Tan0006473 (gene) Snake gourd v1

Overview


Name	Tan0006473
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	E3 ubiquitin-protein ligase MBR2-like isoform X2
Location	LG05: 73232611 .. 73235071 (-)
RNA-Seq Expression	Tan0006473
Synteny	Tan0006473

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCTGTTCTTACACATACTTCTACAGTTGGTGAACAAATCAAATTGAGAAGACCCAAAAACCATTTGAGCCAACCCATTTCAGAATCAGATCCAAACCCATCAATCCCTTCACCCCAAATCCCTTCCATAATTCAATCCACTCGCTGCAAATCCACCATTTCTTCTCTTCTGCTCTCGACCTTTTCCAATAACACAACTAGTGGCGCCAATGAATCTTTACCCACGAATAGGAAGAAGAACAACTTCTCTTCTGCAACTTTCAGGGGACTCGGCTGCACTGCCGCCGCTTCTCAGCAGGTTTCGGTTCCGGCTGTAATCCGAACTTCAGCGGATTGGCAGAAGAAGAAAACGAGGAAAAAAAAGCAGAAGAACTCCAATAACAAAACCCAACAAGGAATTGTTGACGGGTCTCATTTTCAACCTAATTCGAATTTCAATTCTGCTAGCTGTTTGGACGCTCAAGATGTTTGGTGTGGCCCTGGAATTGGATTCTCTTCCGATGCGGCTGCTTCTGTGGATTGCGTTGTTGCTAGAAGACATCCTTCCGGAAGAGGAAAAATCGACTTGGAGAAGATGAGTCAGAGGGAGGTACTTGCTTTTCCATTTCTTTTGAATTTTTTTTTGAACTTCTTTCTTCTCTGTCGATGTGTCGCACTGAAAAATCCCTGGTTGGTTATTGTTTAGCATTCGTGTCGATTTCGTGGGTAAGGAAGCATTTGGGTGTTTATTTTATTTCGGCTTTTGTCATTTTATTATTATCAGTATTTCAGTTCAGATATGGGGTTTTTTTTAATCGAACTTTCTCGACTCTGTTTCTTTTCTGCAATAGGGTTCTTCTAAATCAGTCTGAACCTGTTCGACTTCCATTCTTGGAACTTGTTTGTTTTCGTTCTTCCTCGTAAATTTGATTATTTAATGCAAATGGGTATGCGTTAGATTTTGAAGTTCTTCAAATGAAATCTCCCTTTGGCCTTGGCGATATAAAAATGAGTTATCTTTGGTTCATTGTTGATTATTGATAACAGTACATTTTTTAATGCAATTTAACTTCCTCTGGTTTTACCATGACAGCGTTCTTGTTTAGGAAGACGAACAGTGAACCCTGAGACTCTGTTATTTTTGGATTCTGATTCTGATCTTCCAACGGCTCGATCATTGGAAGTGTCTGGAAGTCGGTATTATCGCCATGTTCGACATCCATCCCCTGATGGGCTTGCTGAGGTGAGCCATCATTCATATATGTATTCAGTTTCGTAAGTATCTTACTCGAACAGGTCTATTTTATAGGTTGTATAACTGTGTCAGAGTTCTAAAAATATTAGCTTTACCTGGGTTCTGGCTTTTTTCAGTTCTTTATGTGCTTGAAATCCGCATATGCTATTTGAGGGTGTTGGAAGATTCTGATATTTGGTGAATTAGGTATTATGCTGTTATGAGATGTTGGCAGATGGTAAAAATAAAATGCAGGTTCAGAGAAACAAATCGAACCTAATTTCAAGTTTAATCTGTTTTGCTGAATTATGTTTTTGATTTGGAGTGTTTATTTTTTATCTTATCAGTTGATAGGGGGTTTATTTCCCCATTGGAAGTTGTTGGTTAACGATTCGGATCCTGAAATAAAACATAGATTAGTAGCAGCAATATACATCATACTTCGATTCGTTTTTTCAACGACAATGTTCTGTGTAAATATTGCCATTATCTCCATCCCTTGGCTGTTTAATTTTATTGATAAAGGTGTTCACTAATTCTTCTCCTTCCGGGCGGGAAATATTTTCAGGTTGTGATTTTCTTTTTATGTTCTATGCGAAGTAGTGCTGTTTCACAATGATCATGAGACTGACTTGCTCGCTTGATATGCTATTTAGCAGATTATGATGTTTCAGAGCAGTTTGCTGATGGGTGGAAGGTTCGATATACACGATCAATTTAGGGAACTGCGACTTGACGTCGATAACATGTCATATGAGGTTCATAACTTTCTCCAAATCCTTCTCCCTCTTCTTTCCTGCCACAATGTGATACTCTACTCACAAATCTTTCATATTTCGGGATGGAAACAGGAGCTGCTTGAACTCGGTGAAAGGATTGGCCATGTGAGTACCGGGCTGAAAGAAGATGAGATAGGAAGATGTATTAGAAAAATGAAGCCCCTTGTTGTAAATGAGCTAACATCTCATTTATTATCTCAAATGGATCGGAAGTGCAGCATCTGTCAGGTGAAGTAGAAGAGTTTCAGTTTGCTGGTTTTGATTATCACTTGTATTTGTATCAGTTCTTTCCCACCACATTCTTGACACCTAAAATCATTTTGTACAAGCAGGAGGACTATGAACCAGACGATGAAATGGGTAAGCTGGAATGTGGGCACAGCTATCACATACACTGTATAAAACAGTGGCTTGCACAGAAGAATATGTGCCCAGTCTGTAAGACAGCAGCGGGGGGCCGAGGCTGA

mRNA sequence

ATGCCTGTTCTTACACATACTTCTACAGTTGGTGAACAAATCAAATTGAGAAGACCCAAAAACCATTTGAGCCAACCCATTTCAGAATCAGATCCAAACCCATCAATCCCTTCACCCCAAATCCCTTCCATAATTCAATCCACTCGCTGCAAATCCACCATTTCTTCTCTTCTGCTCTCGACCTTTTCCAATAACACAACTAGTGGCGCCAATGAATCTTTACCCACGAATAGGAAGAAGAACAACTTCTCTTCTGCAACTTTCAGGGGACTCGGCTGCACTGCCGCCGCTTCTCAGCAGGTTTCGGTTCCGGCTGTAATCCGAACTTCAGCGGATTGGCAGAAGAAGAAAACGAGGAAAAAAAAGCAGAAGAACTCCAATAACAAAACCCAACAAGGAATTGTTGACGGGTCTCATTTTCAACCTAATTCGAATTTCAATTCTGCTAGCTGTTTGGACGCTCAAGATGTTTGGTGTGGCCCTGGAATTGGATTCTCTTCCGATGCGGCTGCTTCTGTGGATTGCGTTGTTGCTAGAAGACATCCTTCCGGAAGAGGAAAAATCGACTTGGAGAAGATGAGTCAGAGGGAGCGTTCTTGTTTAGGAAGACGAACAGTGAACCCTGAGACTCTGTTATTTTTGGATTCTGATTCTGATCTTCCAACGGCTCGATCATTGGAAGTGTCTGGAAGTCGGTATTATCGCCATGTTCGACATCCATCCCCTGATGGGCTTGCTGAGATTATGATGTTTCAGAGCAGTTTGCTGATGGGTGGAAGGTTCGATATACACGATCAATTTAGGGAACTGCGACTTGACGTCGATAACATGTCATATGAGGAGCTGCTTGAACTCGGTGAAAGGATTGGCCATGTGAGTACCGGGCTGAAAGAAGATGAGATAGGAAGATGTATTAGAAAAATGAAGCCCCTTGTTGTAAATGAGCTAACATCTCATTTATTATCTCAAATGGATCGGAAGTGCAGCATCTGTCAGGAGGACTATGAACCAGACGATGAAATGGGTAAGCTGGAATGTGGGCACAGCTATCACATACACTGTATAAAACAGTGGCTTGCACAGAAGAATATGTGCCCAGTCTGTAAGACAGCAGCGGGGGGCCGAGGCTGA

Coding sequence (CDS)

ATGCCTGTTCTTACACATACTTCTACAGTTGGTGAACAAATCAAATTGAGAAGACCCAAAAACCATTTGAGCCAACCCATTTCAGAATCAGATCCAAACCCATCAATCCCTTCACCCCAAATCCCTTCCATAATTCAATCCACTCGCTGCAAATCCACCATTTCTTCTCTTCTGCTCTCGACCTTTTCCAATAACACAACTAGTGGCGCCAATGAATCTTTACCCACGAATAGGAAGAAGAACAACTTCTCTTCTGCAACTTTCAGGGGACTCGGCTGCACTGCCGCCGCTTCTCAGCAGGTTTCGGTTCCGGCTGTAATCCGAACTTCAGCGGATTGGCAGAAGAAGAAAACGAGGAAAAAAAAGCAGAAGAACTCCAATAACAAAACCCAACAAGGAATTGTTGACGGGTCTCATTTTCAACCTAATTCGAATTTCAATTCTGCTAGCTGTTTGGACGCTCAAGATGTTTGGTGTGGCCCTGGAATTGGATTCTCTTCCGATGCGGCTGCTTCTGTGGATTGCGTTGTTGCTAGAAGACATCCTTCCGGAAGAGGAAAAATCGACTTGGAGAAGATGAGTCAGAGGGAGCGTTCTTGTTTAGGAAGACGAACAGTGAACCCTGAGACTCTGTTATTTTTGGATTCTGATTCTGATCTTCCAACGGCTCGATCATTGGAAGTGTCTGGAAGTCGGTATTATCGCCATGTTCGACATCCATCCCCTGATGGGCTTGCTGAGATTATGATGTTTCAGAGCAGTTTGCTGATGGGTGGAAGGTTCGATATACACGATCAATTTAGGGAACTGCGACTTGACGTCGATAACATGTCATATGAGGAGCTGCTTGAACTCGGTGAAAGGATTGGCCATGTGAGTACCGGGCTGAAAGAAGATGAGATAGGAAGATGTATTAGAAAAATGAAGCCCCTTGTTGTAAATGAGCTAACATCTCATTTATTATCTCAAATGGATCGGAAGTGCAGCATCTGTCAGGAGGACTATGAACCAGACGATGAAATGGGTAAGCTGGAATGTGGGCACAGCTATCACATACACTGTATAAAACAGTGGCTTGCACAGAAGAATATGTGCCCAGTCTGTAAGACAGCAGCGGGGGGCCGAGGCTGA

Protein sequence

MPVLTHTSTVGEQIKLRRPKNHLSQPISESDPNPSIPSPQIPSIIQSTRCKSTISSLLLSTFSNNTTSGANESLPTNRKKNNFSSATFRGLGCTAAASQQVSVPAVIRTSADWQKKKTRKKKQKNSNNKTQQGIVDGSHFQPNSNFNSASCLDAQDVWCGPGIGFSSDAAASVDCVVARRHPSGRGKIDLEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSGSRYYRHVRHPSPDGLAEIMMFQSSLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGRCIRKMKPLVVNELTSHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIKQWLAQKNMCPVCKTAAGGRG

Homology

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BLAST of Tan0006473 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 7.7e-30
Identity = 69/149 (46.31%), Postives = 90/149 (60.40%), Query Frame = 0


Query: 224 RSLEVSGSRYYRHVRHPSPDGLAEIMMFQSSLLMGGRFDIHDQFRELRLDVDNMSYEELL 283
           R+  +S  R   H      +   E  M    L+  G  D+HD+ RE+RLDVDNMSYEELL
Sbjct: 553 RNQIISEIRQVLHAMRRGENLRVEDYMVFDPLIYQGMTDMHDRHREMRLDVDNMSYEELL 612

Query: 284 ELGERIGHVSTGLKEDEIGRCIRKMKPLVVNELTSHLLSQMDRKCSICQEDYEPDDEMGK 343
            LGERIG VSTGL E+ I + +++ K    +  +S  L Q    C ICQE+Y   D +G 
Sbjct: 613 ALGERIGDVSTGLSEEVILKAMKQHKH-TSSSPSSVELHQNIEPCCICQEEYVEGDNLGT 672

Query: 344 LECGHSYHIHCIKQWLAQKNMCPVCKTAA 373
           L+CGH +H  CIKQW+  KN+CP+CKT A
Sbjct: 673 LKCGHEFHKDCIKQWVMIKNLCPICKTEA 700

[Close]

BLAST of Tan0006473 vs. ExPASy Swiss-Prot
Match: O49500 (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 2.5e-28
Identity = 81/214 (37.85%), Postives = 117/214 (54.67%), Query Frame = 0


Query: 160 GPGIGFSSDAAASVDCVVARRHPSGRGKIDLEKMSQRERSCLGRRTVNPETLLFLDSDSD 219
           GP +  SS+ AA+     +R H S + +  L  + +R+   L  R +       L +D+D
Sbjct: 466 GPSV--SSNEAAAPSGSSSRSHRSRQRRSGL--LLERQNDHLHLRHLGRS----LAADND 525

Query: 220 LPTARSLEVSGSRYYRHVRHPSPDGLAEIMMFQSSLLMGGRFDIHDQFRELRLDVDNMSY 279
               R+  +S  R          +   E  M    L+  G  ++HD+ R++RLDVDNMSY
Sbjct: 526 ---GRNRLISEIRQVLSAMRRGENLRFEDYMVFDPLIYQGMAEMHDRHRDMRLDVDNMSY 585

Query: 280 EELLELGERIGHVSTGLKEDEIGRCIRKMKPLVVNELTSHLLSQMDRK-CSICQEDYEPD 339
           EELL LGERIG VSTGL E+ I + +++ K       +S   S  D + C +CQE+Y   
Sbjct: 586 EELLALGERIGDVSTGLSEEVILKVMKQHK-----HTSSAAGSHQDMEPCCVCQEEYAEG 645

Query: 340 DEMGKLECGHSYHIHCIKQWLAQKNMCPVCKTAA 373
           D++G L CGH +H  C+KQWL  KN+CP+CKT A
Sbjct: 646 DDLGTLGCGHEFHTACVKQWLMLKNLCPICKTVA 663

[Close]

BLAST of Tan0006473 vs. ExPASy Swiss-Prot
Match: Q9FMM4 (Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RHG1A PE=2 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 2.5e-28
Identity = 61/127 (48.03%), Postives = 84/127 (66.14%), Query Frame = 0


Query: 245 LAEIMMFQSSLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGRC 304
           L ++M+   S+L  G    HD++R++RLDVDNMSYEELL L ERIG V TG+ E+ I   
Sbjct: 560 LEDVMLLNHSVLFDGATG-HDRYRDMRLDVDNMSYEELLALEERIGDVCTGVNEETISNR 619

Query: 305 IRKMKPLVVNELTSHLLSQMDRK-CSICQEDYEPDDEMGKLECGHSYHIHCIKQWLAQKN 364
           +++ K        S+  S  D + C +CQE+Y   ++MG LECGH +H  CIK+WL QKN
Sbjct: 620 LKQRK------YKSNTKSPQDAEPCCVCQEEYTEGEDMGTLECGHEFHSQCIKEWLKQKN 679

Query: 365 MCPVCKT 371
           +CP+CKT
Sbjct: 680 LCPICKT 679

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BLAST of Tan0006473 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 127.5 bits (319), Expect = 3.2e-28
Identity = 60/121 (49.59%), Postives = 83/121 (68.60%), Query Frame = 0


Query: 254 SLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGRCIRKMKPLVV 313
           S+  GG  DIHD+ R++RLD+DNMSYEELL L ERIG+VSTGL E+E+ + +++ K    
Sbjct: 551 SIFYGG-VDIHDRHRDMRLDIDNMSYEELLALEERIGNVSTGLSEEEVTKLLKQRK---- 610

Query: 314 NELTSHLL--SQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIKQWLAQKNMCPVCKTA 373
              +S  L  S  +  C ICQE+Y   D++G L+CGH +H+ C++QWL  KN CP+CK  
Sbjct: 611 --FSSWRLEASVEEEPCCICQEEYVDGDDLGTLDCGHDFHVGCVRQWLVVKNTCPICKNT 664

[Close]

BLAST of Tan0006473 vs. ExPASy Swiss-Prot
Match: Q5QLR5 (Probable E3 ubiquitin-protein ligase ZFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=ZFP1 PE=1 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 1.3e-21
Identity = 51/118 (43.22%), Postives = 76/118 (64.41%), Query Frame = 0


Query: 262 DIHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGRCIRKMKPLVVNELTSHLL 321
           ++ D+ R++RLD+D+M+YEELL L E+IG V+TGL +  I   ++    +  +   S+  
Sbjct: 402 NVVDEHRDMRLDIDSMTYEELLALEEQIGDVNTGLAKSYIVEKLKTSLFVPGSSCMSNKS 461

Query: 322 SQMDRK---CSICQEDYEPDDEMGKLECGHSYHIHCIKQWLAQKNMCPVCKTAAGGRG 377
           S+   +   C ICQE+Y+  + +G L+CGH YH  CIKQWL  KN+CP+CKT A   G
Sbjct: 462 SESSMENDACIICQEEYQVKECIGTLDCGHRYHEDCIKQWLMVKNLCPICKTTALSTG 519

[Close]

BLAST of Tan0006473 vs. NCBI nr
Match: XP_038894454.1 (E3 ubiquitin-protein ligase MBR2 isoform X2 [Benincasa hispida])

HSP 1 Score: 679.1 bits (1751), Expect = 2.2e-191
Identity = 345/383 (90.08%), Postives = 358/383 (93.47%), Query Frame = 0


Query: 1   MPVLTHTSTVGEQIKLRRPK---NHLSQPISESDPNPSIPSPQIPSIIQSTRCKSTISSL 60
           MPV+THTSTVGE +KLRRPK   NHL+QPISESDPNPS     IPSIIQSTRCKSTISSL
Sbjct: 1   MPVVTHTSTVGEHMKLRRPKSILNHLNQPISESDPNPS----SIPSIIQSTRCKSTISSL 60

Query: 61  LLSTFSNNTTSGANESLP----TNRKKNNFSSATFRGLGCTAAASQQVSVPAVIRTSADW 120
           LLSTFSNNTTSG NE+LP    TNRKKNNFSSAT RGLGCT AASQQVSVPAVIRTSADW
Sbjct: 61  LLSTFSNNTTSGGNEALPSSIITNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADW 120

Query: 121 QKKKTRKKKQKNSNNKTQQGIVDGSHFQPNSNFNSASCLDAQDVWCGPGIGFSSDAAASV 180
           ++KKTRKKKQKNS NKTQQGIVD SHFQPNSN NSASCLDAQDVWCGPGIGFS+DAAASV
Sbjct: 121 ERKKTRKKKQKNSKNKTQQGIVDASHFQPNSNINSASCLDAQDVWCGPGIGFSADAAASV 180

Query: 181 DCVVARRHPSGRGKIDLEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSGSRY 240
           DCVVARRH SGRGKIDLEK++QRERSCLGRRTVNPETLLFLDSDSDLPTARSLE+S SRY
Sbjct: 181 DCVVARRHASGRGKIDLEKITQRERSCLGRRTVNPETLLFLDSDSDLPTARSLELSRSRY 240

Query: 241 YRHVRHPSPDGLAEIMMFQSSLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHVS 300
           YRHVRHPSPDGLAEIMMFQSSLLMGGRFD+HDQFRELRLDVDNMSYEELLELGERIGHVS
Sbjct: 241 YRHVRHPSPDGLAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHVS 300

Query: 301 TGLKEDEIGRCIRKMKPLVVNELTSHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIH 360
           TGLKEDEI RCIRKMKPLVVNEL +HLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIH
Sbjct: 301 TGLKEDEIVRCIRKMKPLVVNELATHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIH 360

Query: 361 CIKQWLAQKNMCPVCKTAAGGRG 377
           CIKQWL+QKN CPVCKTAA GRG
Sbjct: 361 CIKQWLSQKNTCPVCKTAAVGRG 379

[Close]

BLAST of Tan0006473 vs. NCBI nr
Match: XP_038894453.1 (E3 ubiquitin-protein ligase MBR2 isoform X1 [Benincasa hispida])

HSP 1 Score: 674.5 bits (1739), Expect = 5.4e-190
Identity = 345/384 (89.84%), Postives = 358/384 (93.23%), Query Frame = 0


Query: 1   MPVLTHTSTVGEQIKLRRPK---NHLSQPISESDPNPSIPSPQIPSIIQSTRCKSTISSL 60
           MPV+THTSTVGE +KLRRPK   NHL+QPISESDPNPS     IPSIIQSTRCKSTISSL
Sbjct: 1   MPVVTHTSTVGEHMKLRRPKSILNHLNQPISESDPNPS----SIPSIIQSTRCKSTISSL 60

Query: 61  LLSTFSNNTTSGANESLP----TNRKKNNFSSATFRGLGCTAAASQQVSVPAVIRTSADW 120
           LLSTFSNNTTSG NE+LP    TNRKKNNFSSAT RGLGCT AASQQVSVPAVIRTSADW
Sbjct: 61  LLSTFSNNTTSGGNEALPSSIITNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSADW 120

Query: 121 QKKKTRKKKQKNSNNKTQQGIVDGSHFQPNSNFNSASCLDAQDVWCGPGIGFSSDAAASV 180
           ++KKTRKKKQKNS NKTQQGIVD SHFQPNSN NSASCLDAQDVWCGPGIGFS+DAAASV
Sbjct: 121 ERKKTRKKKQKNSKNKTQQGIVDASHFQPNSNINSASCLDAQDVWCGPGIGFSADAAASV 180

Query: 181 DCVVARRHPSGRGKIDLEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSGSRY 240
           DCVVARRH SGRGKIDLEK++QRERSCLGRRTVNPETLLFLDSDSDLPTARSLE+S SRY
Sbjct: 181 DCVVARRHASGRGKIDLEKITQRERSCLGRRTVNPETLLFLDSDSDLPTARSLELSRSRY 240

Query: 241 YRHVRHPSPDGLAE-IMMFQSSLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHV 300
           YRHVRHPSPDGLAE IMMFQSSLLMGGRFD+HDQFRELRLDVDNMSYEELLELGERIGHV
Sbjct: 241 YRHVRHPSPDGLAEQIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHV 300

Query: 301 STGLKEDEIGRCIRKMKPLVVNELTSHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHI 360
           STGLKEDEI RCIRKMKPLVVNEL +HLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHI
Sbjct: 301 STGLKEDEIVRCIRKMKPLVVNELATHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHI 360

Query: 361 HCIKQWLAQKNMCPVCKTAAGGRG 377
           HCIKQWL+QKN CPVCKTAA GRG
Sbjct: 361 HCIKQWLSQKNTCPVCKTAAVGRG 380

[Close]

BLAST of Tan0006473 vs. NCBI nr
Match: XP_022137196.1 (uncharacterized protein LOC111008721 isoform X2 [Momordica charantia])

HSP 1 Score: 665.6 bits (1716), Expect = 2.5e-187
Identity = 340/380 (89.47%), Postives = 352/380 (92.63%), Query Frame = 0


Query: 1   MPVLTHTSTVGEQIKLRRPKNHLSQPISESDPNPSIPSPQIPSIIQSTRCKSTISSLLLS 60
           MPV+T T+TVGE IKLRRP+ HLSQPISESDPNPS     IPSIIQSTRCKSTISSLLLS
Sbjct: 1   MPVVTQTTTVGEHIKLRRPRGHLSQPISESDPNPS-----IPSIIQSTRCKSTISSLLLS 60

Query: 61  TFSNNTTSGANESLP----TNRKKNNFSSATFRGLGCTAAASQQVSVPAVIRTSADWQKK 120
           TFSN   SGA ESLP    TNRKK+NFSSATFRGLGCTA+ASQQVSVPAVIRTSADWQKK
Sbjct: 61  TFSN---SGATESLPSSSVTNRKKSNFSSATFRGLGCTASASQQVSVPAVIRTSADWQKK 120

Query: 121 KTRKKKQKNSNNKTQQGIVDGSHFQPNSNFNSASCLDAQDVWCGPGIGFSSDAAASVDCV 180
           KTRKKKQK+S NKTQQGI DG++F PNSNFNSASCLDAQDVWCGPGIGFS DAAASVDCV
Sbjct: 121 KTRKKKQKSSKNKTQQGIGDGTYFHPNSNFNSASCLDAQDVWCGPGIGFSPDAAASVDCV 180

Query: 181 VARRHPSGRGKIDLEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSGSRYYRH 240
           VARRH SGRGKID+EKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVS SRYYRH
Sbjct: 181 VARRHASGRGKIDVEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSRSRYYRH 240

Query: 241 VRHPSPDGLAEIMMFQSSLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHVSTGL 300
           VRHPSPDGLAEIMMFQSSLLMGGRFD HDQFRELRLDVDNMSYEELLELGERIGHVSTGL
Sbjct: 241 VRHPSPDGLAEIMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTGL 300

Query: 301 KEDEIGRCIRKMKPLVVNELTSHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIK 360
           KEDEIG+CIRKMKP +VNEL SHLLSQ+DRKCSICQEDYE DDEMGKLECGHSYHIHCIK
Sbjct: 301 KEDEIGKCIRKMKPFIVNELASHLLSQVDRKCSICQEDYEVDDEMGKLECGHSYHIHCIK 360

Query: 361 QWLAQKNMCPVCKTAAGGRG 377
           QWLAQKNMCPVCKTA  GRG
Sbjct: 361 QWLAQKNMCPVCKTAVVGRG 372

[Close]

BLAST of Tan0006473 vs. NCBI nr
Match: XP_022137195.1 (uncharacterized protein LOC111008721 isoform X1 [Momordica charantia])

HSP 1 Score: 661.0 bits (1704), Expect = 6.2e-186
Identity = 340/381 (89.24%), Postives = 352/381 (92.39%), Query Frame = 0


Query: 1   MPVLTHTSTVGEQIKLRRPKNHLSQPISESDPNPSIPSPQIPSIIQSTRCKSTISSLLLS 60
           MPV+T T+TVGE IKLRRP+ HLSQPISESDPNPS     IPSIIQSTRCKSTISSLLLS
Sbjct: 1   MPVVTQTTTVGEHIKLRRPRGHLSQPISESDPNPS-----IPSIIQSTRCKSTISSLLLS 60

Query: 61  TFSNNTTSGANESLP----TNRKKNNFSSATFRGLGCTAAASQQVSVPAVIRTSADWQKK 120
           TFSN   SGA ESLP    TNRKK+NFSSATFRGLGCTA+ASQQVSVPAVIRTSADWQKK
Sbjct: 61  TFSN---SGATESLPSSSVTNRKKSNFSSATFRGLGCTASASQQVSVPAVIRTSADWQKK 120

Query: 121 KTRKKKQKNSNNKTQQGIVDGSHFQPNSNFNSASCLDAQDVWCGPGIGFSSDAAASVDCV 180
           KTRKKKQK+S NKTQQGI DG++F PNSNFNSASCLDAQDVWCGPGIGFS DAAASVDCV
Sbjct: 121 KTRKKKQKSSKNKTQQGIGDGTYFHPNSNFNSASCLDAQDVWCGPGIGFSPDAAASVDCV 180

Query: 181 VARRHPSGRGKIDLEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSGSRYYRH 240
           VARRH SGRGKID+EKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVS SRYYRH
Sbjct: 181 VARRHASGRGKIDVEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSRSRYYRH 240

Query: 241 VRHPSPDGLAE-IMMFQSSLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHVSTG 300
           VRHPSPDGLAE IMMFQSSLLMGGRFD HDQFRELRLDVDNMSYEELLELGERIGHVSTG
Sbjct: 241 VRHPSPDGLAEQIMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTG 300

Query: 301 LKEDEIGRCIRKMKPLVVNELTSHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCI 360
           LKEDEIG+CIRKMKP +VNEL SHLLSQ+DRKCSICQEDYE DDEMGKLECGHSYHIHCI
Sbjct: 301 LKEDEIGKCIRKMKPFIVNELASHLLSQVDRKCSICQEDYEVDDEMGKLECGHSYHIHCI 360

Query: 361 KQWLAQKNMCPVCKTAAGGRG 377
           KQWLAQKNMCPVCKTA  GRG
Sbjct: 361 KQWLAQKNMCPVCKTAVVGRG 373

[Close]

BLAST of Tan0006473 vs. NCBI nr
Match: XP_004137488.1 (E3 ubiquitin-protein ligase MBR2 isoform X2 [Cucumis sativus])

HSP 1 Score: 657.5 bits (1695), Expect = 6.8e-185
Identity = 337/384 (87.76%), Postives = 355/384 (92.45%), Query Frame = 0


Query: 1   MPVLTHTSTVGEQIKLRRPK---NHLSQPISESDPNPSIPSPQIPSIIQSTRCKSTISSL 60
           MPV+    TVGE +KLRRP+   +HL+QPISESDPNPS     IPSIIQSTRCKSTISSL
Sbjct: 1   MPVV----TVGEHMKLRRPRSDLSHLNQPISESDPNPS-----IPSIIQSTRCKSTISSL 60

Query: 61  LLSTFSNNTTSGANESLP-----TNRKKNNFSSATFRGLGCTAAASQQVSVPAVIRTSAD 120
           LLSTFSNNTTSG NESLP     TNRKKNNFSSAT RGLGCT AASQQVSVPAVIRTSAD
Sbjct: 61  LLSTFSNNTTSGPNESLPSSIITTNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSAD 120

Query: 121 WQKKKTRKKKQKNSNNKTQQGIVDGSHFQPNSNFNSASCLDAQDVWCGPGIGFSSDAAAS 180
           W+KKKTRKKKQK+S NKTQQGIVD SHFQPNS+ NSASCLDAQDVWCGPGIGFS+DAAAS
Sbjct: 121 WEKKKTRKKKQKSSKNKTQQGIVDASHFQPNSSMNSASCLDAQDVWCGPGIGFSADAAAS 180

Query: 181 VDCVVARRHPSGRGKIDLEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSGSR 240
           VDCVVARRH SGRGKIDLEK++QRERSCLGRRTV+PETLLFLDSDS++PTARSLE+S SR
Sbjct: 181 VDCVVARRHASGRGKIDLEKINQRERSCLGRRTVSPETLLFLDSDSEIPTARSLELSRSR 240

Query: 241 YYRHVRHPSPDGLAEIMMFQSSLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHV 300
           YYRHVRHPSPDGLAEIMMFQSSLLMGGRFD+HDQFRELRLDVDNMSYEELLELGERIGHV
Sbjct: 241 YYRHVRHPSPDGLAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHV 300

Query: 301 STGLKEDEIGRCIRKMKPLVVNELTSHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHI 360
           STGLK+DEIGRCIRKMKP VVNELT+HLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHI
Sbjct: 301 STGLKDDEIGRCIRKMKPHVVNELTTHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHI 360

Query: 361 HCIKQWLAQKNMCPVCKTAAGGRG 377
           HCIKQWLAQKN CPVCKTAA GRG
Sbjct: 361 HCIKQWLAQKNTCPVCKTAAVGRG 375

[Close]

BLAST of Tan0006473 vs. ExPASy TrEMBL
Match: A0A6J1C5U0 (uncharacterized protein LOC111008721 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111008721 PE=4 SV=1)

HSP 1 Score: 665.6 bits (1716), Expect = 1.2e-187
Identity = 340/380 (89.47%), Postives = 352/380 (92.63%), Query Frame = 0


Query: 1   MPVLTHTSTVGEQIKLRRPKNHLSQPISESDPNPSIPSPQIPSIIQSTRCKSTISSLLLS 60
           MPV+T T+TVGE IKLRRP+ HLSQPISESDPNPS     IPSIIQSTRCKSTISSLLLS
Sbjct: 1   MPVVTQTTTVGEHIKLRRPRGHLSQPISESDPNPS-----IPSIIQSTRCKSTISSLLLS 60

Query: 61  TFSNNTTSGANESLP----TNRKKNNFSSATFRGLGCTAAASQQVSVPAVIRTSADWQKK 120
           TFSN   SGA ESLP    TNRKK+NFSSATFRGLGCTA+ASQQVSVPAVIRTSADWQKK
Sbjct: 61  TFSN---SGATESLPSSSVTNRKKSNFSSATFRGLGCTASASQQVSVPAVIRTSADWQKK 120

Query: 121 KTRKKKQKNSNNKTQQGIVDGSHFQPNSNFNSASCLDAQDVWCGPGIGFSSDAAASVDCV 180
           KTRKKKQK+S NKTQQGI DG++F PNSNFNSASCLDAQDVWCGPGIGFS DAAASVDCV
Sbjct: 121 KTRKKKQKSSKNKTQQGIGDGTYFHPNSNFNSASCLDAQDVWCGPGIGFSPDAAASVDCV 180

Query: 181 VARRHPSGRGKIDLEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSGSRYYRH 240
           VARRH SGRGKID+EKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVS SRYYRH
Sbjct: 181 VARRHASGRGKIDVEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSRSRYYRH 240

Query: 241 VRHPSPDGLAEIMMFQSSLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHVSTGL 300
           VRHPSPDGLAEIMMFQSSLLMGGRFD HDQFRELRLDVDNMSYEELLELGERIGHVSTGL
Sbjct: 241 VRHPSPDGLAEIMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTGL 300

Query: 301 KEDEIGRCIRKMKPLVVNELTSHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIK 360
           KEDEIG+CIRKMKP +VNEL SHLLSQ+DRKCSICQEDYE DDEMGKLECGHSYHIHCIK
Sbjct: 301 KEDEIGKCIRKMKPFIVNELASHLLSQVDRKCSICQEDYEVDDEMGKLECGHSYHIHCIK 360

Query: 361 QWLAQKNMCPVCKTAAGGRG 377
           QWLAQKNMCPVCKTA  GRG
Sbjct: 361 QWLAQKNMCPVCKTAVVGRG 372

[Close]

BLAST of Tan0006473 vs. ExPASy TrEMBL
Match: A0A6J1C605 (uncharacterized protein LOC111008721 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111008721 PE=4 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 3.0e-186
Identity = 340/381 (89.24%), Postives = 352/381 (92.39%), Query Frame = 0


Query: 1   MPVLTHTSTVGEQIKLRRPKNHLSQPISESDPNPSIPSPQIPSIIQSTRCKSTISSLLLS 60
           MPV+T T+TVGE IKLRRP+ HLSQPISESDPNPS     IPSIIQSTRCKSTISSLLLS
Sbjct: 1   MPVVTQTTTVGEHIKLRRPRGHLSQPISESDPNPS-----IPSIIQSTRCKSTISSLLLS 60

Query: 61  TFSNNTTSGANESLP----TNRKKNNFSSATFRGLGCTAAASQQVSVPAVIRTSADWQKK 120
           TFSN   SGA ESLP    TNRKK+NFSSATFRGLGCTA+ASQQVSVPAVIRTSADWQKK
Sbjct: 61  TFSN---SGATESLPSSSVTNRKKSNFSSATFRGLGCTASASQQVSVPAVIRTSADWQKK 120

Query: 121 KTRKKKQKNSNNKTQQGIVDGSHFQPNSNFNSASCLDAQDVWCGPGIGFSSDAAASVDCV 180
           KTRKKKQK+S NKTQQGI DG++F PNSNFNSASCLDAQDVWCGPGIGFS DAAASVDCV
Sbjct: 121 KTRKKKQKSSKNKTQQGIGDGTYFHPNSNFNSASCLDAQDVWCGPGIGFSPDAAASVDCV 180

Query: 181 VARRHPSGRGKIDLEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSGSRYYRH 240
           VARRH SGRGKID+EKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVS SRYYRH
Sbjct: 181 VARRHASGRGKIDVEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSRSRYYRH 240

Query: 241 VRHPSPDGLAE-IMMFQSSLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHVSTG 300
           VRHPSPDGLAE IMMFQSSLLMGGRFD HDQFRELRLDVDNMSYEELLELGERIGHVSTG
Sbjct: 241 VRHPSPDGLAEQIMMFQSSLLMGGRFDTHDQFRELRLDVDNMSYEELLELGERIGHVSTG 300

Query: 301 LKEDEIGRCIRKMKPLVVNELTSHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCI 360
           LKEDEIG+CIRKMKP +VNEL SHLLSQ+DRKCSICQEDYE DDEMGKLECGHSYHIHCI
Sbjct: 301 LKEDEIGKCIRKMKPFIVNELASHLLSQVDRKCSICQEDYEVDDEMGKLECGHSYHIHCI 360

Query: 361 KQWLAQKNMCPVCKTAAGGRG 377
           KQWLAQKNMCPVCKTA  GRG
Sbjct: 361 KQWLAQKNMCPVCKTAVVGRG 373

[Close]

BLAST of Tan0006473 vs. ExPASy TrEMBL
Match: A0A0A0LQF5 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G042600 PE=4 SV=1)

HSP 1 Score: 657.5 bits (1695), Expect = 3.3e-185
Identity = 337/384 (87.76%), Postives = 355/384 (92.45%), Query Frame = 0


Query: 1   MPVLTHTSTVGEQIKLRRPK---NHLSQPISESDPNPSIPSPQIPSIIQSTRCKSTISSL 60
           MPV+    TVGE +KLRRP+   +HL+QPISESDPNPS     IPSIIQSTRCKSTISSL
Sbjct: 1   MPVV----TVGEHMKLRRPRSDLSHLNQPISESDPNPS-----IPSIIQSTRCKSTISSL 60

Query: 61  LLSTFSNNTTSGANESLP-----TNRKKNNFSSATFRGLGCTAAASQQVSVPAVIRTSAD 120
           LLSTFSNNTTSG NESLP     TNRKKNNFSSAT RGLGCT AASQQVSVPAVIRTSAD
Sbjct: 61  LLSTFSNNTTSGPNESLPSSIITTNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSAD 120

Query: 121 WQKKKTRKKKQKNSNNKTQQGIVDGSHFQPNSNFNSASCLDAQDVWCGPGIGFSSDAAAS 180
           W+KKKTRKKKQK+S NKTQQGIVD SHFQPNS+ NSASCLDAQDVWCGPGIGFS+DAAAS
Sbjct: 121 WEKKKTRKKKQKSSKNKTQQGIVDASHFQPNSSMNSASCLDAQDVWCGPGIGFSADAAAS 180

Query: 181 VDCVVARRHPSGRGKIDLEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSGSR 240
           VDCVVARRH SGRGKIDLEK++QRERSCLGRRTV+PETLLFLDSDS++PTARSLE+S SR
Sbjct: 181 VDCVVARRHASGRGKIDLEKINQRERSCLGRRTVSPETLLFLDSDSEIPTARSLELSRSR 240

Query: 241 YYRHVRHPSPDGLAEIMMFQSSLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHV 300
           YYRHVRHPSPDGLAEIMMFQSSLLMGGRFD+HDQFRELRLDVDNMSYEELLELGERIGHV
Sbjct: 241 YYRHVRHPSPDGLAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHV 300

Query: 301 STGLKEDEIGRCIRKMKPLVVNELTSHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHI 360
           STGLK+DEIGRCIRKMKP VVNELT+HLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHI
Sbjct: 301 STGLKDDEIGRCIRKMKPHVVNELTTHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHI 360

Query: 361 HCIKQWLAQKNMCPVCKTAAGGRG 377
           HCIKQWLAQKN CPVCKTAA GRG
Sbjct: 361 HCIKQWLAQKNTCPVCKTAAVGRG 375

[Close]

BLAST of Tan0006473 vs. ExPASy TrEMBL
Match: A0A6J1GUE7 (E3 ubiquitin-protein ligase MBR2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457028 PE=4 SV=1)

HSP 1 Score: 651.0 bits (1678), Expect = 3.1e-183
Identity = 332/380 (87.37%), Postives = 349/380 (91.84%), Query Frame = 0


Query: 1   MPVLTHTSTVGEQIKLRRPKNHLSQPISESDPNPSIPSPQIPSIIQSTRCKSTISSLLLS 60
           MPV+T  ST+ E IKLRRP++HL QPISESDPNPS     IPSI+QSTRCKSTISSLLLS
Sbjct: 1   MPVVTRASTMDEHIKLRRPRSHLRQPISESDPNPS-----IPSIVQSTRCKSTISSLLLS 60

Query: 61  TFSNNTTSGANESL----PTNRKKNNFSSATFRGLGCTAAASQQVSVPAVIRTSADWQKK 120
           TF+NN T+G NESL    PTNRKK +FSSATFRGLGCT+AASQQV+VPAVIRTSADWQKK
Sbjct: 61  TFTNNATNGTNESLPSSVPTNRKKTHFSSATFRGLGCTSAASQQVTVPAVIRTSADWQKK 120

Query: 121 KTRKKKQKNSNNKTQQGIVDGSHFQPNSNFNSASCLDAQDVWCGPGIGFSSDAAASVDCV 180
            TRKKKQKNS NKT QGIVDGSHFQ NS FNSASCLDAQDVWCGPGIGFS+DAAASVDCV
Sbjct: 121 NTRKKKQKNSKNKTHQGIVDGSHFQHNSPFNSASCLDAQDVWCGPGIGFSADAAASVDCV 180

Query: 181 VARRHPSGRGKIDLEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSGSRYYRH 240
           VARRH SGRGKIDLEKM+QRERS LGRRTVNPETLL L+SDSDLPTARSLEVS SRYYRH
Sbjct: 181 VARRHASGRGKIDLEKMTQRERSYLGRRTVNPETLLLLESDSDLPTARSLEVSRSRYYRH 240

Query: 241 VRHPSPDGLAEIMMFQSSLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHVSTGL 300
           VRHPSPDGLAEIMMFQSSLLMGGRFDI DQFRELRLDVD+MSYEELLELGERIGHVSTGL
Sbjct: 241 VRHPSPDGLAEIMMFQSSLLMGGRFDIRDQFRELRLDVDDMSYEELLELGERIGHVSTGL 300

Query: 301 KEDEIGRCIRKMKPLVVNELTSHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIK 360
           KEDEIGRCIRK KPLVVNEL+S+LLSQMDRKCSICQEDYEPDDEMG+L CGHSYHIHCIK
Sbjct: 301 KEDEIGRCIRKTKPLVVNELSSYLLSQMDRKCSICQEDYEPDDEMGRLGCGHSYHIHCIK 360

Query: 361 QWLAQKNMCPVCKTAAGGRG 377
           QWLAQKN CPVCKTAA GRG
Sbjct: 361 QWLAQKNTCPVCKTAAVGRG 375

[Close]

BLAST of Tan0006473 vs. ExPASy TrEMBL
Match: A0A5D3BIX1 (E3 ubiquitin-protein ligase MBR2 isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold248G002180 PE=4 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 6.9e-183
Identity = 334/384 (86.98%), Postives = 352/384 (91.67%), Query Frame = 0


Query: 1   MPVLTHTSTVGEQIKLRRPK---NHLSQPISESDPNPSIPSPQIPSIIQSTRCKSTISSL 60
           MPV+    TVGE +KLRRP+   +HL+QPISESDPNPS     IPSIIQSTRCKSTISSL
Sbjct: 1   MPVV----TVGEHMKLRRPRSDLSHLNQPISESDPNPS-----IPSIIQSTRCKSTISSL 60

Query: 61  LLSTFSNNTTSGANESLP-----TNRKKNNFSSATFRGLGCTAAASQQVSVPAVIRTSAD 120
           LLSTFSNNTTSG NESLP     TNRKKNNFSSAT RGLGCT AASQQVSVPAVIRTSAD
Sbjct: 61  LLSTFSNNTTSGPNESLPSSIITTNRKKNNFSSATLRGLGCTTAASQQVSVPAVIRTSAD 120

Query: 121 WQKKKTRKKKQKNSNNKTQQGIVDGSHFQPNSNFNSASCLDAQDVWCGPGIGFSSDAAAS 180
           W+ KKTRKKKQK+S NKTQQG++D SHFQPNS+ NSASCLDAQDVWCGPGIGFS+DAAAS
Sbjct: 121 WENKKTRKKKQKSSKNKTQQGVLDASHFQPNSSMNSASCLDAQDVWCGPGIGFSADAAAS 180

Query: 181 VDCVVARRHPSGRGKIDLEKMSQRERSCLGRRTVNPETLLFLDSDSDLPTARSLEVSGSR 240
           VDCVVARRH SGRGKIDLEK++QRERSCLGRRTV+PETLLFLDSDSDL TARSLE+S +R
Sbjct: 181 VDCVVARRHASGRGKIDLEKINQRERSCLGRRTVSPETLLFLDSDSDLSTARSLELSRTR 240

Query: 241 YYRHVRHPSPDGLAEIMMFQSSLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHV 300
           YYRHVRHPSPDGLAEIMMFQSSLLMGGRFD+HDQFRELRLDVDNMSYEELLELGERIGHV
Sbjct: 241 YYRHVRHPSPDGLAEIMMFQSSLLMGGRFDLHDQFRELRLDVDNMSYEELLELGERIGHV 300

Query: 301 STGLKEDEIGRCIRKMKPLVVNELTSHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHI 360
           STGLKEDEIGRCIRKMKPLVVNELT+HLLSQMDRKCSICQEDYE DDEMGKLECGHSYHI
Sbjct: 301 STGLKEDEIGRCIRKMKPLVVNELTTHLLSQMDRKCSICQEDYEADDEMGKLECGHSYHI 360

Query: 361 HCIKQWLAQKNMCPVCKTAAGGRG 377
            CIKQWLAQKN CPVCKTAA GRG
Sbjct: 361 QCIKQWLAQKNTCPVCKTAAVGRG 375

[Close]

BLAST of Tan0006473 vs. TAIR 10
Match: AT1G73760.1 (RING/U-box superfamily protein )

HSP 1 Score: 295.0 bits (754), Expect = 8.3e-80
Identity = 177/389 (45.50%), Postives = 234/389 (60.15%), Query Frame = 0


Query: 2   PVLTHTSTVGEQIKLRRPKNHLSQPISESDPNPSIPSPQIPSIIQSTRCKSTISSLLLST 61
           P  + ++T+G+ ++L+RP+NH + P   +   P IP P       S   KS +SS  L  
Sbjct: 7   PSSSSSTTIGQHMRLQRPRNHRNLPPISTADEPLIPKP-------SRVSKSAMSSFFL-- 66

Query: 62  FSNNTTSGANESLPTNRKKNNFSSATFRGLGCTAAASQQVSVPAVIRTSADWQ----KKK 121
                       LP   KK    +A+FRGLGCT +ASQQVSVPAVIR+SADW     K K
Sbjct: 67  ------------LPETTKKKPNGTASFRGLGCTTSASQQVSVPAVIRSSADWDASNFKIK 126

Query: 122 TRKKKQKNSNNKTQQGIVDGS--HFQPNSNFNSASCLDAQDVWCGPGIGFSSDA--AASV 181
             KKK KN  + +  G   GS       S  +S +C    DVWCGPG+GFS+DA    S+
Sbjct: 127 KTKKKNKNKGSSSYNG---GSIKILSEASTSSSVACAAIPDVWCGPGVGFSTDAVVGGSI 186

Query: 182 DCVVA---RRHPSGRGKIDLEKMSQRER-------SCLGRRTVNPETLLFLDSDSDLPTA 241
           D VV+   RR+   R KID +K +           S L RR++N E+  + DSDS   T+
Sbjct: 187 DTVVSDPPRRNIPVRRKIDGDKTNSNSNNHREGSSSLLPRRSLNQESNPYFDSDSSFLTS 246

Query: 242 RSLEVSGSRYYRHVRHPSPDGLAEIMMFQSSLLMGGRFDIHDQFRELRLDVDNMSYEELL 301
           R+ +    RY+RH+R P PDGLAE+MM Q+  +MGG     DQFR++RL+VDNM+YE+LL
Sbjct: 247 RAEQT--DRYHRHLRLPYPDGLAEMMMMQNGFVMGGVLSSFDQFRDMRLNVDNMTYEQLL 306

Query: 302 ELGERIGHVSTGLKEDEIGRCIRKMKPLVVNELTSHLLSQMDRKCSICQEDYEPDDEMGK 361
           ELGERIGHV+TGL E +I  C+RK+KP   +   +      DRKC ICQ++YE  DE+G+
Sbjct: 307 ELGERIGHVNTGLTEKQIKSCLRKVKPCRQDTTVA------DRKCIICQDEYEAKDEVGE 363

Query: 362 LECGHSYHIHCIKQWLAQKNMCPVCKTAA 373
           L CGH +HI C+ QWL +KN CPVCKT A
Sbjct: 367 LRCGHRFHIDCVNQWLVRKNSCPVCKTMA 363

[Close]

BLAST of Tan0006473 vs. TAIR 10
Match: AT1G17970.1 (RING/U-box superfamily protein )

HSP 1 Score: 263.8 bits (673), Expect = 2.0e-70
Identity = 165/381 (43.31%), Postives = 229/381 (60.10%), Query Frame = 0


Query: 7   TSTVGEQIKLRRPKNHLSQPISESDPNPSIPSPQIPSIIQSTRCKSTISSLLLSTFSNNT 66
           ++T+GE I+LRR +N   + +  +D +P +    +P    +  C S +SS     F    
Sbjct: 3   STTIGEHIRLRRARNQTIRHLHAADDDPPLSHVVLPISQPNRFCNSAMSS-----FFPLP 62

Query: 67  TSGANESLPTNRKKNNFSSATFRGLGC---TAAASQQVSVPAVIRTSADWQKKKTRKKKQ 126
           TS +NES     +K  + +++FRG+GC    AAA+Q+VSVP+VIR SAD   +  + KK+
Sbjct: 63  TSSSNES----TRKKPYQTSSFRGMGCYAAAAAAAQEVSVPSVIRYSADLDARIRKDKKK 122

Query: 127 KNSNNKTQQGIVDGSHFQPNSNFNSASCLDAQDVWCGPGIGFSSDA--AASVDCVVARRH 186
           K   +K ++    GS+   +    S    D  DVWC PG+GFS+DA    SVD    R  
Sbjct: 123 KKHKHKKKKKKNKGSYEDGSIRILSEEARDVIDVWCRPGLGFSTDAVIGRSVDPPRGRNI 182

Query: 187 PSGRGKIDLEKMSQRE---RSCLGRRTVNPETLL--FLDSDSDLPTARSLE--VSGSRYY 246
           PS R KID++  +       S L  R +N ET      +SDS   T+   E  +  SR  
Sbjct: 183 PSSRRKIDVDNNNYNHTLGSSVLPIRFLNQETHSHDIFNSDSTFVTSSRAEPTMLSSRCR 242

Query: 247 RHVRHPSPDGLAEIMMFQSSLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHVST 306
            H+    PD L E+ M Q+  +MG   D  D + ELRLDVD+MSYE+LLELG+RIG+V+T
Sbjct: 243 GHLPRSYPDDLTEMRMLQNGFVMGRITDSRDNYHELRLDVDSMSYEQLLELGDRIGYVNT 302

Query: 307 GLKEDEIGRCIRKMKPLVVNELTSHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHC 366
           GLKE EI RC+ K+KP V     SH L  +DRKCSICQ++YE +DE+G+L CGHS+H+HC
Sbjct: 303 GLKESEIHRCLGKIKPSV-----SHTL--VDRKCSICQDEYEREDEVGELNCGHSFHVHC 362

Query: 367 IKQWLAQKNMCPVCKTAAGGR 376
           +KQWL++KN CPVCK AA G+
Sbjct: 363 VKQWLSRKNACPVCKKAAYGK 367

[Close]

BLAST of Tan0006473 vs. TAIR 10
Match: AT1G45180.1 (RING/U-box superfamily protein )

HSP 1 Score: 140.6 bits (353), Expect = 2.6e-33
Identity = 64/135 (47.41%), Postives = 92/135 (68.15%), Query Frame = 0


Query: 236 HVRHPSPDGLAEIMMFQSSLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHVSTG 295
           H+R      L ++M+   S+++G   DIHD++R++RLDVDNM+YEELL L ERIG V TG
Sbjct: 506 HMRRAGNLRLEDVMLLNQSIMLGAA-DIHDRYRDMRLDVDNMTYEELLSLEERIGDVCTG 565

Query: 296 LKEDEIGRCIRKMKPLVVNELTSHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCI 355
           L E+ I   +++ K       +S   SQ    C +CQE+Y+ ++E+G+LECGH +H  CI
Sbjct: 566 LNEETISNRLKQQK-----YKSSTRSSQEVEPCCVCQEEYKEEEEIGRLECGHDFHSQCI 625

Query: 356 KQWLAQKNMCPVCKT 371
           K+WL QKN+CP+CKT
Sbjct: 626 KEWLKQKNLCPICKT 634

[Close]

BLAST of Tan0006473 vs. TAIR 10
Match: AT4G31450.1 (RING/U-box superfamily protein )

HSP 1 Score: 137.5 bits (345), Expect = 2.2e-32
Identity = 64/131 (48.85%), Postives = 92/131 (70.23%), Query Frame = 0


Query: 247 EIMMFQSSLLMGGRFDIHDQFRELRLDVDNMSYEELLELGERIGHVSTGLKEDEIGRCIR 306
           ++++ ++ LL+GG    HDQ R++RLD+DNMSYEELL L ERIG VST L E+ I +C++
Sbjct: 363 DLLIMETGLLLGG-LSFHDQHRDMRLDIDNMSYEELLALEERIGTVSTALTEEAISKCLK 422

Query: 307 ----KMKPLVVNELT-SHLLSQMDRKCSICQEDYEPDDEMGKLECGHSYHIHCIKQWLAQ 366
               +MKPL    +T S   ++ D KCSICQE+Y   DE+G+L C H+YH+ C+++WL  
Sbjct: 423 TSIYQMKPLSYGSITKSPSDNKEDAKCSICQEEYTIGDEVGRLHCEHTYHVKCVQEWLRI 482

Query: 367 KNMCPVCKTAA 373
           K+ CP+CK  A
Sbjct: 483 KSWCPICKATA 492

[Close]

BLAST of Tan0006473 vs. TAIR 10
Match: AT2G15530.1 (RING/U-box superfamily protein )

HSP 1 Score: 132.9 bits (333), Expect = 5.4e-31
Identity = 69/149 (46.31%), Postives = 90/149 (60.40%), Query Frame = 0


Query: 224 RSLEVSGSRYYRHVRHPSPDGLAEIMMFQSSLLMGGRFDIHDQFRELRLDVDNMSYEELL 283
           R+  +S  R   H      +   E  M    L+  G  D+HD+ RE+RLDVDNMSYEELL
Sbjct: 553 RNQIISEIRQVLHAMRRGENLRVEDYMVFDPLIYQGMTDMHDRHREMRLDVDNMSYEELL 612

Query: 284 ELGERIGHVSTGLKEDEIGRCIRKMKPLVVNELTSHLLSQMDRKCSICQEDYEPDDEMGK 343
            LGERIG VSTGL E+ I + +++ K    +  +S  L Q    C ICQE+Y   D +G 
Sbjct: 613 ALGERIGDVSTGLSEEVILKAMKQHKH-TSSSPSSVELHQNIEPCCICQEEYVEGDNLGT 672

Query: 344 LECGHSYHIHCIKQWLAQKNMCPVCKTAA 373
           L+CGH +H  CIKQW+  KN+CP+CKT A
Sbjct: 673 LKCGHEFHKDCIKQWVMIKNLCPICKTEA 700

The following BLAST results are available for this feature:

BLAST of Tan0006473 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZQF9	7.7e-30	46.31	E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV...	[more]
O49500	2.5e-28	37.85	E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV...	[more]
Q9FMM4	2.5e-28	48.03	Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RH...	[more]
Q7XTV7	3.2e-28	49.59	Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=399...	[more]
Q5QLR5	1.3e-21	43.22	Probable E3 ubiquitin-protein ligase ZFP1 OS=Oryza sativa subsp. japonica OX=399...	[more]

BLAST of Tan0006473 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038894454.1	2.2e-191	90.08	E3 ubiquitin-protein ligase MBR2 isoform X2 [Benincasa hispida]	[more]
XP_038894453.1	5.4e-190	89.84	E3 ubiquitin-protein ligase MBR2 isoform X1 [Benincasa hispida]	[more]
XP_022137196.1	2.5e-187	89.47	uncharacterized protein LOC111008721 isoform X2 [Momordica charantia]	[more]
XP_022137195.1	6.2e-186	89.24	uncharacterized protein LOC111008721 isoform X1 [Momordica charantia]	[more]
XP_004137488.1	6.8e-185	87.76	E3 ubiquitin-protein ligase MBR2 isoform X2 [Cucumis sativus]	[more]

BLAST of Tan0006473 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1C5U0	1.2e-187	89.47	uncharacterized protein LOC111008721 isoform X2 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1C605	3.0e-186	89.24	uncharacterized protein LOC111008721 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A0A0LQF5	3.3e-185	87.76	RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G042600 P...	[more]
A0A6J1GUE7	3.1e-183	87.37	E3 ubiquitin-protein ligase MBR2-like isoform X2 OS=Cucurbita moschata OX=3662 G...	[more]
A0A5D3BIX1	6.9e-183	86.98	E3 ubiquitin-protein ligase MBR2 isoform X2 OS=Cucumis melo var. makuwa OX=11946...	[more]

BLAST of Tan0006473 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73760.1	8.3e-80	45.50	RING/U-box superfamily protein	[more]
AT1G17970.1	2.0e-70	43.31	RING/U-box superfamily protein	[more]
AT1G45180.1	2.6e-33	47.41	RING/U-box superfamily protein	[more]
AT4G31450.1	2.2e-32	48.85	RING/U-box superfamily protein	[more]
AT2G15530.1	5.4e-31	46.31	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 328..368 e-value: 2.7E-7 score: 40.3
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 327..369 e-value: 1.6E-12 score: 47.5
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 328..369 score: 12.636808
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 306..373 e-value: 7.4E-18 score: 65.6
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 127..141
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 111..141
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 17..42
None	No IPR available	PANTHER	PTHR22937:SF125	C3HC4-TYPE RING ZINC FINGER PROTEIN	coord: 6..370
None	No IPR available	PANTHER	PTHR22937	E3 UBIQUITIN-PROTEIN LIGASE RNF165	coord: 6..370
None	No IPR available	CDD	cd16481	RING-H2_TTC3	coord: 327..369 e-value: 1.08605E-16 score: 71.2241
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 318..371

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0006473.1	Tan0006473.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0061630	ubiquitin protein ligase activity