Tan0005335 (gene) Snake gourd v1

Overview
NameTan0005335
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptioncytochrome P450 82A3-like
LocationLG01: 114357193 .. 114362392 (+)
RNA-Seq ExpressionTan0005335
SyntenyTan0005335
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATTTAATTGTCGTTGTTCTTGTCAAGTCACTATATCTATATGAGCTTAAAGTTAGTTAGTCCAACTAATGGTCATCCTAATCCTCTTCAGCAATCAAATTCCAATCTTGAATAATGGAGTTCCCACTTGCTTCCTTAAACCCCTTATCCGTTGCAACGCTCATTATTGTGCTCTTCGTTTCTTGTTATTTTTTGTGGAAGAAGATCGGGGGAGTTTGTGAGCCAAAGAAGGCCCCTTCGCCGCCGGGAGCGTGGCCTATAATTGGTCACCTCCATTTGCTCAACAAAGGGAAAATTCCCCACCATGTTCTTGGAGCCATGGCGGACAAATATGGGCCGATCTTCCGGTTGCAGCTCGGGTCTCGGTCCGCTCTGGTGGTGAGCAATTGGGAAATGGCCAAAGAAAGTTTGTGCACTAACGATAACGCCGCTGCGTCTCGCCCAGAACTCTCGGTCTCCAAGCATTTCTCTTACGACTTCGCCATGTTTGGCTTGGCTTCCTACAGCCCTTACTGGCGCGATATGCGTAAAATAGCCAACTTGGAACTCCTCTCTAACCCTCGTGTCGATCAGGTTCTACATAGTTACTCAACTCAAAAGTTTAAATTAAGTTATCTTAGGTCTTGAACATTGAACAATTCACCCCAAACACACTGTTTCGCTAACTCCAACACGGTTTCACTAATTTTCAAATAATGGTTTGCTTTTAGTTTATACAAACTTTTTTAAAAAACAACTATTTCGGTCTTTACTATATTATTTGTCCATCTAATTAACTACTTGTCTAATGCTCATCTCTAATTTATGTGGCTAGAAATTATGCTCTTGTTAGTATACATACTCAAATCTACGTGATTTTTCAAAAATGAAATATAAAGTAAAGACTAAAACAGTTATTTTTTTAAAGTTGAAGGACTAAAATAGAATTTAAAAAATTTTAAATACTAAAATGAAGCAAAACCAAAAGTTTAGGCCGCATTTGATAACTGTTTAATTTTTTATTTTTGGTATTTGAAAATTATGTTTTTTTTTTCCACAATTTCTCTTCATCATCTCTATTGATGATTTCCACCTTTCTCGAGGTAGTTTCTAGCCAAAATTCAAAATCAGAAATAAGTTTTTGAAAATTACTTTTTTTTAGTTCGTAAAATTTAGTTTGGTTTTTGAAGATATAGATACAAAAATAGATAAGAAAACATAGAAATTCATTAGCAATAAAAAAATGAAATGGTTATTAAACGAGATCTTGAACCAAAATTGAATAAATTGAAAGACGAAAATAGAATTTGAACCTAATTTTCAAGGGTTATGAGTTTGAAAATGAGCACAAAGTTGAAAATAAATGTAAAAGAAAAAACTTTTAAAAAAGGATTGATGGGAGAATATAACATTATAGAAATTCAAACATATTCTGGTGATGCTAGGTTGAAGCTTAAACTAGGTTACGGTATGTTTAATCTTGTTATTGATATTCTTTGAAACAGGTTAAGAACATCATGTTTGGTGAGATCGACACATCGTTGACAGAGCTGCACGAAAGATGGGCAGTCGAAAAGAACGAATCGGGGCAGGTTCCGGTGGAGATGAAGCGTTGGTTTGGGGATGCGATAGTGAATATGCTTCTCAAAACCATCATCGGAAAAAGATGTGTGGGTCCAAACGCCATGGGAGGTGAAAAAGAAGCAAAAGAGTTGCAGATGGCAATTAGAGACTCCTTTCATTTGATGGGTGAAGGGTTACTGAGAGATTACATTCCAGTGATTGGAAAGCTGGGATTTGACGGAAATGTTAAGGCAATGGAAAAAATAGCAAAACGAATCGACGCGATAATGGCAGGTTGGCTCGAAGAACACAAGCGAAACAGAGCTAATGGAAATTTGGAAAATAAAGAAAGAGATGTTAATTTTATGGATTCACTGCTTTCGTTATATGATGCCAAGAAGCTTCCGCGTTACTACGACGGCGATACCATTATCAAAGCCACAACCTTGGTACCATCTTCTTTTATCTATATTATTATCTTCTTTTAACTATATTATTATCATCTTTTAGGTTTAAGTTATCTTTTTTTTCTTTTCTTTTCTTTTTTTTTTTTTTTAATTTTTAGGTTAAAAAATCTTTGTTATTATTTAATGAGAAAAACTTGGGGGTAGAGGATGCACCCATCTCCAATGCAACCCACTTTGTAACCATATGTTTTTCTTTTCTTTTTTTTTATTAAAAAAACCAAAAGATTTTATGTGGTTGCAAATAGCGGTGTATACGGTTCGGTTCGATCTGGTTTTTATTCGGTCCTTAGAGTGGTTAAACCAAGCAAATCGAAAACATTCTTCAGCTTCGATTTAAAACTGAACCAGTTTACTTCGGTTTATTAGGTTTGGTTTCGGTTCTTAACCATTTAAAAAAATGCAATGAAAGTTTAAAAAGAAAATGACTGTCGGCAAGAATTGAGAAGGTTGTAGTATTTATGTCGGTGATAGAAAACAAAAAAGAGCGTGAGAAAGAGGAATGAGAACTTTTGTGCCACCGCCATGACTACCATTGATTCGTTGCTGTGGACTATAAGAGAAGAGGGTGAATGGAAGGAGAAAGAGGAATGTGGGAGACGGAAAGAAAGAAAGAAGAACAAAACGTTAAAGAAGAGAGGATAGGGGAAGAAGAAAGTGAGTTTGAGAGAAGAAGAAAGTGGGTTTGATAAAACTTAGAAGAGGACAAAAAGTAAGAGATAATGAAAAATAAAAGTGAGAGAAGATGAAAAGACGACCGGAGAAGATATAAAGAAGTCACGTGATTGTTGTAAAATGGAGAGAGAGAAAAGTTGCTGCAATTAATTAAAAAGTGGAGTTTTCTTGAAATTGTGTTAGTTAAATTAAGTGGGTTTTCTTGAATGTTAAGTTGGATTTTATAGAATAGACTTTAAAGGAGTTTTTATTTTTTTTTAATCACATTCAATCAGTTTTATTGATTTTGAAAAATTCTGAACGGTAAATCGAATCGAATATTACGGTTTTGTATAATTTTAAACCGAAAAACTATTTTTTTTTCAAAATAATCGAATCAAACCAGTCAGTTTGGATCGATTGGTTCGATTTTCTTAGTTTATTACACTCCTGATTGTAAAATGGATTGCATGGGAGATGGTGCCGCTCATGCTATACCCAAATTCTTTCCTTATTTAATTATCATCTAACAACCATCCACTTTCTTCAATCAATCTATGTGGATAATTTAATATATCTGCAAGAGGTGCTAGCAGTAATAAAGGTGTTGTTTAAACTTCATTCATTCTTAAAATTATGTTAAGTTGGAAGTTTAGAATGTCAGCCTTAAGGAAATATCAAAATCTCAATTTATACAAATATGAATGGAAATATTGATAAAATGTTGATATCGATAGATATTTCTAAAAAAATTTGAAAAAAAAAAATTAAAAAGATATTTAAATTAATAAATAACTATTTTATTCTTTATCAATGAGTTAAAATACTTATTATTAATATTATATTCATATTAGGTCATATTTTGTTGTTATTTTTTATAATATTTCATTAATTTTATATAGTATAATAAAAATGTTGATTCACCTCTTACATCTATATTTAACCTATAGATATGTAAAAATGATGAAAATATCACTGTCGGAAAAAATTTAATACTATCATTAAATTACAAATTTAATTGATGATGTTTATTTAGTCCTAAATTTGTAAACTTTTCTTTATTATATTCATGATTTTTTATAGAATATTATATTCATGAATTTTAAATTACAATTTTGTCTCTAGCTCAGCTAAACCACAATAGTATGTCTCCCCACTAATTTCAACCTAACAAACAAATTATATATACTTTCTCTAAACCAATCTTATCCATATCTTCTATCTTGACTAATTTAGAACAAATTATTGAATAAGGCCGCCAATCATAACGATTGGAAGATTTAACAATTTCTCTTAATTTATATATATATATAACACGTATATGTGCAGACCATGATTTCAGGTGGAACTGAAAGCAGCACCGTGACTCTAACATGGGCCATGTCGTTATTACTAAACAATCCTCACACCCTTAGAAGAGCATATCAAGAGCTCGACGAAGTTGTCGGTAGAGACAGACGAGTGAACGAGGCTGACATAACCAAGTTAGTTTATTTACAAGCCATAGTCAAAGAGACAATGCGATTGTCCCCAGCTGGGCCTCTATTGGGACCTCGTGAGTTCTACAAGGACTGTGTTGTTGCAGGCTATTTCATCCCCAAAGGCACTCAACTAATCCCAAACATTTGGAAGATCCAAACCGACCCACGGGTCTGGCCCGACCCGTTTGAGTTCAAACCGGAGCGCTTTTTGACAACCCATAAGGATGTGGATTTGAAAGGGAATAACTTCGAGTTGATTCCCTTTGGGAGTGGCCGAAGAGGTTGTCCTGCCATTGCGTTTGGGCTTCAAATGGTGCATTTCACTTTGGCTGGATTTTTACAGTCTTTTGATATCAAAAACCCGACAAGTGAACCAGTTGATATGACCGAGAATTTCGGAATGGCGAATGAGAAAGTGGTGCCTCTCGATGTTTTGGTCACCCCACGGTTACCTTTGCATCTTCACGGTATAGTATAGTTGTTTATGTTAAAGTATTAATATAAATAATTTATCACCATCGACTTAAACACTTAAGTTGATTGATAATTTATTAGTTTCAATGTTCATTTTGTGTGTTGATAGCAGATGTTAGTATTCGATGTCGGTTACATCTTCTATTGTATGTGCTAGTGTCGGCTACATAATAAGGGCACATGTTCCTTCAAAAAAAAAAAAAGGGAAGGATTCTATAAGAAATGGTTTGGTAATGTTAAGTGTTTTGGTAAGGACTATTTTGTTGTTTGATTTAAGTATTAGAAAAATATTTTTATCTAATTAAATGTGTTCTTTTAAATGATTAATATTGACTTTTATGGCATTGAAAAAAAAAACAGTAAAATGAAAATGATAAAAAAATATTTAAAATATCGGAATTGAAAACATTCCAATTAAACAAAACTAAAATTAAAAGACAAGAAGAAGAACGAGAGAGAAATGAGAGAATATATGTAATGTGGAGAGATATTATGATATGAACTACTAGGGAATCAAATTTGTTATTTTCTAATTTTTATATAATTCTATTATTTTTCAATTTTATCTTTTTTATGATATTTTAAG

mRNA sequence

ATTTAATTGTCGTTGTTCTTGTCAAGTCACTATATCTATATGAGCTTAAAGTTAGTTAGTCCAACTAATGGTCATCCTAATCCTCTTCAGCAATCAAATTCCAATCTTGAATAATGGAGTTCCCACTTGCTTCCTTAAACCCCTTATCCGTTGCAACGCTCATTATTGTGCTCTTCGTTTCTTGTTATTTTTTGTGGAAGAAGATCGGGGGAGTTTGTGAGCCAAAGAAGGCCCCTTCGCCGCCGGGAGCGTGGCCTATAATTGGTCACCTCCATTTGCTCAACAAAGGGAAAATTCCCCACCATGTTCTTGGAGCCATGGCGGACAAATATGGGCCGATCTTCCGGTTGCAGCTCGGGTCTCGGTCCGCTCTGGTGGTGAGCAATTGGGAAATGGCCAAAGAAAGTTTGTGCACTAACGATAACGCCGCTGCGTCTCGCCCAGAACTCTCGGTCTCCAAGCATTTCTCTTACGACTTCGCCATGTTTGGCTTGGCTTCCTACAGCCCTTACTGGCGCGATATGCGTAAAATAGCCAACTTGGAACTCCTCTCTAACCCTCGTGTCGATCAGGTTAAGAACATCATGTTTGGTGAGATCGACACATCGTTGACAGAGCTGCACGAAAGATGGGCAGTCGAAAAGAACGAATCGGGGCAGGTTCCGGTGGAGATGAAGCGTTGGTTTGGGGATGCGATAGTGAATATGCTTCTCAAAACCATCATCGGAAAAAGATGTGTGGGTCCAAACGCCATGGGAGGTGAAAAAGAAGCAAAAGAGTTGCAGATGGCAATTAGAGACTCCTTTCATTTGATGGGTGAAGGGTTACTGAGAGATTACATTCCAGTGATTGGAAAGCTGGGATTTGACGGAAATGTTAAGGCAATGGAAAAAATAGCAAAACGAATCGACGCGATAATGGCAGGTTGGCTCGAAGAACACAAGCGAAACAGAGCTAATGGAAATTTGGAAAATAAAGAAAGAGATGTTAATTTTATGGATTCACTGCTTTCGTTATATGATGCCAAGAAGCTTCCGCGTTACTACGACGGCGATACCATTATCAAAGCCACAACCTTGACCATGATTTCAGGTGGAACTGAAAGCAGCACCGTGACTCTAACATGGGCCATGTCGTTATTACTAAACAATCCTCACACCCTTAGAAGAGCATATCAAGAGCTCGACGAAGTTGTCGGTAGAGACAGACGAGTGAACGAGGCTGACATAACCAAGTTAGTTTATTTACAAGCCATAGTCAAAGAGACAATGCGATTGTCCCCAGCTGGGCCTCTATTGGGACCTCGTGAGTTCTACAAGGACTGTGTTGTTGCAGGCTATTTCATCCCCAAAGGCACTCAACTAATCCCAAACATTTGGAAGATCCAAACCGACCCACGGGTCTGGCCCGACCCGTTTGAGTTCAAACCGGAGCGCTTTTTGACAACCCATAAGGATGTGGATTTGAAAGGGAATAACTTCGAGTTGATTCCCTTTGGGAGTGGCCGAAGAGGTTGTCCTGCCATTGCGTTTGGGCTTCAAATGGTGCATTTCACTTTGGCTGGATTTTTACAGTCTTTTGATATCAAAAACCCGACAAGTGAACCAGTTGATATGACCGAGAATTTCGGAATGGCGAATGAGAAAGTGGTGCCTCTCGATGTTTTGGTCACCCCACGGTTACCTTTGCATCTTCACGGTATAGTATAGTTGTTTATGTTAAAGTATTAATATAAATAATTTATCACCATCGACTTAAACACTTAAGTTGATTGATAATTTATTAGTTTCAATGTTCATTTTGTGTGTTGATAGCAGATGTTAGTATTCGATGTCGGTTACATCTTCTATTGTATGTGCTAGTGTCGGCTACATAATAAGGGCACATGTTCCTTCAAAAAAAAAAAAAGGGAAGGATTCTATAAGAAATGGTTTGGTAATGTTAAGTGTTTTGGTAAGGACTATTTTGTTGTTTGATTTAAGTATTAGAAAAATATTTTTATCTAATTAAATGTGTTCTTTTAAATGATTAATATTGACTTTTATGGCATTGAAAAAAAAAACAGTAAAATGAAAATGATAAAAAAATATTTAAAATATCGGAATTGAAAACATTCCAATTAAACAAAACTAAAATTAAAAGACAAGAAGAAGAACGAGAGAGAAATGAGAGAATATATGTAATGTGGAGAGATATTATGATATGAACTACTAGGGAATCAAATTTGTTATTTTCTAATTTTTATATAATTCTATTATTTTTCAATTTTATCTTTTTTATGATATTTTAAG

Coding sequence (CDS)

ATGGAGTTCCCACTTGCTTCCTTAAACCCCTTATCCGTTGCAACGCTCATTATTGTGCTCTTCGTTTCTTGTTATTTTTTGTGGAAGAAGATCGGGGGAGTTTGTGAGCCAAAGAAGGCCCCTTCGCCGCCGGGAGCGTGGCCTATAATTGGTCACCTCCATTTGCTCAACAAAGGGAAAATTCCCCACCATGTTCTTGGAGCCATGGCGGACAAATATGGGCCGATCTTCCGGTTGCAGCTCGGGTCTCGGTCCGCTCTGGTGGTGAGCAATTGGGAAATGGCCAAAGAAAGTTTGTGCACTAACGATAACGCCGCTGCGTCTCGCCCAGAACTCTCGGTCTCCAAGCATTTCTCTTACGACTTCGCCATGTTTGGCTTGGCTTCCTACAGCCCTTACTGGCGCGATATGCGTAAAATAGCCAACTTGGAACTCCTCTCTAACCCTCGTGTCGATCAGGTTAAGAACATCATGTTTGGTGAGATCGACACATCGTTGACAGAGCTGCACGAAAGATGGGCAGTCGAAAAGAACGAATCGGGGCAGGTTCCGGTGGAGATGAAGCGTTGGTTTGGGGATGCGATAGTGAATATGCTTCTCAAAACCATCATCGGAAAAAGATGTGTGGGTCCAAACGCCATGGGAGGTGAAAAAGAAGCAAAAGAGTTGCAGATGGCAATTAGAGACTCCTTTCATTTGATGGGTGAAGGGTTACTGAGAGATTACATTCCAGTGATTGGAAAGCTGGGATTTGACGGAAATGTTAAGGCAATGGAAAAAATAGCAAAACGAATCGACGCGATAATGGCAGGTTGGCTCGAAGAACACAAGCGAAACAGAGCTAATGGAAATTTGGAAAATAAAGAAAGAGATGTTAATTTTATGGATTCACTGCTTTCGTTATATGATGCCAAGAAGCTTCCGCGTTACTACGACGGCGATACCATTATCAAAGCCACAACCTTGACCATGATTTCAGGTGGAACTGAAAGCAGCACCGTGACTCTAACATGGGCCATGTCGTTATTACTAAACAATCCTCACACCCTTAGAAGAGCATATCAAGAGCTCGACGAAGTTGTCGGTAGAGACAGACGAGTGAACGAGGCTGACATAACCAAGTTAGTTTATTTACAAGCCATAGTCAAAGAGACAATGCGATTGTCCCCAGCTGGGCCTCTATTGGGACCTCGTGAGTTCTACAAGGACTGTGTTGTTGCAGGCTATTTCATCCCCAAAGGCACTCAACTAATCCCAAACATTTGGAAGATCCAAACCGACCCACGGGTCTGGCCCGACCCGTTTGAGTTCAAACCGGAGCGCTTTTTGACAACCCATAAGGATGTGGATTTGAAAGGGAATAACTTCGAGTTGATTCCCTTTGGGAGTGGCCGAAGAGGTTGTCCTGCCATTGCGTTTGGGCTTCAAATGGTGCATTTCACTTTGGCTGGATTTTTACAGTCTTTTGATATCAAAAACCCGACAAGTGAACCAGTTGATATGACCGAGAATTTCGGAATGGCGAATGAGAAAGTGGTGCCTCTCGATGTTTTGGTCACCCCACGGTTACCTTTGCATCTTCACGGTATAGTATAG

Protein sequence

MEFPLASLNPLSVATLIIVLFVSCYFLWKKIGGVCEPKKAPSPPGAWPIIGHLHLLNKGKIPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKESLCTNDNAAASRPELSVSKHFSYDFAMFGLASYSPYWRDMRKIANLELLSNPRVDQVKNIMFGEIDTSLTELHERWAVEKNESGQVPVEMKRWFGDAIVNMLLKTIIGKRCVGPNAMGGEKEAKELQMAIRDSFHLMGEGLLRDYIPVIGKLGFDGNVKAMEKIAKRIDAIMAGWLEEHKRNRANGNLENKERDVNFMDSLLSLYDAKKLPRYYDGDTIIKATTLTMISGGTESSTVTLTWAMSLLLNNPHTLRRAYQELDEVVGRDRRVNEADITKLVYLQAIVKETMRLSPAGPLLGPREFYKDCVVAGYFIPKGTQLIPNIWKIQTDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPAIAFGLQMVHFTLAGFLQSFDIKNPTSEPVDMTENFGMANEKVVPLDVLVTPRLPLHLHGIV
Homology
BLAST of Tan0005335 vs. ExPASy Swiss-Prot
Match: O49858 (Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 6.8e-149
Identity = 261/517 (50.48%), Postives = 357/517 (69.05%), Query Frame = 0

Query: 7   SLNPLSVATLIIVLFVSCYFLWKKIGGVCEPKKAPSPPGAWPIIGHLHLLNKGKIPHHVL 66
           +LN +++A+++ ++F+ C FL++K       K AP   GAWPI+GHL LLN  + PH VL
Sbjct: 9   NLNTIAIASILSLIFL-CLFLYRKNS---RGKDAPVVSGAWPILGHLSLLNGSQTPHKVL 68

Query: 67  GAMADKYGPIFRLQLGSRSALVVSNWEMAKESLCTNDNAAASRPELSVSKHFSYDFAMFG 126
           GA+ADKYGP+F ++LG + ALV+SNWEM+KE   TND A +SRP+L   +  SY+ A  G
Sbjct: 69  GALADKYGPLFTIKLGMKPALVLSNWEMSKELFTTNDLAVSSRPKLVAVEVMSYNQAFVG 128

Query: 127 LASYSPYWRDMRKIANLELLSNPRVDQVKNIMFGEIDTSLTELHERWA-VEKNESGQVPV 186
           LA Y PYWR++RKI   E LSN R++Q  +I   E+ TS+ EL + W+   KNES    V
Sbjct: 129 LAPYGPYWRELRKIVTFEFLSNRRIEQRNHIRVSEVRTSIKELFDIWSNGNKNESRYTLV 188

Query: 187 EMKRWFGDAIVNMLLKTIIGKRCVGPNAMGGEKEAKELQMAIRDSFHLMGEGLLRDYIPV 246
           ++K+W      NM+++ ++GKR  G   + G+ +A+     IR+  +LMG   + D +P 
Sbjct: 189 DIKQWLAYLTFNMVVRMVVGKRYFGVMHVEGKDKAQRFMKNIREFMNLMGTFTVADGVPC 248

Query: 247 IGKLGFDGNVKAMEKIAKRIDAIMAGWLEEHKRNRANGNLENKERDVNFMDSLLSLYDAK 306
           +  L   G+ KAM+  AK +D +++ WLEEH++ +  G  EN E D +FMD ++S  +  
Sbjct: 249 LRWLDLGGHEKAMKANAKEVDKLLSEWLEEHRQKKLLG--ENVESDRDFMDVMISALNGA 308

Query: 307 KLPRYYDGDTIIKATTLTMISGGTESSTVTLTWAMSLLLNNPHTLRRAYQELDEVVGRDR 366
           ++   +D DTI KAT+L +I GGT+S+ VTLTWA+SLLL NP  L +A +E+D  +G+D 
Sbjct: 309 QIGA-FDADTICKATSLELILGGTDSTAVTLTWALSLLLRNPLALGKAKEEIDMQIGKDE 368

Query: 367 RVNEADITKLVYLQAIVKETMRLSPAGPLLGPREFYKDCVVAGYFIPKGTQLIPNIWKIQ 426
            + E+DI+KLVYLQAIVKET+RL P  P   PREF ++C++ GY I KGT+LI N+WKI 
Sbjct: 369 YIRESDISKLVYLQAIVKETLRLYPPAPFSSPREFTENCILGGYHIKKGTRLIHNLWKIH 428

Query: 427 TDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPAIAFGLQMVHFTLAGF 486
            DP VW DP EFKPERFLTTHKDVDL+G+NFEL+PFGSGRR C  ++ GL MVHFTLA  
Sbjct: 429 RDPSVWSDPLEFKPERFLTTHKDVDLRGHNFELLPFGSGRRVCAGMSLGLNMVHFTLANL 488

Query: 487 LQSFDIKNPTSEPVDMTENFGMANEKVVPLDVLVTPR 523
           L SFDI NP++EPVDMTE FG  N K  PL++LV PR
Sbjct: 489 LHSFDILNPSAEPVDMTEFFGFTNTKATPLEILVKPR 518

BLAST of Tan0005335 vs. ExPASy Swiss-Prot
Match: O49859 (Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1)

HSP 1 Score: 511.9 bits (1317), Expect = 8.6e-144
Identity = 259/526 (49.24%), Postives = 361/526 (68.63%), Query Frame = 0

Query: 1   MEFPLASLNPLSVATLIIVLFVSCYFLW---KKIGGVCEPKKAPSPPGAWPIIGHLHLLN 60
           ME  L  LN  ++  ++ ++F+ C FL+   KK+ G    K+AP+  GAWPI GHL LL 
Sbjct: 1   MELVLHFLNTTTIG-VVSLIFLLCLFLYGPLKKVHG--SSKEAPTVGGAWPIFGHLPLLI 60

Query: 61  KGKIPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKESLCTNDNAAASRPELSVSKH 120
             K PH  LGA+A+K+GP+F ++LG++ ALVVS+WEMA+E   TND A ++RP+L V++ 
Sbjct: 61  GSKSPHKALGALAEKHGPLFTIKLGAKKALVVSDWEMARECFTTNDVAVSARPKLLVAEL 120

Query: 121 FSYDFAMFGLASYSPYWRDMRKIANLELLSNPRVDQVKNIMFGEIDTSLTELHERWAVEK 180
             Y+ AM  +A Y PYWR++RKI   E+LS+ RV+Q++++   E+  S+ EL++ W  +K
Sbjct: 121 MCYNNAMLLVAPYGPYWRELRKIIVTEILSSSRVEQLQDVRVSEVQNSIVELYDVWRSQK 180

Query: 181 NESGQVPVEMKRWFGDAIVNMLLKTIIGKRCVGPNAMGGEKEAKELQMAIRDSFHLMGEG 240
           NES    VE+K+WF   I NM+L+ ++GKR +  +A   +++A++   A+ +   L G  
Sbjct: 181 NESDYASVELKQWFAQPIFNMVLRMVVGKRFL--SATATDEKAEKCVKAVDEFMRLAGVF 240

Query: 241 LLRDYIPVIGKLGFDGNVKAMEKIAKRIDAIMAGWLEEHKRNRANGNLENKERDVNFMDS 300
            + D IP +  L F G  KAM++ AK +D +++ WLEEH++ RA G  E  +   +FM+ 
Sbjct: 241 TVGDAIPYLRWLDFGGYEKAMKETAKELDVMISEWLEEHRQKRALG--EGVDGAQDFMNV 300

Query: 301 LLSLYDAKKLPRYYDGDTIIKATTLTMISGGTESSTVTLTWAMSLLLNNPHTLRRAYQEL 360
           +LS  D K +    D DT+IK+T LT+I  GTE+S  T+ WAM L+L NP  L     EL
Sbjct: 301 MLSSLDGKTIDG-IDADTLIKSTVLTIIQAGTEASISTIIWAMCLILKNPLILENK-AEL 360

Query: 361 DEVVGRDRRVNEADITKLVYLQAIVKETMRLSPAGPLLGPREFYKDCVVAGYFIPKGTQL 420
           D  VG+DR + E+DI+ LVYLQA+VKET+RL   GPL  PREF +DC + GY + KGT+L
Sbjct: 361 DIQVGKDRCICESDISNLVYLQAVVKETLRLYAPGPLSSPREFAEDCTLGGYHVKKGTRL 420

Query: 421 IPNIWKIQTDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPAIAFGLQM 480
           I NIWKI TDP VW DPFEFKP+RFLTTHKD+D+KG++F+L+PFGSGRR CP I+FGLQ 
Sbjct: 421 ITNIWKIHTDPNVWSDPFEFKPDRFLTTHKDIDVKGHHFQLLPFGSGRRVCPGISFGLQT 480

Query: 481 VHFTLAGFLQSFDIKNPTSEPVDMTENFGMANEKVVPLDVLVTPRL 524
           VH  LA FL SF+I NP++EP+DMTE FG+ N K  PL+VLV P L
Sbjct: 481 VHLALASFLHSFEILNPSTEPLDMTEAFGVTNTKATPLEVLVKPCL 517

BLAST of Tan0005335 vs. ExPASy Swiss-Prot
Match: Q43068 (Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2)

HSP 1 Score: 488.0 bits (1255), Expect = 1.3e-136
Identity = 240/535 (44.86%), Postives = 335/535 (62.62%), Query Frame = 0

Query: 8   LNPLSVATLIIVLFVSCYFLWKKIGGVCEPKKAPSPPGAWPIIGHLHLLNKGKIPHHVLG 67
           LN L+  T+  +  +S  F   +   V   K+ P   G+WP++GHL L+   + PH  LG
Sbjct: 3   LNYLNTTTIAFISLISLLFFLFRFSKVSHTKEPPIISGSWPLLGHLPLMRNTQTPHKTLG 62

Query: 68  AMADKYGPIFRLQLGSRSALVVSNWEMAKESLCTNDNAAASRPELSVSKHFSYDFAMFGL 127
           A+ DKYGPIF ++LG+ +ALV+SNWE+AKE    ND   +SRP+    +  SY+ A  G 
Sbjct: 63  ALVDKYGPIFTIKLGATNALVLSNWELAKECFTKNDIVVSSRPKPVAVELMSYNQAFIGW 122

Query: 128 ASYSPYWRDMRKIANLELLSNPRVDQVKNIMFGEIDTSLTELHERWA------------- 187
           A Y  YWR +RKI  LE+LSN R++ + +I   E+ TS+ EL   W+             
Sbjct: 123 APYGAYWRQLRKIVTLEILSNRRIELLSHIRVSEVQTSIKELVNVWSNQISSQYGLLDDT 182

Query: 188 ------VEKNESGQVPVEMKRWFGDAIVNMLLKTIIGKRCVGPNAMGGEKEAKELQMAIR 247
                  E + +  V VE+K+WF    +NM+L+ ++GKRC G   +  ++EAK     IR
Sbjct: 183 KSSSTNDEPSTTDYVSVELKKWFAQLTLNMVLRMVVGKRCFGDVDVENKEEAKRFLENIR 242

Query: 248 DSFHLMGEGLLRDYIPVIGKLGFDGNVKAMEKIAKRIDAIMAGWLEEHKRNRANGNLENK 307
           D   L+G   + D +P +  L   G+ K M+K AK+ D ++  WLEEH+  +  G+ +  
Sbjct: 243 DFMRLIGTFTVGDGVPFLKWLDLGGHEKEMKKCAKKFDVMLNEWLEEHREKKGLGSEDKV 302

Query: 308 ERDVNFMDSLLSLYDAKKLPRYYDGDTIIKATTLTMISGGTESSTVTLTWAMSLLLNNPH 367
             + +FMD++L +   K +   +D DTIIKATTL +I GG++++  TLTWAM LLL +PH
Sbjct: 303 VGERDFMDAMLLVLKDKPI-EGFDVDTIIKATTLELILGGSDTTAGTLTWAMCLLLKHPH 362

Query: 368 TLRRAYQELDEVVGRDRRVNEADITKLVYLQAIVKETMRLSPAGPLLGPREFYKDCVVAG 427
            L +  +EL+  +G++R VNE+DI KLVYL AI+KET+RL P  P   PREF +DC + G
Sbjct: 363 VLEKLKEELNTYIGKERCVNESDINKLVYLHAIIKETLRLYPPAPFSSPREFTEDCTIGG 422

Query: 428 YFIPKGTQLIPNIWKIQTDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGC 487
           Y I KGT+L+PN+WKI  DP VWPDP EFKPERFL+THKDVD++G NFEL+PFGSGRR C
Sbjct: 423 YHIKKGTRLMPNLWKIHRDPSVWPDPLEFKPERFLSTHKDVDVRGQNFELLPFGSGRRMC 482

Query: 488 PAIAFGLQMVHFTLAGFLQSFDIKNPTSEPVDMTENFGMANEKVVPLDVLVTPRL 524
             ++ GL MVH+ LA FL SF+I NP+ E +D+TE       K  PL+VLV P L
Sbjct: 483 AGMSLGLHMVHYILANFLHSFEILNPSPESIDVTEVLEFVTTKATPLEVLVKPCL 536

BLAST of Tan0005335 vs. ExPASy Swiss-Prot
Match: A0A0N7F297 (Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP82D61 PE=1 SV=1)

HSP 1 Score: 486.5 bits (1251), Expect = 3.8e-136
Identity = 247/521 (47.41%), Postives = 332/521 (63.72%), Query Frame = 0

Query: 7   SLNPLSVATLIIVLFVSC--YFLWKKIGGVCEPKKAPSPPGAWPIIGHLHLLNKGKIPHH 66
           SL+ L    L   + + C  YF+WKK     + K+ P P GAWPIIGHLHLL +G +PH 
Sbjct: 3   SLHCLETLLLGFFVLLPCFFYFVWKKPNN--KIKEPPQPAGAWPIIGHLHLLARGDLPHK 62

Query: 67  VLGAMADKYGPIFRLQLGSRSALVVSNWEMAKESLCTNDNAAASRPELSVSKHFSYDFAM 126
           +L + ADK GP+F++QLG   ALVV+N E+AKE   TND    +RP    +K   Y++ M
Sbjct: 63  ILSSFADKNGPVFKIQLGVHQALVVNNSEIAKECFTTNDRFFLNRPSGVAAKIMGYNYVM 122

Query: 127 FGLASYSPYWRDMRKIANLELLSNPRVDQVKNIMFGEIDTSLTELHERWAVEKNESGQVP 186
            G+A Y PYWRDMRKI  LE LSN R+  +K++   EI  S  EL++ W  E        
Sbjct: 123 LGVAPYGPYWRDMRKIIMLEFLSNRRLQSLKHVWHSEISISSKELYKLW--ETQNIDFCL 182

Query: 187 VEMKRWFGDAIVNMLLKTIIGKRCVGPNAMGG--EKEAKELQMAIRDSFHLMGEGLLRDY 246
           V+MK+W  D  +NM +K ++GKR  G  +     E E+      +R+ F LMG  +L DY
Sbjct: 183 VDMKQWLADLTLNMSVKMVVGKRFFGSASASACEETESSNCPKTLRNMFRLMGSFVLSDY 242

Query: 247 IPVIGKLGFDGNVKAMEKIAKRIDAIMAGWLEEHKRNRANGNLENKERDVNFMDSLLSLY 306
           +P +  L   G+ K M++  K +D +  GWL+EHKR R +G  E+ ++D  FMD +LS+ 
Sbjct: 243 LPYLRWLDLGGHEKEMKRTVKELDILFKGWLDEHKRKRLSGGKEDDDQD--FMDVMLSIL 302

Query: 307 DAKKLPRYYDGDTIIKATTLTMISGGTESSTVTLTWAMSLLLNNPHTLRRAYQELDEVVG 366
           +  KL    D DTI K   L +I GG +++  TLTWA+SLLLNNP+ L++A  ELD  VG
Sbjct: 303 EESKLGN--DVDTINKTACLAIILGGADTTWATLTWALSLLLNNPNALKKAQDELDLHVG 362

Query: 367 RDRRVNEADITKLVYLQAIVKETMRLSPAGPLLGPREFYKDCVVAGYFIPKGTQLIPNIW 426
           RDR V+E+D+ KL Y+ AI+KET+RL P GPLLGPR   +DC +AGY +  GT+LI N W
Sbjct: 363 RDRNVDESDLVKLTYIDAIIKETLRLYPPGPLLGPRVVTEDCTIAGYHVRAGTRLIVNAW 422

Query: 427 KIQTDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPAIAFGLQMVHFTL 486
           KIQ DP VW  P E++PERFL   +DVD+KG +FELIPFGSGRR CPAI+  LQ++  TL
Sbjct: 423 KIQRDPLVWSQPHEYQPERFL--ERDVDMKGQHFELIPFGSGRRACPAISLALQVLPLTL 482

Query: 487 AGFLQSFDIKNPTSEPVDMTENFGMANEKVVPLDVLVTPRL 524
           A  L  F+++ P    VDMTE  G+ + K  PL+VLV PR+
Sbjct: 483 AHILHGFELRTPNQNKVDMTETPGIVHAKATPLEVLVAPRI 513

BLAST of Tan0005335 vs. ExPASy Swiss-Prot
Match: Q9SZ46 (Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=1 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 6.4e-131
Identity = 239/516 (46.32%), Postives = 336/516 (65.12%), Query Frame = 0

Query: 16  LIIVLFVSCYFLWKKIGGVCEPK--KAPSPPGAWPIIGHLHLL-NKGKIPHHVLGAMADK 75
           +++ +F++ +   KK      PK  KAP+P GAWPIIGHLHLL  K ++ +  LG MAD 
Sbjct: 12  ILVFVFIALFKKSKK------PKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADH 71

Query: 76  YGPIFRLQLGSRSALVVSNWEMAKESLCTNDNAAASRPELSVSKHFSYDFAMFGLASYSP 135
           YGP   LQLGS  A VVS++E+AK+    ND A ASRP  + +KH  Y+FA+FG A YS 
Sbjct: 72  YGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAPYSA 131

Query: 136 YWRDMRKIANLELLSNPRVDQVKNIMFGEIDTSLTELHERWAVEKNESGQVPVEMKRWFG 195
           +WR+MRKIA +ELLSN R+  +K++   EI   + +L+  W  +   +  V V++K W  
Sbjct: 132 FWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLW-FKNGGTKPVMVDLKSWLE 191

Query: 196 DAIVNMLLKTIIGKRCVGPN---AMGGEKEAKELQMAIRDSFHLMGEGLLRDYIPVIGKL 255
           D  +NM+++ + GKR  G     +    +EA + + AI   FHL+G   + D  P +   
Sbjct: 192 DMTLNMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFF 251

Query: 256 GFDGNVKAMEKIAKRIDAIMAGWLEEHKRNRANGNLENKERDVNFMDSLLSLYDAKKLPR 315
              G+ K M++    +D I+  W+E H++ R       KE D +F+D ++SL +  KL  
Sbjct: 252 DLQGHEKEMKQTGSELDVILERWIENHRQQRKFSG--TKENDSDFIDVMMSLAEQGKLSH 311

Query: 316 -YYDGDTIIKATTLTMISGGTESSTVTLTWAMSLLLNNPHTLRRAYQELDEVVGRDRRVN 375
             YD +T IK+T L +I GG+++S  TLTWA+SLLLNN   L++A  E+D  VGRDR V 
Sbjct: 312 LQYDANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVE 371

Query: 376 EADITKLVYLQAIVKETMRLSPAGPLLGPREFYKDCVVAGYFIPKGTQLIPNIWKIQTDP 435
           ++DI  LVYLQAI+KET+RL PAGPLLGPRE  +DC VAGY++P GT+LI N+WKIQ DP
Sbjct: 372 DSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDP 431

Query: 436 RVWPDPFEFKPERFLTTH-KDVDLKGNNFELIPFGSGRRGCPAIAFGLQMVHFTLAGFLQ 495
           +V+ +P EF+PERF+T   K+ D++G NFEL+PFGSGRR CP  +  +Q++H  LA FL 
Sbjct: 432 KVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLH 491

Query: 496 SFDIKNPTSEPVDMTENFGMANEKVVPLDVLVTPRL 524
           SFD+K     PVDM+EN G+   K  PL+VL++PR+
Sbjct: 492 SFDVKTVMDMPVDMSENPGLTIPKATPLEVLISPRI 518

BLAST of Tan0005335 vs. NCBI nr
Match: XP_022976389.1 (cytochrome P450 82A3-like [Cucurbita maxima])

HSP 1 Score: 859.4 bits (2219), Expect = 1.7e-245
Identity = 419/531 (78.91%), Postives = 461/531 (86.82%), Query Frame = 0

Query: 1   MEFPLASLNPLSVATLIIVLFVSCYFLWKKI-GGVCEPKKAPSPPGAWPIIGHLHLLNKG 60
           MEFPL  LNPLS+  L I+   SCY   K +  GVCEPKKAPSPPGAWPIIGHLHLLN G
Sbjct: 1   MEFPLPYLNPLSIILLFII--SSCYLSRKWVKRGVCEPKKAPSPPGAWPIIGHLHLLNSG 60

Query: 61  KIPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKESLCTNDNAAASRPELSVSKHFS 120
           K+PHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKES+C ND AAASRPELSVSK+FS
Sbjct: 61  KLPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKESMCINDGAAASRPELSVSKNFS 120

Query: 121 YDFAMFGLASYSPYWRDMRKIANLELLSNPRVDQVKNIMFGEIDTSLTELHERWAVEKNE 180
           Y FAMFGLASY  YWRDMRKI +L+LLSNPRVDQVK++M  E+ TS+TELH RWA EKN+
Sbjct: 121 YHFAMFGLASYGTYWRDMRKITHLQLLSNPRVDQVKSMMLSEMATSVTELHARWASEKNQ 180

Query: 181 SGQVPVEMKRWFGDAIVNMLLKTIIGKRCVGPNAMGGEKEAKELQMAIRDSFHLMGEGLL 240
            G + VEMKRWFGD  VNMLLK IIGKRCVGPNA G EKEA +LQMAIR SFHLMGEGLL
Sbjct: 181 LGHIKVEMKRWFGDVTVNMLLKVIIGKRCVGPNAKGDEKEAIDLQMAIRKSFHLMGEGLL 240

Query: 241 RDYIPVIGKLGFDGNVKAMEKIAKRIDAIMAGWLEEHKRN-RANGNLENKERDVNFMDSL 300
           RDYIP++  +GFD +VKAMEKIAKRID+I+  WLEEH+RN   N +L+ K+ D  FMDSL
Sbjct: 241 RDYIPILANMGFDRHVKAMEKIAKRIDSILQCWLEEHRRNGDKNQSLDTKDGD--FMDSL 300

Query: 301 LSLYDAKKLPRYYDGDTIIKATTLTMISGGTESSTVTLTWAMSLLLNNPHTLRRAYQELD 360
           LSL  A +LP ++D DTI+KATTL MI+GGTESSTVTLTWA+SLL+NNP  L +AY+ELD
Sbjct: 301 LSLCRANELPSHFDHDTIVKATTLNMIAGGTESSTVTLTWALSLLVNNPRALEKAYEELD 360

Query: 361 EVVGRDRRVNEADITKLVYLQAIVKETMRLSPAGPLLGPREFYKDCVVAGYFIPKGTQLI 420
           +VVGRDRRV E+DITKLVYLQA+VKETMRL PAGPLLGPREFYKDC VAGYF+PKGTQLI
Sbjct: 361 QVVGRDRRVKESDITKLVYLQAVVKETMRLYPAGPLLGPREFYKDCFVAGYFVPKGTQLI 420

Query: 421 PNIWKIQTDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPAIAFGLQMV 480
           PNIWKIQTDPRVW DPFEFKPERFLTTH DVDLKGNNFELIPFGSGRRGCPA+AFGLQMV
Sbjct: 421 PNIWKIQTDPRVWADPFEFKPERFLTTHCDVDLKGNNFELIPFGSGRRGCPAVAFGLQMV 480

Query: 481 HFTLAGFLQSFDIKNPTSEPVDMTENFGMANEKVVPLDVLVTPRLPLHLHG 530
           HF LAGFL SF +K PT +PVDM ENFGMANEKVVPL+VLVTPRLP HLHG
Sbjct: 481 HFALAGFLHSFTVKTPTPQPVDMAENFGMANEKVVPLNVLVTPRLPSHLHG 527

BLAST of Tan0005335 vs. NCBI nr
Match: XP_023535454.1 (cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 847.8 bits (2189), Expect = 5.0e-242
Identity = 412/531 (77.59%), Postives = 457/531 (86.06%), Query Frame = 0

Query: 1   MEFPLASLNPLSVATLIIVLFVSCYFLWKKI-GGVCEPKKAPSPPGAWPIIGHLHLLNKG 60
           MEFPL  LNPLS+  L I+   SCY   K +  GVCEPKKAPSPPGAWPIIGHLHLLN G
Sbjct: 1   MEFPLPYLNPLSIILLFII--SSCYLSRKWVKRGVCEPKKAPSPPGAWPIIGHLHLLNNG 60

Query: 61  KIPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKESLCTNDNAAASRPELSVSKHFS 120
           K+PHHVLGAMADKYGPIF LQLGSR ALVVSNWEMAKES+C ND AAASRPELSVS++FS
Sbjct: 61  KLPHHVLGAMADKYGPIFHLQLGSRPALVVSNWEMAKESMCINDGAAASRPELSVSRNFS 120

Query: 121 YDFAMFGLASYSPYWRDMRKIANLELLSNPRVDQVKNIMFGEIDTSLTELHERWAVEKNE 180
           Y FAMFGLASYS YWRDMRKI +L LLSNP VDQVK++M  E+ TS+TELH RWA EKN+
Sbjct: 121 YHFAMFGLASYSSYWRDMRKITHLHLLSNPCVDQVKSMMLSEMATSVTELHARWASEKNQ 180

Query: 181 SGQVPVEMKRWFGDAIVNMLLKTIIGKRCVGPNAMGGEKEAKELQMAIRDSFHLMGEGLL 240
            G + VEMKRWFGD  +NMLLK IIGKRCVGPNA G EKEA +LQMAIR SFHLMGEG+L
Sbjct: 181 LGHIKVEMKRWFGDVTLNMLLKVIIGKRCVGPNAKGDEKEAIDLQMAIRKSFHLMGEGML 240

Query: 241 RDYIPVIGKLGFDGNVKAMEKIAKRIDAIMAGWLEEHKRNR-ANGNLENKERDVNFMDSL 300
           RDYIP++  LGFD + KAMEKIA RID+I+  WL+EH+RN   N +L+ K+ D  FMDSL
Sbjct: 241 RDYIPILANLGFDRHGKAMEKIAIRIDSILQSWLQEHRRNTDKNQSLDTKDGD--FMDSL 300

Query: 301 LSLYDAKKLPRYYDGDTIIKATTLTMISGGTESSTVTLTWAMSLLLNNPHTLRRAYQELD 360
           LSL  + +LP ++D DTI+KATTL MI+GGTESS+VTLTWA+SLL+NNP  L +AY+ELD
Sbjct: 301 LSLCRSNELPSHFDHDTIVKATTLNMIAGGTESSSVTLTWALSLLVNNPRALEKAYEELD 360

Query: 361 EVVGRDRRVNEADITKLVYLQAIVKETMRLSPAGPLLGPREFYKDCVVAGYFIPKGTQLI 420
           +VVGRDRRV E+DITKLVYLQA+VKETMRL PAGPLLGPREFYKDC VAGYF+PKGTQLI
Sbjct: 361 QVVGRDRRVKESDITKLVYLQAVVKETMRLYPAGPLLGPREFYKDCFVAGYFVPKGTQLI 420

Query: 421 PNIWKIQTDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPAIAFGLQMV 480
           PNIWKIQTDPRVWPDPFEFKPERFLTTH DVDLKGNNFELIPFGSGRRGCPA+AFGLQMV
Sbjct: 421 PNIWKIQTDPRVWPDPFEFKPERFLTTHCDVDLKGNNFELIPFGSGRRGCPAVAFGLQMV 480

Query: 481 HFTLAGFLQSFDIKNPTSEPVDMTENFGMANEKVVPLDVLVTPRLPLHLHG 530
           HF LAGFL SF +K PT +PVDM ENFGMANEKVVPLDVLVTPRLP HLHG
Sbjct: 481 HFALAGFLHSFTVKTPTPQPVDMAENFGMANEKVVPLDVLVTPRLPSHLHG 527

BLAST of Tan0005335 vs. NCBI nr
Match: XP_022935738.1 (cytochrome P450 82A3-like [Cucurbita moschata])

HSP 1 Score: 844.3 bits (2180), Expect = 5.5e-241
Identity = 411/531 (77.40%), Postives = 458/531 (86.25%), Query Frame = 0

Query: 1   MEFPLASLNPLSVATLIIVLFVSCYFLWKKI-GGVCEPKKAPSPPGAWPIIGHLHLLNKG 60
           MEFPL  LNPLS+  L I+   SCY   K +  GVCE KKAPSPPGAWPIIGHLHLLN G
Sbjct: 1   MEFPLPYLNPLSIILLFII--SSCYLSRKWVKRGVCEAKKAPSPPGAWPIIGHLHLLNNG 60

Query: 61  KIPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKESLCTNDNAAASRPELSVSKHFS 120
           K+PH VLGAMADKYGPIF LQLGSR ALVVSNWEMAKES+C ND AAASRPELSVSK+FS
Sbjct: 61  KLPHRVLGAMADKYGPIFHLQLGSRPALVVSNWEMAKESMCINDGAAASRPELSVSKNFS 120

Query: 121 YDFAMFGLASYSPYWRDMRKIANLELLSNPRVDQVKNIMFGEIDTSLTELHERWAVEKNE 180
           Y FAMFGLASYSPYWRDMRKI++L LLSN RVDQ K++M  E+ TS+TELH RWA EKN+
Sbjct: 121 YHFAMFGLASYSPYWRDMRKISHLHLLSNGRVDQAKSMMLSEMATSVTELHTRWASEKNQ 180

Query: 181 SGQVPVEMKRWFGDAIVNMLLKTIIGKRCVGPNAMGGEKEAKELQMAIRDSFHLMGEGLL 240
            G + VEMKRWFGD  +NMLLK IIGKRCVGPNA G EKEA +LQMAIR SFHLMGEG+L
Sbjct: 181 LGHIKVEMKRWFGDVTLNMLLKVIIGKRCVGPNAKGDEKEAIDLQMAIRKSFHLMGEGML 240

Query: 241 RDYIPVIGKLGFDGNVKAMEKIAKRIDAIMAGWLEEHKRNRA-NGNLENKERDVNFMDSL 300
           RDYIP++  LGFD +VKAMEKIA RID+I+ GWL+EH+RN+A N +L+ K+ D  FMDSL
Sbjct: 241 RDYIPILANLGFDRHVKAMEKIAIRIDSILQGWLQEHRRNKAKNQSLDTKDGD--FMDSL 300

Query: 301 LSLYDAKKLPRYYDGDTIIKATTLTMISGGTESSTVTLTWAMSLLLNNPHTLRRAYQELD 360
           LSL  + +LP  +D DTI+KAT L MI+GGTESS+VTLTWA+SLL+NNP  L +AY+ELD
Sbjct: 301 LSLCRSNELPSQFDHDTIVKATILNMIAGGTESSSVTLTWALSLLVNNPGALEKAYEELD 360

Query: 361 EVVGRDRRVNEADITKLVYLQAIVKETMRLSPAGPLLGPREFYKDCVVAGYFIPKGTQLI 420
           +VVGRDRRVNE+DITKLVYLQA+VKETMRL PAGPLLGPREFYKDC VAGYF+PKGTQLI
Sbjct: 361 QVVGRDRRVNESDITKLVYLQAVVKETMRLYPAGPLLGPREFYKDCFVAGYFVPKGTQLI 420

Query: 421 PNIWKIQTDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPAIAFGLQMV 480
           PNIWKIQTDPRVWPDPFEFKPERFLTTH DVDLKGNNFELIPFGSGRRGCPA+AFGLQMV
Sbjct: 421 PNIWKIQTDPRVWPDPFEFKPERFLTTHCDVDLKGNNFELIPFGSGRRGCPAVAFGLQMV 480

Query: 481 HFTLAGFLQSFDIKNPTSEPVDMTENFGMANEKVVPLDVLVTPRLPLHLHG 530
           HF LAG L SF +K PT +PVDM E+FGMANEKVVPL+VLVTPRLP HLHG
Sbjct: 481 HFALAGLLHSFTVKTPTPQPVDMAEHFGMANEKVVPLNVLVTPRLPSHLHG 527

BLAST of Tan0005335 vs. NCBI nr
Match: XP_038897047.1 (cytochrome P450 CYP82D47-like [Benincasa hispida])

HSP 1 Score: 843.2 bits (2177), Expect = 1.2e-240
Identity = 397/532 (74.62%), Postives = 455/532 (85.53%), Query Frame = 0

Query: 1   MEFPLASLNPLSVATLIIVLFVSCYFLWKKI--GGVCEPKKAPSPPGAWPIIGHLHLLNK 60
           MEF   S NP S+A L ++ F+SCY+LW+K+    V E K+AP  PGAWPIIGHLHL++ 
Sbjct: 2   MEFQFPSFNPESIAMLFVLFFISCYYLWRKLEQHNVSERKQAPVLPGAWPIIGHLHLMHN 61

Query: 61  GKIPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKESLCTNDNAAASRPELSVSKHF 120
            K+PHH LGAMADKYGPIFRLQLGSRSALVVS+WEMAKES+C ND AAASRP +S +KHF
Sbjct: 62  AKLPHHALGAMADKYGPIFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKHF 121

Query: 121 SYDFAMFGLASYSPYWRDMRKIANLELLSNPRVDQVKNIMFGEIDTSLTELHERWAVEKN 180
           SYDFA FGLA YSPYWR++RK+ ++ELLSN RVDQ KN MFGEI TSLTELH+ WA +K+
Sbjct: 122 SYDFAAFGLAPYSPYWREIRKVTHMELLSNQRVDQFKNTMFGEIKTSLTELHKTWAAQKD 181

Query: 181 ESGQVPVEMKRWFGDAIVNMLLKTIIGKRCVGPNAMGGEKEAKELQMAIRDSFHLMGEGL 240
           ESGQV VEMKRWFGD I+NMLLK IIGKRCVGPNA GGEK+AK+ Q+AIRDSFHLMG+GL
Sbjct: 182 ESGQVQVEMKRWFGDVILNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGL 241

Query: 241 LRDYIPVIGKLGFDGNVKAMEKIAKRIDAIMAGWLEEHKRNRANGNLENKERDVNFMDSL 300
           LRDYIP+IG+LGF+G VK ME IA R D ++  WLEEHKR RA+ +   K  D  FMD+L
Sbjct: 242 LRDYIPLIGRLGFNGQVKVMENIATRFDMVLREWLEEHKRKRASSSSGKKNED--FMDAL 301

Query: 301 LSLYDAKKLPRYYDGDTIIKATTLTMISGGTESSTVTLTWAMSLLLNNPHTLRRAYQELD 360
           LSLYD K++  YYDGDTIIKATTL MI+GGTES+TVTLTWAMSLLLNNPH L +A QELD
Sbjct: 302 LSLYDGKRIEGYYDGDTIIKATTLNMIAGGTESTTVTLTWAMSLLLNNPHVLEKAQQELD 361

Query: 361 EVVGRDRRVNEADITKLVYLQAIVKETMRLSPAGPLLGPREFYKDCVVAGYFIPKGTQLI 420
            VVGRDRR+ ++DI  LVYL  I+KETMRL PAGPLLGPREFYKDC+V GYF+PKGTQLI
Sbjct: 362 TVVGRDRRLKDSDIPNLVYLHCIIKETMRLYPAGPLLGPREFYKDCIVDGYFVPKGTQLI 421

Query: 421 PNIWKIQTDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPAIAFGLQMV 480
           PNIWKIQTDPRVWPDPFEFKPERFLTTHK+VDLKGNNFELIPFG+GRRGCP I+FGLQMV
Sbjct: 422 PNIWKIQTDPRVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGTGRRGCPGISFGLQMV 481

Query: 481 HFTLAGFLQSFDIKNPTSEPVDMTENFGMANEKVVPLDVLVTPRLPLHLHGI 531
           HF LAGFL SFD++ PT EP+DM+ENFGMANEKVVPL+VLVT RLP +L+ +
Sbjct: 482 HFALAGFLHSFDVRKPTKEPIDMSENFGMANEKVVPLNVLVTSRLPSYLYAL 531

BLAST of Tan0005335 vs. NCBI nr
Match: XP_022936278.1 (cytochrome P450 CYP82D47-like [Cucurbita moschata])

HSP 1 Score: 842.8 bits (2176), Expect = 1.6e-240
Identity = 409/531 (77.02%), Postives = 456/531 (85.88%), Query Frame = 0

Query: 1   MEFPLASLNPLSVATLIIVLFVSCYFLWKKI-GGVCEPKKAPSPPGAWPIIGHLHLLNKG 60
           MEFPL  LNPLS+  L I+   SCY   K +  GVCE KK PSPPGAWPIIGHLHLLN G
Sbjct: 1   MEFPLPYLNPLSIILLFII--SSCYLSRKWVKRGVCEAKKVPSPPGAWPIIGHLHLLNNG 60

Query: 61  KIPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKESLCTNDNAAASRPELSVSKHFS 120
           K+PH VLGAMADKYGPIF LQ+GSR ALVVSNWEMAKES+C ND AAASRPELSVSK+FS
Sbjct: 61  KLPHRVLGAMADKYGPIFHLQIGSRPALVVSNWEMAKESMCINDGAAASRPELSVSKNFS 120

Query: 121 YDFAMFGLASYSPYWRDMRKIANLELLSNPRVDQVKNIMFGEIDTSLTELHERWAVEKNE 180
           Y FAMFGLASYSPYWRDMRKI++L LLSN RVDQVK++M  E+ TS+TELH RWA EKN+
Sbjct: 121 YHFAMFGLASYSPYWRDMRKISHLHLLSNRRVDQVKSMMLSEMATSVTELHARWASEKNQ 180

Query: 181 SGQVPVEMKRWFGDAIVNMLLKTIIGKRCVGPNAMGGEKEAKELQMAIRDSFHLMGEGLL 240
            G + VEMKRWFGD I NMLLK IIGKRCVGPNA G EKEA +LQMAI  SFHLMGEG+L
Sbjct: 181 MGHINVEMKRWFGDVIANMLLKVIIGKRCVGPNAKGDEKEAIDLQMAIGKSFHLMGEGML 240

Query: 241 RDYIPVIGKLGFDGNVKAMEKIAKRIDAIMAGWLEEHKRNR-ANGNLENKERDVNFMDSL 300
           RDYIP++  LGFD + KAMEKIA RID+I+ GWL+EH+RNR  N +L+ K+ D  FMDSL
Sbjct: 241 RDYIPILANLGFDRHSKAMEKIATRIDSILQGWLQEHRRNRDKNQSLDTKDGD--FMDSL 300

Query: 301 LSLYDAKKLPRYYDGDTIIKATTLTMISGGTESSTVTLTWAMSLLLNNPHTLRRAYQELD 360
           LSL    +LPR++D DTI+KATTL MI+GGTE+STVTLTWA+SLL+NNP  L +AY+ELD
Sbjct: 301 LSLCRTNELPRHFDHDTIVKATTLNMIAGGTETSTVTLTWALSLLVNNPGALEKAYEELD 360

Query: 361 EVVGRDRRVNEADITKLVYLQAIVKETMRLSPAGPLLGPREFYKDCVVAGYFIPKGTQLI 420
           +VVGRDRRVNE+DITKLVYLQA+VKETMRL PAGPLLGPREFYKDC VAGYF+PKGTQLI
Sbjct: 361 QVVGRDRRVNESDITKLVYLQAVVKETMRLYPAGPLLGPREFYKDCFVAGYFVPKGTQLI 420

Query: 421 PNIWKIQTDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPAIAFGLQMV 480
           PNIWKIQTDPRVWPDPFEFKPERFLTTH DVDLKGNNFELIPFGSGRRGCP IAFGLQMV
Sbjct: 421 PNIWKIQTDPRVWPDPFEFKPERFLTTHCDVDLKGNNFELIPFGSGRRGCPGIAFGLQMV 480

Query: 481 HFTLAGFLQSFDIKNPTSEPVDMTENFGMANEKVVPLDVLVTPRLPLHLHG 530
           HF LAGFL SF++K PT + +DM E FGMANEK+VPL+VLVTPRLP HLHG
Sbjct: 481 HFALAGFLHSFNVKTPTPQSIDMAEGFGMANEKLVPLNVLVTPRLPSHLHG 527

BLAST of Tan0005335 vs. ExPASy TrEMBL
Match: A0A6J1IND1 (cytochrome P450 82A3-like OS=Cucurbita maxima OX=3661 GN=LOC111476807 PE=3 SV=1)

HSP 1 Score: 859.4 bits (2219), Expect = 8.1e-246
Identity = 419/531 (78.91%), Postives = 461/531 (86.82%), Query Frame = 0

Query: 1   MEFPLASLNPLSVATLIIVLFVSCYFLWKKI-GGVCEPKKAPSPPGAWPIIGHLHLLNKG 60
           MEFPL  LNPLS+  L I+   SCY   K +  GVCEPKKAPSPPGAWPIIGHLHLLN G
Sbjct: 1   MEFPLPYLNPLSIILLFII--SSCYLSRKWVKRGVCEPKKAPSPPGAWPIIGHLHLLNSG 60

Query: 61  KIPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKESLCTNDNAAASRPELSVSKHFS 120
           K+PHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKES+C ND AAASRPELSVSK+FS
Sbjct: 61  KLPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKESMCINDGAAASRPELSVSKNFS 120

Query: 121 YDFAMFGLASYSPYWRDMRKIANLELLSNPRVDQVKNIMFGEIDTSLTELHERWAVEKNE 180
           Y FAMFGLASY  YWRDMRKI +L+LLSNPRVDQVK++M  E+ TS+TELH RWA EKN+
Sbjct: 121 YHFAMFGLASYGTYWRDMRKITHLQLLSNPRVDQVKSMMLSEMATSVTELHARWASEKNQ 180

Query: 181 SGQVPVEMKRWFGDAIVNMLLKTIIGKRCVGPNAMGGEKEAKELQMAIRDSFHLMGEGLL 240
            G + VEMKRWFGD  VNMLLK IIGKRCVGPNA G EKEA +LQMAIR SFHLMGEGLL
Sbjct: 181 LGHIKVEMKRWFGDVTVNMLLKVIIGKRCVGPNAKGDEKEAIDLQMAIRKSFHLMGEGLL 240

Query: 241 RDYIPVIGKLGFDGNVKAMEKIAKRIDAIMAGWLEEHKRN-RANGNLENKERDVNFMDSL 300
           RDYIP++  +GFD +VKAMEKIAKRID+I+  WLEEH+RN   N +L+ K+ D  FMDSL
Sbjct: 241 RDYIPILANMGFDRHVKAMEKIAKRIDSILQCWLEEHRRNGDKNQSLDTKDGD--FMDSL 300

Query: 301 LSLYDAKKLPRYYDGDTIIKATTLTMISGGTESSTVTLTWAMSLLLNNPHTLRRAYQELD 360
           LSL  A +LP ++D DTI+KATTL MI+GGTESSTVTLTWA+SLL+NNP  L +AY+ELD
Sbjct: 301 LSLCRANELPSHFDHDTIVKATTLNMIAGGTESSTVTLTWALSLLVNNPRALEKAYEELD 360

Query: 361 EVVGRDRRVNEADITKLVYLQAIVKETMRLSPAGPLLGPREFYKDCVVAGYFIPKGTQLI 420
           +VVGRDRRV E+DITKLVYLQA+VKETMRL PAGPLLGPREFYKDC VAGYF+PKGTQLI
Sbjct: 361 QVVGRDRRVKESDITKLVYLQAVVKETMRLYPAGPLLGPREFYKDCFVAGYFVPKGTQLI 420

Query: 421 PNIWKIQTDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPAIAFGLQMV 480
           PNIWKIQTDPRVW DPFEFKPERFLTTH DVDLKGNNFELIPFGSGRRGCPA+AFGLQMV
Sbjct: 421 PNIWKIQTDPRVWADPFEFKPERFLTTHCDVDLKGNNFELIPFGSGRRGCPAVAFGLQMV 480

Query: 481 HFTLAGFLQSFDIKNPTSEPVDMTENFGMANEKVVPLDVLVTPRLPLHLHG 530
           HF LAGFL SF +K PT +PVDM ENFGMANEKVVPL+VLVTPRLP HLHG
Sbjct: 481 HFALAGFLHSFTVKTPTPQPVDMAENFGMANEKVVPLNVLVTPRLPSHLHG 527

BLAST of Tan0005335 vs. ExPASy TrEMBL
Match: A0A6J1FBC6 (cytochrome P450 82A3-like OS=Cucurbita moschata OX=3662 GN=LOC111442561 PE=3 SV=1)

HSP 1 Score: 844.3 bits (2180), Expect = 2.7e-241
Identity = 411/531 (77.40%), Postives = 458/531 (86.25%), Query Frame = 0

Query: 1   MEFPLASLNPLSVATLIIVLFVSCYFLWKKI-GGVCEPKKAPSPPGAWPIIGHLHLLNKG 60
           MEFPL  LNPLS+  L I+   SCY   K +  GVCE KKAPSPPGAWPIIGHLHLLN G
Sbjct: 1   MEFPLPYLNPLSIILLFII--SSCYLSRKWVKRGVCEAKKAPSPPGAWPIIGHLHLLNNG 60

Query: 61  KIPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKESLCTNDNAAASRPELSVSKHFS 120
           K+PH VLGAMADKYGPIF LQLGSR ALVVSNWEMAKES+C ND AAASRPELSVSK+FS
Sbjct: 61  KLPHRVLGAMADKYGPIFHLQLGSRPALVVSNWEMAKESMCINDGAAASRPELSVSKNFS 120

Query: 121 YDFAMFGLASYSPYWRDMRKIANLELLSNPRVDQVKNIMFGEIDTSLTELHERWAVEKNE 180
           Y FAMFGLASYSPYWRDMRKI++L LLSN RVDQ K++M  E+ TS+TELH RWA EKN+
Sbjct: 121 YHFAMFGLASYSPYWRDMRKISHLHLLSNGRVDQAKSMMLSEMATSVTELHTRWASEKNQ 180

Query: 181 SGQVPVEMKRWFGDAIVNMLLKTIIGKRCVGPNAMGGEKEAKELQMAIRDSFHLMGEGLL 240
            G + VEMKRWFGD  +NMLLK IIGKRCVGPNA G EKEA +LQMAIR SFHLMGEG+L
Sbjct: 181 LGHIKVEMKRWFGDVTLNMLLKVIIGKRCVGPNAKGDEKEAIDLQMAIRKSFHLMGEGML 240

Query: 241 RDYIPVIGKLGFDGNVKAMEKIAKRIDAIMAGWLEEHKRNRA-NGNLENKERDVNFMDSL 300
           RDYIP++  LGFD +VKAMEKIA RID+I+ GWL+EH+RN+A N +L+ K+ D  FMDSL
Sbjct: 241 RDYIPILANLGFDRHVKAMEKIAIRIDSILQGWLQEHRRNKAKNQSLDTKDGD--FMDSL 300

Query: 301 LSLYDAKKLPRYYDGDTIIKATTLTMISGGTESSTVTLTWAMSLLLNNPHTLRRAYQELD 360
           LSL  + +LP  +D DTI+KAT L MI+GGTESS+VTLTWA+SLL+NNP  L +AY+ELD
Sbjct: 301 LSLCRSNELPSQFDHDTIVKATILNMIAGGTESSSVTLTWALSLLVNNPGALEKAYEELD 360

Query: 361 EVVGRDRRVNEADITKLVYLQAIVKETMRLSPAGPLLGPREFYKDCVVAGYFIPKGTQLI 420
           +VVGRDRRVNE+DITKLVYLQA+VKETMRL PAGPLLGPREFYKDC VAGYF+PKGTQLI
Sbjct: 361 QVVGRDRRVNESDITKLVYLQAVVKETMRLYPAGPLLGPREFYKDCFVAGYFVPKGTQLI 420

Query: 421 PNIWKIQTDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPAIAFGLQMV 480
           PNIWKIQTDPRVWPDPFEFKPERFLTTH DVDLKGNNFELIPFGSGRRGCPA+AFGLQMV
Sbjct: 421 PNIWKIQTDPRVWPDPFEFKPERFLTTHCDVDLKGNNFELIPFGSGRRGCPAVAFGLQMV 480

Query: 481 HFTLAGFLQSFDIKNPTSEPVDMTENFGMANEKVVPLDVLVTPRLPLHLHG 530
           HF LAG L SF +K PT +PVDM E+FGMANEKVVPL+VLVTPRLP HLHG
Sbjct: 481 HFALAGLLHSFTVKTPTPQPVDMAEHFGMANEKVVPLNVLVTPRLPSHLHG 527

BLAST of Tan0005335 vs. ExPASy TrEMBL
Match: A0A6J1D746 (cytochrome P450 82A3-like OS=Momordica charantia OX=3673 GN=LOC111017857 PE=3 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 7.8e-241
Identity = 402/522 (77.01%), Postives = 456/522 (87.36%), Query Frame = 0

Query: 10  PLSVATLIIVLFVSCYFLWKKIGGVCEPKKAPSPPGAWPIIGHLHLLNKGKIPHHVLGAM 69
           P+ +A + ++  V  Y+L +K     + K APSPPGAWP+IGHLHLL+  K+PHH LGAM
Sbjct: 16  PICIAAIFLIFIVCYYYLCRKRQD--QRKLAPSPPGAWPVIGHLHLLHNAKLPHHTLGAM 75

Query: 70  ADKYGPIFRLQLGSRSALVVSNWEMAKESLCTNDNAAASRPELSVSKHFSYDFAMFGLAS 129
           ADKYGPIFRLQLGSRSALVVSNWEMAKES+C ND AAASRPELSVSKHFSYDFAMFGLAS
Sbjct: 76  ADKYGPIFRLQLGSRSALVVSNWEMAKESMCINDAAAASRPELSVSKHFSYDFAMFGLAS 135

Query: 130 YSPYWRDMRKIANLELLSNPRVDQVKNIMFGEIDTSLTELHERWAVEKNESGQVPVEMKR 189
           Y PYWR+MRKI +LELLSNPRV +VKNIMFGE+D SLTELH +W V++NESGQ+PVE+K 
Sbjct: 136 YGPYWREMRKITHLELLSNPRVQEVKNIMFGEVDMSLTELHAKWVVQRNESGQIPVELKG 195

Query: 190 WFGDAIVNMLLKTIIGKRCVGPNAMGGEKEAKELQMAIRDSFHLMGEGLLRDYIPVIGKL 249
           WFGD ++NMLLK IIGKRCVGPNA+GGE EAKELQMAIR+SF LMG+G+LRD+IPV+ +L
Sbjct: 196 WFGDVVMNMLLKIIIGKRCVGPNALGGEAEAKELQMAIRESFRLMGQGILRDFIPVVAQL 255

Query: 250 GFDGNVKAMEKIAKRIDAIMAGWLEEHKRNRANGNLENKERDVNFMDSLLSLYDAKKLPR 309
           GFDG VKAMEKIAKRIDAI+ GWL++HK+NRA G L  K  D  FM SLLSLYDA +LP 
Sbjct: 256 GFDGQVKAMEKIAKRIDAILEGWLQDHKKNRAYG-LAKKNGD--FMVSLLSLYDANQLPS 315

Query: 310 YYDGDTIIKATTLTMISGGTESSTVTLTWAMSLLLNNPHTLRRAYQELDEVVGRDRRVNE 369
            Y+GD I KATTL MI+GGTESSTVTLTWAMSLLLNNPH L +AYQELD VVGRDRRVNE
Sbjct: 316 QYNGDIITKATTLNMIAGGTESSTVTLTWAMSLLLNNPHALEKAYQELDAVVGRDRRVNE 375

Query: 370 ADITKLVYLQAIVKETMRLSPAGPLLGPREFYKDCVVAGYFIPKGTQLIPNIWKIQTDPR 429
           +DI+KL YLQAIVKETMRL PAGPLLGPREFYKDCVV GYF+PKGTQLIPNIWKIQ DPR
Sbjct: 376 SDISKLSYLQAIVKETMRLYPAGPLLGPREFYKDCVVGGYFVPKGTQLIPNIWKIQVDPR 435

Query: 430 VWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPAIAFGLQMVHFTLAGFLQSF 489
           VWPDP +FKPERFLTTHK+VD+KGN+FELIPFGSGRRGCP +AFGLQMVHFTLAGFLQSF
Sbjct: 436 VWPDPLKFKPERFLTTHKEVDVKGNDFELIPFGSGRRGCPGVAFGLQMVHFTLAGFLQSF 495

Query: 490 DIKNPTSEPVDMTENFGMANEKVVPLDVLVTPRLPLHLHGIV 532
           D+KN TS  +DM+E FGMANEK +PL+V+VTPRL  HLH ++
Sbjct: 496 DVKNVTSNTIDMSEVFGMANEKFLPLNVVVTPRLLSHLHTLI 532

BLAST of Tan0005335 vs. ExPASy TrEMBL
Match: A0A6J1FCU2 (cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442946 PE=3 SV=1)

HSP 1 Score: 842.8 bits (2176), Expect = 7.8e-241
Identity = 409/531 (77.02%), Postives = 456/531 (85.88%), Query Frame = 0

Query: 1   MEFPLASLNPLSVATLIIVLFVSCYFLWKKI-GGVCEPKKAPSPPGAWPIIGHLHLLNKG 60
           MEFPL  LNPLS+  L I+   SCY   K +  GVCE KK PSPPGAWPIIGHLHLLN G
Sbjct: 1   MEFPLPYLNPLSIILLFII--SSCYLSRKWVKRGVCEAKKVPSPPGAWPIIGHLHLLNNG 60

Query: 61  KIPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKESLCTNDNAAASRPELSVSKHFS 120
           K+PH VLGAMADKYGPIF LQ+GSR ALVVSNWEMAKES+C ND AAASRPELSVSK+FS
Sbjct: 61  KLPHRVLGAMADKYGPIFHLQIGSRPALVVSNWEMAKESMCINDGAAASRPELSVSKNFS 120

Query: 121 YDFAMFGLASYSPYWRDMRKIANLELLSNPRVDQVKNIMFGEIDTSLTELHERWAVEKNE 180
           Y FAMFGLASYSPYWRDMRKI++L LLSN RVDQVK++M  E+ TS+TELH RWA EKN+
Sbjct: 121 YHFAMFGLASYSPYWRDMRKISHLHLLSNRRVDQVKSMMLSEMATSVTELHARWASEKNQ 180

Query: 181 SGQVPVEMKRWFGDAIVNMLLKTIIGKRCVGPNAMGGEKEAKELQMAIRDSFHLMGEGLL 240
            G + VEMKRWFGD I NMLLK IIGKRCVGPNA G EKEA +LQMAI  SFHLMGEG+L
Sbjct: 181 MGHINVEMKRWFGDVIANMLLKVIIGKRCVGPNAKGDEKEAIDLQMAIGKSFHLMGEGML 240

Query: 241 RDYIPVIGKLGFDGNVKAMEKIAKRIDAIMAGWLEEHKRNR-ANGNLENKERDVNFMDSL 300
           RDYIP++  LGFD + KAMEKIA RID+I+ GWL+EH+RNR  N +L+ K+ D  FMDSL
Sbjct: 241 RDYIPILANLGFDRHSKAMEKIATRIDSILQGWLQEHRRNRDKNQSLDTKDGD--FMDSL 300

Query: 301 LSLYDAKKLPRYYDGDTIIKATTLTMISGGTESSTVTLTWAMSLLLNNPHTLRRAYQELD 360
           LSL    +LPR++D DTI+KATTL MI+GGTE+STVTLTWA+SLL+NNP  L +AY+ELD
Sbjct: 301 LSLCRTNELPRHFDHDTIVKATTLNMIAGGTETSTVTLTWALSLLVNNPGALEKAYEELD 360

Query: 361 EVVGRDRRVNEADITKLVYLQAIVKETMRLSPAGPLLGPREFYKDCVVAGYFIPKGTQLI 420
           +VVGRDRRVNE+DITKLVYLQA+VKETMRL PAGPLLGPREFYKDC VAGYF+PKGTQLI
Sbjct: 361 QVVGRDRRVNESDITKLVYLQAVVKETMRLYPAGPLLGPREFYKDCFVAGYFVPKGTQLI 420

Query: 421 PNIWKIQTDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPAIAFGLQMV 480
           PNIWKIQTDPRVWPDPFEFKPERFLTTH DVDLKGNNFELIPFGSGRRGCP IAFGLQMV
Sbjct: 421 PNIWKIQTDPRVWPDPFEFKPERFLTTHCDVDLKGNNFELIPFGSGRRGCPGIAFGLQMV 480

Query: 481 HFTLAGFLQSFDIKNPTSEPVDMTENFGMANEKVVPLDVLVTPRLPLHLHG 530
           HF LAGFL SF++K PT + +DM E FGMANEK+VPL+VLVTPRLP HLHG
Sbjct: 481 HFALAGFLHSFNVKTPTPQSIDMAEGFGMANEKLVPLNVLVTPRLPSHLHG 527

BLAST of Tan0005335 vs. ExPASy TrEMBL
Match: A0A0A0LD43 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G852590 PE=3 SV=1)

HSP 1 Score: 841.3 bits (2172), Expect = 2.3e-240
Identity = 394/532 (74.06%), Postives = 455/532 (85.53%), Query Frame = 0

Query: 1   MEFPLASLNPLSVATLIIVLFVSCYFLWKKIGGV--CEPKKAPSPPGAWPIIGHLHLLNK 60
           +EF     NP S+  L+ + F+SCY+LW+K       EPK+AP+PPGAWPIIGHLH+L+ 
Sbjct: 2   VEFQFPFFNPKSITMLVFLFFISCYYLWQKWQQYKSTEPKQAPAPPGAWPIIGHLHMLHN 61

Query: 61  GKIPHHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKESLCTNDNAAASRPELSVSKHF 120
            K+PHH LGAMADKYGP+FRLQLGSRSALVVS+WEMAKES+C ND AAASRP +S +KHF
Sbjct: 62  VKLPHHALGAMADKYGPLFRLQLGSRSALVVSSWEMAKESMCVNDAAAASRPGVSGTKHF 121

Query: 121 SYDFAMFGLASYSPYWRDMRKIANLELLSNPRVDQVKNIMFGEIDTSLTELHERWAVEKN 180
           SYDFA FGLA YSPYWR++RK+ ++ELLSNPRVDQ KN MFGE+ TSL ELHE WA +K+
Sbjct: 122 SYDFAAFGLAPYSPYWREIRKVTHMELLSNPRVDQFKNTMFGEVKTSLRELHETWAAQKD 181

Query: 181 ESGQVPVEMKRWFGDAIVNMLLKTIIGKRCVGPNAMGGEKEAKELQMAIRDSFHLMGEGL 240
            SGQV VEMKRWFGD IVNMLLK IIGKRCVGPNA GGEK+AK+ Q+AIRDSFHLMG+GL
Sbjct: 182 GSGQVEVEMKRWFGDVIVNMLLKIIIGKRCVGPNAEGGEKQAKDFQLAIRDSFHLMGQGL 241

Query: 241 LRDYIPVIGKLGFDGNVKAMEKIAKRIDAIMAGWLEEHKRNRANGNLENKERDVNFMDSL 300
           LRDYIP+IG+LGF+G VK ME IA R D ++  WL+EHK NR +     K+ D  FMD+L
Sbjct: 242 LRDYIPLIGRLGFNGQVKVMENIATRFDMVLREWLDEHKLNRTSSCCGRKDGD--FMDAL 301

Query: 301 LSLYDAKKLPRYYDGDTIIKATTLTMISGGTESSTVTLTWAMSLLLNNPHTLRRAYQELD 360
           +SLYD K++  YYDGDTIIKATTL M++GGTES+TVTLTWAMSLL+NNPH L RA QELD
Sbjct: 302 VSLYDGKEIEGYYDGDTIIKATTLNMVAGGTESTTVTLTWAMSLLINNPHVLERAQQELD 361

Query: 361 EVVGRDRRVNEADITKLVYLQAIVKETMRLSPAGPLLGPREFYKDCVVAGYFIPKGTQLI 420
            VVGRDR++ E+DI  LVYL++I+KETMR+ PAGPLLGPREFYKDC+VAGYF+PKGTQLI
Sbjct: 362 TVVGRDRQLKESDIPNLVYLKSIIKETMRMYPAGPLLGPREFYKDCIVAGYFVPKGTQLI 421

Query: 421 PNIWKIQTDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPAIAFGLQMV 480
           PNIWKIQTDPRVWPDPFEFKPERFLTTHK+VDLKGNNFELIPFGSGRRGCP +AFGLQMV
Sbjct: 422 PNIWKIQTDPRVWPDPFEFKPERFLTTHKNVDLKGNNFELIPFGSGRRGCPGLAFGLQMV 481

Query: 481 HFTLAGFLQSFDIKNPTSEPVDMTENFGMANEKVVPLDVLVTPRLPLHLHGI 531
           HF LAGFL SFD+KNPT EP+DM+ENFGMANEKVVPL+V VT RLP HL+ I
Sbjct: 482 HFALAGFLHSFDVKNPTKEPIDMSENFGMANEKVVPLNVSVTSRLPSHLYTI 531

BLAST of Tan0005335 vs. TAIR 10
Match: AT4G31940.1 (cytochrome P450, family 82, subfamily C, polypeptide 4 )

HSP 1 Score: 469.2 bits (1206), Expect = 4.5e-132
Identity = 239/516 (46.32%), Postives = 336/516 (65.12%), Query Frame = 0

Query: 16  LIIVLFVSCYFLWKKIGGVCEPK--KAPSPPGAWPIIGHLHLL-NKGKIPHHVLGAMADK 75
           +++ +F++ +   KK      PK  KAP+P GAWPIIGHLHLL  K ++ +  LG MAD 
Sbjct: 12  ILVFVFIALFKKSKK------PKYVKAPAPSGAWPIIGHLHLLGGKEQLLYRTLGKMADH 71

Query: 76  YGPIFRLQLGSRSALVVSNWEMAKESLCTNDNAAASRPELSVSKHFSYDFAMFGLASYSP 135
           YGP   LQLGS  A VVS++E+AK+    ND A ASRP  + +KH  Y+FA+FG A YS 
Sbjct: 72  YGPAMSLQLGSNEAFVVSSFEVAKDCFTVNDKALASRPMTAAAKHMGYNFAVFGFAPYSA 131

Query: 136 YWRDMRKIANLELLSNPRVDQVKNIMFGEIDTSLTELHERWAVEKNESGQVPVEMKRWFG 195
           +WR+MRKIA +ELLSN R+  +K++   EI   + +L+  W  +   +  V V++K W  
Sbjct: 132 FWREMRKIATIELLSNRRLQMLKHVRVSEITMGVKDLYSLW-FKNGGTKPVMVDLKSWLE 191

Query: 196 DAIVNMLLKTIIGKRCVGPN---AMGGEKEAKELQMAIRDSFHLMGEGLLRDYIPVIGKL 255
           D  +NM+++ + GKR  G     +    +EA + + AI   FHL+G   + D  P +   
Sbjct: 192 DMTLNMIVRMVAGKRYFGGGGSVSSEDTEEAMQCKKAIAKFFHLIGIFTVSDAFPTLSFF 251

Query: 256 GFDGNVKAMEKIAKRIDAIMAGWLEEHKRNRANGNLENKERDVNFMDSLLSLYDAKKLPR 315
              G+ K M++    +D I+  W+E H++ R       KE D +F+D ++SL +  KL  
Sbjct: 252 DLQGHEKEMKQTGSELDVILERWIENHRQQRKFSG--TKENDSDFIDVMMSLAEQGKLSH 311

Query: 316 -YYDGDTIIKATTLTMISGGTESSTVTLTWAMSLLLNNPHTLRRAYQELDEVVGRDRRVN 375
             YD +T IK+T L +I GG+++S  TLTWA+SLLLNN   L++A  E+D  VGRDR V 
Sbjct: 312 LQYDANTSIKSTCLALILGGSDTSASTLTWAISLLLNNKEMLKKAQDEIDIHVGRDRNVE 371

Query: 376 EADITKLVYLQAIVKETMRLSPAGPLLGPREFYKDCVVAGYFIPKGTQLIPNIWKIQTDP 435
           ++DI  LVYLQAI+KET+RL PAGPLLGPRE  +DC VAGY++P GT+LI N+WKIQ DP
Sbjct: 372 DSDIENLVYLQAIIKETLRLYPAGPLLGPREAMEDCTVAGYYVPCGTRLIVNVWKIQRDP 431

Query: 436 RVWPDPFEFKPERFLTTH-KDVDLKGNNFELIPFGSGRRGCPAIAFGLQMVHFTLAGFLQ 495
           +V+ +P EF+PERF+T   K+ D++G NFEL+PFGSGRR CP  +  +Q++H  LA FL 
Sbjct: 432 KVYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLH 491

Query: 496 SFDIKNPTSEPVDMTENFGMANEKVVPLDVLVTPRL 524
           SFD+K     PVDM+EN G+   K  PL+VL++PR+
Sbjct: 492 SFDVKTVMDMPVDMSENPGLTIPKATPLEVLISPRI 518

BLAST of Tan0005335 vs. TAIR 10
Match: AT4G31970.1 (cytochrome P450, family 82, subfamily C, polypeptide 2 )

HSP 1 Score: 467.2 bits (1201), Expect = 1.7e-131
Identity = 238/515 (46.21%), Postives = 340/515 (66.02%), Query Frame = 0

Query: 16  LIIVLFVSCYFLWKKIGGVCEPK--KAPSPPGAWPIIGHLHLLN-KGKIPHHVLGAMADK 75
           +++ +F++ +   KK      PK  KAP+P GAWPIIGHLHLL+ K ++ +  LG MAD+
Sbjct: 12  ILVFVFIALFKKSKK------PKHVKAPAPSGAWPIIGHLHLLSGKEQLLYRTLGKMADQ 71

Query: 76  YGPIFRLQLGSRSALVVSNWEMAKESLCTNDNAAASRPELSVSKHFSYDFAMFGLASYSP 135
           YGP   L+LGS    VVS++E+AK+    ND A ASRP  + +KH  YD A+FG A YS 
Sbjct: 72  YGPAMSLRLGSSETFVVSSFEVAKDCFTVNDKALASRPITAAAKHMGYDCAVFGFAPYSA 131

Query: 136 YWRDMRKIANLELLSNPRVDQVKNIMFGEIDTSLTELHERWAVEKNESGQVPVEMKRWFG 195
           +WR+MRKIA LELLSN R+  +K++   EI   + +L+  W V+K  S  V V++K W  
Sbjct: 132 FWREMRKIATLELLSNRRLQMLKHVRVSEISMVMQDLYSLW-VKKGGSEPVMVDLKSWLE 191

Query: 196 DAIVNMLLKTIIGKRCVGPNAMGGE--KEAKELQMAIRDSFHLMGEGLLRDYIPVIGKLG 255
           D  +NM+++ + GKR  G  ++  E  +EA++ +  + + FHL+G   + D  P +G   
Sbjct: 192 DMSLNMMVRMVAGKRYFGGGSLSPEDAEEARQCRKGVANFFHLVGIFTVSDAFPKLGWFD 251

Query: 256 FDGNVKAMEKIAKRIDAIMAGWLEEHKRNRANGNLENKERDVNFMDSLLSLYDAKKLPR- 315
           F G+ K M++  + +D I+  W+E H++ R       K  D +F+D +LSL +  K    
Sbjct: 252 FQGHEKEMKQTGRELDVILERWIENHRQQRKVSG--TKHNDSDFVDVMLSLAEQGKFSHL 311

Query: 316 YYDGDTIIKATTLTMISGGTESSTVTLTWAMSLLLNNPHTLRRAYQELDEVVGRDRRVNE 375
            +D  T IK+T L +I GG+E+S  TLTWA+SLLLNN   L++A  E+D  VGRDR V +
Sbjct: 312 QHDAITSIKSTCLALILGGSETSPSTLTWAISLLLNNKDMLKKAQDEIDIHVGRDRNVED 371

Query: 376 ADITKLVYLQAIVKETMRLSPAGPLLGPREFYKDCVVAGYFIPKGTQLIPNIWKIQTDPR 435
           +DI  LVY+QAI+KET+RL PAGPLLG RE  +DC VAGY + +GT+++ N+WKIQ DPR
Sbjct: 372 SDIENLVYIQAIIKETLRLYPAGPLLGHREAIEDCTVAGYNVRRGTRMLVNVWKIQRDPR 431

Query: 436 VWPDPFEFKPERFLTTH-KDVDLKGNNFELIPFGSGRRGCPAIAFGLQMVHFTLAGFLQS 495
           V+ +P EF+PERF+T   K+ D++G NFEL+PFGSGRR CP  +  +Q++H  LA FLQS
Sbjct: 432 VYMEPNEFRPERFITGEAKEFDVRGQNFELMPFGSGRRSCPGSSLAMQVLHLGLARFLQS 491

Query: 496 FDIKNPTSEPVDMTENFGMANEKVVPLDVLVTPRL 524
           FD+K     PVDMTE+ G+   K  PL++L++PRL
Sbjct: 492 FDVKTVMDMPVDMTESPGLTIPKATPLEILISPRL 517

BLAST of Tan0005335 vs. TAIR 10
Match: AT4G31950.1 (cytochrome P450, family 82, subfamily C, polypeptide 3 )

HSP 1 Score: 428.3 bits (1100), Expect = 8.8e-120
Identity = 238/529 (44.99%), Postives = 323/529 (61.06%), Query Frame = 0

Query: 7   SLNPLSVATLIIVLFVSCYFLWKKIGGVCEPK--KAPSPPGAWPIIGHLHLL-NKGKIPH 66
           SL  L V+ L+ V F++ +   KK      PK  KAP+P GAWPIIGHLHLL  K ++ +
Sbjct: 4   SLFSLFVSILVFV-FIALFKKSKK------PKYVKAPAPSGAWPIIGHLHLLGGKEQLLY 63

Query: 67  HVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKESLCTNDNAAASRPELSVSKHFSYDFA 126
             LG MAD YGP   L+LGS    V S++E+AK+    ND A AS    + +KH  Y F 
Sbjct: 64  RTLGKMADHYGPAMSLRLGSSETFVGSSFEVAKDCFTVNDKALASL-MTAAAKHMGYVF- 123

Query: 127 MFGLASYSPYWRDMRKIANLELLSNPRVDQVKNIMFGEIDTSLTELHERWAVEKNESGQV 186
                     W +MRKIA +ELLSN R+  + N+   EI   + +L+  W V+K  S  V
Sbjct: 124 ----------WLEMRKIAMIELLSNRRLQMLNNVRVSEISMGVKDLYSLW-VKKGGSEPV 183

Query: 187 PVEMKRWFGDAIVNMLLKTIIGKRCVGPNAMGGE---KEAKELQMAIRDSFHLMGEGLLR 246
            V++K W  D I NM+++ + GKR  G          +EA++ +  I   FHL+G   + 
Sbjct: 184 MVDLKSWLEDMIANMIMRMVAGKRYFGGGGAESSEHTEEARQWRKGIAKFFHLVGIFTVS 243

Query: 247 DYIPVIGKLGFDGNVKAMEKIAKRIDAIMAGWLEEHKRNRANGNLENKERDVNFMDSLLS 306
           D  P +G L   G+ K M++  + +D I+  W+E H++ R       K  D +F+D +LS
Sbjct: 244 DAFPKLGWLDLQGHEKEMKQTRRELDVILERWIENHRQQRKVSG--TKHNDSDFVDVMLS 303

Query: 307 LYDAKKLPR-YYDGDTIIKATTLTMISGGTESSTVTLTWAMSLLLNNPHTLRRAYQELDE 366
           L +  KL    YD +T IK T L +I GG+E+S  TLTWA+SLLLNN   L++   E+D 
Sbjct: 304 LAEQGKLSHLQYDANTCIKTTCLALILGGSETSPSTLTWAISLLLNNKDMLKKVQDEIDI 363

Query: 367 VVGRDRRVNEADITKLVYLQAIVKETMRLSPAGPLLGPREFYKDCVVAGYFIPKGTQLIP 426
            VGRDR V ++DI  LVYLQAI+KET+RL PA PLLG RE  +DC VAGY +P GT+LI 
Sbjct: 364 HVGRDRNVEDSDIKNLVYLQAIIKETLRLYPAAPLLGHREAMEDCTVAGYNVPCGTRLIV 423

Query: 427 NIWKIQTDPRVWPDPFEFKPERFLTTH-KDVDLKGNNFELIPFGSGRRGCPAIAFGLQMV 486
           N+WKIQ DP+V+ +P EF+PERF+T   KD D++G NFEL+PFGSGRR CP  +  +QM+
Sbjct: 424 NVWKIQRDPKVYMEPNEFRPERFITGEAKDFDVRGQNFELMPFGSGRRSCPGPSLAMQML 483

Query: 487 HFTLAGFLQSFDIKNPTSEPVDMTENFGMANEKVVPLDVLVTPRLPLHL 528
           H  LA FL SF++K     PVDM+E+ G+   K  PL+VL+ PRL   L
Sbjct: 484 HLGLARFLHSFEVKTVLDRPVDMSESPGLTITKATPLEVLINPRLKREL 510

BLAST of Tan0005335 vs. TAIR 10
Match: AT2G25160.1 (cytochrome P450, family 82, subfamily F, polypeptide 1 )

HSP 1 Score: 390.2 bits (1001), Expect = 2.7e-108
Identity = 215/525 (40.95%), Postives = 303/525 (57.71%), Query Frame = 0

Query: 16  LIIVLFVSCYFLWK---KIGGVCEPKK----APSPPGAWPIIGHLHLLNKGKIPHHVLGA 75
           LI++  +S  F++     I     PK     AP  PGAWP++GHLHL +     H   GA
Sbjct: 3   LIMLFLLSALFIFPVLILIKSRLRPKNKKSTAPMVPGAWPLLGHLHLFDTVNPTHVTFGA 62

Query: 76  MADKYGPIFRLQLGSRSALVVSNWEMAKESLCTNDNAAASRPELSVSKHFSYDFAMFGLA 135
           MAD YGP+F  +LGS   +++++ E+AKE    +D     RPEL+ SK   Y+ +    +
Sbjct: 63  MADVYGPVFMAKLGSIKVMIINSKEVAKEIYTVHDK-LLERPELTASKLLGYNDSFLTFS 122

Query: 136 SYSPYWRDMRKIANLELLSNPRVDQVKNIMFGEIDTSLTELHERWAVEKNESGQVPVEMK 195
            Y  YWR++RKIA  EL S   VD        E D +   L+ RW         V V+MK
Sbjct: 123 PYGLYWREIRKIAVSELFSTSGVDMHMVSRAREADLAFRALYVRWEKRGKPKEGVLVDMK 182

Query: 196 RWFGDAIVNMLLKTIIGKRCVGPNAMGGEKEAKELQMAIRDSFHLMGEGLLRDYIPVIGK 255
           + F D   N+ L  + GKR  G N     KEA+     IR+        LL D  PV+G 
Sbjct: 183 QEFIDLTANISLMMVSGKRYFGENPNCEVKEARRCGKLIREFLDYFALFLLSDVAPVLGF 242

Query: 256 LGFDGNVKAMEKIAKRIDAIMAGWLEEHKRNRA-NGNLENKERDVNFMDSLLSLYDAKKL 315
           L +    + M++ AK +D +  GW+EEHK  R+ +G  EN     +++D L+ +    K+
Sbjct: 243 LDWK-TKRGMKRTAKGLDKVAEGWIEEHKNKRSDHGRSEN-----DYLDILIKILGQDKI 302

Query: 316 PRYYDGDTIIKATTLTMISGGTESSTVTLTWAMSLLLNNPHTLRRAYQELDEVVGRDRRV 375
           P   D  T IKA  L ++  G+E++ V L WA+SLLLNNPH LR+A +ELD  +G++R V
Sbjct: 303 PGLSDTHTKIKALCLNLVLAGSETAIVVLVWAVSLLLNNPHVLRKAQEELDSKIGKERVV 362

Query: 376 NEADITKLVYLQAIVKETMRLSPAGPLLGPREFYKDCVVA--GYFIPKGTQLIPNIWKIQ 435
            E DI  LVYLQAIVKET RL P  PL+  R   +D  +A     +P GTQL+ + WKI 
Sbjct: 363 EELDIKDLVYLQAIVKETFRLYPPVPLVAYRAVVEDFDIAFCKCHVPAGTQLMVSAWKIH 422

Query: 436 TDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPAIAFGLQMVHFTLAGF 495
            DP VW +P +F+PERFLT+++++D+ G +++  PFG GRR CPAI  G++MVH+ L  F
Sbjct: 423 RDPNVWSNPEQFEPERFLTSNRELDVGGQSYKFFPFGLGRRSCPAIPLGMRMVHYLLVRF 482

Query: 496 LQSFDIKNPTSEPVDMTENFGMANEKVVPLDVLVTPRLPLHLHGI 531
           L SFD+  P+S+ VDMTE+ G+ N K  PL+V + PRL   L+ +
Sbjct: 483 LHSFDLARPSSQDVDMTESNGLVNHKATPLEVNIIPRLHKSLYEV 520

BLAST of Tan0005335 vs. TAIR 10
Match: AT3G25180.1 (cytochrome P450, family 82, subfamily G, polypeptide 1 )

HSP 1 Score: 368.2 bits (944), Expect = 1.1e-101
Identity = 207/529 (39.13%), Postives = 304/529 (57.47%), Query Frame = 0

Query: 5   LASLNPLSVATLIIVLFVSCYFLWKKIGGVCEPKKA--PSPPGAWPIIGHLHLLNKGKIP 64
           L S   LS+ +L +V+F   Y   +K    CE   +  P P GA P+ GHLHLL   K+ 
Sbjct: 4   LFSTLQLSLFSLALVIF--GYIFLRKQLSRCEVDSSTIPEPLGALPLFGHLHLLRGKKLL 63

Query: 65  HHVLGAMADKYGPIFRLQLGSRSALVVSNWEMAKESLCTNDNAAASRPELSVSKHFSYDF 124
              L AM+ K+GPIF L+LG    +V S+ +  K+   TND A A+RP ++  ++  Y+ 
Sbjct: 64  CKKLAAMSQKHGPIFSLKLGFYRLVVASDPKTVKDCFTTNDLATATRPNIAFGRYVGYNN 123

Query: 125 AMFGLASYSPYWRDMRKIANLELLSNPRVDQVKNIMFGEIDTSLTELHERWAVEKNESGQ 184
           A   LA Y  YWR++RKI  + L SN  ++ + +I   E++T +  L+      K   G 
Sbjct: 124 ASLTLAPYGDYWRELRKIVTVHLFSNHSIEMLGHIRSSEVNTLIKHLY------KGNGGT 183

Query: 185 VPVEMKRWFGDAIVNMLLKTIIGKRCVGPNAMGGEKEAKELQMAIRDSFHLMGEGLLRDY 244
             V++   F     N++L+ ++GKR         E   KE   A++   +L    ++ D 
Sbjct: 184 SIVKIDMLFEFLTFNIILRKMVGKRIGFGEVNSDEWRYKE---ALKHCEYLAVIPMIGDV 243

Query: 245 IPVIGKLGFDGNVKAMEKIAKRIDAIMAGWLEEHKRNRANGNLENKERDVNFMDSLLSLY 304
           IP +G L F  N   M+++ K +D++   WL EH + R+      K+++   MD LL + 
Sbjct: 244 IPWLGWLDFAKN-SQMKRLFKELDSVNTKWLHEHLKKRSR---NEKDQERTIMDLLLDIL 303

Query: 305 DAKKLPRYYDGDTIIKATTLTMISGGTESSTVTLTWAMSLLLNNPHTLRRAYQELDEVVG 364
               +   +  D I+KAT L +   G++S+++TLTWA+SLLLNNP  L  A +E+D  VG
Sbjct: 304 PEDIVISGHVRDVIVKATILALTLTGSDSTSITLTWAVSLLLNNPAALEAAQEEIDNSVG 363

Query: 365 RDRRVNEADITKLVYLQAIVKETMRLSPAGPLLGPREFYKDCVVAGYFIPKGTQLIPNIW 424
           + R + E+DI  L YLQAIVKET RL P  PL G RE  +DC V GY + KGT+L+ NIW
Sbjct: 364 KGRWIEESDIQNLKYLQAIVKETHRLYPPAPLTGIREAREDCFVGGYRVEKGTRLLVNIW 423

Query: 425 KIQTDPRVWPDPFEFKPERFLTTHKDVDLKGNNFELIPFGSGRRGCPAIAFGLQMVHFTL 484
           K+  DP++WPDP  FKPERF+      +   +NFE IPFGSGRR CP +  GL++VHF L
Sbjct: 424 KLHRDPKIWPDPKTFKPERFMEDKSQCE--KSNFEYIPFGSGRRSCPGVNLGLRVVHFVL 483

Query: 485 AGFLQSFDIKNPTSEPVDMTENFGMANEKVVPLDVLVTPRLPLHLHGIV 532
           A  LQ F++   + EP+DM E  G+A  K+ P++V+V PRL   L+ ++
Sbjct: 484 ARLLQGFELHKVSDEPLDMAEGPGLALPKINPVEVVVMPRLDPKLYSLL 515

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O498586.8e-14950.48Cytochrome P450 82A3 OS=Glycine max OX=3847 GN=CYP82A3 PE=2 SV=1[more]
O498598.6e-14449.24Cytochrome P450 82A4 OS=Glycine max OX=3847 GN=CYP82A4 PE=2 SV=1[more]
Q430681.3e-13644.86Cytochrome P450 82A1 (Fragment) OS=Pisum sativum OX=3888 GN=CYP82A1 PE=2 SV=2[more]
A0A0N7F2973.8e-13647.41Demethylepipodophyllotoxin synthase OS=Sinopodophyllum hexandrum OX=93608 GN=CYP... [more]
Q9SZ466.4e-13146.32Xanthotoxin 5-hydroxylase CYP82C4 OS=Arabidopsis thaliana OX=3702 GN=CYP82C4 PE=... [more]
Match NameE-valueIdentityDescription
XP_022976389.11.7e-24578.91cytochrome P450 82A3-like [Cucurbita maxima][more]
XP_023535454.15.0e-24277.59cytochrome P450 CYP82D47-like [Cucurbita pepo subsp. pepo][more]
XP_022935738.15.5e-24177.40cytochrome P450 82A3-like [Cucurbita moschata][more]
XP_038897047.11.2e-24074.62cytochrome P450 CYP82D47-like [Benincasa hispida][more]
XP_022936278.11.6e-24077.02cytochrome P450 CYP82D47-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1IND18.1e-24678.91cytochrome P450 82A3-like OS=Cucurbita maxima OX=3661 GN=LOC111476807 PE=3 SV=1[more]
A0A6J1FBC62.7e-24177.40cytochrome P450 82A3-like OS=Cucurbita moschata OX=3662 GN=LOC111442561 PE=3 SV=... [more]
A0A6J1D7467.8e-24177.01cytochrome P450 82A3-like OS=Momordica charantia OX=3673 GN=LOC111017857 PE=3 SV... [more]
A0A6J1FCU27.8e-24177.02cytochrome P450 CYP82D47-like OS=Cucurbita moschata OX=3662 GN=LOC111442946 PE=3... [more]
A0A0A0LD432.3e-24074.06Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G852590 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G31940.14.5e-13246.32cytochrome P450, family 82, subfamily C, polypeptide 4 [more]
AT4G31970.11.7e-13146.21cytochrome P450, family 82, subfamily C, polypeptide 2 [more]
AT4G31950.18.8e-12044.99cytochrome P450, family 82, subfamily C, polypeptide 3 [more]
AT2G25160.12.7e-10840.95cytochrome P450, family 82, subfamily F, polypeptide 1 [more]
AT3G25180.11.1e-10139.13cytochrome P450, family 82, subfamily G, polypeptide 1 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 94..115
score: 24.87
coord: 468..491
score: 22.95
coord: 336..362
score: 35.95
coord: 316..333
score: 20.82
coord: 70..89
score: 37.09
coord: 379..397
score: 36.15
coord: 420..444
score: 41.12
coord: 458..468
score: 53.79
IPR001128Cytochrome P450PRINTSPR00385P450coord: 327..344
score: 31.45
coord: 380..391
score: 39.97
coord: 459..468
score: 57.4
IPR001128Cytochrome P450PFAMPF00067p450coord: 41..508
e-value: 6.3E-90
score: 302.1
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 35..519
e-value: 6.5E-117
score: 393.0
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 41..522
NoneNo IPR availablePANTHERPTHR47947:SF18CYTOCHROME P450 82A2coord: 10..526
NoneNo IPR availablePANTHERPTHR47947CYTOCHROME P450 82C3-RELATEDcoord: 10..526
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 461..470

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0005335.1Tan0005335.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0098542 defense response to other organism
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen