Tan0005516 (gene) Snake gourd v1

Overview


Name	Tan0005516
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	rRNA N-glycosidase
Location	LG06: 31192813 .. 31193736 (+)
RNA-Seq Expression	Tan0005516
Synteny	Tan0005516

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTGCCTGAGGTCTACTCAACCCTTCATACAATCCTTTCCCTACAATGATGGCGCTCATGCTCCCAACGCTAACGCTGGCGCCCAAGCAGCCCATGCAGTCAACTTCTCTCTGTTAGGTGCCACAGGTGAAACTTACAAACACTTCATAAAGAATTTACGTCAGGTTCTCACAGCCAACTCGCACAAGGTGTACAGCATACCTGTGCTACCAGCCACGGCCTCGGGGTCGGTGCGCTTCGTATTAGTTAACATTTATGGTGTGGCGTATCAAGCTAGAAACATTGCCTATTTTCTGAATGACGCTTCAAAAGAAGCAAAAAGCGTGCTATTTAAAGGCATCAAGCATGAAATACTTCCCTATAAAGGCAATTACGACGGCCCCGAGTCGGCTGGAGGGAAGATTTCAAGAGAGAAAATCGATCTTGGATTTTCTGAATTAAGTAGTTCTATTGGCAACATGTTTCACCATCAACCAGGTACTTCTATCCCTAAGGCATTTATTGTGATAATTCAGATGGTTTCAGAAGCGGCGAGATTTAAATATAATGAGCAAAGGGTTTCTGAGAGTGTTGACAATAAATTTAGGCCAGATACAGGATTCTTAAGTTTAGAAAACAAATGGTCTAATCTATCAGAACAAATACAAATCGCACAGAAACGAGGAGGGAAGTTTGCTCGTCCCGTTGAGGTTCGAAGTGTTAAGAACAACCCAATTCAAGTAACCGATGTTAAATCAAAAGTTGTGAAAGGTATCGCACTGTTACTTCACAGCAAGTCAGGGGCAAATGGGCATCAAATGAAACTGATGATGATGCCAGAGCTTAATGAATGGGAATATGTTGCGACTGGAGATGAAATGAAACTGATTGATGTTGAGGATCCACGTTGTAAAAGTGGAGAGAAACCTCACAATATATAA

mRNA sequence

ATGGAGTGCCTGAGGTCTACTCAACCCTTCATACAATCCTTTCCCTACAATGATGGCGCTCATGCTCCCAACGCTAACGCTGGCGCCCAAGCAGCCCATGCAGTCAACTTCTCTCTGTTAGGTGCCACAGGTGAAACTTACAAACACTTCATAAAGAATTTACGTCAGGTTCTCACAGCCAACTCGCACAAGGTGTACAGCATACCTGTGCTACCAGCCACGGCCTCGGGGTCGGTGCGCTTCGTATTAGTTAACATTTATGGTGTGGCGTATCAAGCTAGAAACATTGCCTATTTTCTGAATGACGCTTCAAAAGAAGCAAAAAGCGTGCTATTTAAAGGCATCAAGCATGAAATACTTCCCTATAAAGGCAATTACGACGGCCCCGAGTCGGCTGGAGGGAAGATTTCAAGAGAGAAAATCGATCTTGGATTTTCTGAATTAAGTAGTTCTATTGGCAACATGTTTCACCATCAACCAGGTACTTCTATCCCTAAGGCATTTATTGTGATAATTCAGATGGTTTCAGAAGCGGCGAGATTTAAATATAATGAGCAAAGGGTTTCTGAGAGTGTTGACAATAAATTTAGGCCAGATACAGGATTCTTAAGTTTAGAAAACAAATGGTCTAATCTATCAGAACAAATACAAATCGCACAGAAACGAGGAGGGAAGTTTGCTCGTCCCGTTGAGGTTCGAAGTGTTAAGAACAACCCAATTCAAGTAACCGATGTTAAATCAAAAGTTGTGAAAGGTATCGCACTGTTACTTCACAGCAAGTCAGGGGCAAATGGGCATCAAATGAAACTGATGATGATGCCAGAGCTTAATGAATGGGAATATGTTGCGACTGGAGATGAAATGAAACTGATTGATGTTGAGGATCCACGTTGTAAAAGTGGAGAGAAACCTCACAATATATAA

Coding sequence (CDS)

ATGGAGTGCCTGAGGTCTACTCAACCCTTCATACAATCCTTTCCCTACAATGATGGCGCTCATGCTCCCAACGCTAACGCTGGCGCCCAAGCAGCCCATGCAGTCAACTTCTCTCTGTTAGGTGCCACAGGTGAAACTTACAAACACTTCATAAAGAATTTACGTCAGGTTCTCACAGCCAACTCGCACAAGGTGTACAGCATACCTGTGCTACCAGCCACGGCCTCGGGGTCGGTGCGCTTCGTATTAGTTAACATTTATGGTGTGGCGTATCAAGCTAGAAACATTGCCTATTTTCTGAATGACGCTTCAAAAGAAGCAAAAAGCGTGCTATTTAAAGGCATCAAGCATGAAATACTTCCCTATAAAGGCAATTACGACGGCCCCGAGTCGGCTGGAGGGAAGATTTCAAGAGAGAAAATCGATCTTGGATTTTCTGAATTAAGTAGTTCTATTGGCAACATGTTTCACCATCAACCAGGTACTTCTATCCCTAAGGCATTTATTGTGATAATTCAGATGGTTTCAGAAGCGGCGAGATTTAAATATAATGAGCAAAGGGTTTCTGAGAGTGTTGACAATAAATTTAGGCCAGATACAGGATTCTTAAGTTTAGAAAACAAATGGTCTAATCTATCAGAACAAATACAAATCGCACAGAAACGAGGAGGGAAGTTTGCTCGTCCCGTTGAGGTTCGAAGTGTTAAGAACAACCCAATTCAAGTAACCGATGTTAAATCAAAAGTTGTGAAAGGTATCGCACTGTTACTTCACAGCAAGTCAGGGGCAAATGGGCATCAAATGAAACTGATGATGATGCCAGAGCTTAATGAATGGGAATATGTTGCGACTGGAGATGAAATGAAACTGATTGATGTTGAGGATCCACGTTGTAAAAGTGGAGAGAAACCTCACAATATATAA

Protein sequence

MECLRSTQPFIQSFPYNDGAHAPNANAGAQAAHAVNFSLLGATGETYKHFIKNLRQVLTANSHKVYSIPVLPATASGSVRFVLVNIYGVAYQARNIAYFLNDASKEAKSVLFKGIKHEILPYKGNYDGPESAGGKISREKIDLGFSELSSSIGNMFHHQPGTSIPKAFIVIIQMVSEAARFKYNEQRVSESVDNKFRPDTGFLSLENKWSNLSEQIQIAQKRGGKFARPVEVRSVKNNPIQVTDVKSKVVKGIALLLHSKSGANGHQMKLMMMPELNEWEYVATGDEMKLIDVEDPRCKSGEKPHNI

Homology

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BLAST of Tan0005516 vs. ExPASy Swiss-Prot
Match: P98184 (Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1)

HSP 1 Score: 290.8 bits (743), Expect = 1.8e-77
Identity = 154/241 (63.90%), Postives = 179/241 (74.27%), Query Frame = 0


Query: 35  VNFSLLGATGETYKHFIKNLRQVLTANSHKVYSIPVLPATASGSVRFVL----------- 94
           +NFSL+GATG TYK FI+NLR  LT  + +VY IPVL   A+G  RF L           
Sbjct: 24  INFSLIGATGATYKTFIRNLRTKLTVGTPRVYDIPVLRNAAAGLARFQLVTLTNYNGESV 83

Query: 95  ------VNIYGVAYQARNIAYFLNDASKEAKSVLFKGIKHEILPYKGNYDGPESAGGKIS 154
                 VN+Y VAY+A N AYFL DAS EA +VLF GI H  LPY GNYDG E+A G+IS
Sbjct: 84  TVALDVVNVYVVAYRAGNTAYFLADASTEANNVLFAGINHVRLPYGGNYDGLETAAGRIS 143

Query: 155 REKIDLGFSELSSSIGNMFHHQPGTSIPKAFIVIIQMVSEAARFKYNEQRVSESVDNKFR 214
           RE I+LGFSE+SS+IGNMF H PGTS+P+AFIVIIQ VSEAARFKY EQRVSE+V  KF+
Sbjct: 144 RENIELGFSEISSAIGNMFRHNPGTSVPRAFIVIIQTVSEAARFKYIEQRVSENVGTKFK 203

Query: 215 PDTGFLSLENKWSNLSEQIQIAQKRGGKFARPVEVRSVKNNPIQVTDVKSKVVKGIALLL 259
           PD  FLSL+N W +LSEQIQIAQ RGG+FARPVE+R+V N P  VT+V S VVKGIALLL
Sbjct: 204 PDPAFLSLQNAWGSLSEQIQIAQTRGGEFARPVELRTVSNTPTFVTNVNSPVVKGIALLL 263

[Close]

BLAST of Tan0005516 vs. ExPASy Swiss-Prot
Match: P29339 (Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 3.6e-46
Identity = 110/245 (44.90%), Postives = 153/245 (62.45%), Query Frame = 0


Query: 35  VNFSLLGATGETYKHFIKNLRQVLTANSHKVYSIPVLPATASGSVRFVLV---------- 94
           VNF L  AT +TY  FI++ R  L   SHKVY IP+L +T S S RF+L+          
Sbjct: 25  VNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFILLDLTSYAYETI 84

Query: 95  -------NIYGVAYQARNIAYFLNDASKEAKSVLFKGIKHEILPYKGNYDGPESAGGKIS 154
                  N+Y VAY+ R+++YF  ++  EA ++LFKG +   LPY GNY+  ++A  KI 
Sbjct: 85  SVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQTAAHKI- 144

Query: 155 REKIDLGFSELSSSIGNMFHHQPGTSIPKAFIVIIQMVSEAARFKYNEQRVSESVDNKFR 214
           RE IDLG   LSS+I  +F++    S P A +V+IQ  +EAARFKY E+ V++ V   F+
Sbjct: 145 RENIDLGLPALSSAITTLFYYN-AQSAPSALLVLIQTTAEAARFKYIERHVAKYVATNFK 204

Query: 215 PDTGFLSLENKWSNLSEQIQIAQKRGGKFARPVEVRSVKNNPIQVTDVKSKVVKG-IALL 262
           P+   +SLEN+WS LS+QI +AQ +GGKF  PV++        QVT+V S VVKG I LL
Sbjct: 205 PNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVKGNIKLL 264

[Close]

BLAST of Tan0005516 vs. ExPASy Swiss-Prot
Match: P24817 (Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN=MAP30 PE=1 SV=2)

HSP 1 Score: 186.8 bits (473), Expect = 3.6e-46
Identity = 110/245 (44.90%), Postives = 153/245 (62.45%), Query Frame = 0


Query: 35  VNFSLLGATGETYKHFIKNLRQVLTANSHKVYSIPVLPATASGSVRFVLV---------- 94
           VNF L  AT +TY  FI++ R  L   SHKVY IP+L +T S S RF+L+          
Sbjct: 25  VNFDLSTATAKTYTKFIEDFRATLPF-SHKVYDIPLLYSTISDSRRFILLNLTSYAYETI 84

Query: 95  -------NIYGVAYQARNIAYFLNDASKEAKSVLFKGIKHEILPYKGNYDGPESAGGKIS 154
                  N+Y VAY+ R+++YF  ++  EA ++LFKG +   LPY GNY+  ++A  KI 
Sbjct: 85  SVAIDVTNVYVVAYRTRDVSYFFKESPPEAYNILFKGTRKITLPYTGNYENLQTAAHKI- 144

Query: 155 REKIDLGFSELSSSIGNMFHHQPGTSIPKAFIVIIQMVSEAARFKYNEQRVSESVDNKFR 214
           RE IDLG   LSS+I  +F++    S P A +V+IQ  +EAARFKY E+ V++ V   F+
Sbjct: 145 RENIDLGLPALSSAITTLFYYN-AQSAPSALLVLIQTTAEAARFKYIERHVAKYVATNFK 204

Query: 215 PDTGFLSLENKWSNLSEQIQIAQKRGGKFARPVEVRSVKNNPIQVTDVKSKVVKG-IALL 262
           P+   +SLEN+WS LS+QI +AQ +GGKF  PV++        QVT+V S VVKG I LL
Sbjct: 205 PNLAIISLENQWSALSKQIFLAQNQGGKFRNPVDLIKPTGERFQVTNVDSDVVKGNIKLL 264

[Close]

BLAST of Tan0005516 vs. ExPASy Swiss-Prot
Match: P84531 (Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2)

HSP 1 Score: 171.0 bits (432), Expect = 2.1e-41
Identity = 100/242 (41.32%), Postives = 141/242 (58.26%), Query Frame = 0


Query: 35  VNFSLLGATGETYKHFIKNLRQVLTANSHKVYSIPVLPATASGSVRFVLV---------- 94
           V F L  AT  +YK FIKNLR+ L  +  KVY IPVL +T   S RF+L+          
Sbjct: 2   VRFDLSSATSSSYKTFIKNLREALPKDG-KVYDIPVLLSTVMDSRRFILIDLVNYDGQSI 61

Query: 95  -------NIYGVAYQARNIAYFLNDASKEAKSVLFKGIKHEILPYKGNYDGPESAGGKIS 154
                  N+Y VAY    ++YF      +A  +LFKG +   LPY GNY+  ++A  K+ 
Sbjct: 62  TAAIDVLNVYIVAYSTGTVSYFFQQVPAQAPKLLFKGTQQRTLPYTGNYENLQTAAKKL- 121

Query: 155 REKIDLGFSELSSSIGNMFHHQPGTSIPKAFIVIIQMVSEAARFKYNEQRVSESVDNKFR 214
           RE I+LG   L S+I  +FH+    +   A +V+IQ  SEAARF+Y E +++ +V  KF+
Sbjct: 122 RENIELGLPALDSAITTLFHYN-AEAAASALLVLIQTTSEAARFRYIELQIANNVGTKFK 181

Query: 215 PDTGFLSLENKWSNLSEQIQIAQKRGGKFARPVEVRSVKNNPIQVTDVKSKVV-KGIALL 259
           P    +SLEN WS LS+QIQIA+ + G+F  PV +   + N +Q+T+V S VV + I LL
Sbjct: 182 PSQTIISLENNWSALSKQIQIAKNKNGQFETPVILIDPQGNRVQITNVTSNVVTQNIQLL 240

[Close]

BLAST of Tan0005516 vs. ExPASy Swiss-Prot
Match: Q00465 (Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 160.6 bits (405), Expect = 2.8e-38
Identity = 100/245 (40.82%), Postives = 144/245 (58.78%), Query Frame = 0


Query: 35  VNFSLLGATGETYKHFIKNLRQVLTANSHKVYSIPVLPATASGSVRFVLV---------- 94
           V FSL G++  +Y  FI +LR+ L +N   VY+I +L ++ASG+ R+ L+          
Sbjct: 22  VRFSLSGSSSTSYSKFIGDLRKALPSNG-TVYNITLLLSSASGASRYTLMTLSNYDGKAI 81

Query: 95  -------NIYGVAYQARNIAYFLNDA-SKEAKSVLFKGIKHEILPYKGNYDGPESAGGKI 154
                  N+Y + Y   + +YF N++ +K A   +FKG     LPY GNY+  ++A GKI
Sbjct: 82  TVAVDVTNVYIMGYLVNSTSYFFNESDAKLASQYVFKGSTIVTLPYSGNYEKLQTAAGKI 141

Query: 155 SREKIDLGFSELSSSIGNMFHHQPGTSIPKAFIVIIQMVSEAARFKYNEQRVSESVDNKF 214
            REKI LGF  L S+I  +FH+   T+   AF+VIIQ  +EA+RFKY E ++ E +    
Sbjct: 142 -REKIPLGFPALDSAITTLFHYD-STAAAAAFLVIIQTTAEASRFKYIEGQIIERISKNQ 201

Query: 215 RPDTGFLSLENKWSNLSEQIQIAQKRGGKFARPVEVRSVKNNPIQVTDVKSKVV-KGIAL 261
            P    +SLEN+WS LS+QIQ+AQ   G F  PV +   K   +++T+V SKVV K I L
Sbjct: 202 VPSLATISLENEWSALSKQIQLAQTNNGTFKTPVVITDDKGQRVEITNVTSKVVTKNIQL 261

[Close]

BLAST of Tan0005516 vs. NCBI nr
Match: XP_022158191.1 (ribosome-inactivating protein bryodin II-like [Momordica charantia])

HSP 1 Score: 358.2 bits (918), Expect = 7.0e-95
Identity = 192/300 (64.00%), Postives = 230/300 (76.67%), Query Frame = 0


Query: 1   MECLRSTQPFIQSFPYND-GAHAPNANAGAQAAHA-VNFSLLGATGETYKHFIKNLRQVL 60
           MEC+RS++PFIQ  P+ND G H  +AN    A ++ V+FSLLGATG+TY+ FI+NLR  L
Sbjct: 1   MECVRSSEPFIQPLPFNDIGIHGVHANGAVNAPNSNVSFSLLGATGKTYQQFIQNLRNAL 60

Query: 61  TANSHKVYSIPVLPATASGSVRFVL-----------------VNIYGVAYQARNIAYFLN 120
           T NS  VY+IPVL ATA  S RF+L                 VN+Y VAYQA N AYFL 
Sbjct: 61  TINSKIVYNIPVLAATALSSARFILVHLTNYKNETITVAIDVVNVYIVAYQAGNKAYFLK 120

Query: 121 DASKEAKSVLFKGIKHEILPYKGNYDGPESAGGKISREKIDLGFSELSSSIGNMFHHQPG 180
           DASKEA+ VLFKGIK EILPYKGNYDG E+A GKISREKIDLGFSEL S+IGNM+H+  G
Sbjct: 121 DASKEARDVLFKGIKQEILPYKGNYDGLETAAGKISREKIDLGFSELGSAIGNMYHYNAG 180

Query: 181 TSIPKAFIVIIQMVSEAARFKYNEQRVSESVDNKFRPDTGFLSLENKWSNLSEQIQIAQK 240
           TS+P+AFIV+IQ +SEAARFKY E +VS++V+ KF+PD GFLSLEN+WS+LSEQ+QIAQ 
Sbjct: 181 TSVPRAFIVMIQTISEAARFKYIETKVSQNVETKFKPDPGFLSLENRWSDLSEQVQIAQN 240

Query: 241 RGGKFARPVEVRSVKNNPIQVTDVKSKVVKGIALLLHSKSGANGHQMKLMMMPELN-EWE 281
           R G+FARP+EVRSV N PI VT+VKSKVV+G+ALLL+SK   NG  M+L  M E N EWE
Sbjct: 241 RKGEFARPIEVRSVTNKPILVTNVKSKVVEGMALLLYSKPRGNGDLMEL--MEEFNKEWE 298

[Close]

BLAST of Tan0005516 vs. NCBI nr
Match: KAG6607631.1 (hypothetical protein SDJN03_00973, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 332.4 bits (851), Expect = 4.1e-87
Identity = 181/289 (62.63%), Postives = 208/289 (71.97%), Query Frame = 0


Query: 1   MECLRSTQPFIQS-FPYNDGAHA------PNANAGAQAAHAVNFSLLGATGETYKHFIKN 60
           MEC+ STQ  IQ+ FP N GAHA      P+ANA       ++FSLLGAT +TYK FI +
Sbjct: 1   MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANAN------LSFSLLGATAQTYKQFIND 60

Query: 61  LRQVLTANSHKVYSIPVLPATASGSVRFVLVN-----------------IYGVAYQARNI 120
           LR+ LT NSHKVY IPVLPATA G  RF+LVN                 +Y VAYQA N 
Sbjct: 61  LRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNK 120

Query: 121 AYFLNDASKEAKSVLFKGIKHEILPYKGNYDGPESAGGKISREKIDLGFSELSSSIGNMF 180
           AYFL D SKEA  VLFKG KHE L YKGNYD  E   GKISRE I+LGFSELSSSIGNMF
Sbjct: 121 AYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMF 180

Query: 181 HHQPGTSIPKAFIVIIQMVSEAARFKYNEQRVSESVDNKFRPDTGFLSLENKWSNLSEQI 240
           H+  G S+P++FIV+IQM+SEA+RFKY E R  E V++KF+PD G+LSLEN+WSNLSE +
Sbjct: 181 HYNAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEHV 240

Query: 241 QIAQKRGGKFARPVEVRSVKNNPIQVTDVKSKVVKGIALLLHSKSGANG 266
           Q AQK GGKF RPV+VRSV N PI+VTDV S VVKGIALLL +K G NG
Sbjct: 241 QKAQKHGGKFERPVQVRSVSNKPIKVTDVNSPVVKGIALLLCNKPGVNG 283

[Close]

BLAST of Tan0005516 vs. NCBI nr
Match: XP_022926255.1 (ribosome-inactivating protein bryodin II-like [Cucurbita moschata])

HSP 1 Score: 332.4 bits (851), Expect = 4.1e-87
Identity = 183/304 (60.20%), Postives = 213/304 (70.07%), Query Frame = 0


Query: 1   MECLRSTQPFIQS-FPYNDGAHA------PNANAGAQAAHAVNFSLLGATGETYKHFIKN 60
           MEC+ STQ  IQ+ FP N GAHA      P+ANA       ++FSLLGAT +TYK FI +
Sbjct: 1   MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANAN------LSFSLLGATAQTYKQFIND 60

Query: 61  LRQVLTANSHKVYSIPVLPATASGSVRFVLVN-----------------IYGVAYQARNI 120
           LR+ LT NSHKVY IPVLPATA G  RF+LVN                 +Y VAYQA N 
Sbjct: 61  LRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNK 120

Query: 121 AYFLNDASKEAKSVLFKGIKHEILPYKGNYDGPESAGGKISREKIDLGFSELSSSIGNMF 180
           AYFL D SKEA  VLFKG KHE L YKGNYD  E   GKISRE I+LGFSELSSSIGNMF
Sbjct: 121 AYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMF 180

Query: 181 HHQPGTSIPKAFIVIIQMVSEAARFKYNEQRVSESVDNKFRPDTGFLSLENKWSNLSEQI 240
           H++ G S+P++FIV+IQM+SEA+RFKY E R  E V++KF+PD G+LSLEN+WSNLSEQ+
Sbjct: 181 HYKAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQV 240

Query: 241 QIAQKRGGKFARPVEVRSVKNNPIQVTDVKSKVVKGIALLLHSKSGANGHQMKLMMMPEL 281
           Q AQK GGKF  PV+VRSV N P+ V+DV S VVKGIALLL +K G NG      +M E 
Sbjct: 241 QKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLSNKPGVNGVS---KLMEEF 295

[Close]

BLAST of Tan0005516 vs. NCBI nr
Match: KAG7037232.1 (hypothetical protein SDJN02_00855, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 331.6 bits (849), Expect = 7.0e-87
Identity = 183/304 (60.20%), Postives = 213/304 (70.07%), Query Frame = 0


Query: 1   MECLRSTQPFIQS-FPYNDGAHA------PNANAGAQAAHAVNFSLLGATGETYKHFIKN 60
           MEC+ STQ  IQ+ FP N GAHA      P+ANA       ++FSLLGAT +TYK FI +
Sbjct: 1   MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANAN------LSFSLLGATAQTYKQFIND 60

Query: 61  LRQVLTANSHKVYSIPVLPATASGSVRFVLVN-----------------IYGVAYQARNI 120
           LR+ LT NSHKVY IPVLPATA G  RF+LVN                 +Y VAYQA N 
Sbjct: 61  LRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNK 120

Query: 121 AYFLNDASKEAKSVLFKGIKHEILPYKGNYDGPESAGGKISREKIDLGFSELSSSIGNMF 180
           AYFL D SKEA  VLFKG KHE L YKGNYD  E   GKISRE I+LGFSELSSSIGNMF
Sbjct: 121 AYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMF 180

Query: 181 HHQPGTSIPKAFIVIIQMVSEAARFKYNEQRVSESVDNKFRPDTGFLSLENKWSNLSEQI 240
           H+  G S+P++FIV+IQM+SEA+RFKY E R  E V++KF+PD G+LSLEN+WSNLSEQ+
Sbjct: 181 HYNAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQV 240

Query: 241 QIAQKRGGKFARPVEVRSVKNNPIQVTDVKSKVVKGIALLLHSKSGANGHQMKLMMMPEL 281
           Q AQK GGKF  PV+VRSV N P+ V+DV S VVKGIALLL +K G NG      +M E 
Sbjct: 241 QKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLCNKPGVNGVS---KLMEEF 295

[Close]

BLAST of Tan0005516 vs. NCBI nr
Match: XP_022981562.1 (ribosome-inactivating protein bryodin II-like [Cucurbita maxima])

HSP 1 Score: 330.9 bits (847), Expect = 1.2e-86
Identity = 182/302 (60.26%), Postives = 213/302 (70.53%), Query Frame = 0


Query: 1   MECLRSTQPFIQS-FPYNDGAH----APNANAGAQAAHAVNFSLLGATGETYKHFIKNLR 60
           MEC+ ST+  IQ+ FP N GAH    AP  +A A     ++FSLLGAT +TYK FI +LR
Sbjct: 1   MECIGSTKDLIQAPFPLNCGAHVAIRAPRPSANAN----LSFSLLGATAQTYKQFISDLR 60

Query: 61  QVLTANSHKVYSIPVLPATASGSVRFVLVN-----------------IYGVAYQARNIAY 120
           + LT NSHKVY IPVLPATA G  RF+LVN                 +Y VAYQA N AY
Sbjct: 61  KALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAY 120

Query: 121 FLNDASKEAKSVLFKGIKHEILPYKGNYDGPESAGGKISREKIDLGFSELSSSIGNMFHH 180
           FL D SKEA  VLFKG KHE L YKGNYD  E   GKISRE IDLGFSELSSSIGNMFH+
Sbjct: 121 FLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIDLGFSELSSSIGNMFHY 180

Query: 181 QPGTSIPKAFIVIIQMVSEAARFKYNEQRVSESVDNKFRPDTGFLSLENKWSNLSEQIQI 240
             G S+PK+FIV+IQM+SEA+RFKY E R  E+V++KF+PD G+LSLEN+WSNLSEQ+Q 
Sbjct: 181 NAGYSVPKSFIVMIQMLSEASRFKYIEARNCENVESKFKPDPGYLSLENRWSNLSEQVQK 240

Query: 241 AQKRGGKFARPVEVRSVKNNPIQVTDVKSKVVKGIALLLHSKSGANGHQMKLMMMPELNE 281
           AQK GGKF  PV+VRSV N P+ V+DV S VVKG+ALLL +K G NG      +M E  +
Sbjct: 241 AQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGMALLLCNKPGVNGVS---KLMEEFIK 295

[Close]

BLAST of Tan0005516 vs. ExPASy TrEMBL
Match: A0A6J1E093 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1)

HSP 1 Score: 358.2 bits (918), Expect = 3.4e-95
Identity = 192/300 (64.00%), Postives = 230/300 (76.67%), Query Frame = 0


Query: 1   MECLRSTQPFIQSFPYND-GAHAPNANAGAQAAHA-VNFSLLGATGETYKHFIKNLRQVL 60
           MEC+RS++PFIQ  P+ND G H  +AN    A ++ V+FSLLGATG+TY+ FI+NLR  L
Sbjct: 1   MECVRSSEPFIQPLPFNDIGIHGVHANGAVNAPNSNVSFSLLGATGKTYQQFIQNLRNAL 60

Query: 61  TANSHKVYSIPVLPATASGSVRFVL-----------------VNIYGVAYQARNIAYFLN 120
           T NS  VY+IPVL ATA  S RF+L                 VN+Y VAYQA N AYFL 
Sbjct: 61  TINSKIVYNIPVLAATALSSARFILVHLTNYKNETITVAIDVVNVYIVAYQAGNKAYFLK 120

Query: 121 DASKEAKSVLFKGIKHEILPYKGNYDGPESAGGKISREKIDLGFSELSSSIGNMFHHQPG 180
           DASKEA+ VLFKGIK EILPYKGNYDG E+A GKISREKIDLGFSEL S+IGNM+H+  G
Sbjct: 121 DASKEARDVLFKGIKQEILPYKGNYDGLETAAGKISREKIDLGFSELGSAIGNMYHYNAG 180

Query: 181 TSIPKAFIVIIQMVSEAARFKYNEQRVSESVDNKFRPDTGFLSLENKWSNLSEQIQIAQK 240
           TS+P+AFIV+IQ +SEAARFKY E +VS++V+ KF+PD GFLSLEN+WS+LSEQ+QIAQ 
Sbjct: 181 TSVPRAFIVMIQTISEAARFKYIETKVSQNVETKFKPDPGFLSLENRWSDLSEQVQIAQN 240

Query: 241 RGGKFARPVEVRSVKNNPIQVTDVKSKVVKGIALLLHSKSGANGHQMKLMMMPELN-EWE 281
           R G+FARP+EVRSV N PI VT+VKSKVV+G+ALLL+SK   NG  M+L  M E N EWE
Sbjct: 241 RKGEFARPIEVRSVTNKPILVTNVKSKVVEGMALLLYSKPRGNGDLMEL--MEEFNKEWE 298

[Close]

BLAST of Tan0005516 vs. ExPASy TrEMBL
Match: A0A6J1EKL0 (rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111433434 PE=3 SV=1)

HSP 1 Score: 332.4 bits (851), Expect = 2.0e-87
Identity = 183/304 (60.20%), Postives = 213/304 (70.07%), Query Frame = 0


Query: 1   MECLRSTQPFIQS-FPYNDGAHA------PNANAGAQAAHAVNFSLLGATGETYKHFIKN 60
           MEC+ STQ  IQ+ FP N GAHA      P+ANA       ++FSLLGAT +TYK FI +
Sbjct: 1   MECIGSTQDLIQAPFPLNCGAHAAIRAPRPSANAN------LSFSLLGATAQTYKQFIND 60

Query: 61  LRQVLTANSHKVYSIPVLPATASGSVRFVLVN-----------------IYGVAYQARNI 120
           LR+ LT NSHKVY IPVLPATA G  RF+LVN                 +Y VAYQA N 
Sbjct: 61  LRKALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNK 120

Query: 121 AYFLNDASKEAKSVLFKGIKHEILPYKGNYDGPESAGGKISREKIDLGFSELSSSIGNMF 180
           AYFL D SKEA  VLFKG KHE L YKGNYD  E   GKISRE I+LGFSELSSSIGNMF
Sbjct: 121 AYFLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIELGFSELSSSIGNMF 180

Query: 181 HHQPGTSIPKAFIVIIQMVSEAARFKYNEQRVSESVDNKFRPDTGFLSLENKWSNLSEQI 240
           H++ G S+P++FIV+IQM+SEA+RFKY E R  E V++KF+PD G+LSLEN+WSNLSEQ+
Sbjct: 181 HYKAGYSVPRSFIVMIQMLSEASRFKYIETRNCEKVESKFKPDPGYLSLENRWSNLSEQV 240

Query: 241 QIAQKRGGKFARPVEVRSVKNNPIQVTDVKSKVVKGIALLLHSKSGANGHQMKLMMMPEL 281
           Q AQK GGKF  PV+VRSV N P+ V+DV S VVKGIALLL +K G NG      +M E 
Sbjct: 241 QKAQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGIALLLSNKPGVNGVS---KLMEEF 295

[Close]

BLAST of Tan0005516 vs. ExPASy TrEMBL
Match: A0A6J1IUB2 (rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 5.8e-87
Identity = 182/302 (60.26%), Postives = 213/302 (70.53%), Query Frame = 0


Query: 1   MECLRSTQPFIQS-FPYNDGAH----APNANAGAQAAHAVNFSLLGATGETYKHFIKNLR 60
           MEC+ ST+  IQ+ FP N GAH    AP  +A A     ++FSLLGAT +TYK FI +LR
Sbjct: 1   MECIGSTKDLIQAPFPLNCGAHVAIRAPRPSANAN----LSFSLLGATAQTYKQFISDLR 60

Query: 61  QVLTANSHKVYSIPVLPATASGSVRFVLVN-----------------IYGVAYQARNIAY 120
           + LT NSHKVY IPVLPATA G  RF+LVN                 +Y VAYQA N AY
Sbjct: 61  KALTVNSHKVYDIPVLPATAKGLARFILVNLTNYKGESITVAIDTVDVYIVAYQAGNKAY 120

Query: 121 FLNDASKEAKSVLFKGIKHEILPYKGNYDGPESAGGKISREKIDLGFSELSSSIGNMFHH 180
           FL D SKEA  VLFKG KHE L YKGNYD  E   GKISRE IDLGFSELSSSIGNMFH+
Sbjct: 121 FLKDVSKEAHDVLFKGSKHETLSYKGNYDDLERHAGKISRENIDLGFSELSSSIGNMFHY 180

Query: 181 QPGTSIPKAFIVIIQMVSEAARFKYNEQRVSESVDNKFRPDTGFLSLENKWSNLSEQIQI 240
             G S+PK+FIV+IQM+SEA+RFKY E R  E+V++KF+PD G+LSLEN+WSNLSEQ+Q 
Sbjct: 181 NAGYSVPKSFIVMIQMLSEASRFKYIEARNCENVESKFKPDPGYLSLENRWSNLSEQVQK 240

Query: 241 AQKRGGKFARPVEVRSVKNNPIQVTDVKSKVVKGIALLLHSKSGANGHQMKLMMMPELNE 281
           AQK GGKF  PV+VRSV N P+ V+DV S VVKG+ALLL +K G NG      +M E  +
Sbjct: 241 AQKHGGKFEHPVQVRSVSNKPVLVSDVNSPVVKGMALLLCNKPGVNGVS---KLMEEFIK 295

[Close]

BLAST of Tan0005516 vs. ExPASy TrEMBL
Match: A0A6J1DWK4 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1)

HSP 1 Score: 310.5 bits (794), Expect = 8.1e-81
Identity = 170/251 (67.73%), Postives = 192/251 (76.49%), Query Frame = 0


Query: 28  GAQAAHA-VNFSLLGATGETYKHFIKNLRQVLTANSHKVYSIPVLPATASGSVRFVL--- 87
           GA AA A V+FS+LGAT ETY+ FI+NLR  LT  S  VY IPVL +TA GS RF+L   
Sbjct: 15  GALAAEADVSFSMLGATSETYRQFIRNLRSTLTIRSPVVYGIPVLQSTAVGSARFILVHL 74

Query: 88  --------------VNIYGVAYQARNIAYFLNDASKEAKSVLFKGIKHEILPYKGNYDGP 147
                         VNIY VAY+A N AYFLNDAS EA +VLFKGIKH  LPYKGNYDG 
Sbjct: 75  TNYNTESITVAIDVVNIYVVAYRAGNNAYFLNDASAEANNVLFKGIKHVRLPYKGNYDGL 134

Query: 148 ESAGGKISREKIDLGFSELSSSIGNMFHHQPGTSIPKAFIVIIQMVSEAARFKYNEQRVS 207
           E+A GKISREKIDLGFSE+SSSIGNMFHH  GTSI +AFIVIIQ V EAARFKY EQRVS
Sbjct: 135 ETAAGKISREKIDLGFSEISSSIGNMFHHNVGTSIARAFIVIIQSVLEAARFKYIEQRVS 194

Query: 208 ESVDNKFRPDTGFLSLENKWSNLSEQIQIAQKRGGKFARPVEVRSVKNNPIQVTDVKSKV 261
           E+V  KF+PD  FLSLEN+WS+LSEQIQIAQKR G+FAR +E+R+V N PI VT+V S +
Sbjct: 195 ENVKTKFKPDPAFLSLENRWSDLSEQIQIAQKREGEFARAIELRTVSNKPIIVTNVSSPI 254

[Close]

BLAST of Tan0005516 vs. ExPASy TrEMBL
Match: A0A6J1DVE7 (rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 6.8e-80
Identity = 169/251 (67.33%), Postives = 192/251 (76.49%), Query Frame = 0


Query: 28  GAQAAHA-VNFSLLGATGETYKHFIKNLRQVLTANSHKVYSIPVLPATASGSVRFVL--- 87
           GA+AA A V+FS+LG+T ETYK FIKN+R  LT  S  VY IPVL ATA+GS RF+L   
Sbjct: 15  GARAAEADVSFSMLGSTSETYKQFIKNVRSALTVGSQIVYDIPVLKATATGSARFLLVHL 74

Query: 88  --------------VNIYGVAYQARNIAYFLNDASKEAKSVLFKGIKHEILPYKGNYDGP 147
                         VN+Y VAY+A N AYFLNDAS EA  VLF+GI H  L YKGNYDG 
Sbjct: 75  TNYNSESIIVAIDVVNVYVVAYRAGNNAYFLNDASAEADRVLFQGINHVKLSYKGNYDGL 134

Query: 148 ESAGGKISREKIDLGFSELSSSIGNMFHHQPGTSIPKAFIVIIQMVSEAARFKYNEQRVS 207
           E+A  KISREKIDLGFSE+SSSIGNMFHH  GTS+P+AFIVIIQ VSEAARFKY EQRVS
Sbjct: 135 ETA-AKISREKIDLGFSEISSSIGNMFHHNAGTSVPRAFIVIIQSVSEAARFKYIEQRVS 194

Query: 208 ESVDNKFRPDTGFLSLENKWSNLSEQIQIAQKRGGKFARPVEVRSVKNNPIQVTDVKSKV 261
           E+V   F+PD  FLSL+N+WS+LSEQIQIAQKRGG+FAR VE+R+V N PI VTDV S V
Sbjct: 195 ENVKTTFKPDPAFLSLQNRWSDLSEQIQIAQKRGGEFARAVELRTVSNKPILVTDVSSPV 254

The following BLAST results are available for this feature:

BLAST of Tan0005516 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P98184	1.8e-77	63.90	Ribosome-inactivating protein bryodin II OS=Bryonia dioica OX=3652 PE=1 SV=1	[more]
P29339	3.6e-46	44.90	Ribosome-inactivating protein momordin II OS=Momordica balsamina OX=3672 PE=3 SV...	[more]
P24817	3.6e-46	44.90	Ribosome-inactivating protein beta-momorcharin OS=Momordica charantia OX=3673 GN...	[more]
P84531	2.1e-41	41.32	Ribosome-inactivating protein cucurmosin OS=Cucurbita moschata OX=3662 PE=1 SV=2	[more]
Q00465	2.8e-38	40.82	Ribosome-inactivating protein luffin-alpha OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]

BLAST of Tan0005516 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022158191.1	7.0e-95	64.00	ribosome-inactivating protein bryodin II-like [Momordica charantia]	[more]
KAG6607631.1	4.1e-87	62.63	hypothetical protein SDJN03_00973, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022926255.1	4.1e-87	60.20	ribosome-inactivating protein bryodin II-like [Cucurbita moschata]	[more]
KAG7037232.1	7.0e-87	60.20	hypothetical protein SDJN02_00855, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022981562.1	1.2e-86	60.26	ribosome-inactivating protein bryodin II-like [Cucurbita maxima]	[more]

BLAST of Tan0005516 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E093	3.4e-95	64.00	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024726 PE=3 SV=1	[more]
A0A6J1EKL0	2.0e-87	60.20	rRNA N-glycosidase OS=Cucurbita moschata OX=3662 GN=LOC111433434 PE=3 SV=1	[more]
A0A6J1IUB2	5.8e-87	60.26	rRNA N-glycosidase OS=Cucurbita maxima OX=3661 GN=LOC111480641 PE=3 SV=1	[more]
A0A6J1DWK4	8.1e-81	67.73	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024728 PE=3 SV=1	[more]
A0A6J1DVE7	6.8e-80	67.33	rRNA N-glycosidase OS=Momordica charantia OX=3673 GN=LOC111024729 PE=3 SV=1	[more]

BLAST of Tan0005516 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR017989	Ribosome-inactivating protein type 1/2	PRINTS	PR00396	SHIGARICIN	coord: 45..58 score: 39.47 coord: 168..188 score: 59.65 coord: 138..152 score: 47.54 coord: 200..217 score: 45.76
IPR016139	Ribosome-inactivating protein, subdomain 2	GENE3D	4.10.470.10	Ricin (A Subunit), domain 2	coord: 180..263 e-value: 2.1E-25 score: 90.4
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 33..83 e-value: 6.5E-7 score: 31.1
IPR016138	Ribosome-inactivating protein, subdomain 1	GENE3D	3.40.420.10	Ricin (A subunit), domain 1	coord: 84..179 e-value: 1.2E-17 score: 66.1
IPR001574	Ribosome-inactivating protein	PFAM	PF00161	RIP	coord: 44..217 e-value: 3.7E-21 score: 75.7
IPR001574	Ribosome-inactivating protein	PANTHER	PTHR33453	FAMILY NOT NAMED	coord: 30..85 coord: 84..274
None	No IPR available	PANTHER	PTHR33453:SF14	RRNA N-GLYCOSIDASE	coord: 30..85 coord: 84..274
IPR036041	Ribosome-inactivating protein superfamily	SUPERFAMILY	56371	Ribosome inactivating proteins (RIP)	coord: 35..259

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0005516.1	Tan0005516.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0017148	negative regulation of translation
molecular_function	GO:0030598	rRNA N-glycosylase activity