Tan0006414 (gene) Snake gourd v1

Overview
NameTan0006414
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionubiquitin-conjugating enzyme E2 20-like
LocationLG07: 12383953 .. 12385751 (-)
RNA-Seq ExpressionTan0006414
SyntenyTan0006414
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTCCTTCGTTTTAAAACCCTCCTTTCTCCTCTCTCCCTCTCTGCTCAGTCTCTCTCTCTCTCTCTCTCTCTCTACCATCGTCTAATTGATACTTCTTTGGTGGAGAAATCCTTGGATCATTCTCGAATCCTTCATATCCCCTCTGTAATTCGGATCAGAGAGAATGGCGGCCATTAATCGATACACTGACAACACGACGCCGGCCAACCCTGTTGTTTCTCCAGCCAAGAAACCCGTACCGCCGGCCAAAACCGCGGACTCTCAGTCCGTTCTCAAAAGGTATAAACATTTCCATATCTTTCGGATTTGATTCGATTCGTGCGTCGTCAGGGGATCGATCGCGTTTTTGTGCGTGTGCTATCGCCTCTGCTTTCTCGTGATTTTGGGCCATTATTGAACTTTATGCTGGATGAGTTTGTTTGTTGGTAGGGTTCCGGATTTAGTTTGAACTGATTTTGAGATGGGTTGTTTTGTGGTCTTTCGCAATCATCTTTTTAGGGTTTTATAGGGTAATTTGAGTGTCTTCTCAGGTTCCTCATTCTTGTGTATTTTTATGTAAAGAAATTAGTTAAGGACTCCGTCTGTATCCAACATAACTAACGATCTTCCTTCAATTTCTGTTGTGTTTTTCTGACTACAGGTTGCAATCTGAATTAATGGCTCTAATGGTGAGACTTGCTACATACATTTTTTTCATCTGATATTATTTTGGACTATTAAATCTTGGAGTATTTGGAAATGAGATTCCATAGTTGGCCAATAAACTCTTATAAATTCATGTGTATGATATTGCAGATGAGCGGAGACTCGGGAATCTCAGCCTTTCCTGAGGAGGATAACATTTTCTGCTGGAAAGGGACAATAACCGGAAGCAAAGACACAGTGTTTGAAGGAACAGAATATAGGTTATCCTTCTCATTCCCTAACGATTACCCTTTTAAGCCTCCAAAGGTGAAGTTTGAGACTGGCTGCTTTCACCCAAATGTGGACGTTTATGGCAATATTTGCTTGGACATACTTCAGGTAATTCAGCTATGGCGAAATCTTCGACATTGAATCAATAAATTCTCCGATTTTTCAGGTCGAAAACAACCCAAAATGAGTAATGTTTTATCTTTTGCAGGATAAATGGTCATCAGCATATGATGTGAGAACAATACTTCTATCCATCCAGAGTCTGCTTGGAGGTAACCTCTCAAAAACACCATACTTCCTTGACTTAGCAGATGGTTAAGAATGGAATATAGAATTTGCAACAAGTTGTTGCCATTTATTTGCAGAACCAAACATAAGTTCACCATTAAACACCCAAGCAGCTCAATTGTGGAGCAATCAAGAAGGTGGGTTTCATTATTAACACATAATTTTTCTTCTCTCTTTGAGTTGTTGGCCATGTGATGGAAAATTGGCTCACTCATCATGTGTTTCTGTGGTTGTTGCTGTAGAATATCGGAAAATGGTGGAGAAATTGTACAAGCCTCCAACAGCAGCAGCTTAGCTTGTTTTAGTTTACGCGAAGAAGAAACTAGGAGGGAGGATTTTGTGTCTCTCACTCTTTTTGCCCTAGAGAGGAGATTCCCAAATGATTTTTTTTTTTTTTTTTTCAGTGTGCTCTTCATTTCTTTTACTCTCTGGCTGGATGTTACAGATATATTTGTTGTCTCCTGTTATATGTGGAAATTTCAAGCCATGAGCTATATTTGTTCTCATTTCAAAGTTCCTCTATCATATCCTGCCTTGCTCTTTTTTTGAAGGTTTTTGAAATGGAAATTATGCTCAAAATTCTCATGTATTCAAG

mRNA sequence

TCTCCTTCGTTTTAAAACCCTCCTTTCTCCTCTCTCCCTCTCTGCTCAGTCTCTCTCTCTCTCTCTCTCTCTCTACCATCGTCTAATTGATACTTCTTTGGTGGAGAAATCCTTGGATCATTCTCGAATCCTTCATATCCCCTCTGTAATTCGGATCAGAGAGAATGGCGGCCATTAATCGATACACTGACAACACGACGCCGGCCAACCCTGTTGTTTCTCCAGCCAAGAAACCCGTACCGCCGGCCAAAACCGCGGACTCTCAGTCCGTTCTCAAAAGGTTGCAATCTGAATTAATGGCTCTAATGATGAGCGGAGACTCGGGAATCTCAGCCTTTCCTGAGGAGGATAACATTTTCTGCTGGAAAGGGACAATAACCGGAAGCAAAGACACAGTGTTTGAAGGAACAGAATATAGGTTATCCTTCTCATTCCCTAACGATTACCCTTTTAAGCCTCCAAAGGTGAAGTTTGAGACTGGCTGCTTTCACCCAAATGTGGACGTTTATGGCAATATTTGCTTGGACATACTTCAGGATAAATGGTCATCAGCATATGATGTGAGAACAATACTTCTATCCATCCAGAGTCTGCTTGGAGAACCAAACATAAGTTCACCATTAAACACCCAAGCAGCTCAATTGTGGAGCAATCAAGAAGAATATCGGAAAATGGTGGAGAAATTGTACAAGCCTCCAACAGCAGCAGCTTAGCTTGTTTTAGTTTACGCGAAGAAGAAACTAGGAGGGAGGATTTTGTGTCTCTCACTCTTTTTGCCCTAGAGAGGAGATTCCCAAATGATTTTTTTTTTTTTTTTTTCAGTGTGCTCTTCATTTCTTTTACTCTCTGGCTGGATGTTACAGATATATTTGTTGTCTCCTGTTATATGTGGAAATTTCAAGCCATGAGCTATATTTGTTCTCATTTCAAAGTTCCTCTATCATATCCTGCCTTGCTCTTTTTTTGAAGGTTTTTGAAATGGAAATTATGCTCAAAATTCTCATGTATTCAAG

Coding sequence (CDS)

ATGGCGGCCATTAATCGATACACTGACAACACGACGCCGGCCAACCCTGTTGTTTCTCCAGCCAAGAAACCCGTACCGCCGGCCAAAACCGCGGACTCTCAGTCCGTTCTCAAAAGGTTGCAATCTGAATTAATGGCTCTAATGATGAGCGGAGACTCGGGAATCTCAGCCTTTCCTGAGGAGGATAACATTTTCTGCTGGAAAGGGACAATAACCGGAAGCAAAGACACAGTGTTTGAAGGAACAGAATATAGGTTATCCTTCTCATTCCCTAACGATTACCCTTTTAAGCCTCCAAAGGTGAAGTTTGAGACTGGCTGCTTTCACCCAAATGTGGACGTTTATGGCAATATTTGCTTGGACATACTTCAGGATAAATGGTCATCAGCATATGATGTGAGAACAATACTTCTATCCATCCAGAGTCTGCTTGGAGAACCAAACATAAGTTCACCATTAAACACCCAAGCAGCTCAATTGTGGAGCAATCAAGAAGAATATCGGAAAATGGTGGAGAAATTGTACAAGCCTCCAACAGCAGCAGCTTAG

Protein sequence

MAAINRYTDNTTPANPVVSPAKKPVPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPEEDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTAAA
Homology
[Close]
BLAST of Tan0006414 vs. ExPASy Swiss-Prot
Match: Q8L7T3 (Ubiquitin-conjugating enzyme E2 20 OS=Arabidopsis thaliana OX=3702 GN=UBC20 PE=2 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 1.2e-84
Identity = 154/180 (85.56%), Postives = 159/180 (88.33%), Query Frame = 0 

Query: 1   MAAINRYTDNTTPANPVVSPAKKPVPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAA+N Y  NT    P  + +K+P  P KT DSQSVLKRLQSELM LMM G  GISAFPE
Sbjct: 1   MAAVNGYQGNTPADPPASNGSKQPAAPTKTVDSQSVLKRLQSELMGLMMGGGPGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKDTVFEGTEYRLS SF NDYPFKPPKVKFET CFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSLSFSNDYPFKPPKVKFETCCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPP+A
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPSA 180

[Close]
BLAST of Tan0006414 vs. ExPASy Swiss-Prot
Match: Q9LJZ5 (Ubiquitin-conjugating enzyme E2 19 OS=Arabidopsis thaliana OX=3702 GN=UBC19 PE=1 SV=1)

HSP 1 Score: 300.8 bits (769), Expect = 1.0e-80
Identity = 149/181 (82.32%), Postives = 155/181 (85.64%), Query Frame = 0 

Query: 1   MAAINRYTDNTTPA-NPVVSPAKKPVPPAKTADSQSVLKRLQSELMALMMSGDSGISAFP 60
           MA +N YT NT  A  P  + +K+  PP KT DS SVLKRLQSELM LMM  D GISAFP
Sbjct: 1   MATVNGYTGNTPAATTPAATGSKQSAPPTKTVDSHSVLKRLQSELMGLMMGADPGISAFP 60

Query: 61  EEDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNIC 120
           EEDNIFCWKGTITGSKDTVFEGTEYRLS +F NDYPFK PKVKFET CFHPNVD+YGNIC
Sbjct: 61  EEDNIFCWKGTITGSKDTVFEGTEYRLSLTFSNDYPFKSPKVKFETCCFHPNVDLYGNIC 120

Query: 121 LDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPT 180
           LDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLN QAAQLWSNQEEYRKMVEKLYKP  
Sbjct: 121 LDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNNQAAQLWSNQEEYRKMVEKLYKPLN 180

[Close]
BLAST of Tan0006414 vs. ExPASy Swiss-Prot
Match: Q54XS6 (Probable ubiquitin-conjugating enzyme E2 C OS=Dictyostelium discoideum OX=44689 GN=ube2c PE=3 SV=1)

HSP 1 Score: 209.9 bits (533), Expect = 2.4e-53
Identity = 98/150 (65.33%), Postives = 119/150 (79.33%), Query Frame = 0 

Query: 31  ADSQSVLKRLQSELMALMMSGDSGISAFPEEDNIFCWKGTITGSKDTVFEGTEYRLSFSF 90
           +   SV KRLQSELM LMM    GISAFP+ DNIF W GTI G+KDTV+E  E+++S  F
Sbjct: 2   SQDHSVSKRLQSELMNLMMGPSPGISAFPDGDNIFNWIGTIQGAKDTVYEQMEFKMSLKF 61

Query: 91  PNDYPFKPPKVKFETGCFHPNVDVYGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNIS 150
           P DYP+KPP VKFET CFHPNVD  GNICLDIL+DKWS  Y+VR++L+SIQSLLGEPN  
Sbjct: 62  PTDYPYKPPTVKFETQCFHPNVDNNGNICLDILKDKWSPVYNVRSLLISIQSLLGEPNNE 121

Query: 151 SPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 181
           SPLN+ AA LWSNQ+EY+K+++K Y+  T+
Sbjct: 122 SPLNSYAASLWSNQDEYKKVLDKRYQEATS 151

[Close]
BLAST of Tan0006414 vs. ExPASy Swiss-Prot
Match: Q4R9D1 (Ubiquitin-conjugating enzyme E2 C OS=Macaca fascicularis OX=9541 GN=UBE2C PE=2 SV=1)

HSP 1 Score: 205.3 bits (521), Expect = 5.9e-52
Identity = 101/168 (60.12%), Postives = 120/168 (71.43%), Query Frame = 0 

Query: 8   TDNTTPANPVVSPAKKPVPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPEEDNIFCW 67
           + N  PA   V+ A+K   P+  A    V KRLQ ELM LMMSGD GISAFPE DN+F W
Sbjct: 3   SQNRDPAATSVTAARKGAEPSGGAARGPVGKRLQQELMTLMMSGDKGISAFPESDNLFKW 62

Query: 68  KGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICLDILQDKW 127
            GTI G+  TV+E   Y+LS  FP+ YP+  P VKF T C+HPNVD  GNICLDIL+DKW
Sbjct: 63  VGTIHGAAGTVYEDLRYKLSLEFPSGYPYNAPTVKFLTPCYHPNVDTQGNICLDILKDKW 122

Query: 128 SSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLY 176
           S+ YDVRTILLSIQSLLGEPNI SPLNT AA+LW N   ++K +++ Y
Sbjct: 123 SALYDVRTILLSIQSLLGEPNIDSPLNTHAAELWKNPTAFKKYLQETY 170

[Close]
BLAST of Tan0006414 vs. ExPASy Swiss-Prot
Match: Q95044 (Ubiquitin-conjugating enzyme E2 C OS=Spisula solidissima OX=6584 GN=UBE2C PE=1 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 1.3e-51
Identity = 96/167 (57.49%), Postives = 124/167 (74.25%), Query Frame = 0 

Query: 10  NTTPANPVVSPAKKPVPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPEEDNIFCWKG 69
           N  PA   V   ++P     + +  SV KRLQ EL  L+MSGD GI+AFP+ DN+F W  
Sbjct: 5   NIDPAANQVRQKERPRDMTTSKERHSVSKRLQQELRTLLMSGDPGITAFPDGDNLFKWVA 64

Query: 70  TITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICLDILQDKWSS 129
           T+ G KDTV+E  +Y+L+  FP+DYP+KPP VKF T C+HPNVD  GNICLDIL++ W++
Sbjct: 65  TLDGPKDTVYESLKYKLTLEFPSDYPYKPPVVKFTTPCWHPNVDQSGNICLDILKENWTA 124

Query: 130 AYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYK 177
           +YDVRTILLS+QSLLGEPN +SPLN QAA +WSNQ EY+K++ + YK
Sbjct: 125 SYDVRTILLSLQSLLGEPNNASPLNAQAADMWSNQTEYKKVLHEKYK 171

[Close]
BLAST of Tan0006414 vs. NCBI nr
Match: XP_038900928.1 (LOW QUALITY PROTEIN: ubiquitin-conjugating enzyme E2 20 [Benincasa hispida])

HSP 1 Score: 364.0 bits (933), Expect = 7.5e-97
Identity = 177/181 (97.79%), Postives = 180/181 (99.45%), Query Frame = 0 

Query: 1   MAAINRYTDNTTPANPVVSPAKKPVPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNTTPANPVVSPAK+ +PPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSK+TVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180

Query: 181 A 182
           A
Sbjct: 181 A 181

[Close]
BLAST of Tan0006414 vs. NCBI nr
Match: XP_022957735.1 (ubiquitin-conjugating enzyme E2 20-like [Cucurbita moschata] >KAG6605929.1 Ubiquitin-conjugating enzyme E2 19, partial [Cucurbita argyrosperma subsp. sororia] >KAG7035878.1 Ubiquitin-conjugating enzyme E2 19, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 364.0 bits (933), Expect = 7.5e-97
Identity = 177/182 (97.25%), Postives = 180/182 (98.90%), Query Frame = 0 

Query: 1   MAAINRYTDNTTPANPVVSPAKKPVPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNTTPANPVVSPAK+ +PPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPN SSPLNTQAAQLWSNQE+YRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA 180

Query: 181 AA 183
           AA
Sbjct: 181 AA 182

[Close]
BLAST of Tan0006414 vs. NCBI nr
Match: XP_023533942.1 (ubiquitin-conjugating enzyme E2 20-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 363.6 bits (932), Expect = 9.8e-97
Identity = 176/182 (96.70%), Postives = 180/182 (98.90%), Query Frame = 0 

Query: 1   MAAINRYTDNTTPANPVVSPAKKPVPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNTTPANPVVSPAK+ +PPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVD+YGNICL
Sbjct: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDIYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPN SSPLNTQAAQLWSNQE+YRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA 180

Query: 181 AA 183
           AA
Sbjct: 181 AA 182

[Close]
BLAST of Tan0006414 vs. NCBI nr
Match: XP_004140207.1 (ubiquitin-conjugating enzyme E2 20 [Cucumis sativus] >XP_008449688.1 PREDICTED: ubiquitin-conjugating enzyme E2 20 [Cucumis melo] >KAA0061782.1 ubiquitin-conjugating enzyme E2 20-like isoform X3 [Cucumis melo var. makuwa] >KGN48113.1 hypothetical protein Csa_003824 [Cucumis sativus] >TYJ96123.1 ubiquitin-conjugating enzyme E2 20-like isoform X3 [Cucumis melo var. makuwa])

HSP 1 Score: 362.1 bits (928), Expect = 2.9e-96
Identity = 176/181 (97.24%), Postives = 179/181 (98.90%), Query Frame = 0 

Query: 1   MAAINRYTDNTTPANPVVSPAKKPVPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNT PANPVVSPAK+ +PPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSK+TVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180

Query: 181 A 182
           A
Sbjct: 181 A 181

[Close]
BLAST of Tan0006414 vs. NCBI nr
Match: XP_022943408.1 (ubiquitin-conjugating enzyme E2 20-like [Cucurbita moschata] >XP_022986183.1 ubiquitin-conjugating enzyme E2 20-like [Cucurbita maxima] >XP_023513276.1 ubiquitin-conjugating enzyme E2 20-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 361.7 bits (927), Expect = 3.7e-96
Identity = 176/181 (97.24%), Postives = 179/181 (98.90%), Query Frame = 0 

Query: 1   MAAINRYTDNTTPANPVVSPAKKPVPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINR+TDNTTPAN VVSPAKK +PPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRHTDNTTPANAVVSPAKKSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVD+YGNICL
Sbjct: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDIYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180

Query: 181 A 182
           A
Sbjct: 181 A 181

[Close]
BLAST of Tan0006414 vs. ExPASy TrEMBL
Match: A0A6J1H1C8 (ubiquitin-conjugating enzyme E2 20-like OS=Cucurbita moschata OX=3662 GN=LOC111459191 PE=3 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 3.6e-97
Identity = 177/182 (97.25%), Postives = 180/182 (98.90%), Query Frame = 0 

Query: 1   MAAINRYTDNTTPANPVVSPAKKPVPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNTTPANPVVSPAK+ +PPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTTPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPN SSPLNTQAAQLWSNQE+YRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNTSSPLNTQAAQLWSNQEDYRKMVEKLYKPPTA 180

Query: 181 AA 183
           AA
Sbjct: 181 AA 182

[Close]
BLAST of Tan0006414 vs. ExPASy TrEMBL
Match: A0A5D3BA03 (Ubiquitin-conjugating enzyme E2 20-like isoform X3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold182G00870 PE=3 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 1.4e-96
Identity = 176/181 (97.24%), Postives = 179/181 (98.90%), Query Frame = 0 

Query: 1   MAAINRYTDNTTPANPVVSPAKKPVPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNT PANPVVSPAK+ +PPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSK+TVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180

Query: 181 A 182
           A
Sbjct: 181 A 181

[Close]
BLAST of Tan0006414 vs. ExPASy TrEMBL
Match: A0A0A0KF07 (UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G434330 PE=3 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 1.4e-96
Identity = 176/181 (97.24%), Postives = 179/181 (98.90%), Query Frame = 0 

Query: 1   MAAINRYTDNTTPANPVVSPAKKPVPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNT PANPVVSPAK+ +PPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSK+TVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180

Query: 181 A 182
           A
Sbjct: 181 A 181

[Close]
BLAST of Tan0006414 vs. ExPASy TrEMBL
Match: A0A1S3BLY8 (ubiquitin-conjugating enzyme E2 20 OS=Cucumis melo OX=3656 GN=LOC103491487 PE=3 SV=1)

HSP 1 Score: 362.1 bits (928), Expect = 1.4e-96
Identity = 176/181 (97.24%), Postives = 179/181 (98.90%), Query Frame = 0 

Query: 1   MAAINRYTDNTTPANPVVSPAKKPVPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINRYTDNT PANPVVSPAK+ +PPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRYTDNTAPANPVVSPAKQSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSK+TVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKETVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180

Query: 181 A 182
           A
Sbjct: 181 A 181

[Close]
BLAST of Tan0006414 vs. ExPASy TrEMBL
Match: A0A6J1JFS1 (ubiquitin-conjugating enzyme E2 20-like OS=Cucurbita maxima OX=3661 GN=LOC111484001 PE=3 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 1.8e-96
Identity = 176/181 (97.24%), Postives = 179/181 (98.90%), Query Frame = 0 

Query: 1   MAAINRYTDNTTPANPVVSPAKKPVPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAAINR+TDNTTPAN VVSPAKK +PPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE
Sbjct: 1   MAAINRHTDNTTPANAVVSPAKKSLPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVD+YGNICL
Sbjct: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDIYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180

Query: 181 A 182
           A
Sbjct: 181 A 181

[Close]
BLAST of Tan0006414 vs. TAIR 10
Match: AT1G50490.1 (ubiquitin-conjugating enzyme 20 )

HSP 1 Score: 313.9 bits (803), Expect = 8.3e-86
Identity = 154/180 (85.56%), Postives = 159/180 (88.33%), Query Frame = 0 

Query: 1   MAAINRYTDNTTPANPVVSPAKKPVPPAKTADSQSVLKRLQSELMALMMSGDSGISAFPE 60
           MAA+N Y  NT    P  + +K+P  P KT DSQSVLKRLQSELM LMM G  GISAFPE
Sbjct: 1   MAAVNGYQGNTPADPPASNGSKQPAAPTKTVDSQSVLKRLQSELMGLMMGGGPGISAFPE 60

Query: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNICL 120
           EDNIFCWKGTITGSKDTVFEGTEYRLS SF NDYPFKPPKVKFET CFHPNVDVYGNICL
Sbjct: 61  EDNIFCWKGTITGSKDTVFEGTEYRLSLSFSNDYPFKPPKVKFETCCFHPNVDVYGNICL 120

Query: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPTA 180
           DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPP+A
Sbjct: 121 DILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPSA 180

[Close]
BLAST of Tan0006414 vs. TAIR 10
Match: AT3G20060.1 (ubiquitin-conjugating enzyme19 )

HSP 1 Score: 300.8 bits (769), Expect = 7.3e-82
Identity = 149/181 (82.32%), Postives = 155/181 (85.64%), Query Frame = 0 

Query: 1   MAAINRYTDNTTPA-NPVVSPAKKPVPPAKTADSQSVLKRLQSELMALMMSGDSGISAFP 60
           MA +N YT NT  A  P  + +K+  PP KT DS SVLKRLQSELM LMM  D GISAFP
Sbjct: 1   MATVNGYTGNTPAATTPAATGSKQSAPPTKTVDSHSVLKRLQSELMGLMMGADPGISAFP 60

Query: 61  EEDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFETGCFHPNVDVYGNIC 120
           EEDNIFCWKGTITGSKDTVFEGTEYRLS +F NDYPFK PKVKFET CFHPNVD+YGNIC
Sbjct: 61  EEDNIFCWKGTITGSKDTVFEGTEYRLSLTFSNDYPFKSPKVKFETCCFHPNVDLYGNIC 120

Query: 121 LDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQEEYRKMVEKLYKPPT 180
           LDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLN QAAQLWSNQEEYRKMVEKLYKP  
Sbjct: 121 LDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNNQAAQLWSNQEEYRKMVEKLYKPLN 180

[Close]
BLAST of Tan0006414 vs. TAIR 10
Match: AT3G20060.2 (ubiquitin-conjugating enzyme19 )

HSP 1 Score: 258.8 bits (660), Expect = 3.2e-69
Identity = 123/136 (90.44%), Postives = 125/136 (91.91%), Query Frame = 0 

Query: 45  MALMMSGDSGISAFPEEDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFKPPKVKFE 104
           M LMM  D GISAFPEEDNIFCWKGTITGSKDTVFEGTEYRLS +F NDYPFK PKVKFE
Sbjct: 1   MGLMMGADPGISAFPEEDNIFCWKGTITGSKDTVFEGTEYRLSLTFSNDYPFKSPKVKFE 60

Query: 105 TGCFHPNVDVYGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQAAQLWSNQ 164
           T CFHPNVD+YGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLN QAAQLWSNQ
Sbjct: 61  TCCFHPNVDLYGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNNQAAQLWSNQ 120

Query: 165 EEYRKMVEKLYKPPTA 181
           EEYRKMVEKLYKP  A
Sbjct: 121 EEYRKMVEKLYKPLNA 136

[Close]
BLAST of Tan0006414 vs. TAIR 10
Match: AT2G02760.1 (ubiquiting-conjugating enzyme 2 )

HSP 1 Score: 131.3 bits (329), Expect = 7.7e-31
Identity = 63/138 (45.65%), Postives = 90/138 (65.22%), Query Frame = 0 

Query: 38  KRLQSELMALMMSGDSGISAFPEEDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFK 97
           KRL  +   L     +GIS  P+++NI  W   I G  DT ++G  ++LS  F  DYP K
Sbjct: 7   KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLSLQFSEDYPNK 66

Query: 98  PPKVKFETGCFHPNVDVYGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQA 157
           PP V+F +  FHPN+   G+ICLDILQ++WS  YDV  IL SIQSLL +PN +SP N++A
Sbjct: 67  PPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEA 126

Query: 158 AQLWS-NQEEYRKMVEKL 175
           A+++S ++ EY + V ++
Sbjct: 127 ARMFSESKREYNRRVREV 144

[Close]
BLAST of Tan0006414 vs. TAIR 10
Match: AT1G14400.1 (ubiquitin carrier protein 1 )

HSP 1 Score: 131.0 bits (328), Expect = 1.0e-30
Identity = 63/135 (46.67%), Postives = 88/135 (65.19%), Query Frame = 0 

Query: 38  KRLQSELMALMMSGDSGISAFPEEDNIFCWKGTITGSKDTVFEGTEYRLSFSFPNDYPFK 97
           KRL  +   L     +GIS  P+++NI  W   I G  DT ++G  ++LS  F  DYP K
Sbjct: 7   KRLMRDFKRLQQDPPAGISGAPQDNNIMLWNAVIFGPDDTPWDGGTFKLSLQFSEDYPNK 66

Query: 98  PPKVKFETGCFHPNVDVYGNICLDILQDKWSSAYDVRTILLSIQSLLGEPNISSPLNTQA 157
           PP V+F +  FHPN+   G+ICLDILQ++WS  YDV  IL SIQSLL +PN +SP N++A
Sbjct: 67  PPTVRFVSRMFHPNIYADGSICLDILQNQWSPIYDVAAILTSIQSLLCDPNPNSPANSEA 126

Query: 158 AQLWS-NQEEYRKMV 172
           A+++S ++ EY + V
Sbjct: 127 ARMYSESKREYNRRV 141

The following BLAST results are available for this feature:
BLAST of Tan0006414 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8L7T31.2e-8485.56Ubiquitin-conjugating enzyme E2 20 OS=Arabidopsis thaliana OX=3702 GN=UBC20 PE=2... [more]
Q9LJZ51.0e-8082.32Ubiquitin-conjugating enzyme E2 19 OS=Arabidopsis thaliana OX=3702 GN=UBC19 PE=1... [more]
Q54XS62.4e-5365.33Probable ubiquitin-conjugating enzyme E2 C OS=Dictyostelium discoideum OX=44689 ... [more]
Q4R9D15.9e-5260.12Ubiquitin-conjugating enzyme E2 C OS=Macaca fascicularis OX=9541 GN=UBE2C PE=2 S... [more]
Q950441.3e-5157.49Ubiquitin-conjugating enzyme E2 C OS=Spisula solidissima OX=6584 GN=UBE2C PE=1 S... [more]
BLAST of Tan0006414 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038900928.17.5e-9797.79LOW QUALITY PROTEIN: ubiquitin-conjugating enzyme E2 20 [Benincasa hispida][more]
XP_022957735.17.5e-9797.25ubiquitin-conjugating enzyme E2 20-like [Cucurbita moschata] >KAG6605929.1 Ubiqu... [more]
XP_023533942.19.8e-9796.70ubiquitin-conjugating enzyme E2 20-like [Cucurbita pepo subsp. pepo][more]
XP_004140207.12.9e-9697.24ubiquitin-conjugating enzyme E2 20 [Cucumis sativus] >XP_008449688.1 PREDICTED: ... [more]
XP_022943408.13.7e-9697.24ubiquitin-conjugating enzyme E2 20-like [Cucurbita moschata] >XP_022986183.1 ubi... [more]
BLAST of Tan0006414 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1H1C83.6e-9797.25ubiquitin-conjugating enzyme E2 20-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A5D3BA031.4e-9697.24Ubiquitin-conjugating enzyme E2 20-like isoform X3 OS=Cucumis melo var. makuwa O... [more]
A0A0A0KF071.4e-9697.24UBC core domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G434330 PE... [more]
A0A1S3BLY81.4e-9697.24ubiquitin-conjugating enzyme E2 20 OS=Cucumis melo OX=3656 GN=LOC103491487 PE=3 ... [more]
A0A6J1JFS11.8e-9697.24ubiquitin-conjugating enzyme E2 20-like OS=Cucurbita maxima OX=3661 GN=LOC111484... [more]
BLAST of Tan0006414 vs. TAIR 10
Match NameE-valueIdentityDescription
AT1G50490.18.3e-8685.56ubiquitin-conjugating enzyme 20 [more]
AT3G20060.17.3e-8282.32ubiquitin-conjugating enzyme19 [more]
AT3G20060.23.2e-6990.44ubiquitin-conjugating enzyme19 [more]
AT2G02760.17.7e-3145.65ubiquiting-conjugating enzyme 2 [more]
AT1G14400.11.0e-3046.67ubiquitin carrier protein 1 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableSMARTSM00212ubc_7coord: 38..133
e-value: 6.8E-15
score: 65.5
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..34
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..17
NoneNo IPR availablePANTHERPTHR24068UBIQUITIN-CONJUGATING ENZYME E2coord: 19..124
NoneNo IPR availablePANTHERPTHR24068:SF228UBIQUITIN-CONJUGATING ENZYME E2 20coord: 19..124
IPR016135Ubiquitin-conjugating enzyme/RWD-likeGENE3D3.10.110.10Ubiquitin Conjugating Enzymecoord: 4..131
e-value: 3.6E-36
score: 126.8
IPR016135Ubiquitin-conjugating enzyme/RWD-likeSUPERFAMILY54495UBC-likecoord: 29..130
IPR000608Ubiquitin-conjugating enzyme E2PFAMPF00179UQ_concoord: 39..129
e-value: 2.2E-29
score: 101.9
IPR000608Ubiquitin-conjugating enzyme E2PROSITEPS50127UBC_2coord: 35..134
score: 34.166981
IPR000608Ubiquitin-conjugating enzyme E2CDDcd00195UBCccoord: 38..123
e-value: 5.98299E-39
score: 126.163
IPR023313Ubiquitin-conjugating enzyme, active sitePROSITEPS00183UBC_1coord: 108..123

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0006414.1Tan0006414.1mRNA
Tan0006414.2Tan0006414.2mRNA
Tan0006414.3Tan0006414.3mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0031145 anaphase-promoting complex-dependent catabolic process
biological_process GO:0000209 protein polyubiquitination
biological_process GO:0030071 regulation of mitotic metaphase/anaphase transition
cellular_component GO:0005634 nucleus
molecular_function GO:0005524 ATP binding
molecular_function GO:0061631 ubiquitin conjugating enzyme activity