Tan0005556 (gene) Snake gourd v1

BLAST of Tan0005556 vs. ExPASy Swiss-Prot
Match: O49675 (Probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=CCD4 PE=1 SV=1)

HSP 1 Score: 789.6 bits (2038), Expect = 2.4e-227
Identity = 383/586 (65.36%), Postives = 467/586 (79.69%), Query Frame = 0

Query: 23  SGPPVSTAISSVLTEENLKKTAPRPDVADSPSSSPSPSLPRRPPPPAIAKGPSTT----- 82
           S  P    I+S + EE             SP ++PS +  RR  P  +    + T     
Sbjct: 25  SSSPTLLRINSAVVEER------------SPITNPSDNNDRRNKPKTLHNRTNHTLVSSP 84

Query: 83  --RRLEPSLTARFFNAFDDLINNFINPPVTPSVDPRYVLANNFAPV-DELPPTDCEVIQG 142
              R E +L    F   +D+IN FI+PP  PSVDP++VL++NFAPV DELPPTDCE+I G
Sbjct: 85  PKLRPEMTLATALFTTVEDVINTFIDPPSRPSVDPKHVLSDNFAPVLDELPPTDCEIIHG 144

Query: 143 SLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLNSLRISNGRAVLCSRFVKTYKYTLER 202
           +LP SLNGAYIRNGPNPQ+LPRGPYHLFDGDGML++++I NG+A LCSR+VKTYKY +E+
Sbjct: 145 TLPLSLNGAYIRNGPNPQFLPRGPYHLFDGDGMLHAIKIHNGKATLCSRYVKTYKYNVEK 204

Query: 203 DAGYPLFPNVFSGFNGLTASAARGAVAAGRMLTGQYNPANGIGLANTSLAFFGDRLYALG 262
             G P+ PNVFSGFNG+TAS ARGA+ A R+LTGQYNP NGIGLANTSLAFF +RL+ALG
Sbjct: 205 QTGAPVMPNVFSGFNGVTASVARGALTAARVLTGQYNPVNGIGLANTSLAFFSNRLFALG 264

Query: 263 ESDLPYLIRLTPNGDIETLGRHDFDGKLTMSMTAHPKLDSDTGEAFAFRYGPLPPFLTFF 322
           ESDLPY +RLT +GDIET+GR+DFDGKL MSMTAHPK D  TGE FAFRYGP+PPFLT+F
Sbjct: 265 ESDLPYAVRLTESGDIETIGRYDFDGKLAMSMTAHPKTDPITGETFAFRYGPVPPFLTYF 324

Query: 323 RFDKNGAKQSDVPIFSMNRPTFLHDFAITKKYAVFTDIQIG--FNPMPMIIEGRSPVGSD 382
           RFD  G KQ DVPIFSM  P+FLHDFAITK++A+F +IQ+G   N + +++EG SPVG+D
Sbjct: 325 RFDSAGKKQRDVPIFSMTSPSFLHDFAITKRHAIFAEIQLGMRMNMLDLVLEGGSPVGTD 384

Query: 383 PSKVSRIGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDD--AVVLVAPNILSVEHTLE 442
             K  R+G+IP+YA DES+MKWF+VPG N+IHAINAWDEDD  +VVL+APNI+S+EHTLE
Sbjct: 385 NGKTPRLGVIPKYAGDESEMKWFEVPGFNIIHAINAWDEDDGNSVVLIAPNIMSIEHTLE 444

Query: 443 RTDLVHALMEKIRIDLKTGIVARRPLSTRNLDFGVLNPSYIGKKNRFVYAGVGDPMPKIS 502
           R DLVHAL+EK++IDL TGIV R P+S RNLDF V+NP+++G+ +R+VYA +GDPMPKIS
Sbjct: 445 RMDLVHALVEKVKIDLVTGIVRRHPISARNLDFAVINPAFLGRCSRYVYAAIGDPMPKIS 504

Query: 503 GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETAPEEDDGYVVSYVHDE 562
           GVVKLDVS+ +R DC VA R++G GCYGGEPFFV R+  + +    EEDDGYVV+YVHDE
Sbjct: 505 GVVKLDVSKGDRDDCTVARRMYGSGCYGGEPFFVARDPGNPE---AEEDDGYVVTYVHDE 564

Query: 563 NSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 597
            +GES+F+VMDAKSPELEI+AAV+LPRRVPYGFHGLFVKESDLNKL
Sbjct: 565 VTGESKFLVMDAKSPELEIVAAVRLPRRVPYGFHGLFVKESDLNKL 595

BLAST of Tan0005556 vs. ExPASy Swiss-Prot
Match: Q6YVJ0 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NCED1 PE=2 SV=1)

HSP 1 Score: 679.1 bits (1751), Expect = 4.5e-194
Identity = 341/604 (56.46%), Postives = 440/604 (72.85%), Query Frame = 0

Query: 12  RNLLLSPPIS------ISGPPVSTAISSVLTEEN------LKKTAPRPDVADSPSSSPSP 71
           +N   +PP +      ++ P V+T  +   TE+         KT        +  ++PS 
Sbjct: 42  KNASAAPPSAAASTTVLTSPLVTTTRTPKQTEQEDEQLVAKTKTTRTVIATTNGRAAPSQ 101

Query: 72  SLPRRPPPPAIAKGPSTTRRLEPSLTARFFNAFDDLINNFINPP-VTPSVDPRYVLANNF 131
           S PRR P PA A   +       SL   F NA +++IN FI+PP + P+VDPR VL +NF
Sbjct: 102 SRPRRRPAPAAAASAA-------SLPMTFCNALEEVINTFIDPPALRPAVDPRNVLTSNF 161

Query: 132 APVDELPPTDCEVIQGSLPSSL-NGAYIRNGPNPQYLPRGPYHLFDGDGMLNSLRI---- 191
            PVDELPPT C V++G++P  L  GAYIRNGPNPQ+LPRGP+HLFDGDGML+SL +    
Sbjct: 162 VPVDELPPTPCPVVRGAIPRCLAGGAYIRNGPNPQHLPRGPHHLFDGDGMLHSLLLPSPA 221

Query: 192 -SNGRAVLCSRFVKTYKYTLERDAGYPLFPNVFSGFNGLTASAARGAVAAGRMLTGQYNP 251
            S    VLCSR+V+TYKY +ERDAG P+ PNVFSGF+G+ A  ARGAV A R+LTGQ NP
Sbjct: 222 SSGDDPVLCSRYVQTYKYLVERDAGAPVLPNVFSGFHGV-AGMARGAVVAARVLTGQMNP 281

Query: 252 ANGIGLANTSLAFFGDRLYALGESDLPYLIRLTPN-GDIETLGRHDFDGKLTMSMTAHPK 311
             G+GLANTSLA+F  RLYALGESDLPY +R+ P+ G++ T GR DF G+L M MTAHPK
Sbjct: 282 LEGVGLANTSLAYFAGRLYALGESDLPYAVRVHPDTGEVTTHGRCDFGGRLVMGMTAHPK 341

Query: 312 LDSDTGEAFAFRYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRPTFLHDFAITKKYAVFTD 371
            D  TGE FAFRYGP+PPF+T+FRFD  G K +DVPIFS+ +P+FLHDFAIT++YA+F +
Sbjct: 342 KDPVTGELFAFRYGPVPPFVTYFRFDPAGNKGADVPIFSVQQPSFLHDFAITERYAIFPE 401

Query: 372 IQIGFNPMPMIIEGRSPVGSDPSKVSRIGLIPRYANDESKMKWFDVPGLNLIHAINAWDE 431
           IQI   PM M++ G SPVGSDP KV R+G+IPRYA DES+M+WF+VPG N++H++NAW+E
Sbjct: 402 IQIVMKPMDMVVGGGSPVGSDPGKVPRLGVIPRYATDESEMRWFEVPGFNIMHSVNAWEE 461

Query: 432 --DDAVVLVAPNILSVEHTLERTDLVHALMEKIRIDLKTGIVARRPLSTRNLDFGVLNPS 491
              + +VLVAPN+LS+EH LE  +LVH+ +EK+RI+L+TG+V R PL+  N DF V+NP+
Sbjct: 462 AGGEELVLVAPNVLSIEHALEHMELVHSCVEKVRINLRTGVVTRTPLAAGNFDFPVINPA 521

Query: 492 YIGKKNRFVYAGVGDPMPKISGVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERE 551
           ++G++NR+ Y GVGDP PKI GV KLD  +    DC VA R FGPGC+ GEPFFV  + E
Sbjct: 522 FLGRRNRYGYFGVGDPAPKIGGVAKLDFDRAGEGDCTVAQRDFGPGCFAGEPFFVADDVE 581

Query: 552 SSDETAPEEDDGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVK 594
            +      EDDGY+V YVHDE +GE+RF+VMDA+SP+LEI+A V+LP RVPYGFHGLFV 
Sbjct: 582 GNG----NEDDGYLVCYVHDEATGENRFVVMDARSPDLEIVAEVQLPGRVPYGFHGLFVT 633

BLAST of Tan0005556 vs. ExPASy Swiss-Prot
Match: Q5MBR6 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NCED2 PE=2 SV=1)

HSP 1 Score: 564.3 bits (1453), Expect = 1.6e-159
Identity = 283/523 (54.11%), Postives = 365/523 (69.79%), Query Frame = 0

Query: 83  SLTARFFNAFDDLINNFIN-PPVTPSVDPRYVLANNFAPVDELPPTDCEVIQGSLPSSL- 142
           SL     N  D +IN FI+ P   P+VDPR VL+ NFAPVDELPPT C VI+GS+PS L 
Sbjct: 55  SLPMAICNTVDKVINRFIDLPEQRPTVDPRRVLSGNFAPVDELPPTSCHVIRGSIPSCLA 114

Query: 143 NGAYIRNGPNPQY-LPRGPYHLFDGDGMLNSLRISNGRA------VLCSRFVKTYKYTLE 202
            G YIRNGPNPQ+ LP+  +HLFDGDGML+SL I +  +      VLCSR+V TYKY LE
Sbjct: 115 GGVYIRNGPNPQHRLPQRTHHLFDGDGMLHSLLIPSASSTLLSEPVLCSRYVHTYKYLLE 174

Query: 203 RDAGYPLFPNVFSGFNGLTASAARGAVAAGRMLTGQYNPANGIGLANTSLAFFGDRLYAL 262
           R+ G P+ PN F+GF+G+ A  AR  V   R+L GQ N   G GLANTS+  F D LYAL
Sbjct: 175 RETGGPVLPNFFAGFHGV-AGLARAVVMIARVLAGQINLNKGFGLANTSITLFADCLYAL 234

Query: 263 GESDLPYLIRLTP-NGDIETLGRHDFDGKLTMSMTAHPKLDSDTGEAFAFRYGPLPPFLT 322
            ESDLPY + + P NG++ TLGR DF G L+  MTAHPK D  T E FAFRY    PF+T
Sbjct: 235 CESDLPYSMHINPANGEVTTLGRCDFGGDLSFRMTAHPKKDPVTMELFAFRYNVFQPFIT 294

Query: 323 FFRFDKNGAKQSDVPIFSMNRPTFLHDFAITKKYAVFTDIQIGFNPMPMIIEGRSPVGSD 382
           +F FD+ G+K +DVPI S+ +P+ +HDFAIT++YA+F + Q+  NPM M++ G S VG D
Sbjct: 295 YFWFDRAGSKVADVPILSLQKPSVMHDFAITERYAIFPESQLIVNPMDMVMRGSSLVGLD 354

Query: 383 PSKVSRIGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDA--VVLVAPNILSVEHTLE 442
            + V RIG++PRYA DES M+WF+VP  N++H  N W+E D   +VLVAPNILS+EH L 
Sbjct: 355 RTMVPRIGVLPRYAKDESDMRWFEVPRFNMLHTTNGWEEADGEEIVLVAPNILSIEHMLG 414

Query: 443 RTDLVHALMEKIRIDLKTGIVARRPLSTRNLDFGVLNPSYIGKKNRFVYAGVGDPMPKIS 502
             +L+ A ++ +RI+L TG V+   LS  +L+FGV++  Y+G+KNR+ Y GV  P+PKI 
Sbjct: 415 NMELMRARVDMVRINLCTGDVSCTALSPESLEFGVIHQGYVGRKNRYGYFGVSGPLPKIK 474

Query: 503 GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETAPEEDDGYVVSYVHDE 562
           G+ KLD       DC V  R FG GC+ GEPFFVP     + +    ED GYVV Y H+E
Sbjct: 475 GIRKLDFDLVGSGDCTVGRRDFGLGCFAGEPFFVP----DNIDGYGNEDSGYVVCYTHEE 534

Query: 563 NSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDL 594
           ++GES F+VMDAKSPEL+I+A V+LP R+PYGFHG+FVK+++L
Sbjct: 535 DTGESWFVVMDAKSPELDIVAEVQLPSRIPYGFHGIFVKQAEL 572

BLAST of Tan0005556 vs. ExPASy Swiss-Prot
Match: Q84K96 (Zeaxanthin 7,8(7',8')-cleavage dioxygenase, chromoplastic OS=Crocus sativus OX=82528 GN=ZCD PE=1 SV=1)

HSP 1 Score: 437.2 bits (1123), Expect = 3.0e-121
Identity = 213/369 (57.72%), Postives = 273/369 (73.98%), Query Frame = 0

Query: 229 QYNPANGIGLANTSLAFFGDRLYALGESDLPYLIRLTP-NGDIETLGRHDFDGKLTMSMT 288
           Q +P  GIGLANTSL F   RL+AL E DLPY++RL+P +GDI T+GR + +   T S T
Sbjct: 2   QVDPTKGIGLANTSLQFSNGRLHALCEYDLPYVVRLSPEDGDISTVGRIE-NNVSTKSTT 61

Query: 289 AHPKLDSDTGEAFAFRYGPLPPFLTFFRFDKNGAKQS-DVPIFSMNRPTFLHDFAITKKY 348
           AHPK D  TGE F+F YGP+ P++T+ R+D +G K   DVPIFS   P+F+HDFAIT+ Y
Sbjct: 62  AHPKTDPVTGETFSFSYGPIQPYVTYSRYDCDGKKSGPDVPIFSFKEPSFVHDFAITEHY 121

Query: 349 AVFTDIQIGFNPMPMIIEGRSPVGSDPSKVSRIGLIPRYANDESKMKWFDVPGLNLIHAI 408
           AVF DIQI   P   I+ GR  +G D  KV R+GL+PRYA  +S+M+WFDVPG N++H +
Sbjct: 122 AVFPDIQIVMKPAE-IVRGRRMIGPDLEKVPRLGLLPRYATSDSEMRWFDVPGFNMVHVV 181

Query: 409 NAWDED--DAVVLVAPNILSVEHTLERTDLVHALMEKIRIDLKTGIVARRPLSTRNLDFG 468
           NAW+E+  + VV+VAPN+  +E+ ++R DL+H  +E  RI+LK+G V+R  LS  NLDFG
Sbjct: 182 NAWEEEGGEVVVIVAPNVSPIENAIDRFDLLHVSVEMARIELKSGSVSRTLLSAENLDFG 241

Query: 469 VLNPSYIGKKNRFVYAGVGDPMPKISGVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFV 528
           V++  Y G+K+R+ Y GVGDPMPKI GVVK+D     R +C+VA R FG GC+GGEPFFV
Sbjct: 242 VIHRGYSGRKSRYAYLGVGDPMPKIRGVVKVDFELAGRGECVVARREFGVGCFGGEPFFV 301

Query: 529 PRERESSDETAPEEDDGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFH 588
           P    SS ++  EEDDGYVVSY+HDE  GES F+VMDA+SPELEI+A V LPRRVPYGFH
Sbjct: 302 P---ASSKKSGGEEDDGYVVSYLHDEGKGESSFVVMDARSPELEILAEVVLPRRVPYGFH 361

Query: 589 GLFVKESDL 594
           GLFV E++L
Sbjct: 362 GLFVTEAEL 365

BLAST of Tan0005556 vs. ExPASy Swiss-Prot
Match: Q9C6Z1 (Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED5 PE=1 SV=1)

HSP 1 Score: 395.6 bits (1015), Expect = 1.0e-108
Identity = 226/583 (38.77%), Postives = 333/583 (57.12%), Query Frame = 0

Query: 19  PISISGPPVSTAISSVLTEENLKKTAPRPDVADSPSSSPSPSLPRRPPPPAIAKGPSTTR 78
           P  +  P  S+++S   T+   +K +    V+D+P+    P+ P  P P    K  S   
Sbjct: 20  PSDLDAPSPSSSVSFTNTKPRRRKLSAN-SVSDTPNLLNFPNYP-SPNPIIPEKDTSRWN 79

Query: 79  RLEPSLTARFFNAFDDLINNFINPPVTPSVDPRYVLANNFAPVDELPPTDCEVIQGSLPS 138
            L+ + +A    A   L+    + P+  +VDPR+ ++ N+APV E        + G +P 
Sbjct: 80  PLQRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSVDGKIPD 139

Query: 139 SLNGAYIRNGPNPQYLPRGPYHLFDGDGMLNSLRISNGRAVLCSRFVKTYKYTLERDAGY 198
            ++G Y+RNG NP + P   +HLFDGDGM+++++I+NG A    RF +T +   E+  G 
Sbjct: 140 CIDGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKITNGDASYSCRFTETERLVQEKQLGS 199

Query: 199 PLFPNVFSGFNGLTASAARGAVAAGRMLTGQYNPANGIGLANTSLAFFGDRLYALGESDL 258
           P+FP      +G  +  AR  +   R L G  N  NG G+AN  L +F DRL A+ E DL
Sbjct: 200 PIFPKAIGELHG-HSGIARLMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDL 259

Query: 259 PYLIRLTPNGDIETLGRHDFDGKLTMSMTAHPKLDSDTGEAFAFRYGPL-PPFLTFFRFD 318
           PY +R+T NGD+ET+GR DFDG+L+ +M AHPK+D  T E FA  Y  +  P+L +F+F 
Sbjct: 260 PYQVRVTDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLKYFKFS 319

Query: 319 KNGAKQSDVPIFSMNRPTFLHDFAITKKYAVFTDIQIGFNPMPMIIEGRSPVGSDPSKVS 378
             G K  DV I  +  PT +HDFAIT+ + V  D Q+ F    M + G+SPV  D  K+S
Sbjct: 320 PEGEKSPDVEI-PLASPTMMHDFAITENFVVIPDQQVVFKLSDMFL-GKSPVKYDGEKIS 379

Query: 379 RIGLIPRYANDESKMKWFDVPGLNLIHAINAWD--EDDAVVLVAPNILSVEHTLERTD-L 438
           R G++PR A D S+M W + P     H  NAW+  E D VV++   +   +      D  
Sbjct: 380 RFGILPRNAKDASEMVWVESPETFCFHLWNAWESPETDEVVVIGSCMTPADSIFNECDEQ 439

Query: 439 VHALMEKIRIDLKTGIVARRPL----STRNLDFGVLNPSYIGKKNRFVYAGVGDPMPKIS 498
           +++++ +IR++LKTG   RR +       NL+ G++N + +G+K R+ Y  + +P PK+S
Sbjct: 440 LNSVLSEIRLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGRKTRYAYLAIAEPWPKVS 499

Query: 499 GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETAPEEDDGYVVSYVHDE 558
           G  K+D+S  E     V +  +G   YGGEPFF+PR  ES       EDDGY++S+VHDE
Sbjct: 500 GFAKVDLSTGE-----VKNHFYGGKKYGGEPFFLPRGLESDG-----EDDGYIMSFVHDE 559

Query: 559 NSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDL 594
            S ES   +++A + ELE  A VKLP RVPYGFHG FV  +D+
Sbjct: 560 ESWESELHIVNAVTLELE--ATVKLPSRVPYGFHGTFVNSADM 585

BLAST of Tan0005556 vs. NCBI nr
Match: XP_038897022.1 (probable carotenoid cleavage dioxygenase 4, chloroplastic [Benincasa hispida])

HSP 1 Score: 1092.4 bits (2824), Expect = 0.0e+00
Identity = 540/594 (90.91%), Postives = 567/594 (95.45%), Query Frame = 0

Query: 1   MGAISSPFFSGRNLLLSPPISISGPPVSTAISSVLTEENLKKTAPRPDVADSPSSSPSPS 60
           M AISS F SG NLLLSPPISIS PP+STAI SVLTEE +KKTAPRPD     + SPSPS
Sbjct: 1   MDAISSSFLSGGNLLLSPPISISRPPISTAIYSVLTEETVKKTAPRPD-----ADSPSPS 60

Query: 61  LPRR-PPPPAIAKGPSTTRRLEPSLTARFFNAFDDLINNFINPPVTPSVDPRYVLANNFA 120
           LPRR  PPPAIAKG S+TRR+EPSL ARFFNAFDDLINNFINPPV+PSVDPRYVLA+NFA
Sbjct: 61  LPRRASPPPAIAKG-SSTRRIEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFA 120

Query: 121 PVDELPPTDCEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLNSLRISNGRAV 180
           PVDELPPT+CEVI GSLP SLNGAYIRNGPNPQYLPRGPYHLFDGDGML+SLRISNGRAV
Sbjct: 121 PVDELPPTECEVIHGSLPPSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAV 180

Query: 181 LCSRFVKTYKYTLERDAGYPLFPNVFSGFNGLTASAARGAVAAGRMLTGQYNPANGIGLA 240
           LCSR+VKTYKYTLERDAG+P+FPNVFSGFNGLTASAARGAVAAGR+LTGQ+NPANGIGLA
Sbjct: 181 LCSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQFNPANGIGLA 240

Query: 241 NTSLAFFGDRLYALGESDLPYLIRLTPNGDIETLGRHDFDGKLTMSMTAHPKLDSDTGEA 300
           NTSLAFFGDRLYALGESDLPY IRLTPNGDIETLGRHDFDGKLTMSMTAHPK+DSDTGEA
Sbjct: 241 NTSLAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLTMSMTAHPKVDSDTGEA 300

Query: 301 FAFRYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRPTFLHDFAITKKYAVFTDIQIGFNPM 360
           FAFRYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRP+FLHDFAITKKYAVFT+IQIGFNPM
Sbjct: 301 FAFRYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPM 360

Query: 361 PMIIEGRSPVGSDPSKVSRIGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVLVA 420
            M IEGRSPVGSDPSK+SR+GLIPRYANDESKMKWFDVPGLNL+HAINAWDEDDAVVL+A
Sbjct: 361 QM-IEGRSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLVHAINAWDEDDAVVLIA 420

Query: 421 PNILSVEHTLERTDLVHALMEKIRIDLKTGIVARRPLSTRNLDFGVLNPSYIGKKNRFVY 480
           PNILSVEHTLER DLVHAL+EK+RIDLKTGIV RRPLSTRNLDFGV++PSY+GKKNRFVY
Sbjct: 421 PNILSVEHTLERMDLVHALVEKVRIDLKTGIVTRRPLSTRNLDFGVIHPSYVGKKNRFVY 480

Query: 481 AGVGDPMPKISGVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETAPEED 540
           AGVGDPMPKISGVVKLDVSQEERRDCIVA RIFGPGCYGGEPFFVPRERESS+ET  EED
Sbjct: 481 AGVGDPMPKISGVVKLDVSQEERRDCIVACRIFGPGCYGGEPFFVPRERESSEETPAEED 540

Query: 541 DGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDL 594
           DGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDL
Sbjct: 541 DGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDL 587

BLAST of Tan0005556 vs. NCBI nr
Match: KAG6600436.1 (putative carotenoid cleavage dioxygenase 4, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1063.9 bits (2750), Expect = 5.0e-307
Identity = 528/596 (88.59%), Postives = 556/596 (93.29%), Query Frame = 0

Query: 1   MGAISSPFFSGRNLLLSPPISISGPPVSTAISSVLTEENLKKTAPRPDVADSPSSSPSPS 60
           M A+SSPF +G  LLLSPPIS S PP S AI+SV TEE   KT  R + ADSPS      
Sbjct: 1   MDALSSPFLTGGKLLLSPPISTSRPPFSMAITSVFTEET-PKTVKRTN-ADSPS------ 60

Query: 61  LPRRPPPPAIAKGPSTTRRLEPSLTARFFNAFDDLINNFINPPVTPSVDPRYVLANNFAP 120
            PRRPPPPA+AKGPSTT RLEPSL ARFFNAFDDLINNFINPPV+PSVDPRYVLA+NFAP
Sbjct: 61  -PRRPPPPAMAKGPSTT-RLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAP 120

Query: 121 VDELPPTDCEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLNSLRISNGRAVL 180
           VDELPPT+CEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGML+SLRISNGRAVL
Sbjct: 121 VDELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVL 180

Query: 181 CSRFVKTYKYTLERDAGYPLFPNVFSGFNGLTASAARGAVAAGRMLTGQYNPANGIGLAN 240
           CSR+VKTYKYTLERDAG+P+FPNVFSGFNGLTASAARGAVAAGR+LTGQYNPANGIGLAN
Sbjct: 181 CSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLAN 240

Query: 241 TSLAFFGDRLYALGESDLPYLIRLTPNGDIETLGRHDFDGKLTMSMTAHPKLDSDTGEAF 300
           TS+AFFGDRLYALGESDLPY IRL PNG+IETLGR DFDG L MSMTAHPK D DTGE F
Sbjct: 241 TSVAFFGDRLYALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKSDPDTGETF 300

Query: 301 AFRYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRPTFLHDFAITKKYAVFTDIQIGFNPMP 360
           AFRYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRP+FLHDFAIT+KYAVFT+IQIGFNPM 
Sbjct: 301 AFRYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITQKYAVFTEIQIGFNPML 360

Query: 361 MIIEGRSPVGSDPSKVSRIGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVLVAP 420
           MI+EGRSPVG+DPS VSR+GLIPRYANDESKMKWFDVPG NLIHAINAWDEDDAVVL+AP
Sbjct: 361 MIMEGRSPVGTDPSTVSRVGLIPRYANDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAP 420

Query: 421 NILSVEHTLERTDLVHALMEKIRIDLKTGIVARRPLSTRNLDFGVLNPSYIGKKNRFVYA 480
           NILSVEHTLER DLVHAL+E++RIDLKTGIV RRPLSTRNLDFGV+NPSYIGKKNRFVYA
Sbjct: 421 NILSVEHTLERMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFVYA 480

Query: 481 GVGDPMPKISGVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETAPEEDD 540
           GVGDPMPKISGVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETA EEDD
Sbjct: 481 GVGDPMPKISGVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDD 540

Query: 541 GYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 597
           GYVVSYVHDENSGES+FIVMDAKSP+LEIIA VKLPRRVPYGFHGLFVKESDLNKL
Sbjct: 541 GYVVSYVHDENSGESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKESDLNKL 586

BLAST of Tan0005556 vs. NCBI nr
Match: XP_004147771.1 (probable carotenoid cleavage dioxygenase 4, chloroplastic [Cucumis sativus] >KGN53473.1 hypothetical protein Csa_014438 [Cucumis sativus])

HSP 1 Score: 1063.5 bits (2749), Expect = 6.5e-307
Identity = 520/596 (87.25%), Postives = 553/596 (92.79%), Query Frame = 0

Query: 1   MGAISSPFFSGRNLLLSPPISISGPPVSTAISSVLTEENLKKTAPRPDVADSPSSSPSPS 60
           M +ISSPF SGRNL+LSPPIS S PP+ST I SVLTE+N+KK  P PD     + SPSP 
Sbjct: 1   MDSISSPFLSGRNLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPD-----ADSPSPP 60

Query: 61  LPRRPPPPAIAKGPSTTRRLEPSLTARFFNAFDDLINNFINPPVTPSVDPRYVLANNFAP 120
           LPR  PP       S+TRR++PSL ARFFNAFDDLINNFINPPV+PSVDPRY+LA+NFAP
Sbjct: 61  LPRPSPPSPPMPRVSSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAP 120

Query: 121 VDELPPTDCEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLNSLRISNGRAVL 180
           VDELPPT+CEVI GSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGML+SLRIS+GRAVL
Sbjct: 121 VDELPPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVL 180

Query: 181 CSRFVKTYKYTLERDAGYPLFPNVFSGFNGLTASAARGAVAAGRMLTGQYNPANGIGLAN 240
           CSR+VKTYKYTLERDAG+P+FPNVFSGFNGLTASAARGAVA GR+LTGQYNPANGIGLAN
Sbjct: 181 CSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLAN 240

Query: 241 TSLAFFGDRLYALGESDLPYLIRLTPNGDIETLGRHDFDGKLTMSMTAHPKLDSDTGEAF 300
           TSLAFFGDRLYALGESDLPY IRLTPNGDIETL RHDFDGKLT+SMTAHPK+DSDTGEAF
Sbjct: 241 TSLAFFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAF 300

Query: 301 AFRYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRPTFLHDFAITKKYAVFTDIQIGFNPMP 360
           AFRYGPLPPFLT+FRFDKNGAK SDVPI SMNRP+FLHDFAITKKYAVFTDIQIG NP  
Sbjct: 301 AFRYGPLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQ 360

Query: 361 MIIEGRSPVGSDPSKVSRIGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVLVAP 420
           MIIEG SPVGSDPSK+SR+GLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVV+VAP
Sbjct: 361 MIIEGGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVAP 420

Query: 421 NILSVEHTLERTDLVHALMEKIRIDLKTGIVARRPLSTRNLDFGVLNPSYIGKKNRFVYA 480
           NILSVEH LER DLVHAL+EKIRIDLKTGIV R PLSTRNLDFGV++PSY+GKK+RFVYA
Sbjct: 421 NILSVEHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYA 480

Query: 481 GVGDPMPKISGVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETAPEEDD 540
           GVGDPMPKISGVVKL++SQEERRDCIVA RIFGPGCYGGEPFFVPRERESSDET  EEDD
Sbjct: 481 GVGDPMPKISGVVKLEISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDETEAEEDD 540

Query: 541 GYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 597
           GYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL
Sbjct: 541 GYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 591

BLAST of Tan0005556 vs. NCBI nr
Match: XP_023531023.1 (probable carotenoid cleavage dioxygenase 4, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1061.6 bits (2744), Expect = 2.5e-306
Identity = 525/596 (88.09%), Postives = 556/596 (93.29%), Query Frame = 0

Query: 1   MGAISSPFFSGRNLLLSPPISISGPPVSTAISSVLTEENLKKTAPRPDVADSPSSSPSPS 60
           M AI SPF +G NLLLSPPIS + PP S AI+SV TEE   KT  + + ADSPS      
Sbjct: 1   MDAIFSPFLTGGNLLLSPPISTARPPFSVAITSVFTEET-PKTVKKTN-ADSPS------ 60

Query: 61  LPRRPPPPAIAKGPSTTRRLEPSLTARFFNAFDDLINNFINPPVTPSVDPRYVLANNFAP 120
            PRRPPPPA+AKGPSTT RLEPSL ARFFNAFDDLINNFINPPV+PSVDPRYVLA+NFAP
Sbjct: 61  -PRRPPPPAMAKGPSTT-RLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAP 120

Query: 121 VDELPPTDCEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLNSLRISNGRAVL 180
           VDELPPT+CEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGML+SLRISNGRAVL
Sbjct: 121 VDELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVL 180

Query: 181 CSRFVKTYKYTLERDAGYPLFPNVFSGFNGLTASAARGAVAAGRMLTGQYNPANGIGLAN 240
           CSR+VKTYKYTLERDAG+P+FPNVFSGFNGLTASAARGAVAAGR+LTGQYNPANGIGLAN
Sbjct: 181 CSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLAN 240

Query: 241 TSLAFFGDRLYALGESDLPYLIRLTPNGDIETLGRHDFDGKLTMSMTAHPKLDSDTGEAF 300
           TS+AFFGDRLYALGESDLPY IRL PNG+IETLGR DFDG L MSMTAHPK D DTGE F
Sbjct: 241 TSVAFFGDRLYALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKSDPDTGETF 300

Query: 301 AFRYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRPTFLHDFAITKKYAVFTDIQIGFNPMP 360
           AFRYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRP+FLHDFAITKKYAVFT+IQIGFNPM 
Sbjct: 301 AFRYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPML 360

Query: 361 MIIEGRSPVGSDPSKVSRIGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVLVAP 420
           MI+EGRSPVG+DPS VSR+GLIPRYANDESKMKWFDVPG NLIHAINAWDEDDAVVL+AP
Sbjct: 361 MIMEGRSPVGTDPSTVSRVGLIPRYANDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAP 420

Query: 421 NILSVEHTLERTDLVHALMEKIRIDLKTGIVARRPLSTRNLDFGVLNPSYIGKKNRFVYA 480
           NILSVEHTLER DLVHAL+E++RIDLKTGIV RRPLSTRNLDFGV+NPSYIGKKNRF+YA
Sbjct: 421 NILSVEHTLERMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFIYA 480

Query: 481 GVGDPMPKISGVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETAPEEDD 540
           GVGDPMPKISGVVKLDVSQ+ERRDCIVASRIFGPGCYGGEPFFVPRERESSDETA EEDD
Sbjct: 481 GVGDPMPKISGVVKLDVSQQERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDD 540

Query: 541 GYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 597
           GYVVSYVHDENSGES+FIVMDAKSP+LEIIA VKLPRRVPYGFHGLFVKE+DLNKL
Sbjct: 541 GYVVSYVHDENSGESKFIVMDAKSPKLEIIAVVKLPRRVPYGFHGLFVKETDLNKL 586

BLAST of Tan0005556 vs. NCBI nr
Match: AXM43896.1 (carotenoid cleavage dioxygenase [Cucurbita pepo])

HSP 1 Score: 1060.4 bits (2741), Expect = 5.5e-306
Identity = 523/600 (87.17%), Postives = 560/600 (93.33%), Query Frame = 0

Query: 1   MGAISSPFFSGRNLLLSPPISISGPPVSTAISSVLTEE---NLKKTAPRPDVADSPSSSP 60
           M AISSPF SG NLLLS PIS S   VSTAI SVL EE    +KK A RPD     +  P
Sbjct: 1   MDAISSPFLSGGNLLLSTPISKSRTAVSTAICSVLAEETPRTVKKPASRPD-----ADFP 60

Query: 61  SPSLPRR-PPPPAIAKGPSTTRRLEPSLTARFFNAFDDLINNFINPPVTPSVDPRYVLAN 120
           SPSL +R  PPPA+AKG S+TRR+EPS+ A  FNAFDDLINNFINPPV+PSVDPRYVLA+
Sbjct: 61  SPSLSQRATPPPAVAKG-SSTRRVEPSVPASIFNAFDDLINNFINPPVSPSVDPRYVLAD 120

Query: 121 NFAPVDELPPTDCEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLNSLRISNG 180
           NFAPVDELPPT+CEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGML+SLRISNG
Sbjct: 121 NFAPVDELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNG 180

Query: 181 RAVLCSRFVKTYKYTLERDAGYPLFPNVFSGFNGLTASAARGAVAAGRMLTGQYNPANGI 240
           RAVLCSR+VKTYKYTLERDAG+P+FPNVFSGFNGLTASAARGAVAAGR+LTGQYNPANGI
Sbjct: 181 RAVLCSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGI 240

Query: 241 GLANTSLAFFGDRLYALGESDLPYLIRLTPNGDIETLGRHDFDGKLTMSMTAHPKLDSDT 300
           GLANTS+AFFGDRLYALGESDLPY IRLTPNGDIETLGRHDFDGKL+MSMTAHPK+D DT
Sbjct: 241 GLANTSVAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDT 300

Query: 301 GEAFAFRYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRPTFLHDFAITKKYAVFTDIQIGF 360
           GEAFAFRYGPLPPFLTFFRFDKNGAKQ DVPIFSMNRP+FLHDFAITKKYAVFT+IQIGF
Sbjct: 301 GEAFAFRYGPLPPFLTFFRFDKNGAKQPDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGF 360

Query: 361 NPMPMIIEGRSPVGSDPSKVSRIGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVV 420
           NPM MI+EGRSPVG+DPSK+SRIGLIPRYANDESKMKWFDVPG+NL+HAINAWDEDDAV+
Sbjct: 361 NPMQMIVEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVI 420

Query: 421 LVAPNILSVEHTLERTDLVHALMEKIRIDLKTGIVARRPLSTRNLDFGVLNPSYIGKKNR 480
           LVAPNILSVEHT+ER DLVHAL+EKIRIDLKTGI+ RRPLSTRNLDFGV+NP Y+GKKNR
Sbjct: 421 LVAPNILSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLYVGKKNR 480

Query: 481 FVYAGVGDPMPKISGVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETAP 540
           FVYAGVGDPMPKISGVVKLDVSQEERRDCIVA+R+FGP CYGGEPFFVPRERE+SDETA 
Sbjct: 481 FVYAGVGDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDETAA 540

Query: 541 EEDDGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 597
           EEDDGYVVSYVHDENSG+SRF+VMDAKSPELEIIA VKLPRRVPYGFHGLFVKESDL+KL
Sbjct: 541 EEDDGYVVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 594

BLAST of Tan0005556 vs. ExPASy TrEMBL
Match: A0A0A0KV99 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G056640 PE=3 SV=1)

HSP 1 Score: 1063.5 bits (2749), Expect = 3.2e-307
Identity = 520/596 (87.25%), Postives = 553/596 (92.79%), Query Frame = 0

Query: 1   MGAISSPFFSGRNLLLSPPISISGPPVSTAISSVLTEENLKKTAPRPDVADSPSSSPSPS 60
           M +ISSPF SGRNL+LSPPIS S PP+ST I SVLTE+N+KK  P PD     + SPSP 
Sbjct: 1   MDSISSPFLSGRNLILSPPISSSLPPISTPIYSVLTEQNVKKNTPPPD-----ADSPSPP 60

Query: 61  LPRRPPPPAIAKGPSTTRRLEPSLTARFFNAFDDLINNFINPPVTPSVDPRYVLANNFAP 120
           LPR  PP       S+TRR++PSL ARFFNAFDDLINNFINPPV+PSVDPRY+LA+NFAP
Sbjct: 61  LPRPSPPSPPMPRVSSTRRVQPSLPARFFNAFDDLINNFINPPVSPSVDPRYILADNFAP 120

Query: 121 VDELPPTDCEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLNSLRISNGRAVL 180
           VDELPPT+CEVI GSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGML+SLRIS+GRAVL
Sbjct: 121 VDELPPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISDGRAVL 180

Query: 181 CSRFVKTYKYTLERDAGYPLFPNVFSGFNGLTASAARGAVAAGRMLTGQYNPANGIGLAN 240
           CSR+VKTYKYTLERDAG+P+FPNVFSGFNGLTASAARGAVA GR+LTGQYNPANGIGLAN
Sbjct: 181 CSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAVGRILTGQYNPANGIGLAN 240

Query: 241 TSLAFFGDRLYALGESDLPYLIRLTPNGDIETLGRHDFDGKLTMSMTAHPKLDSDTGEAF 300
           TSLAFFGDRLYALGESDLPY IRLTPNGDIETL RHDFDGKLT+SMTAHPK+DSDTGEAF
Sbjct: 241 TSLAFFGDRLYALGESDLPYPIRLTPNGDIETLARHDFDGKLTLSMTAHPKVDSDTGEAF 300

Query: 301 AFRYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRPTFLHDFAITKKYAVFTDIQIGFNPMP 360
           AFRYGPLPPFLT+FRFDKNGAK SDVPI SMNRP+FLHDFAITKKYAVFTDIQIG NP  
Sbjct: 301 AFRYGPLPPFLTYFRFDKNGAKHSDVPILSMNRPSFLHDFAITKKYAVFTDIQIGINPTQ 360

Query: 361 MIIEGRSPVGSDPSKVSRIGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVLVAP 420
           MIIEG SPVGSDPSK+SR+GLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVV+VAP
Sbjct: 361 MIIEGGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVIVAP 420

Query: 421 NILSVEHTLERTDLVHALMEKIRIDLKTGIVARRPLSTRNLDFGVLNPSYIGKKNRFVYA 480
           NILSVEH LER DLVHAL+EKIRIDLKTGIV R PLSTRNLDFGV++PSY+GKK+RFVYA
Sbjct: 421 NILSVEHALERMDLVHALVEKIRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKHRFVYA 480

Query: 481 GVGDPMPKISGVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETAPEEDD 540
           GVGDPMPKISGVVKL++SQEERRDCIVA RIFGPGCYGGEPFFVPRERESSDET  EEDD
Sbjct: 481 GVGDPMPKISGVVKLEISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDETEAEEDD 540

Query: 541 GYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 597
           GYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL
Sbjct: 541 GYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 591

BLAST of Tan0005556 vs. ExPASy TrEMBL
Match: A0A346DA05 (Carotenoid cleavage dioxygenase OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 1060.4 bits (2741), Expect = 2.7e-306
Identity = 523/600 (87.17%), Postives = 560/600 (93.33%), Query Frame = 0

Query: 1   MGAISSPFFSGRNLLLSPPISISGPPVSTAISSVLTEE---NLKKTAPRPDVADSPSSSP 60
           M AISSPF SG NLLLS PIS S   VSTAI SVL EE    +KK A RPD     +  P
Sbjct: 1   MDAISSPFLSGGNLLLSTPISKSRTAVSTAICSVLAEETPRTVKKPASRPD-----ADFP 60

Query: 61  SPSLPRR-PPPPAIAKGPSTTRRLEPSLTARFFNAFDDLINNFINPPVTPSVDPRYVLAN 120
           SPSL +R  PPPA+AKG S+TRR+EPS+ A  FNAFDDLINNFINPPV+PSVDPRYVLA+
Sbjct: 61  SPSLSQRATPPPAVAKG-SSTRRVEPSVPASIFNAFDDLINNFINPPVSPSVDPRYVLAD 120

Query: 121 NFAPVDELPPTDCEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLNSLRISNG 180
           NFAPVDELPPT+CEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGML+SLRISNG
Sbjct: 121 NFAPVDELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNG 180

Query: 181 RAVLCSRFVKTYKYTLERDAGYPLFPNVFSGFNGLTASAARGAVAAGRMLTGQYNPANGI 240
           RAVLCSR+VKTYKYTLERDAG+P+FPNVFSGFNGLTASAARGAVAAGR+LTGQYNPANGI
Sbjct: 181 RAVLCSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGI 240

Query: 241 GLANTSLAFFGDRLYALGESDLPYLIRLTPNGDIETLGRHDFDGKLTMSMTAHPKLDSDT 300
           GLANTS+AFFGDRLYALGESDLPY IRLTPNGDIETLGRHDFDGKL+MSMTAHPK+D DT
Sbjct: 241 GLANTSVAFFGDRLYALGESDLPYPIRLTPNGDIETLGRHDFDGKLSMSMTAHPKIDYDT 300

Query: 301 GEAFAFRYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRPTFLHDFAITKKYAVFTDIQIGF 360
           GEAFAFRYGPLPPFLTFFRFDKNGAKQ DVPIFSMNRP+FLHDFAITKKYAVFT+IQIGF
Sbjct: 301 GEAFAFRYGPLPPFLTFFRFDKNGAKQPDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGF 360

Query: 361 NPMPMIIEGRSPVGSDPSKVSRIGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVV 420
           NPM MI+EGRSPVG+DPSK+SRIGLIPRYANDESKMKWFDVPG+NL+HAINAWDEDDAV+
Sbjct: 361 NPMQMIVEGRSPVGADPSKISRIGLIPRYANDESKMKWFDVPGVNLVHAINAWDEDDAVI 420

Query: 421 LVAPNILSVEHTLERTDLVHALMEKIRIDLKTGIVARRPLSTRNLDFGVLNPSYIGKKNR 480
           LVAPNILSVEHT+ER DLVHAL+EKIRIDLKTGI+ RRPLSTRNLDFGV+NP Y+GKKNR
Sbjct: 421 LVAPNILSVEHTMERMDLVHALVEKIRIDLKTGIITRRPLSTRNLDFGVINPLYVGKKNR 480

Query: 481 FVYAGVGDPMPKISGVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETAP 540
           FVYAGVGDPMPKISGVVKLDVSQEERRDCIVA+R+FGP CYGGEPFFVPRERE+SDETA 
Sbjct: 481 FVYAGVGDPMPKISGVVKLDVSQEERRDCIVATRLFGPNCYGGEPFFVPRERENSDETAA 540

Query: 541 EEDDGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 597
           EEDDGYVVSYVHDENSG+SRF+VMDAKSPELEIIA VKLPRRVPYGFHGLFVKESDL+KL
Sbjct: 541 EEDDGYVVSYVHDENSGDSRFVVMDAKSPELEIIATVKLPRRVPYGFHGLFVKESDLSKL 594

BLAST of Tan0005556 vs. ExPASy TrEMBL
Match: A0A6J1FTM6 (probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111448011 PE=3 SV=1)

HSP 1 Score: 1058.9 bits (2737), Expect = 7.8e-306
Identity = 526/596 (88.26%), Postives = 554/596 (92.95%), Query Frame = 0

Query: 1   MGAISSPFFSGRNLLLSPPISISGPPVSTAISSVLTEENLKKTAPRPDVADSPSSSPSPS 60
           M A+SSPF +G NLLLSPPIS S PP S AI+SV TEE   KT  R + ADSPS      
Sbjct: 1   MDALSSPFLTGGNLLLSPPISTSRPPFSMAITSVFTEET-PKTVKRTN-ADSPS------ 60

Query: 61  LPRRPPPPAIAKGPSTTRRLEPSLTARFFNAFDDLINNFINPPVTPSVDPRYVLANNFAP 120
            PRRPPPPA+AKGPSTT RLEPSL ARFFNAFDDLINNFINPPV+PSVDPRYVLA+NFAP
Sbjct: 61  -PRRPPPPAMAKGPSTT-RLEPSLPARFFNAFDDLINNFINPPVSPSVDPRYVLADNFAP 120

Query: 121 VDELPPTDCEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLNSLRISNGRAVL 180
           VDELPPT+CEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGML+SLRISNGRAVL
Sbjct: 121 VDELPPTECEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAVL 180

Query: 181 CSRFVKTYKYTLERDAGYPLFPNVFSGFNGLTASAARGAVAAGRMLTGQYNPANGIGLAN 240
           CSR+VKTYKYTLERDAG+P+FPNVFSGFNGLTASAARGAVAAGR+LTGQYNPANGIGLAN
Sbjct: 181 CSRYVKTYKYTLERDAGHPVFPNVFSGFNGLTASAARGAVAAGRVLTGQYNPANGIGLAN 240

Query: 241 TSLAFFGDRLYALGESDLPYLIRLTPNGDIETLGRHDFDGKLTMSMTAHPKLDSDTGEAF 300
           TS+AFFGD LYALGESDLPY IRL PNG+IETLGR DFDG L MSMTAHPK D DTGE F
Sbjct: 241 TSVAFFGDSLYALGESDLPYSIRLAPNGEIETLGRQDFDGNLFMSMTAHPKFDPDTGETF 300

Query: 301 AFRYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRPTFLHDFAITKKYAVFTDIQIGFNPMP 360
           AFRYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRP+FLHDFAITKKYAVFT+IQIGFNPM 
Sbjct: 301 AFRYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRPSFLHDFAITKKYAVFTEIQIGFNPML 360

Query: 361 MIIEGRSPVGSDPSKVSRIGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVLVAP 420
           MI+EGRSPVG+DPS VSR+GLIPRYA+DESKMKWFDVPG NLIHAINAWDEDDAVVL+AP
Sbjct: 361 MIMEGRSPVGTDPSTVSRVGLIPRYADDESKMKWFDVPGFNLIHAINAWDEDDAVVLLAP 420

Query: 421 NILSVEHTLERTDLVHALMEKIRIDLKTGIVARRPLSTRNLDFGVLNPSYIGKKNRFVYA 480
           NILSVEHTLER DLVHAL+E++RIDLKTGIV RRPLSTRNLDFGV+NPSYIGKKNRFVYA
Sbjct: 421 NILSVEHTLERMDLVHALIEEVRIDLKTGIVTRRPLSTRNLDFGVINPSYIGKKNRFVYA 480

Query: 481 GVGDPMPKISGVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETAPEEDD 540
           GVGDPMPKISGVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETA EEDD
Sbjct: 481 GVGDPMPKISGVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETALEEDD 540

Query: 541 GYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 597
           GYVVSYVHDENSGES+FIVMDAKSP+LEIIA VKLPRRV YGFHGLFVKESDL KL
Sbjct: 541 GYVVSYVHDENSGESKFIVMDAKSPKLEIIAVVKLPRRVSYGFHGLFVKESDLRKL 586

BLAST of Tan0005556 vs. ExPASy TrEMBL
Match: A0A5A7TP93 (Putative carotenoid cleavage dioxygenase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold74G003220 PE=3 SV=1)

HSP 1 Score: 1055.8 bits (2729), Expect = 6.6e-305
Identity = 519/597 (86.93%), Postives = 553/597 (92.63%), Query Frame = 0

Query: 1   MGAISSPFFSGRNLLLSPPISISGPPVSTAISSVLTEENLKKTAPRPDVADSPSSSPSPS 60
           M +ISSPF S  NL+LSPPIS S PP+ST I SVLTE+  KK  P PD ADSPS S   S
Sbjct: 1   MDSISSPFLSRGNLILSPPISTSRPPISTPIYSVLTEQTAKKNTPPPD-ADSPSPS---S 60

Query: 61  LPRR-PPPPAIAKGPSTTRRLEPSLTARFFNAFDDLINNFINPPVTPSVDPRYVLANNFA 120
           LPRR PP PA+A+  S+TRR+EPSL AR FNAFDDLINNFINPP++PSVDPRY+LA+NFA
Sbjct: 61  LPRRSPPSPAMAR-VSSTRRVEPSLPARLFNAFDDLINNFINPPISPSVDPRYILADNFA 120

Query: 121 PVDELPPTDCEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLNSLRISNGRAV 180
           PVDELPPT+CEVI GSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGML+SLRISNGRAV
Sbjct: 121 PVDELPPTECEVIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAV 180

Query: 181 LCSRFVKTYKYTLERDAGYPLFPNVFSGFNGLTASAARGAVAAGRMLTGQYNPANGIGLA 240
           LCSR+VKTYKYTLERDAG+P+ PNVFSGFNGLTASAARGAV+ GR+LTGQYNPANGIGLA
Sbjct: 181 LCSRYVKTYKYTLERDAGHPVLPNVFSGFNGLTASAARGAVSFGRILTGQYNPANGIGLA 240

Query: 241 NTSLAFFGDRLYALGESDLPYLIRLTPNGDIETLGRHDFDGKLTMSMTAHPKLDSDTGEA 300
           NTSLAFFGDRLYALGESDLPYLIRL PNGDIETL RHDFDGKLT SMTAHPK+DSDTGEA
Sbjct: 241 NTSLAFFGDRLYALGESDLPYLIRLAPNGDIETLSRHDFDGKLTYSMTAHPKIDSDTGEA 300

Query: 301 FAFRYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRPTFLHDFAITKKYAVFTDIQIGFNPM 360
           FAFRYGPLPPFLT+FRFDKNGAKQSDVPI SMNRP+FLHDFAITKKYAVF DIQIG NP 
Sbjct: 301 FAFRYGPLPPFLTYFRFDKNGAKQSDVPILSMNRPSFLHDFAITKKYAVFADIQIGINPT 360

Query: 361 PMIIEGRSPVGSDPSKVSRIGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVLVA 420
            MIIEG SPVGSDPSK+SR+GLIPRYANDESKMKWFDVPGLNL+HAINAWDEDDAVV+VA
Sbjct: 361 QMIIEGGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLVHAINAWDEDDAVVIVA 420

Query: 421 PNILSVEHTLERTDLVHALMEKIRIDLKTGIVARRPLSTRNLDFGVLNPSYIGKKNRFVY 480
           PNILSVEH LER +LVH L+EK+RIDLKTGIV R PLSTRNLDFGV++PSY+GKKNRFVY
Sbjct: 421 PNILSVEHALERMNLVHGLVEKVRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKNRFVY 480

Query: 481 AGVGDPMPKISGVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETAPEED 540
           AG+GDPMPKISGV KLD+SQEERRDCIVA RIFGPGCYGGEPFFVPRERESSDETA EED
Sbjct: 481 AGIGDPMPKISGVAKLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDETAAEED 540

Query: 541 DGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 597
           DGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL
Sbjct: 541 DGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592

BLAST of Tan0005556 vs. ExPASy TrEMBL
Match: A0A5D3CY96 (Putative carotenoid cleavage dioxygenase 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21G002650 PE=3 SV=1)

HSP 1 Score: 1055.4 bits (2728), Expect = 8.6e-305
Identity = 518/597 (86.77%), Postives = 553/597 (92.63%), Query Frame = 0

Query: 1   MGAISSPFFSGRNLLLSPPISISGPPVSTAISSVLTEENLKKTAPRPDVADSPSSSPSPS 60
           M +ISSPF S  NL+LSPPIS S PP+ST I SVLTE+  KK  P PD ADSPS S   S
Sbjct: 1   MDSISSPFLSRGNLILSPPISTSRPPISTPIYSVLTEQTAKKNTPPPD-ADSPSPS---S 60

Query: 61  LPRR-PPPPAIAKGPSTTRRLEPSLTARFFNAFDDLINNFINPPVTPSVDPRYVLANNFA 120
           LPRR PP PA+A+  S+TRR+EPSL AR FNAFDDLINNFINPP++PSVDPRY+LA+NFA
Sbjct: 61  LPRRSPPSPAMAR-VSSTRRVEPSLPARLFNAFDDLINNFINPPISPSVDPRYILADNFA 120

Query: 121 PVDELPPTDCEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLNSLRISNGRAV 180
           PVDELPPT+CE+I GSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGML+SLRISNGRAV
Sbjct: 121 PVDELPPTECEIIYGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLHSLRISNGRAV 180

Query: 181 LCSRFVKTYKYTLERDAGYPLFPNVFSGFNGLTASAARGAVAAGRMLTGQYNPANGIGLA 240
           LCSR+VKTYKYTLERDAG+P+ PNVFSGFNGLTASAARGAV+ GR+LTGQYNPANGIGLA
Sbjct: 181 LCSRYVKTYKYTLERDAGHPVLPNVFSGFNGLTASAARGAVSFGRILTGQYNPANGIGLA 240

Query: 241 NTSLAFFGDRLYALGESDLPYLIRLTPNGDIETLGRHDFDGKLTMSMTAHPKLDSDTGEA 300
           NTSLAFFGDRLYALGESDLPYLIRL PNGDIETL RHDFDGKLT SMTAHPK+DSDTGEA
Sbjct: 241 NTSLAFFGDRLYALGESDLPYLIRLAPNGDIETLSRHDFDGKLTYSMTAHPKIDSDTGEA 300

Query: 301 FAFRYGPLPPFLTFFRFDKNGAKQSDVPIFSMNRPTFLHDFAITKKYAVFTDIQIGFNPM 360
           FAFRYGPLPPFLT+FRFDKNGAKQSDVPI SMNRP+FLHDFAITKKYAVF DIQIG NP 
Sbjct: 301 FAFRYGPLPPFLTYFRFDKNGAKQSDVPILSMNRPSFLHDFAITKKYAVFADIQIGINPT 360

Query: 361 PMIIEGRSPVGSDPSKVSRIGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVLVA 420
            MIIEG SPVGSDPSK+SR+GLIPRYANDESKMKWFDVPGLNL+HAINAWDEDDAVV+VA
Sbjct: 361 QMIIEGGSPVGSDPSKISRVGLIPRYANDESKMKWFDVPGLNLVHAINAWDEDDAVVIVA 420

Query: 421 PNILSVEHTLERTDLVHALMEKIRIDLKTGIVARRPLSTRNLDFGVLNPSYIGKKNRFVY 480
           PNILSVEH LER +LVH L+EK+RIDLKTGIV R PLSTRNLDFGV++PSY+GKKNRFVY
Sbjct: 421 PNILSVEHALERMNLVHGLVEKVRIDLKTGIVTRTPLSTRNLDFGVIHPSYVGKKNRFVY 480

Query: 481 AGVGDPMPKISGVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETAPEED 540
           AG+GDPMPKISGV KLD+SQEERRDCIVA RIFGPGCYGGEPFFVPRERESSDETA EED
Sbjct: 481 AGIGDPMPKISGVAKLDISQEERRDCIVACRIFGPGCYGGEPFFVPRERESSDETAAEED 540

Query: 541 DGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 597
           DGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL
Sbjct: 541 DGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 592

BLAST of Tan0005556 vs. TAIR 10
Match: AT4G19170.1 (nine-cis-epoxycarotenoid dioxygenase 4 )

HSP 1 Score: 789.6 bits (2038), Expect = 1.7e-228
Identity = 383/586 (65.36%), Postives = 467/586 (79.69%), Query Frame = 0

Query: 23  SGPPVSTAISSVLTEENLKKTAPRPDVADSPSSSPSPSLPRRPPPPAIAKGPSTT----- 82
           S  P    I+S + EE             SP ++PS +  RR  P  +    + T     
Sbjct: 25  SSSPTLLRINSAVVEER------------SPITNPSDNNDRRNKPKTLHNRTNHTLVSSP 84

Query: 83  --RRLEPSLTARFFNAFDDLINNFINPPVTPSVDPRYVLANNFAPV-DELPPTDCEVIQG 142
              R E +L    F   +D+IN FI+PP  PSVDP++VL++NFAPV DELPPTDCE+I G
Sbjct: 85  PKLRPEMTLATALFTTVEDVINTFIDPPSRPSVDPKHVLSDNFAPVLDELPPTDCEIIHG 144

Query: 143 SLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLNSLRISNGRAVLCSRFVKTYKYTLER 202
           +LP SLNGAYIRNGPNPQ+LPRGPYHLFDGDGML++++I NG+A LCSR+VKTYKY +E+
Sbjct: 145 TLPLSLNGAYIRNGPNPQFLPRGPYHLFDGDGMLHAIKIHNGKATLCSRYVKTYKYNVEK 204

Query: 203 DAGYPLFPNVFSGFNGLTASAARGAVAAGRMLTGQYNPANGIGLANTSLAFFGDRLYALG 262
             G P+ PNVFSGFNG+TAS ARGA+ A R+LTGQYNP NGIGLANTSLAFF +RL+ALG
Sbjct: 205 QTGAPVMPNVFSGFNGVTASVARGALTAARVLTGQYNPVNGIGLANTSLAFFSNRLFALG 264

Query: 263 ESDLPYLIRLTPNGDIETLGRHDFDGKLTMSMTAHPKLDSDTGEAFAFRYGPLPPFLTFF 322
           ESDLPY +RLT +GDIET+GR+DFDGKL MSMTAHPK D  TGE FAFRYGP+PPFLT+F
Sbjct: 265 ESDLPYAVRLTESGDIETIGRYDFDGKLAMSMTAHPKTDPITGETFAFRYGPVPPFLTYF 324

Query: 323 RFDKNGAKQSDVPIFSMNRPTFLHDFAITKKYAVFTDIQIG--FNPMPMIIEGRSPVGSD 382
           RFD  G KQ DVPIFSM  P+FLHDFAITK++A+F +IQ+G   N + +++EG SPVG+D
Sbjct: 325 RFDSAGKKQRDVPIFSMTSPSFLHDFAITKRHAIFAEIQLGMRMNMLDLVLEGGSPVGTD 384

Query: 383 PSKVSRIGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDD--AVVLVAPNILSVEHTLE 442
             K  R+G+IP+YA DES+MKWF+VPG N+IHAINAWDEDD  +VVL+APNI+S+EHTLE
Sbjct: 385 NGKTPRLGVIPKYAGDESEMKWFEVPGFNIIHAINAWDEDDGNSVVLIAPNIMSIEHTLE 444

Query: 443 RTDLVHALMEKIRIDLKTGIVARRPLSTRNLDFGVLNPSYIGKKNRFVYAGVGDPMPKIS 502
           R DLVHAL+EK++IDL TGIV R P+S RNLDF V+NP+++G+ +R+VYA +GDPMPKIS
Sbjct: 445 RMDLVHALVEKVKIDLVTGIVRRHPISARNLDFAVINPAFLGRCSRYVYAAIGDPMPKIS 504

Query: 503 GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETAPEEDDGYVVSYVHDE 562
           GVVKLDVS+ +R DC VA R++G GCYGGEPFFV R+  + +    EEDDGYVV+YVHDE
Sbjct: 505 GVVKLDVSKGDRDDCTVARRMYGSGCYGGEPFFVARDPGNPE---AEEDDGYVVTYVHDE 564

Query: 563 NSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNKL 597
            +GES+F+VMDAKSPELEI+AAV+LPRRVPYGFHGLFVKESDLNKL
Sbjct: 565 VTGESKFLVMDAKSPELEIVAAVRLPRRVPYGFHGLFVKESDLNKL 595

BLAST of Tan0005556 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 395.6 bits (1015), Expect = 7.1e-110
Identity = 226/583 (38.77%), Postives = 333/583 (57.12%), Query Frame = 0

Query: 19  PISISGPPVSTAISSVLTEENLKKTAPRPDVADSPSSSPSPSLPRRPPPPAIAKGPSTTR 78
           P  +  P  S+++S   T+   +K +    V+D+P+    P+ P  P P    K  S   
Sbjct: 20  PSDLDAPSPSSSVSFTNTKPRRRKLSAN-SVSDTPNLLNFPNYP-SPNPIIPEKDTSRWN 79

Query: 79  RLEPSLTARFFNAFDDLINNFINPPVTPSVDPRYVLANNFAPVDELPPTDCEVIQGSLPS 138
            L+ + +A    A   L+    + P+  +VDPR+ ++ N+APV E        + G +P 
Sbjct: 80  PLQRAASAALDFAETALLRRERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSVDGKIPD 139

Query: 139 SLNGAYIRNGPNPQYLPRGPYHLFDGDGMLNSLRISNGRAVLCSRFVKTYKYTLERDAGY 198
            ++G Y+RNG NP + P   +HLFDGDGM+++++I+NG A    RF +T +   E+  G 
Sbjct: 140 CIDGVYLRNGANPLFEPVSGHHLFDGDGMVHAVKITNGDASYSCRFTETERLVQEKQLGS 199

Query: 199 PLFPNVFSGFNGLTASAARGAVAAGRMLTGQYNPANGIGLANTSLAFFGDRLYALGESDL 258
           P+FP      +G  +  AR  +   R L G  N  NG G+AN  L +F DRL A+ E DL
Sbjct: 200 PIFPKAIGELHG-HSGIARLMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDL 259

Query: 259 PYLIRLTPNGDIETLGRHDFDGKLTMSMTAHPKLDSDTGEAFAFRYGPL-PPFLTFFRFD 318
           PY +R+T NGD+ET+GR DFDG+L+ +M AHPK+D  T E FA  Y  +  P+L +F+F 
Sbjct: 260 PYQVRVTDNGDLETIGRFDFDGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLKYFKFS 319

Query: 319 KNGAKQSDVPIFSMNRPTFLHDFAITKKYAVFTDIQIGFNPMPMIIEGRSPVGSDPSKVS 378
             G K  DV I  +  PT +HDFAIT+ + V  D Q+ F    M + G+SPV  D  K+S
Sbjct: 320 PEGEKSPDVEI-PLASPTMMHDFAITENFVVIPDQQVVFKLSDMFL-GKSPVKYDGEKIS 379

Query: 379 RIGLIPRYANDESKMKWFDVPGLNLIHAINAWD--EDDAVVLVAPNILSVEHTLERTD-L 438
           R G++PR A D S+M W + P     H  NAW+  E D VV++   +   +      D  
Sbjct: 380 RFGILPRNAKDASEMVWVESPETFCFHLWNAWESPETDEVVVIGSCMTPADSIFNECDEQ 439

Query: 439 VHALMEKIRIDLKTGIVARRPL----STRNLDFGVLNPSYIGKKNRFVYAGVGDPMPKIS 498
           +++++ +IR++LKTG   RR +       NL+ G++N + +G+K R+ Y  + +P PK+S
Sbjct: 440 LNSVLSEIRLNLKTGKSTRRTIIPGSVQMNLEAGMVNRNLLGRKTRYAYLAIAEPWPKVS 499

Query: 499 GVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETAPEEDDGYVVSYVHDE 558
           G  K+D+S  E     V +  +G   YGGEPFF+PR  ES       EDDGY++S+VHDE
Sbjct: 500 GFAKVDLSTGE-----VKNHFYGGKKYGGEPFFLPRGLESDG-----EDDGYIMSFVHDE 559

Query: 559 NSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDL 594
            S ES   +++A + ELE  A VKLP RVPYGFHG FV  +D+
Sbjct: 560 ESWESELHIVNAVTLELE--ATVKLPSRVPYGFHGTFVNSADM 585

BLAST of Tan0005556 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 386.7 bits (992), Expect = 3.3e-107
Identity = 223/592 (37.67%), Postives = 341/592 (57.60%), Query Frame = 0

Query: 25  PPVSTAISSVLTEENLKKTAPRP----DVADSPSSSPSPSLPRRPP-PPAIAKGP----S 84
           PP+S++ SS L+  +    A R     +V+ +  + P+   P++    PAI   P    S
Sbjct: 22  PPLSSSQSSDLSYCSSLPMASRVTRKLNVSSALHTPPALHFPKQSSNSPAIVVKPKAKES 81

Query: 85  TTRRL---EPSLTARFFNAFDDLINNFINPPVTPSVDPRYVLANNFAPVDELPPTDCEVI 144
            T+++   + +  A    A   L+++    P+  + DP   +A NFAPV+E P      +
Sbjct: 82  NTKQMNLFQRAAAAALDAAEGFLVSHEKLHPLPKTADPSVQIAGNFAPVNEQPVRRNLPV 141

Query: 145 QGSLPSSLNGAYIRNGPNPQYLPRGPYHLFDGDGMLNSLRISNGRAVLCSRFVKTYKYTL 204
            G LP S+ G Y+RNG NP + P   +H FDGDGM+++++  +G A    RF +T ++  
Sbjct: 142 VGKLPDSIKGVYVRNGANPLHEPVTGHHFFDGDGMVHAVKFEHGSASYACRFTQTNRFVQ 201

Query: 205 ERDAGYPLFPNVFSGFNGLTASAARGAVAAGRMLTGQYNPANGIGLANTSLAFFGDRLYA 264
           ER  G P+FP      +G T   AR  +   R   G  +PA+G G+AN  L +F  RL A
Sbjct: 202 ERQLGRPVFPKAIGELHGHT-GIARLMLFYARAAAGIVDPAHGTGVANAGLVYFNGRLLA 261

Query: 265 LGESDLPYLIRLTPNGDIETLGRHDFDGKLTMSMTAHPKLDSDTGEAFAFRYGPL-PPFL 324
           + E DLPY +++TPNGD++T+GR DFDG+L  +M AHPK+D ++GE FA  Y  +  P+L
Sbjct: 262 MSEDDLPYQVQITPNGDLKTVGRFDFDGQLESTMIAHPKVDPESGELFALSYDVVSKPYL 321

Query: 325 TFFRFDKNGAKQSDVPIFSMNRPTFLHDFAITKKYAVFTDIQIGFNPMPMIIEGRSPVGS 384
            +FRF  +G K  DV I  +++PT +HDFAIT+ + V  D Q+ F  +P +I G SPV  
Sbjct: 322 KYFRFSPDGTKSPDVEI-QLDQPTMMHDFAITENFVVVPDQQVVFK-LPEMIRGGSPVVY 381

Query: 385 DPSKVSRIGLIPRYANDESKMKWFDVPGLNLIHAINAWD--EDDAVVLVAPNILSVEHTL 444
           D +KV+R G++ +YA D S +KW D P     H  NAW+  E D VV++   +   +   
Sbjct: 382 DKNKVARFGILDKYAEDSSNIKWIDAPDCFCFHLWNAWEEPETDEVVVIGSCMTPPDSIF 441

Query: 445 ERTD-LVHALMEKIRIDLKTGIVARRPLSTR-----NLDFGVLNPSYIGKKNRFVYAGVG 504
             +D  + +++ +IR++LKTG   RRP+ +      NL+ G++N + +G+K +F Y  + 
Sbjct: 442 NESDENLKSVLSEIRLNLKTGESTRRPIISNEDQQVNLEAGMVNRNMLGRKTKFAYLALA 501

Query: 505 DPMPKISGVVKLDVSQEERRDCIVASRIFGPGCYGGEPFFVPRERESSDETAPEEDDGYV 564
           +P PK+SG  K+D++  E     V   ++G   YGGEP F+P E         EED+GY+
Sbjct: 502 EPWPKVSGFAKVDLTTGE-----VKKHLYGDNRYGGEPLFLPGE-------GGEEDEGYI 561

Query: 565 VSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNK 596
           + +VHDE + +S   +++A S  LE+ A VKLP RVPYGFHG F+   DL K
Sbjct: 562 LCFVHDEKTWKSELQIVNAVS--LEVEATVKLPSRVPYGFHGTFIGADDLAK 596

BLAST of Tan0005556 vs. TAIR 10
Match: AT3G63520.1 (carotenoid cleavage dioxygenase 1 )

HSP 1 Score: 375.2 bits (962), Expect = 1.0e-103
Identity = 205/533 (38.46%), Postives = 304/533 (57.04%), Query Frame = 0

Query: 80  LEPSLTARFFNAFDDLINNFINPPVTPSVDPRYVLANNFAPV-DELPPTDCEVIQGSLPS 139
           + P  +  F +   DL+   +   +  +  P + L+ NFAP+ DE PP     + G LP 
Sbjct: 14  VHPRPSKGFSSKLLDLLERLVVKLMHDASLPLHYLSGNFAPIRDETPPVKDLPVHGFLPE 73

Query: 140 SLNGAYIRNGPNPQYLPRGPYHLFDGDGMLNSLRISNGRAVLCSRFVKTYKYTLERDAGY 199
            LNG ++R GPNP++     YH FDGDGM++ +RI +G+A   SR+VKT +   E   G 
Sbjct: 74  CLNGEFVRVGPNPKFDAVAGYHWFDGDGMIHGVRIKDGKATYVSRYVKTSRLKQEEFFGA 133

Query: 200 PLFPNV--FSGFNGLTASAARGAVAAGRMLTGQYNPANGIGLANTSLAFFGDRLYALGES 259
             F  +    GF GL     +      ++L   Y    G G ANT+L +   +L AL E+
Sbjct: 134 AKFMKIGDLKGFFGLLMVNVQQLRTKLKILDNTY----GNGTANTALVYHHGKLLALQEA 193

Query: 260 DLPYLIRLTPNGDIETLGRHDFDGKLTMSMTAHPKLDSDTGEAFAFRYGPLPPFLTFFRF 319
           D PY+I++  +GD++TLG  D+D +LT S TAHPK+D  TGE F F Y   PP+LT+   
Sbjct: 194 DKPYVIKVLEDGDLQTLGIIDYDKRLTHSFTAHPKVDPVTGEMFTFGYSHTPPYLTYRVI 253

Query: 320 DKNGAKQSDVPIFSMNRPTFLHDFAITKKYAVFTDIQIGFNPMPMIIEGRSPVGSDPSKV 379
            K+G     VPI +++ P  +HDFAIT+ YA+F D+ + F P  M+ E +     DP+K 
Sbjct: 254 SKDGIMHDPVPI-TISEPIMMHDFAITETYAIFMDLPMHFRPKEMVKEKKMIYSFDPTKK 313

Query: 380 SRIGLIPRYANDESKMKWFDVPGLNLIHAINAWDEDDAVVLVA-----PNILSVEHTL-E 439
           +R G++PRYA DE  ++WF++P   + H  NAW+E+D VVL+      P++  V   + E
Sbjct: 314 ARFGVLPRYAKDELMIRWFELPNCFIFHNANAWEEEDEVVLITCRLENPDLDMVSGKVKE 373

Query: 440 RTDLVHALMEKIRIDLKTGIVARRPLSTRNLDFGVLNPSYIGKKNRFVYAGVGDPMPKIS 499
           + +     + ++R ++KTG  +++ LS   +DF  +N  Y GKK R+VY  + D + K++
Sbjct: 374 KLENFGNELYEMRFNMKTGSASQKKLSASAVDFPRINECYTGKKQRYVYGTILDSIAKVT 433

Query: 500 GVVKLDVSQEE---RRDCIVASRI-----FGPGCYGGEPFFVPRERESSDETAPEEDDGY 559
           G++K D+  E    +R   V   I      G G YG E  +VPRE         EEDDGY
Sbjct: 434 GIIKFDLHAEAETGKRMLEVGGNIKGIYDLGEGRYGSEAIYVPRE-------TAEEDDGY 493

Query: 560 VVSYVHDENSGESRFIVMDAKSPELEIIAAVKLPRRVPYGFHGLFVKESDLNK 596
           ++ +VHDEN+G+S   V+DAK+   E +A V+LP RVPYGFH LFV E  L +
Sbjct: 494 LIFFVHDENTGKSCVTVIDAKTMSAEPVAVVELPHRVPYGFHALFVTEEQLQE 534

BLAST of Tan0005556 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 369.8 bits (948), Expect = 4.2e-102
Identity = 196/499 (39.28%), Postives = 292/499 (58.52%), Query Frame = 0

Query: 103 PVTPSVDPRYVLANNFAPVDELPPTDCEVIQGSLPSSLNGAYIRNGPNPQYLPRGPYHLF 162
           P   + DP   +A NF PV E P      + G++P  + G Y+RNG NP + P   +HLF
Sbjct: 172 PHPKTADPAVQIAGNFFPVPEKPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLF 231

Query: 163 DGDGMLNSLRISNGRAVLCSRFVKTYKYTLERDAGYPLFPNVFSGFNGLTASAARGAVAA 222
           DGDGM++++R  NG      RF +T +   ER+ G P+FP      +G     A+  +  
Sbjct: 232 DGDGMVHAVRFDNGSVSYACRFTETNRLVQERECGRPVFPKAIGELHG-HLGIAKLMLFN 291

Query: 223 GRMLTGQYNPANGIGLANTSLAFFGDRLYALGESDLPYLIRLTPNGDIETLGRHDFDGKL 282
            R L G  +P  G+G+AN  L +F   L A+ E DLPY +++T  GD+ET GR+DFDG+L
Sbjct: 292 TRGLFGLVDPTGGLGVANAGLVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQL 351

Query: 283 TMSMTAHPKLDSDTGEAFAFRYGPL-PPFLTFFRFDKNGAKQSDVPIFSMNRPTFLHDFA 342
             +M AHPK+D +T E FA  Y  +  P+L +FRF  +G K  DV I  +++PT +HDFA
Sbjct: 352 KSTMIAHPKIDPETRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEI-PLDQPTMIHDFA 411

Query: 343 ITKKYAVFTDIQIGFNPMPMIIEGRSPVGSDPSKVSRIGLIPRYANDESKMKWFDVPGLN 402
           IT+ + V  D Q+ F  +P +I G SPV  D  K SR G++ + A D S ++W +VP   
Sbjct: 412 ITENFVVIPDQQVVFR-LPEMIRGGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVPDCF 471

Query: 403 LIHAINAWD--EDDAVVLVAPNILSVEHTL-ERTDLVHALMEKIRIDLKTGIVARRPLST 462
             H  N+W+  E D VV++   +   +    E  + + +++ +IR++LKTG   RRP+ +
Sbjct: 472 CFHLWNSWEEPETDEVVVIGSCMTPPDSIFNEHDETLQSVLSEIRLNLKTGESTRRPVIS 531

Query: 463 R--NLDFGVLNPSYIGKKNRFVYAGVGDPMPKISGVVKLDVSQEERRDCIVASRIFGPGC 522
              NL+ G++N + +G+K R+ Y  + +P PK+SG  K+D+S  E     +   I+G G 
Sbjct: 532 EQVNLEAGMVNRNLLGRKTRYAYLALTEPWPKVSGFAKVDLSTGE-----IRKYIYGEGK 591

Query: 523 YGGEPFFVPRERESSDETAPEEDDGYVVSYVHDENSGESRFIVMDAKSPELEIIAAVKLP 582
           YGGEP F+P    S D    EED GY++ +VHDE   +S   +++A + +LE  A V LP
Sbjct: 592 YGGEPLFLP----SGD---GEEDGGYIMVFVHDEEKVKSELQLINAVNMKLE--ATVTLP 651

Query: 583 RRVPYGFHGLFVKESDLNK 596
            RVPYGFHG F+ + DL+K
Sbjct: 652 SRVPYGFHGTFISKEDLSK 653