Tan0005553 (gene) Snake gourd v1

Overview
NameTan0005553
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionPectinesterase
LocationLG07: 4599782 .. 4603275 (-)
RNA-Seq ExpressionTan0005553
SyntenyTan0005553
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTGAAAAGCTCCATTTTTGGATCCTTGTGATTTATTCATTGTCCATTTTATTATCAATGTCTCCATGTTTCTCAAGAAGTCCAAACGCCATAGATTTATGGTGCAGCCGAACCCCATATCCGGACGTGTGCAAACACTTGTTTAAAGACGCGAAATTGGACCCAACAAAGCCCTCCGAGTTAAAAAAAACAGCTCTCAAAATCGCCATGGAAAGAGCAGTGACAGCCGAAACACTCACGAAGCAGCTCGGCGAAAAATGCCGAAGCCAAAAAGAGAGAGCCGCATGGGCCGACTGCTTGGAGCTCTACCAAAACACAATCCTCCAACTCAACAGAACATTCTCCGACAAATACTGCTCCGATTTCGACATCCAAACGTGGCTCAGCTCCGCGTTGACCAATCTCGAGACATGTCGTGCCGGGTTCGTTGACATGAATGTCAAGGACTACGACGTCGTTTTCCCCTTCCTGGCGAGGAATAATAACGTGACGCAGTTGATTAGCAATAGTTTAGCGATGAATAACTGCTCGGAGACGGAGATGGGTGGTTATGATCATGGGGGGTTTCCGACGTGGGTTCGGGGTGGAGACCGGCGGCTGCTGCAGGCGGAGGGGGTGCCTAAGGCAGACTTGGTGGTGGCGCAGGATGGGTCGGGGAATTATAGGACGGTGCAGGCGGCGGTGGATGGGATTAAATTAGGAAGTGGGAGATTTGTAATACATGTGAAAAAAGGGGTTTATAAAGAGAATGTTGTGATTGGGAATAAGATGAAGAATTTGATGCTTGTTGGAGATGGATTGAAGTATACTATTATTACTGGGAGTAGAAGTGTTGGTGGTGGTTCCACTACTTTCAACTCTGCTACTGTTGGTCAGTTTCTTCTCTCTCTCTCTATATCTTTTTTTCTCTAACTACATTATTTATGAAGTTTAATTTTAATATTTTTTTTTTTCTATTTATATCTACAACTTTTCAAAACTATCTATTTTCATTATTTGTCACCTTAATATATCTAATATCTATCTACCACCCAATAAACAACTTATTTGATTAGCGTATCACTTCTATGTATGCCATTGTCATACATGTTCTATCTTATCTATTTAGATTCATACACTTTGATATTATATTATAAAAATCTAAAATTTCATCTTAAAATCGATGGATAAGAAAAAGAATCATTCATGTATCTTTATAAAAATTGTAACGTTCTTTTTATCTTTTCGAGGGTGGAATCAAGTGAAAGTGCAAGTACAGCCTGAGTTGCACTTATCTATGGTGCAACCCATTTTCAAACCACAAAAGTTTCTTTTATAAAAAATTAAAATAATTTTTTTTTTTTTTGTGGTTCTACGTTGGGTTGCATATATATTAGAGCGGCACCCAAGTATTTTTTTACAATAAATTAATATTGATGTTTAGTTTTGAAAGTTCTGCATGGACAATGATGAGATAAATTTGAAAGCTTAAGAAATAAAATGAAATTTAGACGGGTAGTTTCTAATTGGATTAACTCCCATAAGAAATTATATTTTCGAGTGTTATATGGAAGAGGGCTTATTTGATAGGCAATCTAAAAACAGAATTTTGAAAACAATAAAAAACATAAAGTTATATTTCATATTTTCATATATGTGTTTGATAGTAGATTTAAAAATTGGATTCTAATTTAAGCAGTGGTTGAAAATGTGTTCTATAAATACATTACCATGAAATTAATTGTACTGGTAAAATGAAATATTAGATTGAATATAAATCATTATTAATTAATTTATGAATGTGTGTTTTTTTAATTAAAACATAAAAATGTTGCTTTTCAACACTTTCACTATTTATATCACAAAATTCGAAAATAGTTTTTCAAAACACACCTGCCATATATATATATATTCACTGATCTCATATGCCAAGTACTCTTTTTCTTCTTTATGGTAATACGAAAATTAGTTTTGCTAACAAAATAAAAGAAAGTCTTGTTTGTTCTTTTAATTTTTTATTTTTGAATTTAACGAGGAGAAGATGGAAAGATTTGAACTTCCAACTTCTTACCAAAAGGTATATATATTTTTTAGTTATATTTAGGTTGACGAAACTAATTTTATAGTTAAATTAAGGGTGGATTTTGAAAAAAGAAAATTTTATGTGGAATTCGGTAGGTATAATAAGGGGTCGTTTGGATTGAGGAGTTGTGAGGAGTTAATAACTCCCCAAATTCTTTGTTTGGCCCAATGAGTTATTAACTACTAGGTCTTACAGATTTATTAACTTCTTCTTGTAGGTCCTACATATTTATTAACTTATTTCCCAACTCTTTCTTATGAGCCCCACAGATTTATTAACTATTTTTCTAACTTTTCATAACTCTTTTTTAAACTGTTACTTCCTGCAACTCTTTGCCCCAAACACAGTTTCTCATAACTCCAAAGTAAACAAATTTGCACCCAAAACACAGACTTCAAAACTCCTCAGACTTTTTTACTCCCCCAGACTTATTTACTCCTACTACCTACAACTTTTGCTCCCCAACTCCTAAATCCAAACGGCTCCTAAGTATCTTTATAATTATATATATAAGTATCGAAAATTACTTAATATTTATTGAATTCTAACCCTGGACTTATCACATAAACTTGAGGATCATGTATCTTTATTGAGTAAGCTTGAAAACAAAAGTCTATATTAAAAAATCTTGAACGCTATCCATGGATATAAGACATTCCCATATCCCAATATCATTCTCCTCAATATAGCAACCCTTTGTAAATGCCTTACTGAGAGTTTGCGACCTTCTTGATCAGCTGTGACTGGGGAAGGTTTCATCGCCCGTGGCATCACATTTCGCAACACGGCCGGACCCGAAAACCACCAGGCAGTAGCCCTGCGGTCGGGCTCCGATCTCTCCGTCTTCTACCGCTGTGGCTTCGAGGGTTATCAAGACACTCTCTACGTCCACTCGCAAAGACAATTCTACAGAGAATGCTACATCTACGGCACGGTCGATTTCATCTTCGGCAATGCAGCCGTGGTTCTCCAAAACTGCATGATCTACGCAAGAAAACCTATCCCAGGCCAGCAATGCGTGGTGACAGCCCAAGGCCGGACCGACCCGAACCAGAACACGGGGATCTCGATCCACAACTCGAGAGTGATGGCCTCGGACGACCTCAAACCGGCGCTAAGCTCAGTGAGGACGTATTTGGGGAGACCATGGAAGGAGTACTCTCGCACAGTTTACTTGCAAAGCTTCATGGACAGCTTGGTGAACCCTGCAGGGTGGCTTGAATGGACTGGAAATTTTGCTCTCAACACATTGTATTATGGAGAGTACAAGAATTTTGGACCTGCTTCTTCTACTGCCAATAGAGTGAAATGGAAGGGTTATAGAGTGATCACAAGTGTCACAGAAGCTTCAAAGTTTACTGTTCAAAATTTTATTGCTGGCAAATCATGGCTGCCTCCAACTGGAGTTCCATTCACTGCTTCCCTTTGA

mRNA sequence

ATGGCTGAAAAGCTCCATTTTTGGATCCTTGTGATTTATTCATTGTCCATTTTATTATCAATGTCTCCATGTTTCTCAAGAAGTCCAAACGCCATAGATTTATGGTGCAGCCGAACCCCATATCCGGACGTGTGCAAACACTTGTTTAAAGACGCGAAATTGGACCCAACAAAGCCCTCCGAGTTAAAAAAAACAGCTCTCAAAATCGCCATGGAAAGAGCAGTGACAGCCGAAACACTCACGAAGCAGCTCGGCGAAAAATGCCGAAGCCAAAAAGAGAGAGCCGCATGGGCCGACTGCTTGGAGCTCTACCAAAACACAATCCTCCAACTCAACAGAACATTCTCCGACAAATACTGCTCCGATTTCGACATCCAAACGTGGCTCAGCTCCGCGTTGACCAATCTCGAGACATGTCGTGCCGGGTTCGTTGACATGAATGTCAAGGACTACGACGTCGTTTTCCCCTTCCTGGCGAGGAATAATAACGTGACGCAGTTGATTAGCAATAGTTTAGCGATGAATAACTGCTCGGAGACGGAGATGGGTGGTTATGATCATGGGGGGTTTCCGACGTGGGTTCGGGGTGGAGACCGGCGGCTGCTGCAGGCGGAGGGGGTGCCTAAGGCAGACTTGGTGGTGGCGCAGGATGGGTCGGGGAATTATAGGACGGTGCAGGCGGCGGTGGATGGGATTAAATTAGGAAGTGGGAGATTTGTAATACATGTGAAAAAAGGGGTTTATAAAGAGAATGTTGTGATTGGGAATAAGATGAAGAATTTGATGCTTGTTGGAGATGGATTGAAGTATACTATTATTACTGGGAGTAGAAGTGTTGGTGGTGGTTCCACTACTTTCAACTCTGCTACTGTTGCTGTGACTGGGGAAGGTTTCATCGCCCGTGGCATCACATTTCGCAACACGGCCGGACCCGAAAACCACCAGGCAGTAGCCCTGCGGTCGGGCTCCGATCTCTCCGTCTTCTACCGCTGTGGCTTCGAGGGTTATCAAGACACTCTCTACGTCCACTCGCAAAGACAATTCTACAGAGAATGCTACATCTACGGCACGGTCGATTTCATCTTCGGCAATGCAGCCGTGGTTCTCCAAAACTGCATGATCTACGCAAGAAAACCTATCCCAGGCCAGCAATGCGTGGTGACAGCCCAAGGCCGGACCGACCCGAACCAGAACACGGGGATCTCGATCCACAACTCGAGAGTGATGGCCTCGGACGACCTCAAACCGGCGCTAAGCTCAGTGAGGACGTATTTGGGGAGACCATGGAAGGAGTACTCTCGCACAGTTTACTTGCAAAGCTTCATGGACAGCTTGGTGAACCCTGCAGGGTGGCTTGAATGGACTGGAAATTTTGCTCTCAACACATTGTATTATGGAGAGTACAAGAATTTTGGACCTGCTTCTTCTACTGCCAATAGAGTGAAATGGAAGGGTTATAGAGTGATCACAAGTGTCACAGAAGCTTCAAAGTTTACTGTTCAAAATTTTATTGCTGGCAAATCATGGCTGCCTCCAACTGGAGTTCCATTCACTGCTTCCCTTTGA

Coding sequence (CDS)

ATGGCTGAAAAGCTCCATTTTTGGATCCTTGTGATTTATTCATTGTCCATTTTATTATCAATGTCTCCATGTTTCTCAAGAAGTCCAAACGCCATAGATTTATGGTGCAGCCGAACCCCATATCCGGACGTGTGCAAACACTTGTTTAAAGACGCGAAATTGGACCCAACAAAGCCCTCCGAGTTAAAAAAAACAGCTCTCAAAATCGCCATGGAAAGAGCAGTGACAGCCGAAACACTCACGAAGCAGCTCGGCGAAAAATGCCGAAGCCAAAAAGAGAGAGCCGCATGGGCCGACTGCTTGGAGCTCTACCAAAACACAATCCTCCAACTCAACAGAACATTCTCCGACAAATACTGCTCCGATTTCGACATCCAAACGTGGCTCAGCTCCGCGTTGACCAATCTCGAGACATGTCGTGCCGGGTTCGTTGACATGAATGTCAAGGACTACGACGTCGTTTTCCCCTTCCTGGCGAGGAATAATAACGTGACGCAGTTGATTAGCAATAGTTTAGCGATGAATAACTGCTCGGAGACGGAGATGGGTGGTTATGATCATGGGGGGTTTCCGACGTGGGTTCGGGGTGGAGACCGGCGGCTGCTGCAGGCGGAGGGGGTGCCTAAGGCAGACTTGGTGGTGGCGCAGGATGGGTCGGGGAATTATAGGACGGTGCAGGCGGCGGTGGATGGGATTAAATTAGGAAGTGGGAGATTTGTAATACATGTGAAAAAAGGGGTTTATAAAGAGAATGTTGTGATTGGGAATAAGATGAAGAATTTGATGCTTGTTGGAGATGGATTGAAGTATACTATTATTACTGGGAGTAGAAGTGTTGGTGGTGGTTCCACTACTTTCAACTCTGCTACTGTTGCTGTGACTGGGGAAGGTTTCATCGCCCGTGGCATCACATTTCGCAACACGGCCGGACCCGAAAACCACCAGGCAGTAGCCCTGCGGTCGGGCTCCGATCTCTCCGTCTTCTACCGCTGTGGCTTCGAGGGTTATCAAGACACTCTCTACGTCCACTCGCAAAGACAATTCTACAGAGAATGCTACATCTACGGCACGGTCGATTTCATCTTCGGCAATGCAGCCGTGGTTCTCCAAAACTGCATGATCTACGCAAGAAAACCTATCCCAGGCCAGCAATGCGTGGTGACAGCCCAAGGCCGGACCGACCCGAACCAGAACACGGGGATCTCGATCCACAACTCGAGAGTGATGGCCTCGGACGACCTCAAACCGGCGCTAAGCTCAGTGAGGACGTATTTGGGGAGACCATGGAAGGAGTACTCTCGCACAGTTTACTTGCAAAGCTTCATGGACAGCTTGGTGAACCCTGCAGGGTGGCTTGAATGGACTGGAAATTTTGCTCTCAACACATTGTATTATGGAGAGTACAAGAATTTTGGACCTGCTTCTTCTACTGCCAATAGAGTGAAATGGAAGGGTTATAGAGTGATCACAAGTGTCACAGAAGCTTCAAAGTTTACTGTTCAAAATTTTATTGCTGGCAAATCATGGCTGCCTCCAACTGGAGTTCCATTCACTGCTTCCCTTTGA

Protein sequence

MAEKLHFWILVIYSLSILLSMSPCFSRSPNAIDLWCSRTPYPDVCKHLFKDAKLDPTKPSELKKTALKIAMERAVTAETLTKQLGEKCRSQKERAAWADCLELYQNTILQLNRTFSDKYCSDFDIQTWLSSALTNLETCRAGFVDMNVKDYDVVFPFLARNNNVTQLISNSLAMNNCSETEMGGYDHGGFPTWVRGGDRRLLQAEGVPKADLVVAQDGSGNYRTVQAAVDGIKLGSGRFVIHVKKGVYKENVVIGNKMKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFRNTAGPENHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPALSSVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWTGNFALNTLYYGEYKNFGPASSTANRVKWKGYRVITSVTEASKFTVQNFIAGKSWLPPTGVPFTASL
Homology
BLAST of Tan0005553 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 9.8e-177
Identity = 319/518 (61.58%), Postives = 392/518 (75.68%), Query Frame = 0

Query: 9   ILVIYSLSILLSMSPCFSRSPNAIDLWCSRTPYPDVCKHLFKDAKLDPT---KPSELKKT 68
           IL+  SL +       F  SP  +  WC +TP P  C++ F   K D T   + ++  K 
Sbjct: 5   ILITVSLVLFSLSHTSFGYSPEEVKSWCGKTPNPQPCEY-FLTQKTDVTSIKQDTDFYKI 64

Query: 69  ALKIAMERAVTAETLTKQLGEKCRSQKERAAWADCLELYQNTILQLNRTF-SDKYCSDFD 128
           +L++A+ERA TA++ T  LG KCR+++E+AAW DC ELY+ T+L+LN+T  S   C+  D
Sbjct: 65  SLQLALERATTAQSRTYTLGSKCRNEREKAAWEDCRELYELTVLKLNQTSNSSPGCTKVD 124

Query: 129 IQTWLSSALTNLETCRAGFVDMNVKDYDVVFPFLARNNNVTQLISNSLAMNNCSETEMGG 188
            QTWLS+ALTNLETCRA   D+ V +Y  V P L  +NNVT+LISN+L++N     E   
Sbjct: 125 KQTWLSTALTNLETCRASLEDLGVPEY--VLPLL--SNNVTKLISNTLSLNKVPYNEPSY 184

Query: 189 YDHGGFPTWVRGGDRRLLQAEGVPKADLVVAQDGSGNYRTVQAAVDGI-KLGSGRFVIHV 248
            D  GFPTWV+ GDR+LLQ    P+A++VVAQDGSGN +T+Q AV    + G  R+VI++
Sbjct: 185 KD--GFPTWVKPGDRKLLQT--TPRANIVVAQDGSGNVKTIQEAVAAASRAGGSRYVIYI 244

Query: 249 KKGVYKENVVIGNKMKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGI 308
           K G Y EN+ +  K+KN+M VGDG+  TIITGS+SVGGG+TTF SATVAV G+ FIAR I
Sbjct: 245 KAGTYNENIEV--KLKNIMFVGDGIGKTIITGSKSVGGGATTFKSATVAVVGDNFIARDI 304

Query: 309 TFRNTAGPENHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFG 368
           T RNTAGP NHQAVALRSGSDLSVFYRC FEGYQDTLYVHSQRQFYREC IYGTVDFIFG
Sbjct: 305 TIRNTAGPNNHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECDIYGTVDFIFG 364

Query: 369 NAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPALSSVRT 428
           NAAVVLQNC I+ARKP P +   +TAQGRTDPNQ+TGI IHN RV A+ DLKP  SSV+T
Sbjct: 365 NAAVVLQNCNIFARKP-PNRTNTLTAQGRTDPNQSTGIIIHNCRVTAASDLKPVQSSVKT 424

Query: 429 YLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWTGNFALNTLYYGEYKNFGPASSTANRVKW 488
           +LGRPWK+YSRTVY+++F+DSL+NPAGW+EW+G+FALNTLYY EY N GP SSTANRVKW
Sbjct: 425 FLGRPWKQYSRTVYIKTFLDSLINPAGWMEWSGDFALNTLYYAEYMNTGPGSSTANRVKW 484

Query: 489 KGYRVITSVTEASKFTVQNFIAGKSWLPPTGVPFTASL 522
           +GY V+TS ++ S+FTV NFIAG SWLP T VPFT+ L
Sbjct: 485 RGYHVLTSPSQVSQFTVGNFIAGNSWLPATNVPFTSGL 510

BLAST of Tan0005553 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 565.5 bits (1456), Expect = 6.4e-160
Identity = 288/515 (55.92%), Postives = 380/515 (73.79%), Query Frame = 0

Query: 10  LVIYSLSILLSMSPCFSRSPNAIDLWCSRTPYPDVCKH-LFKDAKLDPTK-PSELKKTAL 69
           ++ + +  +L  S     +   +  WCS+TP P  C++ L  ++  +P K  SE  K ++
Sbjct: 8   IINFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISM 67

Query: 70  KIAMERAVTAETLTKQLGEKCRSQKERAAWADCLELYQNTILQLNRTFSDKY-CSDFDIQ 129
           K+ ++RA+ A+T    LG KCR  +E+AAW DC++LY  T+ ++N T      CS  D Q
Sbjct: 68  KLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQ 127

Query: 130 TWLSSALTNLETCRAGFVDMNVKDYDVVFPFLARNNNVTQLISNSLAMNNCSETEMGGYD 189
           TWLS+ALTNL+TCRAGF+++ V   D+V P +  +NNV+ L+ N+LA+N          +
Sbjct: 128 TWLSTALTNLDTCRAGFLELGVT--DIVLPLM--SNNVSNLLCNTLAINKV-PFNYTPPE 187

Query: 190 HGGFPTWVRGGDRRLLQAEGVPKADLVVAQDGSGNYRTVQAAVDGIKLGSGRFVIHVKKG 249
             GFP+WV+ GDR+LLQ+   PK + VVA+DGSGN++T++ A+D    GSGRFVI+VK+G
Sbjct: 188 KDGFPSWVKPGDRKLLQS-STPKDNAVVAKDGSGNFKTIKEAIDAAS-GSGRFVIYVKQG 247

Query: 250 VYKENVVIGNKMKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFR 309
           VY EN+ I  + KN+ML GDG+  TIITGS+SVGGG+TTFNSATVA  G+GFIARGITFR
Sbjct: 248 VYSENLEI--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFR 307

Query: 310 NTAGPENHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAA 369
           NTAG  N QAVALRSGSDLSVFY+C FE YQDTLYVHS RQFYR+C +YGTVDFIFGNAA
Sbjct: 308 NTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAA 367

Query: 370 VVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPALSSVRTYLG 429
            VLQNC I+AR+P   +   +TAQGR+DPNQNTGI IHNSRV A+ DL+P L S +TYLG
Sbjct: 368 AVLQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLG 427

Query: 430 RPWKEYSRTVYLQSFMDSLVNPAGWLEWTGNFALNTLYYGEYKNFGPASSTANRVKWKGY 489
           RPW++YSRTV++++ +DSL++P GWLEW GNFAL TL+Y E++N GP +ST+ RV W G+
Sbjct: 428 RPWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGF 487

Query: 490 RVITSVTEASKFTVQNFIAGKSWLPPTGVPFTASL 522
           RV+ S +EASKFTV  F+AG SW+ P+ VPFT+ L
Sbjct: 488 RVLGSASEASKFTVGTFLAGGSWI-PSSVPFTSGL 511

BLAST of Tan0005553 vs. ExPASy Swiss-Prot
Match: Q9FHN5 (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 545.4 bits (1404), Expect = 6.9e-154
Identity = 287/540 (53.15%), Postives = 374/540 (69.26%), Query Frame = 0

Query: 1   MAEKLHFWILVIYSLSILLSMSPCFSRSPNAIDLWCSRTPYPDVCKHLFKDAK--LDPTK 60
           M +KL    L +  L +LL + P  + +      WC +TPYPD CK  FK+      PT+
Sbjct: 4   MMQKLSILFLHLI-LLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQQPTQ 63

Query: 61  PSELKKTALKIAMERAVTAETLTKQLGEKCRSQKERAAWADCLELYQNTILQLNRTF--- 120
            SE +   ++ AM+RA++A       G+ C   K++A  ADC++LY +TI+QLNRT    
Sbjct: 64  LSEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGV 123

Query: 121 -----SDKYCSDFDIQTWLSSALTNLETCRAGFVDMNVKDYDVVFPFLARNNNVTQLISN 180
                + K C+DFD QTWLS+ALTN ETCR G  D+NV D+  + P ++ N  ++ LISN
Sbjct: 124 SPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDF--ITPIVS-NTKISHLISN 183

Query: 181 SLAMNNCSETEMG----GYDHGGFPTWVRGGDRRLLQAEGVPKADLVVAQDGSGNYRTVQ 240
            LA+N    T         +  GFPTW+   D+RLL+A    +A+LVVA+DGSG++ TVQ
Sbjct: 184 CLAVNGALLTAGNKGNTTANQKGFPTWLSRKDKRLLRA---VRANLVVAKDGSGHFNTVQ 243

Query: 241 AAVDGI---KLGSGRFVIHVKKGVYKENVVIGNKMKNLMLVGDGLKYTIITGSRSVGGGS 300
           AA+D     K+ SGRFVI+VK+G+Y+EN+ +     ++MLVGDG++ TIITG RSV GG 
Sbjct: 244 AAIDVAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGY 303

Query: 301 TTFNSATVAVTGEGFIARGITFRNTAGPENHQAVALRSGSDLSVFYRCGFEGYQDTLYVH 360
           TT+NSAT  + G  FIA+GITFRNTAGP   QAVALRS SDLS+FY+C  EGYQDTL VH
Sbjct: 304 TTYNSATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVH 363

Query: 361 SQRQFYRECYIYGTVDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISI 420
           SQRQFYRECYIYGTVDFIFGNAA V QNC+I  R+P+ GQ  V+TAQGR DP QNTGISI
Sbjct: 364 SQRQFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISI 423

Query: 421 HNSRVMASDDLKPALSSVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWTGN--FALN 480
           HNSR++ + DLKP + +V+TY+GRPW ++SRTV LQ+++D++V+P GW  W     F L+
Sbjct: 424 HNSRILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLD 483

Query: 481 TLYYGEYKNFGPASSTANRVKWKGYRVITSVTEASKFTVQNFIAGKSWLPPTGVPFTASL 522
           TL+Y EYKN GPASST  RV WKG+ V+   ++AS FTV  FIAG +WLP TG+PFT+ L
Sbjct: 484 TLFYAEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

BLAST of Tan0005553 vs. ExPASy Swiss-Prot
Match: Q9FHN4 (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3702 GN=PME60 PE=2 SV=1)

HSP 1 Score: 538.5 bits (1386), Expect = 8.4e-152
Identity = 284/534 (53.18%), Postives = 371/534 (69.48%), Query Frame = 0

Query: 5   LHFWILVIYSLSILLSMSPCFSRSPNAIDLWCSRTPYPDVCKHLF--KDAKLDPTKPSEL 64
           LH  ++++  L  L +++     S N ID WC +TPYP  CK  F        PT+ SE 
Sbjct: 14  LHLLLILLLCLRPLTTVAD--GNSTN-IDGWCDKTPYPYPCKRYFIKHSGFRLPTQISEF 73

Query: 65  KKTALKIAMERAVTAETLTKQLGEKCRSQKERAAWADCLELYQNTILQLNRTF------S 124
           +   ++ AM+RAV+A        + C   K++A  ADC+ LY +T++QLNRT       +
Sbjct: 74  RVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKT 133

Query: 125 DKYCSDFDIQTWLSSALTNLETCRAGFVDMNVKDYDVVFPFLARNNNVTQLISNSLAMN- 184
            + C+DFD QTWLS+ALTN ETCR G  D+NV D+      +  N  ++ LISN LA+N 
Sbjct: 134 GRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTP---IVSNTKISHLISNCLAVNG 193

Query: 185 ---NCSETEMGGYDHGGFPTWVRGGDRRLLQAEGVPKADLVVAQDGSGNYRTVQAAVDGI 244
                 + +    D  GFPTWV   +RRLLQ + V +A+LVVA+DGSG+++TVQAA+D  
Sbjct: 194 ALLTAGKNDSTTGDSKGFPTWVSRKERRLLQLQSV-RANLVVAKDGSGHFKTVQAAIDVA 253

Query: 245 ---KLGSGRFVIHVKKGVYKENVVIGNKMKNLMLVGDGLKYTIITGSRSVGGGSTTFNSA 304
              K+ SGRFVI+VK+G+Y+EN+ +     N+MLVGDG++YTIITG RSV GG TT++SA
Sbjct: 254 GRRKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSA 313

Query: 305 TVAVTGEGFIARGITFRNTAGPENHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFY 364
           T  + G  FIA+GI F+NTAGP   QAVALRS SDLS+FYRC  EGYQDTL VHSQRQFY
Sbjct: 314 TAGIEGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFY 373

Query: 365 RECYIYGTVDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVM 424
           RECYIYGTVDFIFGNAAVV QNC+I  R P+ GQ  V+TAQGRTD  QNTGISIHNS ++
Sbjct: 374 RECYIYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIII 433

Query: 425 ASDDLKPALSSVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWT--GNFALNTLYYGE 484
            + DLKP + SV+TY+GRPW  YSRTV L++++DS+V+P GW  WT    + L+TL+Y E
Sbjct: 434 PAPDLKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAE 493

Query: 485 YKNFGPASSTANRVKWKGYRVITSVTEASKFTVQNFIAGKSWLPPTGVPFTASL 522
           YKN GPASST  RV+WKG+ V++  ++AS F+V  FIAG +WLP +G+PFT+ L
Sbjct: 494 YKNIGPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFTSEL 540

BLAST of Tan0005553 vs. ExPASy Swiss-Prot
Match: Q9STY3 (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3702 GN=PME33 PE=2 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 1.5e-145
Identity = 265/514 (51.56%), Postives = 356/514 (69.26%), Query Frame = 0

Query: 35  WCSRTPYPDVCKHLFKDAKLD------PTKPSELKKTALKIAMERAVTAETLTKQLGEKC 94
           WC++TP+ + C + F+ +  +      P   SE  +  +K+A+++AV   + T + G  C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 95  RSQKERAAWADCLELYQNTILQLNRTF--------SDKYCSDFDIQTWLSSALTNLETCR 154
            + + +AAW+DC+ L+QNT+ QLNRT         SD  C+DFD QTWLS+A TN+ETCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 155 AGFVDMNVKDYDVVFPFLARNNNVTQLISNSLAMNNCSETEMGGYDHGG-------FPTW 214
           +G  D+NV D+  V P ++ N N++ LI N LA+N      M  +DH         FP+W
Sbjct: 207 SGSEDLNVSDF--VMPVIS-NKNLSDLIGNCLAVNG---VLMKQHDHTTTANHKEYFPSW 266

Query: 215 VRGGDRRLLQAEGVPKAD--LVVAQDGSGNYRTVQAAVD--GIKLGSGRFVIHVKKGVYK 274
           V   +RRLL +  + K+   LVVAQD SG++R++QAA++    +    RFVI+VKKGVY+
Sbjct: 267 VSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYR 326

Query: 275 ENVVIGNKMKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFRNTA 334
           EN+ +GN   N+MLVGDG + TIIT  RSV  G TT+NSAT    G+ F+A+ +TF NTA
Sbjct: 327 ENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINTA 386

Query: 335 GPENHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVVL 394
           GP   QAVA+RS SDLSVFYR G  G+QDTLY+HSQRQF+RECYI GT+DFIFGNAAVV 
Sbjct: 387 GPLRGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVVF 446

Query: 395 QNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPALSSVRTYLGRPW 454
           QNCMI  R+P+ GQ  ++TAQGR DP QNTGI+IH+SR++A+ DLKP + + +TYLGRPW
Sbjct: 447 QNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRPW 506

Query: 455 KEYSRTVYLQSFMDSLVNPAGWLEWT--GNFALNTLYYGEYKNFGPASSTANRVKWKGYR 514
           + YSR   +++++D+ ++P GW  W    NFALNT++YGEYKNFGP SST  RV+WKG+ 
Sbjct: 507 QAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGFH 566

Query: 515 VITSVTEASKFTVQNFIAGKSWLPPTGVPFTASL 522
            ITS   AS+FTV + IAG SWLP TGVPF + L
Sbjct: 567 AITSTAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

BLAST of Tan0005553 vs. NCBI nr
Match: XP_022996028.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 879.8 bits (2272), Expect = 1.2e-251
Identity = 443/523 (84.70%), Postives = 475/523 (90.82%), Query Frame = 0

Query: 1   MAEKLHFWILVIYSLSILLSMSPCFSRSPNAIDLWCSRTPYPDVCKHLFKDAKLDPTKPS 60
           MA+ LHF ILVI S +ILLS+SP FS +PNA DLWCS+TPYPDVCK  FK AK +P   +
Sbjct: 1   MAQNLHFPILVI-SFTILLSISPSFS-APNAKDLWCSQTPYPDVCKSFFK-AKFNPKNLA 60

Query: 61  ELKKTALKIAMERAVTAETLTKQLGEKCRSQKERAAWADCLELYQNTILQLNRTFSDKYC 120
           E KKTA KIAMERA+TAETLTK+  +KCR+ KE+ AWADCLELYQ+TIL LNRTFSD YC
Sbjct: 61  EFKKTAFKIAMERALTAETLTKRHAQKCRTPKEKGAWADCLELYQDTILHLNRTFSDNYC 120

Query: 121 SDFDIQTWLSSALTNLETCRAGFVDMNVKDYDVVFPFLARNNNVTQLISNSLAMNNCSET 180
           SDFDIQTWLSSALTNLETCRAGFVD+N+KDYDVVFPFL+R+NNVT+LI NSLAMNNCSET
Sbjct: 121 SDFDIQTWLSSALTNLETCRAGFVDLNIKDYDVVFPFLSRSNNVTKLIRNSLAMNNCSET 180

Query: 181 EMGGYDHGGFPTWVRGGDRRLLQAEGVPKADLVVAQDGSGNYRTVQAAVDGI--KLGSGR 240
           E G   HGGFPTWV+ G+RRLLQA  VPKADLVVA DGSGNY+TVQAAVD    + GSGR
Sbjct: 181 ETG--YHGGFPTWVKAGERRLLQA-AVPKADLVVALDGSGNYKTVQAAVDAAAKRKGSGR 240

Query: 241 FVIHVKKGVYKENVVIGNKMKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGF 300
           FVIHVKKGVYKENVVIGNK+KNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGF
Sbjct: 241 FVIHVKKGVYKENVVIGNKVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGF 300

Query: 301 IARGITFRNTAGPENHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTV 360
           IARGITFRNTAGPENHQAVALRSGSDLSVFYRC FEGYQDTLYVHSQRQFYRECYIYGTV
Sbjct: 301 IARGITFRNTAGPENHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECYIYGTV 360

Query: 361 DFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPAL 420
           DFIFGNAAVVLQNCMIYARKP+ GQQCVVTAQGR+DPNQNTGISIHNSRVMA+ DL+P L
Sbjct: 361 DFIFGNAAVVLQNCMIYARKPMSGQQCVVTAQGRSDPNQNTGISIHNSRVMATADLQPVL 420

Query: 421 SSVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWTGNFALNTLYYGEYKNFGPASSTA 480
           SSVRTYLGRPW+EYSRTVYLQ FMDSLVNPAGWLEW+G+FALNTLYYGE+KNFGP SSTA
Sbjct: 421 SSVRTYLGRPWREYSRTVYLQCFMDSLVNPAGWLEWSGDFALNTLYYGEFKNFGPGSSTA 480

Query: 481 NRVKWKGYRVITSVTEASKFTVQNFIAGKSWLPPTGVPFTASL 522
            RVKWKGYRVITS  EASKFTV +FIAGKSWLP TGVPFTA L
Sbjct: 481 KRVKWKGYRVITSAAEASKFTVGSFIAGKSWLPATGVPFTAGL 517

BLAST of Tan0005553 vs. NCBI nr
Match: XP_038875884.1 (pectinesterase 2-like [Benincasa hispida])

HSP 1 Score: 875.9 bits (2262), Expect = 1.7e-250
Identity = 441/525 (84.00%), Postives = 477/525 (90.86%), Query Frame = 0

Query: 1   MAEKLHFWILVIYSLSILLSMSPCFSRSP--NAIDLWCSRTPYPDVCKHLFKDAKLDPTK 60
           MA+KLHF I++I SL IL S+S CFS  P  NAIDLWCSR PYPDVCKH F + + DP K
Sbjct: 1   MAQKLHFSIILI-SLFILSSISLCFSTKPNNNAIDLWCSRMPYPDVCKHFF-NGQFDPKK 60

Query: 61  PSELKKTALKIAMERAVTAETLTKQLGEKCRSQKERAAWADCLELYQNTILQLNRTFSDK 120
            SE KKTALKIAM+RA+ AETLTK+LG+KCR++KERAAWADCLELYQ TIL LN+TFSD+
Sbjct: 61  LSEFKKTALKIAMDRAIRAETLTKELGKKCRNEKERAAWADCLELYQTTILHLNKTFSDE 120

Query: 121 YCSDFDIQTWLSSALTNLETCRAGFVDMNVKDYDVVFPFLARNNNVTQLISNSLAMNNCS 180
            CSDFDIQTWLSSALT+LETCRAGF+D+ +KDYDVVFPFLA NNNVT+LISNSLAMNNCS
Sbjct: 121 KCSDFDIQTWLSSALTDLETCRAGFIDLKIKDYDVVFPFLA-NNNVTKLISNSLAMNNCS 180

Query: 181 ETEMGGYDHGGFPTWVRGGDRRLLQAEGVPKADLVVAQDGSGNYRTVQAAVD--GIKLGS 240
           E+++G   + GFP WVR GDRRLLQA   PKADLVVAQDGSGNYRTVQAAVD  G + GS
Sbjct: 181 ESDVGYKSNEGFPKWVRRGDRRLLQA-AEPKADLVVAQDGSGNYRTVQAAVDAAGKRKGS 240

Query: 241 GRFVIHVKKGVYKENVVIGNKMKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGE 300
           GRFVIH+K+GVYKENVVI  K+KNLMLVGDGL+YTIITGSRSVGGGSTTFNSATVAVTGE
Sbjct: 241 GRFVIHIKRGVYKENVVI--KVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGE 300

Query: 301 GFIARGITFRNTAGPENHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYG 360
            FIARGITFRNTAGPENHQAVALRSGSDLSVF+RCGFEGYQDTLY HSQRQFYRECYIYG
Sbjct: 301 RFIARGITFRNTAGPENHQAVALRSGSDLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYG 360

Query: 361 TVDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKP 420
           TVDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMA+DDLKP
Sbjct: 361 TVDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKP 420

Query: 421 ALSSVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWTGNFALNTLYYGEYKNFGPASS 480
            + SVRTYLGRPWKEYSRTVYLQ+FMD+LVNPAGWLEW+GNFALNTLYYGE+KNFGP SS
Sbjct: 421 VVGSVRTYLGRPWKEYSRTVYLQNFMDNLVNPAGWLEWSGNFALNTLYYGEFKNFGPGSS 480

Query: 481 TANRVKWKGYRVITSVTEASKFTVQNFIAGKSWLPPTGVPFTASL 522
           TA RVKWKGYRVITS TEASKFTV NFIAG+SWLP TGVPFTA L
Sbjct: 481 TAKRVKWKGYRVITSATEASKFTVGNFIAGRSWLPGTGVPFTAGL 519

BLAST of Tan0005553 vs. NCBI nr
Match: XP_022957920.1 (pectinesterase-like [Cucurbita moschata])

HSP 1 Score: 872.1 bits (2252), Expect = 2.4e-249
Identity = 438/523 (83.75%), Postives = 474/523 (90.63%), Query Frame = 0

Query: 1   MAEKLHFWILVIYSLSILLSMSPCFSRSPNAIDLWCSRTPYPDVCKHLFKDAKLDPTKPS 60
           MA+  H  ILVI S+SILL++SP FS +PNA DLWCS+TPYPDVCK  F +AK +P   +
Sbjct: 1   MAQNRHVSILVI-SMSILLAISPSFS-APNAKDLWCSQTPYPDVCKSFF-NAKFNPKNLA 60

Query: 61  ELKKTALKIAMERAVTAETLTKQLGEKCRSQKERAAWADCLELYQNTILQLNRTFSDKYC 120
           E KKTA KIAMERA+TAE+LTK+  +KCR+ KE+ AWADCLELYQ+TILQLNRTFSD YC
Sbjct: 61  EFKKTAFKIAMERALTAESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYC 120

Query: 121 SDFDIQTWLSSALTNLETCRAGFVDMNVKDYDVVFPFLARNNNVTQLISNSLAMNNCSET 180
           SDFDIQTWLSSALTNLETCRAGFVD+N+KDYDVVFPFL R+NNVT+LI NSLAMNNCSET
Sbjct: 121 SDFDIQTWLSSALTNLETCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSET 180

Query: 181 EMGGYDHGGFPTWVRGGDRRLLQAEGVPKADLVVAQDGSGNYRTVQAAVDGI--KLGSGR 240
           E G   HGGFPTWV+ G+RRLLQA  VPKADLVVA DGSGNY+TVQAAVD    + GSGR
Sbjct: 181 ETG--YHGGFPTWVKAGERRLLQA-AVPKADLVVALDGSGNYKTVQAAVDAAAKRKGSGR 240

Query: 241 FVIHVKKGVYKENVVIGNKMKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGF 300
           FVIHVKKGVYKENVVIGNK+KNLMLVGDGLKYTIITGSRSV GGSTTFNSATVAVTGEGF
Sbjct: 241 FVIHVKKGVYKENVVIGNKVKNLMLVGDGLKYTIITGSRSVRGGSTTFNSATVAVTGEGF 300

Query: 301 IARGITFRNTAGPENHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTV 360
           IARGITFRNTAGP+NHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFY+ECYIYGTV
Sbjct: 301 IARGITFRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTV 360

Query: 361 DFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPAL 420
           DFIFGNAAVVLQNCMIYARKP+ GQQCVVTAQGR+DPNQNTGISIHNSRVMA+ DL+P L
Sbjct: 361 DFIFGNAAVVLQNCMIYARKPMSGQQCVVTAQGRSDPNQNTGISIHNSRVMATADLQPVL 420

Query: 421 SSVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWTGNFALNTLYYGEYKNFGPASSTA 480
           SSVRTYLGRPWKEYSRTVY+Q FMDSLVNPAGWLEW+G+FALNTLYYGE+KNFGP SSTA
Sbjct: 421 SSVRTYLGRPWKEYSRTVYMQCFMDSLVNPAGWLEWSGDFALNTLYYGEFKNFGPGSSTA 480

Query: 481 NRVKWKGYRVITSVTEASKFTVQNFIAGKSWLPPTGVPFTASL 522
            RVKWKGYRVITS  EASKFTV +FIAGKSWLP TGVPFTA L
Sbjct: 481 KRVKWKGYRVITSAAEASKFTVGSFIAGKSWLPATGVPFTAGL 517

BLAST of Tan0005553 vs. NCBI nr
Match: XP_023534348.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 870.5 bits (2248), Expect = 7.1e-249
Identity = 436/523 (83.37%), Postives = 473/523 (90.44%), Query Frame = 0

Query: 1   MAEKLHFWILVIYSLSILLSMSPCFSRSPNAIDLWCSRTPYPDVCKHLFKDAKLDPTKPS 60
           MA+  HF IL+I S+SILLS+SP  S +PNA +LWCS+TPYPD+CK  F +AK +P   +
Sbjct: 1   MAQNRHFSILLI-SMSILLSISPSLS-APNAKELWCSQTPYPDICKSFF-NAKFNPKNLA 60

Query: 61  ELKKTALKIAMERAVTAETLTKQLGEKCRSQKERAAWADCLELYQNTILQLNRTFSDKYC 120
           E KK A KIAMERA+TAE+LTK+  +KCR+ KE+ AWADCLELYQ+TILQLNRTFSD YC
Sbjct: 61  EFKKAAFKIAMERALTAESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYC 120

Query: 121 SDFDIQTWLSSALTNLETCRAGFVDMNVKDYDVVFPFLARNNNVTQLISNSLAMNNCSET 180
           SDFDIQTWLSSALTNL+TCRAGFVD+N+KDYDVVFPFL R+NNVT+LI NSLAMNNCSET
Sbjct: 121 SDFDIQTWLSSALTNLDTCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSET 180

Query: 181 EMGGYDHGGFPTWVRGGDRRLLQAEGVPKADLVVAQDGSGNYRTVQAAVDGI--KLGSGR 240
           E G   HGGFPTWV+ G+RRLLQA  VPK DLVVA DGSGNY+TVQAAVD    + GSGR
Sbjct: 181 ETG--YHGGFPTWVKAGERRLLQA-AVPKPDLVVALDGSGNYKTVQAAVDAAAKRKGSGR 240

Query: 241 FVIHVKKGVYKENVVIGNKMKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGF 300
           FVIHVKKGVYKENVVIGNK+KNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGF
Sbjct: 241 FVIHVKKGVYKENVVIGNKVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGF 300

Query: 301 IARGITFRNTAGPENHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTV 360
           IARGITFRNTAGP+NHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTV
Sbjct: 301 IARGITFRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTV 360

Query: 361 DFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPAL 420
           DFIFGNAAVVLQNCMIYARKP+ GQQCVVTAQGR+DPNQNTGISIHNSRVMA+ DL+P L
Sbjct: 361 DFIFGNAAVVLQNCMIYARKPMSGQQCVVTAQGRSDPNQNTGISIHNSRVMATADLQPVL 420

Query: 421 SSVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWTGNFALNTLYYGEYKNFGPASSTA 480
           SSVRTYLGRPWKEYSRTVYLQ FMDSLVNPAGWLEW+G+FALNTLYYGE+KNFGP SSTA
Sbjct: 421 SSVRTYLGRPWKEYSRTVYLQCFMDSLVNPAGWLEWSGDFALNTLYYGEFKNFGPGSSTA 480

Query: 481 NRVKWKGYRVITSVTEASKFTVQNFIAGKSWLPPTGVPFTASL 522
            RVKWKGYRVITS  EASKFTV +FIAGKSWLP TGVPFTA L
Sbjct: 481 KRVKWKGYRVITSAAEASKFTVGSFIAGKSWLPATGVPFTAGL 517

BLAST of Tan0005553 vs. NCBI nr
Match: KAG6605649.1 (Pectinesterase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 867.5 bits (2240), Expect = 6.0e-248
Identity = 431/509 (84.68%), Postives = 465/509 (91.36%), Query Frame = 0

Query: 15  LSILLSMSPCFSRSPNAIDLWCSRTPYPDVCKHLFKDAKLDPTKPSELKKTALKIAMERA 74
           +SILL++SP FS +PNA DLWCS+TPYPDVCK  F +AK +P   +E KKTA KIAMERA
Sbjct: 1   MSILLAISPSFS-APNAKDLWCSQTPYPDVCKSFF-NAKFNPKNLAEFKKTAFKIAMERA 60

Query: 75  VTAETLTKQLGEKCRSQKERAAWADCLELYQNTILQLNRTFSDKYCSDFDIQTWLSSALT 134
           +TAE+LTK+  +KCR+ KE+ AWADCLELYQ+TILQLNRTFSD YCSDFDIQTWLSSALT
Sbjct: 61  LTAESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYCSDFDIQTWLSSALT 120

Query: 135 NLETCRAGFVDMNVKDYDVVFPFLARNNNVTQLISNSLAMNNCSETEMGGYDHGGFPTWV 194
           NLETCRAGFVD+N+KDYDVVFPFL R+NNVT+LI NSLAMNNCSETE G   HGGFPTWV
Sbjct: 121 NLETCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSETETG--YHGGFPTWV 180

Query: 195 RGGDRRLLQAEGVPKADLVVAQDGSGNYRTVQAAVDGI--KLGSGRFVIHVKKGVYKENV 254
           + G+RRLLQA  VPKADLVVA DGSGNY+TVQAAVD    + GSGRFVIHVKKGVYKENV
Sbjct: 181 KAGERRLLQA-AVPKADLVVALDGSGNYKTVQAAVDAAAKRKGSGRFVIHVKKGVYKENV 240

Query: 255 VIGNKMKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFRNTAGPE 314
           VIGNK+KNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFRNTAGP+
Sbjct: 241 VIGNKVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFRNTAGPQ 300

Query: 315 NHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVVLQNC 374
           NHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVVLQNC
Sbjct: 301 NHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVVLQNC 360

Query: 375 MIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPALSSVRTYLGRPWKEY 434
           MIYARKP+ GQQC VTAQGR+DPNQNTGISIHNSRVMA+ DL+P LSSVRTYLGRPWKEY
Sbjct: 361 MIYARKPMSGQQCAVTAQGRSDPNQNTGISIHNSRVMATADLQPVLSSVRTYLGRPWKEY 420

Query: 435 SRTVYLQSFMDSLVNPAGWLEWTGNFALNTLYYGEYKNFGPASSTANRVKWKGYRVITSV 494
           SRTVY+Q FMDSLVNPAGWLEW+G+FALNTLYYGE+KNFGP SSTA RVKWKGYRVITS 
Sbjct: 421 SRTVYMQCFMDSLVNPAGWLEWSGDFALNTLYYGEFKNFGPGSSTAKRVKWKGYRVITSA 480

Query: 495 TEASKFTVQNFIAGKSWLPPTGVPFTASL 522
            EASKFTV +FIAGKSWLP TGVPFTA L
Sbjct: 481 AEASKFTVGSFIAGKSWLPATGVPFTAGL 504

BLAST of Tan0005553 vs. ExPASy TrEMBL
Match: A0A6J1K9P3 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111491356 PE=3 SV=1)

HSP 1 Score: 879.8 bits (2272), Expect = 5.7e-252
Identity = 443/523 (84.70%), Postives = 475/523 (90.82%), Query Frame = 0

Query: 1   MAEKLHFWILVIYSLSILLSMSPCFSRSPNAIDLWCSRTPYPDVCKHLFKDAKLDPTKPS 60
           MA+ LHF ILVI S +ILLS+SP FS +PNA DLWCS+TPYPDVCK  FK AK +P   +
Sbjct: 1   MAQNLHFPILVI-SFTILLSISPSFS-APNAKDLWCSQTPYPDVCKSFFK-AKFNPKNLA 60

Query: 61  ELKKTALKIAMERAVTAETLTKQLGEKCRSQKERAAWADCLELYQNTILQLNRTFSDKYC 120
           E KKTA KIAMERA+TAETLTK+  +KCR+ KE+ AWADCLELYQ+TIL LNRTFSD YC
Sbjct: 61  EFKKTAFKIAMERALTAETLTKRHAQKCRTPKEKGAWADCLELYQDTILHLNRTFSDNYC 120

Query: 121 SDFDIQTWLSSALTNLETCRAGFVDMNVKDYDVVFPFLARNNNVTQLISNSLAMNNCSET 180
           SDFDIQTWLSSALTNLETCRAGFVD+N+KDYDVVFPFL+R+NNVT+LI NSLAMNNCSET
Sbjct: 121 SDFDIQTWLSSALTNLETCRAGFVDLNIKDYDVVFPFLSRSNNVTKLIRNSLAMNNCSET 180

Query: 181 EMGGYDHGGFPTWVRGGDRRLLQAEGVPKADLVVAQDGSGNYRTVQAAVDGI--KLGSGR 240
           E G   HGGFPTWV+ G+RRLLQA  VPKADLVVA DGSGNY+TVQAAVD    + GSGR
Sbjct: 181 ETG--YHGGFPTWVKAGERRLLQA-AVPKADLVVALDGSGNYKTVQAAVDAAAKRKGSGR 240

Query: 241 FVIHVKKGVYKENVVIGNKMKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGF 300
           FVIHVKKGVYKENVVIGNK+KNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGF
Sbjct: 241 FVIHVKKGVYKENVVIGNKVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGF 300

Query: 301 IARGITFRNTAGPENHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTV 360
           IARGITFRNTAGPENHQAVALRSGSDLSVFYRC FEGYQDTLYVHSQRQFYRECYIYGTV
Sbjct: 301 IARGITFRNTAGPENHQAVALRSGSDLSVFYRCSFEGYQDTLYVHSQRQFYRECYIYGTV 360

Query: 361 DFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPAL 420
           DFIFGNAAVVLQNCMIYARKP+ GQQCVVTAQGR+DPNQNTGISIHNSRVMA+ DL+P L
Sbjct: 361 DFIFGNAAVVLQNCMIYARKPMSGQQCVVTAQGRSDPNQNTGISIHNSRVMATADLQPVL 420

Query: 421 SSVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWTGNFALNTLYYGEYKNFGPASSTA 480
           SSVRTYLGRPW+EYSRTVYLQ FMDSLVNPAGWLEW+G+FALNTLYYGE+KNFGP SSTA
Sbjct: 421 SSVRTYLGRPWREYSRTVYLQCFMDSLVNPAGWLEWSGDFALNTLYYGEFKNFGPGSSTA 480

Query: 481 NRVKWKGYRVITSVTEASKFTVQNFIAGKSWLPPTGVPFTASL 522
            RVKWKGYRVITS  EASKFTV +FIAGKSWLP TGVPFTA L
Sbjct: 481 KRVKWKGYRVITSAAEASKFTVGSFIAGKSWLPATGVPFTAGL 517

BLAST of Tan0005553 vs. ExPASy TrEMBL
Match: A0A6J1H1Z9 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459307 PE=3 SV=1)

HSP 1 Score: 872.1 bits (2252), Expect = 1.2e-249
Identity = 438/523 (83.75%), Postives = 474/523 (90.63%), Query Frame = 0

Query: 1   MAEKLHFWILVIYSLSILLSMSPCFSRSPNAIDLWCSRTPYPDVCKHLFKDAKLDPTKPS 60
           MA+  H  ILVI S+SILL++SP FS +PNA DLWCS+TPYPDVCK  F +AK +P   +
Sbjct: 1   MAQNRHVSILVI-SMSILLAISPSFS-APNAKDLWCSQTPYPDVCKSFF-NAKFNPKNLA 60

Query: 61  ELKKTALKIAMERAVTAETLTKQLGEKCRSQKERAAWADCLELYQNTILQLNRTFSDKYC 120
           E KKTA KIAMERA+TAE+LTK+  +KCR+ KE+ AWADCLELYQ+TILQLNRTFSD YC
Sbjct: 61  EFKKTAFKIAMERALTAESLTKRHAQKCRTPKEKGAWADCLELYQDTILQLNRTFSDNYC 120

Query: 121 SDFDIQTWLSSALTNLETCRAGFVDMNVKDYDVVFPFLARNNNVTQLISNSLAMNNCSET 180
           SDFDIQTWLSSALTNLETCRAGFVD+N+KDYDVVFPFL R+NNVT+LI NSLAMNNCSET
Sbjct: 121 SDFDIQTWLSSALTNLETCRAGFVDLNIKDYDVVFPFLGRSNNVTKLIRNSLAMNNCSET 180

Query: 181 EMGGYDHGGFPTWVRGGDRRLLQAEGVPKADLVVAQDGSGNYRTVQAAVDGI--KLGSGR 240
           E G   HGGFPTWV+ G+RRLLQA  VPKADLVVA DGSGNY+TVQAAVD    + GSGR
Sbjct: 181 ETG--YHGGFPTWVKAGERRLLQA-AVPKADLVVALDGSGNYKTVQAAVDAAAKRKGSGR 240

Query: 241 FVIHVKKGVYKENVVIGNKMKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGF 300
           FVIHVKKGVYKENVVIGNK+KNLMLVGDGLKYTIITGSRSV GGSTTFNSATVAVTGEGF
Sbjct: 241 FVIHVKKGVYKENVVIGNKVKNLMLVGDGLKYTIITGSRSVRGGSTTFNSATVAVTGEGF 300

Query: 301 IARGITFRNTAGPENHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTV 360
           IARGITFRNTAGP+NHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFY+ECYIYGTV
Sbjct: 301 IARGITFRNTAGPQNHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYKECYIYGTV 360

Query: 361 DFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPAL 420
           DFIFGNAAVVLQNCMIYARKP+ GQQCVVTAQGR+DPNQNTGISIHNSRVMA+ DL+P L
Sbjct: 361 DFIFGNAAVVLQNCMIYARKPMSGQQCVVTAQGRSDPNQNTGISIHNSRVMATADLQPVL 420

Query: 421 SSVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWTGNFALNTLYYGEYKNFGPASSTA 480
           SSVRTYLGRPWKEYSRTVY+Q FMDSLVNPAGWLEW+G+FALNTLYYGE+KNFGP SSTA
Sbjct: 421 SSVRTYLGRPWKEYSRTVYMQCFMDSLVNPAGWLEWSGDFALNTLYYGEFKNFGPGSSTA 480

Query: 481 NRVKWKGYRVITSVTEASKFTVQNFIAGKSWLPPTGVPFTASL 522
            RVKWKGYRVITS  EASKFTV +FIAGKSWLP TGVPFTA L
Sbjct: 481 KRVKWKGYRVITSAAEASKFTVGSFIAGKSWLPATGVPFTAGL 517

BLAST of Tan0005553 vs. ExPASy TrEMBL
Match: A0A1S3ASJ4 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103482536 PE=3 SV=1)

HSP 1 Score: 850.1 bits (2195), Expect = 4.8e-243
Identity = 428/525 (81.52%), Postives = 467/525 (88.95%), Query Frame = 0

Query: 1   MAEKLHFWILVIYSLSILLSMSPCFSRSPN--AIDLWCSRTPYPDVCKHLFKDAKLDPTK 60
           MA+KLHF IL ++SL IL S S  FS   N  AIDLWCSRTPYPDVCK+ F D + DP  
Sbjct: 1   MAQKLHFSIL-LFSLFILSSTSLPFSTKTNKKAIDLWCSRTPYPDVCKNFFNDGQFDPKN 60

Query: 61  PSELKKTALKIAMERAVTAETLTKQLGEKCRSQKERAAWADCLELYQNTILQLNRTFSDK 120
             +++KTALKIAMERA+  ETLTK LG+KCR++KERAAWADCLELYQ TIL LN+TFSD 
Sbjct: 61  LLDIQKTALKIAMERAIRTETLTKALGQKCRNKKERAAWADCLELYQTTILHLNKTFSD- 120

Query: 121 YCSDFDIQTWLSSALTNLETCRAGFVDMNVKDYDVVFPFLARNNNVTQLISNSLAMNNCS 180
           +CS+FDIQTWLSSALTNL+TCRAGFVD+ +KDY VVFPFLA NNN+T+LISNSLAMNNCS
Sbjct: 121 HCSNFDIQTWLSSALTNLDTCRAGFVDLGIKDYGVVFPFLA-NNNITKLISNSLAMNNCS 180

Query: 181 ETEMGGYDHGGFPTWVRGGDRRLLQAEGVPKADLVVAQDGSGNYRTVQAAVD--GIKLGS 240
           E++ G     GFP WVRGGDRRLLQA   PKADLVVAQDGSGNY+TVQAAVD  G + GS
Sbjct: 181 ESDEGNTSDEGFPKWVRGGDRRLLQA-AEPKADLVVAQDGSGNYKTVQAAVDAAGKRKGS 240

Query: 241 GRFVIHVKKGVYKENVVIGNKMKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGE 300
           GRFVI +KKGVYKENVVI  K+KNLMLVGDGL+YTIITGSRSVGGGSTTFNSATVAVTGE
Sbjct: 241 GRFVIRIKKGVYKENVVI--KVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGE 300

Query: 301 GFIARGITFRNTAGPENHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYG 360
            FIARGITFRNTAGP+NHQAVALRSG+DLSVF+RCGFEGYQDTLY HSQRQFYRECYIYG
Sbjct: 301 RFIARGITFRNTAGPQNHQAVALRSGADLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYG 360

Query: 361 TVDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKP 420
           T+DFIFGNAAVVLQNCMIYARKP+ GQQCVVTAQGRTDPNQNTGISIHNSRVMA+DDLKP
Sbjct: 361 TIDFIFGNAAVVLQNCMIYARKPMQGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKP 420

Query: 421 ALSSVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWTGNFALNTLYYGEYKNFGPASS 480
            + SVRTYLGRPWKEYSRTVYLQSFM+SLVNPAGWLEW+GNFALNTLYYGE+KN+GP SS
Sbjct: 421 VIKSVRTYLGRPWKEYSRTVYLQSFMESLVNPAGWLEWSGNFALNTLYYGEFKNYGPGSS 480

Query: 481 TANRVKWKGYRVITSVTEASKFTVQNFIAGKSWLPPTGVPFTASL 522
           TANRVKWKGYRVITS  EA+KFTV NFIAG SWLP TGVPFTA L
Sbjct: 481 TANRVKWKGYRVITSANEAAKFTVGNFIAGNSWLPGTGVPFTAGL 519

BLAST of Tan0005553 vs. ExPASy TrEMBL
Match: A0A0A0KPG9 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G167190 PE=3 SV=1)

HSP 1 Score: 847.0 bits (2187), Expect = 4.1e-242
Identity = 426/525 (81.14%), Postives = 464/525 (88.38%), Query Frame = 0

Query: 1   MAEKLHFWILVIYSLSILLSMSPCFSRSPN--AIDLWCSRTPYPDVCKHLFKDAKLDPTK 60
           MA+KLHF I +++S+ IL S S  FS   N  AI+LWCSRTPYPDVCKH F + + DP  
Sbjct: 1   MAQKLHFSI-ILFSMFILSSSSLPFSTKTNNKAIELWCSRTPYPDVCKHFFNNGEFDPRN 60

Query: 61  PSELKKTALKIAMERAVTAETLTKQLGEKCRSQKERAAWADCLELYQNTILQLNRTFSDK 120
             ++KK ALKIAMERA+  ETLTK LG+KCR++KERAAWADCLELYQ TIL LN+TFSDK
Sbjct: 61  LLDIKKAALKIAMERAMKTETLTKALGQKCRNKKERAAWADCLELYQTTILHLNKTFSDK 120

Query: 121 YCSDFDIQTWLSSALTNLETCRAGFVDMNVKDYDVVFPFLARNNNVTQLISNSLAMNNCS 180
            CS+FDIQTWLSSALTNL TCRAGFVD+ +KDY VVFPFL  NNN+T+LISNSLAMNNCS
Sbjct: 121 NCSNFDIQTWLSSALTNLHTCRAGFVDLGIKDYGVVFPFL-ENNNITKLISNSLAMNNCS 180

Query: 181 ETEMGGYDHGGFPTWVRGGDRRLLQAEGVPKADLVVAQDGSGNYRTVQAAVD--GIKLGS 240
           E++ G     GFP W+ GGDRRLLQA   PKADLVVAQDGSGNY+TVQAAVD  G + GS
Sbjct: 181 ESDEGNTSDEGFPKWLHGGDRRLLQA-AEPKADLVVAQDGSGNYKTVQAAVDAAGKRKGS 240

Query: 241 GRFVIHVKKGVYKENVVIGNKMKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGE 300
           GRFVI VKKGVYKENVVI  K+KNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGE
Sbjct: 241 GRFVIRVKKGVYKENVVI--KVKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGE 300

Query: 301 GFIARGITFRNTAGPENHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYG 360
            FIARGITFRNTAGP+NHQAVALRSG+DLSVF+RCGFEGYQDTLYVHSQRQFYRECYIYG
Sbjct: 301 RFIARGITFRNTAGPQNHQAVALRSGADLSVFFRCGFEGYQDTLYVHSQRQFYRECYIYG 360

Query: 361 TVDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKP 420
           TVDFIFGN+AVVLQNCMIYARKP+ GQQCVVTAQGRTDPNQNTGISIHNSRVMA+DDLKP
Sbjct: 361 TVDFIFGNSAVVLQNCMIYARKPMQGQQCVVTAQGRTDPNQNTGISIHNSRVMATDDLKP 420

Query: 421 ALSSVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWTGNFALNTLYYGEYKNFGPASS 480
            + SVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEW+GNFALNTLYYGE+KN GP SS
Sbjct: 421 VIKSVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWSGNFALNTLYYGEFKNSGPGSS 480

Query: 481 TANRVKWKGYRVITSVTEASKFTVQNFIAGKSWLPPTGVPFTASL 522
           TANRVKWKGYRVITS +EA+KFTV +FIAG SWLP TGVPFTA L
Sbjct: 481 TANRVKWKGYRVITSASEAAKFTVGSFIAGNSWLPGTGVPFTAGL 520

BLAST of Tan0005553 vs. ExPASy TrEMBL
Match: A0A5A7ULB7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00120 PE=3 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 1.5e-220
Identity = 384/453 (84.77%), Postives = 415/453 (91.61%), Query Frame = 0

Query: 71  MERAVTAETLTKQLGEKCRSQKERAAWADCLELYQNTILQLNRTFSDKYCSDFDIQTWLS 130
           MERA+  ETLTK LG+KCR++KERAAWADCLELYQ TIL LN+TFSD +CS+FDIQTWLS
Sbjct: 1   MERAIRTETLTKALGQKCRNKKERAAWADCLELYQTTILHLNKTFSD-HCSNFDIQTWLS 60

Query: 131 SALTNLETCRAGFVDMNVKDYDVVFPFLARNNNVTQLISNSLAMNNCSETEMGGYDHGGF 190
           SALTNL+TCRAGFVD+ +KDY VVFPFLA NNN+T+LISNSLAMNNCSE++ G     GF
Sbjct: 61  SALTNLDTCRAGFVDLGIKDYGVVFPFLA-NNNITKLISNSLAMNNCSESDEGNTSDEGF 120

Query: 191 PTWVRGGDRRLLQAEGVPKADLVVAQDGSGNYRTVQAAVD--GIKLGSGRFVIHVKKGVY 250
           P WVRGGDRRLLQA   PKADLVVAQDGSGNY+TVQAAVD  G + GSGRFVI +KKGVY
Sbjct: 121 PKWVRGGDRRLLQA-AEPKADLVVAQDGSGNYKTVQAAVDAAGKRKGSGRFVIRIKKGVY 180

Query: 251 KENVVIGNKMKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFRNT 310
           KENVVI  K+KNLMLVGDGL+YTIITGSRSVGGGSTTFNSATVAVTGE FIARGITFRNT
Sbjct: 181 KENVVI--KVKNLMLVGDGLRYTIITGSRSVGGGSTTFNSATVAVTGERFIARGITFRNT 240

Query: 311 AGPENHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVV 370
           AGP+NHQAVALRSG+DLSVF+RCGFEGYQDTLY HSQRQFYRECYIYGT+DFIFGNAAVV
Sbjct: 241 AGPQNHQAVALRSGADLSVFFRCGFEGYQDTLYAHSQRQFYRECYIYGTIDFIFGNAAVV 300

Query: 371 LQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPALSSVRTYLGRP 430
           LQNCMIYARKP+ GQQCVVTAQGRTDPNQNTGISIHNSRVMA+DDLKP + SVRTYLGRP
Sbjct: 301 LQNCMIYARKPMQGQQCVVTAQGRTDPNQNTGISIHNSRVMAADDLKPVIKSVRTYLGRP 360

Query: 431 WKEYSRTVYLQSFMDSLVNPAGWLEWTGNFALNTLYYGEYKNFGPASSTANRVKWKGYRV 490
           WKEYSRTVYLQSFM+SLVNPAGWLEW+GNFALNTLYYGE+KN+GP SSTANRVKWKGYRV
Sbjct: 361 WKEYSRTVYLQSFMESLVNPAGWLEWSGNFALNTLYYGEFKNYGPGSSTANRVKWKGYRV 420

Query: 491 ITSVTEASKFTVQNFIAGKSWLPPTGVPFTASL 522
           ITS  EA+KFTV NFIAG SWLP TGVPFTA L
Sbjct: 421 ITSANEAAKFTVGNFIAGNSWLPGTGVPFTAGL 448

BLAST of Tan0005553 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 565.5 bits (1456), Expect = 4.5e-161
Identity = 288/515 (55.92%), Postives = 380/515 (73.79%), Query Frame = 0

Query: 10  LVIYSLSILLSMSPCFSRSPNAIDLWCSRTPYPDVCKH-LFKDAKLDPTK-PSELKKTAL 69
           ++ + +  +L  S     +   +  WCS+TP P  C++ L  ++  +P K  SE  K ++
Sbjct: 8   IINFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISM 67

Query: 70  KIAMERAVTAETLTKQLGEKCRSQKERAAWADCLELYQNTILQLNRTFSDKY-CSDFDIQ 129
           K+ ++RA+ A+T    LG KCR  +E+AAW DC++LY  T+ ++N T      CS  D Q
Sbjct: 68  KLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQ 127

Query: 130 TWLSSALTNLETCRAGFVDMNVKDYDVVFPFLARNNNVTQLISNSLAMNNCSETEMGGYD 189
           TWLS+ALTNL+TCRAGF+++ V   D+V P +  +NNV+ L+ N+LA+N          +
Sbjct: 128 TWLSTALTNLDTCRAGFLELGVT--DIVLPLM--SNNVSNLLCNTLAINKV-PFNYTPPE 187

Query: 190 HGGFPTWVRGGDRRLLQAEGVPKADLVVAQDGSGNYRTVQAAVDGIKLGSGRFVIHVKKG 249
             GFP+WV+ GDR+LLQ+   PK + VVA+DGSGN++T++ A+D    GSGRFVI+VK+G
Sbjct: 188 KDGFPSWVKPGDRKLLQS-STPKDNAVVAKDGSGNFKTIKEAIDAAS-GSGRFVIYVKQG 247

Query: 250 VYKENVVIGNKMKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFR 309
           VY EN+ I  + KN+ML GDG+  TIITGS+SVGGG+TTFNSATVA  G+GFIARGITFR
Sbjct: 248 VYSENLEI--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFR 307

Query: 310 NTAGPENHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAA 369
           NTAG  N QAVALRSGSDLSVFY+C FE YQDTLYVHS RQFYR+C +YGTVDFIFGNAA
Sbjct: 308 NTAGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAA 367

Query: 370 VVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPALSSVRTYLG 429
            VLQNC I+AR+P   +   +TAQGR+DPNQNTGI IHNSRV A+ DL+P L S +TYLG
Sbjct: 368 AVLQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLG 427

Query: 430 RPWKEYSRTVYLQSFMDSLVNPAGWLEWTGNFALNTLYYGEYKNFGPASSTANRVKWKGY 489
           RPW++YSRTV++++ +DSL++P GWLEW GNFAL TL+Y E++N GP +ST+ RV W G+
Sbjct: 428 RPWRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGF 487

Query: 490 RVITSVTEASKFTVQNFIAGKSWLPPTGVPFTASL 522
           RV+ S +EASKFTV  F+AG SW+ P+ VPFT+ L
Sbjct: 488 RVLGSASEASKFTVGTFLAGGSWI-PSSVPFTSGL 511

BLAST of Tan0005553 vs. TAIR 10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 545.4 bits (1404), Expect = 4.9e-155
Identity = 287/540 (53.15%), Postives = 374/540 (69.26%), Query Frame = 0

Query: 1   MAEKLHFWILVIYSLSILLSMSPCFSRSPNAIDLWCSRTPYPDVCKHLFKDAK--LDPTK 60
           M +KL    L +  L +LL + P  + +      WC +TPYPD CK  FK+      PT+
Sbjct: 4   MMQKLSILFLHLI-LLVLLCVHPLTTVADRNSTDWCDKTPYPDPCKCYFKNHNGFQQPTQ 63

Query: 61  PSELKKTALKIAMERAVTAETLTKQLGEKCRSQKERAAWADCLELYQNTILQLNRTF--- 120
            SE +   ++ AM+RA++A       G+ C   K++A  ADC++LY +TI+QLNRT    
Sbjct: 64  LSEFRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGV 123

Query: 121 -----SDKYCSDFDIQTWLSSALTNLETCRAGFVDMNVKDYDVVFPFLARNNNVTQLISN 180
                + K C+DFD QTWLS+ALTN ETCR G  D+NV D+  + P ++ N  ++ LISN
Sbjct: 124 SPKAGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDF--ITPIVS-NTKISHLISN 183

Query: 181 SLAMNNCSETEMG----GYDHGGFPTWVRGGDRRLLQAEGVPKADLVVAQDGSGNYRTVQ 240
            LA+N    T         +  GFPTW+   D+RLL+A    +A+LVVA+DGSG++ TVQ
Sbjct: 184 CLAVNGALLTAGNKGNTTANQKGFPTWLSRKDKRLLRA---VRANLVVAKDGSGHFNTVQ 243

Query: 241 AAVDGI---KLGSGRFVIHVKKGVYKENVVIGNKMKNLMLVGDGLKYTIITGSRSVGGGS 300
           AA+D     K+ SGRFVI+VK+G+Y+EN+ +     ++MLVGDG++ TIITG RSV GG 
Sbjct: 244 AAIDVAGRRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGY 303

Query: 301 TTFNSATVAVTGEGFIARGITFRNTAGPENHQAVALRSGSDLSVFYRCGFEGYQDTLYVH 360
           TT+NSAT  + G  FIA+GITFRNTAGP   QAVALRS SDLS+FY+C  EGYQDTL VH
Sbjct: 304 TTYNSATAGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVH 363

Query: 361 SQRQFYRECYIYGTVDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISI 420
           SQRQFYRECYIYGTVDFIFGNAA V QNC+I  R+P+ GQ  V+TAQGR DP QNTGISI
Sbjct: 364 SQRQFYRECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISI 423

Query: 421 HNSRVMASDDLKPALSSVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWTGN--FALN 480
           HNSR++ + DLKP + +V+TY+GRPW ++SRTV LQ+++D++V+P GW  W     F L+
Sbjct: 424 HNSRILPAPDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLD 483

Query: 481 TLYYGEYKNFGPASSTANRVKWKGYRVITSVTEASKFTVQNFIAGKSWLPPTGVPFTASL 522
           TL+Y EYKN GPASST  RV WKG+ V+   ++AS FTV  FIAG +WLP TG+PFT+ L
Sbjct: 484 TLFYAEYKNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

BLAST of Tan0005553 vs. TAIR 10
Match: AT5G51500.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 538.5 bits (1386), Expect = 6.0e-153
Identity = 284/534 (53.18%), Postives = 371/534 (69.48%), Query Frame = 0

Query: 5   LHFWILVIYSLSILLSMSPCFSRSPNAIDLWCSRTPYPDVCKHLF--KDAKLDPTKPSEL 64
           LH  ++++  L  L +++     S N ID WC +TPYP  CK  F        PT+ SE 
Sbjct: 14  LHLLLILLLCLRPLTTVAD--GNSTN-IDGWCDKTPYPYPCKRYFIKHSGFRLPTQISEF 73

Query: 65  KKTALKIAMERAVTAETLTKQLGEKCRSQKERAAWADCLELYQNTILQLNRTF------S 124
           +   ++ AM+RAV+A        + C   K++A  ADC+ LY +T++QLNRT       +
Sbjct: 74  RVLLVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKT 133

Query: 125 DKYCSDFDIQTWLSSALTNLETCRAGFVDMNVKDYDVVFPFLARNNNVTQLISNSLAMN- 184
            + C+DFD QTWLS+ALTN ETCR G  D+NV D+      +  N  ++ LISN LA+N 
Sbjct: 134 GRRCTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTP---IVSNTKISHLISNCLAVNG 193

Query: 185 ---NCSETEMGGYDHGGFPTWVRGGDRRLLQAEGVPKADLVVAQDGSGNYRTVQAAVDGI 244
                 + +    D  GFPTWV   +RRLLQ + V +A+LVVA+DGSG+++TVQAA+D  
Sbjct: 194 ALLTAGKNDSTTGDSKGFPTWVSRKERRLLQLQSV-RANLVVAKDGSGHFKTVQAAIDVA 253

Query: 245 ---KLGSGRFVIHVKKGVYKENVVIGNKMKNLMLVGDGLKYTIITGSRSVGGGSTTFNSA 304
              K+ SGRFVI+VK+G+Y+EN+ +     N+MLVGDG++YTIITG RSV GG TT++SA
Sbjct: 254 GRRKVTSGRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSA 313

Query: 305 TVAVTGEGFIARGITFRNTAGPENHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFY 364
           T  + G  FIA+GI F+NTAGP   QAVALRS SDLS+FYRC  EGYQDTL VHSQRQFY
Sbjct: 314 TAGIEGLHFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFY 373

Query: 365 RECYIYGTVDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVM 424
           RECYIYGTVDFIFGNAAVV QNC+I  R P+ GQ  V+TAQGRTD  QNTGISIHNS ++
Sbjct: 374 RECYIYGTVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIII 433

Query: 425 ASDDLKPALSSVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWT--GNFALNTLYYGE 484
            + DLKP + SV+TY+GRPW  YSRTV L++++DS+V+P GW  WT    + L+TL+Y E
Sbjct: 434 PAPDLKPVVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAE 493

Query: 485 YKNFGPASSTANRVKWKGYRVITSVTEASKFTVQNFIAGKSWLPPTGVPFTASL 522
           YKN GPASST  RV+WKG+ V++  ++AS F+V  FIAG +WLP +G+PFT+ L
Sbjct: 494 YKNIGPASSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPFTSEL 540

BLAST of Tan0005553 vs. TAIR 10
Match: AT3G47400.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 517.7 bits (1332), Expect = 1.1e-146
Identity = 265/514 (51.56%), Postives = 356/514 (69.26%), Query Frame = 0

Query: 35  WCSRTPYPDVCKHLFKDAKLD------PTKPSELKKTALKIAMERAVTAETLTKQLGEKC 94
           WC++TP+ + C + F+ +  +      P   SE  +  +K+A+++AV   + T + G  C
Sbjct: 87  WCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTVKFGPSC 146

Query: 95  RSQKERAAWADCLELYQNTILQLNRTF--------SDKYCSDFDIQTWLSSALTNLETCR 154
            + + +AAW+DC+ L+QNT+ QLNRT         SD  C+DFD QTWLS+A TN+ETCR
Sbjct: 147 TNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQTNIETCR 206

Query: 155 AGFVDMNVKDYDVVFPFLARNNNVTQLISNSLAMNNCSETEMGGYDHGG-------FPTW 214
           +G  D+NV D+  V P ++ N N++ LI N LA+N      M  +DH         FP+W
Sbjct: 207 SGSEDLNVSDF--VMPVIS-NKNLSDLIGNCLAVNG---VLMKQHDHTTTANHKEYFPSW 266

Query: 215 VRGGDRRLLQAEGVPKAD--LVVAQDGSGNYRTVQAAVD--GIKLGSGRFVIHVKKGVYK 274
           V   +RRLL +  + K+   LVVAQD SG++R++QAA++    +    RFVI+VKKGVY+
Sbjct: 267 VSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYR 326

Query: 275 ENVVIGNKMKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVTGEGFIARGITFRNTA 334
           EN+ +GN   N+MLVGDG + TIIT  RSV  G TT+NSAT    G+ F+A+ +TF NTA
Sbjct: 327 ENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINTA 386

Query: 335 GPENHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYIYGTVDFIFGNAAVVL 394
           GP   QAVA+RS SDLSVFYR G  G+QDTLY+HSQRQF+RECYI GT+DFIFGNAAVV 
Sbjct: 387 GPLRGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVVF 446

Query: 395 QNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDLKPALSSVRTYLGRPW 454
           QNCMI  R+P+ GQ  ++TAQGR DP QNTGI+IH+SR++A+ DLKP + + +TYLGRPW
Sbjct: 447 QNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRPW 506

Query: 455 KEYSRTVYLQSFMDSLVNPAGWLEWT--GNFALNTLYYGEYKNFGPASSTANRVKWKGYR 514
           + YSR   +++++D+ ++P GW  W    NFALNT++YGEYKNFGP SST  RV+WKG+ 
Sbjct: 507 QAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGFH 566

Query: 515 VITSVTEASKFTVQNFIAGKSWLPPTGVPFTASL 522
            ITS   AS+FTV + IAG SWLP TGVPF + L
Sbjct: 567 AITSTAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

BLAST of Tan0005553 vs. TAIR 10
Match: AT1G23200.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 449.1 bits (1154), Expect = 4.7e-126
Identity = 251/527 (47.63%), Postives = 328/527 (62.24%), Query Frame = 0

Query: 36  CSRTPYPDVCKHLFKDAKL----DPTKPSELKKTALKIAMERAVTAETLTKQLGEKCRSQ 95
           C +TPYP VC H   ++ L    D T         +   M++AV    L   L +     
Sbjct: 38  CKQTPYPSVCDHHMSNSPLKTLDDQTDGFTFHDLVVSSTMDQAVQLHRLVSSLKQHHSLH 97

Query: 96  KE-RAAWADCLELYQNTILQLN--RTFSDKYCSDFDIQTWLSSALTNLETCRAGFVDMNV 155
           K   +A  DCLELY++TI QLN  R    +Y S  D QT LS+A+ N +TCR GF D  +
Sbjct: 98  KHATSALFDCLELYEDTIDQLNHSRRSYGQYSSPHDRQTSLSAAIANQDTCRNGFRDFKL 157

Query: 156 -KDYDVVFPFLARNNNVTQLISNSLAMNNCS---------------------ETEMGGYD 215
              Y   FP +  + N+T+ ISNSLA+   +                      +  GG  
Sbjct: 158 TSSYSKYFP-VQFHRNLTKSISNSLAVTKAAAEAEAVAEKYPSTGFTKFSKQRSSAGGGS 217

Query: 216 H--------GGFPTWVRGGDRRLLQ-AEGVPKADLVVAQDGSGNYRTVQAAVDG---IKL 275
           H          FP+W    DR+LL+ ++   KADLVVA+DGSG+Y ++Q AV+    +  
Sbjct: 218 HRRLLLFSDEKFPSWFPLSDRKLLEDSKTTAKADLVVAKDGSGHYTSIQQAVNAAAKLPR 277

Query: 276 GSGRFVIHVKKGVYKENVVIGNKMKNLMLVGDGLKYTIITGSRSVGGGSTTFNSATVAVT 335
            + R VI+VK GVY+ENVVI   +KN+M++GDG+  TI+TG+R+V  G+TTF SAT AV+
Sbjct: 278 RNQRLVIYVKAGVYRENVVIKKSIKNVMVIGDGIDSTIVTGNRNVQDGTTTFRSATFAVS 337

Query: 336 GEGFIARGITFRNTAGPENHQAVALRSGSDLSVFYRCGFEGYQDTLYVHSQRQFYRECYI 395
           G GFIA+GITF NTAGPE HQAVALRS SD SVFY C F+GYQDTLY+HS RQF R C I
Sbjct: 338 GNGFIAQGITFENTAGPEKHQAVALRSSSDFSVFYACSFKGYQDTLYLHSSRQFLRNCNI 397

Query: 396 YGTVDFIFGNAAVVLQNCMIYARKPIPGQQCVVTAQGRTDPNQNTGISIHNSRVMASDDL 455
           YGTVDFIFG+A  +LQNC IYARKP+ GQ+  +TAQ R +P++ TG  I +S V  + + 
Sbjct: 398 YGTVDFIFGDATAILQNCNIYARKPMSGQKNTITAQSRKEPDETTGFVIQSSTVATASE- 457

Query: 456 KPALSSVRTYLGRPWKEYSRTVYLQSFMDSLVNPAGWLEWTGNFALNTLYYGEYKNFGPA 515
                   TYLGRPW+ +SRTV+++  + +LV+PAGWL W+G+FAL+TLYYGEY N G  
Sbjct: 458 --------TYLGRPWRSHSRTVFMKCNLGALVSPAGWLPWSGSFALSTLYYGEYGNTGAG 517

Query: 516 SSTANRVKWKGYRVITSVTEASKFTVQNFIAGKSWLPPTGVPFTASL 522
           +S + RVKW GY VI +VTEA KFTV+NF+ G  W+  TGVP    L
Sbjct: 518 ASVSGRVKWPGYHVIKTVTEAEKFTVENFLDGNYWITATGVPVNDGL 554

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O048879.8e-17761.58Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1[more]
O221496.4e-16055.92Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3... [more]
Q9FHN56.9e-15453.15Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3... [more]
Q9FHN48.4e-15253.18Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3... [more]
Q9STY31.5e-14551.56Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
XP_022996028.11.2e-25184.70pectinesterase-like [Cucurbita maxima][more]
XP_038875884.11.7e-25084.00pectinesterase 2-like [Benincasa hispida][more]
XP_022957920.12.4e-24983.75pectinesterase-like [Cucurbita moschata][more]
XP_023534348.17.1e-24983.37pectinesterase-like [Cucurbita pepo subsp. pepo][more]
KAG6605649.16.0e-24884.68Pectinesterase 2, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
A0A6J1K9P35.7e-25284.70Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111491356 PE=3 SV=1[more]
A0A6J1H1Z91.2e-24983.75Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459307 PE=3 SV=1[more]
A0A1S3ASJ44.8e-24381.52Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103482536 PE=3 SV=1[more]
A0A0A0KPG94.1e-24281.14Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G167190 PE=3 SV=1[more]
A0A5A7ULB71.5e-22084.77Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00120... [more]
Match NameE-valueIdentityDescription
AT2G45220.14.5e-16155.92Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G51490.14.9e-15553.15Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT5G51500.16.0e-15353.18Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT3G47400.11.1e-14651.56Plant invertase/pectin methylesterase inhibitor superfamily [more]
AT1G23200.14.7e-12647.63Plant invertase/pectin methylesterase inhibitor superfamily [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006501Pectinesterase inhibitor domainSMARTSM00856PMEI_2coord: 27..174
e-value: 7.0E-32
score: 121.9
IPR006501Pectinesterase inhibitor domainTIGRFAMTIGR01614TIGR01614coord: 12..176
e-value: 2.2E-24
score: 83.7
IPR006501Pectinesterase inhibitor domainPFAMPF04043PMEIcoord: 31..174
e-value: 1.0E-29
score: 103.9
IPR000070Pectinesterase, catalyticPFAMPF01095Pectinesterasecoord: 211..506
e-value: 1.7E-144
score: 480.1
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilyGENE3D1.20.140.40Invertase/pectin methylesterase inhibitor family proteincoord: 28..177
e-value: 1.7E-30
score: 108.0
IPR035513Invertase/pectin methylesterase inhibitor domain superfamilySUPERFAMILY101148Plant invertase/pectin methylesterase inhibitorcoord: 30..177
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 207..521
e-value: 1.7E-125
score: 420.6
NoneNo IPR availablePANTHERPTHR31707PECTINESTERASEcoord: 9..519
NoneNo IPR availablePANTHERPTHR31707:SF181PECTINESTERASEcoord: 9..519
NoneNo IPR availableCDDcd15798PMEI-like_3coord: 34..176
e-value: 8.01868E-40
score: 139.881
IPR033131Pectinesterase, Asp active sitePROSITEPS00503PECTINESTERASE_2coord: 354..363
IPR018040Pectinesterase, Tyr active sitePROSITEPS00800PECTINESTERASE_1coord: 235..254
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 207..519

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0005553.1Tan0005553.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042545 cell wall modification
biological_process GO:0043086 negative regulation of catalytic activity
biological_process GO:0045490 pectin catabolic process
cellular_component GO:0005576 extracellular region
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0045330 aspartyl esterase activity
molecular_function GO:0030599 pectinesterase activity
molecular_function GO:0046910 pectinesterase inhibitor activity
molecular_function GO:0004857 enzyme inhibitor activity