Tan0006333 (gene) Snake gourd v1

BLAST of Tan0006333 vs. ExPASy Swiss-Prot
Match: Q9SG12 (CDPK-related kinase 6 OS=Arabidopsis thaliana OX=3702 GN=CRK6 PE=1 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 3.4e-218
Identity = 383/592 (64.70%), Postives = 472/592 (79.73%), Query Frame = 0

Query: 1   MGHFCSKSVSDVNNDTAISGGTRPRSSHSGASPLPSGDAYSRPEESRQSYSVSTTASPFS 60
           MGH  S+++S V++D  I   T      S  +   +  + S P+    S       SPF 
Sbjct: 1   MGHCYSRNISTVDDDDEIPSATAQLPHRSHQNHHQTSSSSSIPQSPATSEVNPYNISPFQ 60

Query: 61  SPLPAGIAPSPARTPGRKFRWPLPPPSPAKPIMAILRRK------SRKASIPEDNQFGEE 120
           SPLPAG+APSPARTPGRKF+WP PPPSPAKPIMA LRR+       R   IPED++ G  
Sbjct: 61  SPLPAGVAPSPARTPGRKFKWPFPPPSPAKPIMAALRRRRGTAPHPRDGPIPEDSEAGGS 120

Query: 121 --EVGEIQLDKSFGYSKNFGAKFELGKEIGRGHFGHTFWAKGKRGDLKGKPVAVKIISKS 180
              +GE +LDK+FG++KNF  K+ELG+E+GRGHFGHT WAK K+G +KG+ VAVKIISKS
Sbjct: 121 GGGIGE-RLDKNFGFAKNFEGKYELGREVGRGHFGHTCWAKAKKGKIKGQTVAVKIISKS 180

Query: 181 KMTSAVGIEDVRREVKILKALSGHKNLVQFHDAFEDTNNIYMVMELCEGGELLERIVSRG 240
           KMTSA+ IEDVRREVK+LKALSGH ++V+F+D FED++N+++VMELCEGGELL+ I++RG
Sbjct: 181 KMTSALSIEDVRREVKLLKALSGHSHMVKFYDVFEDSDNVFVVMELCEGGELLDSILARG 240

Query: 241 GRYPEQESKTIVVQILSVAAFCHLQGVVHRDLKPENFLFVNKDENTGLKVIDFGLSDFVK 300
           GRYPE E+K I+VQILS  AF HLQGVVHRDLKPENFLF +K+E+  LKVIDFGLSD+ +
Sbjct: 241 GRYPEAEAKRILVQILSATAFFHLQGVVHRDLKPENFLFTSKNEDAVLKVIDFGLSDYAR 300

Query: 301 PDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGGRPFWARTESGIFRSVL 360
            D+RLNDVVGSAYYVAPEVL+RSYS EAD+WSIGVI+YILLCG RPF+ RTES IFR VL
Sbjct: 301 FDQRLNDVVGSAYYVAPEVLHRSYSTEADIWSIGVISYILLCGSRPFYGRTESAIFRCVL 360

Query: 361 RADPNFDDSPWPTISAEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDESLAVPLDNFIYK 420
           RA+PNFDD PWP+IS  AKDFVKRLLNKDHRKRMTAAQAL HPWLRDE+  + LD  IYK
Sbjct: 361 RANPNFDDLPWPSISPIAKDFVKRLLNKDHRKRMTAAQALAHPWLRDENPGLLLDFSIYK 420

Query: 421 SVKAYLRATPFKRAALKALAKALTEDELFYLRTQFKLLEPQNGFVALDNFKAALVRNATD 480
            VK+Y+RA+PF+RAALK+L+KA+ E+EL +L+ QF LLEP++G + L NF  AL R ATD
Sbjct: 421 LVKSYIRASPFRRAALKSLSKAIPEEELVFLKAQFMLLEPEDGGLHLHNFTTALTRYATD 480

Query: 481 AMRESNVADILKMMESLAYKRMDFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNR 540
           AM ES + DIL MM+ LA+K++DFEEFCAA++SVYQLEA+  WE IAT AFE+FE EG+R
Sbjct: 481 AMIESRLPDILNMMQPLAHKKLDFEEFCAASVSVYQLEALEEWEQIATVAFEHFESEGSR 540

Query: 541 VISVHELVQEMNLGPAAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRNSNTR 585
            ISV EL +EM+LGP A+  L++W RS DGKL+F GY +FLHGVT+R+S++R
Sbjct: 541 AISVQELAEEMSLGPNAYPLLKDWIRSLDGKLNFLGYAKFLHGVTVRSSSSR 591

BLAST of Tan0006333 vs. ExPASy Swiss-Prot
Match: Q9FIM9 (CDPK-related kinase 4 OS=Arabidopsis thaliana OX=3702 GN=CRK4 PE=1 SV=1)

HSP 1 Score: 756.9 bits (1953), Expect = 1.7e-217
Identity = 382/598 (63.88%), Postives = 477/598 (79.77%), Query Frame = 0

Query: 1   MGHFCSKSVSDVNNDTAISG----GTRPRSSHSGASPLPSGDAYSRPEESRQSYSVSTTA 60
           MGH  S+++S V +D   +G      +P  +H  AS   S  A   PE +  SY++    
Sbjct: 1   MGHCYSRNISAVEDDEIPTGNGEVSNQPSQNHRHASIPQSPVASGTPEVN--SYNI---- 60

Query: 61  SPFSSPLPAGIAPSPARTPGRKFRWPLPPPSPAKPIMAILRRK------SRKASIPEDNQ 120
           SPF SPLPAG+APSPARTPGRKF+WP PPPSPAKPIMA LRR+       R   IPED++
Sbjct: 61  SPFQSPLPAGVAPSPARTPGRKFKWPFPPPSPAKPIMAALRRRRGAPPQPRDEPIPEDSE 120

Query: 121 ----FGEEEVGEIQLDKSFGYSKNFGAKFELGKEIGRGHFGHTFWAKGKRGDLKGKPVAV 180
                G +  G  +LDK+FG+ KNF  K+ELGKE+GRGHFGHT WAK K+G +K + VAV
Sbjct: 121 DVVDHGGDSGGGERLDKNFGFGKNFEGKYELGKEVGRGHFGHTCWAKAKKGKMKNQTVAV 180

Query: 181 KIISKSKMTSAVGIEDVRREVKILKALSGHKNLVQFHDAFEDTNNIYMVMELCEGGELLE 240
           KIISK+KMTS + IEDVRREVK+LKALSGH+++V+F+D +ED +N+++VMELCEGGELL+
Sbjct: 181 KIISKAKMTSTLSIEDVRREVKLLKALSGHRHMVKFYDVYEDADNVFVVMELCEGGELLD 240

Query: 241 RIVSRGGRYPEQESKTIVVQILSVAAFCHLQGVVHRDLKPENFLFVNKDENTGLKVIDFG 300
           RI++RGGRYPE ++K I+VQILS  AF HLQGVVHRDLKPENFLF +++E+  LKVIDFG
Sbjct: 241 RILARGGRYPEVDAKRILVQILSATAFFHLQGVVHRDLKPENFLFTSRNEDAILKVIDFG 300

Query: 301 LSDFVKPDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGGRPFWARTESG 360
           LSDF++ D+RLNDVVGSAYYVAPEVL+RSYS EADMWSIGVI+YILLCG RPF+ RTES 
Sbjct: 301 LSDFIRYDQRLNDVVGSAYYVAPEVLHRSYSTEADMWSIGVISYILLCGSRPFYGRTESA 360

Query: 361 IFRSVLRADPNFDDSPWPTISAEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDESLAVPL 420
           IFR VLRA+PNF+D PWP+IS  AKDFVKRLLNKDHRKRMTAAQAL HPWLRDE+  + L
Sbjct: 361 IFRCVLRANPNFEDMPWPSISPTAKDFVKRLLNKDHRKRMTAAQALAHPWLRDENPGLLL 420

Query: 421 DNFIYKSVKAYLRATPFKRAALKALAKALTEDELFYLRTQFKLLEPQNGFVALDNFKAAL 480
           D  +YK VK+Y+RA+PF+R+ALKAL+KA+ ++EL +L+ QF LL+P++G ++L+ F  AL
Sbjct: 421 DFSVYKLVKSYIRASPFRRSALKALSKAIPDEELVFLKAQFMLLDPKDGGLSLNCFTMAL 480

Query: 481 VRNATDAMRESNVADILKMMESLAYKRMDFEEFCAAAISVYQLEAVAGWESIATRAFEYF 540
            R ATDAM ES + DIL  M+ LA K++DFEEFCAAA+SVYQLEA+  WE IAT AFE+F
Sbjct: 481 TRYATDAMMESRLPDILNTMQPLAQKKLDFEEFCAAAVSVYQLEALEEWEQIATSAFEHF 540

Query: 541 EQEGNRVISVHELVQEMNLGPAAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRNSNTR 585
           E EGNR+ISV EL  EM++GP+A+  L++W RSSDGKLSF GY +FLHGVT+R+S++R
Sbjct: 541 EHEGNRIISVQELAGEMSVGPSAYPLLKDWIRSSDGKLSFLGYAKFLHGVTVRSSSSR 592

BLAST of Tan0006333 vs. ExPASy Swiss-Prot
Match: Q9ZUZ2 (CDPK-related kinase 3 OS=Arabidopsis thaliana OX=3702 GN=CRK3 PE=1 SV=1)

HSP 1 Score: 740.7 bits (1911), Expect = 1.2e-212
Identity = 374/564 (66.31%), Postives = 461/564 (81.74%), Query Frame = 0

Query: 32  SPLPSGDAYSRPEESRQSYSVSTTASPF----SSPLPAGIAPSPAR--TPGRKFRWPLPP 91
           +P+  G A + P    +S + S   SPF    +SPLP+G++PSPAR  TP R FR P PP
Sbjct: 37  TPVNYGRAKNTP---ARSSNPSPWPSPFPHGSASPLPSGVSPSPARTSTPRRFFRRPFPP 96

Query: 92  PSPAKPIMAILRR----KSRKASIPEDNQFGEEEVGEIQLDKSFGYSKNFGAKFELGKEI 151
           PSPAK I A L +    K ++  IPE+   G E   E  LDKSFGY KNFGAK+ELGKE+
Sbjct: 97  PSPAKHIKASLIKRLGVKPKEGPIPEER--GTEP--EQSLDKSFGYGKNFGAKYELGKEV 156

Query: 152 GRGHFGHTFWAKGKRGDLKGKPVAVKIISKSKMTSAVGIEDVRREVKILKALSGHKNLVQ 211
           GRGHFGHT   +GK+GD+K  P+AVKIISK+KMT+A+ IEDVRREVK+LK+LSGHK L++
Sbjct: 157 GRGHFGHTCSGRGKKGDIKDHPIAVKIISKAKMTTAIAIEDVRREVKLLKSLSGHKYLIK 216

Query: 212 FHDAFEDTNNIYMVMELCEGGELLERIVSRGGRYPEQESKTIVVQILSVAAFCHLQGVVH 271
           ++DA ED NN+Y+VMELC+GGELL+RI++RGG+YPE ++K IVVQIL+V +FCHLQGVVH
Sbjct: 217 YYDACEDANNVYIVMELCDGGELLDRILARGGKYPEDDAKAIVVQILTVVSFCHLQGVVH 276

Query: 272 RDLKPENFLFVNKDENTGLKVIDFGLSDFVKPDERLNDVVGSAYYVAPEVLYRSYSFEAD 331
           RDLKPENFLF +  E++ LK+IDFGLSDF++PDERLND+VGSAYYVAPEVL+RSYS EAD
Sbjct: 277 RDLKPENFLFTSSREDSDLKLIDFGLSDFIRPDERLNDIVGSAYYVAPEVLHRSYSLEAD 336

Query: 332 MWSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDDSPWPTISAEAKDFVKRLLNKD 391
           +WSIGVI YILLCG RPFWARTESGIFR+VLR +PN+DD PWP+ S+E KDFVKRLLNKD
Sbjct: 337 IWSIGVITYILLCGSRPFWARTESGIFRTVLRTEPNYDDVPWPSCSSEGKDFVKRLLNKD 396

Query: 392 HRKRMTAAQALTHPWLRDESLAVPLDNFIYKSVKAYLRATPFKRAALKALAKALTEDELF 451
           +RKRM+A QALTHPWLRD+S  +PLD  IYK VKAYL ATP +RAALKALAKALTE+EL 
Sbjct: 397 YRKRMSAVQALTHPWLRDDSRVIPLDILIYKLVKAYLHATPLRRAALKALAKALTENELV 456

Query: 452 YLRTQFKLLEP-QNGFVALDNFKAALVRNATDAMRESNVADILKMMESLAYKRMDFEEFC 511
           YLR QF LL P ++G V+L+NFK AL++NATDAMRES V +IL  MESLAY++M FEEFC
Sbjct: 457 YLRAQFMLLGPNKDGSVSLENFKTALMQNATDAMRESRVPEILHTMESLAYRKMYFEEFC 516

Query: 512 AAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPAAHSFLQNWTRSS 571
           AAAIS++QLEAV  WE IAT  F++FE EGNRVI++ EL +E+N+G +A+  L++W RSS
Sbjct: 517 AAAISIHQLEAVDAWEEIATAGFQHFETEGNRVITIEELARELNVGASAYGHLRDWVRSS 576

Query: 572 DGKLSFFGYTRFLHGVTIRNSNTR 585
           DGKLS+ G+T+FLHGVT+R ++ R
Sbjct: 577 DGKLSYLGFTKFLHGVTLRAAHAR 593

BLAST of Tan0006333 vs. ExPASy Swiss-Prot
Match: Q9SCS2 (CDPK-related kinase 5 OS=Arabidopsis thaliana OX=3702 GN=CRK5 PE=2 SV=1)

HSP 1 Score: 669.8 bits (1727), Expect = 2.7e-191
Identity = 336/543 (61.88%), Postives = 430/543 (79.19%), Query Frame = 0

Query: 58  PFSSPLPAGI-------APSPA-----RTPGRKFRWPLPPPSPAKPIMAILRR-----KS 117
           PF SP PA         A SPA      TP R F+ P PPPSPAK I A+L R     K 
Sbjct: 57  PFYSPSPAHYFFSKKTPARSPATNSTNSTPKRFFKRPFPPPSPAKHIRAVLARRHGSVKP 116

Query: 118 RKASIPEDNQFGEEEVGEIQLDKSFGYSKNFGAKFELGKEIGRGHFGHTFWAKGKRGDLK 177
             ++IPE +   E E G + LDKSFG+SK+F +K+ELG E+GRGHFG+T  AK K+GD K
Sbjct: 117 NSSAIPEGS---EAEGGGVGLDKSFGFSKSFASKYELGDEVGRGHFGYTCAAKFKKGDNK 176

Query: 178 GKPVAVKIISKSKMTSAVGIEDVRREVKILKALSGHKNLVQFHDAFEDTNNIYMVMELCE 237
           G+ VAVK+I K+KMT+A+ IEDVRREVKIL+ALSGH NL  F+DA+ED +N+Y+VMELCE
Sbjct: 177 GQQVAVKVIPKAKMTTAIAIEDVRREVKILRALSGHNNLPHFYDAYEDHDNVYIVMELCE 236

Query: 238 GGELLERIVSRGGRYPEQESKTIVVQILSVAAFCHLQGVVHRDLKPENFLFVNKDENTGL 297
           GGELL+RI+SRGG+Y E+++KT+++QIL+V AFCHLQGVVHRDLKPENFLF +K++ + L
Sbjct: 237 GGELLDRILSRGGKYTEEDAKTVMIQILNVVAFCHLQGVVHRDLKPENFLFTSKEDTSQL 296

Query: 298 KVIDFGLSDFVKPDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGGRPFW 357
           K IDFGLSD+V+PDERLND+VGSAYYVAPEVL+RSYS EAD+WS+GVI YILLCG RPFW
Sbjct: 297 KAIDFGLSDYVRPDERLNDIVGSAYYVAPEVLHRSYSTEADIWSVGVIVYILLCGSRPFW 356

Query: 358 ARTESGIFRSVLRADPNFDDSPWPTISAEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDE 417
           ARTESGIFR+VL+ADP+FDD PWP +S+EA+DFVKRLLNKD RKR+TAAQAL+HPW++D 
Sbjct: 357 ARTESGIFRAVLKADPSFDDPPWPLLSSEARDFVKRLLNKDPRKRLTAAQALSHPWIKDS 416

Query: 418 SLA-VPLDNFIYKSVKAYLRATPFKRAALKALAKALTEDELFYLRTQFKLLEP-QNGFVA 477
           + A VP+D  ++K ++AYLR++  ++AAL+AL+K LT DELFYLR QF LLEP +NG ++
Sbjct: 417 NDAKVPMDILVFKLMRAYLRSSSLRKAALRALSKTLTVDELFYLREQFALLEPSKNGTIS 476

Query: 478 LDNFKAALVRNATDAMRESNVADILKMMESLAYKRMDFEEFCAAAISVYQLEAVAGWESI 537
           L+N K+AL++ ATDAM++S + + L  + +L Y+RMDFEEFCAAA+SV+QLEA+  WE  
Sbjct: 477 LENIKSALMKMATDAMKDSRIPEFLGQLSALQYRRMDFEEFCAAALSVHQLEALDRWEQH 536

Query: 538 ATRAFEYFEQEGNRVISVHELVQEMNLGPA--AHSFLQNWTRSSDGKLSFFGYTRFLHGV 580
           A  A+E FE+EGNR I + EL  E+ LGP+   H+ L +W R +DGKLSF G+ + LHGV
Sbjct: 537 ARCAYELFEKEGNRPIMIDELASELGLGPSVPVHAVLHDWLRHTDGKLSFLGFVKLLHGV 596

BLAST of Tan0006333 vs. ExPASy Swiss-Prot
Match: P53681 (CDPK-related protein kinase OS=Daucus carota OX=4039 GN=CRK PE=2 SV=1)

HSP 1 Score: 664.1 bits (1712), Expect = 1.5e-189
Identity = 337/542 (62.18%), Postives = 427/542 (78.78%), Query Frame = 0

Query: 50  YSVSTTASPFSSPLPAGIAP---SPARTPGRKFRWPLPPPSPAKPIMAILRR-----KSR 109
           YS S     FS   PA  +P   S   TP R F  P PPPSPAK I A   R     K  
Sbjct: 61  YSPSPAHFLFSKKSPAVGSPAAGSSNSTPKRLF--PFPPPSPAKHIKAAWARRHGSVKPN 120

Query: 110 KASIPEDNQFGEEEVGEIQLDKSFGYSKNFGAKFELGKEIGRGHFGHTFWAKGKRGDLKG 169
           +A+IPE+N    E  G   LDKSFG+SK FG+KFE+G+E+GRGHFG+T  AK K+G+ KG
Sbjct: 121 EAAIPENN----EVDGGAGLDKSFGFSKKFGSKFEVGEEVGRGHFGYTCRAKFKKGEFKG 180

Query: 170 KPVAVKIISKSKMTSAVGIEDVRREVKILKALSGHKNLVQFHDAFEDTNNIYMVMELCEG 229
           + VAVK+I K+KMT+A+ IEDVRREVKIL+AL+GH NLVQF+DAFED  N+Y+VMELCEG
Sbjct: 181 QDVAVKVIPKAKMTTAIAIEDVRREVKILRALTGHNNLVQFYDAFEDHTNVYVVMELCEG 240

Query: 230 GELLERIVSRGGRYPEQESKTIVVQILSVAAFCHLQGVVHRDLKPENFLFVNKDENTGLK 289
           GELL+RI+SRGG+Y E ++K +++QIL+V AFCHLQGVVHRDLKPENFLF +KDE++ LK
Sbjct: 241 GELLDRILSRGGKYTEDDAKAVMIQILNVVAFCHLQGVVHRDLKPENFLFKSKDEDSQLK 300

Query: 290 VIDFGLSDFVKPDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGGRPFWA 349
            IDFGLSD+VKPDERLND+VGSAYYVAPEVL+RSYS EAD+WSIGVI+YILLCG RPFWA
Sbjct: 301 AIDFGLSDYVKPDERLNDIVGSAYYVAPEVLHRSYSTEADVWSIGVISYILLCGSRPFWA 360

Query: 350 RTESGIFRSVLRADPNFDDSPWPTISAEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDES 409
           RTESGIFR+VL+A+ +FD+ PWP++S+EAKDFVKRLLNKD RKRMTAAQAL H W+++ +
Sbjct: 361 RTESGIFRAVLKANLSFDEPPWPSVSSEAKDFVKRLLNKDPRKRMTAAQALCHSWIKNSN 420

Query: 410 -LAVPLDNFIYKSVKAYLRATPFKRAALKALAKALTEDELFYLRTQFKLLEP-QNGFVAL 469
            +  PLD  ++K +K Y+R++P ++AAL+AL+K LT DELFYL+ QF LLEP +NG ++L
Sbjct: 421 DIKFPLDILVFKLMKVYMRSSPLRKAALRALSKTLTVDELFYLKEQFVLLEPTKNGTISL 480

Query: 470 DNFKAALVRNATDAMRESNVADILKMMESLAYKRMDFEEFCAAAISVYQLEAVAGWESIA 529
           +N K AL+RN+TDAM++S V D+L  + +L Y+RMDFEEFCAAA+SV+QLEA+  WE  A
Sbjct: 481 ENIKQALMRNSTDAMKDSRVLDLLVSLNALQYRRMDFEEFCAAALSVHQLEALDRWEQHA 540

Query: 530 TRAFEYFEQEGNRVISVHELVQEMNLGPA--AHSFLQNWTRSSDGKLSFFGYTRFLHGVT 580
             A++ FE++GNR I + EL  E+ LGP+   H+ L +W R +DGKLSF GY + LHGV+
Sbjct: 541 RCAYDLFEKDGNRAIMIEELASELGLGPSIPVHAVLHDWIRHTDGKLSFLGYVKLLHGVS 596

BLAST of Tan0006333 vs. NCBI nr
Match: XP_022958285.1 (CDPK-related kinase 6-like [Cucurbita moschata])

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 539/585 (92.14%), Postives = 559/585 (95.56%), Query Frame = 0

Query: 1   MGHFCSKSVSDVNNDTAISGGTRPRSSHSGASPLPSGDAYSRPEESRQSYSVSTTASPFS 60
           MGH CSKSVSDVN+DT ISGG+ PRSSHSGASP+  GD YSRPEE RQSYSVS T SPFS
Sbjct: 1   MGHLCSKSVSDVNHDTGISGGSHPRSSHSGASPIAYGDVYSRPEEFRQSYSVSVTGSPFS 60

Query: 61  SPLPAGIAPSPARTPGRKFRWPLPPPSPAKPIMAILRRKSRKASIPEDNQFGEEEVGEIQ 120
           SPLPAGIAPSPARTPGRKFRWPLPPPSPA+PIMAILRRKSRKASIPE+N+ GEE VGEIQ
Sbjct: 61  SPLPAGIAPSPARTPGRKFRWPLPPPSPARPIMAILRRKSRKASIPEENRIGEEGVGEIQ 120

Query: 121 LDKSFGYSKNFGAKFELGKEIGRGHFGHTFWAKGKRGDLKGKPVAVKIISKSKMTSAVGI 180
           LDKSFGYSKNFGAKFELGKEIGRGHFGHTFWA+GK+GDLKGKPVAVKIISKSKMTSAVGI
Sbjct: 121 LDKSFGYSKNFGAKFELGKEIGRGHFGHTFWARGKKGDLKGKPVAVKIISKSKMTSAVGI 180

Query: 181 EDVRREVKILKALSGHKNLVQFHDAFEDTNNIYMVMELCEGGELLERIVSRGGRYPEQES 240
           EDVRREVKILKALSGHKNLVQFHDAFED NNIYMVMELCEGGELLERIVSRGGRYPEQES
Sbjct: 181 EDVRREVKILKALSGHKNLVQFHDAFEDANNIYMVMELCEGGELLERIVSRGGRYPEQES 240

Query: 241 KTIVVQILSVAAFCHLQGVVHRDLKPENFLFVNKDENTGLKVIDFGLSDFVKPDERLNDV 300
           KTI+VQIL+V AF HLQGVVHRDLKPENFLFV KDENTGLKVIDFGLSDF+KPDE LNDV
Sbjct: 241 KTILVQILNVVAFFHLQGVVHRDLKPENFLFVKKDENTGLKVIDFGLSDFIKPDEHLNDV 300

Query: 301 VGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDD 360
           VGSAYYVAPEVLYRSYS EAD+WSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDD
Sbjct: 301 VGSAYYVAPEVLYRSYSHEADVWSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDD 360

Query: 361 SPWPTISAEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDESLAVPLDNFIYKSVKAYLRA 420
           SPWPTISAEAKDFVKRLLNKDHRKRMTA QALTHPWLRDE++AVPLD+FIYKSVKAYLR 
Sbjct: 361 SPWPTISAEAKDFVKRLLNKDHRKRMTAVQALTHPWLRDENIAVPLDSFIYKSVKAYLRV 420

Query: 421 TPFKRAALKALAKALTEDELFYLRTQFKLLEPQNGFVALDNFKAALVRNATDAMRESNVA 480
           TPFKRAALKALAKALTEDELFYLR QFKLLEPQNGFV LDNFK ALVRNATDAMRESNV 
Sbjct: 421 TPFKRAALKALAKALTEDELFYLRAQFKLLEPQNGFVTLDNFKEALVRNATDAMRESNVL 480

Query: 481 DILKMMESLAYKRMDFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELV 540
           DILKM ES+AY+RMDFEEFCAAAISVYQLEAVAGWE IAT+AFEYFEQ+GNRVISVHELV
Sbjct: 481 DILKMTESVAYERMDFEEFCAAAISVYQLEAVAGWEGIATQAFEYFEQDGNRVISVHELV 540

Query: 541 QEMNLGPAAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRNSNTRH 586
           QEMNL PAAHSFLQNWTRSSDGKLSFFGYTRFLHGVT+RNSNTRH
Sbjct: 541 QEMNLSPAAHSFLQNWTRSSDGKLSFFGYTRFLHGVTVRNSNTRH 585

BLAST of Tan0006333 vs. NCBI nr
Match: XP_022995579.1 (CDPK-related kinase 6-like [Cucurbita maxima])

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 541/585 (92.48%), Postives = 558/585 (95.38%), Query Frame = 0

Query: 1   MGHFCSKSVSDVNNDTAISGGTRPRSSHSGASPLPSGDAYSRPEESRQSYSVSTTASPFS 60
           MGH CSKSVSDVN+DT ISGG+  RSSHSGASP+  GD YSRPEE RQSYSVS T SPFS
Sbjct: 1   MGHLCSKSVSDVNHDTGISGGSHTRSSHSGASPIAYGDVYSRPEEFRQSYSVSATGSPFS 60

Query: 61  SPLPAGIAPSPARTPGRKFRWPLPPPSPAKPIMAILRRKSRKASIPEDNQFGEEEVGEIQ 120
           SPLPAGIAPSPARTPGRKFRWPLPPPSPAKPIMAILRRKSRKASIPE+N+ GEE VGEIQ
Sbjct: 61  SPLPAGIAPSPARTPGRKFRWPLPPPSPAKPIMAILRRKSRKASIPEENRIGEEGVGEIQ 120

Query: 121 LDKSFGYSKNFGAKFELGKEIGRGHFGHTFWAKGKRGDLKGKPVAVKIISKSKMTSAVGI 180
           LDKSFGYSKNFGAKFELGKEIGRGHFGHTFWA+GK+GDLKGKPVAVKIISKSKMTSAVGI
Sbjct: 121 LDKSFGYSKNFGAKFELGKEIGRGHFGHTFWARGKKGDLKGKPVAVKIISKSKMTSAVGI 180

Query: 181 EDVRREVKILKALSGHKNLVQFHDAFEDTNNIYMVMELCEGGELLERIVSRGGRYPEQES 240
           EDVRREVKILKALSGHKNLVQFHDAFED NNIYMVMELCEGGELLERIVSRGGRYPEQES
Sbjct: 181 EDVRREVKILKALSGHKNLVQFHDAFEDANNIYMVMELCEGGELLERIVSRGGRYPEQES 240

Query: 241 KTIVVQILSVAAFCHLQGVVHRDLKPENFLFVNKDENTGLKVIDFGLSDFVKPDERLNDV 300
           KTI+VQIL+V AF HLQGVVHRDLKPENFLFV KDENTGLKVIDFGLSDFVKPDE LNDV
Sbjct: 241 KTILVQILNVVAFFHLQGVVHRDLKPENFLFVKKDENTGLKVIDFGLSDFVKPDEHLNDV 300

Query: 301 VGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDD 360
           VGSAYYVAPEVLYRSYS EAD+WSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDD
Sbjct: 301 VGSAYYVAPEVLYRSYSHEADVWSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDD 360

Query: 361 SPWPTISAEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDESLAVPLDNFIYKSVKAYLRA 420
           SPWPTISAEAKDFVKRLLNKDHRKRMTA QALTHPWLRDE++AVPLD+FIYKSVKAYLR 
Sbjct: 361 SPWPTISAEAKDFVKRLLNKDHRKRMTAVQALTHPWLRDENVAVPLDSFIYKSVKAYLRV 420

Query: 421 TPFKRAALKALAKALTEDELFYLRTQFKLLEPQNGFVALDNFKAALVRNATDAMRESNVA 480
           TPFKRAALKALAKALTEDELFYLR QFKLLEPQNGFV LDNFK ALVRNATDAMRESNV 
Sbjct: 421 TPFKRAALKALAKALTEDELFYLRAQFKLLEPQNGFVTLDNFKEALVRNATDAMRESNVL 480

Query: 481 DILKMMESLAYKRMDFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELV 540
           DILKM ES+AY+RMDFEEFCAAAISVYQLEAVAGWE IATRAFEYFEQ+GNRVISVHELV
Sbjct: 481 DILKMTESIAYERMDFEEFCAAAISVYQLEAVAGWEGIATRAFEYFEQDGNRVISVHELV 540

Query: 541 QEMNLGPAAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRNSNTRH 586
           QEMNL PAAHSFLQNWTRSSDGKLSFFGYTRFLHGVT+RNSNTRH
Sbjct: 541 QEMNLSPAAHSFLQNWTRSSDGKLSFFGYTRFLHGVTVRNSNTRH 585

BLAST of Tan0006333 vs. NCBI nr
Match: XP_023534615.1 (CDPK-related kinase 6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1083.6 bits (2801), Expect = 0.0e+00
Identity = 538/585 (91.97%), Postives = 558/585 (95.38%), Query Frame = 0

Query: 1   MGHFCSKSVSDVNNDTAISGGTRPRSSHSGASPLPSGDAYSRPEESRQSYSVSTTASPFS 60
           MGH CSKSVSDVN+DT ISGG+  RSSHSGASP+  GD YSRPEE RQSYSVS T SPFS
Sbjct: 1   MGHLCSKSVSDVNHDTGISGGSHTRSSHSGASPIAYGDVYSRPEEFRQSYSVSATGSPFS 60

Query: 61  SPLPAGIAPSPARTPGRKFRWPLPPPSPAKPIMAILRRKSRKASIPEDNQFGEEEVGEIQ 120
           SPLPAGIAPSPARTPGRKFRWPLPPPSPAKPIMAILRRKSRKA+IPE+N+ GEE VGEIQ
Sbjct: 61  SPLPAGIAPSPARTPGRKFRWPLPPPSPAKPIMAILRRKSRKATIPEENRIGEEGVGEIQ 120

Query: 121 LDKSFGYSKNFGAKFELGKEIGRGHFGHTFWAKGKRGDLKGKPVAVKIISKSKMTSAVGI 180
           LDKSFGYSKNFGAKFELGKEIGRGHFGHTFWA+GK+GDLKGKPVAVKIISKSKMTSAVGI
Sbjct: 121 LDKSFGYSKNFGAKFELGKEIGRGHFGHTFWARGKKGDLKGKPVAVKIISKSKMTSAVGI 180

Query: 181 EDVRREVKILKALSGHKNLVQFHDAFEDTNNIYMVMELCEGGELLERIVSRGGRYPEQES 240
           EDVRREVKILKALSGHKNLVQFHDAFED NNIYMVMELCEGGELLERIVSRGGRYPEQES
Sbjct: 181 EDVRREVKILKALSGHKNLVQFHDAFEDANNIYMVMELCEGGELLERIVSRGGRYPEQES 240

Query: 241 KTIVVQILSVAAFCHLQGVVHRDLKPENFLFVNKDENTGLKVIDFGLSDFVKPDERLNDV 300
           KTI+VQIL+V AF HLQGVVHRDLKPENFLFV KDENTGLKVIDFGLSDFVKPDE LNDV
Sbjct: 241 KTILVQILNVVAFFHLQGVVHRDLKPENFLFVKKDENTGLKVIDFGLSDFVKPDEHLNDV 300

Query: 301 VGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDD 360
           VGSAYYVAPEVLYRSYS EAD+WSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDD
Sbjct: 301 VGSAYYVAPEVLYRSYSHEADVWSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDD 360

Query: 361 SPWPTISAEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDESLAVPLDNFIYKSVKAYLRA 420
           SPWPTISAEAKDFVKRLLNKDHRKRMTA QALTHPWLRDE++AVPLD+FIYKSVKAYLR 
Sbjct: 361 SPWPTISAEAKDFVKRLLNKDHRKRMTAVQALTHPWLRDENVAVPLDSFIYKSVKAYLRV 420

Query: 421 TPFKRAALKALAKALTEDELFYLRTQFKLLEPQNGFVALDNFKAALVRNATDAMRESNVA 480
           TPFKRAALKALAKALTEDELFYLR QFKLLEPQNGFV +DNFK ALVRNATDAMRESN+ 
Sbjct: 421 TPFKRAALKALAKALTEDELFYLRAQFKLLEPQNGFVTIDNFKEALVRNATDAMRESNIL 480

Query: 481 DILKMMESLAYKRMDFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELV 540
           DILKM ES+AY+RMDFEEFCAAAISVYQLEAVAGWE IATRAFEYFEQ+GNRVISVHELV
Sbjct: 481 DILKMTESVAYERMDFEEFCAAAISVYQLEAVAGWEGIATRAFEYFEQDGNRVISVHELV 540

Query: 541 QEMNLGPAAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRNSNTRH 586
           QEMNL PAAHSFLQNWTRSSDGKLSFFGYTRFLHGVT+RNSNTRH
Sbjct: 541 QEMNLSPAAHSFLQNWTRSSDGKLSFFGYTRFLHGVTVRNSNTRH 585

BLAST of Tan0006333 vs. NCBI nr
Match: KAG6605809.1 (CDPK-related kinase 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1081.2 bits (2795), Expect = 0.0e+00
Identity = 537/585 (91.79%), Postives = 557/585 (95.21%), Query Frame = 0

Query: 1   MGHFCSKSVSDVNNDTAISGGTRPRSSHSGASPLPSGDAYSRPEESRQSYSVSTTASPFS 60
           MGH CSKSVSDVN+DT ISGG+  RSSHSGASP+  GD YSRPEE RQSYSVS T SPFS
Sbjct: 1   MGHLCSKSVSDVNHDTGISGGSHTRSSHSGASPIAYGDVYSRPEEFRQSYSVSVTGSPFS 60

Query: 61  SPLPAGIAPSPARTPGRKFRWPLPPPSPAKPIMAILRRKSRKASIPEDNQFGEEEVGEIQ 120
           SPLPAGIAPSPARTPGRKFRWPLPPPSPAKPIMAILRRKSRKASIPE+N+ GEE VGEIQ
Sbjct: 61  SPLPAGIAPSPARTPGRKFRWPLPPPSPAKPIMAILRRKSRKASIPEENRIGEEGVGEIQ 120

Query: 121 LDKSFGYSKNFGAKFELGKEIGRGHFGHTFWAKGKRGDLKGKPVAVKIISKSKMTSAVGI 180
           LDKSFGYSKNFGAKFELGKEIGRGHFGHTFWA+GK+GDLKGKPVAVKIISKSKMTSAVGI
Sbjct: 121 LDKSFGYSKNFGAKFELGKEIGRGHFGHTFWARGKKGDLKGKPVAVKIISKSKMTSAVGI 180

Query: 181 EDVRREVKILKALSGHKNLVQFHDAFEDTNNIYMVMELCEGGELLERIVSRGGRYPEQES 240
           EDVRREVKILKALSGHKNLVQFHDAFED NNIYMVMELCEGGELLERIVSRGGRYPEQES
Sbjct: 181 EDVRREVKILKALSGHKNLVQFHDAFEDANNIYMVMELCEGGELLERIVSRGGRYPEQES 240

Query: 241 KTIVVQILSVAAFCHLQGVVHRDLKPENFLFVNKDENTGLKVIDFGLSDFVKPDERLNDV 300
           KTI+VQIL+V AF H+QGVVHRDLKPENFLFV KDENTGLKVIDFGLSDF+KPDE LNDV
Sbjct: 241 KTILVQILNVVAFFHIQGVVHRDLKPENFLFVKKDENTGLKVIDFGLSDFIKPDEHLNDV 300

Query: 301 VGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDD 360
           VGSAYYVAPEVLYRSYS EAD+WSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDD
Sbjct: 301 VGSAYYVAPEVLYRSYSHEADVWSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDD 360

Query: 361 SPWPTISAEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDESLAVPLDNFIYKSVKAYLRA 420
           SPWPTISAEAKDFVKRLLNKDHRKRMTA QALTHPWLRDE++AVPLD+FIYKSVKAYLR 
Sbjct: 361 SPWPTISAEAKDFVKRLLNKDHRKRMTAVQALTHPWLRDENIAVPLDSFIYKSVKAYLRV 420

Query: 421 TPFKRAALKALAKALTEDELFYLRTQFKLLEPQNGFVALDNFKAALVRNATDAMRESNVA 480
           TPFKRAALKALAKALTEDELFYLR QFKLLEPQNGFV +DNFK ALVRNATDAMRESNV 
Sbjct: 421 TPFKRAALKALAKALTEDELFYLRAQFKLLEPQNGFVTIDNFKEALVRNATDAMRESNVL 480

Query: 481 DILKMMESLAYKRMDFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELV 540
           DILKM ES+AY+RMDFEEFCAAAISVYQLEAVAGWE IATRAFEYFEQ+GNRVISVHELV
Sbjct: 481 DILKMTESVAYERMDFEEFCAAAISVYQLEAVAGWEGIATRAFEYFEQDGNRVISVHELV 540

Query: 541 QEMNLGPAAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRNSNTRH 586
           QEMNL  AAHSFLQNWTRSSDGKLSFFGYTRFLHGVT+RNSNTRH
Sbjct: 541 QEMNLSTAAHSFLQNWTRSSDGKLSFFGYTRFLHGVTVRNSNTRH 585

BLAST of Tan0006333 vs. NCBI nr
Match: KAG7035771.1 (CDPK-related kinase 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1043.5 bits (2697), Expect = 6.9e-301
Identity = 533/633 (84.20%), Postives = 553/633 (87.36%), Query Frame = 0

Query: 1   MGHFCSKSVSDVNNDTAISGGTRPRSSHSGASPLPSGDAYSRPEESRQSYSVSTTASPFS 60
           MGH CSKSVSDVN+DT ISGG+  RSSHSGASP+  GD YSRPEE RQSYSVS T SPFS
Sbjct: 1   MGHLCSKSVSDVNHDTGISGGSHTRSSHSGASPIAYGDVYSRPEEFRQSYSVSVTGSPFS 60

Query: 61  SPLPAGIAPSPARTPGRKFRWPLPPPSPAKPIMAILRRKSRKA--SIPEDNQFGEEEVGE 120
           SPLPAGIAPSPARTPGRKFRWPLPPPSPAKPIMAILRRKSRKA  SIPE+N+ GEE VGE
Sbjct: 61  SPLPAGIAPSPARTPGRKFRWPLPPPSPAKPIMAILRRKSRKASTSIPEENRIGEEGVGE 120

Query: 121 IQLDKSFGYSKNFGAKFELGKEIGRGHFGHTFWAKGKRGDLKGKPVAVKIISKSKMTSAV 180
           IQLDKSFGYSKNFGAKFELGKEIGRGHFGHTFWA+GK+GDLKGKPVAVKIISKSKMTSAV
Sbjct: 121 IQLDKSFGYSKNFGAKFELGKEIGRGHFGHTFWARGKKGDLKGKPVAVKIISKSKMTSAV 180

Query: 181 GIEDVRREVKILKALSGHKNLVQFHDAFEDTNNIYMVMELCEGGELLERIVSRGGRYPEQ 240
           GIEDVRREVKILKALSGHKNLVQFHDAFED NNIYMVMELCEGGELLERIVSRGGRYPEQ
Sbjct: 181 GIEDVRREVKILKALSGHKNLVQFHDAFEDANNIYMVMELCEGGELLERIVSRGGRYPEQ 240

Query: 241 ESKTIVVQILSVAAFCHLQGVVHRDLKPENFLFVNKDENTGLKVIDFGLSDFVKP----- 300
           ESKTI+VQIL+V AF HLQGVVHRDLKPENFLFV KDENTGLKVIDFGLSDF+KP     
Sbjct: 241 ESKTILVQILNVVAFFHLQGVVHRDLKPENFLFVKKDENTGLKVIDFGLSDFIKPVGIKQ 300

Query: 301 ---------------------------------------------DERLNDVVGSAYYVA 360
                                                        DE LNDVVGSAYYVA
Sbjct: 301 TLGGVIISSHISWRGNETFFIRLVSELETGGVPVRTPSPEWGCCADEHLNDVVGSAYYVA 360

Query: 361 PEVLYRSYSFEADMWSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDDSPWPTISA 420
           PEVLYRSYS EAD+WSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDDSPWPTISA
Sbjct: 361 PEVLYRSYSHEADVWSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDDSPWPTISA 420

Query: 421 EAKDFVKRLLNKDHRKRMTAAQALTHPWLRDESLAVPLDNFIYKSVKAYLRATPFKRAAL 480
           EAKDFVKRLLNKDHRKRMTA QALTHPWLRDE++AVPLD+FIYKSVKAYLR TPFKRAAL
Sbjct: 421 EAKDFVKRLLNKDHRKRMTAVQALTHPWLRDENIAVPLDSFIYKSVKAYLRVTPFKRAAL 480

Query: 481 KALAKALTEDELFYLRTQFKLLEPQNGFVALDNFKAALVRNATDAMRESNVADILKMMES 540
           KALAKALTEDELFYLR QFKLLEPQNGFV +DNFK ALVRNATDAMRESNV DILKM ES
Sbjct: 481 KALAKALTEDELFYLRAQFKLLEPQNGFVTIDNFKEALVRNATDAMRESNVLDILKMTES 540

Query: 541 LAYKRMDFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPA 582
           +AY+RMDFEEFCAAAISVYQLEAVAGWE IATRAFEYFEQ+GNRVISVHELVQEMNL PA
Sbjct: 541 VAYERMDFEEFCAAAISVYQLEAVAGWEGIATRAFEYFEQDGNRVISVHELVQEMNLSPA 600

BLAST of Tan0006333 vs. ExPASy TrEMBL
Match: A0A6J1K6B2 (CDPK-related kinase 6-like OS=Cucurbita maxima OX=3661 GN=LOC111491067 PE=4 SV=1)

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 541/585 (92.48%), Postives = 558/585 (95.38%), Query Frame = 0

Query: 1   MGHFCSKSVSDVNNDTAISGGTRPRSSHSGASPLPSGDAYSRPEESRQSYSVSTTASPFS 60
           MGH CSKSVSDVN+DT ISGG+  RSSHSGASP+  GD YSRPEE RQSYSVS T SPFS
Sbjct: 1   MGHLCSKSVSDVNHDTGISGGSHTRSSHSGASPIAYGDVYSRPEEFRQSYSVSATGSPFS 60

Query: 61  SPLPAGIAPSPARTPGRKFRWPLPPPSPAKPIMAILRRKSRKASIPEDNQFGEEEVGEIQ 120
           SPLPAGIAPSPARTPGRKFRWPLPPPSPAKPIMAILRRKSRKASIPE+N+ GEE VGEIQ
Sbjct: 61  SPLPAGIAPSPARTPGRKFRWPLPPPSPAKPIMAILRRKSRKASIPEENRIGEEGVGEIQ 120

Query: 121 LDKSFGYSKNFGAKFELGKEIGRGHFGHTFWAKGKRGDLKGKPVAVKIISKSKMTSAVGI 180
           LDKSFGYSKNFGAKFELGKEIGRGHFGHTFWA+GK+GDLKGKPVAVKIISKSKMTSAVGI
Sbjct: 121 LDKSFGYSKNFGAKFELGKEIGRGHFGHTFWARGKKGDLKGKPVAVKIISKSKMTSAVGI 180

Query: 181 EDVRREVKILKALSGHKNLVQFHDAFEDTNNIYMVMELCEGGELLERIVSRGGRYPEQES 240
           EDVRREVKILKALSGHKNLVQFHDAFED NNIYMVMELCEGGELLERIVSRGGRYPEQES
Sbjct: 181 EDVRREVKILKALSGHKNLVQFHDAFEDANNIYMVMELCEGGELLERIVSRGGRYPEQES 240

Query: 241 KTIVVQILSVAAFCHLQGVVHRDLKPENFLFVNKDENTGLKVIDFGLSDFVKPDERLNDV 300
           KTI+VQIL+V AF HLQGVVHRDLKPENFLFV KDENTGLKVIDFGLSDFVKPDE LNDV
Sbjct: 241 KTILVQILNVVAFFHLQGVVHRDLKPENFLFVKKDENTGLKVIDFGLSDFVKPDEHLNDV 300

Query: 301 VGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDD 360
           VGSAYYVAPEVLYRSYS EAD+WSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDD
Sbjct: 301 VGSAYYVAPEVLYRSYSHEADVWSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDD 360

Query: 361 SPWPTISAEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDESLAVPLDNFIYKSVKAYLRA 420
           SPWPTISAEAKDFVKRLLNKDHRKRMTA QALTHPWLRDE++AVPLD+FIYKSVKAYLR 
Sbjct: 361 SPWPTISAEAKDFVKRLLNKDHRKRMTAVQALTHPWLRDENVAVPLDSFIYKSVKAYLRV 420

Query: 421 TPFKRAALKALAKALTEDELFYLRTQFKLLEPQNGFVALDNFKAALVRNATDAMRESNVA 480
           TPFKRAALKALAKALTEDELFYLR QFKLLEPQNGFV LDNFK ALVRNATDAMRESNV 
Sbjct: 421 TPFKRAALKALAKALTEDELFYLRAQFKLLEPQNGFVTLDNFKEALVRNATDAMRESNVL 480

Query: 481 DILKMMESLAYKRMDFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELV 540
           DILKM ES+AY+RMDFEEFCAAAISVYQLEAVAGWE IATRAFEYFEQ+GNRVISVHELV
Sbjct: 481 DILKMTESIAYERMDFEEFCAAAISVYQLEAVAGWEGIATRAFEYFEQDGNRVISVHELV 540

Query: 541 QEMNLGPAAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRNSNTRH 586
           QEMNL PAAHSFLQNWTRSSDGKLSFFGYTRFLHGVT+RNSNTRH
Sbjct: 541 QEMNLSPAAHSFLQNWTRSSDGKLSFFGYTRFLHGVTVRNSNTRH 585

BLAST of Tan0006333 vs. ExPASy TrEMBL
Match: A0A6J1H327 (CDPK-related kinase 6-like OS=Cucurbita moschata OX=3662 GN=LOC111459553 PE=4 SV=1)

HSP 1 Score: 1086.2 bits (2808), Expect = 0.0e+00
Identity = 539/585 (92.14%), Postives = 559/585 (95.56%), Query Frame = 0

Query: 1   MGHFCSKSVSDVNNDTAISGGTRPRSSHSGASPLPSGDAYSRPEESRQSYSVSTTASPFS 60
           MGH CSKSVSDVN+DT ISGG+ PRSSHSGASP+  GD YSRPEE RQSYSVS T SPFS
Sbjct: 1   MGHLCSKSVSDVNHDTGISGGSHPRSSHSGASPIAYGDVYSRPEEFRQSYSVSVTGSPFS 60

Query: 61  SPLPAGIAPSPARTPGRKFRWPLPPPSPAKPIMAILRRKSRKASIPEDNQFGEEEVGEIQ 120
           SPLPAGIAPSPARTPGRKFRWPLPPPSPA+PIMAILRRKSRKASIPE+N+ GEE VGEIQ
Sbjct: 61  SPLPAGIAPSPARTPGRKFRWPLPPPSPARPIMAILRRKSRKASIPEENRIGEEGVGEIQ 120

Query: 121 LDKSFGYSKNFGAKFELGKEIGRGHFGHTFWAKGKRGDLKGKPVAVKIISKSKMTSAVGI 180
           LDKSFGYSKNFGAKFELGKEIGRGHFGHTFWA+GK+GDLKGKPVAVKIISKSKMTSAVGI
Sbjct: 121 LDKSFGYSKNFGAKFELGKEIGRGHFGHTFWARGKKGDLKGKPVAVKIISKSKMTSAVGI 180

Query: 181 EDVRREVKILKALSGHKNLVQFHDAFEDTNNIYMVMELCEGGELLERIVSRGGRYPEQES 240
           EDVRREVKILKALSGHKNLVQFHDAFED NNIYMVMELCEGGELLERIVSRGGRYPEQES
Sbjct: 181 EDVRREVKILKALSGHKNLVQFHDAFEDANNIYMVMELCEGGELLERIVSRGGRYPEQES 240

Query: 241 KTIVVQILSVAAFCHLQGVVHRDLKPENFLFVNKDENTGLKVIDFGLSDFVKPDERLNDV 300
           KTI+VQIL+V AF HLQGVVHRDLKPENFLFV KDENTGLKVIDFGLSDF+KPDE LNDV
Sbjct: 241 KTILVQILNVVAFFHLQGVVHRDLKPENFLFVKKDENTGLKVIDFGLSDFIKPDEHLNDV 300

Query: 301 VGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDD 360
           VGSAYYVAPEVLYRSYS EAD+WSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDD
Sbjct: 301 VGSAYYVAPEVLYRSYSHEADVWSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDD 360

Query: 361 SPWPTISAEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDESLAVPLDNFIYKSVKAYLRA 420
           SPWPTISAEAKDFVKRLLNKDHRKRMTA QALTHPWLRDE++AVPLD+FIYKSVKAYLR 
Sbjct: 361 SPWPTISAEAKDFVKRLLNKDHRKRMTAVQALTHPWLRDENIAVPLDSFIYKSVKAYLRV 420

Query: 421 TPFKRAALKALAKALTEDELFYLRTQFKLLEPQNGFVALDNFKAALVRNATDAMRESNVA 480
           TPFKRAALKALAKALTEDELFYLR QFKLLEPQNGFV LDNFK ALVRNATDAMRESNV 
Sbjct: 421 TPFKRAALKALAKALTEDELFYLRAQFKLLEPQNGFVTLDNFKEALVRNATDAMRESNVL 480

Query: 481 DILKMMESLAYKRMDFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELV 540
           DILKM ES+AY+RMDFEEFCAAAISVYQLEAVAGWE IAT+AFEYFEQ+GNRVISVHELV
Sbjct: 481 DILKMTESVAYERMDFEEFCAAAISVYQLEAVAGWEGIATQAFEYFEQDGNRVISVHELV 540

Query: 541 QEMNLGPAAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRNSNTRH 586
           QEMNL PAAHSFLQNWTRSSDGKLSFFGYTRFLHGVT+RNSNTRH
Sbjct: 541 QEMNLSPAAHSFLQNWTRSSDGKLSFFGYTRFLHGVTVRNSNTRH 585

BLAST of Tan0006333 vs. ExPASy TrEMBL
Match: A0A6J1E874 (CDPK-related kinase 4 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430287 PE=4 SV=1)

HSP 1 Score: 1027.3 bits (2655), Expect = 2.5e-296
Identity = 515/585 (88.03%), Postives = 535/585 (91.45%), Query Frame = 0

Query: 1   MGHFCSKSVSDVNNDTAISGGTRPRSSHSGASPLPSGDAYSRPEESRQSYSVSTTASPFS 60
           MGH CSKSVSD++N   ISGG+ P                   E++R S SVS TASPFS
Sbjct: 1   MGHHCSKSVSDISNHAVISGGSHP------------------TEDTRHSNSVSATASPFS 60

Query: 61  SPLPAGIAPSPARTPGRKFRWPLPPPSPAKPIMAILRRKSRKASIPEDNQFGEEEVGEIQ 120
           SPLP+GIAPSPART  RKFRWPLPPPSPAKPIMAILRRKSRKASIPE+NQ GEE +GE+Q
Sbjct: 61  SPLPSGIAPSPARTTRRKFRWPLPPPSPAKPIMAILRRKSRKASIPEENQIGEEGLGEVQ 120

Query: 121 LDKSFGYSKNFGAKFELGKEIGRGHFGHTFWAKGKRGDLKGKPVAVKIISKSKMTSAVGI 180
           LDKSFGYS NFGAKFELG+E+GRGHFGHTFWAKGK+GDLKGKPVAVKIISKSKMTS VGI
Sbjct: 121 LDKSFGYSTNFGAKFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKSKMTSTVGI 180

Query: 181 EDVRREVKILKALSGHKNLVQFHDAFEDTNNIYMVMELCEGGELLERIVSRGGRYPEQES 240
           EDVRREVKILKALSGHKNLV FH AFED NNIY+VMELCEGGELLERIVSRGGRY EQES
Sbjct: 181 EDVRREVKILKALSGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQES 240

Query: 241 KTIVVQILSVAAFCHLQGVVHRDLKPENFLFVNKDENTGLKVIDFGLSDFVKPDERLNDV 300
           KTI+VQIL V AFCHLQG+VHRDLKPENFLF+ KDENTGLK+IDFGLSDFVKPDERLNDV
Sbjct: 241 KTIIVQILCVVAFCHLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDV 300

Query: 301 VGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDD 360
           VGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCG RPFWARTESGIFRSVLRADPNFDD
Sbjct: 301 VGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDD 360

Query: 361 SPWPTISAEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDESLAVPLDNFIYKSVKAYLRA 420
           SPWPTIS EAKDFVKRLLNKDHRKRMTAAQALTHPWLRDE LAVPLDNFIYKSVKAYLRA
Sbjct: 361 SPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRA 420

Query: 421 TPFKRAALKALAKALTEDELFYLRTQFKLLEPQNGFVALDNFKAALVRNATDAMRESNVA 480
           TPFKRAALKALAKALTEDELFYLRTQFKLL+PQNGFV +DN+KAALVRN TDAMRESNVA
Sbjct: 421 TPFKRAALKALAKALTEDELFYLRTQFKLLDPQNGFVTIDNYKAALVRNVTDAMRESNVA 480

Query: 481 DILKMMESLAYKRMDFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELV 540
           DILKMME LA KRM FEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELV
Sbjct: 481 DILKMMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELV 540

Query: 541 QEMNLGPAAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRNSNTRH 586
           QEMNLGP AHSFLQNWTRSSDGKLSFFGYTRFLHGVTIR SNTRH
Sbjct: 541 QEMNLGPIAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRYSNTRH 567

BLAST of Tan0006333 vs. ExPASy TrEMBL
Match: A0A6J1IDP0 (CDPK-related kinase 6-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111472964 PE=4 SV=1)

HSP 1 Score: 1024.2 bits (2647), Expect = 2.1e-295
Identity = 517/585 (88.38%), Postives = 540/585 (92.31%), Query Frame = 0

Query: 1   MGHFCSKSVSDVNNDTAISGGTRPRSSHSGASPLPSGDAYSRPEESRQSYSVSTTASPFS 60
           MGH CSKSVSDV++  AISGG+RP S     SP+        PE++R S SVS TASPFS
Sbjct: 1   MGHHCSKSVSDVSHHAAISGGSRPSS-----SPI--------PEDTRHSNSVSATASPFS 60

Query: 61  SPLPAGIAPSPARTPGRKFRWPLPPPSPAKPIMAILRRKSRKASIPEDNQFGEEEVGEIQ 120
           SPLP+ IAPSPART  RKFRWPLPPPSPAKPIMAILR+KSRKASIPE+NQ GEE +GE+Q
Sbjct: 61  SPLPSRIAPSPART-RRKFRWPLPPPSPAKPIMAILRQKSRKASIPEENQIGEEGLGEVQ 120

Query: 121 LDKSFGYSKNFGAKFELGKEIGRGHFGHTFWAKGKRGDLKGKPVAVKIISKSKMTSAVGI 180
           LDKSFGYS NFGAKFELG+E+GRGHFGHTFWAKGK+GDLKGKPVAVKIISK+KMTS VGI
Sbjct: 121 LDKSFGYSTNFGAKFELGREVGRGHFGHTFWAKGKKGDLKGKPVAVKIISKTKMTSTVGI 180

Query: 181 EDVRREVKILKALSGHKNLVQFHDAFEDTNNIYMVMELCEGGELLERIVSRGGRYPEQES 240
           EDVRRE KILKALSGHKNLV FH AFED NNIY+VMELCEGGELLERIVSRGGRY EQES
Sbjct: 181 EDVRREEKILKALSGHKNLVHFHGAFEDANNIYIVMELCEGGELLERIVSRGGRYQEQES 240

Query: 241 KTIVVQILSVAAFCHLQGVVHRDLKPENFLFVNKDENTGLKVIDFGLSDFVKPDERLNDV 300
           KTI+VQIL V AFCHLQG+VHRDLKPENFLF+ KDENTGLK+IDFGLSDFVKPDERLNDV
Sbjct: 241 KTIIVQILCVVAFCHLQGIVHRDLKPENFLFMKKDENTGLKLIDFGLSDFVKPDERLNDV 300

Query: 301 VGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDD 360
           VGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCG RPFWARTESGIFRSVLRADPNFDD
Sbjct: 301 VGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGSRPFWARTESGIFRSVLRADPNFDD 360

Query: 361 SPWPTISAEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDESLAVPLDNFIYKSVKAYLRA 420
           SPWPTIS EAKDFVKRLLNKDHRKRMTAAQALTHPWLRDE LAVPLDNFIYKSVKAYLRA
Sbjct: 361 SPWPTISPEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDEDLAVPLDNFIYKSVKAYLRA 420

Query: 421 TPFKRAALKALAKALTEDELFYLRTQFKLLEPQNGFVALDNFKAALVRNATDAMRESNVA 480
           TPFKRAALKALAKALTEDELFYLRTQFKLLEPQNGFV +DN+KAALVRN TDAMRESNVA
Sbjct: 421 TPFKRAALKALAKALTEDELFYLRTQFKLLEPQNGFVTIDNYKAALVRNVTDAMRESNVA 480

Query: 481 DILKMMESLAYKRMDFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELV 540
           DILKMME LA KRM FEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELV
Sbjct: 481 DILKMMEPLANKRMGFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELV 540

Query: 541 QEMNLGPAAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRNSNTRH 586
           QEMNLGP AHSFLQNWTRSSDGKLSFFGYT+FLHGVTIR SNTRH
Sbjct: 541 QEMNLGPIAHSFLQNWTRSSDGKLSFFGYTKFLHGVTIRYSNTRH 571

BLAST of Tan0006333 vs. ExPASy TrEMBL
Match: A0A0A0KLT2 (Protein kinase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G139090 PE=4 SV=1)

HSP 1 Score: 1001.9 bits (2589), Expect = 1.1e-288
Identity = 503/585 (85.98%), Postives = 530/585 (90.60%), Query Frame = 0

Query: 1   MGHFCSKSVSDVNNDTAISGGTRPRSSHSGASPLPSGDAYSRPEESRQSYSVSTTASPFS 60
           MGH+CSK VSDV+N             H   S +P                  TT+SPFS
Sbjct: 1   MGHYCSKGVSDVSN-------------HPTISDVPQ----------------HTTSSPFS 60

Query: 61  SPLPAGIAPSPARTPGRKFRWPLPPPSPAKPIMAILRRKSRKASIPEDNQFGEEEVGEIQ 120
           SPLP GI PSPA TPGRKFRWPLPPPSPAKPIMAILRRKSRK SIPED++ GE+E+GE+Q
Sbjct: 61  SPLPPGIPPSPATTPGRKFRWPLPPPSPAKPIMAILRRKSRKPSIPEDDKIGEQELGEVQ 120

Query: 121 LDKSFGYSKNFGAKFELGKEIGRGHFGHTFWAKGKRGDLKGKPVAVKIISKSKMTSAVGI 180
           LDKSFGYS NF AKFELG E+GRGHFGHTFWAKGK+G+LKG PVAVKIISKSKMTSAVGI
Sbjct: 121 LDKSFGYSNNFAAKFELGMEVGRGHFGHTFWAKGKKGNLKGIPVAVKIISKSKMTSAVGI 180

Query: 181 EDVRREVKILKALSGHKNLVQFHDAFEDTNNIYMVMELCEGGELLERIVSRGGRYPEQES 240
           EDVR+EVKILKALSGH NLV FHDAFED NNIYMVMELCEGGELLERIVSRGG+YPEQES
Sbjct: 181 EDVRKEVKILKALSGHDNLVHFHDAFEDANNIYMVMELCEGGELLERIVSRGGKYPEQES 240

Query: 241 KTIVVQILSVAAFCHLQGVVHRDLKPENFLFVNKDENTGLKVIDFGLSDFVKPDERLNDV 300
           KTI+VQILSV AFCHLQGVVHRDLKPENFLF+ K+ENTGLKVIDFGLSDFVKPDERLNDV
Sbjct: 241 KTIIVQILSVVAFCHLQGVVHRDLKPENFLFLKKEENTGLKVIDFGLSDFVKPDERLNDV 300

Query: 301 VGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDD 360
           VGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDD
Sbjct: 301 VGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDD 360

Query: 361 SPWPTISAEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDESLAVPLDNFIYKSVKAYLRA 420
           SPWPTISAEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDE++AVPLDN IYKSVKAY+RA
Sbjct: 361 SPWPTISAEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDENIAVPLDNLIYKSVKAYVRA 420

Query: 421 TPFKRAALKALAKALTEDELFYLRTQFKLLEPQNGFVALDNFKAALVRNATDAMRESNVA 480
           TPFKRAALKALAKALTEDELFYLRTQFKLLEPQN FV LDNFKAALVRNATDAM+ES+VA
Sbjct: 421 TPFKRAALKALAKALTEDELFYLRTQFKLLEPQNRFVTLDNFKAALVRNATDAMKESHVA 480

Query: 481 DILKMMESLAYKRMDFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELV 540
           DILKMME LA ++MDFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELV
Sbjct: 481 DILKMMEPLAREKMDFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELV 540

Query: 541 QEMNLGPAAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRNSNTRH 586
           QEMNLGPAA+SFLQNWTRSSDGKLSFFG+TRFLHGVT+RNSNTRH
Sbjct: 541 QEMNLGPAAYSFLQNWTRSSDGKLSFFGFTRFLHGVTVRNSNTRH 556

BLAST of Tan0006333 vs. TAIR 10
Match: AT3G49370.1 (Calcium-dependent protein kinase (CDPK) family protein )

HSP 1 Score: 759.2 bits (1959), Expect = 2.4e-219
Identity = 383/592 (64.70%), Postives = 472/592 (79.73%), Query Frame = 0

Query: 1   MGHFCSKSVSDVNNDTAISGGTRPRSSHSGASPLPSGDAYSRPEESRQSYSVSTTASPFS 60
           MGH  S+++S V++D  I   T      S  +   +  + S P+    S       SPF 
Sbjct: 1   MGHCYSRNISTVDDDDEIPSATAQLPHRSHQNHHQTSSSSSIPQSPATSEVNPYNISPFQ 60

Query: 61  SPLPAGIAPSPARTPGRKFRWPLPPPSPAKPIMAILRRK------SRKASIPEDNQFGEE 120
           SPLPAG+APSPARTPGRKF+WP PPPSPAKPIMA LRR+       R   IPED++ G  
Sbjct: 61  SPLPAGVAPSPARTPGRKFKWPFPPPSPAKPIMAALRRRRGTAPHPRDGPIPEDSEAGGS 120

Query: 121 --EVGEIQLDKSFGYSKNFGAKFELGKEIGRGHFGHTFWAKGKRGDLKGKPVAVKIISKS 180
              +GE +LDK+FG++KNF  K+ELG+E+GRGHFGHT WAK K+G +KG+ VAVKIISKS
Sbjct: 121 GGGIGE-RLDKNFGFAKNFEGKYELGREVGRGHFGHTCWAKAKKGKIKGQTVAVKIISKS 180

Query: 181 KMTSAVGIEDVRREVKILKALSGHKNLVQFHDAFEDTNNIYMVMELCEGGELLERIVSRG 240
           KMTSA+ IEDVRREVK+LKALSGH ++V+F+D FED++N+++VMELCEGGELL+ I++RG
Sbjct: 181 KMTSALSIEDVRREVKLLKALSGHSHMVKFYDVFEDSDNVFVVMELCEGGELLDSILARG 240

Query: 241 GRYPEQESKTIVVQILSVAAFCHLQGVVHRDLKPENFLFVNKDENTGLKVIDFGLSDFVK 300
           GRYPE E+K I+VQILS  AF HLQGVVHRDLKPENFLF +K+E+  LKVIDFGLSD+ +
Sbjct: 241 GRYPEAEAKRILVQILSATAFFHLQGVVHRDLKPENFLFTSKNEDAVLKVIDFGLSDYAR 300

Query: 301 PDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGGRPFWARTESGIFRSVL 360
            D+RLNDVVGSAYYVAPEVL+RSYS EAD+WSIGVI+YILLCG RPF+ RTES IFR VL
Sbjct: 301 FDQRLNDVVGSAYYVAPEVLHRSYSTEADIWSIGVISYILLCGSRPFYGRTESAIFRCVL 360

Query: 361 RADPNFDDSPWPTISAEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDESLAVPLDNFIYK 420
           RA+PNFDD PWP+IS  AKDFVKRLLNKDHRKRMTAAQAL HPWLRDE+  + LD  IYK
Sbjct: 361 RANPNFDDLPWPSISPIAKDFVKRLLNKDHRKRMTAAQALAHPWLRDENPGLLLDFSIYK 420

Query: 421 SVKAYLRATPFKRAALKALAKALTEDELFYLRTQFKLLEPQNGFVALDNFKAALVRNATD 480
            VK+Y+RA+PF+RAALK+L+KA+ E+EL +L+ QF LLEP++G + L NF  AL R ATD
Sbjct: 421 LVKSYIRASPFRRAALKSLSKAIPEEELVFLKAQFMLLEPEDGGLHLHNFTTALTRYATD 480

Query: 481 AMRESNVADILKMMESLAYKRMDFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNR 540
           AM ES + DIL MM+ LA+K++DFEEFCAA++SVYQLEA+  WE IAT AFE+FE EG+R
Sbjct: 481 AMIESRLPDILNMMQPLAHKKLDFEEFCAASVSVYQLEALEEWEQIATVAFEHFESEGSR 540

Query: 541 VISVHELVQEMNLGPAAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRNSNTR 585
            ISV EL +EM+LGP A+  L++W RS DGKL+F GY +FLHGVT+R+S++R
Sbjct: 541 AISVQELAEEMSLGPNAYPLLKDWIRSLDGKLNFLGYAKFLHGVTVRSSSSR 591

BLAST of Tan0006333 vs. TAIR 10
Match: AT5G24430.1 (Calcium-dependent protein kinase (CDPK) family protein )

HSP 1 Score: 756.9 bits (1953), Expect = 1.2e-218
Identity = 382/598 (63.88%), Postives = 477/598 (79.77%), Query Frame = 0

Query: 1   MGHFCSKSVSDVNNDTAISG----GTRPRSSHSGASPLPSGDAYSRPEESRQSYSVSTTA 60
           MGH  S+++S V +D   +G      +P  +H  AS   S  A   PE +  SY++    
Sbjct: 1   MGHCYSRNISAVEDDEIPTGNGEVSNQPSQNHRHASIPQSPVASGTPEVN--SYNI---- 60

Query: 61  SPFSSPLPAGIAPSPARTPGRKFRWPLPPPSPAKPIMAILRRK------SRKASIPEDNQ 120
           SPF SPLPAG+APSPARTPGRKF+WP PPPSPAKPIMA LRR+       R   IPED++
Sbjct: 61  SPFQSPLPAGVAPSPARTPGRKFKWPFPPPSPAKPIMAALRRRRGAPPQPRDEPIPEDSE 120

Query: 121 ----FGEEEVGEIQLDKSFGYSKNFGAKFELGKEIGRGHFGHTFWAKGKRGDLKGKPVAV 180
                G +  G  +LDK+FG+ KNF  K+ELGKE+GRGHFGHT WAK K+G +K + VAV
Sbjct: 121 DVVDHGGDSGGGERLDKNFGFGKNFEGKYELGKEVGRGHFGHTCWAKAKKGKMKNQTVAV 180

Query: 181 KIISKSKMTSAVGIEDVRREVKILKALSGHKNLVQFHDAFEDTNNIYMVMELCEGGELLE 240
           KIISK+KMTS + IEDVRREVK+LKALSGH+++V+F+D +ED +N+++VMELCEGGELL+
Sbjct: 181 KIISKAKMTSTLSIEDVRREVKLLKALSGHRHMVKFYDVYEDADNVFVVMELCEGGELLD 240

Query: 241 RIVSRGGRYPEQESKTIVVQILSVAAFCHLQGVVHRDLKPENFLFVNKDENTGLKVIDFG 300
           RI++RGGRYPE ++K I+VQILS  AF HLQGVVHRDLKPENFLF +++E+  LKVIDFG
Sbjct: 241 RILARGGRYPEVDAKRILVQILSATAFFHLQGVVHRDLKPENFLFTSRNEDAILKVIDFG 300

Query: 301 LSDFVKPDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGGRPFWARTESG 360
           LSDF++ D+RLNDVVGSAYYVAPEVL+RSYS EADMWSIGVI+YILLCG RPF+ RTES 
Sbjct: 301 LSDFIRYDQRLNDVVGSAYYVAPEVLHRSYSTEADMWSIGVISYILLCGSRPFYGRTESA 360

Query: 361 IFRSVLRADPNFDDSPWPTISAEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDESLAVPL 420
           IFR VLRA+PNF+D PWP+IS  AKDFVKRLLNKDHRKRMTAAQAL HPWLRDE+  + L
Sbjct: 361 IFRCVLRANPNFEDMPWPSISPTAKDFVKRLLNKDHRKRMTAAQALAHPWLRDENPGLLL 420

Query: 421 DNFIYKSVKAYLRATPFKRAALKALAKALTEDELFYLRTQFKLLEPQNGFVALDNFKAAL 480
           D  +YK VK+Y+RA+PF+R+ALKAL+KA+ ++EL +L+ QF LL+P++G ++L+ F  AL
Sbjct: 421 DFSVYKLVKSYIRASPFRRSALKALSKAIPDEELVFLKAQFMLLDPKDGGLSLNCFTMAL 480

Query: 481 VRNATDAMRESNVADILKMMESLAYKRMDFEEFCAAAISVYQLEAVAGWESIATRAFEYF 540
            R ATDAM ES + DIL  M+ LA K++DFEEFCAAA+SVYQLEA+  WE IAT AFE+F
Sbjct: 481 TRYATDAMMESRLPDILNTMQPLAQKKLDFEEFCAAAVSVYQLEALEEWEQIATSAFEHF 540

Query: 541 EQEGNRVISVHELVQEMNLGPAAHSFLQNWTRSSDGKLSFFGYTRFLHGVTIRNSNTR 585
           E EGNR+ISV EL  EM++GP+A+  L++W RSSDGKLSF GY +FLHGVT+R+S++R
Sbjct: 541 EHEGNRIISVQELAGEMSVGPSAYPLLKDWIRSSDGKLSFLGYAKFLHGVTVRSSSSR 592

BLAST of Tan0006333 vs. TAIR 10
Match: AT2G46700.1 (CDPK-related kinase 3 )

HSP 1 Score: 740.7 bits (1911), Expect = 8.9e-214
Identity = 374/564 (66.31%), Postives = 461/564 (81.74%), Query Frame = 0

Query: 32  SPLPSGDAYSRPEESRQSYSVSTTASPF----SSPLPAGIAPSPAR--TPGRKFRWPLPP 91
           +P+  G A + P    +S + S   SPF    +SPLP+G++PSPAR  TP R FR P PP
Sbjct: 37  TPVNYGRAKNTP---ARSSNPSPWPSPFPHGSASPLPSGVSPSPARTSTPRRFFRRPFPP 96

Query: 92  PSPAKPIMAILRR----KSRKASIPEDNQFGEEEVGEIQLDKSFGYSKNFGAKFELGKEI 151
           PSPAK I A L +    K ++  IPE+   G E   E  LDKSFGY KNFGAK+ELGKE+
Sbjct: 97  PSPAKHIKASLIKRLGVKPKEGPIPEER--GTEP--EQSLDKSFGYGKNFGAKYELGKEV 156

Query: 152 GRGHFGHTFWAKGKRGDLKGKPVAVKIISKSKMTSAVGIEDVRREVKILKALSGHKNLVQ 211
           GRGHFGHT   +GK+GD+K  P+AVKIISK+KMT+A+ IEDVRREVK+LK+LSGHK L++
Sbjct: 157 GRGHFGHTCSGRGKKGDIKDHPIAVKIISKAKMTTAIAIEDVRREVKLLKSLSGHKYLIK 216

Query: 212 FHDAFEDTNNIYMVMELCEGGELLERIVSRGGRYPEQESKTIVVQILSVAAFCHLQGVVH 271
           ++DA ED NN+Y+VMELC+GGELL+RI++RGG+YPE ++K IVVQIL+V +FCHLQGVVH
Sbjct: 217 YYDACEDANNVYIVMELCDGGELLDRILARGGKYPEDDAKAIVVQILTVVSFCHLQGVVH 276

Query: 272 RDLKPENFLFVNKDENTGLKVIDFGLSDFVKPDERLNDVVGSAYYVAPEVLYRSYSFEAD 331
           RDLKPENFLF +  E++ LK+IDFGLSDF++PDERLND+VGSAYYVAPEVL+RSYS EAD
Sbjct: 277 RDLKPENFLFTSSREDSDLKLIDFGLSDFIRPDERLNDIVGSAYYVAPEVLHRSYSLEAD 336

Query: 332 MWSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDDSPWPTISAEAKDFVKRLLNKD 391
           +WSIGVI YILLCG RPFWARTESGIFR+VLR +PN+DD PWP+ S+E KDFVKRLLNKD
Sbjct: 337 IWSIGVITYILLCGSRPFWARTESGIFRTVLRTEPNYDDVPWPSCSSEGKDFVKRLLNKD 396

Query: 392 HRKRMTAAQALTHPWLRDESLAVPLDNFIYKSVKAYLRATPFKRAALKALAKALTEDELF 451
           +RKRM+A QALTHPWLRD+S  +PLD  IYK VKAYL ATP +RAALKALAKALTE+EL 
Sbjct: 397 YRKRMSAVQALTHPWLRDDSRVIPLDILIYKLVKAYLHATPLRRAALKALAKALTENELV 456

Query: 452 YLRTQFKLLEP-QNGFVALDNFKAALVRNATDAMRESNVADILKMMESLAYKRMDFEEFC 511
           YLR QF LL P ++G V+L+NFK AL++NATDAMRES V +IL  MESLAY++M FEEFC
Sbjct: 457 YLRAQFMLLGPNKDGSVSLENFKTALMQNATDAMRESRVPEILHTMESLAYRKMYFEEFC 516

Query: 512 AAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGPAAHSFLQNWTRSS 571
           AAAIS++QLEAV  WE IAT  F++FE EGNRVI++ EL +E+N+G +A+  L++W RSS
Sbjct: 517 AAAISIHQLEAVDAWEEIATAGFQHFETEGNRVITIEELARELNVGASAYGHLRDWVRSS 576

Query: 572 DGKLSFFGYTRFLHGVTIRNSNTR 585
           DGKLS+ G+T+FLHGVT+R ++ R
Sbjct: 577 DGKLSYLGFTKFLHGVTLRAAHAR 593

BLAST of Tan0006333 vs. TAIR 10
Match: AT3G50530.1 (CDPK-related kinase )

HSP 1 Score: 669.8 bits (1727), Expect = 1.9e-192
Identity = 336/543 (61.88%), Postives = 430/543 (79.19%), Query Frame = 0

Query: 58  PFSSPLPAGI-------APSPA-----RTPGRKFRWPLPPPSPAKPIMAILRR-----KS 117
           PF SP PA         A SPA      TP R F+ P PPPSPAK I A+L R     K 
Sbjct: 57  PFYSPSPAHYFFSKKTPARSPATNSTNSTPKRFFKRPFPPPSPAKHIRAVLARRHGSVKP 116

Query: 118 RKASIPEDNQFGEEEVGEIQLDKSFGYSKNFGAKFELGKEIGRGHFGHTFWAKGKRGDLK 177
             ++IPE +   E E G + LDKSFG+SK+F +K+ELG E+GRGHFG+T  AK K+GD K
Sbjct: 117 NSSAIPEGS---EAEGGGVGLDKSFGFSKSFASKYELGDEVGRGHFGYTCAAKFKKGDNK 176

Query: 178 GKPVAVKIISKSKMTSAVGIEDVRREVKILKALSGHKNLVQFHDAFEDTNNIYMVMELCE 237
           G+ VAVK+I K+KMT+A+ IEDVRREVKIL+ALSGH NL  F+DA+ED +N+Y+VMELCE
Sbjct: 177 GQQVAVKVIPKAKMTTAIAIEDVRREVKILRALSGHNNLPHFYDAYEDHDNVYIVMELCE 236

Query: 238 GGELLERIVSRGGRYPEQESKTIVVQILSVAAFCHLQGVVHRDLKPENFLFVNKDENTGL 297
           GGELL+RI+SRGG+Y E+++KT+++QIL+V AFCHLQGVVHRDLKPENFLF +K++ + L
Sbjct: 237 GGELLDRILSRGGKYTEEDAKTVMIQILNVVAFCHLQGVVHRDLKPENFLFTSKEDTSQL 296

Query: 298 KVIDFGLSDFVKPDERLNDVVGSAYYVAPEVLYRSYSFEADMWSIGVIAYILLCGGRPFW 357
           K IDFGLSD+V+PDERLND+VGSAYYVAPEVL+RSYS EAD+WS+GVI YILLCG RPFW
Sbjct: 297 KAIDFGLSDYVRPDERLNDIVGSAYYVAPEVLHRSYSTEADIWSVGVIVYILLCGSRPFW 356

Query: 358 ARTESGIFRSVLRADPNFDDSPWPTISAEAKDFVKRLLNKDHRKRMTAAQALTHPWLRDE 417
           ARTESGIFR+VL+ADP+FDD PWP +S+EA+DFVKRLLNKD RKR+TAAQAL+HPW++D 
Sbjct: 357 ARTESGIFRAVLKADPSFDDPPWPLLSSEARDFVKRLLNKDPRKRLTAAQALSHPWIKDS 416

Query: 418 SLA-VPLDNFIYKSVKAYLRATPFKRAALKALAKALTEDELFYLRTQFKLLEP-QNGFVA 477
           + A VP+D  ++K ++AYLR++  ++AAL+AL+K LT DELFYLR QF LLEP +NG ++
Sbjct: 417 NDAKVPMDILVFKLMRAYLRSSSLRKAALRALSKTLTVDELFYLREQFALLEPSKNGTIS 476

Query: 478 LDNFKAALVRNATDAMRESNVADILKMMESLAYKRMDFEEFCAAAISVYQLEAVAGWESI 537
           L+N K+AL++ ATDAM++S + + L  + +L Y+RMDFEEFCAAA+SV+QLEA+  WE  
Sbjct: 477 LENIKSALMKMATDAMKDSRIPEFLGQLSALQYRRMDFEEFCAAALSVHQLEALDRWEQH 536

Query: 538 ATRAFEYFEQEGNRVISVHELVQEMNLGPA--AHSFLQNWTRSSDGKLSFFGYTRFLHGV 580
           A  A+E FE+EGNR I + EL  E+ LGP+   H+ L +W R +DGKLSF G+ + LHGV
Sbjct: 537 ARCAYELFEKEGNRPIMIDELASELGLGPSVPVHAVLHDWLRHTDGKLSFLGFVKLLHGV 596

BLAST of Tan0006333 vs. TAIR 10
Match: AT2G41140.1 (CDPK-related kinase 1 )

HSP 1 Score: 654.8 bits (1688), Expect = 6.4e-188
Identity = 336/574 (58.54%), Postives = 427/574 (74.39%), Query Frame = 0

Query: 23  RPRSSHSGASPLPSGDAYSRPEESRQSYSVSTTASPFSSPLPA--------GIAPSPART 82
           +P    S + P+ SG+    P  S+      ++  PF SP P          ++ S + T
Sbjct: 7   KPVEQQSKSLPV-SGETNEAPTNSQP--PAKSSGFPFYSPSPVPSLFKSSPSVSSSVSST 66

Query: 83  PGRKFRWPLPPPSPAKPIMAILRR-----KSRKASIPEDNQFGEEEVGEIQLDKSFGYSK 142
           P R F+ P PPPSPAK I A L R     K  + SIPE  +       EI LDKSFG+SK
Sbjct: 67  PLRIFKRPFPPPSPAKHIRAFLARRYGSVKPNEVSIPEGKEC------EIGLDKSFGFSK 126

Query: 143 NFGAKFELGKEIGRGHFGHTFWAKGKRGDLKGKPVAVKIISKSKMTSAVGIEDVRREVKI 202
            F + +E+  E+GRGHFG+T  AKGK+G LKG+ VAVK+I KSKMT+A+ IEDV REVK+
Sbjct: 127 QFASHYEIDGEVGRGHFGYTCSAKGKKGSLKGQEVAVKVIPKSKMTTAIAIEDVSREVKM 186

Query: 203 LKALSGHKNLVQFHDAFEDTNNIYMVMELCEGGELLERIVSRGGRYPEQESKTIVVQILS 262
           L+AL+GHKNLVQF+DAFED  N+Y+VMELC+GGELL++I+ RGG+Y E ++K ++VQILS
Sbjct: 187 LRALTGHKNLVQFYDAFEDDENVYIVMELCKGGELLDKILQRGGKYSEDDAKKVMVQILS 246

Query: 263 VAAFCHLQGVVHRDLKPENFLFVNKDENTGLKVIDFGLSDFVKPDERLNDVVGSAYYVAP 322
           V A+CHLQGVVHRDLKPENFLF  KDE + LK IDFGLSD+VKPDERLND+VGSAYYVAP
Sbjct: 247 VVAYCHLQGVVHRDLKPENFLFSTKDETSPLKAIDFGLSDYVKPDERLNDIVGSAYYVAP 306

Query: 323 EVLYRSYSFEADMWSIGVIAYILLCGGRPFWARTESGIFRSVLRADPNFDDSPWPTISAE 382
           EVL+R+Y  EADMWSIGVIAYILLCG RPFWARTESGIFR+VL+A+PNF+++PWP++S E
Sbjct: 307 EVLHRTYGTEADMWSIGVIAYILLCGSRPFWARTESGIFRAVLKAEPNFEEAPWPSLSPE 366

Query: 383 AKDFVKRLLNKDHRKRMTAAQALTHPWL-RDESLAVPLDNFIYKSVKAYLRATPFKRAAL 442
           A DFVKRLLNKD+RKR+TAAQAL HPWL     L +P D  IYK VK Y+ +T  +++AL
Sbjct: 367 AVDFVKRLLNKDYRKRLTAAQALCHPWLVGSHELKIPSDMIIYKLVKVYIMSTSLRKSAL 426

Query: 443 KALAKALTEDELFYLRTQFKLLEP-QNGFVALDNFKAALVRNATDAMRESNVADILKMME 502
            ALAK LT  +L YLR QF LL P +NG++++ N+K A+++++TDAM++S V D + M+ 
Sbjct: 427 AALAKTLTVPQLAYLREQFTLLGPSKNGYISMQNYKTAILKSSTDAMKDSRVFDFVHMIS 486

Query: 503 SLAYKRMDFEEFCAAAISVYQLEAVAGWESIATRAFEYFEQEGNRVISVHELVQEMNLGP 562
            L YK++DFEEFCA+A+SVYQLEA+  WE  A RA+E FE++GNR I + EL  E+ LGP
Sbjct: 487 CLQYKKLDFEEFCASALSVYQLEAMETWEQHARRAYELFEKDGNRPIMIEELASELGLGP 546

Query: 563 A--AHSFLQNWTRSSDGKLSFFGYTRFLHGVTIR 580
           +   H  LQ+W R SDGKLSF G+ R LHGV+ R
Sbjct: 547 SVPVHVVLQDWIRHSDGKLSFLGFVRLLHGVSSR 571