQDTLCDDRGRHFFKDCYIEGTVDFI 240

Query: 241 FGNGKSLYLKTSIKSVAEGTGVITAQAREEARDESGFTFAYCNITGTGNTYLGRAWKERT 300
           FGNGKSLYLKT I SVAEGTGVITAQARE + D+SGFTFAYCNITGTGNTYLGRAWKERT
Sbjct: 241 FGNGKSLYLKTVINSVAEGTGVITAQARENSTDDSGFTFAYCNITGTGNTYLGRAWKERT 300

Query: 301 RVVFAYTFMGNLINTQGWSDKMHGTQPRKSMYYGEYKCMGPGASPSGRAHYARILSDQEA 360
           RVVFAYT+MG LINTQGWSDKM GT+PRKSMYYGEYKC GPGA  +GRAHY RIL+D EA
Sbjct: 301 RVVFAYTYMGTLINTQGWSDKMQGTEPRKSMYYGEYKCKGPGADSTGRAHYTRILTDDEA 360

Query: 361 KAFLSMTYIQGNKWLLPPPDLSL 382
           KAFLSMTYI GNKW+LPPP LSL
Sbjct: 361 KAFLSMTYIHGNKWVLPPPRLSL 383

BLAST of Tan0006809 vs. TAIR 10
Match: AT5G61680.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 356.7 bits (914), Expect = 2.4e-98
Identity = 175/364 (48.08%), Postives = 239/364 (65.66%), Query Frame = 0

Query: 16  IALAVVQLIIIIPSAVVEAR-AKPIPGEPSKLDEWIANNMREYNDRKANETGVKALDLRL 75
           ++L V  L+++I S VV    A PIP    ++++W   N                     
Sbjct: 6   VSLIVTILLVVITSPVVFGNDAAPIPENKGRIEQWFNTN--------------------- 65

Query: 76  AEAEDGVRLLTVRKDGRGNFTTITEAIESIPVGNRRRVVVWIGGGVYREKITIKASKPFV 135
                      V+++GRG+F TITEAI S+  GN RRV++ IG GVY+EK+TI  SKPF+
Sbjct: 66  -----------VKQNGRGHFKTITEAINSVRAGNTRRVIIKIGPGVYKEKVTIDRSKPFI 125

Query: 136 TLYGQKGKRPMIMYDGTASEFGTVDSATVAVESDYFVAVNVAFVNSAPMPDGRGTGGQAV 195
           TLYG     P++ +DGTA+++GTVDSAT+ V SDYF+AVN+   NSAPMPDG+  G QA+
Sbjct: 126 TLYGHPNAMPVLTFDGTAAQYGTVDSATLIVLSDYFMAVNIILKNSAPMPDGKRKGAQAL 185

Query: 196 AMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIFGNGKSLYLKTSIKSV 255
           +MRISG+KAAF+ C F G+QDT+CDD G HFFKDCYI+GT DFIFG+G+SLYL T +  V
Sbjct: 186 SMRISGNKAAFYNCKFYGYQDTICDDTGNHFFKDCYIEGTFDFIFGSGRSLYLGTQLNVV 245

Query: 256 AEGTGVITAQAREEARDESGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTFMGNLINT 315
            +G  VITA A + A ++SG++F +C +TGTG   YLGR+W    +VV+AYT M +++N 
Sbjct: 246 GDGIRVITAHAGKSAAEKSGYSFVHCKVTGTGTGIYLGRSWMSHPKVVYAYTDMSSVVNP 305

Query: 316 QGWSDKMHGTQPRKSMYYGEYKCMGPGASPSGRAHYARILSDQEAKAFLSMTYIQGNKWL 375
            GW +     +  K+++YGEYKC G G+    R  Y + + D EAK F+S+ YIQG+ WL
Sbjct: 306 SGWQENREAGRD-KTVFYGEYKCTGTGSHKEKRVKYTQDIDDIEAKYFISLGYIQGSSWL 336

Query: 376 LPPP 378
           LPPP
Sbjct: 366 LPPP 336

BLAST of Tan0006809 vs. TAIR 10
Match: AT5G07430.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 348.2 bits (892), Expect = 8.5e-96
Identity = 169/364 (46.43%), Postives = 244/364 (67.03%), Query Frame = 0

Query: 16  IALAVVQLIIIIPSAVVEAR-AKPIPGEPSKLDEWIANNMREYNDRKANETGVKALDLRL 75
           I+++VV  +++  S VV A    PIP   +++ +W   N++ Y+ RK        LD  L
Sbjct: 4   ISMSVVAFLVVFASPVVLATDTDPIPENRAQIPQWFKTNVKPYSQRKG------TLDPAL 63

Query: 76  AEAEDGVRLLTVRKDGRGNFTTITEAIESIPVGNRRRVVVWIGGGVYREKITIKASKPFV 135
             AE   +++TV + G  NF T+ EAI+SIP GN+ RV++ +  GVY EK+TI  ++PF+
Sbjct: 64  EAAEAARQIITVNQKGGANFKTLNEAIKSIPTGNKNRVIIKLAPGVYNEKVTIDIARPFI 123

Query: 136 TLYGQKGKRPMIMYDGTASEFGTVDSATVAVESDYFVAVNVAFVNSAPMPDGRGTGGQAV 195
           TL GQ G   ++ Y GTA+++GTV+SAT+ V ++YF A ++   N+APMP   G+ GQA+
Sbjct: 124 TLLGQPGAETVLTYHGTAAQYGTVESATLIVWAEYFQAAHLTIKNTAPMPK-PGSQGQAL 183

Query: 196 AMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIFGNGKSLYLKTSIKSV 255
           AMRI+ DKAAF+ C F GFQDTLCDD+G HFFKDCYI+GT DFIFG G SLYL T + +V
Sbjct: 184 AMRINADKAAFYSCRFHGFQDTLCDDKGNHFFKDCYIEGTYDFIFGRGASLYLNTQLHAV 243

Query: 256 AEGTGVITAQAREEARDESGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTFMGNLINT 315
            +G  VITAQ R+ A +++G+TF +C +TGTG   YLGR+W    +VV+A+T M +++N 
Sbjct: 244 GDGLRVITAQGRQSATEQNGYTFVHCKVTGTGTGIYLGRSWMSHPKVVYAFTEMTSVVNP 303

Query: 316 QGWSDKMHGTQPRKSMYYGEYKCMGPGASPSGRAHYARILSDQEAKAFLSMTYIQGNKWL 375
            GW + ++     K+++YGEYKC GPG+    R  Y + +   E   FL++ YI+G+ WL
Sbjct: 304 SGWRENLNRGYD-KTVFYGEYKCFGPGSHLEKRVPYTQDIDKNEVTPFLTLGYIKGSTWL 359

Query: 376 LPPP 378
           LPPP
Sbjct: 364 LPPP 359

BLAST of Tan0006809 vs. TAIR 10
Match: AT5G07410.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 345.9 bits (886), Expect = 4.2e-95
Identity = 169/365 (46.30%), Postives = 239/365 (65.48%), Query Frame = 0

Query: 16  IALAVVQLIIIIPSAVVEARAKPIPGEPSKLDEWIANNMREYNDRKANETGVKALDLRLA 75
           +++ +  L+I +   V      PIP    ++ +W   ++     R       K LD  L 
Sbjct: 6   VSILLGMLVIFVSPMVFADDLTPIPEGKPQVVQWFNTHVGPLAQR-------KGLDPALV 65

Query: 76  EAEDGVRLLTVRKDGRGNFTTITEAIESIPVGNRRRVVVWIGGGVYREKITIKASKPFVT 135
            AE   R++ V   G G F T+T+AI+S+P GN +RV++ +  G YREK+TI  +KPF+T
Sbjct: 66  AAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAHGEYREKVTIDRNKPFIT 125

Query: 136 LYGQKGKRPMIMYDGTASEFGTVDSATVAVESDYFVAVNVAFVNSAPMPDGRGTGGQAVA 195
           L GQ    P+I YDGTA+++GTVDSA++ + SDYF+AVN+   N+AP PDG+  G QA++
Sbjct: 126 LMGQPNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQALS 185

Query: 196 MRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIFGNGKSLYLKTSIKSVA 255
           MRISG+ AAF+ C F GFQDT+CDD G HFFKDCY++GT DFIFG+G S+YL T +  V 
Sbjct: 186 MRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVG 245

Query: 256 EGTGVITAQAREEARDESGFTFAYCNITGT-GNTYLGRAWKERTRVVFAYTFMGNLINTQ 315
           +G  VI A A + A ++SG++F +C +TGT G  YLGRAW    +VV+AYT M +++N  
Sbjct: 246 DGIRVIAAHAGKSAEEKSGYSFVHCKVTGTGGGIYLGRAWMSHPKVVYAYTEMTSVVNPT 305

Query: 316 GWSDKMHGTQPRKSMYYGEYKCMGPGASPSGRAHYARILSDQEAKAFLSMTYIQGNKWLL 375
           GW +        K+++YGEYKC GPG+  + R  + + + D+EA  FLS+ YIQG+KWLL
Sbjct: 306 GWQENKTPAHD-KTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANCFLSLGYIQGSKWLL 361

Query: 376 PPPDL 380
           PPP L
Sbjct: 366 PPPAL 361

BLAST of Tan0006809 vs. TAIR 10
Match: AT1G69940.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 344.4 bits (882), Expect = 1.2e-94
Identity = 168/365 (46.03%), Postives = 239/365 (65.48%), Query Frame = 0

Query: 16  IALAVVQLIIIIPSAVVEARAKPIPGEPSKLDEWIANNMREYNDRKANETGVKALDLRLA 75
           +++ +  L++ +   V      PIP    ++ +W   N+     R       K LD  L 
Sbjct: 6   VSILLGLLMVFVTPMVFADDVTPIPEGKPQVAQWFNANVGPLAQR-------KGLDPALV 65

Query: 76  EAEDGVRLLTVRKDGRGNFTTITEAIESIPVGNRRRVVVWIGGGVYREKITIKASKPFVT 135
            AE   R++ V   G G F T+T+AI+S+P GN +RV++ +  G Y+EK+TI  +KPF+T
Sbjct: 66  AAEAAPRIINVNPKG-GEFKTLTDAIKSVPAGNTKRVIIKMAPGEYKEKVTIDRNKPFIT 125

Query: 136 LYGQKGKRPMIMYDGTASEFGTVDSATVAVESDYFVAVNVAFVNSAPMPDGRGTGGQAVA 195
           L GQ    P+I YDGTA+++GTVDSA++ + SDYF+AVN+   N+AP PDG+  G QA++
Sbjct: 126 LMGQPNAMPVITYDGTAAKYGTVDSASLIILSDYFMAVNIVVKNTAPAPDGKTKGAQALS 185

Query: 196 MRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIFGNGKSLYLKTSIKSVA 255
           MRISG+ AAF+ C F GFQDT+CDD G HFFKDCY++GT DFIFG+G S+YL T +  V 
Sbjct: 186 MRISGNFAAFYNCKFYGFQDTICDDTGNHFFKDCYVEGTFDFIFGSGTSMYLGTQLHVVG 245

Query: 256 EGTGVITAQAREEARDESGFTFAYCNITGT-GNTYLGRAWKERTRVVFAYTFMGNLINTQ 315
           +G  VI A A + A ++SG++F +C +TGT G  YLGRAW    +VV+AYT M +++N  
Sbjct: 246 DGIRVIAAHAGKSAEEKSGYSFVHCKVTGTGGGIYLGRAWMSHPKVVYAYTEMTSVVNPT 305

Query: 316 GWSDKMHGTQPRKSMYYGEYKCMGPGASPSGRAHYARILSDQEAKAFLSMTYIQGNKWLL 375
           GW +        K+++YGEYKC GPG+  + R  + + + D+EA  FLS+ YIQG+KWLL
Sbjct: 306 GWQENKTPAHD-KTVFYGEYKCSGPGSHKAKRVPFTQDIDDKEANRFLSLGYIQGSKWLL 361

Query: 376 PPPDL 380
           PPP L
Sbjct: 366 PPPAL 361

BLAST of Tan0006809 vs. TAIR 10
Match: AT5G07420.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 334.0 bits (855), Expect = 1.7e-91
Identity = 162/364 (44.51%), Postives = 242/364 (66.48%), Query Frame = 0

Query: 16  IALAVVQLIIIIPSAVVEA-RAKPIPGEPSKLDEWIANNMREYNDRKANETGVKALDLRL 75
           I+LA+V L++   S VV A    PIP + +++ +W   N++ ++ R+        LD  L
Sbjct: 4   ISLALVALLVFFASPVVLADDITPIPADRAQIPQWFMANVKPFSQRRG------TLDPEL 63

Query: 76  AEAEDGVRLLTVRKDGRGNFTTITEAIESIPVGNRRRVVVWIGGGVYREKITIKASKPFV 135
             AE   R++ V ++G G+F TI  AI+SIP+ N+ RV++ +  G+Y EK+T+   +P+V
Sbjct: 64  EAAEASRRVIIVNQNGGGDFKTINAAIKSIPLANKNRVIIKLAPGIYHEKVTVDVGRPYV 123

Query: 136 TLYGQKGKRPMIMYDGTASEFGTVDSATVAVESDYFVAVNVAFVNSAPMPDGRGTGGQAV 195
           TL G+ G    + Y GTA+++GTV+SAT+ V +  F+A N+  +N++PMP   GT GQA+
Sbjct: 124 TLLGKPGAETNLTYAGTAAKYGTVESATLIVWATNFLAANLNIINTSPMPK-PGTQGQAL 183

Query: 196 AMRISGDKAAFHECHFIGFQDTLCDDRGRHFFKDCYIQGTVDFIFGNGKSLYLKTSIKSV 255
           AMRI+GDKAAF+ C F GFQDTLCDDRG HFFK+CYI+GT DFIFG G SLYL T + +V
Sbjct: 184 AMRINGDKAAFYNCRFYGFQDTLCDDRGNHFFKNCYIEGTYDFIFGRGASLYLTTQLHAV 243

Query: 256 AEGTGVITAQAREEARDESGFTFAYCNITGTG-NTYLGRAWKERTRVVFAYTFMGNLINT 315
            +G  VI A  R+   +++G++F +C +TG G   YLGRAW    +VV++YT M +++N 
Sbjct: 244 GDGLRVIAAHNRQSTTEQNGYSFVHCKVTGVGTGIYLGRAWMSHPKVVYSYTEMSSVVNP 303

Query: 316 QGWSDKMHGTQPRKSMYYGEYKCMGPGASPSGRAHYARILSDQEAKAFLSMTYIQGNKWL 375
            GW +        K+++YGEY C GPG+  + R  + + + ++EA  FL++ YI+G+KWL
Sbjct: 304 SGWQENRVRAHD-KTVFYGEYMCTGPGSHKAKRVAHTQDIDNKEASQFLTLGYIKGSKWL 359

Query: 376 LPPP 378
           LPPP
Sbjct: 364 LPPP 359

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FKF33.4e-9748.08Putative pectinesterase 63 OS=Arabidopsis thaliana OX=3702 GN=PME63 PE=3 SV=2[more]
Q9LY171.2e-9446.43Probable pectinesterase 50 OS=Arabidopsis thaliana OX=3702 GN=PME50 PE=2 SV=1[more]
Q9LY196.0e-9446.30Probable pectinesterase 48 OS=Arabidopsis thaliana OX=3702 GN=PME48 PE=2 SV=2[more]
Q84WM71.7e-9346.03Pectinesterase PPME1 OS=Arabidopsis thaliana OX=3702 GN=PPME1 PE=1 SV=1[more]
Q9LY182.3e-9044.51Probable pectinesterase 49 OS=Arabidopsis thaliana OX=3702 GN=PME49 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038880021.14.6e-19286.19putative pectinesterase 63 [Benincasa hispida][more]
XP_008450966.13.3e-19086.29PREDICTED: putative pectinesterase 63 [Cucumis melo][more]
XP_004144054.19.5e-19084.77putative pectinesterase 63 [Cucumis sativus] >KGN66331.1 hypothetical protein Cs... [more]
XP_022147590.12.9e-18683.33putative pectinesterase 63 [Momordica charantia][more]
XP_022987214.11.6e-18183.81putative pectinesterase 63 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A1S3BRI51.6e-19086.29Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492397 PE=4 SV=1[more]
A0A0A0M2994.6e-19084.77Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G597780 PE=4 SV=1[more]
A0A6J1D0K01.4e-18683.33Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111016481 PE=4 SV=1[more]
A0A6J1JI927.9e-18283.81Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111484834 PE=4 SV=1[more]
A0A6J1HB453.0e-18183.55Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111461773 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G61680.12.4e-9848.08Pectin lyase-like superfamily protein [more]
AT5G07430.18.5e-9646.43Pectin lyase-like superfamily protein [more]
AT5G07410.14.2e-9546.30Pectin lyase-like superfamily protein [more]
AT1G69940.11.2e-9446.03Pectin lyase-like superfamily protein [more]
AT5G07420.11.7e-9144.51Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 85..370
e-value: 2.3E-67
score: 226.8
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 81..376
e-value: 4.4E-100
score: 337.1
NoneNo IPR availablePANTHERPTHR31321:SF30PECTINESTERASEcoord: 18..378
NoneNo IPR availablePANTHERPTHR31321ACYL-COA THIOESTER HYDROLASE YBHC-RELATEDcoord: 18..378
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 231..240
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 83..373

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0006809.1Tan0006809.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity