Tan0006718 (gene) Snake gourd v1

Overview


Name	Tan0006718
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	polygalacturonase inhibitor-like
Location	LG03: 48681585 .. 48682705 (+)
RNA-Seq Expression	Tan0006718
Synteny	Tan0006718

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTACAATTTACATCTAAACCATCATTTCAACAATGCTTCCTCACAGCCTGAAATCTCCACCTCCATCCACTTTCTTCCTCCTCCTCCTCCTCTTCCTCTTCTTCACCATTTCATTTGCAGAACGGTGCGATCCAAATGACAAAAAAGCCCTCCTCAACATAAAAAAAGCCTTCAACAATCCCTACGTCCTAGCCTCATGGAAACCCGAAGAAGACTGCTGCAGTTGGTACTGCGTCGACTGCGATGATAATTCCGACCGTATCATCTCCCTCAACATCATCACCGACGACGAACTCTCCGGCCAAATCCCACCGTCCGTCGGCGACCTCCCATTTCTCCAATATCTCACATTCCACAAACTCCCAAATCTGGTCGGACCCATCCAACCGACAATTGCCAAACTCCACAACCTCAAATTCCTCGACATCAGCTGGACAAATCTCTCCGGTTCCATCCCCGATTTCCTCAGCACCCTCTCCCACCTCTCTTACATTCGTCTCTCCTTCAACAATCTCACCGGAACCATCCCCGGATCCCTCTCAAAACTCCCAAAACTCGAATCTCTGCAATTAGACCGTAACAAATTGACCGGACCAATCCCAGATTCCTTCGGCTACTTGAAGGCCAAATCTCTCTACCTTTATCTTTCCCACAACAAACTTTCCGGCAAAATCCCACCTTCCTTATCCAAAGTGGACTTCAGTGCCATTGATTTCTCTCGGAACAAGCTGCAAGGCGATGCCTCCATGTTATTCGGACCAAACAAGAAGGCCTGGAAAGTTGATTTGTCAAGGAATTTGTTGGAGTTTAACATGTCCAAACTTGTGTTTTCCACCAGCCTCTTTACTTTGGATCTTAACCATAATAAGATCTTTGGGGGTTTGCCGCCGCAGATGACGAAACTCAGTAATCTTAATTCTTTCAATGTGAGTTACAATAGGCTTTGCGGTCCCATTCCGCTGGGTGGGAGCTTGCAGAGATTTGATGTTTATTCGTATTTTCATAATAAGTGTTTGTGTGGTGCACCACTCGCGAGCTGCAAATGATCGTAATTGCACTCGCTTTTATCCTGTTGGGTTTCAAGTTTCTATGAATAAAACTGATATATGAACTTCAAATT

mRNA sequence

ATTACAATTTACATCTAAACCATCATTTCAACAATGCTTCCTCACAGCCTGAAATCTCCACCTCCATCCACTTTCTTCCTCCTCCTCCTCCTCTTCCTCTTCTTCACCATTTCATTTGCAGAACGGTGCGATCCAAATGACAAAAAAGCCCTCCTCAACATAAAAAAAGCCTTCAACAATCCCTACGTCCTAGCCTCATGGAAACCCGAAGAAGACTGCTGCAGTTGGTACTGCGTCGACTGCGATGATAATTCCGACCGTATCATCTCCCTCAACATCATCACCGACGACGAACTCTCCGGCCAAATCCCACCGTCCGTCGGCGACCTCCCATTTCTCCAATATCTCACATTCCACAAACTCCCAAATCTGGTCGGACCCATCCAACCGACAATTGCCAAACTCCACAACCTCAAATTCCTCGACATCAGCTGGACAAATCTCTCCGGTTCCATCCCCGATTTCCTCAGCACCCTCTCCCACCTCTCTTACATTCGTCTCTCCTTCAACAATCTCACCGGAACCATCCCCGGATCCCTCTCAAAACTCCCAAAACTCGAATCTCTGCAATTAGACCGTAACAAATTGACCGGACCAATCCCAGATTCCTTCGGCTACTTGAAGGCCAAATCTCTCTACCTTTATCTTTCCCACAACAAACTTTCCGGCAAAATCCCACCTTCCTTATCCAAAGTGGACTTCAGTGCCATTGATTTCTCTCGGAACAAGCTGCAAGGCGATGCCTCCATGTTATTCGGACCAAACAAGAAGGCCTGGAAAGTTGATTTGTCAAGGAATTTGTTGGAGTTTAACATGTCCAAACTTGTGTTTTCCACCAGCCTCTTTACTTTGGATCTTAACCATAATAAGATCTTTGGGGGTTTGCCGCCGCAGATGACGAAACTCAGTAATCTTAATTCTTTCAATGTGAGTTACAATAGGCTTTGCGGTCCCATTCCGCTGGGTGGGAGCTTGCAGAGATTTGATGTTTATTCGTATTTTCATAATAAGTGTTTGTGTGGTGCACCACTCGCGAGCTGCAAATGATCGTAATTGCACTCGCTTTTATCCTGTTGGGTTTCAAGTTTCTATGAATAAAACTGATATATGAACTTCAAATT

Coding sequence (CDS)

ATGCTTCCTCACAGCCTGAAATCTCCACCTCCATCCACTTTCTTCCTCCTCCTCCTCCTCTTCCTCTTCTTCACCATTTCATTTGCAGAACGGTGCGATCCAAATGACAAAAAAGCCCTCCTCAACATAAAAAAAGCCTTCAACAATCCCTACGTCCTAGCCTCATGGAAACCCGAAGAAGACTGCTGCAGTTGGTACTGCGTCGACTGCGATGATAATTCCGACCGTATCATCTCCCTCAACATCATCACCGACGACGAACTCTCCGGCCAAATCCCACCGTCCGTCGGCGACCTCCCATTTCTCCAATATCTCACATTCCACAAACTCCCAAATCTGGTCGGACCCATCCAACCGACAATTGCCAAACTCCACAACCTCAAATTCCTCGACATCAGCTGGACAAATCTCTCCGGTTCCATCCCCGATTTCCTCAGCACCCTCTCCCACCTCTCTTACATTCGTCTCTCCTTCAACAATCTCACCGGAACCATCCCCGGATCCCTCTCAAAACTCCCAAAACTCGAATCTCTGCAATTAGACCGTAACAAATTGACCGGACCAATCCCAGATTCCTTCGGCTACTTGAAGGCCAAATCTCTCTACCTTTATCTTTCCCACAACAAACTTTCCGGCAAAATCCCACCTTCCTTATCCAAAGTGGACTTCAGTGCCATTGATTTCTCTCGGAACAAGCTGCAAGGCGATGCCTCCATGTTATTCGGACCAAACAAGAAGGCCTGGAAAGTTGATTTGTCAAGGAATTTGTTGGAGTTTAACATGTCCAAACTTGTGTTTTCCACCAGCCTCTTTACTTTGGATCTTAACCATAATAAGATCTTTGGGGGTTTGCCGCCGCAGATGACGAAACTCAGTAATCTTAATTCTTTCAATGTGAGTTACAATAGGCTTTGCGGTCCCATTCCGCTGGGTGGGAGCTTGCAGAGATTTGATGTTTATTCGTATTTTCATAATAAGTGTTTGTGTGGTGCACCACTCGCGAGCTGCAAATGA

Protein sequence

MLPHSLKSPPPSTFFLLLLLFLFFTISFAERCDPNDKKALLNIKKAFNNPYVLASWKPEEDCCSWYCVDCDDNSDRIISLNIITDDELSGQIPPSVGDLPFLQYLTFHKLPNLVGPIQPTIAKLHNLKFLDISWTNLSGSIPDFLSTLSHLSYIRLSFNNLTGTIPGSLSKLPKLESLQLDRNKLTGPIPDSFGYLKAKSLYLYLSHNKLSGKIPPSLSKVDFSAIDFSRNKLQGDASMLFGPNKKAWKVDLSRNLLEFNMSKLVFSTSLFTLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPLGGSLQRFDVYSYFHNKCLCGAPLASCK

Homology

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BLAST of Tan0006718 vs. ExPASy Swiss-Prot
Match: Q05091 (Polygalacturonase inhibitor OS=Pyrus communis OX=23211 GN=PGIP PE=1 SV=1)

HSP 1 Score: 419.1 bits (1076), Expect = 4.8e-116
Identity = 207/327 (63.30%), Postives = 248/327 (75.84%), Query Frame = 0


Query: 12  STFFLLLLLF-LFFTISFAERCDPNDKKALLNIKKAFNNPYVLASWKPEEDCCSWYCVDC 71
           STF  L LLF      + ++ C+P+DKK LL IKKAF +PYVLASWK + DCC WYCV C
Sbjct: 6   STFLSLTLLFSSVLNPALSDLCNPDDKKVLLQIKKAFGDPYVLASWKSDTDCCDWYCVTC 65

Query: 72  DDNSDRIISLNIITDDELSGQIPPSVGDLPFLQYLTFHKLPNLVGPIQPTIAKLHNLKFL 131
           D  ++RI SL I    ++SGQIP  VGDLP+L+ L FHK PNL GPIQP IAKL  LK L
Sbjct: 66  DSTTNRINSLTIFA-GQVSGQIPALVGDLPYLETLEFHKQPNLTGPIQPAIAKLKGLKSL 125

Query: 132 DISWTNLSGSIPDFLSTLSHLSYIRLSFNNLTGTIPGSLSKLPKLESLQLDRNKLTGPIP 191
            +SWTNLSGS+PDFLS L +L+++ LSFNNLTG IP SLS+LP L +L+LDRNKLTG IP
Sbjct: 126 RLSWTNLSGSVPDFLSQLKNLTFLDLSFNNLTGAIPSSLSELPNLGALRLDRNKLTGHIP 185

Query: 192 DSFGYLKAKSLYLYLSHNKLSGKIPPSLSKVDFSAIDFSRNKLQGDASMLFGPNKKAWKV 251
            SFG        LYLSHN+LSG IP S +++DF++ID SRNKL+GDAS++FG NK    V
Sbjct: 186 ISFGQFIGNVPDLYLSHNQLSGNIPTSFAQMDFTSIDLSRNKLEGDASVIFGLNKTTQIV 245

Query: 252 DLSRNLLEFNMSKLVFSTSLFTLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPL 311
           DLSRNLLEFN+SK+ F TSL +LD+NHNKI+G +P + T+L N    NVSYNRLCG IP+
Sbjct: 246 DLSRNLLEFNLSKVEFPTSLTSLDINHNKIYGSIPVEFTQL-NFQFLNVSYNRLCGQIPV 305

Query: 312 GGSLQRFDVYSYFHNKCLCGAPLASCK 338
           GG LQ FD YSYFHN+CLCGAPL SCK
Sbjct: 306 GGKLQSFDEYSYFHNRCLCGAPLPSCK 330

[Close]

BLAST of Tan0006718 vs. ExPASy Swiss-Prot
Match: Q9M5J8 (Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PGIP2 PE=2 SV=2)

HSP 1 Score: 390.2 bits (1001), Expect = 2.4e-107
Identity = 196/327 (59.94%), Postives = 234/327 (71.56%), Query Frame = 0


Query: 13  TFFLLLLLFLFFTISFAERCDPNDKKALLNIKKAFNNPYVLASWKPEEDCCSWYCVDCDD 72
           T FLLL   L  T    + C  +DK  LL IKK+ NNPY LASW P+ DCCSWYC++C D
Sbjct: 6   TLFLLLSTLLLTTSLAKDLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYCLECGD 65

Query: 73  N--SDRIISLNIITDDELSGQIPPSVGDLPFLQYLTFHKLPNLVGPIQPTIAKLHNLKFL 132
              + R+ SL II D E+SGQIPP VGDLP+L  L F KL NL G IQPTIAKL NL FL
Sbjct: 66  ATVNHRVTSL-IIQDGEISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLKNLTFL 125

Query: 133 DISWTNLSGSIPDFLSTLSHLSYIRLSFNNLTGTIPGSLSKLPKLESLQLDRNKLTGPIP 192
            +SWTNL+G +P+FLS L +L YI LSFN+L+G+IP SLS L KLE L+L RNKLTGPIP
Sbjct: 126 RLSWTNLTGPVPEFLSQLKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKLTGPIP 185

Query: 193 DSFGYLKAKSLYLYLSHNKLSGKIPPSLSKVDFSAIDFSRNKLQGDASMLFGPNKKAWKV 252
           +SFG    K   L+LSHN+LSG IP SL   DF  ID SRNKLQGDAS+LFG  K  W V
Sbjct: 186 ESFGTFSGKVPSLFLSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKKTTWIV 245

Query: 253 DLSRNLLEFNMSKLVFSTSLFTLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPL 312
           D+SRN+ +F++SK+  + +L  LD+NHN I G +P + +K +     NVSYNRLCG IP 
Sbjct: 246 DISRNMFQFDLSKVKLAKTLNNLDMNHNGITGSIPAEWSK-AYFQLLNVSYNRLCGRIPK 305

Query: 313 GGSLQRFDVYSYFHNKCLCGAPLASCK 338
           G  +QRFD YS+FHNKCLCGAPL SCK
Sbjct: 306 GEYIQRFDSYSFFHNKCLCGAPLPSCK 330

[Close]

BLAST of Tan0006718 vs. ExPASy Swiss-Prot
Match: Q9M5J9 (Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PGIP1 PE=1 SV=1)

HSP 1 Score: 389.8 bits (1000), Expect = 3.1e-107
Identity = 192/324 (59.26%), Postives = 235/324 (72.53%), Query Frame = 0


Query: 17  LLLLFLFFTISFA-ERCDPNDKKALLNIKKAFNNPYVLASWKPEEDCCSWYCVDCDDN-- 76
           LL LF F T   + + C+ NDK  LL IKK+ NNPY LASW P+ DCCSWYC++C D   
Sbjct: 9   LLFLFTFLTTCLSKDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYCLECGDATV 68

Query: 77  SDRIISLNIITDDELSGQIPPSVGDLPFLQYLTFHKLPNLVGPIQPTIAKLHNLKFLDIS 136
           + R+ +L I +  ++SGQIP  VGDLP+L+ L F KL NL G IQPTIAKL NL+ L +S
Sbjct: 69  NHRVTALTIFS-GQISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLKNLRMLRLS 128

Query: 137 WTNLSGSIPDFLSTLSHLSYIRLSFNNLTGTIPGSLSKLPKLESLQLDRNKLTGPIPDSF 196
           WTNL+G IPDF+S L +L ++ LSFN+L+G+IP SLS LPK+ +L+L RNKLTG IP+SF
Sbjct: 129 WTNLTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKLTGSIPESF 188

Query: 197 GYLKAKSLYLYLSHNKLSGKIPPSLSKVDFSAIDFSRNKLQGDASMLFGPNKKAWKVDLS 256
           G        L LSHN+LSG IP SL  +DF+ ID SRNKLQGDASMLFG NK  W +DLS
Sbjct: 189 GSFPGTVPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNKTTWSIDLS 248

Query: 257 RNLLEFNMSKLVFSTSLFTLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPLGGS 316
           RN+ +F++SK+    +L  LDLNHN I G +P Q T+ + L  FNVSYN+LCG IP GG 
Sbjct: 249 RNMFQFDISKVDIPKTLGILDLNHNGITGNIPVQWTE-APLQFFNVSYNKLCGHIPTGGK 308

Query: 317 LQRFDVYSYFHNKCLCGAPLASCK 338
           LQ FD YSYFHNKCLCGAPL  CK
Sbjct: 309 LQTFDSYSYFHNKCLCGAPLEICK 330

[Close]

BLAST of Tan0006718 vs. ExPASy Swiss-Prot
Match: A7PW81 (Polygalacturonase inhibitor OS=Vitis vinifera OX=29760 GN=pgip PE=1 SV=1)

HSP 1 Score: 387.5 bits (994), Expect = 1.5e-106
Identity = 195/326 (59.82%), Postives = 233/326 (71.47%), Query Frame = 0


Query: 12  STFFLLLLLFLFFTISFAERCDPNDKKALLNIKKAFNNPYVLASWKPEEDCCSWYCVDCD 71
           S+  LL+LL      S +ERC+P DKK LL IKKA +NPY+LASW P  DCC WYCV+CD
Sbjct: 10  SSSLLLVLLATRPCPSLSERCNPKDKKVLLQIKKALDNPYILASWNPNTDCCGWYCVECD 69

Query: 72  DNSDRIISLNIITDDELSGQIPPSVGDLPFLQYLTFHKLPNLVGPIQPTIAKLHNLKFLD 131
             + RI SL I +  +LSGQIP +VGDLPFL+ L F KL NL G I P IAKL +LK + 
Sbjct: 70  LTTHRINSLTIFS-GQLSGQIPDAVGDLPFLETLIFRKLSNLTGQIPPAIAKLKHLKMVR 129

Query: 132 ISWTNLSGSIPDFLSTLSHLSYIRLSFNNLTGTIPGSLSKLPKLESLQLDRNKLTGPIPD 191
           +SWTNLSG +P F S L +L+Y+ LSFNNL+G IPGSLS LP L +L LDRN LTGPIPD
Sbjct: 130 LSWTNLSGPVPAFFSELKNLTYLDLSFNNLSGPIPGSLSLLPNLGALHLDRNHLTGPIPD 189

Query: 192 SFGYLKAKSLYLYLSHNKLSGKIPPSLSKVDFSAIDFSRNKLQGDASMLFGPNKKAWKVD 251
           SFG     +  L+LSHN+LSGKIP S    D + +D SRNKL+GD S+ F  NK    VD
Sbjct: 190 SFGKFAGSTPGLHLSHNQLSGKIPYSFRGFDPNVMDLSRNKLEGDLSIFFNANKSTQIVD 249

Query: 252 LSRNLLEFNMSKLVFSTSLFTLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPLG 311
            SRNL +F++S++ F  SL +LDL+HNKI G LP  MT L +L   NVSYNRLCG IP+G
Sbjct: 250 FSRNLFQFDLSRVEFPKSLTSLDLSHNKIAGSLPEMMTSL-DLQFLNVSYNRLCGKIPVG 309

Query: 312 GSLQRFDVYSYFHNKCLCGAPLASCK 338
           G LQ FD  SYFHN+CLCGAPL SCK
Sbjct: 310 GKLQSFDYDSYFHNRCLCGAPLQSCK 333

[Close]

BLAST of Tan0006718 vs. ExPASy Swiss-Prot
Match: Q9LH52 (Leucine-rich repeat protein FLOR 1 OS=Arabidopsis thaliana OX=3702 GN=FLR1 PE=1 SV=1)

HSP 1 Score: 319.3 bits (817), Expect = 5.2e-86
Identity = 165/316 (52.22%), Postives = 214/316 (67.72%), Query Frame = 0


Query: 22  LFFTISFAERCDPNDKKALLNIKKAFNNPYVLASWKPEEDCCS-WYCVDCDDNSDRIISL 81
           LF T+  +  C  NDK ALL IKKA  NP +L+SW P  DCC+ W  V+C +   R+  L
Sbjct: 14  LFITLPSSYSCTENDKNALLQIKKALGNPPLLSSWNPRTDCCTGWTGVECTNR--RVTGL 73

Query: 82  NIITDDELSGQIPPSVGDLPFLQYLTFHKLPNLVGPIQPTIAKLHNLKFLDISWTNLSGS 141
           + +T  E+SGQI   +GDL  L+ L F  LP+L G I  TI KL NL  L +  T+LSG 
Sbjct: 74  S-VTSGEVSGQISYQIGDLVDLRTLDFSYLPHLTGNIPRTITKLKNLNTLYLKHTSLSGP 133

Query: 142 IPDFLSTLSHLSYIRLSFNNLTGTIPGSLSKLPKLESLQLDRNKLTGPIPDSFGYLKAKS 201
           IPD++S L  L+++ LSFN  TG IPGSLS++PKLE++Q++ NKLTG IP+SFG      
Sbjct: 134 IPDYISELKSLTFLDLSFNQFTGPIPGSLSQMPKLEAIQINDNKLTGSIPNSFGSFVGNV 193

Query: 202 LYLYLSHNKLSGKIPPSLSKVDFSAIDFSRNKLQGDASMLFGPNKKAWKVDLSRNLLEFN 261
             LYLS+NKLSGKIP SLSK DF+A+D S N  +GDA M FG NK   +VDLSRN+  F+
Sbjct: 194 PNLYLSNNKLSGKIPESLSKYDFNAVDLSGNGFEGDAFMFFGRNKTTVRVDLSRNMFNFD 253

Query: 262 MSKLVFSTSLFTLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPLGGSLQRFDVY 321
           + K+ F+ S+ +LDL+ N I+G +PP +TKL +L  FNVS N LCG IP GG LQ F+  
Sbjct: 254 LVKVKFARSIVSLDLSQNHIYGKIPPALTKL-HLEHFNVSDNHLCGKIPSGGLLQTFEPS 313

Query: 322 SYFHNKCLCGAPLASC 337
           ++ HN CLCG PL +C
Sbjct: 314 AFAHNICLCGTPLKAC 325

[Close]

BLAST of Tan0006718 vs. NCBI nr
Match: XP_023517556.1 (polygalacturonase inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 555.1 bits (1429), Expect = 4.3e-154
Identity = 269/337 (79.82%), Postives = 294/337 (87.24%), Query Frame = 0


Query: 1   MLPHSLKSPPPSTFFLLLLLFLFFTISFAERCDPNDKKALLNIKKAFNNPYVLASWKPEE 60
           M+  SLKSP  ST   LL  FLFFTISFAE C+PNDKKALLNIKKAFNN Y  ASWKPEE
Sbjct: 1   MVRLSLKSPLSST-ISLLFFFLFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEE 60

Query: 61  DCCSWYCVDCDDNSDRIISLNIITDDELSGQIPPSVGDLPFLQYLTFHKLPNLVGPIQPT 120
           DCC+WY VDCD+NS RIISL+I TD+E+SGQIPP +GDLPFLQ L  HKLPNLVGPIQPT
Sbjct: 61  DCCTWYSVDCDENSHRIISLDISTDNEISGQIPPYIGDLPFLQSLMLHKLPNLVGPIQPT 120

Query: 121 IAKLHNLKFLDISWTNLSGSIPDFLSTLSHLSYIRLSFNNLTGTIPGSLSKLPKLESLQL 180
           IAKLHNLK LDISWTN+SG+IPDFLS+LS L+YIRLSF+NL+G IP SLSKLP LE LQL
Sbjct: 121 IAKLHNLKHLDISWTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPSLEFLQL 180

Query: 181 DRNKLTGPIPDSFGYLKAKSLYLYLSHNKLSGKIPPSLSKVDFSAIDFSRNKLQGDASML 240
           DR+KLTG IP SFG  KAK  YLYLSHNKLSGKIPPSLS+VDF ++D SRNKL+GDASM+
Sbjct: 181 DRSKLTGQIPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMI 240

Query: 241 FGPNKKAWKVDLSRNLLEFNMSKLVFSTSLFTLDLNHNKIFGGLPPQMTKLSNLNSFNVS 300
           FG ++  WKVDLSRNLLEF+MSK+VFSTSLF LDLNHNKI+GGLPPQMTKL NLNSFNVS
Sbjct: 241 FGEHRHTWKVDLSRNLLEFDMSKVVFSTSLFMLDLNHNKIYGGLPPQMTKLGNLNSFNVS 300

Query: 301 YNRLCGPIPLGGSLQRFDVYSYFHNKCLCGAPLASCK 338
           YNRLCG IP GG LQRFDVYSYFHNKCLCGAPL SCK
Sbjct: 301 YNRLCGQIPKGGELQRFDVYSYFHNKCLCGAPLESCK 336

[Close]

BLAST of Tan0006718 vs. NCBI nr
Match: KAG6595655.1 (Polygalacturonase inhibitor, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 551.6 bits (1420), Expect = 4.7e-153
Identity = 267/337 (79.23%), Postives = 292/337 (86.65%), Query Frame = 0


Query: 1   MLPHSLKSPPPSTFFLLLLLFLFFTISFAERCDPNDKKALLNIKKAFNNPYVLASWKPEE 60
           ML  +LKSP P  FF     FLFFTISFAE C+PNDKKALLNIKKAFNN Y  ASWKPEE
Sbjct: 1   MLVLTLKSPLPLLFF-----FLFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEE 60

Query: 61  DCCSWYCVDCDDNSDRIISLNIITDDELSGQIPPSVGDLPFLQYLTFHKLPNLVGPIQPT 120
           DCC+WY V CD+NS RIISL+I TD+E+SGQIPP VGDLPFLQ L  HKLPNLVGPIQP 
Sbjct: 61  DCCTWYSVHCDENSHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPA 120

Query: 121 IAKLHNLKFLDISWTNLSGSIPDFLSTLSHLSYIRLSFNNLTGTIPGSLSKLPKLESLQL 180
           +AKLHNLK LDISWTN+SG+IPDFLS+LS L+YIRLSF+NL+G IP SLSKLP LE LQL
Sbjct: 121 LAKLHNLKHLDISWTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQL 180

Query: 181 DRNKLTGPIPDSFGYLKAKSLYLYLSHNKLSGKIPPSLSKVDFSAIDFSRNKLQGDASML 240
           DR+KLTG IP SFG  KAK  YLYLSHNKLSGKIPPSLS+VDF ++D SRNKL+GDASM+
Sbjct: 181 DRSKLTGQIPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMI 240

Query: 241 FGPNKKAWKVDLSRNLLEFNMSKLVFSTSLFTLDLNHNKIFGGLPPQMTKLSNLNSFNVS 300
           FG ++  WKVDLSRNLLEF+MSK+VFSTSLF LDLNHNKI+GGLPPQMTKLSNLNSFNVS
Sbjct: 241 FGAHRHTWKVDLSRNLLEFDMSKVVFSTSLFMLDLNHNKIYGGLPPQMTKLSNLNSFNVS 300

Query: 301 YNRLCGPIPLGGSLQRFDVYSYFHNKCLCGAPLASCK 338
           YNRLCG IP GG LQRFDVYSYFHNKCLCGAPL SCK
Sbjct: 301 YNRLCGQIPKGGELQRFDVYSYFHNKCLCGAPLESCK 332

[Close]

BLAST of Tan0006718 vs. NCBI nr
Match: XP_022966387.1 (polygalacturonase inhibitor-like [Cucurbita maxima])

HSP 1 Score: 551.2 bits (1419), Expect = 6.2e-153
Identity = 267/337 (79.23%), Postives = 292/337 (86.65%), Query Frame = 0


Query: 1   MLPHSLKSPPPSTFFLLLLLFLFFTISFAERCDPNDKKALLNIKKAFNNPYVLASWKPEE 60
           ML  +LKSP P  FF     F+FFTISFAE C+PNDKKALLNIKKAFNN Y  ASWKPEE
Sbjct: 1   MLVLTLKSPLPLLFF-----FIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEE 60

Query: 61  DCCSWYCVDCDDNSDRIISLNIITDDELSGQIPPSVGDLPFLQYLTFHKLPNLVGPIQPT 120
           DCC+WY V+CD+NS RIISL+I TD+E+SGQIPP VGDLPFLQ L  HKLPNLVGPIQP 
Sbjct: 61  DCCTWYSVECDENSHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPA 120

Query: 121 IAKLHNLKFLDISWTNLSGSIPDFLSTLSHLSYIRLSFNNLTGTIPGSLSKLPKLESLQL 180
           IAKLHNLK LDISWTN+SG+IPDFLS+LS L+YIRLSF+NL+G IP SLSKLP LE LQL
Sbjct: 121 IAKLHNLKHLDISWTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQL 180

Query: 181 DRNKLTGPIPDSFGYLKAKSLYLYLSHNKLSGKIPPSLSKVDFSAIDFSRNKLQGDASML 240
           DR+KLTG IP SFG  KAK  YLYLSHNKLSGKIPPSLS+VDF ++D SRNKL+GDASM+
Sbjct: 181 DRSKLTGLIPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMI 240

Query: 241 FGPNKKAWKVDLSRNLLEFNMSKLVFSTSLFTLDLNHNKIFGGLPPQMTKLSNLNSFNVS 300
           FG ++  WKVDLSRNLLEF+MSK+VFS SLF LDLNHNKIFGGLPPQMTKLSNLNSFNVS
Sbjct: 241 FGAHRNTWKVDLSRNLLEFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVS 300

Query: 301 YNRLCGPIPLGGSLQRFDVYSYFHNKCLCGAPLASCK 338
           YNRLCG IP GG LQRFDVYSYFHNKCLCGAPL SCK
Sbjct: 301 YNRLCGQIPKGGELQRFDVYSYFHNKCLCGAPLESCK 332

[Close]

BLAST of Tan0006718 vs. NCBI nr
Match: XP_022925061.1 (polygalacturonase inhibitor-like [Cucurbita moschata])

HSP 1 Score: 549.7 bits (1415), Expect = 1.8e-152
Identity = 265/337 (78.64%), Postives = 293/337 (86.94%), Query Frame = 0


Query: 1   MLPHSLKSPPPSTFFLLLLLFLFFTISFAERCDPNDKKALLNIKKAFNNPYVLASWKPEE 60
           ML  +LKSP P  FF     FLFFTISFAE C+PNDKKALLNIKKAFNN Y  ASWKPEE
Sbjct: 1   MLVLTLKSPLPLLFF-----FLFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEE 60

Query: 61  DCCSWYCVDCDDNSDRIISLNIITDDELSGQIPPSVGDLPFLQYLTFHKLPNLVGPIQPT 120
           DCC+WY V+CD+NS RIISL+I TD+E+SGQIPP VGDLPFLQ L  HKLPNLVGPIQP 
Sbjct: 61  DCCTWYSVECDENSHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPA 120

Query: 121 IAKLHNLKFLDISWTNLSGSIPDFLSTLSHLSYIRLSFNNLTGTIPGSLSKLPKLESLQL 180
           +AKLHNLK LDISWTN+SG+IPDFLS+L+ L+YIRLSF+NL+G IP SLSKLP L+ LQL
Sbjct: 121 LAKLHNLKHLDISWTNISGTIPDFLSSLTKLTYIRLSFSNLSGAIPSSLSKLPNLKFLQL 180

Query: 181 DRNKLTGPIPDSFGYLKAKSLYLYLSHNKLSGKIPPSLSKVDFSAIDFSRNKLQGDASML 240
           DR+KLTG IP SFG  KAK  YLYLSHNKLSGKIPPSLS+VDF ++D SRNKL+GDASM+
Sbjct: 181 DRSKLTGQIPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMI 240

Query: 241 FGPNKKAWKVDLSRNLLEFNMSKLVFSTSLFTLDLNHNKIFGGLPPQMTKLSNLNSFNVS 300
           FG ++  WKVDLSRNLLEF+MSK+VFSTSLF LDLNHNKI+GGLPPQMTKLSNLNSFNVS
Sbjct: 241 FGVHRHTWKVDLSRNLLEFDMSKVVFSTSLFMLDLNHNKIYGGLPPQMTKLSNLNSFNVS 300

Query: 301 YNRLCGPIPLGGSLQRFDVYSYFHNKCLCGAPLASCK 338
           YNRLCG IP GG LQRFDVYSYFHNKCLCGAPL SCK
Sbjct: 301 YNRLCGQIPKGGELQRFDVYSYFHNKCLCGAPLESCK 332

[Close]

BLAST of Tan0006718 vs. NCBI nr
Match: KAG6595656.1 (Polygalacturonase inhibitor, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 524.6 bits (1350), Expect = 6.2e-145
Identity = 259/337 (76.85%), Postives = 288/337 (85.46%), Query Frame = 0


Query: 1   MLPHSLKSPPPSTFFLLLLLFLFFTISFAERCDPNDKKALLNIKKAFNNPYVLASWKPEE 60
           M+  SLKSP  ST F LLL+F+FFTISFAE C+PNDKKALLNIK+AFNN Y  ASWKPEE
Sbjct: 1   MVRLSLKSPLLST-FSLLLIFIFFTISFAELCNPNDKKALLNIKEAFNNAYYFASWKPEE 60

Query: 61  DCCSWYCVDCDDNSDRIISLNIITDDELSGQIPPSVGDLPFLQYLTFHKLPNLVGPIQPT 120
           DCC+WY V CD+ S RIISL I  D ++SGQIPP VGDLP+LQYLTFHKLPNLVGPIQP 
Sbjct: 61  DCCTWYSVQCDEISHRIISLTISVDKQISGQIPPHVGDLPYLQYLTFHKLPNLVGPIQPA 120

Query: 121 IAKLHNLKFLDISWTNLSGSIPDFLSTLSHLSYIRLSFNNLTGTIPGSLSKLPKLESLQL 180
           IAKLHNLK LDISWT +SG+IPDFLS+LS+LSYI LSF+NL+G IP SLSKLP L SLQL
Sbjct: 121 IAKLHNLKHLDISWTKISGTIPDFLSSLSNLSYISLSFSNLSGAIPSSLSKLPNLSSLQL 180

Query: 181 DRNKLTGPIPDSFGYLKAKSLYLYLSHNKLSGKIPPSLSKVDFSAIDFSRNKLQGDASML 240
           DR+KLTG IPDSFG  KA++  LYLSHNKL+GKIPPSLSKVDFSAID SRNKL+GDA M+
Sbjct: 181 DRSKLTGQIPDSFGDFKAENFNLYLSHNKLTGKIPPSLSKVDFSAIDLSRNKLEGDAYMI 240

Query: 241 FGPNKKAWKVDLSRNLLEFNMSKLVFSTSLFTLDLNHNKIFGGLPPQMTKLSNLNSFNVS 300
           FG +KKAW VDLSRNLL F+MSK+VFST+LF LD+NHNKIFGGLPPQM+KLS L S NVS
Sbjct: 241 FGAHKKAWNVDLSRNLLAFDMSKVVFSTNLFMLDVNHNKIFGGLPPQMSKLS-LTSLNVS 300

Query: 301 YNRLCGPIPLGGSLQRFDVYSYFHNKCLCGAPLASCK 338
           YNRLCG IP G  L+RF V SYFHNKCLCG PL SCK
Sbjct: 301 YNRLCGQIPKGKKLKRFGVDSYFHNKCLCGKPLESCK 335

[Close]

BLAST of Tan0006718 vs. ExPASy TrEMBL
Match: A0A6J1HMU3 (polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111466050 PE=4 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 3.0e-153
Identity = 267/337 (79.23%), Postives = 292/337 (86.65%), Query Frame = 0


Query: 1   MLPHSLKSPPPSTFFLLLLLFLFFTISFAERCDPNDKKALLNIKKAFNNPYVLASWKPEE 60
           ML  +LKSP P  FF     F+FFTISFAE C+PNDKKALLNIKKAFNN Y  ASWKPEE
Sbjct: 1   MLVLTLKSPLPLLFF-----FIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEE 60

Query: 61  DCCSWYCVDCDDNSDRIISLNIITDDELSGQIPPSVGDLPFLQYLTFHKLPNLVGPIQPT 120
           DCC+WY V+CD+NS RIISL+I TD+E+SGQIPP VGDLPFLQ L  HKLPNLVGPIQP 
Sbjct: 61  DCCTWYSVECDENSHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPA 120

Query: 121 IAKLHNLKFLDISWTNLSGSIPDFLSTLSHLSYIRLSFNNLTGTIPGSLSKLPKLESLQL 180
           IAKLHNLK LDISWTN+SG+IPDFLS+LS L+YIRLSF+NL+G IP SLSKLP LE LQL
Sbjct: 121 IAKLHNLKHLDISWTNISGTIPDFLSSLSKLTYIRLSFSNLSGAIPSSLSKLPNLEFLQL 180

Query: 181 DRNKLTGPIPDSFGYLKAKSLYLYLSHNKLSGKIPPSLSKVDFSAIDFSRNKLQGDASML 240
           DR+KLTG IP SFG  KAK  YLYLSHNKLSGKIPPSLS+VDF ++D SRNKL+GDASM+
Sbjct: 181 DRSKLTGLIPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMI 240

Query: 241 FGPNKKAWKVDLSRNLLEFNMSKLVFSTSLFTLDLNHNKIFGGLPPQMTKLSNLNSFNVS 300
           FG ++  WKVDLSRNLLEF+MSK+VFS SLF LDLNHNKIFGGLPPQMTKLSNLNSFNVS
Sbjct: 241 FGAHRNTWKVDLSRNLLEFDMSKVVFSRSLFMLDLNHNKIFGGLPPQMTKLSNLNSFNVS 300

Query: 301 YNRLCGPIPLGGSLQRFDVYSYFHNKCLCGAPLASCK 338
           YNRLCG IP GG LQRFDVYSYFHNKCLCGAPL SCK
Sbjct: 301 YNRLCGQIPKGGELQRFDVYSYFHNKCLCGAPLESCK 332

[Close]

BLAST of Tan0006718 vs. ExPASy TrEMBL
Match: A0A6J1EB58 (polygalacturonase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111432422 PE=4 SV=1)

HSP 1 Score: 549.7 bits (1415), Expect = 8.7e-153
Identity = 265/337 (78.64%), Postives = 293/337 (86.94%), Query Frame = 0


Query: 1   MLPHSLKSPPPSTFFLLLLLFLFFTISFAERCDPNDKKALLNIKKAFNNPYVLASWKPEE 60
           ML  +LKSP P  FF     FLFFTISFAE C+PNDKKALLNIKKAFNN Y  ASWKPEE
Sbjct: 1   MLVLTLKSPLPLLFF-----FLFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKPEE 60

Query: 61  DCCSWYCVDCDDNSDRIISLNIITDDELSGQIPPSVGDLPFLQYLTFHKLPNLVGPIQPT 120
           DCC+WY V+CD+NS RIISL+I TD+E+SGQIPP VGDLPFLQ L  HKLPNLVGPIQP 
Sbjct: 61  DCCTWYSVECDENSHRIISLDISTDNEISGQIPPYVGDLPFLQSLMLHKLPNLVGPIQPA 120

Query: 121 IAKLHNLKFLDISWTNLSGSIPDFLSTLSHLSYIRLSFNNLTGTIPGSLSKLPKLESLQL 180
           +AKLHNLK LDISWTN+SG+IPDFLS+L+ L+YIRLSF+NL+G IP SLSKLP L+ LQL
Sbjct: 121 LAKLHNLKHLDISWTNISGTIPDFLSSLTKLTYIRLSFSNLSGAIPSSLSKLPNLKFLQL 180

Query: 181 DRNKLTGPIPDSFGYLKAKSLYLYLSHNKLSGKIPPSLSKVDFSAIDFSRNKLQGDASML 240
           DR+KLTG IP SFG  KAK  YLYLSHNKLSGKIPPSLS+VDF ++D SRNKL+GDASM+
Sbjct: 181 DRSKLTGQIPYSFGDFKAKGFYLYLSHNKLSGKIPPSLSRVDFESVDLSRNKLEGDASMI 240

Query: 241 FGPNKKAWKVDLSRNLLEFNMSKLVFSTSLFTLDLNHNKIFGGLPPQMTKLSNLNSFNVS 300
           FG ++  WKVDLSRNLLEF+MSK+VFSTSLF LDLNHNKI+GGLPPQMTKLSNLNSFNVS
Sbjct: 241 FGVHRHTWKVDLSRNLLEFDMSKVVFSTSLFMLDLNHNKIYGGLPPQMTKLSNLNSFNVS 300

Query: 301 YNRLCGPIPLGGSLQRFDVYSYFHNKCLCGAPLASCK 338
           YNRLCG IP GG LQRFDVYSYFHNKCLCGAPL SCK
Sbjct: 301 YNRLCGQIPKGGELQRFDVYSYFHNKCLCGAPLESCK 332

[Close]

BLAST of Tan0006718 vs. ExPASy TrEMBL
Match: A0A6J1HP52 (polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111466048 PE=4 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 1.9e-144
Identity = 258/337 (76.56%), Postives = 284/337 (84.27%), Query Frame = 0


Query: 1   MLPHSLKSPPPSTFFLLLLLFLFFTISFAERCDPNDKKALLNIKKAFNNPYVLASWKPEE 60
           M+  SLKSP  ST F LL  F+FFTISFAE C+PNDKKALLNIKKAFNN Y  ASWK EE
Sbjct: 1   MVRLSLKSPLLST-FSLLFFFIFFTISFAELCNPNDKKALLNIKKAFNNAYYFASWKAEE 60

Query: 61  DCCSWYCVDCDDNSDRIISLNIITDDELSGQIPPSVGDLPFLQYLTFHKLPNLVGPIQPT 120
           DCC+WY V CD+NS RIISL +  D ++SGQIPP VG+LPFLQYLTFHKLPNLVGPIQP 
Sbjct: 61  DCCTWYSVQCDENSHRIISLTLAVDKQISGQIPPHVGELPFLQYLTFHKLPNLVGPIQPA 120

Query: 121 IAKLHNLKFLDISWTNLSGSIPDFLSTLSHLSYIRLSFNNLTGTIPGSLSKLPKLESLQL 180
           IAKLHNLK LDISWT +SG+IPDFLS+LS+LSYI LSF+NL+G IP SLSKLP L  LQL
Sbjct: 121 IAKLHNLKHLDISWTKISGTIPDFLSSLSNLSYISLSFSNLSGAIPSSLSKLPNLSYLQL 180

Query: 181 DRNKLTGPIPDSFGYLKAKSLYLYLSHNKLSGKIPPSLSKVDFSAIDFSRNKLQGDASML 240
           DR+KLTG IPDSFG  KA++  LYLSHNKLSGKIPPSLSKVDFSAID SRNKL+GDA M+
Sbjct: 181 DRSKLTGQIPDSFGDFKAENFNLYLSHNKLSGKIPPSLSKVDFSAIDLSRNKLEGDAYMI 240

Query: 241 FGPNKKAWKVDLSRNLLEFNMSKLVFSTSLFTLDLNHNKIFGGLPPQMTKLSNLNSFNVS 300
           FG  KKAW VDLSRNLL F+MSK+VFST+LF LD+NHNKIFGGLPPQM+KLS L S NVS
Sbjct: 241 FGAQKKAWNVDLSRNLLAFDMSKVVFSTNLFMLDVNHNKIFGGLPPQMSKLS-LTSLNVS 300

Query: 301 YNRLCGPIPLGGSLQRFDVYSYFHNKCLCGAPLASCK 338
           YNRLCG IP G  L+RF V SYFHNKCLCG PL SCK
Sbjct: 301 YNRLCGQIPKGKKLKRFGVDSYFHNKCLCGKPLESCK 335

[Close]

BLAST of Tan0006718 vs. ExPASy TrEMBL
Match: Q7X9Q7 (Polygalacturonase-inhibiting protein OS=Cucumis melo OX=3656 GN=LOC103499550 PE=2 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 3.9e-129
Identity = 227/322 (70.50%), Postives = 254/322 (78.88%), Query Frame = 0


Query: 16  LLLLLFLFFTISFAERCDPNDKKALLNIKKAFNNPYVLASWKPEEDCCSWYCVDCDDNSD 75
           LLLLLF FFT+SFAE C PNDK+ LLNIKKAFNNPY+L SWKPEEDCC+WYCV+CD  S 
Sbjct: 6   LLLLLFFFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDLKSH 65

Query: 76  RIISLNIITDDELSGQIPPSVGDLPFLQYLTFHKLPNLVGPIQPTIAKLHNLKFLDISWT 135
           RII+L I  DDELSG IPP VGDLPFL+ L FHKLPNL GPI PTIAKLHNLK+LD+SW 
Sbjct: 66  RIIALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLKYLDLSWN 125

Query: 136 NLSGSIPDFLSTLSHLSYIRLSFNNLTGTIPGSLSKLPKLESLQLDRNKLTGPIPDSFGY 195
            LSG IP FL +LS+L  + LSFN  TG+IP SL+ L +L +L LDRNKLTGPIPDSFG 
Sbjct: 126 GLSGPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPDSFGN 185

Query: 196 LKAKSLYLYLSHNKLSGKIPPSLSKVDFSAIDFSRNKLQGDASMLFGPNKKAWKVDLSRN 255
            K K  YLYLSHN+LSGKIP S+ KVDF+ ID SRNKL GD S++FG  K    VDLSRN
Sbjct: 186 FKGKVPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDLSRN 245

Query: 256 LLEFNMSKLVFSTSLFTLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPLGGSLQ 315
           LLEFN+SK+VF  +L  LDLNHNKIFG +P  + KL  L   NVSYN LCG IP+GG LQ
Sbjct: 246 LLEFNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKL-ELQMLNVSYNALCGRIPMGGKLQ 305

Query: 316 RFDVYSYFHNKCLCGAPLASCK 338
            FDVYSYFHNKCLCG PL SCK
Sbjct: 306 SFDVYSYFHNKCLCGKPLGSCK 326

[Close]

BLAST of Tan0006718 vs. ExPASy TrEMBL
Match: A0A5D3BX47 (Polygalacturonase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold4559G00050 PE=4 SV=1)

HSP 1 Score: 471.1 bits (1211), Expect = 3.9e-129
Identity = 227/322 (70.50%), Postives = 254/322 (78.88%), Query Frame = 0


Query: 16  LLLLLFLFFTISFAERCDPNDKKALLNIKKAFNNPYVLASWKPEEDCCSWYCVDCDDNSD 75
           LLLLLF FFT+SFAE C PNDK+ LLNIKKAFNNPY+L SWKPEEDCC+WYCV+CD  S 
Sbjct: 6   LLLLLFFFFTVSFAELCHPNDKEVLLNIKKAFNNPYILTSWKPEEDCCTWYCVECDLKSH 65

Query: 76  RIISLNIITDDELSGQIPPSVGDLPFLQYLTFHKLPNLVGPIQPTIAKLHNLKFLDISWT 135
           RII+L I  DDELSG IPP VGDLPFL+ L FHKLPNL GPI PTIAKLHNLK+LD+SW 
Sbjct: 66  RIIALTIFADDELSGPIPPFVGDLPFLENLMFHKLPNLTGPIPPTIAKLHNLKYLDLSWN 125

Query: 136 NLSGSIPDFLSTLSHLSYIRLSFNNLTGTIPGSLSKLPKLESLQLDRNKLTGPIPDSFGY 195
            LSG IP FL +LS+L  + LSFN  TG+IP SL+ L +L +L LDRNKLTGPIPDSFG 
Sbjct: 126 GLSGPIPSFLGSLSNLDILDLSFNRFTGSIPSSLANLRRLGTLHLDRNKLTGPIPDSFGN 185

Query: 196 LKAKSLYLYLSHNKLSGKIPPSLSKVDFSAIDFSRNKLQGDASMLFGPNKKAWKVDLSRN 255
            K K  YLYLSHN+LSGKIP S+ KVDF+ ID SRNKL GD S++FG  K    VDLSRN
Sbjct: 186 FKGKVPYLYLSHNQLSGKIPTSMGKVDFNYIDLSRNKLVGDGSLIFGSKKTTEIVDLSRN 245

Query: 256 LLEFNMSKLVFSTSLFTLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPLGGSLQ 315
           LLEFN+SK+VF  +L  LDLNHNKIFG +P  + KL  L   NVSYN LCG IP+GG LQ
Sbjct: 246 LLEFNISKVVFPRTLTWLDLNHNKIFGEIPTGVVKL-ELQMLNVSYNALCGRIPMGGKLQ 305

Query: 316 RFDVYSYFHNKCLCGAPLASCK 338
            FDVYSYFHNKCLCG PL SCK
Sbjct: 306 SFDVYSYFHNKCLCGKPLGSCK 326

[Close]

BLAST of Tan0006718 vs. TAIR 10
Match: AT5G06870.1 (polygalacturonase inhibiting protein 2 )

HSP 1 Score: 390.2 bits (1001), Expect = 1.7e-108
Identity = 196/327 (59.94%), Postives = 234/327 (71.56%), Query Frame = 0


Query: 13  TFFLLLLLFLFFTISFAERCDPNDKKALLNIKKAFNNPYVLASWKPEEDCCSWYCVDCDD 72
           T FLLL   L  T    + C  +DK  LL IKK+ NNPY LASW P+ DCCSWYC++C D
Sbjct: 6   TLFLLLSTLLLTTSLAKDLCHKDDKTTLLKIKKSLNNPYHLASWDPKTDCCSWYCLECGD 65

Query: 73  N--SDRIISLNIITDDELSGQIPPSVGDLPFLQYLTFHKLPNLVGPIQPTIAKLHNLKFL 132
              + R+ SL II D E+SGQIPP VGDLP+L  L F KL NL G IQPTIAKL NL FL
Sbjct: 66  ATVNHRVTSL-IIQDGEISGQIPPEVGDLPYLTSLIFRKLTNLTGHIQPTIAKLKNLTFL 125

Query: 133 DISWTNLSGSIPDFLSTLSHLSYIRLSFNNLTGTIPGSLSKLPKLESLQLDRNKLTGPIP 192
            +SWTNL+G +P+FLS L +L YI LSFN+L+G+IP SLS L KLE L+L RNKLTGPIP
Sbjct: 126 RLSWTNLTGPVPEFLSQLKNLEYIDLSFNDLSGSIPSSLSSLRKLEYLELSRNKLTGPIP 185

Query: 193 DSFGYLKAKSLYLYLSHNKLSGKIPPSLSKVDFSAIDFSRNKLQGDASMLFGPNKKAWKV 252
           +SFG    K   L+LSHN+LSG IP SL   DF  ID SRNKLQGDAS+LFG  K  W V
Sbjct: 186 ESFGTFSGKVPSLFLSHNQLSGTIPKSLGNPDFYRIDLSRNKLQGDASILFGAKKTTWIV 245

Query: 253 DLSRNLLEFNMSKLVFSTSLFTLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPL 312
           D+SRN+ +F++SK+  + +L  LD+NHN I G +P + +K +     NVSYNRLCG IP 
Sbjct: 246 DISRNMFQFDLSKVKLAKTLNNLDMNHNGITGSIPAEWSK-AYFQLLNVSYNRLCGRIPK 305

Query: 313 GGSLQRFDVYSYFHNKCLCGAPLASCK 338
           G  +QRFD YS+FHNKCLCGAPL SCK
Sbjct: 306 GEYIQRFDSYSFFHNKCLCGAPLPSCK 330

[Close]

BLAST of Tan0006718 vs. TAIR 10
Match: AT5G06860.1 (polygalacturonase inhibiting protein 1 )

HSP 1 Score: 389.8 bits (1000), Expect = 2.2e-108
Identity = 192/324 (59.26%), Postives = 235/324 (72.53%), Query Frame = 0


Query: 17  LLLLFLFFTISFA-ERCDPNDKKALLNIKKAFNNPYVLASWKPEEDCCSWYCVDCDDN-- 76
           LL LF F T   + + C+ NDK  LL IKK+ NNPY LASW P+ DCCSWYC++C D   
Sbjct: 9   LLFLFTFLTTCLSKDLCNQNDKNTLLKIKKSLNNPYHLASWDPQTDCCSWYCLECGDATV 68

Query: 77  SDRIISLNIITDDELSGQIPPSVGDLPFLQYLTFHKLPNLVGPIQPTIAKLHNLKFLDIS 136
           + R+ +L I +  ++SGQIP  VGDLP+L+ L F KL NL G IQPTIAKL NL+ L +S
Sbjct: 69  NHRVTALTIFS-GQISGQIPAEVGDLPYLETLVFRKLSNLTGTIQPTIAKLKNLRMLRLS 128

Query: 137 WTNLSGSIPDFLSTLSHLSYIRLSFNNLTGTIPGSLSKLPKLESLQLDRNKLTGPIPDSF 196
           WTNL+G IPDF+S L +L ++ LSFN+L+G+IP SLS LPK+ +L+L RNKLTG IP+SF
Sbjct: 129 WTNLTGPIPDFISQLKNLEFLELSFNDLSGSIPSSLSTLPKILALELSRNKLTGSIPESF 188

Query: 197 GYLKAKSLYLYLSHNKLSGKIPPSLSKVDFSAIDFSRNKLQGDASMLFGPNKKAWKVDLS 256
           G        L LSHN+LSG IP SL  +DF+ ID SRNKLQGDASMLFG NK  W +DLS
Sbjct: 189 GSFPGTVPDLRLSHNQLSGPIPKSLGNIDFNRIDLSRNKLQGDASMLFGSNKTTWSIDLS 248

Query: 257 RNLLEFNMSKLVFSTSLFTLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPLGGS 316
           RN+ +F++SK+    +L  LDLNHN I G +P Q T+ + L  FNVSYN+LCG IP GG 
Sbjct: 249 RNMFQFDISKVDIPKTLGILDLNHNGITGNIPVQWTE-APLQFFNVSYNKLCGHIPTGGK 308

Query: 317 LQRFDVYSYFHNKCLCGAPLASCK 338
           LQ FD YSYFHNKCLCGAPL  CK
Sbjct: 309 LQTFDSYSYFHNKCLCGAPLEICK 330

[Close]

BLAST of Tan0006718 vs. TAIR 10
Match: AT3G12145.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 319.3 bits (817), Expect = 3.7e-87
Identity = 165/316 (52.22%), Postives = 214/316 (67.72%), Query Frame = 0


Query: 22  LFFTISFAERCDPNDKKALLNIKKAFNNPYVLASWKPEEDCCS-WYCVDCDDNSDRIISL 81
           LF T+  +  C  NDK ALL IKKA  NP +L+SW P  DCC+ W  V+C +   R+  L
Sbjct: 14  LFITLPSSYSCTENDKNALLQIKKALGNPPLLSSWNPRTDCCTGWTGVECTNR--RVTGL 73

Query: 82  NIITDDELSGQIPPSVGDLPFLQYLTFHKLPNLVGPIQPTIAKLHNLKFLDISWTNLSGS 141
           + +T  E+SGQI   +GDL  L+ L F  LP+L G I  TI KL NL  L +  T+LSG 
Sbjct: 74  S-VTSGEVSGQISYQIGDLVDLRTLDFSYLPHLTGNIPRTITKLKNLNTLYLKHTSLSGP 133

Query: 142 IPDFLSTLSHLSYIRLSFNNLTGTIPGSLSKLPKLESLQLDRNKLTGPIPDSFGYLKAKS 201
           IPD++S L  L+++ LSFN  TG IPGSLS++PKLE++Q++ NKLTG IP+SFG      
Sbjct: 134 IPDYISELKSLTFLDLSFNQFTGPIPGSLSQMPKLEAIQINDNKLTGSIPNSFGSFVGNV 193

Query: 202 LYLYLSHNKLSGKIPPSLSKVDFSAIDFSRNKLQGDASMLFGPNKKAWKVDLSRNLLEFN 261
             LYLS+NKLSGKIP SLSK DF+A+D S N  +GDA M FG NK   +VDLSRN+  F+
Sbjct: 194 PNLYLSNNKLSGKIPESLSKYDFNAVDLSGNGFEGDAFMFFGRNKTTVRVDLSRNMFNFD 253

Query: 262 MSKLVFSTSLFTLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPLGGSLQRFDVY 321
           + K+ F+ S+ +LDL+ N I+G +PP +TKL +L  FNVS N LCG IP GG LQ F+  
Sbjct: 254 LVKVKFARSIVSLDLSQNHIYGKIPPALTKL-HLEHFNVSDNHLCGKIPSGGLLQTFEPS 313

Query: 322 SYFHNKCLCGAPLASC 337
           ++ HN CLCG PL +C
Sbjct: 314 AFAHNICLCGTPLKAC 325

[Close]

BLAST of Tan0006718 vs. TAIR 10
Match: AT3G12610.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 186.8 bits (473), Expect = 2.8e-47
Identity = 118/365 (32.33%), Postives = 180/365 (49.32%), Query Frame = 0


Query: 12  STFFLLLLLFLFFTISFAERCDPNDKKALLNIKKAFNNPY--VLASWKPEEDCC-SWYCV 71
           S F  LL +     IS    C P D+ AL   K + + P   +  +W    DCC  WY +
Sbjct: 7   SPFSSLLAVVFISVISVVRCCSPKDQTALNAFKSSLSEPNLGIFNTWSENTDCCKEWYGI 66

Query: 72  DCDDNSDRIISLNIITDDE------------LSGQIPPSVGDLPFLQYLTFHKLPNLVGP 131
            CD +S R+  +++  + E            +SG I P+V DL  L  L       + G 
Sbjct: 67  SCDPDSGRVTDISLRGESEDAIFQKAGRSGYMSGSIDPAVCDLTALTSLVLADWKGITGE 126

Query: 132 IQPTIAKLHNLKFLDISWTNLSGSIPDFLSTLSHLSYIRLSFNNLTGTIPGSLSKLPKLE 191
           I P I  L +L+ LD++   ++G IP  +  LS L+ + L+ N ++G IP SL+ L +L+
Sbjct: 127 IPPCITSLASLRILDLAGNKITGEIPAEIGKLSKLAVLNLAENQMSGEIPASLTSLIELK 186

Query: 192 SLQLDRNKLTGPIPDSFGYLKAKSLYLYLSHNKLSGKIPPSLSKVD-FSAIDFSRNKLQG 251
            L+L  N +TG IP  FG LK  S  L L  N+L+G IP S+S ++  + +D S+N ++G
Sbjct: 187 HLELTENGITGVIPADFGSLKMLSRVL-LGRNELTGSIPESISGMERLADLDLSKNHIEG 246

Query: 252 -----------------DASMLFGP-------NKKAWKVDLSRNLLEFNMSKLVFS-TSL 311
                            D + L GP       N      +LSRN LE  +  +  S T L
Sbjct: 247 PIPEWMGNMKVLSLLNLDCNSLTGPIPGSLLSNSGLDVANLSRNALEGTIPDVFGSKTYL 306

Query: 312 FTLDLNHNKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPLGGSLQRFDVYSYFHNKCLCG 336
            +LDL+HN + G +P  ++    +   ++S+N+LCG IP G      +  S+  N+CLCG
Sbjct: 307 VSLDLSHNSLSGRIPDSLSSAKFVGHLDISHNKLCGRIPTGFPFDHLEATSFSDNQCLCG 366

[Close]

BLAST of Tan0006718 vs. TAIR 10
Match: AT3G20820.1 (Leucine-rich repeat (LRR) family protein )

HSP 1 Score: 175.6 bits (444), Expect = 6.6e-44
Identity = 110/359 (30.64%), Postives = 180/359 (50.14%), Query Frame = 0


Query: 17  LLLLFLFFTISFAERCDPNDKKALLNIKKAFNNPY--VLASWKPEEDCCSWYCVDCDDNS 76
           +++L L   IS A  C P+D++ALL  + A + PY  +  SW  ++ C +WY + CD  +
Sbjct: 5   VVVLLLLLLISSATCCPPSDRRALLAFRSALHEPYLGIFNSWTGQDCCHNWYGISCDSLT 64

Query: 77  DRIISLNIITDDE------------LSGQIPPSVGDLPFLQYLTFHKLPNLVGPIQPTIA 136
            R+  +N+  + E            ++G I  S+ +L  L  +T      + G I   I 
Sbjct: 65  HRVADINLRGESEDPIFERAHRTGYMTGHISASICELTRLSAITIADWKGISGEIPKCIT 124

Query: 137 KLHNLKFLDISWTNLSGSIPDFLSTLSHLSYIRLSFNNLTGTIPGSLSKLPKLESLQLDR 196
           +L  L+ LD+    +SG IP  +  L+ L+ + ++ N ++G+IP SL+ L  L  L L  
Sbjct: 125 RLPFLRTLDLIGNQISGGIPYDIGRLNRLAVLNVADNRISGSIPKSLTNLSSLMHLDLRN 184

Query: 197 NKLTGPIPDSFGYLKAKSLYLYLSHNKLSGKIPPSLSKV-DFSAIDFSRNKLQG------ 256
           N ++G IP   G LK  S  L LS N+++G+IP SL+ +   + +D S N+L G      
Sbjct: 185 NLISGVIPSDVGRLKMLSRAL-LSGNRITGRIPESLTNIYRLADVDLSGNQLYGTIPPSL 244

Query: 257 -----------DASMLFGP------NKKAWKVDLSRNLLEFNMSKLVFSTSLFT-LDLNH 316
                      D + + G             ++LSRNLL+  + +     S FT LDL++
Sbjct: 245 GRMSVLATLNLDGNKISGEIPQTLMTSSVMNLNLSRNLLQGKIPEGFGPRSYFTVLDLSY 304

Query: 317 NKIFGGLPPQMTKLSNLNSFNVSYNRLCGPIPLGGSLQRFDVYSYFHNKCLCGAPLASC 337
           N + G +P  ++  S +   ++S+N LCG IP+G      +  S+  N CLCG PL +C
Sbjct: 305 NNLKGPIPRSISGASFIGHLDLSHNHLCGRIPVGSPFDHLEAASFMFNDCLCGKPLRAC 362

The following BLAST results are available for this feature:

BLAST of Tan0006718 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q05091	4.8e-116	63.30	Polygalacturonase inhibitor OS=Pyrus communis OX=23211 GN=PGIP PE=1 SV=1	[more]
Q9M5J8	2.4e-107	59.94	Polygalacturonase inhibitor 2 OS=Arabidopsis thaliana OX=3702 GN=PGIP2 PE=2 SV=2	[more]
Q9M5J9	3.1e-107	59.26	Polygalacturonase inhibitor 1 OS=Arabidopsis thaliana OX=3702 GN=PGIP1 PE=1 SV=1	[more]
A7PW81	1.5e-106	59.82	Polygalacturonase inhibitor OS=Vitis vinifera OX=29760 GN=pgip PE=1 SV=1	[more]
Q9LH52	5.2e-86	52.22	Leucine-rich repeat protein FLOR 1 OS=Arabidopsis thaliana OX=3702 GN=FLR1 PE=1 ...	[more]

BLAST of Tan0006718 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023517556.1	4.3e-154	79.82	polygalacturonase inhibitor-like [Cucurbita pepo subsp. pepo]	[more]
KAG6595655.1	4.7e-153	79.23	Polygalacturonase inhibitor, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022966387.1	6.2e-153	79.23	polygalacturonase inhibitor-like [Cucurbita maxima]	[more]
XP_022925061.1	1.8e-152	78.64	polygalacturonase inhibitor-like [Cucurbita moschata]	[more]
KAG6595656.1	6.2e-145	76.85	Polygalacturonase inhibitor, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Tan0006718 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HMU3	3.0e-153	79.23	polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111466050 PE=...	[more]
A0A6J1EB58	8.7e-153	78.64	polygalacturonase inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111432422 P...	[more]
A0A6J1HP52	1.9e-144	76.56	polygalacturonase inhibitor-like OS=Cucurbita maxima OX=3661 GN=LOC111466048 PE=...	[more]
Q7X9Q7	3.9e-129	70.50	Polygalacturonase-inhibiting protein OS=Cucumis melo OX=3656 GN=LOC103499550 PE=...	[more]
A0A5D3BX47	3.9e-129	70.50	Polygalacturonase inhibitor-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676...	[more]

BLAST of Tan0006718 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G06870.1	1.7e-108	59.94	polygalacturonase inhibiting protein 2	[more]
AT5G06860.1	2.2e-108	59.26	polygalacturonase inhibiting protein 1	[more]
AT3G12145.1	3.7e-87	52.22	Leucine-rich repeat (LRR) family protein	[more]
AT3G12610.1	2.8e-47	32.33	Leucine-rich repeat (LRR) family protein	[more]
AT3G20820.1	6.6e-44	30.64	Leucine-rich repeat (LRR) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001611	Leucine-rich repeat	PFAM	PF00560	LRR_1	coord: 202..220 e-value: 0.67 score: 10.7
IPR001611	Leucine-rich repeat	PFAM	PF13855	LRR_8	coord: 126..185 e-value: 1.4E-8 score: 34.4
IPR013210	Leucine-rich repeat-containing N-terminal, plant-type	PFAM	PF08263	LRRNT_2	coord: 34..71 e-value: 2.4E-11 score: 43.7
IPR032675	Leucine-rich repeat domain superfamily	GENE3D	3.80.10.10	Ribonuclease Inhibitor	coord: 31..337 e-value: 2.2E-84 score: 285.2
None	No IPR available	PANTHER	PTHR48059:SF10	POLYGALACTURONASE INHIBITOR 2	coord: 14..336
None	No IPR available	PANTHER	PTHR48059	POLYGALACTURONASE INHIBITOR 1	coord: 14..336
None	No IPR available	SUPERFAMILY	52058	L domain-like	coord: 31..335

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0006718.1	Tan0006718.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0090353	polygalacturonase inhibitor activity
molecular_function	GO:0005515	protein binding