Tan0005347 (gene) Snake gourd v1

Overview
NameTan0005347
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionCaM_binding domain-containing protein
LocationLG11: 3637233 .. 3641275 (-)
RNA-Seq ExpressionTan0005347
SyntenyTan0005347
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAAAACGCAACCGAACGGGGAAGAAATATTACAGTTGCTTGCACGACCCCTAAAGCGAATCGAAACCGACATTTTTGGTGCTCATTAAACAAATAATGGAATACAAAAGAGACGATCGACTCTAAAAGAGTGGACCCTTTTTTTCGCTTTCTCCCTATTTTGCTTCCACCTTCAGCGCCGTGCTCAATTCATTCATTTGTTTGTTCGTTCATTTTTCCTTGGCGTTTCGTTTTTCTTTGATTTCTGGTGATTGCAAGAGAGGGGCTGAAGATTTTCTTGCCCAACAACGAAAACCCACATAATTCGATTCTAAGTTGCGTCTCTTTGACGCGTTTTTTTTTATTCGGTAAGGAAATAAGGAATCATTTTGCTTAATCTCTGCAATGGGGTTGTGGTTTTCGTTCTTCTTTTCCCGTTTGGTTTGTAAATCATCGTTGTTTTCCTTTCATGCTGGTTTGTGTGGAGATTTGCCCCTTAAGAGTCTGTTTAATGGCTACGTCTTGTTTTTTTCTTCCTGTTTTACAATGTTGATTGCTGTGATGATTGATTATGTAAATGGGACTCCATTGGACCTATCTCTTATCTGATCTGGGTTGTGCTCTGTTTTCAATTTAAGATAAGTGTATTGGAATTGGAACGCATTGGCAATGTCTCCAATTATTATGTGTTGATTTTGTCAGCTTTCGTTGGTCTCCTTTCTTGGTTCTTCTCAAATGTTGAGTTTGTGTTAGCAGTATTGAGATGTTTTGGCCTGTGTGATTACCCTGTTCACGCTCTTGGCAGGATTCCAGGGACAACCTTTGTCTTCTTTCTCATGATTCCACACGGACTTTTGTATTTATGTGTTCCAATTATGTTGCTTCCTATGTTAAATGTGTTACTAAAATGCAAGTGTTTCATGGGCATTATTGATTCAGCATATTGTGAACAAAATGATTCTTTTTCTTAAAGTTCATCTTCATCTATGTTCATGAGATGATATGCGCTAACAATGGATTCATTCTTCCCTTGGATATAAATTGTTGCATTTTGATTTGTCCTGCCTCTTGTTTTGTTTTCTTTCCATTTATGTTTTTAATGTCTCGAAGGGTGACATGAATGTTTGATTAATGTAACGTTTTTCTATTTTGTTTCTAGCTTTTATCTTGGAAGAAACGCATGAGAATAAAATCCTTAAATGAGAGAAGGCTTCTTCTATAAACTCTTGTCCTTATATGTATGTGTCTTGAAGTTCTGTTGTCTCTCAAATTCAATTTTTGAGTTAAGGTGCATTATTTTGGATGATGGTAGTTGCTAGTCGGTGCCTCATATAGTTTTTTTTTTTTCCATCTTCTCACTTTTTGTTATATGTGCAGTGGATTGTATGGTCTGATACAATCTCTCTTATAATATTATAGCATGTCTGTTTGTATCCACTGTTAGTCCTCTGTCTTCCTGTAGTTTGTTCTATATATTTATTTATTTATTTCATATGATTCCTCTAATCGCTGACTGGTTTCTTTATTGGTTTCTTCAGAGTGAGATTTTGGTAGAAATTGTTTCGGACCATTGCAGTTTATCAAAAACTACCCTTGTGATAACACCTTGGTTGGTTTCTCTAATTCTTCGTGCTATTTTTTATTCTTTTTGTTAAGGCCTATTAAGTTTCTTGATTGGTGTGCTAATTGTTTTTGTTTAGGTGGTGTGTCAAATTCTGATGGCTGAGAAGGGGATGTGCCAGTGACCCTGGAAATGACTGTGACTGTGGGTACTAGTTCAAGAAGAAGATCTATGGGATGGATGAGTTCTTCAAATAGTGGAGAAGAATTTGTGCCTCATTATCTTAGAGCATCTACTGGTTCCTGTCATGATGTTTGCAAATATGGGAAAAACCATACTTTTGAAACCAAGTCAAGACAACCAATGCTCAAAATTTTGACAAGAAGATCACTTGACGGTGGAAGTTCGGTAGATAGTTTGGTACTTCCTGAGAGAAAGAAAACAGGTGGAAGTTTGGCAGATTGTGTAATAATTCCTGAGAGAAAGATAACAACTACGACCCACATGTCCAAAAATGTGACAAGAAAATCGCTCGATGGTGGAAGTTCGGTTGATAGTATGGTACTTCCAGAGAGAGAGAAAACAAGTTCGACTCGAATGCTAAAAAATGTGGCAAGAAAATCACTTGACGGTGGAAGTTCGGTAGATAGTGTGGTACTTCCTGAGAGAAAGAAAACAACTTTAACTCAAATGCCCAAAAATGTGGCAAGAAAATCATTTGGTGGTGGAAGTTCGGTAGATAGTGTCATACTTCCTGAGAGAAAGAAAACAACTTTGACTCGGGCCAAGGCTGAATTTTCATCAACCTCCAGAATATCTGAAGCTGATTTGACCACCTTCATAAGGCCTAAATTGTCAATAGAATCTTCCGGCTTCCCAAGACCAGTACAGAGGGAAGTCCTGAATGAAAGGAAGAAGAAACTGTTGCCAAGCCCAATGCAGAGAGCAGTCCTGAATGAAAGGAAGAAGAAATTGTTGGCTGAGCCGAGAACTTTATCCAAGCCAAGATCACATACAGCAAATGCCCTGAAAAACTTGAAGCCTGAAACTTCCTCAGCCACTAGAAATCCAGAAGACTCCGTAGTTCAAGTTGTTGCAAAAGCCAAGGAGAGAAAGTTACCTGAAAAGTCTGATCAAATTTCGAAATTAAAGTCTATTAAAGTAAAGCCATTGAGATCTGCTGTGTCTTCGGACAATTTGAGAAGAAAAAATGATTCCAAAACGGGAAAAAGTTTTGGGACCTCCAAAGTAGCTGCAAAGAAAGTGGTGGCAACCTCAACAGGTTCATTTTCCTCAAATTCTATCTATGGAACTGCAAACTTAATGGCAAGAAAGCGTGTTAACTTGAAAGGCGTCCCACTTAAAACTCATAACAAGATCAAAGTATCTGAACGTGAACAAGTCCAGAGTGAAGAGGTCGAAGAGAAAACCTTCCGGAGTGAAGAGGTCCAAGGGGAAAGCTTACAGAGTGAAGAGGTACAAGAGAAAACCTTGTATGTCATTAAGATAGAAAATGAGGAGATACCTCATCAATCTGATCAAAATGAAACTGATGATAACATGGAACCACTGCCATCATCACCACCTAAATCGCTATCACCACCAACATCACCGTATATTTCAGCCAATGTAGAAGATCAAGGTGTATCTGAATACACAGAAAACACAGAAAGTGAAGCACAGGATGACTCTTACTCTGAAAGCGATGAAATTGGTAGCATGGAAGCAGATAATGTTTCAAATGAAGGTGGTGAAAAAGGAAGATTCCAAAATCGTGGGATGTTGCAAACTAAAGAAAAGGATCCCCAATCTACAAAACTAAGTTTCAGGAGGGGAAAAATTATCGACATCCCTTCCGAGAGTAATAGCCCGAGGAGGCTTAAATTTAGGCATGGAAGGCTGTTGGGGGAGAGTCAAAGGGCTGCGGTTGGCCTTAGGAAAAACTTCAAGAGGGGAACAGAAGTTGACAGCGACACAGCAACAGCTCAAGAAACTGTTGTTCTGAGGCACCAAGATGTGCAGGGGAAAAAAGATGCACAGGGATTGTTTAACAATGTCATTGAAGAAACTGCAAGTAAACTTGTCGAAACTCGAAAGAGCAAGGTTAAGGCCTTGGTTGGTGCATTTGAAACAGTGATCTCCCTGCAAGATGGAAAACCTTCTCTTGAGTCTTGACACTGCCAGTTGAGTGAGAAATTGAGCTTGACTTATGAATCTAATTGATCTGCATAGAGTGGTAGGAAATAAACCTTGGGTTGCAAAGAGGATATGAACTCGACGAGCTGACACTGCAGTAGAGTCATAGGTATAGGTTGCTCTTTTTAATGATAGGAAGATTTCTGTACACAGTTAGATAGGAAGTATTGTTTGTGCTGATCTTTTTTCCCTAATCACCAATCGTTCTACTGAATTGATATTATTGATTTGTGAGTTGCACATAAAATAAGGGTAGGCTCATGACTTCATGTATTTTTGAATTGACAGTTATCTGCCAACTTTGGATC

mRNA sequence

GAAAACGCAACCGAACGGGGAAGAAATATTACAGTTGCTTGCACGACCCCTAAAGCGAATCGAAACCGACATTTTTGGTGCTCATTAAACAAATAATGGAATACAAAAGAGACGATCGACTCTAAAAGAGTGGACCCTTTTTTTCGCTTTCTCCCTATTTTGCTTCCACCTTCAGCGCCGTGCTCAATTCATTCATTTGTTTGTTCGTTCATTTTTCCTTGGCGTTTCGTTTTTCTTTGATTTCTGGTGATTGCAAGAGAGGGGCTGAAGATTTTCTTGCCCAACAACGAAAACCCACATAATTCGATTCTAAGTTGCGTCTCTTTGACGCGTTTTTTTTTATTCGAGTGAGATTTTGGTAGAAATTGTTTCGGACCATTGCAGTTTATCAAAAACTACCCTTGTGATAACACCTTGGTGGTGTGTCAAATTCTGATGGCTGAGAAGGGGATGTGCCAGTGACCCTGGAAATGACTGTGACTGTGGGTACTAGTTCAAGAAGAAGATCTATGGGATGGATGAGTTCTTCAAATAGTGGAGAAGAATTTGTGCCTCATTATCTTAGAGCATCTACTGGTTCCTGTCATGATGTTTGCAAATATGGGAAAAACCATACTTTTGAAACCAAGTCAAGACAACCAATGCTCAAAATTTTGACAAGAAGATCACTTGACGGTGGAAGTTCGGTAGATAGTTTGGTACTTCCTGAGAGAAAGAAAACAGGTGGAAGTTTGGCAGATTGTGTAATAATTCCTGAGAGAAAGATAACAACTACGACCCACATGTCCAAAAATGTGACAAGAAAATCGCTCGATGGTGGAAGTTCGGTTGATAGTATGGTACTTCCAGAGAGAGAGAAAACAAGTTCGACTCGAATGCTAAAAAATGTGGCAAGAAAATCACTTGACGGTGGAAGTTCGGTAGATAGTGTGGTACTTCCTGAGAGAAAGAAAACAACTTTAACTCAAATGCCCAAAAATGTGGCAAGAAAATCATTTGGTGGTGGAAGTTCGGTAGATAGTGTCATACTTCCTGAGAGAAAGAAAACAACTTTGACTCGGGCCAAGGCTGAATTTTCATCAACCTCCAGAATATCTGAAGCTGATTTGACCACCTTCATAAGGCCTAAATTGTCAATAGAATCTTCCGGCTTCCCAAGACCAGTACAGAGGGAAGTCCTGAATGAAAGGAAGAAGAAACTGTTGCCAAGCCCAATGCAGAGAGCAGTCCTGAATGAAAGGAAGAAGAAATTGTTGGCTGAGCCGAGAACTTTATCCAAGCCAAGATCACATACAGCAAATGCCCTGAAAAACTTGAAGCCTGAAACTTCCTCAGCCACTAGAAATCCAGAAGACTCCGTAGTTCAAGTTGTTGCAAAAGCCAAGGAGAGAAAGTTACCTGAAAAGTCTGATCAAATTTCGAAATTAAAGTCTATTAAAGTAAAGCCATTGAGATCTGCTGTGTCTTCGGACAATTTGAGAAGAAAAAATGATTCCAAAACGGGAAAAAGTTTTGGGACCTCCAAAGTAGCTGCAAAGAAAGTGGTGGCAACCTCAACAGGTTCATTTTCCTCAAATTCTATCTATGGAACTGCAAACTTAATGGCAAGAAAGCGTGTTAACTTGAAAGGCGTCCCACTTAAAACTCATAACAAGATCAAAGTATCTGAACGTGAACAAGTCCAGAGTGAAGAGGTCGAAGAGAAAACCTTCCGGAGTGAAGAGGTCCAAGGGGAAAGCTTACAGAGTGAAGAGGTACAAGAGAAAACCTTGTATGTCATTAAGATAGAAAATGAGGAGATACCTCATCAATCTGATCAAAATGAAACTGATGATAACATGGAACCACTGCCATCATCACCACCTAAATCGCTATCACCACCAACATCACCGTATATTTCAGCCAATGTAGAAGATCAAGGTGTATCTGAATACACAGAAAACACAGAAAGTGAAGCACAGGATGACTCTTACTCTGAAAGCGATGAAATTGGTAGCATGGAAGCAGATAATGTTTCAAATGAAGGTGGTGAAAAAGGAAGATTCCAAAATCGTGGGATGTTGCAAACTAAAGAAAAGGATCCCCAATCTACAAAACTAAGTTTCAGGAGGGGAAAAATTATCGACATCCCTTCCGAGAGTAATAGCCCGAGGAGGCTTAAATTTAGGCATGGAAGGCTGTTGGGGGAGAGTCAAAGGGCTGCGGTTGGCCTTAGGAAAAACTTCAAGAGGGGAACAGAAGTTGACAGCGACACAGCAACAGCTCAAGAAACTGTTGTTCTGAGGCACCAAGATGTGCAGGGGAAAAAAGATGCACAGGGATTGTTTAACAATGTCATTGAAGAAACTGCAAGTAAACTTGTCGAAACTCGAAAGAGCAAGGTTAAGGCCTTGGTTGGTGCATTTGAAACAGTGATCTCCCTGCAAGATGGAAAACCTTCTCTTGAGTCTTGACACTGCCAGTTGAGTGAGAAATTGAGCTTGACTTATGAATCTAATTGATCTGCATAGAGTGGTAGGAAATAAACCTTGGGTTGCAAAGAGGATATGAACTCGACGAGCTGACACTGCAGTAGAGTCATAGGTATAGGTTGCTCTTTTTAATGATAGGAAGATTTCTGTACACAGTTAGATAGGAAGTATTGTTTGTGCTGATCTTTTTTCCCTAATCACCAATCGTTCTACTGAATTGATATTATTGATTTGTGAGTTGCACATAAAATAAGGGTAGGCTCATGACTTCATGTATTTTTGAATTGACAGTTATCTGCCAACTTTGGATC

Coding sequence (CDS)

ATGACTGTGACTGTGGGTACTAGTTCAAGAAGAAGATCTATGGGATGGATGAGTTCTTCAAATAGTGGAGAAGAATTTGTGCCTCATTATCTTAGAGCATCTACTGGTTCCTGTCATGATGTTTGCAAATATGGGAAAAACCATACTTTTGAAACCAAGTCAAGACAACCAATGCTCAAAATTTTGACAAGAAGATCACTTGACGGTGGAAGTTCGGTAGATAGTTTGGTACTTCCTGAGAGAAAGAAAACAGGTGGAAGTTTGGCAGATTGTGTAATAATTCCTGAGAGAAAGATAACAACTACGACCCACATGTCCAAAAATGTGACAAGAAAATCGCTCGATGGTGGAAGTTCGGTTGATAGTATGGTACTTCCAGAGAGAGAGAAAACAAGTTCGACTCGAATGCTAAAAAATGTGGCAAGAAAATCACTTGACGGTGGAAGTTCGGTAGATAGTGTGGTACTTCCTGAGAGAAAGAAAACAACTTTAACTCAAATGCCCAAAAATGTGGCAAGAAAATCATTTGGTGGTGGAAGTTCGGTAGATAGTGTCATACTTCCTGAGAGAAAGAAAACAACTTTGACTCGGGCCAAGGCTGAATTTTCATCAACCTCCAGAATATCTGAAGCTGATTTGACCACCTTCATAAGGCCTAAATTGTCAATAGAATCTTCCGGCTTCCCAAGACCAGTACAGAGGGAAGTCCTGAATGAAAGGAAGAAGAAACTGTTGCCAAGCCCAATGCAGAGAGCAGTCCTGAATGAAAGGAAGAAGAAATTGTTGGCTGAGCCGAGAACTTTATCCAAGCCAAGATCACATACAGCAAATGCCCTGAAAAACTTGAAGCCTGAAACTTCCTCAGCCACTAGAAATCCAGAAGACTCCGTAGTTCAAGTTGTTGCAAAAGCCAAGGAGAGAAAGTTACCTGAAAAGTCTGATCAAATTTCGAAATTAAAGTCTATTAAAGTAAAGCCATTGAGATCTGCTGTGTCTTCGGACAATTTGAGAAGAAAAAATGATTCCAAAACGGGAAAAAGTTTTGGGACCTCCAAAGTAGCTGCAAAGAAAGTGGTGGCAACCTCAACAGGTTCATTTTCCTCAAATTCTATCTATGGAACTGCAAACTTAATGGCAAGAAAGCGTGTTAACTTGAAAGGCGTCCCACTTAAAACTCATAACAAGATCAAAGTATCTGAACGTGAACAAGTCCAGAGTGAAGAGGTCGAAGAGAAAACCTTCCGGAGTGAAGAGGTCCAAGGGGAAAGCTTACAGAGTGAAGAGGTACAAGAGAAAACCTTGTATGTCATTAAGATAGAAAATGAGGAGATACCTCATCAATCTGATCAAAATGAAACTGATGATAACATGGAACCACTGCCATCATCACCACCTAAATCGCTATCACCACCAACATCACCGTATATTTCAGCCAATGTAGAAGATCAAGGTGTATCTGAATACACAGAAAACACAGAAAGTGAAGCACAGGATGACTCTTACTCTGAAAGCGATGAAATTGGTAGCATGGAAGCAGATAATGTTTCAAATGAAGGTGGTGAAAAAGGAAGATTCCAAAATCGTGGGATGTTGCAAACTAAAGAAAAGGATCCCCAATCTACAAAACTAAGTTTCAGGAGGGGAAAAATTATCGACATCCCTTCCGAGAGTAATAGCCCGAGGAGGCTTAAATTTAGGCATGGAAGGCTGTTGGGGGAGAGTCAAAGGGCTGCGGTTGGCCTTAGGAAAAACTTCAAGAGGGGAACAGAAGTTGACAGCGACACAGCAACAGCTCAAGAAACTGTTGTTCTGAGGCACCAAGATGTGCAGGGGAAAAAAGATGCACAGGGATTGTTTAACAATGTCATTGAAGAAACTGCAAGTAAACTTGTCGAAACTCGAAAGAGCAAGGTTAAGGCCTTGGTTGGTGCATTTGAAACAGTGATCTCCCTGCAAGATGGAAAACCTTCTCTTGAGTCTTGA

Protein sequence

MTVTVGTSSRRRSMGWMSSSNSGEEFVPHYLRASTGSCHDVCKYGKNHTFETKSRQPMLKILTRRSLDGGSSVDSLVLPERKKTGGSLADCVIIPERKITTTTHMSKNVTRKSLDGGSSVDSMVLPEREKTSSTRMLKNVARKSLDGGSSVDSVVLPERKKTTLTQMPKNVARKSFGGGSSVDSVILPERKKTTLTRAKAEFSSTSRISEADLTTFIRPKLSIESSGFPRPVQREVLNERKKKLLPSPMQRAVLNERKKKLLAEPRTLSKPRSHTANALKNLKPETSSATRNPEDSVVQVVAKAKERKLPEKSDQISKLKSIKVKPLRSAVSSDNLRRKNDSKTGKSFGTSKVAAKKVVATSTGSFSSNSIYGTANLMARKRVNLKGVPLKTHNKIKVSEREQVQSEEVEEKTFRSEEVQGESLQSEEVQEKTLYVIKIENEEIPHQSDQNETDDNMEPLPSSPPKSLSPPTSPYISANVEDQGVSEYTENTESEAQDDSYSESDEIGSMEADNVSNEGGEKGRFQNRGMLQTKEKDPQSTKLSFRRGKIIDIPSESNSPRRLKFRHGRLLGESQRAAVGLRKNFKRGTEVDSDTATAQETVVLRHQDVQGKKDAQGLFNNVIEETASKLVETRKSKVKALVGAFETVISLQDGKPSLES
Homology
BLAST of Tan0005347 vs. NCBI nr
Match: XP_022990927.1 (uncharacterized protein LOC111487671 isoform X2 [Cucurbita maxima])

HSP 1 Score: 827.0 bits (2135), Expect = 1.1e-235
Identity = 490/687 (71.32%), Postives = 540/687 (78.60%), Query Frame = 0

Query: 1   MTVTVGTSSRRRSMGWMSSSNSGEEFVPHYLRASTGSCHDVCKYGKNHTFETKSRQPMLK 60
           MT T GTSSRR S GW+SSSNSGE+FVPHY R STGSCHD+CKYGKNHTFETKSRQP+  
Sbjct: 10  MTTTAGTSSRRSSTGWISSSNSGEKFVPHYRRISTGSCHDICKYGKNHTFETKSRQPV-- 69

Query: 61  ILTRRSLDGGSSVDSLVLPERKKTGGSLADCVIIPERKITTTTHMSKNVTRKSLDGGSSV 120
            +TR+SLDGG SVD +VL ERKKTGG   D V+IPERK T  T  SK++TRKSLD GSSV
Sbjct: 70  AMTRKSLDGGRSVDDVVLRERKKTGGGSVDSVVIPERKKTIMTERSKHMTRKSLDSGSSV 129

Query: 121 DSMVLPEREKTSSTRMLKNVARKSLDGGSSVDSVVLPERKKTTLTQMPKNVARKSFGG-- 180
           DSMVLPER+KT+STRM KNVAR SLDGGSSVD VVL ERKKT   +M KNVAR S  G  
Sbjct: 130 DSMVLPERKKTTSTRMSKNVARISLDGGSSVDGVVLLERKKTASPRMSKNVARSSIDGVV 189

Query: 181 -----------------------GSSVDSVILPERKKTTLTRAKAEFSSTSRISEADLTT 240
                                  GSSV+  ILPERKKT  TR KAE SSTSR+S A++TT
Sbjct: 190 LLERKKTISTRMSKNVGRISLDSGSSVNRTILPERKKTASTRTKAELSSTSRMSGANVTT 249

Query: 241 FIRPKLSIESSGFPRPVQREVLNERKKKLLPSPMQRAVLNERKKKLLAEPRTLSKPRSHT 300
             +P           PVQREV N RKKKLL SP  R VLNERK KLLAEP+ L+K RSHT
Sbjct: 250 LTKPS----------PVQREVSNGRKKKLLSSPKLREVLNERKNKLLAEPKALAKSRSHT 309

Query: 301 ANALKNLKPETSSATRNPEDSVVQVVAKAKERKLPEKSDQISKLKSIKVKPLRSAVSSDN 360
           AN LKN KPETS+ATRNPEDS   V+AK KERKLPEKS+   KLKSIKV PLRSAVS+D 
Sbjct: 310 ANMLKNSKPETSTATRNPEDSA--VLAKTKERKLPEKSENFVKLKSIKVNPLRSAVSTDG 369

Query: 361 LRRKNDSKTGKSFGTSKVAAKKVVATSTGSFSSNSIYGTANLMARKRVNLKGVPLKTHNK 420
            RR N SK  K+FGTSKVAAKKVVATSTGSFSSNSIYG ANL ARK V+LKG   KTHNK
Sbjct: 370 SRRTNGSKMVKNFGTSKVAAKKVVATSTGSFSSNSIYGNANLTARKHVSLKGGLHKTHNK 429

Query: 421 IKVSEREQVQSEEVEEKTFRSEEVQGESLQSEEVQEKTLYVIKIENEEIPHQSD-QNETD 480
            K+S+ E+VQSEEV  +TF+SEEVQGE+ QS EVQEKTLYVIK+ENEE+PHQSD QNET 
Sbjct: 430 NKLSDHERVQSEEVRGETFQSEEVQGETFQSVEVQEKTLYVIKVENEEMPHQSDGQNETF 489

Query: 481 DNMEPLPSSPPKSLSPPTSPYISANVEDQGVSEYTENTESEAQDDSYSESDEIGSMEADN 540
           DNMEPLPSS   SLSPP++ YI ANV+DQ VSEY   TESE ++DS+SES+E GSME DN
Sbjct: 490 DNMEPLPSSRLNSLSPPSAQYILANVKDQDVSEY---TESETENDSFSESNEYGSMETDN 549

Query: 541 VSN-EGGEKGRFQNRGMLQTKEKDPQSTKLSFRRGKIIDIPSESNSPRRLKFRHGRLLGE 600
            S+ EGG+  R+QN  +L  KE+DPQSTKLSFRRGKIIDIP++SNSPRRLKFR GRLLGE
Sbjct: 550 ASSEEGGQNARYQNPRILHNKEEDPQSTKLSFRRGKIIDIPAKSNSPRRLKFRRGRLLGE 609

Query: 601 SQRAAVGLRKNFKRGTEVDSDTATAQETVVLRHQDV-QGKKDAQGLFNNVIEETASKLVE 660
           SQR A GLRKNFK GTEVD DTATAQE VVLRHQDV Q +KDAQGLFNNVIEETASKLVE
Sbjct: 610 SQRDADGLRKNFKMGTEVDGDTATAQEIVVLRHQDVQQRRKDAQGLFNNVIEETASKLVE 669

BLAST of Tan0005347 vs. NCBI nr
Match: XP_022990925.1 (uncharacterized protein LOC111487671 isoform X1 [Cucurbita maxima] >XP_022990926.1 uncharacterized protein LOC111487671 isoform X1 [Cucurbita maxima])

HSP 1 Score: 824.3 bits (2128), Expect = 7.4e-235
Identity = 492/697 (70.59%), Postives = 542/697 (77.76%), Query Frame = 0

Query: 1   MTVTVGTSSRRRSMGWMSSSNSGEEFVPHYLRASTGSCHDVCKYGKNHTFETKSRQPMLK 60
           MT T GTSSRR S GW+SSSNSGE+FVPHY R STGSCHD+CKYGKNHTFETKSRQP+  
Sbjct: 10  MTTTAGTSSRRSSTGWISSSNSGEKFVPHYRRISTGSCHDICKYGKNHTFETKSRQPV-- 69

Query: 61  ILTRRSLDGGSSVDSLVLPERKKTGGSLADCVIIPERKITTTTHMSKNVTRKSLDGGSSV 120
            +TR+SLDGG SVD +VL ERKKTGG   D V+IPERK T  T  SK++TRKSLD GSSV
Sbjct: 70  AMTRKSLDGGRSVDDVVLRERKKTGGGSVDSVVIPERKKTIMTERSKHMTRKSLDSGSSV 129

Query: 121 DSMVLPEREKTSSTRMLKNVARKSLDGGSSVDSVVLPERKKTTLTQMPKNVARKSFGG-- 180
           DSMVLPER+KT+STRM KNVAR SLDGGSSVD VVL ERKKT   +M KNVAR S  G  
Sbjct: 130 DSMVLPERKKTTSTRMSKNVARISLDGGSSVDGVVLLERKKTASPRMSKNVARSSIDGVV 189

Query: 181 -----------------------GSSVDSVILPERKKTTLTRAKAEFSSTSRISEADLTT 240
                                  GSSV+  ILPERKKT  TR KAE SSTSR+S A++TT
Sbjct: 190 LLERKKTISTRMSKNVGRISLDSGSSVNRTILPERKKTASTRTKAELSSTSRMSGANVTT 249

Query: 241 FIRPKLSIESSGFPRPVQREVLNERKKKLLPSPMQRAVLNERKKKLLAEPRTLSKPRSHT 300
             +P           PVQREV N RKKKLL SP  R VLNERK KLLAEP+ L+K RSHT
Sbjct: 250 LTKPS----------PVQREVSNGRKKKLLSSPKLREVLNERKNKLLAEPKALAKSRSHT 309

Query: 301 ANALKNLKPETSSATRNPEDSVVQVVAKAKERKLPEKSDQISKLKSIKVKPLRSAVSSDN 360
           AN LKN KPETS+ATRNPEDS   V+AK KERKLPEKS+   KLKSIKV PLRSAVS+D 
Sbjct: 310 ANMLKNSKPETSTATRNPEDSA--VLAKTKERKLPEKSENFVKLKSIKVNPLRSAVSTDG 369

Query: 361 LRRKNDSKTGKSFGTSKVAAKKVVATSTGSFSSNSIYGTANLMARKRVNLKGVPLKTHNK 420
            RR N SK  K+FGTSKVAAKKVVATSTGSFSSNSIYG ANL ARK V+LKG   KTHNK
Sbjct: 370 SRRTNGSKMVKNFGTSKVAAKKVVATSTGSFSSNSIYGNANLTARKHVSLKGGLHKTHNK 429

Query: 421 IKVSEREQVQSEEVEEKTFRSEEVQGESLQSE----------EVQEKTLYVIKIENEEIP 480
            K+S+ E+VQSEEV +KTFRSEEV+GE+ QSE          EVQEKTLYVIK+ENEE+P
Sbjct: 430 NKLSDHERVQSEEVNKKTFRSEEVRGETFQSEEVQGETFQSVEVQEKTLYVIKVENEEMP 489

Query: 481 HQSD-QNETDDNMEPLPSSPPKSLSPPTSPYISANVEDQGVSEYTENTESEAQDDSYSES 540
           HQSD QNET DNMEPLPSS   SLSPP++ YI ANV+DQ VSEY   TESE ++DS+SES
Sbjct: 490 HQSDGQNETFDNMEPLPSSRLNSLSPPSAQYILANVKDQDVSEY---TESETENDSFSES 549

Query: 541 DEIGSMEADNVSN-EGGEKGRFQNRGMLQTKEKDPQSTKLSFRRGKIIDIPSESNSPRRL 600
           +E GSME DN S+ EGG+  R+QN  +L  KE+DPQSTKLSFRRGKIIDIP++SNSPRRL
Sbjct: 550 NEYGSMETDNASSEEGGQNARYQNPRILHNKEEDPQSTKLSFRRGKIIDIPAKSNSPRRL 609

Query: 601 KFRHGRLLGESQRAAVGLRKNFKRGTEVDSDTATAQETVVLRHQDV-QGKKDAQGLFNNV 660
           KFR GRLLGESQR A GLRKNFK GTEVD DTATAQE VVLRHQDV Q +KDAQGLFNNV
Sbjct: 610 KFRRGRLLGESQRDADGLRKNFKMGTEVDGDTATAQEIVVLRHQDVQQRRKDAQGLFNNV 669

BLAST of Tan0005347 vs. NCBI nr
Match: XP_023541286.1 (uncharacterized protein LOC111801497 isoform X2 [Cucurbita pepo subsp. pepo] >XP_023541294.1 uncharacterized protein LOC111801497 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 815.8 bits (2106), Expect = 2.6e-232
Identity = 491/712 (68.96%), Postives = 540/712 (75.84%), Query Frame = 0

Query: 1   MTVTVGTSSRRRSMGWMSSSNSGEEFVPHYLRASTGSCHDVCKYGKNHTFETKSRQPMLK 60
           MT T GTSSRR S GW+SSSNSGE+FVPHY R STGSCHD CKYGKNHTFETKSR+PM  
Sbjct: 10  MTTTAGTSSRRSSTGWISSSNSGEKFVPHYRRISTGSCHDFCKYGKNHTFETKSRRPM-- 69

Query: 61  ILTRRSLDGGSSVDSLVLPERKKTGGSLADCVIIPERKITTTTHMSKNVTRKSLDGGSSV 120
            +TR+SLDGG S DS+VL ERKKTGG   D V+IPERK T  T  SK++TRKSLD GSSV
Sbjct: 70  AMTRKSLDGGRSEDSVVLRERKKTGGGSVDGVVIPERKKTIMTERSKHMTRKSLDDGSSV 129

Query: 121 DSMVLPEREKTSSTRMLKNVARK-------------------------SLDGGSSVDSVV 180
           DSMVLPER+KT+S RM KNVARK                         SLDGGSSVD VV
Sbjct: 130 DSMVLPERKKTTSPRMSKNVARKSLDGVVLLERKKTTSTRMSKNVARISLDGGSSVDGVV 189

Query: 181 LPERKKTTLTQMPKNVARKSFGG-------------------------GSSVDSVILPER 240
           L ERKKT   +M KNVAR S  G                         GSS+   ILPER
Sbjct: 190 LLERKKTASPRMSKNVARSSIDGVVLLERKKTISTRMLKNVARISLDSGSSI-RTILPER 249

Query: 241 KKTTLTRAKAEFSSTSRISEADLTTFIRPKLSIESSGFPRPVQREVLNERKKKLLPSPMQ 300
           KKT  TR KAE SSTSR+S A++TT  RP           PVQREV N RKKKLL SP Q
Sbjct: 250 KKTASTRMKAELSSTSRMSGANVTTLTRPS----------PVQREVSNGRKKKLLSSPTQ 309

Query: 301 RAVLNERKKKLLAEPRTLSKPRSHTANALKNLKPETSSATRNPEDSVVQVVAKAKERKLP 360
           R VLNERKKKLLAEP+ L+K RSHTAN L N KPETS+ATRNPEDS   V+AK KERKLP
Sbjct: 310 REVLNERKKKLLAEPKALAKSRSHTANTLNNSKPETSTATRNPEDSA--VLAKTKERKLP 369

Query: 361 EKSDQISKLKSIKVKPLRSAVSSDNLRRKNDSKTGKSFGTSKVAAKKVVATSTGSFSSNS 420
           EKS+   KL+SIKV PLRSAVS+D  RR N SK  K+ GTSKVAAKKVVATSTGSFSSNS
Sbjct: 370 EKSENFVKLRSIKVNPLRSAVSTDGSRRTNGSKMVKNLGTSKVAAKKVVATSTGSFSSNS 429

Query: 421 IYGTANLMARKRVNLKGVPLKTHNKIKVSEREQVQSEEVEEKTFRSEEVQGESLQSEEVQ 480
           IYG ANL ARKRVNLKG P KTHNK K+S+ +QVQ EEV  +TF+SEEVQGE+ +S EVQ
Sbjct: 430 IYGNANLTARKRVNLKGSPHKTHNKNKLSDHQQVQGEEVRGETFQSEEVQGETFRSVEVQ 489

Query: 481 EKTLYVIKIENEEIPHQSD-QNETDDNMEPLPSSPPKSLSPPTSPYISANVEDQGVSEYT 540
           EKTLYVIK+ENEE+PH+ D QNET DN+EPLPSSPP SLSPP++ YI A V+DQ VSEY 
Sbjct: 490 EKTLYVIKVENEEMPHEPDGQNETFDNVEPLPSSPPNSLSPPSAQYILAKVKDQDVSEY- 549

Query: 541 ENTESEAQDDSYSESDEIGSMEADNVSN-EGGEKGRFQNRGMLQTKEKDPQSTKLSFRRG 600
             TESE ++DS+SES+E GSME DNVS+ EGG+  R+QN  ML  KE+DPQSTKLSFRRG
Sbjct: 550 --TESETENDSFSESNEYGSMETDNVSSEEGGQNARYQNPRMLHNKEEDPQSTKLSFRRG 609

Query: 601 KIIDIPSESNSPRRLKFRHGRLLGESQRAAVGLRKNFKRGTEVDSDTATAQETVVLRHQD 660
           KIIDIPS+SNSPRRLKFR GRLLGESQR A GLRKNFK GTEVD DTATAQE VVLRHQD
Sbjct: 610 KIIDIPSKSNSPRRLKFRRGRLLGESQRDAEGLRKNFKMGTEVDGDTATAQEIVVLRHQD 669

BLAST of Tan0005347 vs. NCBI nr
Match: XP_023541278.1 (uncharacterized protein LOC111801497 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 815.8 bits (2106), Expect = 2.6e-232
Identity = 491/712 (68.96%), Postives = 540/712 (75.84%), Query Frame = 0

Query: 1   MTVTVGTSSRRRSMGWMSSSNSGEEFVPHYLRASTGSCHDVCKYGKNHTFETKSRQPMLK 60
           MT T GTSSRR S GW+SSSNSGE+FVPHY R STGSCHD CKYGKNHTFETKSR+PM  
Sbjct: 32  MTTTAGTSSRRSSTGWISSSNSGEKFVPHYRRISTGSCHDFCKYGKNHTFETKSRRPM-- 91

Query: 61  ILTRRSLDGGSSVDSLVLPERKKTGGSLADCVIIPERKITTTTHMSKNVTRKSLDGGSSV 120
            +TR+SLDGG S DS+VL ERKKTGG   D V+IPERK T  T  SK++TRKSLD GSSV
Sbjct: 92  AMTRKSLDGGRSEDSVVLRERKKTGGGSVDGVVIPERKKTIMTERSKHMTRKSLDDGSSV 151

Query: 121 DSMVLPEREKTSSTRMLKNVARK-------------------------SLDGGSSVDSVV 180
           DSMVLPER+KT+S RM KNVARK                         SLDGGSSVD VV
Sbjct: 152 DSMVLPERKKTTSPRMSKNVARKSLDGVVLLERKKTTSTRMSKNVARISLDGGSSVDGVV 211

Query: 181 LPERKKTTLTQMPKNVARKSFGG-------------------------GSSVDSVILPER 240
           L ERKKT   +M KNVAR S  G                         GSS+   ILPER
Sbjct: 212 LLERKKTASPRMSKNVARSSIDGVVLLERKKTISTRMLKNVARISLDSGSSI-RTILPER 271

Query: 241 KKTTLTRAKAEFSSTSRISEADLTTFIRPKLSIESSGFPRPVQREVLNERKKKLLPSPMQ 300
           KKT  TR KAE SSTSR+S A++TT  RP           PVQREV N RKKKLL SP Q
Sbjct: 272 KKTASTRMKAELSSTSRMSGANVTTLTRPS----------PVQREVSNGRKKKLLSSPTQ 331

Query: 301 RAVLNERKKKLLAEPRTLSKPRSHTANALKNLKPETSSATRNPEDSVVQVVAKAKERKLP 360
           R VLNERKKKLLAEP+ L+K RSHTAN L N KPETS+ATRNPEDS   V+AK KERKLP
Sbjct: 332 REVLNERKKKLLAEPKALAKSRSHTANTLNNSKPETSTATRNPEDSA--VLAKTKERKLP 391

Query: 361 EKSDQISKLKSIKVKPLRSAVSSDNLRRKNDSKTGKSFGTSKVAAKKVVATSTGSFSSNS 420
           EKS+   KL+SIKV PLRSAVS+D  RR N SK  K+ GTSKVAAKKVVATSTGSFSSNS
Sbjct: 392 EKSENFVKLRSIKVNPLRSAVSTDGSRRTNGSKMVKNLGTSKVAAKKVVATSTGSFSSNS 451

Query: 421 IYGTANLMARKRVNLKGVPLKTHNKIKVSEREQVQSEEVEEKTFRSEEVQGESLQSEEVQ 480
           IYG ANL ARKRVNLKG P KTHNK K+S+ +QVQ EEV  +TF+SEEVQGE+ +S EVQ
Sbjct: 452 IYGNANLTARKRVNLKGSPHKTHNKNKLSDHQQVQGEEVRGETFQSEEVQGETFRSVEVQ 511

Query: 481 EKTLYVIKIENEEIPHQSD-QNETDDNMEPLPSSPPKSLSPPTSPYISANVEDQGVSEYT 540
           EKTLYVIK+ENEE+PH+ D QNET DN+EPLPSSPP SLSPP++ YI A V+DQ VSEY 
Sbjct: 512 EKTLYVIKVENEEMPHEPDGQNETFDNVEPLPSSPPNSLSPPSAQYILAKVKDQDVSEY- 571

Query: 541 ENTESEAQDDSYSESDEIGSMEADNVSN-EGGEKGRFQNRGMLQTKEKDPQSTKLSFRRG 600
             TESE ++DS+SES+E GSME DNVS+ EGG+  R+QN  ML  KE+DPQSTKLSFRRG
Sbjct: 572 --TESETENDSFSESNEYGSMETDNVSSEEGGQNARYQNPRMLHNKEEDPQSTKLSFRRG 631

Query: 601 KIIDIPSESNSPRRLKFRHGRLLGESQRAAVGLRKNFKRGTEVDSDTATAQETVVLRHQD 660
           KIIDIPS+SNSPRRLKFR GRLLGESQR A GLRKNFK GTEVD DTATAQE VVLRHQD
Sbjct: 632 KIIDIPSKSNSPRRLKFRRGRLLGESQRDAEGLRKNFKMGTEVDGDTATAQEIVVLRHQD 691

BLAST of Tan0005347 vs. NCBI nr
Match: XP_022990929.1 (uncharacterized protein LOC111487671 isoform X3 [Cucurbita maxima])

HSP 1 Score: 800.0 bits (2065), Expect = 1.5e-227
Identity = 480/697 (68.87%), Postives = 531/697 (76.18%), Query Frame = 0

Query: 1   MTVTVGTSSRRRSMGWMSSSNSGEEFVPHYLRASTGSCHDVCKYGKNHTFETKSRQPMLK 60
           MT T GTSSRR S GW+SSSNSGE+FVPHY R STGSCHD+CKYGKNHTFETKSRQP+  
Sbjct: 10  MTTTAGTSSRRSSTGWISSSNSGEKFVPHYRRISTGSCHDICKYGKNHTFETKSRQPV-- 69

Query: 61  ILTRRSLDGGSSVDSLVLPERKKTGGSLADCVIIPERKITTTTHMSKNVTRKSLDGGSSV 120
            +TR+SLDGG SVD +VL ERKKTGG   D V+IPERK T  T  SK++TRKSLD GSSV
Sbjct: 70  AMTRKSLDGGRSVDDVVLRERKKTGGGSVDSVVIPERKKTIMTERSKHMTRKSLDSGSSV 129

Query: 121 DSMVLPEREKTSSTRMLKNVARKSLDGGSSVDSVVLPERKKTTLTQMPKNVARKSFGG-- 180
           DSMVLPER+KT+STRM KNVAR SLDGGSSVD VVL ERKKT   +M KNVAR S  G  
Sbjct: 130 DSMVLPERKKTTSTRMSKNVARISLDGGSSVDGVVLLERKKTASPRMSKNVARSSIDGVV 189

Query: 181 -----------------------GSSVDSVILPERKKTTLTRAKAEFSSTSRISEADLTT 240
                                  GSSV+  ILPERKKT  TR KAE SSTSR+S A++TT
Sbjct: 190 LLERKKTISTRMSKNVGRISLDSGSSVNRTILPERKKTASTRTKAELSSTSRMSGANVTT 249

Query: 241 FIRPKLSIESSGFPRPVQREVLNERKKKLLPSPMQRAVLNERKKKLLAEPRTLSKPRSHT 300
             +                           PSP+QR VLNERK KLLAEP+ L+K RSHT
Sbjct: 250 LTK---------------------------PSPVQREVLNERKNKLLAEPKALAKSRSHT 309

Query: 301 ANALKNLKPETSSATRNPEDSVVQVVAKAKERKLPEKSDQISKLKSIKVKPLRSAVSSDN 360
           AN LKN KPETS+ATRNPEDS   V+AK KERKLPEKS+   KLKSIKV PLRSAVS+D 
Sbjct: 310 ANMLKNSKPETSTATRNPEDSA--VLAKTKERKLPEKSENFVKLKSIKVNPLRSAVSTDG 369

Query: 361 LRRKNDSKTGKSFGTSKVAAKKVVATSTGSFSSNSIYGTANLMARKRVNLKGVPLKTHNK 420
            RR N SK  K+FGTSKVAAKKVVATSTGSFSSNSIYG ANL ARK V+LKG   KTHNK
Sbjct: 370 SRRTNGSKMVKNFGTSKVAAKKVVATSTGSFSSNSIYGNANLTARKHVSLKGGLHKTHNK 429

Query: 421 IKVSEREQVQSEEVEEKTFRSEEVQGESLQSE----------EVQEKTLYVIKIENEEIP 480
            K+S+ E+VQSEEV +KTFRSEEV+GE+ QSE          EVQEKTLYVIK+ENEE+P
Sbjct: 430 NKLSDHERVQSEEVNKKTFRSEEVRGETFQSEEVQGETFQSVEVQEKTLYVIKVENEEMP 489

Query: 481 HQSD-QNETDDNMEPLPSSPPKSLSPPTSPYISANVEDQGVSEYTENTESEAQDDSYSES 540
           HQSD QNET DNMEPLPSS   SLSPP++ YI ANV+DQ VSEY   TESE ++DS+SES
Sbjct: 490 HQSDGQNETFDNMEPLPSSRLNSLSPPSAQYILANVKDQDVSEY---TESETENDSFSES 549

Query: 541 DEIGSMEADNVSN-EGGEKGRFQNRGMLQTKEKDPQSTKLSFRRGKIIDIPSESNSPRRL 600
           +E GSME DN S+ EGG+  R+QN  +L  KE+DPQSTKLSFRRGKIIDIP++SNSPRRL
Sbjct: 550 NEYGSMETDNASSEEGGQNARYQNPRILHNKEEDPQSTKLSFRRGKIIDIPAKSNSPRRL 609

Query: 601 KFRHGRLLGESQRAAVGLRKNFKRGTEVDSDTATAQETVVLRHQDV-QGKKDAQGLFNNV 660
           KFR GRLLGESQR A GLRKNFK GTEVD DTATAQE VVLRHQDV Q +KDAQGLFNNV
Sbjct: 610 KFRRGRLLGESQRDADGLRKNFKMGTEVDGDTATAQEIVVLRHQDVQQRRKDAQGLFNNV 669

BLAST of Tan0005347 vs. ExPASy TrEMBL
Match: A0A6J1JK93 (uncharacterized protein LOC111487671 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111487671 PE=4 SV=1)

HSP 1 Score: 827.0 bits (2135), Expect = 5.5e-236
Identity = 490/687 (71.32%), Postives = 540/687 (78.60%), Query Frame = 0

Query: 1   MTVTVGTSSRRRSMGWMSSSNSGEEFVPHYLRASTGSCHDVCKYGKNHTFETKSRQPMLK 60
           MT T GTSSRR S GW+SSSNSGE+FVPHY R STGSCHD+CKYGKNHTFETKSRQP+  
Sbjct: 10  MTTTAGTSSRRSSTGWISSSNSGEKFVPHYRRISTGSCHDICKYGKNHTFETKSRQPV-- 69

Query: 61  ILTRRSLDGGSSVDSLVLPERKKTGGSLADCVIIPERKITTTTHMSKNVTRKSLDGGSSV 120
            +TR+SLDGG SVD +VL ERKKTGG   D V+IPERK T  T  SK++TRKSLD GSSV
Sbjct: 70  AMTRKSLDGGRSVDDVVLRERKKTGGGSVDSVVIPERKKTIMTERSKHMTRKSLDSGSSV 129

Query: 121 DSMVLPEREKTSSTRMLKNVARKSLDGGSSVDSVVLPERKKTTLTQMPKNVARKSFGG-- 180
           DSMVLPER+KT+STRM KNVAR SLDGGSSVD VVL ERKKT   +M KNVAR S  G  
Sbjct: 130 DSMVLPERKKTTSTRMSKNVARISLDGGSSVDGVVLLERKKTASPRMSKNVARSSIDGVV 189

Query: 181 -----------------------GSSVDSVILPERKKTTLTRAKAEFSSTSRISEADLTT 240
                                  GSSV+  ILPERKKT  TR KAE SSTSR+S A++TT
Sbjct: 190 LLERKKTISTRMSKNVGRISLDSGSSVNRTILPERKKTASTRTKAELSSTSRMSGANVTT 249

Query: 241 FIRPKLSIESSGFPRPVQREVLNERKKKLLPSPMQRAVLNERKKKLLAEPRTLSKPRSHT 300
             +P           PVQREV N RKKKLL SP  R VLNERK KLLAEP+ L+K RSHT
Sbjct: 250 LTKPS----------PVQREVSNGRKKKLLSSPKLREVLNERKNKLLAEPKALAKSRSHT 309

Query: 301 ANALKNLKPETSSATRNPEDSVVQVVAKAKERKLPEKSDQISKLKSIKVKPLRSAVSSDN 360
           AN LKN KPETS+ATRNPEDS   V+AK KERKLPEKS+   KLKSIKV PLRSAVS+D 
Sbjct: 310 ANMLKNSKPETSTATRNPEDSA--VLAKTKERKLPEKSENFVKLKSIKVNPLRSAVSTDG 369

Query: 361 LRRKNDSKTGKSFGTSKVAAKKVVATSTGSFSSNSIYGTANLMARKRVNLKGVPLKTHNK 420
            RR N SK  K+FGTSKVAAKKVVATSTGSFSSNSIYG ANL ARK V+LKG   KTHNK
Sbjct: 370 SRRTNGSKMVKNFGTSKVAAKKVVATSTGSFSSNSIYGNANLTARKHVSLKGGLHKTHNK 429

Query: 421 IKVSEREQVQSEEVEEKTFRSEEVQGESLQSEEVQEKTLYVIKIENEEIPHQSD-QNETD 480
            K+S+ E+VQSEEV  +TF+SEEVQGE+ QS EVQEKTLYVIK+ENEE+PHQSD QNET 
Sbjct: 430 NKLSDHERVQSEEVRGETFQSEEVQGETFQSVEVQEKTLYVIKVENEEMPHQSDGQNETF 489

Query: 481 DNMEPLPSSPPKSLSPPTSPYISANVEDQGVSEYTENTESEAQDDSYSESDEIGSMEADN 540
           DNMEPLPSS   SLSPP++ YI ANV+DQ VSEY   TESE ++DS+SES+E GSME DN
Sbjct: 490 DNMEPLPSSRLNSLSPPSAQYILANVKDQDVSEY---TESETENDSFSESNEYGSMETDN 549

Query: 541 VSN-EGGEKGRFQNRGMLQTKEKDPQSTKLSFRRGKIIDIPSESNSPRRLKFRHGRLLGE 600
            S+ EGG+  R+QN  +L  KE+DPQSTKLSFRRGKIIDIP++SNSPRRLKFR GRLLGE
Sbjct: 550 ASSEEGGQNARYQNPRILHNKEEDPQSTKLSFRRGKIIDIPAKSNSPRRLKFRRGRLLGE 609

Query: 601 SQRAAVGLRKNFKRGTEVDSDTATAQETVVLRHQDV-QGKKDAQGLFNNVIEETASKLVE 660
           SQR A GLRKNFK GTEVD DTATAQE VVLRHQDV Q +KDAQGLFNNVIEETASKLVE
Sbjct: 610 SQRDADGLRKNFKMGTEVDGDTATAQEIVVLRHQDVQQRRKDAQGLFNNVIEETASKLVE 669

BLAST of Tan0005347 vs. ExPASy TrEMBL
Match: A0A6J1JPA7 (uncharacterized protein LOC111487671 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111487671 PE=4 SV=1)

HSP 1 Score: 824.3 bits (2128), Expect = 3.6e-235
Identity = 492/697 (70.59%), Postives = 542/697 (77.76%), Query Frame = 0

Query: 1   MTVTVGTSSRRRSMGWMSSSNSGEEFVPHYLRASTGSCHDVCKYGKNHTFETKSRQPMLK 60
           MT T GTSSRR S GW+SSSNSGE+FVPHY R STGSCHD+CKYGKNHTFETKSRQP+  
Sbjct: 10  MTTTAGTSSRRSSTGWISSSNSGEKFVPHYRRISTGSCHDICKYGKNHTFETKSRQPV-- 69

Query: 61  ILTRRSLDGGSSVDSLVLPERKKTGGSLADCVIIPERKITTTTHMSKNVTRKSLDGGSSV 120
            +TR+SLDGG SVD +VL ERKKTGG   D V+IPERK T  T  SK++TRKSLD GSSV
Sbjct: 70  AMTRKSLDGGRSVDDVVLRERKKTGGGSVDSVVIPERKKTIMTERSKHMTRKSLDSGSSV 129

Query: 121 DSMVLPEREKTSSTRMLKNVARKSLDGGSSVDSVVLPERKKTTLTQMPKNVARKSFGG-- 180
           DSMVLPER+KT+STRM KNVAR SLDGGSSVD VVL ERKKT   +M KNVAR S  G  
Sbjct: 130 DSMVLPERKKTTSTRMSKNVARISLDGGSSVDGVVLLERKKTASPRMSKNVARSSIDGVV 189

Query: 181 -----------------------GSSVDSVILPERKKTTLTRAKAEFSSTSRISEADLTT 240
                                  GSSV+  ILPERKKT  TR KAE SSTSR+S A++TT
Sbjct: 190 LLERKKTISTRMSKNVGRISLDSGSSVNRTILPERKKTASTRTKAELSSTSRMSGANVTT 249

Query: 241 FIRPKLSIESSGFPRPVQREVLNERKKKLLPSPMQRAVLNERKKKLLAEPRTLSKPRSHT 300
             +P           PVQREV N RKKKLL SP  R VLNERK KLLAEP+ L+K RSHT
Sbjct: 250 LTKPS----------PVQREVSNGRKKKLLSSPKLREVLNERKNKLLAEPKALAKSRSHT 309

Query: 301 ANALKNLKPETSSATRNPEDSVVQVVAKAKERKLPEKSDQISKLKSIKVKPLRSAVSSDN 360
           AN LKN KPETS+ATRNPEDS   V+AK KERKLPEKS+   KLKSIKV PLRSAVS+D 
Sbjct: 310 ANMLKNSKPETSTATRNPEDSA--VLAKTKERKLPEKSENFVKLKSIKVNPLRSAVSTDG 369

Query: 361 LRRKNDSKTGKSFGTSKVAAKKVVATSTGSFSSNSIYGTANLMARKRVNLKGVPLKTHNK 420
            RR N SK  K+FGTSKVAAKKVVATSTGSFSSNSIYG ANL ARK V+LKG   KTHNK
Sbjct: 370 SRRTNGSKMVKNFGTSKVAAKKVVATSTGSFSSNSIYGNANLTARKHVSLKGGLHKTHNK 429

Query: 421 IKVSEREQVQSEEVEEKTFRSEEVQGESLQSE----------EVQEKTLYVIKIENEEIP 480
            K+S+ E+VQSEEV +KTFRSEEV+GE+ QSE          EVQEKTLYVIK+ENEE+P
Sbjct: 430 NKLSDHERVQSEEVNKKTFRSEEVRGETFQSEEVQGETFQSVEVQEKTLYVIKVENEEMP 489

Query: 481 HQSD-QNETDDNMEPLPSSPPKSLSPPTSPYISANVEDQGVSEYTENTESEAQDDSYSES 540
           HQSD QNET DNMEPLPSS   SLSPP++ YI ANV+DQ VSEY   TESE ++DS+SES
Sbjct: 490 HQSDGQNETFDNMEPLPSSRLNSLSPPSAQYILANVKDQDVSEY---TESETENDSFSES 549

Query: 541 DEIGSMEADNVSN-EGGEKGRFQNRGMLQTKEKDPQSTKLSFRRGKIIDIPSESNSPRRL 600
           +E GSME DN S+ EGG+  R+QN  +L  KE+DPQSTKLSFRRGKIIDIP++SNSPRRL
Sbjct: 550 NEYGSMETDNASSEEGGQNARYQNPRILHNKEEDPQSTKLSFRRGKIIDIPAKSNSPRRL 609

Query: 601 KFRHGRLLGESQRAAVGLRKNFKRGTEVDSDTATAQETVVLRHQDV-QGKKDAQGLFNNV 660
           KFR GRLLGESQR A GLRKNFK GTEVD DTATAQE VVLRHQDV Q +KDAQGLFNNV
Sbjct: 610 KFRRGRLLGESQRDADGLRKNFKMGTEVDGDTATAQEIVVLRHQDVQQRRKDAQGLFNNV 669

BLAST of Tan0005347 vs. ExPASy TrEMBL
Match: A0A6J1JTD5 (uncharacterized protein LOC111487671 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111487671 PE=4 SV=1)

HSP 1 Score: 800.0 bits (2065), Expect = 7.2e-228
Identity = 480/697 (68.87%), Postives = 531/697 (76.18%), Query Frame = 0

Query: 1   MTVTVGTSSRRRSMGWMSSSNSGEEFVPHYLRASTGSCHDVCKYGKNHTFETKSRQPMLK 60
           MT T GTSSRR S GW+SSSNSGE+FVPHY R STGSCHD+CKYGKNHTFETKSRQP+  
Sbjct: 10  MTTTAGTSSRRSSTGWISSSNSGEKFVPHYRRISTGSCHDICKYGKNHTFETKSRQPV-- 69

Query: 61  ILTRRSLDGGSSVDSLVLPERKKTGGSLADCVIIPERKITTTTHMSKNVTRKSLDGGSSV 120
            +TR+SLDGG SVD +VL ERKKTGG   D V+IPERK T  T  SK++TRKSLD GSSV
Sbjct: 70  AMTRKSLDGGRSVDDVVLRERKKTGGGSVDSVVIPERKKTIMTERSKHMTRKSLDSGSSV 129

Query: 121 DSMVLPEREKTSSTRMLKNVARKSLDGGSSVDSVVLPERKKTTLTQMPKNVARKSFGG-- 180
           DSMVLPER+KT+STRM KNVAR SLDGGSSVD VVL ERKKT   +M KNVAR S  G  
Sbjct: 130 DSMVLPERKKTTSTRMSKNVARISLDGGSSVDGVVLLERKKTASPRMSKNVARSSIDGVV 189

Query: 181 -----------------------GSSVDSVILPERKKTTLTRAKAEFSSTSRISEADLTT 240
                                  GSSV+  ILPERKKT  TR KAE SSTSR+S A++TT
Sbjct: 190 LLERKKTISTRMSKNVGRISLDSGSSVNRTILPERKKTASTRTKAELSSTSRMSGANVTT 249

Query: 241 FIRPKLSIESSGFPRPVQREVLNERKKKLLPSPMQRAVLNERKKKLLAEPRTLSKPRSHT 300
             +                           PSP+QR VLNERK KLLAEP+ L+K RSHT
Sbjct: 250 LTK---------------------------PSPVQREVLNERKNKLLAEPKALAKSRSHT 309

Query: 301 ANALKNLKPETSSATRNPEDSVVQVVAKAKERKLPEKSDQISKLKSIKVKPLRSAVSSDN 360
           AN LKN KPETS+ATRNPEDS   V+AK KERKLPEKS+   KLKSIKV PLRSAVS+D 
Sbjct: 310 ANMLKNSKPETSTATRNPEDSA--VLAKTKERKLPEKSENFVKLKSIKVNPLRSAVSTDG 369

Query: 361 LRRKNDSKTGKSFGTSKVAAKKVVATSTGSFSSNSIYGTANLMARKRVNLKGVPLKTHNK 420
            RR N SK  K+FGTSKVAAKKVVATSTGSFSSNSIYG ANL ARK V+LKG   KTHNK
Sbjct: 370 SRRTNGSKMVKNFGTSKVAAKKVVATSTGSFSSNSIYGNANLTARKHVSLKGGLHKTHNK 429

Query: 421 IKVSEREQVQSEEVEEKTFRSEEVQGESLQSE----------EVQEKTLYVIKIENEEIP 480
            K+S+ E+VQSEEV +KTFRSEEV+GE+ QSE          EVQEKTLYVIK+ENEE+P
Sbjct: 430 NKLSDHERVQSEEVNKKTFRSEEVRGETFQSEEVQGETFQSVEVQEKTLYVIKVENEEMP 489

Query: 481 HQSD-QNETDDNMEPLPSSPPKSLSPPTSPYISANVEDQGVSEYTENTESEAQDDSYSES 540
           HQSD QNET DNMEPLPSS   SLSPP++ YI ANV+DQ VSEY   TESE ++DS+SES
Sbjct: 490 HQSDGQNETFDNMEPLPSSRLNSLSPPSAQYILANVKDQDVSEY---TESETENDSFSES 549

Query: 541 DEIGSMEADNVSN-EGGEKGRFQNRGMLQTKEKDPQSTKLSFRRGKIIDIPSESNSPRRL 600
           +E GSME DN S+ EGG+  R+QN  +L  KE+DPQSTKLSFRRGKIIDIP++SNSPRRL
Sbjct: 550 NEYGSMETDNASSEEGGQNARYQNPRILHNKEEDPQSTKLSFRRGKIIDIPAKSNSPRRL 609

Query: 601 KFRHGRLLGESQRAAVGLRKNFKRGTEVDSDTATAQETVVLRHQDV-QGKKDAQGLFNNV 660
           KFR GRLLGESQR A GLRKNFK GTEVD DTATAQE VVLRHQDV Q +KDAQGLFNNV
Sbjct: 610 KFRRGRLLGESQRDADGLRKNFKMGTEVDGDTATAQEIVVLRHQDVQQRRKDAQGLFNNV 669

BLAST of Tan0005347 vs. ExPASy TrEMBL
Match: A0A6J1GZM4 (uncharacterized protein LOC111459082 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111459082 PE=4 SV=1)

HSP 1 Score: 791.6 bits (2043), Expect = 2.6e-225
Identity = 483/744 (64.92%), Postives = 535/744 (71.91%), Query Frame = 0

Query: 1   MTVTVGTSSRRRSMGWMSSSNSGEEFVPHYLRASTGSCHDVCKYGKNHTFETKSRQPMLK 60
           MT T GT SRR S GW+SSSNSGE+FVPHY R STGSCHD CKYGKNHTFETKSRQPM  
Sbjct: 10  MTTTAGTGSRRSSTGWISSSNSGEKFVPHYRRISTGSCHDFCKYGKNHTFETKSRQPM-- 69

Query: 61  ILTRRSLDGGSSVDSLVLPERKKTGGSLADCVIIPERKITTTTHMSKNVTRKSLDGGSSV 120
            +TR+SLDGG SVDS+VL ERKKTGG   D V+IPERK T  T  SK++TRKSLD GSSV
Sbjct: 70  TMTRKSLDGGRSVDSVVLRERKKTGGGSGDSVVIPERKKTIMTERSKHMTRKSLDDGSSV 129

Query: 121 DSMVLPEREKTSSTRML----------------------KNVARKSLDGGSSVDSVVLPE 180
           DSMVLPER+KT+S RML                      KNVARKSLDGGSSVD VVL E
Sbjct: 130 DSMVLPERKKTTSPRMLKNVARISLDGESSVKTTSPRMSKNVARKSLDGGSSVDGVVLLE 189

Query: 181 RKKTTLTQMPKNVARKSFGGGSSVDSV--------------------------------- 240
           RKKTT T+M KNVAR S  GGSSVD V                                 
Sbjct: 190 RKKTTSTRMSKNVARISLDGGSSVDGVVLLERKKTASPRMSKNVGRSSIDGVVLLERKKT 249

Query: 241 -----------------------ILPERKKTTLTRAKAEFSSTSRISEADLTTFIRPKLS 300
                                  ILPERKKT  TR KAE SSTSR+S A++TT  R    
Sbjct: 250 ISTRMSKNVARISLDSGSSVNRTILPERKKTASTRTKAELSSTSRMSGANVTTLTR---- 309

Query: 301 IESSGFPRPVQREVLNERKKKLLPSPMQRAVLNERKKKLLAEPRTLSKPRSHTANALKNL 360
                                  PSP+QR VLNERKKKLLAEP+ L+K RSHTAN LKN 
Sbjct: 310 -----------------------PSPVQREVLNERKKKLLAEPKALAKSRSHTANTLKNS 369

Query: 361 KPETSSATRNPEDSVVQVVAKAKERKLPEKSDQISKLKSIKVKPLRSAVSSDNLRRKNDS 420
           KPETS+ATRNPEDS   V+AK KERKLPEK++   KLKSIKV PLRSAVS+D  RR N S
Sbjct: 370 KPETSTATRNPEDSA--VLAKTKERKLPEKTENFVKLKSIKVNPLRSAVSTDGSRRTNGS 429

Query: 421 KTGKSFGTSKVAAKKVVATSTGSFSSNSIYGTANLMARKRVNLKGVPLKTHNKIKVSERE 480
           K  K+ GTSKVAAK+VVATS+GSFSSNSIYG ANL ARKRV+LKG   KTHNK K+S+ +
Sbjct: 430 KMVKNLGTSKVAAKRVVATSSGSFSSNSIYGNANLSARKRVSLKGGLHKTHNKNKLSDHQ 489

Query: 481 QVQSEEVEEKTFRSEEVQGESLQSE-----EVQEKTLYVIKIENEEIPHQSD-QNETDDN 540
           QVQSEEV++KTFRSEEV+GE+ Q E     EVQEKTLYVIK+ENEE+PHQ+D QNET DN
Sbjct: 490 QVQSEEVDKKTFRSEEVRGETFQGETFRSVEVQEKTLYVIKVENEEMPHQTDGQNETFDN 549

Query: 541 MEPLPSSPPKSLSPPTSPYISANVEDQGVSEYTENTESEAQDDSYSESDEIGSMEADNVS 600
           +EPLPSSPP SLSPP++ YI A V+DQ VSEY   TESE ++DS+SES+E GSME DN S
Sbjct: 550 VEPLPSSPPNSLSPPSAQYILAKVKDQDVSEY---TESETENDSFSESNEYGSMETDNAS 609

Query: 601 N-EGGEKGRFQNRGMLQTKEKDPQSTKLSFRRGKIIDIPSESNSPRRLKFRHGRLLGESQ 660
           + EGG+  R+QN  ML  KE+DPQSTKLSFRRGKIIDIPS+SNSPRRLKFR GRLLGES+
Sbjct: 610 SEEGGQNARYQNPRMLHNKEEDPQSTKLSFRRGKIIDIPSKSNSPRRLKFRRGRLLGESR 669

BLAST of Tan0005347 vs. ExPASy TrEMBL
Match: A0A6J1H132 (uncharacterized protein LOC111459082 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111459082 PE=4 SV=1)

HSP 1 Score: 779.6 bits (2012), Expect = 1.0e-221
Identity = 477/739 (64.55%), Postives = 528/739 (71.45%), Query Frame = 0

Query: 1   MTVTVGTSSRRRSMGWMSSSNSGEEFVPHYLRASTGSCHDVCKYGKNHTFETKSRQPMLK 60
           MT T GT SRR S GW+SSSNSGE+FVPHY R STGSCHD CKYGKNHTFETKSRQPM  
Sbjct: 10  MTTTAGTGSRRSSTGWISSSNSGEKFVPHYRRISTGSCHDFCKYGKNHTFETKSRQPM-- 69

Query: 61  ILTRRSLDGGSSVDSLVLPERKKTGGSLADCVIIPERKITTTTHMSKNVTRKSLDGGSSV 120
            +TR+SLDGG SVDS+VL ERKKTGG   D V+IPERK T  T  SK++TRKSLD GSSV
Sbjct: 70  TMTRKSLDGGRSVDSVVLRERKKTGGGSGDSVVIPERKKTIMTERSKHMTRKSLDDGSSV 129

Query: 121 DSMVLPEREKTSSTRML----------------------KNVARKSLDGGSSVDSVVLPE 180
           DSMVLPER+KT+S RML                      KNVARKSLDGGSSVD VVL E
Sbjct: 130 DSMVLPERKKTTSPRMLKNVARISLDGESSVKTTSPRMSKNVARKSLDGGSSVDGVVLLE 189

Query: 181 RKKTTLTQMPKNVARKSFGGGSSVDSV--------------------------------- 240
           RKKTT T+M KNVAR S  GGSSVD V                                 
Sbjct: 190 RKKTTSTRMSKNVARISLDGGSSVDGVVLLERKKTASPRMSKNVGRSSIDGVVLLERKKT 249

Query: 241 -----------------------ILPERKKTTLTRAKAEFSSTSRISEADLTTFIRPKLS 300
                                  ILPERKKT  TR KAE SSTSR+S A++TT  R    
Sbjct: 250 ISTRMSKNVARISLDSGSSVNRTILPERKKTASTRTKAELSSTSRMSGANVTTLTR---- 309

Query: 301 IESSGFPRPVQREVLNERKKKLLPSPMQRAVLNERKKKLLAEPRTLSKPRSHTANALKNL 360
                                  PSP+QR VLNERKKKLLAEP+ L+K RSHTAN LKN 
Sbjct: 310 -----------------------PSPVQREVLNERKKKLLAEPKALAKSRSHTANTLKNS 369

Query: 361 KPETSSATRNPEDSVVQVVAKAKERKLPEKSDQISKLKSIKVKPLRSAVSSDNLRRKNDS 420
           KPETS+ATRNPEDS   V+AK KERKLPEK++   KLKSIKV PLRSAVS+D  RR N S
Sbjct: 370 KPETSTATRNPEDSA--VLAKTKERKLPEKTENFVKLKSIKVNPLRSAVSTDGSRRTNGS 429

Query: 421 KTGKSFGTSKVAAKKVVATSTGSFSSNSIYGTANLMARKRVNLKGVPLKTHNKIKVSERE 480
           K  K+ GTSKVAAK+VVATS+GSFSSNSIYG ANL ARKRV+LKG   KTHNK K+S+ +
Sbjct: 430 KMVKNLGTSKVAAKRVVATSSGSFSSNSIYGNANLSARKRVSLKGGLHKTHNKNKLSDHQ 489

Query: 481 QVQSEEVEEKTFRSEEVQGESLQSEEVQEKTLYVIKIENEEIPHQSD-QNETDDNMEPLP 540
           QVQSEEV  +TF     QGE+ +S EVQEKTLYVIK+ENEE+PHQ+D QNET DN+EPLP
Sbjct: 490 QVQSEEVRGETF-----QGETFRSVEVQEKTLYVIKVENEEMPHQTDGQNETFDNVEPLP 549

Query: 541 SSPPKSLSPPTSPYISANVEDQGVSEYTENTESEAQDDSYSESDEIGSMEADNVSN-EGG 600
           SSPP SLSPP++ YI A V+DQ VSEY   TESE ++DS+SES+E GSME DN S+ EGG
Sbjct: 550 SSPPNSLSPPSAQYILAKVKDQDVSEY---TESETENDSFSESNEYGSMETDNASSEEGG 609

Query: 601 EKGRFQNRGMLQTKEKDPQSTKLSFRRGKIIDIPSESNSPRRLKFRHGRLLGESQRAAVG 660
           +  R+QN  ML  KE+DPQSTKLSFRRGKIIDIPS+SNSPRRLKFR GRLLGES+R A G
Sbjct: 610 QNARYQNPRMLHNKEEDPQSTKLSFRRGKIIDIPSKSNSPRRLKFRRGRLLGESRRDADG 669

BLAST of Tan0005347 vs. TAIR 10
Match: AT5G39380.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 137.1 bits (344), Expect = 5.1e-32
Identity = 197/646 (30.50%), Postives = 275/646 (42.57%), Query Frame = 0

Query: 13  SMGWMSSSNSGEEFVPHYLRASTGSCHDVCKYGKNHTFETKSRQPMLKILTRRSLDGGSS 72
           S G   +S   E+ +PHYLRASTGSCHD+CKYGK      K  +   K + ++SLD    
Sbjct: 28  STGKAMTSKPKEKKIPHYLRASTGSCHDLCKYGKRQIPVEKPWRSSTKKIFKKSLDDN-- 87

Query: 73  VDSLVLPERKKTGGSLADCVIIPERKITTTTHMSKNVTRKSLDGGSSVDSMVLPEREKTS 132
                L E  K G S                 M K V     + G+  DS  + +RE   
Sbjct: 88  -----LNETLKPGSS----------------KMKKKVREVEKNEGTD-DSFEVIKRE--- 147

Query: 133 STRMLKNVARKSLDGGSSVDSVVLPERKKTTLTQMPKNVARKSFGGGSSVDSVILPERKK 192
              ++K  A     G    + +++    +T + Q+                      +KK
Sbjct: 148 ---VVKYQASGVSSGMRKPEVLIISSCDETPVKQI----------------------KKK 207

Query: 193 TTLTRAKAEFSSTSRISEADLTTFIRPKLSIESSGFPRPVQREVLNERKKKLLPSPMQRA 252
           TTL+                                              KL PSP   +
Sbjct: 208 TTLS---------------------------------------------SKLKPSPDLGS 267

Query: 253 VLNERKKKLLAEPRTLSKPRSHTANALKNLKPETSSATRNPEDSVVQVVAKAKERKLPEK 312
             +E    L  +P+ L K  S    AL   KP+      N E  V   V K K  K  + 
Sbjct: 268 RSSENVDAL--KPKVLKKSYS----ALTTSKPKV-----NHEKVVASPVLKPKMGKRNDG 327

Query: 313 SDQISKLKSIKVKPLRSAVSSDNLRRKNDSKTGKSFGTSKVAAKKVVATSTGSFS---SN 372
            D+                         D K  K   +S+VA+KK   T   S S   S 
Sbjct: 328 KDE-------------------------DGKVKKGTVSSRVASKKAPVTPRASLSPRLSV 387

Query: 373 SIYGTANLMARKRVNLKGVPLKTHNKIKVSEREQVQSEEVEEKTFRSEEVQGESLQSEEV 432
            + G+++L  RK  +LK            S R+  +   V     R++E   + L    V
Sbjct: 388 RLAGSSSL--RKSQSLKAA--------SSSSRQNQKPRPVN----RTDEF-NKQLDDYPV 447

Query: 433 QEKTLYVIKIE-NEEIPHQSDQNETDDNMEPLPSSPPKSLSPPTSPYISANVEDQGVSEY 492
           +EKTL+V+++E    +  ++DQN+       LP  PP   +P          ++  VSE 
Sbjct: 448 EEKTLHVVEMETTNNVVSENDQNQQGFVEPFLPPLPPTQSTPKD--------DECTVSET 506

Query: 493 TENTESEAQDDSYSESDEIGSMEADNVSNEGGEKGRFQNRGMLQTKEKDPQSTKLSFRRG 552
            E   +   +++ SE +EIG      +SN G +K R   +   +    D  + KL FRRG
Sbjct: 508 EEYEYTSGSNEAESEEEEIG------LSN-GEKKPRAARK---EGDSADEAARKLRFRRG 506

Query: 553 KIIDIPSESNSPRRLKFRHGRLLGESQRAAVGLRKNFKRGTEVDSDTATAQ-ETVVLRHQ 612
            I+D  +     R+LKFR GR LGE +     +R++FK+  ++  +      E VVLRHQ
Sbjct: 568 TIVDPDTVGEKARKLKFRRGRGLGEDKAQDAQVRRSFKKREDIREEEVNEDGEKVVLRHQ 506

Query: 613 DVQGKKDAQGLFNNVIEETASKLVETRKSKVKALVGAFETVISLQD 654
           DVQ +KDAQGLFNNVIEETASKLVE RKSKVKALVGAFETVISLQ+
Sbjct: 628 DVQ-EKDAQGLFNNVIEETASKLVEARKSKVKALVGAFETVISLQE 506

BLAST of Tan0005347 vs. TAIR 10
Match: AT5G15430.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 104.8 bits (260), Expect = 2.8e-22
Identity = 120/371 (32.35%), Postives = 184/371 (49.60%), Query Frame = 0

Query: 288 SATRNPEDSVVQVVAKAKERKLPEKSDQISKLKSIKVKPLRSAVSSDNLRRKNDSKTGKS 347
           S  R  +      V++ + +++ E+  +++ LK   V     A +++   R++  K  K 
Sbjct: 147 STKRKKKKKKTVYVSRGRAKEIVEQKRRVTALKLKAV-----AQTAEIALRRSTVKRKKM 206

Query: 348 FGTSKVAAKKVVATSTGSFSSNSIYGTANLMARKRVNLKGVPLKTHNKIKVSEREQVQSE 407
            G SK A +K    +    S +S   +  L  +K  N   VPLK   K            
Sbjct: 207 NGGSKAAEQKKAVMALRRASMSSKGCSRCLKTKKESNSLSVPLKKTRK------------ 266

Query: 408 EVEEKTFRSEEVQGESLQSEEVQEKTLYVIKIENEEIPHQSDQNETDDNMEPLPSSPPKS 467
            V +K              + V+EKTLYVIK+E  +   +S+ N+    +   P   PK 
Sbjct: 267 HVGDKC------------KDLVEEKTLYVIKMETVDEIVESELNQR--CVMDSPIDDPK- 326

Query: 468 LSPPTSPYISANVEDQGVSEYTEN-TESEAQDDSYSESDEIGSMEADNVSNEGGEKGRFQ 527
                    S   +D+G    TE+  ES  +++   E DE  S+  D  +   G+   F 
Sbjct: 327 ---------SEKSQDKGECIETEHEDESSQEEEDEEEEDENVSVSEDKNTTREGKSKAFS 386

Query: 528 NRGMLQTKEKDPQSTKLSFRRGKIIDIPSESNSPRRLKFRHGRLL-GESQRAAVGLRKNF 587
               +        + KL  RRGKIID  SE NSPR+LKF+ G+++ G    +  G R+  
Sbjct: 387 AESAI-----TGNAMKLRIRRGKIIDFGSEGNSPRKLKFKRGKIISGADTTSKSGGRRRL 446

Query: 588 K-RGTEVDSDTATAQE-TVVLRHQDVQGKKDAQG-LFNNVIEETASKLVETRKSKVKALV 647
           K +GT + +D    ++  VVL+HQD + K++++  LFN VI+ETA+KLV+TRKSKVKALV
Sbjct: 447 KTKGTNLSNDKEQQRKPRVVLKHQDTEKKRESRVLLFNKVIKETANKLVQTRKSKVKALV 471

Query: 648 GAFETVISLQD 654
           GAFE+VISLQ+
Sbjct: 507 GAFESVISLQE 471


HSP 2 Score: 40.4 bits (93), Expect = 6.4e-03
Identity = 96/440 (21.82%), Postives = 169/440 (38.41%), Query Frame = 0

Query: 5   VGTSSRRRSMGWMSSSNSGEEFVPHYLRASTGSCHDVCKYGKNHTFETKSRQPMLKILTR 64
           V  S RR S G +S   + E+ VP+YLR+ TGSCHD CKYG+    E K R P  K ++ 
Sbjct: 14  VNISWRRISTGKLSFLYTQEKVVPNYLRSPTGSCHDACKYGRKDESEDKPRVPHRKRVS- 73

Query: 65  RSLDGGSSVDSLVLPERKKTGGSLADCVIIPERKITTT---THMSKNV---------TRK 124
           RS  G  ++DS   P RKK   +L   ++ P R+  +     H    V          +K
Sbjct: 74  RSFSGAINLDS---PLRKK---ALTKPLLSPSRRCDSVGGFDHAKSQVRNFSSGVCDVKK 133

Query: 125 SLDGGSSVDSMVLPEREKTSSTRMLKNVARKSLDGGSSVDSVVLPERKKTTL-------- 184
           S   G++   + + E     ST+  K   +           +V  +R+ T L        
Sbjct: 134 SHADGTNEKVVSVSESRLADSTKRKKKKKKTVYVSRGRAKEIVEQKRRVTALKLKAVAQT 193

Query: 185 ---TQMPKNVARKSFGGGSSVDSVILPERKKTTLTRAKAEFSSTSRISEADLTTFIRPKL 244
                    V RK   GGS        E+KK  +   +A  SS         +  ++ K 
Sbjct: 194 AEIALRRSTVKRKKMNGGSKA-----AEQKKAVMALRRASMSSKG------CSRCLKTKK 253

Query: 245 SIESSGFPRPVQREVLNERKKKLLPSP----MQRAVLNERKKKLLAEPRTLSKPRSHTAN 304
              S   P    R+ + ++ K L+       ++   ++E  +  L +   +  P     +
Sbjct: 254 ESNSLSVPLKKTRKHVGDKCKDLVEEKTLYVIKMETVDEIVESELNQRCVMDSPIDDPKS 313

Query: 305 ALKNLKPETSSATRNPEDSVVQVVAKAKERKLPEKSDQ-ISKLKSIKVKPLRSAVSSDNL 364
                K E        E S  +   + ++  +    D+  ++    K     SA++ + +
Sbjct: 314 EKSQDKGECIETEHEDESSQEEEDEEEEDENVSVSEDKNTTREGKSKAFSAESAITGNAM 373

Query: 365 RRKNDSKTGKSFGTSKVAAKKVVATSTGSFSSNSIYGTANLMA----RKRVNLKGVPLKT 413
           + +        FG+   + +K+       F    I   A+  +    R+R+  KG  L  
Sbjct: 374 KLRIRRGKIIDFGSEGNSPRKL------KFKRGKIISGADTTSKSGGRRRLKTKGTNLSN 429

BLAST of Tan0005347 vs. TAIR 10
Match: AT5G61260.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 85.9 bits (211), Expect = 1.3e-16
Identity = 116/391 (29.67%), Postives = 184/391 (47.06%), Query Frame = 0

Query: 273 SHTANALKNLKPETSSATRNPEDSVV---QVVAKAKERKLPEKSDQISKLKSIKVKPL-- 332
           S T  +L ++  ++   T+ P+ SV      +A  K+      S+  SK  S   K +  
Sbjct: 134 SSTRKSLGSVSRQSPGITK-PDSSVSAKRDALAVKKKPCASVNSESSSKEGSEIAKSVDG 193

Query: 333 RSAVSSDNLRRKNDSKTGKSFGTSKVAAKKVVATSTGSFSSNSIYGTANLMARKRVNLKG 392
            S  S+D  R+  ++++G S        KKV A  T   S+++  G++ + A K  NLK 
Sbjct: 194 LSVKSNDRARKNKETESGLS---GSAVVKKVPALRTDKSSTSTGVGSSKVCAPK--NLKN 253

Query: 393 VPLKTHNKIKVSEREQVQSEEVEEKTFRSEEVQGESLQSEEVQEKTLYVIKIENEEIPHQ 452
           V        K    + +  E+V+EKT    E   + ++SE+        +K  N+ +   
Sbjct: 254 VE-------KAKTTQTISGEDVKEKTVCVVESSVKGVKSEKQPSSEKKTMKSGNKSL--- 313

Query: 453 SDQNETDDNMEPLPSSPPKSLSPPTSPYISANVEDQGVSEYTENTESEAQDDSYSESDEI 512
                         S+ PK  S PT   I   +                     ++  E 
Sbjct: 314 --------------STTPKRGSSPTK-QIPGKI-----------------STGLTKKKET 373

Query: 513 GSMEADNVSNEGGEKGRFQNRGMLQTKEKDPQSTKLSFRRGKIIDIPSESNSPRRLKFRH 572
           GS +    + +  +K R +  G+     K   + +++F++GK++D   E +SPR +KF+ 
Sbjct: 374 GSADVVEANPKPEKKVRPKKTGV-----KVSLAQQMTFKKGKVLDPKPEDSSPRWIKFKK 433

Query: 573 GRLLGESQRAAVGLRKNFKR-----GTEVDSDTATAQETVVLRHQDVQGKKDAQGLFNNV 632
            R++ E +  + G +KN K       T+ DS   + +E VVLRH+ V+GKK    LFNNV
Sbjct: 434 -RVVQELKTQSEGKKKNLKDRRLGVETKTDSCEGSKREKVVLRHRKVEGKKKMITLFNNV 470

Query: 633 IEETASKLVETRKSKVKALVGAFETVISLQD 654
           IEET +KL + RK KVKAL+GAFETVISLQD
Sbjct: 494 IEETVNKLTKVRKHKVKALIGAFETVISLQD 470

BLAST of Tan0005347 vs. TAIR 10
Match: AT5G07820.1 (Plant calmodulin-binding protein-related )

HSP 1 Score: 68.9 bits (167), Expect = 1.7e-11
Identity = 113/393 (28.75%), Postives = 186/393 (47.33%), Query Frame = 0

Query: 280 KNLKPETSSATRNPEDS--VVQVVAKAKERKLPEKSDQISKLKSIKVKPLRSAVSSDNLR 339
           K+  P  S   R  + +  VV   ++  + K P K D    LK+ +   +   V  D++ 
Sbjct: 161 KSTSPSVSPVVRTVKKTNLVVNKASRISQNKSP-KEDLSKNLKNKEKGKIVEPVRCDDVL 220

Query: 340 RKNDSKTGKSFGTSKVAAKKVVATSTGSFSSNSIYGTANLMARKRVNLKGVPLKTHNKIK 399
            K D +  K    S+++  K   +S      N      +   R    L+   L      +
Sbjct: 221 EKTDLEVKK---VSRISENK---SSKEDTLKNKEKAKIDEPVRCDDVLEKTSLDAQKVSR 280

Query: 400 VSEREQVQSEEVEEKTFRSEEVQGESLQSEEVQEKTLYVIKIENEEIPHQSDQ-----NE 459
           +SE +  + E ++    + +    E ++ ++  EKTLYV++   E+   +        +E
Sbjct: 281 ISENKNSKEERLKNLKNKEKTNIDEPVRPDDAVEKTLYVVESSVEKKKKKMSTKSVKISE 340

Query: 460 TDDNMEP--LPSSPPKSLS-----PPTSPYISANVEDQGVSEYTENTESEAQDDSYSESD 519
           T  + E   + S+  KSLS     PP S  ++ + + + + + T  +++     S  E  
Sbjct: 341 TQQSSEKKIIRSTGKKSLSLLPSLPPPSEVVTGS-DPRPIRQTTSRSKT-----SLPEKK 400

Query: 520 EIGSMEADNVSNEGGE-KGRFQNRGMLQTKEKDPQSTKLSFRRGKIIDIPSESNSPRRLK 579
           + GS  A+ V+N   E K R +  G+  T    P   +++F++GK+++   E ++   +K
Sbjct: 401 QSGS--ANLVTNPKPESKIRPKRIGLKVTPPPPPTKQQMNFKKGKVLEPKPEDSTTTSIK 460

Query: 580 FRHGRLLGESQRAA-VGLRKNF---KRGTEVDSDTATAQETVVLRHQDVQGKKDAQGLFN 639
           F+   +     R + V  +K     KR      +    +E VVLRH+ V+ KK  Q LFN
Sbjct: 461 FKKIVVQEPKLRTSDVNKKKKSLKDKREGVGKINGEGKREKVVLRHRKVEVKKKLQTLFN 520

Query: 640 NVIEETASKLVETRKSKVKALVGAFETVISLQD 654
           NVIEET +KL E RKSKVKALVGAFETVISLQD
Sbjct: 521 NVIEETVNKLEEVRKSKVKALVGAFETVISLQD 538

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
XP_022990927.11.1e-23571.32uncharacterized protein LOC111487671 isoform X2 [Cucurbita maxima][more]
XP_022990925.17.4e-23570.59uncharacterized protein LOC111487671 isoform X1 [Cucurbita maxima] >XP_022990926... [more]
XP_023541286.12.6e-23268.96uncharacterized protein LOC111801497 isoform X2 [Cucurbita pepo subsp. pepo] >XP... [more]
XP_023541278.12.6e-23268.96uncharacterized protein LOC111801497 isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022990929.11.5e-22768.87uncharacterized protein LOC111487671 isoform X3 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1JK935.5e-23671.32uncharacterized protein LOC111487671 isoform X2 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1JPA73.6e-23570.59uncharacterized protein LOC111487671 isoform X1 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1JTD57.2e-22868.87uncharacterized protein LOC111487671 isoform X3 OS=Cucurbita maxima OX=3661 GN=L... [more]
A0A6J1GZM42.6e-22564.92uncharacterized protein LOC111459082 isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1H1321.0e-22164.55uncharacterized protein LOC111459082 isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
Match NameE-valueIdentityDescription
AT5G39380.15.1e-3230.50Plant calmodulin-binding protein-related [more]
AT5G15430.12.8e-2232.35Plant calmodulin-binding protein-related [more]
AT5G61260.11.3e-1629.67Plant calmodulin-binding protein-related [more]
AT5G07820.11.7e-1128.75Plant calmodulin-binding protein-related [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012417Calmodulin-binding domain, plantSMARTSM01054CaM_binding_2coord: 539..650
e-value: 5.9E-36
score: 135.4
IPR012417Calmodulin-binding domain, plantPFAMPF07839CaM_bindingcoord: 542..650
e-value: 1.0E-35
score: 123.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 399..422
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 399..427
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 492..506
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 472..491
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 263..295
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 330..349
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 441..543
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..20
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 270..295
NoneNo IPR availablePANTHERPTHR33349:SF1EMB|CAB62594.1coord: 8..659
NoneNo IPR availablePANTHERPTHR33349EMB|CAB62594.1coord: 8..659

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0005347.1Tan0005347.1mRNA
Tan0005347.2Tan0005347.2mRNA
Tan0005347.3Tan0005347.3mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005516 calmodulin binding