Tan0006694 (gene) Snake gourd v1

Overview


Name	Tan0006694
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Plant invertase/pectin methylesterase inhibitor superfamily protein
Location	LG01: 3215718 .. 3216829 (+)
RNA-Seq Expression	Tan0006694
Synteny	Tan0006694

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGTCCCTTCCTTAAGAGCTTTCTTGTGTATGAATGCATGGGACCCATGAAAATTAAACCCTTTAGACCTTTTCCATTGGCTTATAAATACAAAACCCATTTCCCTCTCTCTCATTCATCCCCCATTAATTAGAAAAAAATCATCAACCAAAACCAAAAAGAAGAAAAAAAAAGAAATGGGTCTCTCAATTTTCAAGCAAAGTTTGATTCTTTTTGTAGTAATCTTCCAATTAACAGTACTAAACCCAATGTTCACACATTCAGCCATCACTCCACACAGCAGCACTGAGTTCATCAAAAGCTCATGCAGCTCCACTGCTTACCCTCGTCTCTGTTTCTCATCCCTCTCAGTTCACTCCAGTTCCATCCAAACAAGCCCTCGCCTTCTCGCCACCGCCGCCCTCTCCGTCTCCCTCTCCTCCGTCAAGTCCGCCACCGCCCAAATCCTCAAGCTCTCCCACACTCATGGCCTCCCTCCCCGAGACGTCTCCGCCCTTAACGACTGTCTGGAAGAGCTCGGCGACTCTGTCGACTCCCTCGCAAGCTCCATTTCTGAAATGCCAAAGCTCCGAGGCCACGATTTCGAGCTCGTTATGAGCGATATCCAGACGTGGGTCAGCGCTGCCCTCACTGATGAGACCACTTGTAGCGACGGGTTTCAAGGGAAGGCGGTGAACGGCGGCGTTAAGGCGGCGGTGAGGACGAAGATTGTGAACATTGCTCAGTTGACTAGTAATGCTCTGTCTTTGATCAACAGGATTGGTGAGCTGCATTAGATTGTTTAATGGTGGATTTTGTTGGTAGGGATGTGTACTATAATAATACTGTCACTGTGTGAGATTTGAAAGATGTAATTGTAAAGTATAAAAGTTAGTTAAAAGAGGGTGGAGTTTTATACCATATTATCCATACCTAGAAATAGGGTTTTAATTAGGGTTTGTGTGGAGGTTGGAGTTATTTTTGCATTATTGCCATGTAGTCTGTATTATTGAGTTTTGGATTTATTAAAGTTAATTAAAGTTTGTGTGAGAGAATTTTGAAAACTCCTACTTTTTTATGTGACTATTTCCGTTGATATTTTAAGTCCCCGTTTGATATTCCTTTGGTTTTTGA

mRNA sequence

TGTCCCTTCCTTAAGAGCTTTCTTGTGTATGAATGCATGGGACCCATGAAAATTAAACCCTTTAGACCTTTTCCATTGGCTTATAAATACAAAACCCATTTCCCTCTCTCTCATTCATCCCCCATTAATTAGAAAAAAATCATCAACCAAAACCAAAAAGAAGAAAAAAAAAGAAATGGGTCTCTCAATTTTCAAGCAAAGTTTGATTCTTTTTGTAGTAATCTTCCAATTAACAGTACTAAACCCAATGTTCACACATTCAGCCATCACTCCACACAGCAGCACTGAGTTCATCAAAAGCTCATGCAGCTCCACTGCTTACCCTCGTCTCTGTTTCTCATCCCTCTCAGTTCACTCCAGTTCCATCCAAACAAGCCCTCGCCTTCTCGCCACCGCCGCCCTCTCCGTCTCCCTCTCCTCCGTCAAGTCCGCCACCGCCCAAATCCTCAAGCTCTCCCACACTCATGGCCTCCCTCCCCGAGACGTCTCCGCCCTTAACGACTGTCTGGAAGAGCTCGGCGACTCTGTCGACTCCCTCGCAAGCTCCATTTCTGAAATGCCAAAGCTCCGAGGCCACGATTTCGAGCTCGTTATGAGCGATATCCAGACGTGGGTCAGCGCTGCCCTCACTGATGAGACCACTTGTAGCGACGGGTTTCAAGGGAAGGCGGTGAACGGCGGCGTTAAGGCGGCGGTGAGGACGAAGATTGTGAACATTGCTCAGTTGACTAGTAATGCTCTGTCTTTGATCAACAGGATTGGTGAGCTGCATTAGATTGTTTAATGGTGGATTTTGTTGGTAGGGATGTGTACTATAATAATACTGTCACTGTGTGAGATTTGAAAGATGTAATTGTAAAGTATAAAAGTTAGTTAAAAGAGGGTGGAGTTTTATACCATATTATCCATACCTAGAAATAGGGTTTTAATTAGGGTTTGTGTGGAGGTTGGAGTTATTTTTGCATTATTGCCATGTAGTCTGTATTATTGAGTTTTGGATTTATTAAAGTTAATTAAAGTTTGTGTGAGAGAATTTTGAAAACTCCTACTTTTTTATGTGACTATTTCCGTTGATATTTTAAGTCCCCGTTTGATATTCCTTTGGTTTTTGA

Coding sequence (CDS)

ATGGGTCTCTCAATTTTCAAGCAAAGTTTGATTCTTTTTGTAGTAATCTTCCAATTAACAGTACTAAACCCAATGTTCACACATTCAGCCATCACTCCACACAGCAGCACTGAGTTCATCAAAAGCTCATGCAGCTCCACTGCTTACCCTCGTCTCTGTTTCTCATCCCTCTCAGTTCACTCCAGTTCCATCCAAACAAGCCCTCGCCTTCTCGCCACCGCCGCCCTCTCCGTCTCCCTCTCCTCCGTCAAGTCCGCCACCGCCCAAATCCTCAAGCTCTCCCACACTCATGGCCTCCCTCCCCGAGACGTCTCCGCCCTTAACGACTGTCTGGAAGAGCTCGGCGACTCTGTCGACTCCCTCGCAAGCTCCATTTCTGAAATGCCAAAGCTCCGAGGCCACGATTTCGAGCTCGTTATGAGCGATATCCAGACGTGGGTCAGCGCTGCCCTCACTGATGAGACCACTTGTAGCGACGGGTTTCAAGGGAAGGCGGTGAACGGCGGCGTTAAGGCGGCGGTGAGGACGAAGATTGTGAACATTGCTCAGTTGACTAGTAATGCTCTGTCTTTGATCAACAGGATTGGTGAGCTGCATTAG

Protein sequence

MGLSIFKQSLILFVVIFQLTVLNPMFTHSAITPHSSTEFIKSSCSSTAYPRLCFSSLSVHSSSIQTSPRLLATAALSVSLSSVKSATAQILKLSHTHGLPPRDVSALNDCLEELGDSVDSLASSISEMPKLRGHDFELVMSDIQTWVSAALTDETTCSDGFQGKAVNGGVKAAVRTKIVNIAQLTSNALSLINRIGELH

Homology

[Close]

BLAST of Tan0006694 vs. ExPASy Swiss-Prot
Match: Q9SB37 (Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 3.8e-36
Identity = 90/198 (45.45%), Postives = 127/198 (64.14%), Query Frame = 0


Query: 6   FKQSLILFVVIFQLTVLNPMFTHSAITPHSSTEFIKSSCSSTAYPRLCFSSLSVHSSSIQ 65
           F+ SLILF V++  T    M  +        TEFIK+SC +T+YP  CF SLS ++S I+
Sbjct: 5   FELSLILF-VLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIK 64

Query: 66  TSPRLLATAALSVSLSSVKSATAQILKLSHTHGLPPRDVSALNDCLEELGDSVDSLASSI 125
             PR LA  AL+VS++  KSA   + +++   G+  R   A+ DCLEE+GD+VD L++S+
Sbjct: 65  KQPRKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSL 124

Query: 126 SEMPKLR----GHDFELVMSDIQTWVSAALTDETTCSDGFQGKAVNGGVKAAVRTKIVNI 185
            E+  L     G DF   +S+++TW SAALTDET C DGF GKA+ G +K+ +RT IV++
Sbjct: 125 KELKHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSV 184

Query: 186 AQLTSNALSLINRIGELH 200
           A+ TSNAL+LIN     H
Sbjct: 185 AEETSNALALINDFASKH 201

[Close]

BLAST of Tan0006694 vs. ExPASy Swiss-Prot
Match: P17407 (21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 1.6e-34
Identity = 78/174 (44.83%), Postives = 119/174 (68.39%), Query Frame = 0


Query: 29  SAITPHSSTEFIKSSCSSTAYPRLCFSSLSVHSSSIQTSPRLLATAALSVSLSSVKSATA 88
           +A +   + +FIK+SC+ T YP +C  SLS ++ +IQ +P+ LA+ AL VSL+  + A  
Sbjct: 18  AAASAAPANQFIKTSCTLTTYPAVCEQSLSAYAKTIQNNPQELASTALQVSLTRTQQAQT 77

Query: 89  QILKLSHTHGLPPRDVSALNDCLEELGDSVDSLASSISEMPKL---RGHDFELVMSDIQT 148
            + +L+   GL  R  +A++DCLEE+ DS+D ++ S  EM  L   +G+DF   MS+++T
Sbjct: 78  FMKRLNKFKGLKARQYAAIHDCLEEVEDSLDRVSRSCDEMKNLSHAKGNDFTFRMSNVET 137

Query: 149 WVSAALTDETTCSDGFQGKAVNGGVKAAVRTKIVNIAQLTSNALSLINRIGELH 200
           WVSAALTDETTC DGF GK ++G +K +VR ++V +A++TSNAL+L+N     H
Sbjct: 138 WVSAALTDETTCMDGFAGKGMDGKIKESVRAQVVAVARVTSNALALVNNFAAKH 191

[Close]

BLAST of Tan0006694 vs. ExPASy Swiss-Prot
Match: Q9SI74 (Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 6.7e-33
Identity = 82/178 (46.07%), Postives = 123/178 (69.10%), Query Frame = 0


Query: 19  LTVLNPMFTHSAITPHSSTEFIKSSCSSTAYPRLCFSSLSVHSSSIQTSPRLLATAALSV 78
           L+ L+P  + S  +  ++ ++IK+SC+ T Y  +C++SLS ++S+I+++P+ LA  AL++
Sbjct: 127 LSSLSPSSSSSTYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNL 186

Query: 79  SLSSVKSATAQILKLSHTHGLPPRDVSALNDCLEELGDSVDSLASSISEMPKLRGHD--- 138
           +LSS KSA+  +  +SH  GL   +V A+ DC+EE+GDSV SL  SI E+  +   D   
Sbjct: 187 TLSSAKSASKFVKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAK 246

Query: 139 FELVMSDIQTWVSAALTDETTCSDGFQGKAVNGGVKAAVRTKIVNIAQLTSNALSLIN 194
           FE+VMSD++TWVSAALT++ TC DGF    V   VK  VR  +V +A+LTSNAL+LIN
Sbjct: 247 FEMVMSDVETWVSAALTNDDTCMDGF--SLVKTAVKDLVRRHVVEVARLTSNALALIN 302

[Close]

BLAST of Tan0006694 vs. ExPASy Swiss-Prot
Match: Q9SI72 (Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1)

HSP 1 Score: 136.3 bits (342), Expect = 3.7e-31
Identity = 85/198 (42.93%), Postives = 126/198 (63.64%), Query Frame = 0


Query: 8   QSLILFVVIFQLTVLNPMFTHSAITPH--SSTEFIKSSCSSTAYPRLCFSSLSVHSSSIQ 67
           ++ I  V++  +T+L      S+ TP+   S +FI SSC +T YP LC  +LS +++ I+
Sbjct: 4   KNTIFLVILLSITILQ----SSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIR 63

Query: 68  -TSPRLLATAALSVSLSSVKSATAQILKLS-HTHGLPPRDVSALNDCLEELGDSVDSLAS 127
             + + LA  AL +SL+  KS T  + KL+  T     R+  A+ DC+E LG+SVD LA 
Sbjct: 64  HNNDQDLAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQ 123

Query: 128 SISEMPK----LRGHDFELVMSDIQTWVSAALTDETTCSDGFQGKAVNGGVKAAVRTKIV 187
           S+ E+ +    +   DF   MS++QTWVSAALTDETTC DGF  +A+ G VK  +R K+V
Sbjct: 124 SVKELARAGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVV 183

Query: 188 NIAQLTSNALSLINRIGE 198
           ++AQ+TSNAL+L+N+  E
Sbjct: 184 HVAQVTSNALALVNQFAE 197

[Close]

BLAST of Tan0006694 vs. ExPASy Swiss-Prot
Match: Q9STY5 (Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 1.8e-30
Identity = 79/189 (41.80%), Postives = 121/189 (64.02%), Query Frame = 0


Query: 9   SLILFVVIFQLTVLNPMFTHSAITPHSSTEFIKSSCSSTAYPRLCFSSLSVHSSSIQTSP 68
           +L LF++   +   +     +AIT   +  FI++SC +T YP +C +SL+ +++SIQTSP
Sbjct: 8   TLFLFLLSTAILTASSSAPRAAITSKRAINFIQASCKATTYPTVCVNSLTGYANSIQTSP 67

Query: 69  RLLATAALSVSLSSVKSATAQILKLSHTHGLPPRDVSALNDCLEELGDSVDSLASSISEM 128
           R LA  AL+V+++  +S    + +L     L  R++ A+ DC+EE+ D+VD L  SI E+
Sbjct: 68  RRLAETALNVTVTQAQSTKVFVWRLGRFTSLKKREIQAVKDCIEEIHDAVDRLTMSIHEV 127

Query: 129 P---KLRGHD-FELVMSDIQTWVSAALTDETTCSDGFQGKAVNGGVKAAVRTKIVNIAQL 188
                 +G D F   MS+ QTW SAALT+  TCSDGF G+ ++G VK +VR +I+N+ + 
Sbjct: 128 KMCGSAKGRDQFWFHMSNAQTWTSAALTNANTCSDGFAGRVMDGRVKNSVRARILNLGRG 187

Query: 189 TSNALSLIN 194
           TSNAL+LIN
Sbjct: 188 TSNALALIN 196

[Close]

BLAST of Tan0006694 vs. NCBI nr
Match: XP_023551428.1 (21 kDa protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 312.8 bits (800), Expect = 2.2e-81
Identity = 172/200 (86.00%), Postives = 186/200 (93.00%), Query Frame = 0


Query: 1   MGLSIFKQSLILFVVIFQLTVLNPMFTHSAITPH-SSTEFIKSSCSSTAYPRLCFSSLSV 60
           MG SI KQSLIL V IFQLT LNP+F +SAITP  SSTEFIK+SCSST YPRLCFSSLS+
Sbjct: 1   MGFSICKQSLILLVAIFQLT-LNPIFIYSAITPQSSSTEFIKTSCSSTTYPRLCFSSLSL 60

Query: 61  HSSSIQTSPRLLATAALSVSLSSVKSATAQILKLSHTHGLPPRDVSALNDCLEELGDSVD 120
           H++SIQTSPRLLATAALSVS+SSVKSAT QILKLSH HGL PRDVSALNDCLEELGDSVD
Sbjct: 61  HATSIQTSPRLLATAALSVSISSVKSATTQILKLSHAHGLSPRDVSALNDCLEELGDSVD 120

Query: 121 SLASSISEMPKLRGHDFELVMSDIQTWVSAALTDETTCSDGFQGKAVNGGVKAAVRTKIV 180
           SL SSISEMPKLRG+DF++VMS+IQTWVSAALTDETTCS+GFQGKAVNGGVKAAVR+KIV
Sbjct: 121 SLISSISEMPKLRGNDFDVVMSNIQTWVSAALTDETTCSEGFQGKAVNGGVKAAVRSKIV 180

Query: 181 NIAQLTSNALSLINRIGELH 200
           NIAQLTSNALSLINR+ ELH
Sbjct: 181 NIAQLTSNALSLINRMAELH 199

[Close]

BLAST of Tan0006694 vs. NCBI nr
Match: XP_022943093.1 (21 kDa protein-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 312.4 bits (799), Expect = 2.8e-81
Identity = 172/200 (86.00%), Postives = 184/200 (92.00%), Query Frame = 0


Query: 1   MGLSIFKQSLILFVVIFQLTVLNPMFTHSAITPH-SSTEFIKSSCSSTAYPRLCFSSLSV 60
           MG SI KQSLIL V IFQLT LNP+F HSAITP  SSTEFIK+SCSST YPRLCFSSLS+
Sbjct: 1   MGFSICKQSLILLVAIFQLT-LNPIFIHSAITPQSSSTEFIKTSCSSTTYPRLCFSSLSL 60

Query: 61  HSSSIQTSPRLLATAALSVSLSSVKSATAQILKLSHTHGLPPRDVSALNDCLEELGDSVD 120
           H++SIQTSPRLLATAALSVS+SSVKSAT QILKLSH HGL PRDVSALNDCLEELGDSVD
Sbjct: 61  HAASIQTSPRLLATAALSVSISSVKSATTQILKLSHAHGLSPRDVSALNDCLEELGDSVD 120

Query: 121 SLASSISEMPKLRGHDFELVMSDIQTWVSAALTDETTCSDGFQGKAVNGGVKAAVRTKIV 180
           SL SSISEMPKLRG+DF++VMS+IQTWVSAALTDETTCS+GFQGKAVN GVKA VR+KIV
Sbjct: 121 SLISSISEMPKLRGNDFDVVMSNIQTWVSAALTDETTCSEGFQGKAVNSGVKAVVRSKIV 180

Query: 181 NIAQLTSNALSLINRIGELH 200
           NIAQLTSNALSLINRI ELH
Sbjct: 181 NIAQLTSNALSLINRIAELH 199

[Close]

BLAST of Tan0006694 vs. NCBI nr
Match: KAG6600168.1 (hypothetical protein SDJN03_05401, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 312.0 bits (798), Expect = 3.7e-81
Identity = 172/200 (86.00%), Postives = 185/200 (92.50%), Query Frame = 0


Query: 1   MGLSIFKQSLILFVVIFQLTVLNPMFTHSAITPH-SSTEFIKSSCSSTAYPRLCFSSLSV 60
           MG+SI KQSLIL V IFQLT LNP+F HSAITP  SSTEFIK+SCSST YPRLCFSSLS+
Sbjct: 1   MGVSICKQSLILLVAIFQLT-LNPIFIHSAITPQSSSTEFIKTSCSSTTYPRLCFSSLSL 60

Query: 61  HSSSIQTSPRLLATAALSVSLSSVKSATAQILKLSHTHGLPPRDVSALNDCLEELGDSVD 120
           H++SIQTSPRLLATAALSVS+SSVKSAT QILKLSH HGL PRDVSALNDCLEELGDSVD
Sbjct: 61  HAASIQTSPRLLATAALSVSISSVKSATTQILKLSHAHGLSPRDVSALNDCLEELGDSVD 120

Query: 121 SLASSISEMPKLRGHDFELVMSDIQTWVSAALTDETTCSDGFQGKAVNGGVKAAVRTKIV 180
           SL SSISEMPKLRG+DF++VMS+IQTWVSAALTDETTCS+GFQGKA N GVKAAVR+KIV
Sbjct: 121 SLISSISEMPKLRGNDFDVVMSNIQTWVSAALTDETTCSEGFQGKAENSGVKAAVRSKIV 180

Query: 181 NIAQLTSNALSLINRIGELH 200
           NIAQLTSNALSLINRI ELH
Sbjct: 181 NIAQLTSNALSLINRIAELH 199

[Close]

BLAST of Tan0006694 vs. NCBI nr
Match: XP_022989139.1 (21 kDa protein-like [Cucurbita maxima])

HSP 1 Score: 312.0 bits (798), Expect = 3.7e-81
Identity = 170/203 (83.74%), Postives = 185/203 (91.13%), Query Frame = 0


Query: 1   MGLSIFKQSLILFVVIFQLTVLNPMFTHSAITPH----SSTEFIKSSCSSTAYPRLCFSS 60
           MG SI KQSLIL V IFQLT LNP+F HSAITP     SSTEFIK+SCSST YPRLCFSS
Sbjct: 1   MGFSICKQSLILLVAIFQLT-LNPIFIHSAITPQSSSSSSTEFIKTSCSSTTYPRLCFSS 60

Query: 61  LSVHSSSIQTSPRLLATAALSVSLSSVKSATAQILKLSHTHGLPPRDVSALNDCLEELGD 120
           LS+H++SIQTSPRLLATAALSVS+SSVKSAT QILKLSH HGL PRDVSALNDCLEELGD
Sbjct: 61  LSLHATSIQTSPRLLATAALSVSISSVKSATTQILKLSHAHGLSPRDVSALNDCLEELGD 120

Query: 121 SVDSLASSISEMPKLRGHDFELVMSDIQTWVSAALTDETTCSDGFQGKAVNGGVKAAVRT 180
           S+DSL SSISEMPKLRG+DF++VMS+IQTWVSAALTDETTCS+GFQGK VNGG+KAAVR+
Sbjct: 121 SIDSLISSISEMPKLRGNDFDVVMSNIQTWVSAALTDETTCSEGFQGKTVNGGIKAAVRS 180

Query: 181 KIVNIAQLTSNALSLINRIGELH 200
           KIVNIAQLTSNA+SLINRI ELH
Sbjct: 181 KIVNIAQLTSNAISLINRIAELH 202

[Close]

BLAST of Tan0006694 vs. NCBI nr
Match: XP_022943092.1 (21 kDa protein-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 311.6 bits (797), Expect = 4.9e-81
Identity = 172/202 (85.15%), Postives = 184/202 (91.09%), Query Frame = 0


Query: 1   MGLSIFKQSLILFVVIFQLTVLNPMFTHSAITPH---SSTEFIKSSCSSTAYPRLCFSSL 60
           MG SI KQSLIL V IFQLT LNP+F HSAITP    SSTEFIK+SCSST YPRLCFSSL
Sbjct: 1   MGFSICKQSLILLVAIFQLT-LNPIFIHSAITPQSSSSSTEFIKTSCSSTTYPRLCFSSL 60

Query: 61  SVHSSSIQTSPRLLATAALSVSLSSVKSATAQILKLSHTHGLPPRDVSALNDCLEELGDS 120
           S+H++SIQTSPRLLATAALSVS+SSVKSAT QILKLSH HGL PRDVSALNDCLEELGDS
Sbjct: 61  SLHAASIQTSPRLLATAALSVSISSVKSATTQILKLSHAHGLSPRDVSALNDCLEELGDS 120

Query: 121 VDSLASSISEMPKLRGHDFELVMSDIQTWVSAALTDETTCSDGFQGKAVNGGVKAAVRTK 180
           VDSL SSISEMPKLRG+DF++VMS+IQTWVSAALTDETTCS+GFQGKAVN GVKA VR+K
Sbjct: 121 VDSLISSISEMPKLRGNDFDVVMSNIQTWVSAALTDETTCSEGFQGKAVNSGVKAVVRSK 180

Query: 181 IVNIAQLTSNALSLINRIGELH 200
           IVNIAQLTSNALSLINRI ELH
Sbjct: 181 IVNIAQLTSNALSLINRIAELH 201

[Close]

BLAST of Tan0006694 vs. ExPASy TrEMBL
Match: A0A6J1FWC1 (21 kDa protein-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111447928 PE=4 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 1.4e-81
Identity = 172/200 (86.00%), Postives = 184/200 (92.00%), Query Frame = 0


Query: 1   MGLSIFKQSLILFVVIFQLTVLNPMFTHSAITPH-SSTEFIKSSCSSTAYPRLCFSSLSV 60
           MG SI KQSLIL V IFQLT LNP+F HSAITP  SSTEFIK+SCSST YPRLCFSSLS+
Sbjct: 1   MGFSICKQSLILLVAIFQLT-LNPIFIHSAITPQSSSTEFIKTSCSSTTYPRLCFSSLSL 60

Query: 61  HSSSIQTSPRLLATAALSVSLSSVKSATAQILKLSHTHGLPPRDVSALNDCLEELGDSVD 120
           H++SIQTSPRLLATAALSVS+SSVKSAT QILKLSH HGL PRDVSALNDCLEELGDSVD
Sbjct: 61  HAASIQTSPRLLATAALSVSISSVKSATTQILKLSHAHGLSPRDVSALNDCLEELGDSVD 120

Query: 121 SLASSISEMPKLRGHDFELVMSDIQTWVSAALTDETTCSDGFQGKAVNGGVKAAVRTKIV 180
           SL SSISEMPKLRG+DF++VMS+IQTWVSAALTDETTCS+GFQGKAVN GVKA VR+KIV
Sbjct: 121 SLISSISEMPKLRGNDFDVVMSNIQTWVSAALTDETTCSEGFQGKAVNSGVKAVVRSKIV 180

Query: 181 NIAQLTSNALSLINRIGELH 200
           NIAQLTSNALSLINRI ELH
Sbjct: 181 NIAQLTSNALSLINRIAELH 199

[Close]

BLAST of Tan0006694 vs. ExPASy TrEMBL
Match: A0A6J1JNI8 (21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111486299 PE=4 SV=1)

HSP 1 Score: 312.0 bits (798), Expect = 1.8e-81
Identity = 170/203 (83.74%), Postives = 185/203 (91.13%), Query Frame = 0


Query: 1   MGLSIFKQSLILFVVIFQLTVLNPMFTHSAITPH----SSTEFIKSSCSSTAYPRLCFSS 60
           MG SI KQSLIL V IFQLT LNP+F HSAITP     SSTEFIK+SCSST YPRLCFSS
Sbjct: 1   MGFSICKQSLILLVAIFQLT-LNPIFIHSAITPQSSSSSSTEFIKTSCSSTTYPRLCFSS 60

Query: 61  LSVHSSSIQTSPRLLATAALSVSLSSVKSATAQILKLSHTHGLPPRDVSALNDCLEELGD 120
           LS+H++SIQTSPRLLATAALSVS+SSVKSAT QILKLSH HGL PRDVSALNDCLEELGD
Sbjct: 61  LSLHATSIQTSPRLLATAALSVSISSVKSATTQILKLSHAHGLSPRDVSALNDCLEELGD 120

Query: 121 SVDSLASSISEMPKLRGHDFELVMSDIQTWVSAALTDETTCSDGFQGKAVNGGVKAAVRT 180
           S+DSL SSISEMPKLRG+DF++VMS+IQTWVSAALTDETTCS+GFQGK VNGG+KAAVR+
Sbjct: 121 SIDSLISSISEMPKLRGNDFDVVMSNIQTWVSAALTDETTCSEGFQGKTVNGGIKAAVRS 180

Query: 181 KIVNIAQLTSNALSLINRIGELH 200
           KIVNIAQLTSNA+SLINRI ELH
Sbjct: 181 KIVNIAQLTSNAISLINRIAELH 202

[Close]

BLAST of Tan0006694 vs. ExPASy TrEMBL
Match: A0A6J1FS20 (21 kDa protein-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111447928 PE=4 SV=1)

HSP 1 Score: 311.6 bits (797), Expect = 2.3e-81
Identity = 172/202 (85.15%), Postives = 184/202 (91.09%), Query Frame = 0


Query: 1   MGLSIFKQSLILFVVIFQLTVLNPMFTHSAITPH---SSTEFIKSSCSSTAYPRLCFSSL 60
           MG SI KQSLIL V IFQLT LNP+F HSAITP    SSTEFIK+SCSST YPRLCFSSL
Sbjct: 1   MGFSICKQSLILLVAIFQLT-LNPIFIHSAITPQSSSSSTEFIKTSCSSTTYPRLCFSSL 60

Query: 61  SVHSSSIQTSPRLLATAALSVSLSSVKSATAQILKLSHTHGLPPRDVSALNDCLEELGDS 120
           S+H++SIQTSPRLLATAALSVS+SSVKSAT QILKLSH HGL PRDVSALNDCLEELGDS
Sbjct: 61  SLHAASIQTSPRLLATAALSVSISSVKSATTQILKLSHAHGLSPRDVSALNDCLEELGDS 120

Query: 121 VDSLASSISEMPKLRGHDFELVMSDIQTWVSAALTDETTCSDGFQGKAVNGGVKAAVRTK 180
           VDSL SSISEMPKLRG+DF++VMS+IQTWVSAALTDETTCS+GFQGKAVN GVKA VR+K
Sbjct: 121 VDSLISSISEMPKLRGNDFDVVMSNIQTWVSAALTDETTCSEGFQGKAVNSGVKAVVRSK 180

Query: 181 IVNIAQLTSNALSLINRIGELH 200
           IVNIAQLTSNALSLINRI ELH
Sbjct: 181 IVNIAQLTSNALSLINRIAELH 201

[Close]

BLAST of Tan0006694 vs. ExPASy TrEMBL
Match: A0A6J1FXD1 (pectinesterase inhibitor 7-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447928 PE=4 SV=1)

HSP 1 Score: 311.2 bits (796), Expect = 3.1e-81
Identity = 172/203 (84.73%), Postives = 184/203 (90.64%), Query Frame = 0


Query: 1   MGLSIFKQSLILFVVIFQLTVLNPMFTHSAITPH----SSTEFIKSSCSSTAYPRLCFSS 60
           MG SI KQSLIL V IFQLT LNP+F HSAITP     SSTEFIK+SCSST YPRLCFSS
Sbjct: 1   MGFSICKQSLILLVAIFQLT-LNPIFIHSAITPQSSSSSSTEFIKTSCSSTTYPRLCFSS 60

Query: 61  LSVHSSSIQTSPRLLATAALSVSLSSVKSATAQILKLSHTHGLPPRDVSALNDCLEELGD 120
           LS+H++SIQTSPRLLATAALSVS+SSVKSAT QILKLSH HGL PRDVSALNDCLEELGD
Sbjct: 61  LSLHAASIQTSPRLLATAALSVSISSVKSATTQILKLSHAHGLSPRDVSALNDCLEELGD 120

Query: 121 SVDSLASSISEMPKLRGHDFELVMSDIQTWVSAALTDETTCSDGFQGKAVNGGVKAAVRT 180
           SVDSL SSISEMPKLRG+DF++VMS+IQTWVSAALTDETTCS+GFQGKAVN GVKA VR+
Sbjct: 121 SVDSLISSISEMPKLRGNDFDVVMSNIQTWVSAALTDETTCSEGFQGKAVNSGVKAVVRS 180

Query: 181 KIVNIAQLTSNALSLINRIGELH 200
           KIVNIAQLTSNALSLINRI ELH
Sbjct: 181 KIVNIAQLTSNALSLINRIAELH 202

[Close]

BLAST of Tan0006694 vs. ExPASy TrEMBL
Match: A0A6J1E4J6 (21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111430716 PE=4 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 4.9e-79
Identity = 164/199 (82.41%), Postives = 178/199 (89.45%), Query Frame = 0


Query: 1   MGLSIFKQSLILFVVIFQLTVLNPMFTHSAITPHSSTEFIKSSCSSTAYPRLCFSSLSVH 60
           MGLSI K+SLI   +I QLT     F HSAITP SST+FI++SC+ST YPRLCFSSLS+H
Sbjct: 1   MGLSICKKSLIFVQIILQLT-----FIHSAITPQSSTQFIRTSCTSTKYPRLCFSSLSIH 60

Query: 61  SSSIQTSPRLLATAALSVSLSSVKSATAQILKLSHTHGLPPRDVSALNDCLEELGDSVDS 120
           ++SIQTSPRLLAT ALSV+LSSVKSA  QILKLSH HGLPPRDVSALNDCLEEL DSVDS
Sbjct: 61  ANSIQTSPRLLATTALSVTLSSVKSAATQILKLSHAHGLPPRDVSALNDCLEELSDSVDS 120

Query: 121 LASSISEMPKLRGHDFELVMSDIQTWVSAALTDETTCSDGFQGKAVNGGVKAAVRTKIVN 180
           LASSISEMPKLRGHDF+LVMSD+QTWVSAALTDETTCS+GFQGKAV GGVKAAVR +IVN
Sbjct: 121 LASSISEMPKLRGHDFDLVMSDVQTWVSAALTDETTCSEGFQGKAVKGGVKAAVRRRIVN 180

Query: 181 IAQLTSNALSLINRIGELH 200
           IAQLTSNALSLINRI ELH
Sbjct: 181 IAQLTSNALSLINRIAELH 194

[Close]

BLAST of Tan0006694 vs. TAIR 10
Match: AT5G62360.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 183.0 bits (463), Expect = 2.4e-46
Identity = 99/190 (52.11%), Postives = 141/190 (74.21%), Query Frame = 0


Query: 10  LILFVVIFQLTVLNPMFTHSAITPHSSTEFIKSSCSSTAYPRLCFSSLSVHSSSIQTSPR 69
           L  F++I  +  L    T +  T  ++TEF+KSSC+ T YPRLCFSSLS H+S IQTSP+
Sbjct: 14  LTTFLIIIAMLKLVHTTTTTTTTTTTNTEFVKSSCTFTTYPRLCFSSLSTHASLIQTSPK 73

Query: 70  LLATAALSVSLSSVKSATAQILKLSHTHGLPPRDVSALNDCLEELGDSVDSLASSISEMP 129
           L+A AAL+++L+S K  +A +++LS++  L P++VSA+ DC+EELGD+++ L  SI EM 
Sbjct: 74  LMAHAALNITLASAKVTSAMMVRLSNSR-LKPKEVSAMRDCVEELGDTLEELRKSIGEMC 133

Query: 130 KLRGHDFELVMSDIQTWVSAALTDETTCSDGFQGKAVNGGVKAAVRTKIVNIAQLTSNAL 189
           +L G ++E+ +SDIQTWVSAALTD  TC+DGF+G+ ++G VK  VR +I+ IA LTSNAL
Sbjct: 134 QLSGSNYEVYISDIQTWVSAALTDVNTCTDGFEGEDMDGKVKVLVRGRILVIAHLTSNAL 193

Query: 190 SLINRIGELH 200
           +LIN    +H
Sbjct: 194 ALINHFASIH 202

[Close]

BLAST of Tan0006694 vs. TAIR 10
Match: AT4G25260.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 152.9 bits (385), Expect = 2.7e-37
Identity = 90/198 (45.45%), Postives = 127/198 (64.14%), Query Frame = 0


Query: 6   FKQSLILFVVIFQLTVLNPMFTHSAITPHSSTEFIKSSCSSTAYPRLCFSSLSVHSSSIQ 65
           F+ SLILF V++  T    M  +        TEFIK+SC +T+YP  CF SLS ++S I+
Sbjct: 5   FELSLILF-VLYLSTAAIVMARNLEEESSGDTEFIKASCETTSYPDRCFQSLSSYASEIK 64

Query: 66  TSPRLLATAALSVSLSSVKSATAQILKLSHTHGLPPRDVSALNDCLEELGDSVDSLASSI 125
             PR LA  AL+VS++  KSA   + +++   G+  R   A+ DCLEE+GD+VD L++S+
Sbjct: 65  KQPRKLAETALAVSIARAKSAKTYVSEMTDYKGITKRQHEAVADCLEEMGDTVDRLSNSL 124

Query: 126 SEMPKLR----GHDFELVMSDIQTWVSAALTDETTCSDGFQGKAVNGGVKAAVRTKIVNI 185
            E+  L     G DF   +S+++TW SAALTDET C DGF GKA+ G +K+ +RT IV++
Sbjct: 125 KELKHLEEGDSGEDFWFCLSNVRTWTSAALTDETACMDGFGGKAMAGELKSLIRTHIVSV 184

Query: 186 AQLTSNALSLINRIGELH 200
           A+ TSNAL+LIN     H
Sbjct: 185 AEETSNALALINDFASKH 201

[Close]

BLAST of Tan0006694 vs. TAIR 10
Match: AT5G62350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 145.2 bits (365), Expect = 5.6e-35
Identity = 80/166 (48.19%), Postives = 111/166 (66.87%), Query Frame = 0


Query: 39  FIKSSCSSTAYPRLCFSSLSVHSSSIQTSPRLLATAALSVSLSSVKSATAQILKLSHTHG 98
           FI+SSC +T YP LC  SLSV+++ IQTSP+ LA  A++V+LS  +S    + +L+   G
Sbjct: 38  FIQSSCKTTTYPALCVHSLSVYANDIQTSPKRLAETAIAVTLSRAQSTKLFVSRLTRMKG 97

Query: 99  LPPRDVSALNDCLEELGDSVDSLASSISEMPKLRG-----HDFELVMSDIQTWVSAALTD 158
           L  R+V A+ DC+EE+ D+VD L  S+ E+ KL G       F   MS+ QTW SAALTD
Sbjct: 98  LKKREVEAIKDCVEEMNDTVDRLTKSVQEL-KLCGSAKDQDQFAYHMSNAQTWTSAALTD 157

Query: 159 ETTCSDGFQGKAVNGGVKAAVRTKIVNIAQLTSNALSLINRIGELH 200
           E TCSDGF G+ ++G +K +VR +I+N+   TSNALSLIN   + +
Sbjct: 158 ENTCSDGFSGRVMDGRIKNSVRARIMNVGHETSNALSLINAFAKTY 202

[Close]

BLAST of Tan0006694 vs. TAIR 10
Match: AT1G62760.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 142.1 bits (357), Expect = 4.7e-34
Identity = 82/178 (46.07%), Postives = 123/178 (69.10%), Query Frame = 0


Query: 19  LTVLNPMFTHSAITPHSSTEFIKSSCSSTAYPRLCFSSLSVHSSSIQTSPRLLATAALSV 78
           L+ L+P  + S  +  ++ ++IK+SC+ T Y  +C++SLS ++S+I+++P+ LA  AL++
Sbjct: 127 LSSLSPSSSSSTYSNQTNLDYIKTSCNITLYKTICYNSLSPYASTIRSNPQKLAVIALNL 186

Query: 79  SLSSVKSATAQILKLSHTHGLPPRDVSALNDCLEELGDSVDSLASSISEMPKLRGHD--- 138
           +LSS KSA+  +  +SH  GL   +V A+ DC+EE+GDSV SL  SI E+  +   D   
Sbjct: 187 TLSSAKSASKFVKNISHGGGLTRLEVVAVADCVEEIGDSVTSLQDSIRELDSINYKDSAK 246

Query: 139 FELVMSDIQTWVSAALTDETTCSDGFQGKAVNGGVKAAVRTKIVNIAQLTSNALSLIN 194
           FE+VMSD++TWVSAALT++ TC DGF    V   VK  VR  +V +A+LTSNAL+LIN
Sbjct: 247 FEMVMSDVETWVSAALTNDDTCMDGF--SLVKTAVKDLVRRHVVEVARLTSNALALIN 302

[Close]

BLAST of Tan0006694 vs. TAIR 10
Match: AT1G62770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 136.3 bits (342), Expect = 2.6e-32
Identity = 85/198 (42.93%), Postives = 126/198 (63.64%), Query Frame = 0


Query: 8   QSLILFVVIFQLTVLNPMFTHSAITPH--SSTEFIKSSCSSTAYPRLCFSSLSVHSSSIQ 67
           ++ I  V++  +T+L      S+ TP+   S +FI SSC +T YP LC  +LS +++ I+
Sbjct: 4   KNTIFLVILLSITILQ----SSSATPNRSESDQFIVSSCQTTQYPSLCVHTLSAYATKIR 63

Query: 68  -TSPRLLATAALSVSLSSVKSATAQILKLS-HTHGLPPRDVSALNDCLEELGDSVDSLAS 127
             + + LA  AL +SL+  KS T  + KL+  T     R+  A+ DC+E LG+SVD LA 
Sbjct: 64  HNNDQDLAQTALIISLARAKSVTIFVAKLTKETPKFKRREYLAIKDCIEVLGNSVDRLAQ 123

Query: 128 SISEMPK----LRGHDFELVMSDIQTWVSAALTDETTCSDGFQGKAVNGGVKAAVRTKIV 187
           S+ E+ +    +   DF   MS++QTWVSAALTDETTC DGF  +A+ G VK  +R K+V
Sbjct: 124 SVKELARAGHAVASEDFMWKMSNVQTWVSAALTDETTCLDGFSERAMGGKVKRLIRYKVV 183

Query: 188 NIAQLTSNALSLINRIGE 198
           ++AQ+TSNAL+L+N+  E
Sbjct: 184 HVAQVTSNALALVNQFAE 197

The following BLAST results are available for this feature:

BLAST of Tan0006694 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SB37	3.8e-36	45.45	Pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PMEI7 PE=2 SV=1	[more]
P17407	1.6e-34	44.83	21 kDa protein OS=Daucus carota OX=4039 PE=2 SV=1	[more]
Q9SI74	6.7e-33	46.07	Pectinesterase inhibitor 10 OS=Arabidopsis thaliana OX=3702 GN=PMEI10 PE=2 SV=1	[more]
Q9SI72	3.7e-31	42.93	Pectinesterase inhibitor 9 OS=Arabidopsis thaliana OX=3702 GN=PMEI9 PE=2 SV=1	[more]
Q9STY5	1.8e-30	41.80	Pectinesterase inhibitor 11 OS=Arabidopsis thaliana OX=3702 GN=PMEI11 PE=2 SV=1	[more]

BLAST of Tan0006694 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023551428.1	2.2e-81	86.00	21 kDa protein-like [Cucurbita pepo subsp. pepo]	[more]
XP_022943093.1	2.8e-81	86.00	21 kDa protein-like isoform X3 [Cucurbita moschata]	[more]
KAG6600168.1	3.7e-81	86.00	hypothetical protein SDJN03_05401, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022989139.1	3.7e-81	83.74	21 kDa protein-like [Cucurbita maxima]	[more]
XP_022943092.1	4.9e-81	85.15	21 kDa protein-like isoform X2 [Cucurbita moschata]	[more]

BLAST of Tan0006694 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FWC1	1.4e-81	86.00	21 kDa protein-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111447928 PE=...	[more]
A0A6J1JNI8	1.8e-81	83.74	21 kDa protein-like OS=Cucurbita maxima OX=3661 GN=LOC111486299 PE=4 SV=1	[more]
A0A6J1FS20	2.3e-81	85.15	21 kDa protein-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111447928 PE=...	[more]
A0A6J1FXD1	3.1e-81	84.73	pectinesterase inhibitor 7-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1E4J6	4.9e-79	82.41	21 kDa protein-like OS=Cucurbita moschata OX=3662 GN=LOC111430716 PE=4 SV=1	[more]

BLAST of Tan0006694 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G62360.1	2.4e-46	52.11	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT4G25260.1	2.7e-37	45.45	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT5G62350.1	5.6e-35	48.19	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62760.1	4.7e-34	46.07	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G62770.1	2.6e-32	42.93	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 35..191 e-value: 4.3E-51 score: 185.7
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 12..194 e-value: 7.4E-38 score: 127.6
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 39..191 e-value: 4.4E-37 score: 127.8
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 35..195 e-value: 1.9E-40 score: 140.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 38..195
None	No IPR available	PANTHER	PTHR31080	PECTINESTERASE INHIBITOR-LIKE	coord: 10..195
None	No IPR available	PANTHER	PTHR31080:SF161	21 KDA PROTEIN-LIKE	coord: 10..195
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 42..195 e-value: 1.54091E-41 score: 136.029

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0006694.1	Tan0006694.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0009827	plant-type cell wall modification
biological_process	GO:0051603	proteolysis involved in cellular protein catabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0004222	metalloendopeptidase activity