Tan0005919 (gene) Snake gourd v1

BLAST of Tan0005919 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 893.6 bits (2308), Expect = 1.4e-258
Identity = 456/735 (62.04%), Postives = 558/735 (75.92%), Query Frame = 0

Query: 8   MSSSLIFILVFLNL-ISNLLASSSDSDNDGRKIYIVYLGNKPEDSASTPSHHMRMLEEVV 67
           MSSSLIF L F +L  SN LAS  DSD+DG+ IYIVY+G K ED  S   HH  MLE+VV
Sbjct: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV 60

Query: 68  GSKFAPKSLLHSYKRSFNGFVVRLTEEEAQKISGKKGVVSVFPNEKKHLHTTRSWDFMGF 127
           GS FAP+S+LH+YKRSFNGF V+LTEEEA+KI+  +GVVSVF NE   LHTTRSWDF+GF
Sbjct: 61  GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF 120

Query: 128 TKNVPRVNQVESNIVVGVLDSGIWPESPSFSDVGFGLPPTKWKGTCQTSANFRCNRKIIG 187
              VPR +QVESNIVVGVLD+GIWPESPSF D GF  PP KWKGTC+TS NFRCNRKIIG
Sbjct: 121 PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIG 180

Query: 188 ARAYRSDNFFPPEDIEGPRDSDGHGTHTASTVAGGLVDQASLFGLGLGTARGG------- 247
           AR+Y       P D+ GPRD++GHGTHTAST AGGLV QA+L+GLGLGTARGG       
Sbjct: 181 ARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIA 240

Query: 248 ---VCWSDGCHDADILAAFDDAIADGVDIISLSVGGNEPRFYFNDSIAIGAFHSMKHGIL 307
              VCW+DGC D DILAA+DDAIADGVDIISLSVGG  PR YF D+IAIG+FH+++ GIL
Sbjct: 241 AYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGIL 300

Query: 308 TSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSKLQLGNSKIYQGYTINTFDLLGKQ 367
           TSNSAGN GP++FT  + SPW LSVAAST+DRK V+++Q+GN + +QG +INTFD   + 
Sbjct: 301 TSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD--NQY 360

Query: 368 YPLIYAGDALNVTGGFTGLSSR----KFSGSQSGEGKILLCDSILAPSTFASFSDAVGVV 427
           YPL+   D  N   GF   +SR    K       +GKI++C++   P  F    D    V
Sbjct: 361 YPLVSGRDIPNT--GFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGV 420

Query: 428 MNDAGVKDNSRSYPLPSSYLETADGDSIKSYMASNGVATVTIFKSDAVNDSSAPLIVSFS 487
           +  +  +D + SYPLPSS L+  D  +   Y+ S      TIFKS  + ++SAP++VSFS
Sbjct: 421 LMTSNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFS 480

Query: 488 SRGPNPETLDILKPDLTAPGVEILAAWSPNASVSSGVRDSRKTMYNIISGTSMSCPHATA 547
           SRGPN  T D++KPD++ PGVEILAAW P+ +   G+R  R T++NIISGTSMSCPH T 
Sbjct: 481 SRGPNRATKDVIKPDISGPGVEILAAW-PSVAPVGGIR--RNTLFNIISGTSMSCPHITG 540

Query: 548 AAVYVKTFHPTWSPAAIKSALMTTAIPLNAELNPEAEFAYGAGHIDPLKAINPGLVYDAN 607
            A YVKT++PTWSPAAIKSALMTTA P+NA  NP+AEFAYG+GH++PLKA+ PGLVYDAN
Sbjct: 541 IATYVKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDAN 600

Query: 608 ESDYVKFLCGQGYSTAMVRRLSGDGSVCTLANSGRVWDLNYPSFALSTAPSESINQFFRR 667
           ESDYVKFLCGQGY+T  VRR++GD S CT  N+GRVWDLNYPSF LS +PS++ NQ+F R
Sbjct: 601 ESDYVKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQTFNQYFNR 660

Query: 668 TLTNVESKASTYRYKVLGAPQGLTITVDPPILSFNGIGQKESFTLTIRGTVRQSIVSASL 727
           TLT+V  +ASTYR  ++ APQGLTI+V+P +LSFNG+G ++SFTLT+RG+++  +VSASL
Sbjct: 661 TLTSVAPQASTYR-AMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASL 720

BLAST of Tan0005919 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 623.6 bits (1607), Expect = 2.8e-177
Identity = 333/717 (46.44%), Postives = 458/717 (63.88%), Query Frame = 0

Query: 32  SDNDGRK---IYIVYLGNKPEDSASTPSHHMRMLEEVVGSKFAPKSLLHSYKRSFNGFVV 91
           S ND R+   +YIVY+G  PE   S PSHH+ +L+++VG+  A   L+ SYKRSFNGF  
Sbjct: 22  SANDYRQASSVYIVYMGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAA 81

Query: 92  RLTEEEAQKISGKKGVVSVFPNEKKHLHTTRSWDFMGFTKNVPRVNQVESNIVVGVLDSG 151
            L++ E+QK+   K VVSVFP++   L TTRSWDF+GF +   R +  ES+++VGV+DSG
Sbjct: 82  NLSQAESQKLQNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSG 141

Query: 152 IWPESPSFSDVGFGLPPTKWKGTCQTSANFRCNRKIIGARAYRSDNFFPPEDIEGPRDSD 211
           IWPES SF D GFG PP KWKG+C+    F CN K+IGAR Y        +  +  RD +
Sbjct: 142 IWPESESFDDEGFGPPPKKWKGSCKGGLKFACNNKLIGARFYN-------KFADSARDEE 201

Query: 212 GHGTHTASTVAGGLVDQASLFGLGLGTARGG----------VCWSDGCHDADILAAFDDA 271
           GHGTHTAST AG  V  AS +GL  GTARGG          VC+ + C+D DILAAFDDA
Sbjct: 202 GHGTHTASTAAGNAVQAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDA 261

Query: 272 IADGVDIISLSVGGNEPRFYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWS 331
           IADGVD+IS+S+  +      N S+AIG+FH+M  GI+T+ SAGN+GPD  ++ N SPW 
Sbjct: 262 IADGVDVISISISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWM 321

Query: 332 LSVAASTIDRKLVSKLQLGNSKIYQGYTINTFDLLGKQYPLIYAGD-ALNVTGGFTGLSS 391
           ++VAAS  DR+ + ++ LGN K   G ++NTF+L G ++P++Y  + + N +    G  S
Sbjct: 322 ITVAASGTDRQFIDRVVLGNGKALTGISVNTFNLNGTKFPIVYGQNVSRNCSQAQAGYCS 381

Query: 392 RKFSGSQSGEGKILLCDSILAPSTFASFSDAVGVVMNDAGVKDNSRSYPLPSSYLETADG 451
                S+  +GKI+LCD  L     A  + A+GV++ +  + D++   P P+S L   D 
Sbjct: 382 SGCVDSELVKGKIVLCDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDY 441

Query: 452 DSIKSYMASNGVATVTIFKSDAVNDSSAPLIVSFSSRGPNPETLDILKPDLTAPGVEILA 511
            SIKSY+ S       I +++ + D  AP + SFSSRGP+    ++LKPD++APG+EILA
Sbjct: 442 KSIKSYIESAEPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILA 501

Query: 512 AWSPNASVSS--GVRDSRKTMYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMT 571
           A+SP AS SS     D R   Y+++SGTSM+CPH    A YVK+FHP WSP+AIKSA+MT
Sbjct: 502 AFSPVASPSSFLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMT 561

Query: 572 TAIPLNAELNPEAEFAYGAGHIDPLKAINPGLVYDANESDYVKFLCGQGYSTAMVRRLSG 631
           TA P+N + NPE EFAYG+G I+P KA +PGLVY+    DY+K LC +G+ +  +   SG
Sbjct: 562 TATPMNLKKNPEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSG 621

Query: 632 DGSVCTLANSGRVWDLNYPSFALSTAPSESINQFFRRTLTNVESKASTYRYKVLGAPQGL 691
               C  +    V DLNYP+     +  +  N  F+RT+TNV    STY+  V+     L
Sbjct: 622 QNVTC--SERTEVKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPEL 681

Query: 692 TITVDPPILSFNGIGQKESFTLTIRGTVRQ--SIVSASLVWTDGSHNVRSPITVYVV 731
            I+++P IL F  + +K+SF +TI G   +  S VS+S+VW+DGSH+VRSPI  Y +
Sbjct: 682 QISIEPEILRFGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSI 727

BLAST of Tan0005919 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 1.2e-172
Identity = 344/741 (46.42%), Postives = 458/741 (61.81%), Query Frame = 0

Query: 9   SSSLIFILVFLNLISNLLASSSDSDNDGRKIYIVYLGNKPEDSASTP-SHHMRMLEEVVG 68
           SSSL+  L+ L L      SS  +  D +++YIVY+G+    +  TP S HM +L+EV G
Sbjct: 7   SSSLLSCLLVLFL------SSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTG 66

Query: 69  SKFAPKSLLHSYKRSFNGFVVRLTEEEAQKISGKKGVVSVFPNEKKHLHTTRSWDFMGFT 128
                  L+ SYKRSFNGF  RLTE E ++++   GVVSVFPN+K  L TT SWDFMG  
Sbjct: 67  ESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLK 126

Query: 129 KNV--PRVNQVESNIVVGVLDSGIWPESPSFSDVGFGLPPTKWKGTCQTSANFRCNRKII 188
           + +   R   VES+ ++GV+DSGI PES SFSD GFG PP KWKG C    NF CN K+I
Sbjct: 127 EGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLI 186

Query: 189 GARAYRSDNFFPPEDIEGPRDSDGHGTHTASTVAGGLVDQASLFGLGLGTARGG------ 248
           GAR Y S         EG RD DGHGTHTAST AG  V  AS FG+G GT RGG      
Sbjct: 187 GARDYTS---------EGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRV 246

Query: 249 ----VCWSDGCHDADILAAFDDAIADGVDIISLSVGGNEPRFYFNDSIAIGAFHSMKHGI 308
               VC   GC    +L+AFDDAIADGVD+I++S+G      + ND IAIGAFH+M  G+
Sbjct: 247 AAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGV 306

Query: 309 LTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSKLQLGNSKIYQGYTINTFDLLGK 368
           LT NSAGN GP   ++   +PW L+VAAST +R  V+K+ LGN K   G ++N +++ GK
Sbjct: 307 LTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGK 366

Query: 369 QYPLIYAGDALN--VTGGFTGLSSRKFSGSQSGEGKILLCDSILAPSTFASFSDAVGVVM 428
            YPL+Y   A +        GL           +GKIL+C          S   AVG++ 
Sbjct: 367 DYPLVYGKSAASSACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESVG-AVGLIY 426

Query: 429 NDAGVKDNSRSYPLPSSYLETADGDSIKSYMASNGVATVTIFKSDAVNDSSAPLIVSFSS 488
                 D +  +PLP++ L T D +S+ SY+ S       + K++A+ + ++P+I SFSS
Sbjct: 427 R-TPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSS 486

Query: 489 RGPNPETLDILKPDLTAPGVEILAAWSPNASVSSGVRDSRKTMYNIISGTSMSCPHATAA 548
           RGPN   +DILKPD+TAPGVEILAA+SP    S    D+R   Y+++SGTSMSCPH    
Sbjct: 487 RGPNTIAVDILKPDITAPGVEILAAYSPAGEPSQD--DTRHVKYSVLSGTSMSCPHVAGV 546

Query: 549 AVYVKTFHPTWSPAAIKSALMTTAIPLNAELN--PEAEFAYGAGHIDPLKAINPGLVYDA 608
           A YVKTF+P WSP+ I+SA+MTTA P+NA        EFAYG+GH+DP+ A NPGLVY+ 
Sbjct: 547 AAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYEL 606

Query: 609 NESDYVKFLCGQGYSTAMVRRLSGDGSVCTLANSGRVWDLNYPSFALSTAPS-ESINQFF 668
           ++SD++ FLCG  Y++ +++ +SG+   C+ A      +LNYPS +   + S  +    F
Sbjct: 607 DKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTF 666

Query: 669 RRTLTNVESKASTYRYKVL-GAPQGLTITVDPPILSFNGIGQKESFTLTIRGTVRQSIV- 728
            RTLTNV +  STY  KV+ G    L + + P +LSF  + +K+SFT+T+ G+   S V 
Sbjct: 667 NRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVP 726

BLAST of Tan0005919 vs. ExPASy Swiss-Prot
Match: Q9FGU3 (Subtilisin-like protease SBT4.4 OS=Arabidopsis thaliana OX=3702 GN=SBT4.4 PE=2 SV=1)

HSP 1 Score: 607.1 bits (1564), Expect = 2.7e-172
Identity = 336/746 (45.04%), Postives = 470/746 (63.00%), Query Frame = 0

Query: 8   MSSSLIFILVFLNLISNLLAS-SSDSDNDG-RKIYIVYLGNKPEDSASTP-SHHMRMLEE 67
           M+    FI +F +L+   L+S S+D D+ G +++YIVYLG+ P     TP S HM +L+E
Sbjct: 1   MAKGTTFIFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQE 60

Query: 68  VVGSKFAPKSLLHSYKRSFNGFVVRLTEEEAQKISGKKGVVSVFPNEKKHLHTTRSWDFM 127
           + G       L+ SYK+SFNGF  RLTE E ++++G + VVSVFP+ K  L TT SW+FM
Sbjct: 61  ITGESLIENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFM 120

Query: 128 GFTKNV--PRVNQVESNIVVGVLDSGIWPESPSFSDVGFGLPPTKWKGTCQTSANFRCNR 187
           G  + +   R   +ES+ ++GV+DSGI+PES SFSD GFG PP KWKGTC    NF CN 
Sbjct: 121 GLKEGIKTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNN 180

Query: 188 KIIGARAYRSDNFFPPEDIEGPRDSDGHGTHTASTVAGGLVDQASLFGLGLGTARGG--- 247
           K+IGAR Y + +    +  +  RD  GHGTHTAS  AG  V  ++ +GLG GTARGG   
Sbjct: 181 KVIGARDYTAKS----KANQTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPA 240

Query: 248 -------VCWSDGCHDADILAAFDDAIADGVDIISLSVGGNEPRFYFNDSIAIGAFHSMK 307
                  VC ++GC    +++AFDDAIADGVD+IS+S+  +    +  D IAIGAFH+M 
Sbjct: 241 ARIAVYKVCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMA 300

Query: 308 HGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSKLQLGNSKIYQGYTINTFDL 367
            G+LT N+AGN+GP   T+ + +PW  SVAAS  +R  ++K+ LG+ KI  G ++NT+D+
Sbjct: 301 VGVLTVNAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDM 360

Query: 368 LGKQYPLIYAGDALNVTGGF--TGLSSRKFSGSQSGEGKILLCDSILAPSTFASFSDAVG 427
            G  YPL+Y   A   T       L   K    +  +GKI+LCDS               
Sbjct: 361 NGTNYPLVYGKSAALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVGS 420

Query: 428 VVMNDAGVKDNSRSYPLPSSYLETADGDSIKSYMASNGVATVTIFKSDAVNDSSAPLIVS 487
           +V N    +   RS+P+  S+L   D  S+ SYM S      T+ KS+ +++  APL+ S
Sbjct: 421 IVKNPEPDRAFIRSFPV--SFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVAS 480

Query: 488 FSSRGPNPETLDILKPDLTAPGVEILAAWSPNASVSSGVRDSRKTMYNIISGTSMSCPHA 547
           FSSRGP+    DILKPD+TAPGVEILAA+SP++S +    D+R+  Y+++SGTSM+CPH 
Sbjct: 481 FSSRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHV 540

Query: 548 TAAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELN--PEAEFAYGAGHIDPLKAINPGLV 607
              A YVKTFHP WSP+ I+SA+MTTA P+NA  +     EFAYG+GH+DP+ AINPGLV
Sbjct: 541 AGVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLV 600

Query: 608 YDANESDYVKFLCGQGYSTAMVRRLSGDGSVCTLANSGRV-WDLNYPSFALSTAPSESIN 667
           Y+  ++D++ FLCG  Y++  +R +SGD S CT   S  +  +LNYP+ +   + ++  N
Sbjct: 601 YELTKADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFN 660

Query: 668 QFFRRTLTNVESKASTYRYKVLGAP-QGLTITVDPPILSFNGIGQKESFTLTIRGTV--R 727
             F+RT+TNV  + STY  KV+  P   L+I V P +LS   + +K+SF +T+       
Sbjct: 661 ITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGT 720

Query: 728 QSIVSASLVWTDGSHNVRSPITVYVV 731
           +  VSA+L+W+DG+HNVRSPI VY +
Sbjct: 721 KQPVSANLIWSDGTHNVRSPIIVYAM 740

BLAST of Tan0005919 vs. ExPASy Swiss-Prot
Match: Q9FIM6 (Subtilisin-like protease SBT4.8 OS=Arabidopsis thaliana OX=3702 GN=SBT4.8 PE=3 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 6.0e-172
Identity = 343/737 (46.54%), Postives = 464/737 (62.96%), Query Frame = 0

Query: 15  ILVFLNLISNLLASSSDSDNDGRKIYIVYLGNKPEDSASTP-SHHMRMLEEVVGSKFAPK 74
           I++FL+ +S ++    D     +++Y+VY+G+ P     TP S+H+ +L+EV G      
Sbjct: 14  IILFLSSVSAIIYDPQD-----KQVYVVYMGSLPSQPNYTPMSNHINILQEVTGESSIEG 73

Query: 75  SLLHSYKRSFNGFVVRLTEEEAQKISGKKGVVSVFPNEKKHLHTTRSWDFMGFT--KNVP 134
            L+ SYKRSFNGF   LTE E + ++  +GVVSVF ++   L TT SWDFMG    KN  
Sbjct: 74  RLVRSYKRSFNGFSALLTESEREGVAEMEGVVSVFRSKNYKLQTTASWDFMGMKEGKNTK 133

Query: 135 RVNQVESNIVVGVLDSGIWPESPSFSDVGFGLPPTKWKGTCQTSANFRCNRKIIGARAYR 194
           R   VES+ ++G +DSGIWPES SFSD GFG PP KWKG C+   NF CN K+IGAR Y 
Sbjct: 134 RNFAVESDTIIGFIDSGIWPESESFSDKGFGPPPKKWKGVCKGGKNFTCNNKLIGARDYT 193

Query: 195 SDNFFPPEDIEGPRDSDGHGTHTASTVAGGLVDQASLFGLGLGTARGG----------VC 254
           S         EG RD  GHGTHT ST AG  V   S FG+G GTARGG          VC
Sbjct: 194 S---------EGTRDLQGHGTHTTSTAAGNAVADTSFFGIGNGTARGGVPASRVAAYKVC 253

Query: 255 WSDGCHDADILAAFDDAIADGVDIISLSVGGNEPRFYFNDSIAIGAFHSMKHGILTSNSA 314
              GC D ++L+AFDDAIADGVD+IS+S+GG+ P  Y  D+IAIGAFH+M  GILT +SA
Sbjct: 254 TITGCSDDNVLSAFDDAIADGVDLISVSLGGDYPSLYAEDTIAIGAFHAMAKGILTVHSA 313

Query: 315 GNDGPDYFTIRNFSPWSLSVAASTIDRKLVSKLQLGNSKIYQGYTINTFDLLGKQYPLIY 374
           GN GP+  T+ + +PW L+VAA+T +R+ ++K+ LGN K   G ++N FDL GK+YPL Y
Sbjct: 314 GNAGPNPTTVVSVAPWMLTVAATTTNRRFLTKVVLGNGKTLVGKSVNAFDLKGKKYPLEY 373

Query: 375 AGDALNVTGGFTGLSSRKFSGSQSGEGKILLCDSILAPSTFASFSD-AVGVVMNDAGVKD 434
            GD LN                   +GKIL+       S + S S+ AV  +  D   KD
Sbjct: 374 -GDYLN---------------ESLVKGKILV-------SRYLSGSEVAVSFITTDN--KD 433

Query: 435 NSRSYPLPSSYLETADGDSIKSYMASNGVATVTIFKSDAVNDSSAPLIVSFSSRGPNPET 494
            +     P S L   D DS+ SY+ S      ++ K++A+ +  +P + SFSSRGPN   
Sbjct: 434 YASISSRPLSVLSQDDFDSLVSYINSTRSPQGSVLKTEAIFNQLSPKVASFSSRGPNTIA 493

Query: 495 LDILKPDLTAPGVEILAAWSPNASVSSGVRDSRKTMYNIISGTSMSCPHATAAAVYVKTF 554
           +DILKPD++APGVEILAA+SP +  S   RD R+  Y+++SGTSM+CPH T  A Y+KTF
Sbjct: 494 VDILKPDISAPGVEILAAYSPLSLPSEDRRDKRRVKYSVLSGTSMACPHVTGVAAYIKTF 553

Query: 555 HPTWSPAAIKSALMTTAIPLNAELN--PEAEFAYGAGHIDPLKAINPGLVYDANESDYVK 614
           HP WSP+ I+SA+MTTA  +NA        EFAYGAGH+DP+ AINPGLVY+ N++D++ 
Sbjct: 554 HPDWSPSVIQSAIMTTAWQMNATGTGAESTEFAYGAGHVDPIAAINPGLVYELNKTDHIS 613

Query: 615 FLCGQGYSTAMVRRLSGDGSVCTLANSGRVWDLNYPSFALSTAPSESINQF---FRRTLT 674
           FLCG  Y++  ++ +SGD  +C+     R  +LNYPS  +S   SES + F   F+RT+T
Sbjct: 614 FLCGMNYTSKTLKLISGDAVICSGKTLQR--NLNYPS--MSAKLSESNSSFTVTFKRTVT 673

Query: 675 NVESKASTYRYK-VLGAPQGLTITVDPPILSFNGIGQKESFTLTIRGTVRQSIV--SASL 730
           N+ +  STY+ K VL     L + V P +LS   + +K+SFT+T+ G+     +  SA+L
Sbjct: 674 NLGTANSTYKSKIVLNHGSKLNVKVSPSVLSMKSLKEKQSFTVTVSGSNIDPKLPSSANL 707

BLAST of Tan0005919 vs. NCBI nr
Match: XP_023536304.1 (cucumisin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1220.7 bits (3157), Expect = 0.0e+00
Identity = 614/741 (82.86%), Postives = 668/741 (90.15%), Query Frame = 0

Query: 8   MSSSLIF-ILVFLNLISNLLASSSD-SDNDGRKIYIVYLGNKPEDSASTPSHHMRMLEEV 67
           MSSSLIF +++F++   +LLASS D SDNDGRK+YIVYLGNKPEDSASTPSHHMRMLEEV
Sbjct: 1   MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60

Query: 68  VGSKFAPKSLLHSYKRSFNGFVVRLTEEEAQKISGKKGVVSVFPNEKKHLHTTRSWDFMG 127
           VGS FAP +LLHSYKRSFNGFVV+LTEEEAQKIS K+GVVSVFPN KKHLHTTRSWDFMG
Sbjct: 61  VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120

Query: 128 FTKNVPRVNQVESNIVVGVLDSGIWPESPSFSDVGFGLPPTKWKGTCQTSANFRCNRKII 187
           FTKNV RV QVESNIVVGVLDSGIWPESPSFSDVG+GLPP KWKG CQTSANFRCNRKI+
Sbjct: 121 FTKNVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGLPPAKWKGACQTSANFRCNRKIV 180

Query: 188 GARAYRSDNFFPPEDIEGPRDSDGHGTHTASTVAGGLVDQASLFGLGLGTARGGV----- 247
           GARAYRSD  FPPEDI+ PRDSDGHGTHTASTVAGGLV+QASL+GL LGTARGGV     
Sbjct: 181 GARAYRSDKSFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240

Query: 248 -----CWSDGCHDADILAAFDDAIADGVDIISLSVGGNEPRFYFNDSIAIGAFHSMKHGI 307
                CWSDGC+DADILAAFDDAIADGVDIISLSVGGN+P++YFNDSIAIGAFHSMKHGI
Sbjct: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300

Query: 308 LTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSKLQLGNSKIYQGYTINTFDLLGK 367
           LTSNSAGNDGPDYFTIRNFSPWSLSVAAS+IDRKLVSK+QLGN  +YQGYTINTFDL GK
Sbjct: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360

Query: 368 QYPLIYAGDALNVTGGFTGLSSRKFSGSQSG----EGKILLCDSILAPSTFASFSDAVGV 427
           QYPLIYAG+A NV+GGFTG SSR  S +        GKILLCDSIL+PSTFASFS AVGV
Sbjct: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFSGAVGV 420

Query: 428 VMNDAGVKDNSRSYPLPSSYLETADGDSIKSYMASNGVATVTIFKSDAVNDSSAPLIVSF 487
           VMNDAGVKDN+RSYPLPSSYL    G++IK+YM SN   T TIFKS+ VND+SAPLIVSF
Sbjct: 421 VMNDAGVKDNARSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNTVNDTSAPLIVSF 480

Query: 488 SSRGPNPETLDILKPDLTAPGVEILAAWSPNASVSSGVRDSRKTMYNIISGTSMSCPHAT 547
           SSRGPNPET DILKPDLTAPGVEILAAWSP ASVSSGVRDSR T+YNIISGTSMSCPHAT
Sbjct: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVRDSRTTLYNIISGTSMSCPHAT 540

Query: 548 AAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELNPEAEFAYGAGHIDPLKAINPGLVYDA 607
           AAAVYVKTFHP+WSPAAIKSALMTTAIPLN +LNP+AEFAYGAGHI+P+KA+NPGLVYDA
Sbjct: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600

Query: 608 NESDYVKFLCGQGYSTAMVRRLSGDGSVCTLANSGRVWDLNYPSFALSTAPSESINQFFR 667
            ESDYV+FLCGQGY+TAMVRRLSGD SVCT ANSGRVWDLNYPSFALS+ PSESINQFFR
Sbjct: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660

Query: 668 RTLTNVESKASTYRYKVLGAPQGLTITVDPPILSFNGIGQKESFTLTIRGTVRQSIVSAS 727
           RT+TNV SK +TYR KVLG P+GLTI+V+PP+LSFN IGQK+SFT+T+RG V Q IVSA+
Sbjct: 661 RTVTNVGSKVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGGVNQPIVSAA 720

Query: 728 LVWTDGSHNVRSPITVYVVIK 733
           L+WTDG H+VRSPITVYVV K
Sbjct: 721 LLWTDGHHHVRSPITVYVVDK 741

BLAST of Tan0005919 vs. NCBI nr
Match: XP_022979186.1 (cucumisin-like [Cucurbita maxima])

HSP 1 Score: 1218.8 bits (3152), Expect = 0.0e+00
Identity = 615/741 (83.00%), Postives = 668/741 (90.15%), Query Frame = 0

Query: 8   MSSSLIF-ILVFLNLISNLLASSSD-SDNDGRKIYIVYLGNKPEDSASTPSHHMRMLEEV 67
           MSSSLIF +++F++   +LLASS D SDNDGRK+YIVYLGNKPEDSASTPSHHMRMLEEV
Sbjct: 1   MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60

Query: 68  VGSKFAPKSLLHSYKRSFNGFVVRLTEEEAQKISGKKGVVSVFPNEKKHLHTTRSWDFMG 127
           VGS FAP +LLHSYKRSFNGFVV+LTEEEAQKIS K+GVVSVFPN KKHLHTTRSWDFMG
Sbjct: 61  VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120

Query: 128 FTKNVPRVNQVESNIVVGVLDSGIWPESPSFSDVGFGLPPTKWKGTCQTSANFRCNRKII 187
           FTK+V RV QVESNIVVGVLDSGIWPESPSFSDVG+G PP KWKG CQTSANFRCNRKII
Sbjct: 121 FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180

Query: 188 GARAYRSDNFFPPEDIEGPRDSDGHGTHTASTVAGGLVDQASLFGLGLGTARGGV----- 247
           GARAYRSDN FPPEDI+ PRDSDGHGTHTASTVAGGLV+QASL+GL LGTARGGV     
Sbjct: 181 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240

Query: 248 -----CWSDGCHDADILAAFDDAIADGVDIISLSVGGNEPRFYFNDSIAIGAFHSMKHGI 307
                CWSDGC+DADILAAFDDAIADGVDIISLSVGGN+P++YFNDSIAIGAFHSMKHGI
Sbjct: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300

Query: 308 LTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSKLQLGNSKIYQGYTINTFDLLGK 367
           LTSNSAGNDGPDYFTIRNFSPWSLSVAAS+IDRKLVSK+QLGN  +YQGYTINTFDL GK
Sbjct: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360

Query: 368 QYPLIYAGDALNVTGGFTGLSSRKFSGSQSG----EGKILLCDSILAPSTFASFSDAVGV 427
           QYPLIYAG+A NV+GGFTG SSR  S +        GKILLCDSIL+PSTFASF+ AVGV
Sbjct: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420

Query: 428 VMNDAGVKDNSRSYPLPSSYLETADGDSIKSYMASNGVATVTIFKSDAVNDSSAPLIVSF 487
           VMNDAGVKDNSRSYPLPSSYL    G++IK+YM SN   T TIFKS+AVND+SAPLIVSF
Sbjct: 421 VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF 480

Query: 488 SSRGPNPETLDILKPDLTAPGVEILAAWSPNASVSSGVRDSRKTMYNIISGTSMSCPHAT 547
           SSRGPNPET DILKPDLTAPGVEILAAWSP ASVSSGV DSR T+YNIISGTSMSCPHAT
Sbjct: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540

Query: 548 AAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELNPEAEFAYGAGHIDPLKAINPGLVYDA 607
           AAAVYVKTFHP+WSPAAIKSALMTTAIPLN +LNP+AEFAYGAGHI+P+KA+NPGLVYDA
Sbjct: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600

Query: 608 NESDYVKFLCGQGYSTAMVRRLSGDGSVCTLANSGRVWDLNYPSFALSTAPSESINQFFR 667
            ESDYV+FLCGQGY+TAMVRRLSGD SVCT ANSGRVWDLNYPSFALS+ PSESINQFFR
Sbjct: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660

Query: 668 RTLTNVESKASTYRYKVLGAPQGLTITVDPPILSFNGIGQKESFTLTIRGTVRQSIVSAS 727
           RT+TNV SK +TYR KVLGAP+GLTI+V+PP LSFN IGQK+SFT+T+RG V Q IVSA+
Sbjct: 661 RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA 720

Query: 728 LVWTDGSHNVRSPITVYVVIK 733
           L+WTDG H+VRSPITVYVV K
Sbjct: 721 LLWTDGHHHVRSPITVYVVDK 741

BLAST of Tan0005919 vs. NCBI nr
Match: XP_022995146.1 (cucumisin-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 1218.8 bits (3152), Expect = 0.0e+00
Identity = 607/746 (81.37%), Postives = 663/746 (88.87%), Query Frame = 0

Query: 1   MTMTRNTMSSSLIFILVFLNLISNLLASSSDSDNDGRKIYIVYLGNKPEDSASTPSHHMR 60
           MTM  N  SSSLIF LV LNL+  LLASS DS NDGRK+YIVYLGNK ED+ASTPSHHMR
Sbjct: 5   MTMAGNNASSSLIFKLVILNLVCCLLASSFDSGNDGRKVYIVYLGNKREDTASTPSHHMR 64

Query: 61  MLEEVVGSKFAPKSLLHSYKRSFNGFVVRLTEEEAQKISGKKGVVSVFPNEKKHLHTTRS 120
           MLEE +GS FAP++LLHSYKRSFNGFVVRLTEEEAQKIS K GVVSVFPNEKKH HTTRS
Sbjct: 65  MLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRS 124

Query: 121 WDFMGFTKNVPRVNQVESNIVVGVLDSGIWPESPSFSDVGFGLPPTKWKGTCQTSANFRC 180
           WDFMGFT NVPRV QVES+IVVGVLD+GIWPESPSFSDVG+G PP KWKGTCQ S NFRC
Sbjct: 125 WDFMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNFRC 184

Query: 181 NRKIIGARAYRSDNFFPPEDIEGPRDSDGHGTHTASTVAGGLVDQASLFGLGLGTARGG- 240
           N+KIIGARAYRSDN FPPED + PRDS+GHGTHTASTVAGGLV QASLFGL LGTARGG 
Sbjct: 185 NKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGGV 244

Query: 241 ---------VCWSDGCHDADILAAFDDAIADGVDIISLSVGGNEPRFYFNDSIAIGAFHS 300
                    VCWSDGC DADILAAFDDAIADGVDIISLSVGGNEP+FYFNDSIAIGAFHS
Sbjct: 245 PSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFHS 304

Query: 301 MKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSKLQLGNSKIYQGYTINTF 360
           MK+GILTSNSAGNDGPD+ T+RNFSPWSLSVAAS+IDRKLVSK+QLGN+ I+QGYTINTF
Sbjct: 305 MKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGYTINTF 364

Query: 361 DLLGKQYPLIYAGDALNVTGGFTGLSSRKFSGSQSG----EGKILLCDSILAPSTFASFS 420
           DL GK YPLIYAG A N++GGF+G SSR  S +       +GKIL+CDSIL+PSTFAS S
Sbjct: 365 DLQGKHYPLIYAGHAPNISGGFSGSSSRYCSKNSVDRNLVKGKILVCDSILSPSTFASLS 424

Query: 421 DAVGVVMNDAGVKDNSRSYPLPSSYLETADGDSIKSYMASNGVATVTIFKSDAVNDSSAP 480
            AVGVVMN+AGVKDN+RSYPLPSSYL  A G+ +K+Y+ SN   T TIFKS+ VND++AP
Sbjct: 425 SAVGVVMNNAGVKDNARSYPLPSSYLTRAAGNHVKNYIGSNNFPTATIFKSNEVNDTAAP 484

Query: 481 LIVSFSSRGPNPETLDILKPDLTAPGVEILAAWSPNASVSSGVRDSRKTMYNIISGTSMS 540
           ++VSFSSRGPNPET DILKPDLTAPGVEILAAW+P A VSSG++DSR T+YNIISGTSMS
Sbjct: 485 IVVSFSSRGPNPETYDILKPDLTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMS 544

Query: 541 CPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELNPEAEFAYGAGHIDPLKAINPG 600
           CPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLN ELNP+AEFAYGAGHIDPLKA+NPG
Sbjct: 545 CPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVELNPQAEFAYGAGHIDPLKAMNPG 604

Query: 601 LVYDANESDYVKFLCGQGYSTAMVRRLSGDGSVCTLANSGRVWDLNYPSFALSTAPSESI 660
           L+YDANE+DYV FLCGQGYSTAMVRRL+GDGSVCT ANSGRVWDLNYPSFALST PSESI
Sbjct: 605 LIYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESI 664

Query: 661 NQFFRRTLTNVESKASTYRYKVLGAPQGLTITVDPPILSFNGIGQKESFTLTIRGTVRQS 720
           NQFF RTLTNV S+ASTY  K+LGAP+GLTITVDPP+LSFNGIG KESFTLTI GT+ QS
Sbjct: 665 NQFFTRTLTNVGSEASTYTSKILGAPEGLTITVDPPVLSFNGIGHKESFTLTIGGTISQS 724

Query: 721 IVSASLVWTDGSHNVRSPITVYVVIK 733
           IVSAS+VW+DGSHNVRSPIT+Y+V K
Sbjct: 725 IVSASVVWSDGSHNVRSPITIYIVNK 750

BLAST of Tan0005919 vs. NCBI nr
Match: XP_022941974.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 1216.4 bits (3146), Expect = 0.0e+00
Identity = 607/746 (81.37%), Postives = 665/746 (89.14%), Query Frame = 0

Query: 1   MTMTRNTMSSSLIFILVFLNLISNLLASSSDSDNDGRKIYIVYLGNKPEDSASTPSHHMR 60
           M M  N  SSSLIF LV LNL+  LLASS DS NDGRK+YIVYLGNK ED+ASTPSHH R
Sbjct: 39  MIMAGNNASSSLIFKLVVLNLVCCLLASSFDSGNDGRKVYIVYLGNKREDTASTPSHHTR 98

Query: 61  MLEEVVGSKFAPKSLLHSYKRSFNGFVVRLTEEEAQKISGKKGVVSVFPNEKKHLHTTRS 120
           MLEE +GS FAP++LLHSYKRSFNGFVVRLTEEEAQKIS K+GVVSVFPNEKKH HTTRS
Sbjct: 99  MLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKEGVVSVFPNEKKHPHTTRS 158

Query: 121 WDFMGFTKNVPRVNQVESNIVVGVLDSGIWPESPSFSDVGFGLPPTKWKGTCQTSANFRC 180
           WDFMGFT NVPRV QVES+IVVGVLD+GIWPESPSF+DVG+G PP KWKG CQTS NFRC
Sbjct: 159 WDFMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFNDVGYGPPPAKWKGACQTSTNFRC 218

Query: 181 NRKIIGARAYRSDNFFPPEDIEGPRDSDGHGTHTASTVAGGLVDQASLFGLGLGTARGGV 240
           N+KIIGARAYRSDN FPPED + PRDS+GHGTHTASTVAGGLV QASLFGL LGTARGGV
Sbjct: 219 NKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGGV 278

Query: 241 ----------CWSDGCHDADILAAFDDAIADGVDIISLSVGGNEPRFYFNDSIAIGAFHS 300
                     CWSDGC DADILAAFDDAIADGVDIISLSVGGNEP+FYFNDSIAIGAFHS
Sbjct: 279 PSARIAVYKICWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFHS 338

Query: 301 MKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSKLQLGNSKIYQGYTINTF 360
           MK+GILTSNSAGNDGPD+ TIRNFSPWSLSVAAS+IDRKLVSK+QLGN+ I+QG+TINTF
Sbjct: 339 MKNGILTSNSAGNDGPDFLTIRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINTF 398

Query: 361 DLLGKQYPLIYAGDALNVTGGFTGLSSRKFSGSQSG----EGKILLCDSILAPSTFASFS 420
           DL  KQ+PLIYAG+A N++GGFTG +SR  S +       +GKIL+CDSI++PSTFASFS
Sbjct: 399 DLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSIVSPSTFASFS 458

Query: 421 DAVGVVMNDAGVKDNSRSYPLPSSYLETADGDSIKSYMASNGVATVTIFKSDAVNDSSAP 480
            AVGVVMNDAGVKDN+RSYPLPSSYL T  G++IK+YM SN   T TIFKS+AVND+SAP
Sbjct: 459 SAVGVVMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSAP 518

Query: 481 LIVSFSSRGPNPETLDILKPDLTAPGVEILAAWSPNASVSSGVRDSRKTMYNIISGTSMS 540
           ++VSFSSRGPNPETLDILKPDLTAPGVEILAAW P+A VSSG+RDSR T+YNIISGTSMS
Sbjct: 519 IVVSFSSRGPNPETLDILKPDLTAPGVEILAAWPPDAPVSSGIRDSRSTLYNIISGTSMS 578

Query: 541 CPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELNPEAEFAYGAGHIDPLKAINPG 600
           CPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLN  LNP+AEFAYGAGHIDPLKA NPG
Sbjct: 579 CPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAEFAYGAGHIDPLKATNPG 638

Query: 601 LVYDANESDYVKFLCGQGYSTAMVRRLSGDGSVCTLANSGRVWDLNYPSFALSTAPSESI 660
           LVYDANE+DYV FLCGQGYSTAMVRRL+GDGSVCT ANSGRVWDLNYPSFALST PSESI
Sbjct: 639 LVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESI 698

Query: 661 NQFFRRTLTNVESKASTYRYKVLGAPQGLTITVDPPILSFNGIGQKESFTLTIRGTVRQS 720
           NQFF RTLTNV+++ASTY  K+LGAP+GLTITVDPP LSFNGIG K+SFTLTI GT+ Q+
Sbjct: 699 NQFFTRTLTNVDTEASTYTSKILGAPEGLTITVDPPALSFNGIGDKKSFTLTIDGTIIQT 758

Query: 721 IVSASLVWTDGSHNVRSPITVYVVIK 733
           IVSAS+VW+DGSHNVRSPIT+Y+V K
Sbjct: 759 IVSASVVWSDGSHNVRSPITIYIVNK 784

BLAST of Tan0005919 vs. NCBI nr
Match: XP_022941960.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 1214.9 bits (3142), Expect = 0.0e+00
Identity = 612/741 (82.59%), Postives = 667/741 (90.01%), Query Frame = 0

Query: 8   MSSSLIF-ILVFLNLISNLLASSSD-SDNDGRKIYIVYLGNKPEDSASTPSHHMRMLEEV 67
           MSSSLIF +++F++   +LLAS  D SDNDGRK+YIVYLGNKPEDSASTPSHHMRMLEEV
Sbjct: 1   MSSSLIFKLVIFISFSGSLLASGLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60

Query: 68  VGSKFAPKSLLHSYKRSFNGFVVRLTEEEAQKISGKKGVVSVFPNEKKHLHTTRSWDFMG 127
           VGS FAP +LLHSYKRSFNGFVV+LTEEEAQKIS K+GVVSVFPN KKHLHTTRSWDFMG
Sbjct: 61  VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120

Query: 128 FTKNVPRVNQVESNIVVGVLDSGIWPESPSFSDVGFGLPPTKWKGTCQTSANFRCNRKII 187
           FTKNV RV QVESNIVVGVLDSGIWPESPSFSDVG+G PP KWKG CQTSANFRCNRKII
Sbjct: 121 FTKNVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180

Query: 188 GARAYRSDNFFPPEDIEGPRDSDGHGTHTASTVAGGLVDQASLFGLGLGTARGGV----- 247
           GARAYRSDN FPPEDI+ PRDSDGHGTHTASTVAGGLV+QASL+GL LGTARGGV     
Sbjct: 181 GARAYRSDNSFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240

Query: 248 -----CWSDGCHDADILAAFDDAIADGVDIISLSVGGNEPRFYFNDSIAIGAFHSMKHGI 307
                CWSDGC+DADILAAFDDAIADGVDIISLSVGGN+P++YFNDSIAIGAFHSMKHGI
Sbjct: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300

Query: 308 LTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSKLQLGNSKIYQGYTINTFDLLGK 367
           LTSNSAGNDGPDYFTIRNFSPWSLSVAAS+IDRKLVSK+QLGN  +YQGYTINTFDL GK
Sbjct: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360

Query: 368 QYPLIYAGDALNVTGGFTGLSSRKFSGSQSG----EGKILLCDSILAPSTFASFSDAVGV 427
           QYPLIYAG+A NV+GGFTG SSR  S +        GKILLCDSIL+PSTFASF+ AVGV
Sbjct: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420

Query: 428 VMNDAGVKDNSRSYPLPSSYLETADGDSIKSYMASNGVATVTIFKSDAVNDSSAPLIVSF 487
           VMNDAGVKDN+RSYPLPSSYL    G++IK+YM S+   T TIFKS+AVND+SAPLIVSF
Sbjct: 421 VMNDAGVKDNARSYPLPSSYLGPVAGNNIKTYMGSSKFPTATIFKSNAVNDTSAPLIVSF 480

Query: 488 SSRGPNPETLDILKPDLTAPGVEILAAWSPNASVSSGVRDSRKTMYNIISGTSMSCPHAT 547
           SSRGPNPET DILKPDLTAPGVEILAAWSP ASVSSGV DSR T+YNIISGTSMSCPHAT
Sbjct: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540

Query: 548 AAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELNPEAEFAYGAGHIDPLKAINPGLVYDA 607
           AAAVYVKTFHP+WSPAAIKSALMTTAIPLN +LNP+AEFAYGAGHI+P+KA+NPGLVYDA
Sbjct: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600

Query: 608 NESDYVKFLCGQGYSTAMVRRLSGDGSVCTLANSGRVWDLNYPSFALSTAPSESINQFFR 667
            ESDYV+FLCGQGY+TAMVRRLSGD SVCT ANSGRVWDLNYPSFALS+ PSESINQFFR
Sbjct: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTHANSGRVWDLNYPSFALSSTPSESINQFFR 660

Query: 668 RTLTNVESKASTYRYKVLGAPQGLTITVDPPILSFNGIGQKESFTLTIRGTVRQSIVSAS 727
           RT+TNV SK +TYR KVLG P+GLTI+V+PP+LSFN IGQK+SFT+T+RG V Q IVSA+
Sbjct: 661 RTVTNVGSKVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGGVNQPIVSAA 720

Query: 728 LVWTDGSHNVRSPITVYVVIK 733
           L+WTDG H+VRSPITVYVV K
Sbjct: 721 LLWTDGHHHVRSPITVYVVDK 741

BLAST of Tan0005919 vs. ExPASy TrEMBL
Match: A0A6J1IQ27 (cucumisin-like OS=Cucurbita maxima OX=3661 GN=LOC111478984 PE=3 SV=1)

HSP 1 Score: 1218.8 bits (3152), Expect = 0.0e+00
Identity = 615/741 (83.00%), Postives = 668/741 (90.15%), Query Frame = 0

Query: 8   MSSSLIF-ILVFLNLISNLLASSSD-SDNDGRKIYIVYLGNKPEDSASTPSHHMRMLEEV 67
           MSSSLIF +++F++   +LLASS D SDNDGRK+YIVYLGNKPEDSASTPSHHMRMLEEV
Sbjct: 1   MSSSLIFKLVIFISFSGSLLASSLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60

Query: 68  VGSKFAPKSLLHSYKRSFNGFVVRLTEEEAQKISGKKGVVSVFPNEKKHLHTTRSWDFMG 127
           VGS FAP +LLHSYKRSFNGFVV+LTEEEAQKIS K+GVVSVFPN KKHLHTTRSWDFMG
Sbjct: 61  VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120

Query: 128 FTKNVPRVNQVESNIVVGVLDSGIWPESPSFSDVGFGLPPTKWKGTCQTSANFRCNRKII 187
           FTK+V RV QVESNIVVGVLDSGIWPESPSFSDVG+G PP KWKG CQTSANFRCNRKII
Sbjct: 121 FTKSVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180

Query: 188 GARAYRSDNFFPPEDIEGPRDSDGHGTHTASTVAGGLVDQASLFGLGLGTARGGV----- 247
           GARAYRSDN FPPEDI+ PRDSDGHGTHTASTVAGGLV+QASL+GL LGTARGGV     
Sbjct: 181 GARAYRSDNLFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240

Query: 248 -----CWSDGCHDADILAAFDDAIADGVDIISLSVGGNEPRFYFNDSIAIGAFHSMKHGI 307
                CWSDGC+DADILAAFDDAIADGVDIISLSVGGN+P++YFNDSIAIGAFHSMKHGI
Sbjct: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300

Query: 308 LTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSKLQLGNSKIYQGYTINTFDLLGK 367
           LTSNSAGNDGPDYFTIRNFSPWSLSVAAS+IDRKLVSK+QLGN  +YQGYTINTFDL GK
Sbjct: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360

Query: 368 QYPLIYAGDALNVTGGFTGLSSRKFSGSQSG----EGKILLCDSILAPSTFASFSDAVGV 427
           QYPLIYAG+A NV+GGFTG SSR  S +        GKILLCDSIL+PSTFASF+ AVGV
Sbjct: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420

Query: 428 VMNDAGVKDNSRSYPLPSSYLETADGDSIKSYMASNGVATVTIFKSDAVNDSSAPLIVSF 487
           VMNDAGVKDNSRSYPLPSSYL    G++IK+YM SN   T TIFKS+AVND+SAPLIVSF
Sbjct: 421 VMNDAGVKDNSRSYPLPSSYLGPVAGNNIKTYMGSNKFPTATIFKSNAVNDTSAPLIVSF 480

Query: 488 SSRGPNPETLDILKPDLTAPGVEILAAWSPNASVSSGVRDSRKTMYNIISGTSMSCPHAT 547
           SSRGPNPET DILKPDLTAPGVEILAAWSP ASVSSGV DSR T+YNIISGTSMSCPHAT
Sbjct: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540

Query: 548 AAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELNPEAEFAYGAGHIDPLKAINPGLVYDA 607
           AAAVYVKTFHP+WSPAAIKSALMTTAIPLN +LNP+AEFAYGAGHI+P+KA+NPGLVYDA
Sbjct: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600

Query: 608 NESDYVKFLCGQGYSTAMVRRLSGDGSVCTLANSGRVWDLNYPSFALSTAPSESINQFFR 667
            ESDYV+FLCGQGY+TAMVRRLSGD SVCT ANSGRVWDLNYPSFALS+ PSESINQFFR
Sbjct: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTRANSGRVWDLNYPSFALSSTPSESINQFFR 660

Query: 668 RTLTNVESKASTYRYKVLGAPQGLTITVDPPILSFNGIGQKESFTLTIRGTVRQSIVSAS 727
           RT+TNV SK +TYR KVLGAP+GLTI+V+PP LSFN IGQK+SFT+T+RG V Q IVSA+
Sbjct: 661 RTVTNVGSKVATYRAKVLGAPRGLTISVNPPALSFNAIGQKKSFTVTVRGGVNQPIVSAA 720

Query: 728 LVWTDGSHNVRSPITVYVVIK 733
           L+WTDG H+VRSPITVYVV K
Sbjct: 721 LLWTDGHHHVRSPITVYVVDK 741

BLAST of Tan0005919 vs. ExPASy TrEMBL
Match: A0A6J1K168 (cucumisin-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111490766 PE=3 SV=1)

HSP 1 Score: 1218.8 bits (3152), Expect = 0.0e+00
Identity = 607/746 (81.37%), Postives = 663/746 (88.87%), Query Frame = 0

Query: 1   MTMTRNTMSSSLIFILVFLNLISNLLASSSDSDNDGRKIYIVYLGNKPEDSASTPSHHMR 60
           MTM  N  SSSLIF LV LNL+  LLASS DS NDGRK+YIVYLGNK ED+ASTPSHHMR
Sbjct: 5   MTMAGNNASSSLIFKLVILNLVCCLLASSFDSGNDGRKVYIVYLGNKREDTASTPSHHMR 64

Query: 61  MLEEVVGSKFAPKSLLHSYKRSFNGFVVRLTEEEAQKISGKKGVVSVFPNEKKHLHTTRS 120
           MLEE +GS FAP++LLHSYKRSFNGFVVRLTEEEAQKIS K GVVSVFPNEKKH HTTRS
Sbjct: 65  MLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKDGVVSVFPNEKKHPHTTRS 124

Query: 121 WDFMGFTKNVPRVNQVESNIVVGVLDSGIWPESPSFSDVGFGLPPTKWKGTCQTSANFRC 180
           WDFMGFT NVPRV QVES+IVVGVLD+GIWPESPSFSDVG+G PP KWKGTCQ S NFRC
Sbjct: 125 WDFMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFSDVGYGPPPAKWKGTCQASTNFRC 184

Query: 181 NRKIIGARAYRSDNFFPPEDIEGPRDSDGHGTHTASTVAGGLVDQASLFGLGLGTARGG- 240
           N+KIIGARAYRSDN FPPED + PRDS+GHGTHTASTVAGGLV QASLFGL LGTARGG 
Sbjct: 185 NKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGGV 244

Query: 241 ---------VCWSDGCHDADILAAFDDAIADGVDIISLSVGGNEPRFYFNDSIAIGAFHS 300
                    VCWSDGC DADILAAFDDAIADGVDIISLSVGGNEP+FYFNDSIAIGAFHS
Sbjct: 245 PSARIAVYKVCWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFHS 304

Query: 301 MKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSKLQLGNSKIYQGYTINTF 360
           MK+GILTSNSAGNDGPD+ T+RNFSPWSLSVAAS+IDRKLVSK+QLGN+ I+QGYTINTF
Sbjct: 305 MKNGILTSNSAGNDGPDFLTVRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGYTINTF 364

Query: 361 DLLGKQYPLIYAGDALNVTGGFTGLSSRKFSGSQSG----EGKILLCDSILAPSTFASFS 420
           DL GK YPLIYAG A N++GGF+G SSR  S +       +GKIL+CDSIL+PSTFAS S
Sbjct: 365 DLQGKHYPLIYAGHAPNISGGFSGSSSRYCSKNSVDRNLVKGKILVCDSILSPSTFASLS 424

Query: 421 DAVGVVMNDAGVKDNSRSYPLPSSYLETADGDSIKSYMASNGVATVTIFKSDAVNDSSAP 480
            AVGVVMN+AGVKDN+RSYPLPSSYL  A G+ +K+Y+ SN   T TIFKS+ VND++AP
Sbjct: 425 SAVGVVMNNAGVKDNARSYPLPSSYLTRAAGNHVKNYIGSNNFPTATIFKSNEVNDTAAP 484

Query: 481 LIVSFSSRGPNPETLDILKPDLTAPGVEILAAWSPNASVSSGVRDSRKTMYNIISGTSMS 540
           ++VSFSSRGPNPET DILKPDLTAPGVEILAAW+P A VSSG++DSR T+YNIISGTSMS
Sbjct: 485 IVVSFSSRGPNPETYDILKPDLTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMS 544

Query: 541 CPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELNPEAEFAYGAGHIDPLKAINPG 600
           CPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLN ELNP+AEFAYGAGHIDPLKA+NPG
Sbjct: 545 CPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVELNPQAEFAYGAGHIDPLKAMNPG 604

Query: 601 LVYDANESDYVKFLCGQGYSTAMVRRLSGDGSVCTLANSGRVWDLNYPSFALSTAPSESI 660
           L+YDANE+DYV FLCGQGYSTAMVRRL+GDGSVCT ANSGRVWDLNYPSFALST PSESI
Sbjct: 605 LIYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESI 664

Query: 661 NQFFRRTLTNVESKASTYRYKVLGAPQGLTITVDPPILSFNGIGQKESFTLTIRGTVRQS 720
           NQFF RTLTNV S+ASTY  K+LGAP+GLTITVDPP+LSFNGIG KESFTLTI GT+ QS
Sbjct: 665 NQFFTRTLTNVGSEASTYTSKILGAPEGLTITVDPPVLSFNGIGHKESFTLTIGGTISQS 724

Query: 721 IVSASLVWTDGSHNVRSPITVYVVIK 733
           IVSAS+VW+DGSHNVRSPIT+Y+V K
Sbjct: 725 IVSASVVWSDGSHNVRSPITIYIVNK 750

BLAST of Tan0005919 vs. ExPASy TrEMBL
Match: A0A6J1FQ02 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111447181 PE=3 SV=1)

HSP 1 Score: 1216.4 bits (3146), Expect = 0.0e+00
Identity = 607/746 (81.37%), Postives = 665/746 (89.14%), Query Frame = 0

Query: 1   MTMTRNTMSSSLIFILVFLNLISNLLASSSDSDNDGRKIYIVYLGNKPEDSASTPSHHMR 60
           M M  N  SSSLIF LV LNL+  LLASS DS NDGRK+YIVYLGNK ED+ASTPSHH R
Sbjct: 39  MIMAGNNASSSLIFKLVVLNLVCCLLASSFDSGNDGRKVYIVYLGNKREDTASTPSHHTR 98

Query: 61  MLEEVVGSKFAPKSLLHSYKRSFNGFVVRLTEEEAQKISGKKGVVSVFPNEKKHLHTTRS 120
           MLEE +GS FAP++LLHSYKRSFNGFVVRLTEEEAQKIS K+GVVSVFPNEKKH HTTRS
Sbjct: 99  MLEETIGSTFAPEALLHSYKRSFNGFVVRLTEEEAQKISAKEGVVSVFPNEKKHPHTTRS 158

Query: 121 WDFMGFTKNVPRVNQVESNIVVGVLDSGIWPESPSFSDVGFGLPPTKWKGTCQTSANFRC 180
           WDFMGFT NVPRV QVES+IVVGVLD+GIWPESPSF+DVG+G PP KWKG CQTS NFRC
Sbjct: 159 WDFMGFTTNVPRVKQVESDIVVGVLDTGIWPESPSFNDVGYGPPPAKWKGACQTSTNFRC 218

Query: 181 NRKIIGARAYRSDNFFPPEDIEGPRDSDGHGTHTASTVAGGLVDQASLFGLGLGTARGGV 240
           N+KIIGARAYRSDN FPPED + PRDS+GHGTHTASTVAGGLV QASLFGL LGTARGGV
Sbjct: 219 NKKIIGARAYRSDNNFPPEDFKSPRDSEGHGTHTASTVAGGLVSQASLFGLALGTARGGV 278

Query: 241 ----------CWSDGCHDADILAAFDDAIADGVDIISLSVGGNEPRFYFNDSIAIGAFHS 300
                     CWSDGC DADILAAFDDAIADGVDIISLSVGGNEP+FYFNDSIAIGAFHS
Sbjct: 279 PSARIAVYKICWSDGCEDADILAAFDDAIADGVDIISLSVGGNEPKFYFNDSIAIGAFHS 338

Query: 301 MKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSKLQLGNSKIYQGYTINTF 360
           MK+GILTSNSAGNDGPD+ TIRNFSPWSLSVAAS+IDRKLVSK+QLGN+ I+QG+TINTF
Sbjct: 339 MKNGILTSNSAGNDGPDFLTIRNFSPWSLSVAASSIDRKLVSKVQLGNNNIFQGFTINTF 398

Query: 361 DLLGKQYPLIYAGDALNVTGGFTGLSSRKFSGSQSG----EGKILLCDSILAPSTFASFS 420
           DL  KQ+PLIYAG+A N++GGFTG +SR  S +       +GKIL+CDSI++PSTFASFS
Sbjct: 399 DLQQKQFPLIYAGNAPNISGGFTGATSRFCSRNSVDRNLVKGKILVCDSIVSPSTFASFS 458

Query: 421 DAVGVVMNDAGVKDNSRSYPLPSSYLETADGDSIKSYMASNGVATVTIFKSDAVNDSSAP 480
            AVGVVMNDAGVKDN+RSYPLPSSYL T  G++IK+YM SN   T TIFKS+AVND+SAP
Sbjct: 459 SAVGVVMNDAGVKDNARSYPLPSSYLGTVAGNNIKTYMGSNNFPTATIFKSNAVNDTSAP 518

Query: 481 LIVSFSSRGPNPETLDILKPDLTAPGVEILAAWSPNASVSSGVRDSRKTMYNIISGTSMS 540
           ++VSFSSRGPNPETLDILKPDLTAPGVEILAAW P+A VSSG+RDSR T+YNIISGTSMS
Sbjct: 519 IVVSFSSRGPNPETLDILKPDLTAPGVEILAAWPPDAPVSSGIRDSRSTLYNIISGTSMS 578

Query: 541 CPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELNPEAEFAYGAGHIDPLKAINPG 600
           CPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLN  LNP+AEFAYGAGHIDPLKA NPG
Sbjct: 579 CPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVGLNPQAEFAYGAGHIDPLKATNPG 638

Query: 601 LVYDANESDYVKFLCGQGYSTAMVRRLSGDGSVCTLANSGRVWDLNYPSFALSTAPSESI 660
           LVYDANE+DYV FLCGQGYSTAMVRRL+GDGSVCT ANSGRVWDLNYPSFALST PSESI
Sbjct: 639 LVYDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESI 698

Query: 661 NQFFRRTLTNVESKASTYRYKVLGAPQGLTITVDPPILSFNGIGQKESFTLTIRGTVRQS 720
           NQFF RTLTNV+++ASTY  K+LGAP+GLTITVDPP LSFNGIG K+SFTLTI GT+ Q+
Sbjct: 699 NQFFTRTLTNVDTEASTYTSKILGAPEGLTITVDPPALSFNGIGDKKSFTLTIDGTIIQT 758

Query: 721 IVSASLVWTDGSHNVRSPITVYVVIK 733
           IVSAS+VW+DGSHNVRSPIT+Y+V K
Sbjct: 759 IVSASVVWSDGSHNVRSPITIYIVNK 784

BLAST of Tan0005919 vs. ExPASy TrEMBL
Match: A0A6J1FV97 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111447169 PE=3 SV=1)

HSP 1 Score: 1214.9 bits (3142), Expect = 0.0e+00
Identity = 612/741 (82.59%), Postives = 667/741 (90.01%), Query Frame = 0

Query: 8   MSSSLIF-ILVFLNLISNLLASSSD-SDNDGRKIYIVYLGNKPEDSASTPSHHMRMLEEV 67
           MSSSLIF +++F++   +LLAS  D SDNDGRK+YIVYLGNKPEDSASTPSHHMRMLEEV
Sbjct: 1   MSSSLIFKLVIFISFSGSLLASGLDSSDNDGRKVYIVYLGNKPEDSASTPSHHMRMLEEV 60

Query: 68  VGSKFAPKSLLHSYKRSFNGFVVRLTEEEAQKISGKKGVVSVFPNEKKHLHTTRSWDFMG 127
           VGS FAP +LLHSYKRSFNGFVV+LTEEEAQKIS K+GVVSVFPN KKHLHTTRSWDFMG
Sbjct: 61  VGSTFAPDALLHSYKRSFNGFVVKLTEEEAQKISAKEGVVSVFPNGKKHLHTTRSWDFMG 120

Query: 128 FTKNVPRVNQVESNIVVGVLDSGIWPESPSFSDVGFGLPPTKWKGTCQTSANFRCNRKII 187
           FTKNV RV QVESNIVVGVLDSGIWPESPSFSDVG+G PP KWKG CQTSANFRCNRKII
Sbjct: 121 FTKNVRRVKQVESNIVVGVLDSGIWPESPSFSDVGYGPPPAKWKGACQTSANFRCNRKII 180

Query: 188 GARAYRSDNFFPPEDIEGPRDSDGHGTHTASTVAGGLVDQASLFGLGLGTARGGV----- 247
           GARAYRSDN FPPEDI+ PRDSDGHGTHTASTVAGGLV+QASL+GL LGTARGGV     
Sbjct: 181 GARAYRSDNSFPPEDIKSPRDSDGHGTHTASTVAGGLVNQASLYGLALGTARGGVPSARI 240

Query: 248 -----CWSDGCHDADILAAFDDAIADGVDIISLSVGGNEPRFYFNDSIAIGAFHSMKHGI 307
                CWSDGC+DADILAAFDDAIADGVDIISLSVGGN+P++YFNDSIAIGAFHSMKHGI
Sbjct: 241 AVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGNKPKYYFNDSIAIGAFHSMKHGI 300

Query: 308 LTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSKLQLGNSKIYQGYTINTFDLLGK 367
           LTSNSAGNDGPDYFTIRNFSPWSLSVAAS+IDRKLVSK+QLGN  +YQGYTINTFDL GK
Sbjct: 301 LTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSKVQLGNKNVYQGYTINTFDLKGK 360

Query: 368 QYPLIYAGDALNVTGGFTGLSSRKFSGSQSG----EGKILLCDSILAPSTFASFSDAVGV 427
           QYPLIYAG+A NV+GGFTG SSR  S +        GKILLCDSIL+PSTFASF+ AVGV
Sbjct: 361 QYPLIYAGNAPNVSGGFTGSSSRFCSRNSVDRNLVRGKILLCDSILSPSTFASFNGAVGV 420

Query: 428 VMNDAGVKDNSRSYPLPSSYLETADGDSIKSYMASNGVATVTIFKSDAVNDSSAPLIVSF 487
           VMNDAGVKDN+RSYPLPSSYL    G++IK+YM S+   T TIFKS+AVND+SAPLIVSF
Sbjct: 421 VMNDAGVKDNARSYPLPSSYLGPVAGNNIKTYMGSSKFPTATIFKSNAVNDTSAPLIVSF 480

Query: 488 SSRGPNPETLDILKPDLTAPGVEILAAWSPNASVSSGVRDSRKTMYNIISGTSMSCPHAT 547
           SSRGPNPET DILKPDLTAPGVEILAAWSP ASVSSGV DSR T+YNIISGTSMSCPHAT
Sbjct: 481 SSRGPNPETYDILKPDLTAPGVEILAAWSPIASVSSGVSDSRTTLYNIISGTSMSCPHAT 540

Query: 548 AAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELNPEAEFAYGAGHIDPLKAINPGLVYDA 607
           AAAVYVKTFHP+WSPAAIKSALMTTAIPLN +LNP+AEFAYGAGHI+P+KA+NPGLVYDA
Sbjct: 541 AAAVYVKTFHPSWSPAAIKSALMTTAIPLNPKLNPQAEFAYGAGHINPVKAVNPGLVYDA 600

Query: 608 NESDYVKFLCGQGYSTAMVRRLSGDGSVCTLANSGRVWDLNYPSFALSTAPSESINQFFR 667
            ESDYV+FLCGQGY+TAMVRRLSGD SVCT ANSGRVWDLNYPSFALS+ PSESINQFFR
Sbjct: 601 QESDYVRFLCGQGYTTAMVRRLSGDHSVCTHANSGRVWDLNYPSFALSSTPSESINQFFR 660

Query: 668 RTLTNVESKASTYRYKVLGAPQGLTITVDPPILSFNGIGQKESFTLTIRGTVRQSIVSAS 727
           RT+TNV SK +TYR KVLG P+GLTI+V+PP+LSFN IGQK+SFT+T+RG V Q IVSA+
Sbjct: 661 RTVTNVGSKVATYRAKVLGVPRGLTISVNPPVLSFNAIGQKKSFTVTVRGGVNQPIVSAA 720

Query: 728 LVWTDGSHNVRSPITVYVVIK 733
           L+WTDG H+VRSPITVYVV K
Sbjct: 721 LLWTDGHHHVRSPITVYVVDK 741

BLAST of Tan0005919 vs. ExPASy TrEMBL
Match: A0A6J1K719 (cucumisin-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490766 PE=3 SV=1)

HSP 1 Score: 1214.5 bits (3141), Expect = 0.0e+00
Identity = 604/744 (81.18%), Postives = 659/744 (88.58%), Query Frame = 0

Query: 3   MTRNTMSSSLIFILVFLNLISNLLASSSDSDNDGRKIYIVYLGNKPEDSASTPSHHMRML 62
           +TR+ M SS+IF LVFL     LLASS DSDNDGRK+YIVYLGNKP+D ASTPSHHMR+L
Sbjct: 8   LTRSIMGSSVIFKLVFLGFFGCLLASSLDSDNDGRKVYIVYLGNKPDDMASTPSHHMRLL 67

Query: 63  EEVVGSKFAPKSLLHSYKRSFNGFVVRLTEEEAQKISGKKGVVSVFPNEKKHLHTTRSWD 122
           EEVVGS F+P +LLHSYKRSFNGF VRLTEEEAQKI  K GVVSVFPN KKHLHTTRSWD
Sbjct: 68  EEVVGSTFSPDALLHSYKRSFNGFAVRLTEEEAQKIRAKDGVVSVFPNGKKHLHTTRSWD 127

Query: 123 FMGFTKNVPRVNQVESNIVVGVLDSGIWPESPSFSDVGFGLPPTKWKGTCQTSANFRCNR 182
           FMGFT NVPRVNQVES+IVVGVLDSGIWPESPSFSDVG+G  P KWKG CQ S NFRCN+
Sbjct: 128 FMGFTTNVPRVNQVESDIVVGVLDSGIWPESPSFSDVGYGPLPAKWKGACQASNNFRCNK 187

Query: 183 KIIGARAYRSDNFFPPEDIEGPRDSDGHGTHTASTVAGGLVDQASLFGLGLGTARGGV-- 242
           KIIGARAYRSDN FPPED E PRDSDGHGTHTASTVAGGLV QASL+GL LGTARGGV  
Sbjct: 188 KIIGARAYRSDNNFPPEDFESPRDSDGHGTHTASTVAGGLVSQASLYGLALGTARGGVPS 247

Query: 243 --------CWSDGCHDADILAAFDDAIADGVDIISLSVGGNEPRFYFNDSIAIGAFHSMK 302
                   CWSDGC+DADILAAFDDAIADGVDIISLSVGG+EP++YFNDSIAIGAFHSMK
Sbjct: 248 ARIAVYKICWSDGCYDADILAAFDDAIADGVDIISLSVGGSEPKYYFNDSIAIGAFHSMK 307

Query: 303 HGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSKLQLGNSKIYQGYTINTFDL 362
           HGILTSNSAGNDGPDYFTIRNFSPWSLSVAAS+IDRKLVS +QLGN  I+QGYTINTFDL
Sbjct: 308 HGILTSNSAGNDGPDYFTIRNFSPWSLSVAASSIDRKLVSDVQLGNKNIFQGYTINTFDL 367

Query: 363 LGKQYPLIYAGDALNVTGGFTGLSSRKFSGSQSG----EGKILLCDSILAPSTFASFSDA 422
            GK YPLIYAG A N++GGF+G SSR  S +       +GKIL+CDSIL+PSTFAS S A
Sbjct: 368 QGKHYPLIYAGHAPNISGGFSGSSSRYCSKNSVDRNLVKGKILVCDSILSPSTFASLSSA 427

Query: 423 VGVVMNDAGVKDNSRSYPLPSSYLETADGDSIKSYMASNGVATVTIFKSDAVNDSSAPLI 482
           VGVVMN+AGVKDN+RSYPLPSSYL  A G+ +K+Y+ SN   T TIFKS+ VND++AP++
Sbjct: 428 VGVVMNNAGVKDNARSYPLPSSYLTRAAGNHVKNYIGSNNFPTATIFKSNEVNDTAAPIV 487

Query: 483 VSFSSRGPNPETLDILKPDLTAPGVEILAAWSPNASVSSGVRDSRKTMYNIISGTSMSCP 542
           VSFSSRGPNPET DILKPDLTAPGVEILAAW+P A VSSG++DSR T+YNIISGTSMSCP
Sbjct: 488 VSFSSRGPNPETYDILKPDLTAPGVEILAAWAPIAPVSSGIKDSRTTLYNIISGTSMSCP 547

Query: 543 HATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELNPEAEFAYGAGHIDPLKAINPGLV 602
           HATAAAVYVKTFHPTWSPAAIKSALMTTAIPLN ELNP+AEFAYGAGHIDPLKA+NPGL+
Sbjct: 548 HATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNVELNPQAEFAYGAGHIDPLKAMNPGLI 607

Query: 603 YDANESDYVKFLCGQGYSTAMVRRLSGDGSVCTLANSGRVWDLNYPSFALSTAPSESINQ 662
           YDANE+DYV FLCGQGYSTAMVRRL+GDGSVCT ANSGRVWDLNYPSFALST PSESINQ
Sbjct: 608 YDANETDYVNFLCGQGYSTAMVRRLTGDGSVCTAANSGRVWDLNYPSFALSTTPSESINQ 667

Query: 663 FFRRTLTNVESKASTYRYKVLGAPQGLTITVDPPILSFNGIGQKESFTLTIRGTVRQSIV 722
           FF RTLTNV S+ASTY  K+LGAP+GLTITVDPP+LSFNGIG KESFTLTI GT+ QSIV
Sbjct: 668 FFTRTLTNVGSEASTYTSKILGAPEGLTITVDPPVLSFNGIGHKESFTLTIGGTISQSIV 727

Query: 723 SASLVWTDGSHNVRSPITVYVVIK 733
           SAS+VW+DGSHNVRSPIT+Y+V K
Sbjct: 728 SASVVWSDGSHNVRSPITIYIVNK 751

BLAST of Tan0005919 vs. TAIR 10
Match: AT5G59190.1 (subtilase family protein )

HSP 1 Score: 612.1 bits (1577), Expect = 6.0e-175
Identity = 325/702 (46.30%), Postives = 448/702 (63.82%), Query Frame = 0

Query: 44  LGNKPEDSASTPSHHMRMLEEVVGSKFAPKSLLHSYKRSFNGFVVRLTEEEAQKISGKKG 103
           +G  PE   S PSHH+ +L+++VG+  A   L+ SYKRSFNGF   L++ E+QK+   K 
Sbjct: 1   MGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKLQNMKE 60

Query: 104 VVSVFPNEKKHLHTTRSWDFMGFTKNVPRVNQVESNIVVGVLDSGIWPESPSFSDVGFGL 163
           VVSVFP++   L TTRSWDF+GF +   R +  ES+++VGV+DSGIWPES SF D GFG 
Sbjct: 61  VVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDDEGFGP 120

Query: 164 PPTKWKGTCQTSANFRCNRKIIGARAYRSDNFFPPEDIEGPRDSDGHGTHTASTVAGGLV 223
           PP KWKG+C+    F CN K+IGAR Y        +  +  RD +GHGTHTAST AG  V
Sbjct: 121 PPKKWKGSCKGGLKFACNNKLIGARFYN-------KFADSARDEEGHGTHTASTAAGNAV 180

Query: 224 DQASLFGLGLGTARGG----------VCWSDGCHDADILAAFDDAIADGVDIISLSVGGN 283
             AS +GL  GTARGG          VC+ + C+D DILAAFDDAIADGVD+IS+S+  +
Sbjct: 181 QAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISISISAD 240

Query: 284 EPRFYFNDSIAIGAFHSMKHGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSK 343
                 N S+AIG+FH+M  GI+T+ SAGN+GPD  ++ N SPW ++VAAS  DR+ + +
Sbjct: 241 YVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDRQFIDR 300

Query: 344 LQLGNSKIYQGYTINTFDLLGKQYPLIYAGD-ALNVTGGFTGLSSRKFSGSQSGEGKILL 403
           + LGN K   G ++NTF+L G ++P++Y  + + N +    G  S     S+  +GKI+L
Sbjct: 301 VVLGNGKALTGISVNTFNLNGTKFPIVYGQNVSRNCSQAQAGYCSSGCVDSELVKGKIVL 360

Query: 404 CDSILAPSTFASFSDAVGVVMNDAGVKDNSRSYPLPSSYLETADGDSIKSYMASNGVATV 463
           CD  L     A  + A+GV++ +  + D++   P P+S L   D  SIKSY+ S      
Sbjct: 361 CDDFLGYRE-AYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESAEPPQA 420

Query: 464 TIFKSDAVNDSSAPLIVSFSSRGPNPETLDILKPDLTAPGVEILAAWSPNASVSS--GVR 523
            I +++ + D  AP + SFSSRGP+    ++LKPD++APG+EILAA+SP AS SS     
Sbjct: 421 EILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSSFLNPE 480

Query: 524 DSRKTMYNIISGTSMSCPHATAAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELNPEAEF 583
           D R   Y+++SGTSM+CPH    A YVK+FHP WSP+AIKSA+MTTA P+N + NPE EF
Sbjct: 481 DKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKNPEQEF 540

Query: 584 AYGAGHIDPLKAINPGLVYDANESDYVKFLCGQGYSTAMVRRLSGDGSVCTLANSGRVWD 643
           AYG+G I+P KA +PGLVY+    DY+K LC +G+ +  +   SG    C  +    V D
Sbjct: 541 AYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTC--SERTEVKD 600

Query: 644 LNYPSFALSTAPSESINQFFRRTLTNVESKASTYRYKVLGAPQGLTITVDPPILSFNGIG 703
           LNYP+     +  +  N  F+RT+TNV    STY+  V+     L I+++P IL F  + 
Sbjct: 601 LNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILRFGFLE 660

Query: 704 QKESFTLTIRGTVRQ--SIVSASLVWTDGSHNVRSPITVYVV 731
           +K+SF +TI G   +  S VS+S+VW+DGSH+VRSPI  Y +
Sbjct: 661 EKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSI 691

BLAST of Tan0005919 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 608.2 bits (1567), Expect = 8.6e-174
Identity = 344/741 (46.42%), Postives = 458/741 (61.81%), Query Frame = 0

Query: 9   SSSLIFILVFLNLISNLLASSSDSDNDGRKIYIVYLGNKPEDSASTP-SHHMRMLEEVVG 68
           SSSL+  L+ L L      SS  +  D +++YIVY+G+    +  TP S HM +L+EV G
Sbjct: 7   SSSLLSCLLVLFL------SSVSAVTDDKQVYIVYMGSLSSRADYTPTSDHMNILQEVTG 66

Query: 69  SKFAPKSLLHSYKRSFNGFVVRLTEEEAQKISGKKGVVSVFPNEKKHLHTTRSWDFMGFT 128
                  L+ SYKRSFNGF  RLTE E ++++   GVVSVFPN+K  L TT SWDFMG  
Sbjct: 67  ESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMGLK 126

Query: 129 KNV--PRVNQVESNIVVGVLDSGIWPESPSFSDVGFGLPPTKWKGTCQTSANFRCNRKII 188
           + +   R   VES+ ++GV+DSGI PES SFSD GFG PP KWKG C    NF CN K+I
Sbjct: 127 EGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNKLI 186

Query: 189 GARAYRSDNFFPPEDIEGPRDSDGHGTHTASTVAGGLVDQASLFGLGLGTARGG------ 248
           GAR Y S         EG RD DGHGTHTAST AG  V  AS FG+G GT RGG      
Sbjct: 187 GARDYTS---------EGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPASRV 246

Query: 249 ----VCWSDGCHDADILAAFDDAIADGVDIISLSVGGNEPRFYFNDSIAIGAFHSMKHGI 308
               VC   GC    +L+AFDDAIADGVD+I++S+G      + ND IAIGAFH+M  G+
Sbjct: 247 AAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAKGV 306

Query: 309 LTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSKLQLGNSKIYQGYTINTFDLLGK 368
           LT NSAGN GP   ++   +PW L+VAAST +R  V+K+ LGN K   G ++N +++ GK
Sbjct: 307 LTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMKGK 366

Query: 369 QYPLIYAGDALN--VTGGFTGLSSRKFSGSQSGEGKILLCDSILAPSTFASFSDAVGVVM 428
            YPL+Y   A +        GL           +GKIL+C          S   AVG++ 
Sbjct: 367 DYPLVYGKSAASSACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESVG-AVGLIY 426

Query: 429 NDAGVKDNSRSYPLPSSYLETADGDSIKSYMASNGVATVTIFKSDAVNDSSAPLIVSFSS 488
                 D +  +PLP++ L T D +S+ SY+ S       + K++A+ + ++P+I SFSS
Sbjct: 427 R-TPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFSS 486

Query: 489 RGPNPETLDILKPDLTAPGVEILAAWSPNASVSSGVRDSRKTMYNIISGTSMSCPHATAA 548
           RGPN   +DILKPD+TAPGVEILAA+SP    S    D+R   Y+++SGTSMSCPH    
Sbjct: 487 RGPNTIAVDILKPDITAPGVEILAAYSPAGEPSQD--DTRHVKYSVLSGTSMSCPHVAGV 546

Query: 549 AVYVKTFHPTWSPAAIKSALMTTAIPLNAELN--PEAEFAYGAGHIDPLKAINPGLVYDA 608
           A YVKTF+P WSP+ I+SA+MTTA P+NA        EFAYG+GH+DP+ A NPGLVY+ 
Sbjct: 547 AAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYEL 606

Query: 609 NESDYVKFLCGQGYSTAMVRRLSGDGSVCTLANSGRVWDLNYPSFALSTAPS-ESINQFF 668
           ++SD++ FLCG  Y++ +++ +SG+   C+ A      +LNYPS +   + S  +    F
Sbjct: 607 DKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTF 666

Query: 669 RRTLTNVESKASTYRYKVL-GAPQGLTITVDPPILSFNGIGQKESFTLTIRGTVRQSIV- 728
            RTLTNV +  STY  KV+ G    L + + P +LSF  + +K+SFT+T+ G+   S V 
Sbjct: 667 NRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVP 726

BLAST of Tan0005919 vs. TAIR 10
Match: AT5G59100.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 607.1 bits (1564), Expect = 1.9e-173
Identity = 336/746 (45.04%), Postives = 470/746 (63.00%), Query Frame = 0

Query: 8   MSSSLIFILVFLNLISNLLAS-SSDSDNDG-RKIYIVYLGNKPEDSASTP-SHHMRMLEE 67
           M+    FI +F +L+   L+S S+D D+ G +++YIVYLG+ P     TP S HM +L+E
Sbjct: 1   MAKGTTFIFLFSSLLVLSLSSVSADKDDHGDQQVYIVYLGSLPSREEYTPMSDHMSILQE 60

Query: 68  VVGSKFAPKSLLHSYKRSFNGFVVRLTEEEAQKISGKKGVVSVFPNEKKHLHTTRSWDFM 127
           + G       L+ SYK+SFNGF  RLTE E ++++G + VVSVFP+ K  L TT SW+FM
Sbjct: 61  ITGESLIENRLVRSYKKSFNGFAARLTESERKRLAGMERVVSVFPSRKLKLQTTSSWNFM 120

Query: 128 GFTKNV--PRVNQVESNIVVGVLDSGIWPESPSFSDVGFGLPPTKWKGTCQTSANFRCNR 187
           G  + +   R   +ES+ ++GV+DSGI+PES SFSD GFG PP KWKGTC    NF CN 
Sbjct: 121 GLKEGIKTKRTRSIESDTIIGVIDSGIYPESDSFSDQGFGPPPKKWKGTCAGGKNFTCNN 180

Query: 188 KIIGARAYRSDNFFPPEDIEGPRDSDGHGTHTASTVAGGLVDQASLFGLGLGTARGG--- 247
           K+IGAR Y + +    +  +  RD  GHGTHTAS  AG  V  ++ +GLG GTARGG   
Sbjct: 181 KVIGARDYTAKS----KANQTARDYSGHGTHTASIAAGNAVANSNFYGLGNGTARGGVPA 240

Query: 248 -------VCWSDGCHDADILAAFDDAIADGVDIISLSVGGNEPRFYFNDSIAIGAFHSMK 307
                  VC ++GC    +++AFDDAIADGVD+IS+S+  +    +  D IAIGAFH+M 
Sbjct: 241 ARIAVYKVCDNEGCDGEAMMSAFDDAIADGVDVISISIVLDNIPPFEEDPIAIGAFHAMA 300

Query: 308 HGILTSNSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSKLQLGNSKIYQGYTINTFDL 367
            G+LT N+AGN+GP   T+ + +PW  SVAAS  +R  ++K+ LG+ KI  G ++NT+D+
Sbjct: 301 VGVLTVNAAGNNGPKISTVTSTAPWVFSVAASVTNRAFMAKVVLGDGKILIGRSVNTYDM 360

Query: 368 LGKQYPLIYAGDALNVTGGF--TGLSSRKFSGSQSGEGKILLCDSILAPSTFASFSDAVG 427
            G  YPL+Y   A   T       L   K    +  +GKI+LCDS               
Sbjct: 361 NGTNYPLVYGKSAALSTCSVDKARLCEPKCLDGKLVKGKIVLCDSTKGLIEAQKLGAVGS 420

Query: 428 VVMNDAGVKDNSRSYPLPSSYLETADGDSIKSYMASNGVATVTIFKSDAVNDSSAPLIVS 487
           +V N    +   RS+P+  S+L   D  S+ SYM S      T+ KS+ +++  APL+ S
Sbjct: 421 IVKNPEPDRAFIRSFPV--SFLSNDDYKSLVSYMNSTKNPKATVLKSEEISNQRAPLVAS 480

Query: 488 FSSRGPNPETLDILKPDLTAPGVEILAAWSPNASVSSGVRDSRKTMYNIISGTSMSCPHA 547
           FSSRGP+    DILKPD+TAPGVEILAA+SP++S +    D+R+  Y+++SGTSM+CPH 
Sbjct: 481 FSSRGPSSIVSDILKPDITAPGVEILAAYSPDSSPTESEFDTRRVKYSVLSGTSMACPHV 540

Query: 548 TAAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELN--PEAEFAYGAGHIDPLKAINPGLV 607
              A YVKTFHP WSP+ I+SA+MTTA P+NA  +     EFAYG+GH+DP+ AINPGLV
Sbjct: 541 AGVAAYVKTFHPQWSPSMIQSAIMTTAWPMNASGSGFVSTEFAYGSGHVDPIDAINPGLV 600

Query: 608 YDANESDYVKFLCGQGYSTAMVRRLSGDGSVCTLANSGRV-WDLNYPSFALSTAPSESIN 667
           Y+  ++D++ FLCG  Y++  +R +SGD S CT   S  +  +LNYP+ +   + ++  N
Sbjct: 601 YELTKADHINFLCGLNYTSDHLRIISGDNSTCTKEISKTLPRNLNYPTMSAKVSGTKPFN 660

Query: 668 QFFRRTLTNVESKASTYRYKVLGAP-QGLTITVDPPILSFNGIGQKESFTLTIRGTV--R 727
             F+RT+TNV  + STY  KV+  P   L+I V P +LS   + +K+SF +T+       
Sbjct: 661 ITFQRTVTNVGMQKSTYNAKVVKFPGSKLSIKVSPRVLSMKSMNEKQSFMVTVSSDSIGT 720

Query: 728 QSIVSASLVWTDGSHNVRSPITVYVV 731
           +  VSA+L+W+DG+HNVRSPI VY +
Sbjct: 721 KQPVSANLIWSDGTHNVRSPIIVYAM 740

BLAST of Tan0005919 vs. TAIR 10
Match: AT5G59090.1 (subtilase 4.12 )

HSP 1 Score: 605.5 bits (1560), Expect = 5.6e-173
Identity = 333/733 (45.43%), Postives = 459/733 (62.62%), Query Frame = 0

Query: 21  LISNLLASSSDSDNDGRKIYIVYLGNKPEDSASTP-SHHMRMLEEVVGSKFAPKSLLHSY 80
           L+  LL+S S   ++  ++YIVY+G+    +   P S HM +L++V G       L+ SY
Sbjct: 14  LLVLLLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHMSILQQVTGESSIEGRLVRSY 73

Query: 81  KRSFNGFVVRLTEEEAQKISGKKGVVSVFPNEKKHLHTTRSWDFMGFT--KNVPRVNQVE 140
           KRSFNGF  RLTE E   I+  +GVVSVFPN+   LHTT SWDFMG    KN  R   +E
Sbjct: 74  KRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNTKRNLAIE 133

Query: 141 SNIVVGVLDSGIWPESPSFSDVGFGLPPTKWKGTCQTSANFRCNRKIIGARAYRSDNFFP 200
           S+ ++GV+D+GIWPES SFSD GFG PP KWKG C    NF CN K+IGAR Y S     
Sbjct: 134 SDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDYTS----- 193

Query: 201 PEDIEGPRDSDGHGTHTASTVAGGLVDQASLFGLGLGTARGG----------VCWSDGCH 260
               EG RD+ GHGTHTAST AG  V   S FG+G GT RGG          VC   GC 
Sbjct: 194 ----EGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKVCTDSGCS 253

Query: 261 DADILAAFDDAIADGVDIISLSVGGNEPRFYFNDSIAIGAFHSMKHGILTSNSAGNDGPD 320
              +L++FDDAIADGVD+I++S+G   P  + +D IAIGAFH+M  GILT +SAGN GP 
Sbjct: 254 SEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSSAGNSGPK 313

Query: 321 YFTIRNFSPWSLSVAASTIDRKLVSKLQLGNSKIYQGYTINTFDLLGKQYPLIYAGDALN 380
             T+ + +PW  +VAAST +R  ++K+ LGN K   G ++N FD+ GK+YPL+Y   A +
Sbjct: 314 PTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLVYGKSAAS 373

Query: 381 --VTGGFTGLSSRKFSGSQSGEGKILLCDSILAPSTFASFSDAVGVVMNDAGVK-DNSRS 440
                    L +         +GKIL+C     PS +        + + D   + D + +
Sbjct: 374 SACDAKTAALCAPACLNKSRVKGKILVCG---GPSGYKIAKSVGAIAIIDKSPRPDVAFT 433

Query: 441 YPLPSSYLETADGDSIKSYMASNGVATVTIFKSDAVNDSSAPLIVSFSSRGPNPETLDIL 500
           + LP+S L+  D  S+ SY+ S       + K++ + + ++P+I SFSSRGPN   +DIL
Sbjct: 434 HHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTIAVDIL 493

Query: 501 KPDLTAPGVEILAAWSPNASVSSGVRDSRKTMYNIISGTSMSCPHATAAAVYVKTFHPTW 560
           KPD+TAPGVEILAA+SPN   S    D+R+  Y++ SGTSM+CPH    A YVKTF+P W
Sbjct: 494 KPDITAPGVEILAAFSPNGEPSED--DTRRVKYSVFSGTSMACPHVAGVAAYVKTFYPRW 553

Query: 561 SPAAIKSALMTTAIPLNAELN--PEAEFAYGAGHIDPLKAINPGLVYDANESDYVKFLCG 620
           SP+ I+SA+MTTA P+ A+       EFAYGAGH+DP+ A+NPGLVY+ +++D++ FLCG
Sbjct: 554 SPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFLCG 613

Query: 621 QGYSTAMVRRLSGDGSVCTLANSGRVWDLNYPSFALSTAPSES-INQFFRRTLTNVESKA 680
             Y++  ++ +SGD   C+  N     +LNYPS +   + ++S  +  F RTLTNV +  
Sbjct: 614 MNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGTPN 673

Query: 681 STYRYKVL-GAPQGLTITVDPPILSFNGIGQKESFTLTIRGTVRQSIV--SASLVWTDGS 732
           STY+ KV+ G    L+I V P +L F  + +K+SF++T+ G+   S V  SA+L+W+DG+
Sbjct: 674 STYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSDGT 732

BLAST of Tan0005919 vs. TAIR 10
Match: AT5G58840.1 (Subtilase family protein )

HSP 1 Score: 604.7 bits (1558), Expect = 9.5e-173
Identity = 344/743 (46.30%), Postives = 452/743 (60.83%), Query Frame = 0

Query: 14  FILVFLNLISNLLA-SSSDSDNDGRKIYIVYLGNKPEDSAS-TP-SHHMRMLEEVVGSKF 73
           F L+   L+S +++ S+   D+  +++Y+VY+G+ P      TP SHHM +L+EV G   
Sbjct: 8   FCLISCVLVSFVISVSAVTDDSQDKQVYVVYMGSLPSSRLEYTPMSHHMSILQEVTGESS 67

Query: 74  APKSLLHSYKRSFNGFVVRLTEEEAQKISGKKGVVSVFPNEKKHLHTTRSWDFMGFT--K 133
               L+ SYKRSFNGF  RLTE E ++++  +GVVSVFP+    L TT SWDF+G    K
Sbjct: 68  VEGRLVRSYKRSFNGFAARLTESERERVAEMEGVVSVFPDINYKLQTTASWDFLGLKEGK 127

Query: 134 NVPRVNQVESNIVVGVLDSGIWPESPSFSDVGFGLPPTKWKGTCQTSANFRCNRKIIGAR 193
           N  R   +ES+ ++G +DSGIWPES SFSD GFG PP KWKG C    NF CN K+IGAR
Sbjct: 128 NTKRNLAIESDTIIGFIDSGIWPESESFSDKGFGPPPKKWKGVCSAGKNFTCNNKLIGAR 187

Query: 194 AYRSDNFFPPEDIEGPRDSDGHGTHTASTVAGGLVDQASLFGLGLGTARGGV-------- 253
            Y +         EG RD +GHGTHTAST AG  V   S +G+G GTARGGV        
Sbjct: 188 DYTN---------EGTRDIEGHGTHTASTAAGNAVKNTSFYGIGNGTARGGVPASRIAAY 247

Query: 254 --CWSDGCHDADILAAFDDAIADGVDIISLSVGGNEPRFYFNDSIAIGAFHSMKHGILTS 313
             C   GC    +L+AFDDAIADGVD+IS+S+G N  R Y  D IAIGAFH+M  GILT 
Sbjct: 248 KACSEMGCTTESVLSAFDDAIADGVDLISISLGANLVRTYETDPIAIGAFHAMVKGILTV 307

Query: 314 NSAGNDGPDYFTIRNFSPWSLSVAASTIDRKLVSKLQLGNSKIYQGYTINTFDLLGKQYP 373
            SAGN GP+  ++ + +PW L+VAAS  +R  V+K+ LGN K + G ++N FDL GK YP
Sbjct: 308 QSAGNGGPNPGSVMSVAPWILTVAASNTNRGFVTKVVLGNGKTFVGKSLNAFDLKGKNYP 367

Query: 374 LIYAGDALNVTGGFTGLSSRKFSGSQSG---EGKILLCDSILAPSTFASFSDAVGVVMND 433
           L                    + GS  G    GKIL+ +            D V   +  
Sbjct: 368 L--------------------YGGSTDGPLLRGKILVSE------------DKVSSEIVV 427

Query: 434 AGVKDNSRSYP----LPSSYLETADGDSIKSYMASNGVATVTIFKSDAVNDSSAPLIVSF 493
           A + +N   Y     LPSS L   D DS+ SY+ S      T+ KS+A+ + +AP +  F
Sbjct: 428 ANINENYHDYAYVSILPSSALSKDDFDSVISYVNSTKSPHGTVLKSEAIFNQAAPKVAGF 487

Query: 494 SSRGPNPETLDILKPDLTAPGVEILAAWSPNASVSSGVRDSRKTMYNIISGTSMSCPHAT 553
           SSRGPN   +DILKPD+TAPGVEILAA+SP  S +   RD+R   Y+++SGTSMSCPH  
Sbjct: 488 SSRGPNTIAVDILKPDVTAPGVEILAAFSPLNSPAQDKRDNRHVKYSVLSGTSMSCPHVA 547

Query: 554 AAAVYVKTFHPTWSPAAIKSALMTTAIPLNAELNPEA--EFAYGAGHIDPLKAINPGLVY 613
             A Y+KTFHP WSP+ I+SA+MTTA P+NA     A  EFAYGAGH+DP+ AINPGLVY
Sbjct: 548 GVAAYIKTFHPEWSPSMIQSAIMTTAWPMNATGTAVASTEFAYGAGHVDPIAAINPGLVY 607

Query: 614 DANESDYVKFLCGQGYSTAMVRRLSGDGSVCTLANSGRVWDLNYPSFALSTAPSE-SINQ 673
           +  +SD++ FLCG  Y+   ++ ++G+   CT     R  +LNYPS +     SE S   
Sbjct: 608 EIGKSDHIAFLCGLNYNATSLKLIAGEAVTCTGKTLPR--NLNYPSMSAKLPKSESSFIV 667

Query: 674 FFRRTLTNVESKASTYRYK-VLGAPQGLTITVDPPILSFNGIGQKESFTLTIRGTVRQSI 729
            F RT+TNV +  STY+ K VL     L + V P +LS   + +K+SFT+T+ G+     
Sbjct: 668 TFNRTVTNVGTPNSTYKSKIVLNHGSNLKVEVSPSVLSMKSVKEKQSFTVTVSGSNIDPK 707