Tan0005604 (gene) Snake gourd v1

Overview
NameTan0005604
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionheat stress transcription factor B-4-like
LocationLG01: 113483497 .. 113487480 (+)
RNA-Seq ExpressionTan0005604
SyntenyTan0005604
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACACACACACTTCCTTTCCTTTCTTCCGCTTTGTTTTTCCTTCTCTCTCTCTAAAGTCTAGCTAAACATTTGATTTGATCCGCCATTCCCCATTAATTTCACACACTAGTCCCCTATCTTTCTCATCCATATCTGTTTTCAAACCCCACCCAAATAGACTCTTCCTTTTCTCTCAGATCTCAGCCATTCCAATATCAATAATGGCTCTAATGCTTGATAATTGCGAAGGCGTTTTGCTTTCCTTAGACTCCCACAAAGCAATCCCAGCTCCATTTCTTACTAAAACTTACCAGCTCGTTGACGATCCCGCCACCGACCATATTGTCTCATGGGGCGAAGATGATACCACCTTCGTTGTTTGGCGCCCTCCTGAATTTGCTAGAGATCTCCTTCCTAACTATTTCAAGCACAACAACTTCTCTAGCTTCGTCCGCCAGCTCAACACCTATGTAAGAATTTCATTTTTATGTCAGATCCTTTCCTCTTTTTCTTTTTTAATTCCTTTTACAAAAATTTAATTTTACTCCTTTTCTTTAGGGTTTTAGAAAAATTGTGCCGGATAGATGGGAATTTGCGAATGAGTTCTTCAGAAAAGGAGAGAAACATTTGTTATGTGAGATCCATCGACGAAAAACCGCTCAGCCTCAACTCACCGTCAACCAACATCACCAATCTCATTCTCCAGTACTCGGTGTCAACAATCCCAGTTTCTACCATTTTCCCGCCCGACTCAGCATCTCCCCGTCCGACTCCGACGACAACAACCCCAATTGGTGTGACTCGCCACCTCTCTCCTCCCCCGGAGGTGTCGCTGCACCCAACTTCAACAACTCCGTCACCGCCTTGTCGGAAGACAACGAGCGTCTTCGTCGGAGCAACAACATGCTCATGTCCGAATTAGCCCACATGAAAAAACTCTACAACGACATCATCTATTTCGTTCAAAACCACGTCAAGCCCGTTGCCCCGAGTAATTCATATCAATATTCAACGTCTCTACTTTCCGACGGTTTTCCGGTGGTGCAACGGCCGCCGAATCACCACCTTCTCGGGTACCATCCGACGAACGCGAAACAAGTTTCCGGGCAGACCCATTTGATGAGCGGAACTCCAAATAGTAATAATAATAATAATACTAATAATAATGGTTCCTCAAAAAGCTTCGTGACGATTCTAGAAGAACCCAAGACGAAGCTTTTTGGAGTGGCGATTCAATCGAAGAAACGGCTGCACCCGGAGTATGGTTCTAATAATATCGGCAAAGAGAACAACAAGGCTCGATTGGTGTTGGAAAAAGATGATTTGGGCCTCAATCTCATGCCCCCTTCCGCTTGTTAGTTCTTCTTCCTCTTCTAATCCTCATATTCCTTTCATTTACAGAAAAAAAAAAAAATGTTGATTATCCTTTGTTTTTTTTTTTTTTGGGAATTTTTGTTAGTCTCTCTTACAAGTCTGAGAGGGGATCCTTTTCCAGTGCTGCATTGATCATAATTGAATTAGAATATGTGTGCGTGCGTATGGTTGTTTGTGATAGTAGTGTAAGTTTCTATGTATATAACCCTCAGTGACGCTATTTCTGTCTCTTTTCCCCATAACCATTATTGTTGCACTGGAACTCTCGAATCTGGACTCTCTCTTTCTCTCTCTGCTTGTAGAGAAGAAGAAAAATTAGTTTTTTTTTTTTCAATTAAATTCCATCTTTTTCACGATCTCCTTTTTAACTTCTCTTCCGTATTCCATTGCTTGTTATATTATTATTATTATTTTTTTTTTTTGTGGGTGTTTGTGTTTAATGGGTTTGACCTGGAATATTTCAGTGTGACATTAATGTGGGATAGTGATCAGTGAACGAGTGAGATATAATGAGGGGAGTACGAAAAGGGTTCTTAAATGGAAAGTTTTTTCCTTTTCATTTCAGTTCTACACAGAAAGAAGGGCAAAATATTTTTGAAAATATTTGTATCAGAAGAAGATAAAGAGATGAGAAAGACCGTGTAATTAATTATTAAATAAATAAGCTGTGCTGCTGACACGCAGGAGAAGGGAAAAACCAAAAAAAAAAGAAAAAAAAAGGTCAGTTTGTTCTTCGACCAGCTTCAATTATTTAATAATTGCATTCACTATTGTAATTTATTATCATACTTAATTAAATATTTTGTCAGGGCCCAAAATTTTTGTATATGATTTAATCAGAAAGAAGGCTATTATATCATTTGATTCATGTTGGCCGTATTTTACTAATCAATCTCTCTTTCTCTTCTTTCAATGGAAAGCCTAATTAAACAATGATTCCCCAACATTGATCTTGTTCTTTCTCGTCATATGTATATATATATTCTTAATGTTTTTAATTAATTATTAAAAAGAGAGAGAGAGAGAGAGAAGGGAGGTGTGTAAATTAGATGTGGGGTTTGAAGTTTGAAGGGGAAGGGGAAGAAATAAAAACCTTAGCTTTTTCCTACTTAAAGTATGAGATGGAATGAGTTGAAAACCCTAGGGGCTTAGAACACTCCCTTCTCTTTCAGGTACAAATAGAGCTTTGTGAGTTGTTGCTTCGGACCAGCTAGAGTGATGATTGACTGGTAGATAAACCAACCCACTTTGCCGAAATGCCAGTCTCTTTTATGGTTTCCCTTTTGTCTCTTTTTGCAGAAACTCCATTACGGGTATATATATATATATATATAATTATTTTTATTATTTTTTTTTTCTATCAATCGGAAAGTTTGACCATGGCAGGCCAATGTGTAACTCTGTGCCTCTATTGCTTTCTCGTTTCATAAATTTGTGTCTTTCATGCGGGCATTTAATTTCATGACAGAAAAAACAAAAAAAATAAAAATAAATAAATAAAATAAAACACAGCTGTGTGAAACGCAGAGTTTTATCTCTAAAGAAAAAGATCTTTTTTTTTTTCTTTTTAAATAATAGTTCTCTAGTTCAAGGGTAGTAAAAGTTTGGAATATTATAGGCCTTGGGATATCTTTGTCGTGCAATGATTTCCTCTCTCACACATAATTTCAAAAGTAAACCAGTTGTGTGTGTGGTGTAGATATAATAAAAATAATAATAATATGTAAAAAGAAATTAAATATATAAAAAGACGCAACAACAAAACCCTTATTCTATGATACCTTTGTTTAATGTTTACAGTCCTGACTGAAGCTGTTGTCTTTTCTCACGGGGGGCTCTCACGTGACTCGGATGTGCACGTGAAGCCTAAAACGATGCAGTTGGGTTGGGGGGTTTTGGGAAAGAAAAAAAGGATAATAAAGGGAAATAAGAAACCTCCATTAAATAAAAATATGCCTTAAAGTTTAAACTACCAATTTATTTTTCATTGCCAAACAGCCGTATTCAACCCATGGGAGATGATTCTTTGGAACCTCTAAAGTAAAAGGGAAAATATTAAATTTGTTGTGTGACAGTTTTCTTTTTTTCTTTTTTTTTTTCCCTTTTGAAATTAATTTTCCTTTAGTAAAGAGATGCATGCCCACTTTGTCTGAACCAACTTTGTTGACATTTTTTTCCCTTTCATTTTCTTTTGATACTTCCCTTTATGCATGTAATTTCTTTATAATTAAATGTATAAGGTTGGATAAGCCGCATGGTATCAAAGGAAGATACTGATTTGTTAGTTTTAGCTTTTTTTATAATTTATACATTTAAGTCAAGATTTCTTTACTATTTGAGATCCACCCCTACAATAAGAGTCAAAAAGCTATAGAAAGAAAATTCCGCACGTCAGTCATAATTACTTTTTAAATTGAAAAAGATTTTTTTCCCCCTTCTTTTTTTAGCTATTGAAAATACTTTTAATTAAATTGACATGTTGACATTATGTATTTGACTGATGCATGCAGGTTTTCCCTGTGGTATTGAACTCGAAAGAAAAAGGAAAGGTTTTCCTCTGATATTAAAGTTGGCAGAAAATTCAGCCCAACATGGGCTGCAGAAATGGGAAGGAATGATTAAGCCCACCC

mRNA sequence

CACACACACACTTCCTTTCCTTTCTTCCGCTTTGTTTTTCCTTCTCTCTCTCTAAAGTCTAGCTAAACATTTGATTTGATCCGCCATTCCCCATTAATTTCACACACTAGTCCCCTATCTTTCTCATCCATATCTGTTTTCAAACCCCACCCAAATAGACTCTTCCTTTTCTCTCAGATCTCAGCCATTCCAATATCAATAATGGCTCTAATGCTTGATAATTGCGAAGGCGTTTTGCTTTCCTTAGACTCCCACAAAGCAATCCCAGCTCCATTTCTTACTAAAACTTACCAGCTCGTTGACGATCCCGCCACCGACCATATTGTCTCATGGGGCGAAGATGATACCACCTTCGTTGTTTGGCGCCCTCCTGAATTTGCTAGAGATCTCCTTCCTAACTATTTCAAGCACAACAACTTCTCTAGCTTCGTCCGCCAGCTCAACACCTATGGTTTTAGAAAAATTGTGCCGGATAGATGGGAATTTGCGAATGAGTTCTTCAGAAAAGGAGAGAAACATTTGTTATGTGAGATCCATCGACGAAAAACCGCTCAGCCTCAACTCACCGTCAACCAACATCACCAATCTCATTCTCCAGTACTCGGTGTCAACAATCCCAGTTTCTACCATTTTCCCGCCCGACTCAGCATCTCCCCGTCCGACTCCGACGACAACAACCCCAATTGGTGTGACTCGCCACCTCTCTCCTCCCCCGGAGGTGTCGCTGCACCCAACTTCAACAACTCCGTCACCGCCTTGTCGGAAGACAACGAGCGTCTTCGTCGGAGCAACAACATGCTCATGTCCGAATTAGCCCACATGAAAAAACTCTACAACGACATCATCTATTTCGTTCAAAACCACGTCAAGCCCGTTGCCCCGAGTAATTCATATCAATATTCAACGTCTCTACTTTCCGACGGTTTTCCGGTGGTGCAACGGCCGCCGAATCACCACCTTCTCGGGTACCATCCGACGAACGCGAAACAAGTTTCCGGGCAGACCCATTTGATGAGCGGAACTCCAAATAGTAATAATAATAATAATACTAATAATAATGGTTCCTCAAAAAGCTTCGTGACGATTCTAGAAGAACCCAAGACGAAGCTTTTTGGAGTGGCGATTCAATCGAAGAAACGGCTGCACCCGGAGTATGGTTCTAATAATATCGGCAAAGAGAACAACAAGGCTCGATTGGTGTTGGAAAAAGATGATTTGGGCCTCAATCTCATGCCCCCTTCCGCTTGTTAGTTCTTCTTCCTCTTCTAATCCTCATATTCCTTTCATTTACAGAAAAAAAAAAAAATGTTGATTATCCTTTGTTTTTTTTTTTTTTGGGAATTTTTGTTAGTCTCTCTTACAAGTCTGAGAGGGGATCCTTTTCCAGTGCTGCATTGATCATAATTGAATTAGAATATGTGTGCGTGCGTATGGTTGTTTGTGATAGTAGTGTAAGTTTCTATGTATATAACCCTCAGTGACGCTATTTCTGTCTCTTTTCCCCATAACCATTATTGTTGCACTGGAACTCTCGAATCTGGACTCTCTCTTTCTCTCTCTGCTTGTAGAGAAGAAGAAAAATTAGTTTTTTTTTTTTCAATTAAATTCCATCTTTTTCACGATCTCCTTTTTAACTTCTCTTCCGTATTCCATTGCTTGTTATATTATTATTATTATTTTTTTTTTTTGTGGGTGTTTGTGTTTAATGGGTTTGACCTGGAATATTTCAGTGTGACATTAATGTGGGATAGTGATCAGTGAACGAGTGAGATATAATGAGGGGAGTACGAAAAGGGTTCTTAAATGGAAAGTTTTTTCCTTTTCATTTCAGTTCTACACAGAAAGAAGGGCAAAATATTTTTGAAAATATTTGTATCAGAAGAAGATAAAGAGATGAGAAAGACCGTGTAATTAATTATTAAATAAATAAGCTGTGCTGCTGACACGCAGGAGAAGGGAAAAACCAAAAAAAAAAGAAAAAAAAAGGTTTTCCCTGTGGTATTGAACTCGAAAGAAAAAGGAAAGGTTTTCCTCTGATATTAAAGTTGGCAGAAAATTCAGCCCAACATGGGCTGCAGAAATGGGAAGGAATGATTAAGCCCACCC

Coding sequence (CDS)

ATGGCTCTAATGCTTGATAATTGCGAAGGCGTTTTGCTTTCCTTAGACTCCCACAAAGCAATCCCAGCTCCATTTCTTACTAAAACTTACCAGCTCGTTGACGATCCCGCCACCGACCATATTGTCTCATGGGGCGAAGATGATACCACCTTCGTTGTTTGGCGCCCTCCTGAATTTGCTAGAGATCTCCTTCCTAACTATTTCAAGCACAACAACTTCTCTAGCTTCGTCCGCCAGCTCAACACCTATGGTTTTAGAAAAATTGTGCCGGATAGATGGGAATTTGCGAATGAGTTCTTCAGAAAAGGAGAGAAACATTTGTTATGTGAGATCCATCGACGAAAAACCGCTCAGCCTCAACTCACCGTCAACCAACATCACCAATCTCATTCTCCAGTACTCGGTGTCAACAATCCCAGTTTCTACCATTTTCCCGCCCGACTCAGCATCTCCCCGTCCGACTCCGACGACAACAACCCCAATTGGTGTGACTCGCCACCTCTCTCCTCCCCCGGAGGTGTCGCTGCACCCAACTTCAACAACTCCGTCACCGCCTTGTCGGAAGACAACGAGCGTCTTCGTCGGAGCAACAACATGCTCATGTCCGAATTAGCCCACATGAAAAAACTCTACAACGACATCATCTATTTCGTTCAAAACCACGTCAAGCCCGTTGCCCCGAGTAATTCATATCAATATTCAACGTCTCTACTTTCCGACGGTTTTCCGGTGGTGCAACGGCCGCCGAATCACCACCTTCTCGGGTACCATCCGACGAACGCGAAACAAGTTTCCGGGCAGACCCATTTGATGAGCGGAACTCCAAATAGTAATAATAATAATAATACTAATAATAATGGTTCCTCAAAAAGCTTCGTGACGATTCTAGAAGAACCCAAGACGAAGCTTTTTGGAGTGGCGATTCAATCGAAGAAACGGCTGCACCCGGAGTATGGTTCTAATAATATCGGCAAAGAGAACAACAAGGCTCGATTGGTGTTGGAAAAAGATGATTTGGGCCTCAATCTCATGCCCCCTTCCGCTTGTTAG

Protein sequence

MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPATDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQLTVNQHHQSHSPVLGVNNPSFYHFPARLSISPSDSDDNNPNWCDSPPLSSPGGVAAPNFNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSDGFPVVQRPPNHHLLGYHPTNAKQVSGQTHLMSGTPNSNNNNNTNNNGSSKSFVTILEEPKTKLFGVAIQSKKRLHPEYGSNNIGKENNKARLVLEKDDLGLNLMPPSAC
Homology
BLAST of Tan0005604 vs. ExPASy Swiss-Prot
Match: Q9C635 (Heat stress transcription factor B-4 OS=Arabidopsis thaliana OX=3702 GN=HSFB4 PE=2 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 1.7e-90
Identity = 200/375 (53.33%), Postives = 241/375 (64.27%), Query Frame = 0

Query: 1   MALMLDNCEG----------VLLSLDSHKAIPAPFLTKTYQLVDDPATDHIVSWGEDDTT 60
           MA+M++N  G           L+     KA+PAPFLTKTYQLVDDPATDH+VSWG+DDTT
Sbjct: 1   MAMMVENSYGGYGGGGGERIQLMVEGQGKAVPAPFLTKTYQLVDDPATDHVVSWGDDDTT 60

Query: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCE 120
           FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFF++GEKHLLCE
Sbjct: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFKRGEKHLLCE 120

Query: 121 IHRRKTAQPQLTVNQH-----HQSHSPVLGVNNPSFYHFPARLSISPSDSDDNNPNWCDS 180
           IHRRKT+  Q+   QH     H    P +  +  SF+  P     +P    + +  WCD 
Sbjct: 121 IHRRKTS--QMIPQQHSPFMSHHHAPPQIPFSGGSFFPLPPPRVTTP----EEDHYWCDD 180

Query: 181 PPLSSPGGVAAP-NFNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKP 240
            P S P  +    +    VTALSEDNERLRRSN +LMSELAHMKKLYNDIIYFVQNHVKP
Sbjct: 181 SPPSRPRVIPQQIDTAAQVTALSEDNERLRRSNTVLMSELAHMKKLYNDIIYFVQNHVKP 240

Query: 241 VAPSNSYQYSTSLLSDGFPVVQRPPNHHLLGYHPTNAKQVSGQTHLMSGTPNSNNNNNTN 300
           VAPSN+  Y +S L       Q+PP    L Y+ T              T N+ N N  N
Sbjct: 241 VAPSNNSSYLSSFLQK--QQQQQPPT---LDYYNT-------------ATVNATNLNALN 300

Query: 301 NN-GSSKSFVTILEEP-----------KTKLFGVAIQSKKRLHPEYGSNNIGKENNKARL 348
           ++  +S+S +T+LE+            KTKLFGV++ S K+      S++   + +K RL
Sbjct: 301 SSPPTSQSSITVLEDDHTNHHDQSNMRKTKLFGVSLPSSKK-----RSHHFSDQTSKTRL 346

BLAST of Tan0005604 vs. ExPASy Swiss-Prot
Match: Q7XHZ0 (Heat stress transcription factor B-4b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4B PE=1 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 7.5e-70
Identity = 142/252 (56.35%), Postives = 169/252 (67.06%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLD----------SHKAIPAPFLTKTYQLVDDPATDHIVSWGEDDTT 60
           MA +++ C  +++S++          + K +PAPFLTKTYQLVDDP TDHIVSWGEDDTT
Sbjct: 1   MAFLVERCGEMVVSMEMGPHGGGGAAAGKPVPAPFLTKTYQLVDDPCTDHIVSWGEDDTT 60

Query: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCE 120
           FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIV DRWEFANEFFRKG KHLL E
Sbjct: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVADRWEFANEFFRKGAKHLLAE 120

Query: 121 IHRRKTAQP-----QLTVNQHHQSHSPVLGVNNPSFYHFPARLSISPSDSDDNNPNWCDS 180
           IHRRK++QP           HH   +P      P  YH    +   P+ +       C  
Sbjct: 121 IHRRKSSQPPPPPMPHQPYHHHHHLNPFSLPPPPPAYHHHHLIQEEPATTAH-----CTV 180

Query: 181 PPLSSPGGVAAPNFNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPV 238
                 GG       + + ALSEDN +LRR N++L+SELAHMKKLYNDIIYF+QNHV PV
Sbjct: 181 AGDGGEGG-------DFLAALSEDNRQLRRRNSLLLSELAHMKKLYNDIIYFLQNHVAPV 240

BLAST of Tan0005604 vs. ExPASy Swiss-Prot
Match: Q67U94 (Heat stress transcription factor B-4c OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4C PE=2 SV=1)

HSP 1 Score: 259.6 bits (662), Expect = 5.4e-68
Identity = 171/381 (44.88%), Postives = 210/381 (55.12%), Query Frame = 0

Query: 7   NCEGVLLSLDSHKAIPAPFLTKTYQLVDDPATDHIVSWGEDD-TTFVVWRPPEFARDLLP 66
           +CE    +  + KA+PAPFLTKTYQLVDDPATDHIVSWG+D  +TFVVWRPPEFARD+LP
Sbjct: 9   DCEAAAAA--AQKAVPAPFLTKTYQLVDDPATDHIVSWGDDRVSTFVVWRPPEFARDILP 68

Query: 67  NYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQLTVNQ 126
           NYFKHNNFSSFVRQLNTYGFRK+VP+RWEFANEFFRKGEK LL EIHRRKT+    + + 
Sbjct: 69  NYFKHNNFSSFVRQLNTYGFRKVVPERWEFANEFFRKGEKQLLTEIHRRKTS----SAST 128

Query: 127 HHQSHSPVLGVNNPSFYHFPARLSISPSDS---DDN------------NPNWCD------ 186
              S  P     +   +H P   +     +   DD              P+W +      
Sbjct: 129 ASPSPPPFFAPPHFPLFHHPGVAAAQHHHAFVGDDGVVAAHGIGMPFPQPHWREPNLPVA 188

Query: 187 ------SPPLSSPGGVAAPNFNNSVTA--LSEDNERLRRSNNMLMSELAHMKKLYNDIIY 246
                   P  SP    A     + TA  L E+NERLRRSN  L+ ELAHM+KLYNDIIY
Sbjct: 189 TRLLALGGPAPSPSSAEAGGAGRAATAAVLMEENERLRRSNTALLQELAHMRKLYNDIIY 248

Query: 247 FVQNHVKPVAPSNSYQYSTSLLSDGFPVVQRPPNHHLLGYHPTNAKQVSGQT--HLMSGT 306
           FVQNHV+PVAPS +   +  L   G    ++P   ++L     +    S  T     S  
Sbjct: 249 FVQNHVRPVAPSPA--AAAFLQGLGMQARKKPAAANVLNNSGGSTTSSSSLTIAEEPSPP 308

Query: 307 PNSNNNNNTNNNGSSKSFVTILEEPKTKLFGVAIQ-------SKKRLHPEYGSNNIGKEN 349
           P   +     + G + +         TKLFGV +        SK+   PE   +      
Sbjct: 309 PQQQHLAGEKSGGEAGNSSAARSSAPTKLFGVHLSAAPCGAGSKRASSPE--EHPPTSPA 368

BLAST of Tan0005604 vs. ExPASy Swiss-Prot
Match: Q10KX8 (Heat stress transcription factor B-4d OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4D PE=3 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 1.6e-67
Identity = 141/255 (55.29%), Postives = 171/255 (67.06%), Query Frame = 0

Query: 1   MALMLDNCEG-VLLSLD-SH----------KAIPAPFLTKTYQLVDDPATDHIVSWGEDD 60
           MA +++ C G +++S++ SH           A PAPFL+KTYQLVDDP+TD +VSWGED+
Sbjct: 1   MAFLVERCGGEMVVSMERSHGRSTTTAAAVTAAPAPFLSKTYQLVDDPSTDDVVSWGEDE 60

Query: 61  TTFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLL 120
            TFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIV DRWEFANEFFRKG KHLL
Sbjct: 61  ATFVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVADRWEFANEFFRKGAKHLL 120

Query: 121 CEIHRRKTAQ-------PQLTVNQHH---------QSHSPVLGVNNPSFYHFPARLSISP 180
            EIHRRK++        P   ++QH+            SP +G    + YHF        
Sbjct: 121 SEIHRRKSSSCSQPQPPPPFPMHQHYPLSLFSPPTTPRSPPVGAAAAAAYHF-------- 180

Query: 181 SDSDDNNPNWCDSPPLSSPGGVAAPNFNNSVTALSEDNERLRRSNNMLMSELAHMKKLYN 228
                    +C SP   + GG       + + ALSEDN +LRR N++L+SELAHM+KLYN
Sbjct: 181 ------QEEYCSSPADYAGGG------GDLLAALSEDNRQLRRRNSLLLSELAHMRKLYN 235

BLAST of Tan0005604 vs. ExPASy Swiss-Prot
Match: Q6Z9R8 (Putative heat stress transcription factor B-4a OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB4A PE=3 SV=1)

HSP 1 Score: 237.7 bits (605), Expect = 2.2e-61
Identity = 136/266 (51.13%), Postives = 163/266 (61.28%), Query Frame = 0

Query: 17  SHKAIPAPFLTKTYQLVDDPATDHIVSWGEDD-----TTFVVWRPPEFARDLLPNYFKHN 76
           S   +PAPFLTKTYQLVDDPATDH+VSW +DD     ++FVVWRPPEFARD+LPNYFKH+
Sbjct: 18  SASVVPAPFLTKTYQLVDDPATDHVVSWEDDDGGESASSFVVWRPPEFARDILPNYFKHS 77

Query: 77  NFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTA----------QPQL 136
           NFSSFVRQLNTYGFRK+VP+RWEFANEFFRKGEK LLCEIHRRK+A           P  
Sbjct: 78  NFSSFVRQLNTYGFRKVVPERWEFANEFFRKGEKQLLCEIHRRKSAAATWPPFPPPPPPF 137

Query: 137 TVNQH--------------HQSHSPVLGVNNPSFYHFPARLSISPSDSDDNNPNWCDSPP 196
              +H              H     ++       +   A L ++PS           S  
Sbjct: 138 FAPRHFAAGAFFRHGDGMLHGRLGALVTTTERRHWFESAALPVAPS-----------SRL 197

Query: 197 LSSPGGVAAPNFNNSVT----ALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVK 250
           LS  G V AP    + T    AL ++N RL R N  L+ ELAHM+KLY+DIIYFVQNHV+
Sbjct: 198 LSQLGPVIAPARRAAATPEEEALMQENHRLLRGNAALVQELAHMRKLYSDIIYFVQNHVR 257

BLAST of Tan0005604 vs. NCBI nr
Match: XP_022940239.1 (heat stress transcription factor B-4-like [Cucurbita moschata] >XP_023525267.1 heat stress transcription factor B-4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 623.2 bits (1606), Expect = 1.4e-174
Identity = 313/348 (89.94%), Postives = 324/348 (93.10%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPATDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDP TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 LTVNQHHQSHSPVLGVNNPSFYHFPARLSISPSDSDDNNPNWCDSPPLSSPGGVAAPNFN 180
           +TVNQHHQS+SP LG+ N  FYH+PAR+SISPSDSDD NPNWCDSPPLSS       NFN
Sbjct: 121 VTVNQHHQSYSP-LGL-NAGFYHYPARVSISPSDSDD-NPNWCDSPPLSSNNN--NNNFN 180

Query: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSD 240
           NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSD
Sbjct: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSD 240

Query: 241 GFPVVQRPPNHHLLGYHPTNAKQVSGQTHLMSGTPNSNNNNNTNNNGSSKSFVTILEEPK 300
           GFPV QR PNHHLLGYHPTNAKQVSGQTH ++GTPN       NNNGSSKSFVTI+EEPK
Sbjct: 241 GFPVGQRQPNHHLLGYHPTNAKQVSGQTHFVAGTPN-------NNNGSSKSFVTIVEEPK 300

Query: 301 TKLFGVAIQSKKRLHPEYGSNNIGKENNKARLVLEKDDLGLNLMPPSA 349
           TKLFGV++QSKKR+HPEYGSNNI KENNKARLVLEKDDLGLNLMPPSA
Sbjct: 301 TKLFGVSLQSKKRVHPEYGSNNIVKENNKARLVLEKDDLGLNLMPPSA 336

BLAST of Tan0005604 vs. NCBI nr
Match: XP_022982267.1 (heat stress transcription factor B-4-like [Cucurbita maxima])

HSP 1 Score: 622.9 bits (1605), Expect = 1.8e-174
Identity = 313/348 (89.94%), Postives = 324/348 (93.10%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPATDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDP TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 LTVNQHHQSHSPVLGVNNPSFYHFPARLSISPSDSDDNNPNWCDSPPLSSPGGVAAPNFN 180
           +TVNQHHQS+SP LG+ N  FYH+PAR+SISPSDSDD NPNWCDSPPLSS        FN
Sbjct: 121 VTVNQHHQSYSP-LGL-NAGFYHYPARVSISPSDSDD-NPNWCDSPPLSSNNN-NNNVFN 180

Query: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSD 240
           NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSD
Sbjct: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSD 240

Query: 241 GFPVVQRPPNHHLLGYHPTNAKQVSGQTHLMSGTPNSNNNNNTNNNGSSKSFVTILEEPK 300
           GFPV QR PNHHLLGYHPTNAKQVSGQTH + GTPN+N     NNNGSSKSFVTI+EEPK
Sbjct: 241 GFPVGQRQPNHHLLGYHPTNAKQVSGQTHFVGGTPNNN-----NNNGSSKSFVTIVEEPK 300

Query: 301 TKLFGVAIQSKKRLHPEYGSNNIGKENNKARLVLEKDDLGLNLMPPSA 349
           TKLFGV++QSKKR+HPEYGSNNI KENNKARLVLEKDDLGLNLMPPSA
Sbjct: 301 TKLFGVSLQSKKRVHPEYGSNNIAKENNKARLVLEKDDLGLNLMPPSA 339

BLAST of Tan0005604 vs. NCBI nr
Match: KAG6608467.1 (Heat stress transcription factor B-4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 622.9 bits (1605), Expect = 1.8e-174
Identity = 313/350 (89.43%), Postives = 325/350 (92.86%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPATDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDP TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 LTVNQHHQSHSPVLGVNNPSFYHFPARLSISPSDSDDNNPNWCDSPPLSSPGGVA--APN 180
           +TVNQHHQS+SP LG+ N  FYH+PAR+SISPSDSDD NPNWCDSPPLSS    +    N
Sbjct: 121 VTVNQHHQSYSP-LGL-NAGFYHYPARVSISPSDSDD-NPNWCDSPPLSSNNNNSNNNNN 180

Query: 181 FNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLL 240
           FNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLL
Sbjct: 181 FNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLL 240

Query: 241 SDGFPVVQRPPNHHLLGYHPTNAKQVSGQTHLMSGTPNSNNNNNTNNNGSSKSFVTILEE 300
           SDGFPV QR PNHHLLGYHPTNAKQVSGQTH ++GTPN       NNNGSSKSFVTI+EE
Sbjct: 241 SDGFPVGQRQPNHHLLGYHPTNAKQVSGQTHFVAGTPN-------NNNGSSKSFVTIVEE 300

Query: 301 PKTKLFGVAIQSKKRLHPEYGSNNIGKENNKARLVLEKDDLGLNLMPPSA 349
           PKTKLFGV++QSKKR+HPEYGSNNI KENNKARLVLEKDDLGLNLMPPSA
Sbjct: 301 PKTKLFGVSLQSKKRVHPEYGSNNIVKENNKARLVLEKDDLGLNLMPPSA 340

BLAST of Tan0005604 vs. NCBI nr
Match: XP_038897232.1 (heat stress transcription factor B-4-like [Benincasa hispida])

HSP 1 Score: 577.4 bits (1487), Expect = 8.9e-161
Identity = 307/363 (84.57%), Postives = 315/363 (86.78%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPATDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 LTVNQHHQSHSPVLGVNNPSFYHFPARLSISPSDSDDNNPNWCDSPPLSSPGGVAAP--N 180
           +TVNQHHQSHSP LG+NNP FYHFP R+SISPSDSDD N NWCDSP LSSP    AP  N
Sbjct: 121 VTVNQHHQSHSP-LGINNPGFYHFPGRVSISPSDSDDQN-NWCDSPSLSSP----APHNN 180

Query: 181 FNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS-TSL 240
            NNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS TSL
Sbjct: 181 NNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTSL 240

Query: 241 LSDGFPVV-QRPPNHHLLGYHPTNAKQVSGQTHLMSGTPNSNNNNN--------TNNNGS 300
           LSD FPVV QRPPNHH   YH         Q HL++GT  +NNNNN         NNN  
Sbjct: 241 LSDRFPVVQQRPPNHHHHHYHQ------QSQIHLVTGTTPTNNNNNHNNNNNIIINNNSG 300

Query: 301 SKSFVTILEE--PKTKLFGVAIQSKKRLHPEYGSNNIGKE--NNKARLVLEKDDLGLNLM 348
           +KSFVTILEE   KTKLFGVAIQSKKRLHPEYGSNNIGKE  NNKARLVLE DDLGLNLM
Sbjct: 301 TKSFVTILEEQPTKTKLFGVAIQSKKRLHPEYGSNNIGKENNNNKARLVLENDDLGLNLM 351

BLAST of Tan0005604 vs. NCBI nr
Match: XP_023536624.1 (heat stress transcription factor B-4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 558.5 bits (1438), Expect = 4.3e-155
Identity = 293/351 (83.48%), Postives = 305/351 (86.89%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPATDHIVSWGEDDTTFVVWRPPEFA 60
           MALM+DNCEGVL+SLDSHK IPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMVDNCEGVLVSLDSHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFAN+FFRKG+KHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQPQ 120

Query: 121 LTVNQHHQSHSPVLGVNNPSFYHFPARLSISPSDSDDNNPNWCDSPPLSSPGGVAAPNFN 180
           L     HQS SP L +NNP FYHF  R SISPSDSDD N NWCDSPPLSS G     N N
Sbjct: 121 L-----HQSLSP-LALNNPGFYHFHGRGSISPSDSDDQN-NWCDSPPLSSSGTNNNNNNN 180

Query: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSD 240
           NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST     
Sbjct: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST----- 240

Query: 241 GFPVVQRPPNHHLLGY-HPTNAKQVSGQTHLMSGTPNSNNNNNTNNNGSSKSFVTILEE- 300
            FPVVQR PNHHL+GY +PTNAKQVSGQTHL++G PN+NN NN       KSFV I+EE 
Sbjct: 241 -FPVVQRQPNHHLVGYNNPTNAKQVSGQTHLVTGIPNNNNKNN-------KSFVRIVEEQ 300

Query: 301 PKTKLFGVAIQSKKRLHPEYGSNNIGKE-NNKARLVLEKDDLGLNLMPPSA 349
            KTKLFGVAI SKKRLHPEY SNNIGKE NNKAR VLEKDDLGLNLMPPSA
Sbjct: 301 QKTKLFGVAIHSKKRLHPEYASNNIGKENNNKARFVLEKDDLGLNLMPPSA 331

BLAST of Tan0005604 vs. ExPASy TrEMBL
Match: A0A6J1FJH2 (heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC111445920 PE=3 SV=1)

HSP 1 Score: 623.2 bits (1606), Expect = 6.9e-175
Identity = 313/348 (89.94%), Postives = 324/348 (93.10%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPATDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDP TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 LTVNQHHQSHSPVLGVNNPSFYHFPARLSISPSDSDDNNPNWCDSPPLSSPGGVAAPNFN 180
           +TVNQHHQS+SP LG+ N  FYH+PAR+SISPSDSDD NPNWCDSPPLSS       NFN
Sbjct: 121 VTVNQHHQSYSP-LGL-NAGFYHYPARVSISPSDSDD-NPNWCDSPPLSSNNN--NNNFN 180

Query: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSD 240
           NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSD
Sbjct: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSD 240

Query: 241 GFPVVQRPPNHHLLGYHPTNAKQVSGQTHLMSGTPNSNNNNNTNNNGSSKSFVTILEEPK 300
           GFPV QR PNHHLLGYHPTNAKQVSGQTH ++GTPN       NNNGSSKSFVTI+EEPK
Sbjct: 241 GFPVGQRQPNHHLLGYHPTNAKQVSGQTHFVAGTPN-------NNNGSSKSFVTIVEEPK 300

Query: 301 TKLFGVAIQSKKRLHPEYGSNNIGKENNKARLVLEKDDLGLNLMPPSA 349
           TKLFGV++QSKKR+HPEYGSNNI KENNKARLVLEKDDLGLNLMPPSA
Sbjct: 301 TKLFGVSLQSKKRVHPEYGSNNIVKENNKARLVLEKDDLGLNLMPPSA 336

BLAST of Tan0005604 vs. ExPASy TrEMBL
Match: A0A6J1IW73 (heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC111481146 PE=3 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 9.0e-175
Identity = 313/348 (89.94%), Postives = 324/348 (93.10%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPATDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDP TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPTTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 LTVNQHHQSHSPVLGVNNPSFYHFPARLSISPSDSDDNNPNWCDSPPLSSPGGVAAPNFN 180
           +TVNQHHQS+SP LG+ N  FYH+PAR+SISPSDSDD NPNWCDSPPLSS        FN
Sbjct: 121 VTVNQHHQSYSP-LGL-NAGFYHYPARVSISPSDSDD-NPNWCDSPPLSSNNN-NNNVFN 180

Query: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSD 240
           NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSD
Sbjct: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSD 240

Query: 241 GFPVVQRPPNHHLLGYHPTNAKQVSGQTHLMSGTPNSNNNNNTNNNGSSKSFVTILEEPK 300
           GFPV QR PNHHLLGYHPTNAKQVSGQTH + GTPN+N     NNNGSSKSFVTI+EEPK
Sbjct: 241 GFPVGQRQPNHHLLGYHPTNAKQVSGQTHFVGGTPNNN-----NNNGSSKSFVTIVEEPK 300

Query: 301 TKLFGVAIQSKKRLHPEYGSNNIGKENNKARLVLEKDDLGLNLMPPSA 349
           TKLFGV++QSKKR+HPEYGSNNI KENNKARLVLEKDDLGLNLMPPSA
Sbjct: 301 TKLFGVSLQSKKRVHPEYGSNNIAKENNKARLVLEKDDLGLNLMPPSA 339

BLAST of Tan0005604 vs. ExPASy TrEMBL
Match: A0A6J1F8Q8 (heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC111443150 PE=3 SV=1)

HSP 1 Score: 555.4 bits (1430), Expect = 1.8e-154
Identity = 292/351 (83.19%), Postives = 306/351 (87.18%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPATDHIVSWGEDDTTFVVWRPPEFA 60
           MALM+DNCEGVL+SL+SHK IPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMVDNCEGVLVSLESHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFAN+FFRKG+KHLLCEIHRRKTAQP 
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQP- 120

Query: 121 LTVNQHHQSHSPVLGVNNPSFYHFPARLSISPSDSDDNNPNWCDSPPLSSPGGVAAPNFN 180
               QH QS SP L +NNP FYHF AR SISPSDSDD N NWCDSPPLSS G     N N
Sbjct: 121 ----QHQQSLSP-LALNNPGFYHFHARPSISPSDSDDQN-NWCDSPPLSSSG---TNNNN 180

Query: 181 NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLLSD 240
           NSV+ALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST     
Sbjct: 181 NSVSALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST----- 240

Query: 241 GFPVVQRPPNHHLLGY-HPTNAKQVSGQTHLMSGTPNSNNNNNTNNNGSSKSFVTILEE- 300
            FPVVQR PNHHL+GY +PTNAKQVSGQTHL++ TPN+NNNNN       KSFV I+EE 
Sbjct: 241 -FPVVQRQPNHHLVGYNNPTNAKQVSGQTHLVTATPNNNNNNN-------KSFVRIVEEQ 300

Query: 301 PKTKLFGVAIQSKKRLHPEYGSNNIGKE-NNKARLVLEKDDLGLNLMPPSA 349
            KTKLFGVAI SKKRLHPEY SNNIGKE NNKAR VLEKDDLGLNLMPPSA
Sbjct: 301 QKTKLFGVAIHSKKRLHPEYASNNIGKENNNKARFVLEKDDLGLNLMPPSA 328

BLAST of Tan0005604 vs. ExPASy TrEMBL
Match: A0A6J1IFM1 (heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC111476507 PE=3 SV=1)

HSP 1 Score: 550.8 bits (1418), Expect = 4.3e-153
Identity = 289/353 (81.87%), Postives = 305/353 (86.40%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPATDHIVSWGEDDTTFVVWRPPEFA 60
           MALM+DNCEGVL+SL+ HK IPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMVDNCEGVLVSLEPHKPIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFAN+FFRKG+KHLLCEIHRRKTAQP 
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDFFRKGDKHLLCEIHRRKTAQP- 120

Query: 121 LTVNQHHQSHSPVLGVNNPSFYHFPARLSISPSDSDDNNPNWCDSPPLSSPG--GVAAPN 180
               QHHQS SP L +NNP FYHF  R SISPSDSDD N NWCDSPPLSS G       N
Sbjct: 121 ----QHHQSLSP-LALNNPGFYHFHGRGSISPSDSDDQN-NWCDSPPLSSSGTNNNNNNN 180

Query: 181 FNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTSLL 240
           ++NSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST   
Sbjct: 181 YSNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYST--- 240

Query: 241 SDGFPVVQRPPNHHLLGY-HPTNAKQVSGQTHLMSGTPNSNNNNNTNNNGSSKSFVTILE 300
              FPVVQR PNHHL+GY +PTNAK+VSGQTHL++GTPN       NNN S+KS V I+E
Sbjct: 241 ---FPVVQRQPNHHLVGYNNPTNAKRVSGQTHLVTGTPN-------NNNDSNKSLVRIVE 300

Query: 301 E-PKTKLFGVAIQSKKRLHPEYGSNNIGKE-NNKARLVLEKDDLGLNLMPPSA 349
           E  KTKLFGVAI SKKRLHPEY SNNIGKE NNKAR VLEKDDLGLNLMPPSA
Sbjct: 301 EQQKTKLFGVAIHSKKRLHPEYASNNIGKENNNKARFVLEKDDLGLNLMPPSA 333

BLAST of Tan0005604 vs. ExPASy TrEMBL
Match: A0A0A0L4W8 (HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639930 PE=3 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.6e-147
Identity = 282/356 (79.21%), Postives = 296/356 (83.15%), Query Frame = 0

Query: 1   MALMLDNCEGVLLSLDSHKAIPAPFLTKTYQLVDDPATDHIVSWGEDDTTFVVWRPPEFA 60
           MALMLD C+GVLLSLDSHKAIPAPFLTKTYQLVDDP+TDHIVSWGEDDTTFVVWRPPEFA
Sbjct: 1   MALMLDTCDGVLLSLDSHKAIPAPFLTKTYQLVDDPSTDHIVSWGEDDTTFVVWRPPEFA 60

Query: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120
           RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ
Sbjct: 61  RDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQ 120

Query: 121 LTVNQHHQSHSPVLGVNNPSFYHFP-ARLSISPSDSDDNNPNWCDSPPLSSPGGVAAPNF 180
           +TVNQHHQ HSP+    NP FYHFP ARLSISPSDSDD N  WCDSP   SP      N 
Sbjct: 121 VTVNQHHQPHSPL----NPGFYHFPTARLSISPSDSDDQNNYWCDSP---SPNN----NN 180

Query: 181 NNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS--TSL 240
           NNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYS  TSL
Sbjct: 181 NNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSYQYSTTTSL 240

Query: 241 LSDGFPVVQRPPNHHLLGYHPTNAKQVSGQTHLMSGTPNSNNNNNTNNNGSSKSFVTILE 300
           LSDGFPVV++P ++H   +H  + +QVS Q                NN   +KSFVTILE
Sbjct: 241 LSDGFPVVRQPNHYH---HHHHHHQQVSSQIR--------------NNTVGTKSFVTILE 300

Query: 301 E-----PKTKLFGVAIQSKKRLHPEYGSNNIGKENNKARLVLEKDDLGLNLMPPSA 349
           E      KTKLFGVAIQSKKRLHPEYG++N    NNKARLVLEKDDLGLNLMPPSA
Sbjct: 301 EEQQQQTKTKLFGVAIQSKKRLHPEYGNSNSNNNNNKARLVLEKDDLGLNLMPPSA 328

BLAST of Tan0005604 vs. TAIR 10
Match: AT1G46264.1 (heat shock transcription factor B4 )

HSP 1 Score: 334.3 bits (856), Expect = 1.2e-91
Identity = 200/375 (53.33%), Postives = 241/375 (64.27%), Query Frame = 0

Query: 1   MALMLDNCEG----------VLLSLDSHKAIPAPFLTKTYQLVDDPATDHIVSWGEDDTT 60
           MA+M++N  G           L+     KA+PAPFLTKTYQLVDDPATDH+VSWG+DDTT
Sbjct: 1   MAMMVENSYGGYGGGGGERIQLMVEGQGKAVPAPFLTKTYQLVDDPATDHVVSWGDDDTT 60

Query: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCE 120
           FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFF++GEKHLLCE
Sbjct: 61  FVVWRPPEFARDLLPNYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANEFFKRGEKHLLCE 120

Query: 121 IHRRKTAQPQLTVNQH-----HQSHSPVLGVNNPSFYHFPARLSISPSDSDDNNPNWCDS 180
           IHRRKT+  Q+   QH     H    P +  +  SF+  P     +P    + +  WCD 
Sbjct: 121 IHRRKTS--QMIPQQHSPFMSHHHAPPQIPFSGGSFFPLPPPRVTTP----EEDHYWCDD 180

Query: 181 PPLSSPGGVAAP-NFNNSVTALSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKP 240
            P S P  +    +    VTALSEDNERLRRSN +LMSELAHMKKLYNDIIYFVQNHVKP
Sbjct: 181 SPPSRPRVIPQQIDTAAQVTALSEDNERLRRSNTVLMSELAHMKKLYNDIIYFVQNHVKP 240

Query: 241 VAPSNSYQYSTSLLSDGFPVVQRPPNHHLLGYHPTNAKQVSGQTHLMSGTPNSNNNNNTN 300
           VAPSN+  Y +S L       Q+PP    L Y+ T              T N+ N N  N
Sbjct: 241 VAPSNNSSYLSSFLQK--QQQQQPPT---LDYYNT-------------ATVNATNLNALN 300

Query: 301 NN-GSSKSFVTILEEP-----------KTKLFGVAIQSKKRLHPEYGSNNIGKENNKARL 348
           ++  +S+S +T+LE+            KTKLFGV++ S K+      S++   + +K RL
Sbjct: 301 SSPPTSQSSITVLEDDHTNHHDQSNMRKTKLFGVSLPSSKK-----RSHHFSDQTSKTRL 346

BLAST of Tan0005604 vs. TAIR 10
Match: AT4G11660.1 (winged-helix DNA-binding transcription factor family protein )

HSP 1 Score: 191.8 bits (486), Expect = 9.8e-49
Identity = 103/214 (48.13%), Postives = 135/214 (63.08%), Query Frame = 0

Query: 16  DSHKAIPAPFLTKTYQLVDDPATDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSS 75
           DS ++IP PFLTKTYQLV+DP  D ++SW ED TTF+VWRP EFARDLLP YFKHNNFSS
Sbjct: 51  DSQRSIPTPFLTKTYQLVEDPVYDELISWNEDGTTFIVWRPAEFARDLLPKYFKHNNFSS 110

Query: 76  FVRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQLTVNQHHQSHSPVLG 135
           FVRQLNTYGFRK+VPDRWEF+N+ F++GEK LL +I RRK +QP +       + +    
Sbjct: 111 FVRQLNTYGFRKVVPDRWEFSNDCFKRGEKILLRDIQRRKISQPAMAAAAAAAAAAVAAS 170

Query: 136 VNNPSFYHFPARLSISPSDSDDNNPNWCDSPPLSSP-------GGVAAPNFNNSVTA--L 195
               +     A + +SPS+S +      +S P ++        GG +     +  TA  L
Sbjct: 171 AVTVAAVPVVAHI-VSPSNSGEEQVISSNSSPAAAAAAIGGVVGGGSLQRTTSCTTAPEL 230

Query: 196 SEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQN 221
            E+NERLR+ N  L  E+  +K LY +I   + N
Sbjct: 231 VEENERLRKDNERLRKEMTKLKGLYANIYTLMAN 263

BLAST of Tan0005604 vs. TAIR 10
Match: AT4G36990.1 (heat shock factor 4 )

HSP 1 Score: 186.0 bits (471), Expect = 5.4e-47
Identity = 101/208 (48.56%), Postives = 133/208 (63.94%), Query Frame = 0

Query: 17  SHKAIPAPFLTKTYQLVDDPATDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSF 76
           + +++PAPFL+KTYQLVDD +TD +VSW E+ T FVVW+  EFA+DLLP YFKHNNFSSF
Sbjct: 7   AQRSVPAPFLSKTYQLVDDHSTDDVVSWNEEGTAFVVWKTAEFAKDLLPQYFKHNNFSSF 66

Query: 77  VRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQLTVNQHHQSHSPVLGV 136
           +RQLNTYGFRK VPD+WEFAN++FR+G + LL +I RRK      +V         V+G 
Sbjct: 67  IRQLNTYGFRKTVPDKWEFANDYFRRGGEDLLTDIRRRK------SVIASTAGKCVVVG- 126

Query: 137 NNPSFYHFPARLSISPSDSDDNNPNWCDSPPLSSPGGVAAP-NFNNSVTALSEDNERLRR 196
                         SPS+S+    +   S   SSPG    P +  N V  LS +NE+L+R
Sbjct: 127 --------------SPSESNSGGGDDHGSSSTSSPGSSKNPGSVENMVADLSGENEKLKR 186

Query: 197 SNNMLMSELAHMKKLYNDIIYFVQNHVK 224
            NN L SELA  KK  ++++ F+  H+K
Sbjct: 187 ENNNLSSELAAAKKQRDELVTFLTGHLK 193

BLAST of Tan0005604 vs. TAIR 10
Match: AT5G62020.1 (heat shock transcription factor B2A )

HSP 1 Score: 178.7 bits (452), Expect = 8.6e-45
Identity = 99/226 (43.81%), Postives = 135/226 (59.73%), Query Frame = 0

Query: 17  SHKAIPAPFLTKTYQLVDDPATDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSF 76
           S ++IP PFLTKT+ LV+D + D ++SW ED ++F+VW P +FA+DLLP +FKHNNFSSF
Sbjct: 16  SQRSIPTPFLTKTFNLVEDSSIDDVISWNEDGSSFIVWNPTDFAKDLLPKHFKHNNFSSF 75

Query: 77  VRQLNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQLTVNQHHQSHSPVLGV 136
           VRQLNTYGF+K+VPDRWEF+N+FF++GEK LL EI RRK       +   HQ+      V
Sbjct: 76  VRQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQRRK-------ITTTHQT------V 135

Query: 137 NNPSFYHFPARLSISPSDS-DDNNPN----------WCDSPPLSSPGGVAAPNFNNSVTA 196
             PS       + +SPS+S +DNN N          +C     +  GG++          
Sbjct: 136 VAPSSEQRNQTMVVSPSNSGEDNNNNQVMSSSPSSWYCHQTKTTGNGGLS--------VE 195

Query: 197 LSEDNERLRRSNNMLMSELAHMKKLYNDIIYFVQNHVKPVAPSNSY 232
           L E+NE+LR  N  L  EL  MK + ++I   + N+V       SY
Sbjct: 196 LLEENEKLRSQNIQLNRELTQMKSICDNIYSLMSNYVGSQPTDRSY 220

BLAST of Tan0005604 vs. TAIR 10
Match: AT4G17750.1 (heat shock factor 1 )

HSP 1 Score: 161.0 bits (406), Expect = 1.9e-39
Identity = 72/110 (65.45%), Postives = 89/110 (80.91%), Query Frame = 0

Query: 20  AIPAPFLTKTYQLVDDPATDHIVSWGEDDTTFVVWRPPEFARDLLPNYFKHNNFSSFVRQ 79
           ++P PFL+KTY +V+DPATD IVSW   + +F+VW PPEF+RDLLP YFKHNNFSSFVRQ
Sbjct: 48  SLPPPFLSKTYDMVEDPATDAIVSWSPTNNSFIVWDPPEFSRDLLPKYFKHNNFSSFVRQ 107

Query: 80  LNTYGFRKIVPDRWEFANEFFRKGEKHLLCEIHRRKTAQPQLTVNQHHQS 130
           LNTYGFRK+ PDRWEFANE F +G+KHLL +I RRK+ Q   + + + QS
Sbjct: 108 LNTYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRKSVQGHGSSSSNPQS 157

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C6351.7e-9053.33Heat stress transcription factor B-4 OS=Arabidopsis thaliana OX=3702 GN=HSFB4 PE... [more]
Q7XHZ07.5e-7056.35Heat stress transcription factor B-4b OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q67U945.4e-6844.88Heat stress transcription factor B-4c OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q10KX81.6e-6755.29Heat stress transcription factor B-4d OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Q6Z9R82.2e-6151.13Putative heat stress transcription factor B-4a OS=Oryza sativa subsp. japonica O... [more]
Match NameE-valueIdentityDescription
XP_022940239.11.4e-17489.94heat stress transcription factor B-4-like [Cucurbita moschata] >XP_023525267.1 h... [more]
XP_022982267.11.8e-17489.94heat stress transcription factor B-4-like [Cucurbita maxima][more]
KAG6608467.11.8e-17489.43Heat stress transcription factor B-4, partial [Cucurbita argyrosperma subsp. sor... [more]
XP_038897232.18.9e-16184.57heat stress transcription factor B-4-like [Benincasa hispida][more]
XP_023536624.14.3e-15583.48heat stress transcription factor B-4-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1FJH26.9e-17589.94heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1IW739.0e-17589.94heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1F8Q81.8e-15483.19heat stress transcription factor B-4-like OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A6J1IFM14.3e-15381.87heat stress transcription factor B-4-like OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A0A0L4W81.6e-14779.21HSF_DOMAIN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G639930 ... [more]
Match NameE-valueIdentityDescription
AT1G46264.11.2e-9153.33heat shock transcription factor B4 [more]
AT4G11660.19.8e-4948.13winged-helix DNA-binding transcription factor family protein [more]
AT4G36990.15.4e-4748.56heat shock factor 4 [more]
AT5G62020.18.6e-4543.81heat shock transcription factor B2A [more]
AT4G17750.11.9e-3965.45heat shock factor 1 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 183..203
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 266..289
NoneNo IPR availablePANTHERPTHR10015:SF382BNAC06G00310D PROTEINcoord: 12..347
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 63..75
score: 78.68
coord: 25..48
score: 46.67
coord: 76..88
score: 84.62
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 21..114
e-value: 1.1E-59
score: 214.3
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 25..114
e-value: 3.1E-32
score: 111.1
IPR000232Heat shock factor (HSF)-type, DNA-bindingPROSITEPS00434HSF_DOMAINcoord: 64..88
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 17..116
e-value: 2.3E-37
score: 129.6
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 12..347
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 22..114

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0005604.2Tan0005604.2mRNA
Tan0005604.1Tan0005604.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0008356 asymmetric cell division
biological_process GO:0006357 regulation of transcription by RNA polymerase II
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function GO:0043565 sequence-specific DNA binding