Tan0005332 (gene) Snake gourd v1

BLAST of Tan0005332 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 577.8 bits (1488), Expect = 1.3e-163
Identity = 296/536 (55.22%), Postives = 375/536 (69.96%), Query Frame = 0

Query: 25  SAANDSLFCNFTSNPSYCISVLQKSTGNVFDSGRFSMRRSLSKASRFLDLIEKHL-HNSS 84
           +A + S  C F  +PSYC SVL    G+++  GR S+RRSLS+A RF+ +I+  L     
Sbjct: 31  NATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGK 90

Query: 85  TLPKSVAGALTDCQYLAQLNINFLSNSFRAINGTDKKLTFSKADYVQSLLSAILTNIDTC 144
              KS  GAL DC++LA L +++L +S +  + T K L+ S+A+ V + LSA +TN  TC
Sbjct: 91  VAAKSTVGALEDCKFLASLTMDYLLSSSQTADST-KTLSLSRAEDVHTFLSAAITNEQTC 150

Query: 145 LDGLKTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVP-RNRNRTLEHP---VKKHL 204
           L+GLK+ AS + L  D    L + TK Y +SL LF+KGWVP R R+R +  P    KK  
Sbjct: 151 LEGLKSTASENGLSGD----LFNDTKLYGVSLALFSKGWVPRRQRSRPIWQPQARFKKFF 210

Query: 205 QFRKGPLPLRMSRHDRAVYNSMVQETSLPSQKLLMQLQTILS------------------ 264
            FR G LPL+M+   RAVYN++ +   L S    +Q+  I++                  
Sbjct: 211 GFRNGKLPLKMTERARAVYNTVTRRKLLQSDADAVQVSDIVTVIQNGTGNFTTINAAIAA 270

Query: 265 ------AANGYFLIYIMAGVYQEYVSIPSKKKYLLMIGDGINQTIVTGNRSVADGWTTFN 324
                  +NGYFLIY+ AG+Y+EYV +P  K+Y++MIGDGINQT++TGNRSV DGWTTFN
Sbjct: 271 APNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFN 330

Query: 325 SATFAVAAEGFMAVNITFQNRAGAIKGQAVAVRSGADMSIFYSCSFEGYQDTLYTHSLRQ 384
           SATF ++   F+ VNIT +N AG  KGQAVA+RSG D+S+FYSCSFE YQDTLYTHSLRQ
Sbjct: 331 SATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQ 390

Query: 385 FFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPGQSNMITAQGRSDPNQNTGTSIHNCT 444
           F+RECD+YGTVDFIFGNAAVV QNCN+YPR PR GQSN +TAQGR+DPNQNTGT+IH CT
Sbjct: 391 FYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCT 450

Query: 445 IRATSDLAASNSYMNKTYLGRPWKQYSRTVYMQTFIDGFINSAGWDPWSGEY-LSTLYYG 504
           IR   DLA SN Y  KTYLGRPWK+YSRTV MQT+IDGF+  +GW+ WSG++ LSTLYY 
Sbjct: 451 IRPADDLATSN-YTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYA 510

Query: 505 EYNNTGAGSNIKNRVTWPGYHVIYNVTNAVNFTVSNLLFGDVWLPPSDVPYTGGFM 531
           EYNNTG GS+  NRVTWPGYHVI N T+A NFTV+N L G+ W+  + VP+ GG +
Sbjct: 511 EYNNTGPGSDTTNRVTWPGYHVI-NATDASNFTVTNFLVGEGWIGQTGVPFVGGLI 559

BLAST of Tan0005332 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 560.5 bits (1443), Expect = 2.1e-158
Identity = 306/567 (53.97%), Postives = 382/567 (67.37%), Query Frame = 0

Query: 10  LVASFLILLTLFSSSS----AANDSLFCNFTSNPSYCISVL-QKSTGNVFDSGRFSMRRS 69
           LV  FL L TLF +S     + + S  C  T +P +C SV  Q S G+V + GRFS+R+S
Sbjct: 8   LVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLRKS 67

Query: 70  LSKASRFLDLIEKHL-HNSSTLPKSVAGALTDCQYLAQLNINFLSNSFRAINGT--DKKL 129
           L+++ +F   I+++L  N++ L +S  GAL DC+YLA L  ++L  SF  +N T   K L
Sbjct: 68  LTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTL 127

Query: 130 TFSKADYVQSLLSAILTNIDTCLDGLKTVASGS-SLEKDLLAPLIDCTKSYSLSLDLFTK 189
           +FSKAD +Q+LLSA LTN  TCLDG+ T AS S ++   +  PLI+ TK +S+SL LFTK
Sbjct: 128 SFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFTK 187

Query: 190 GWVPRNRNRTLE----HPVKKH------LQFRKGPLPLRMSRHDRAVYNS---------- 249
           GWVP+ + +       HP   H        FR G LPL+M+ H RAVY S          
Sbjct: 188 GWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKLADDD 247

Query: 250 ------MVQETSLPSQKLLMQLQTILSAAN----------GYFLIYIMAGVYQEYVSIPS 309
                 +V +    +Q       TI  A N          GYF+IY+ +GVY+E V I  
Sbjct: 248 NDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAK 307

Query: 310 KKKYLLMIGDGINQTIVTGNRSVADGWTTFNSATFAVAAEGFMAVNITFQNRAGAIKGQA 369
            K+YL+MIGDGIN+T+VTGNR+V DGWTTFNSATFAV +  F+AVN+TF+N AG  K QA
Sbjct: 308 NKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQA 367

Query: 370 VAVRSGADMSIFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYP 429
           VA+RS AD+SIFYSCSFE YQDTLYTHSLRQF+RECDIYGTVDFIFGNAAVVFQ+CN+YP
Sbjct: 368 VAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYP 427

Query: 430 RLPRPGQSNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASNSYMNKTYLGRPWKQYSRT 489
           R P   Q N ITAQGR+DPNQNTG SIHNCTI+   DL +SN Y  KTYLGRPWK+YSRT
Sbjct: 428 RQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSN-YTVKTYLGRPWKEYSRT 487

Query: 490 VYMQTFIDGFINSAGWDPWSGEY-LSTLYYGEYNNTGAGSNIKNRVTWPGYHVIYNVTNA 531
           V+MQ++ID  +   GW  W+G++ LSTLYY EYNNTG+GS+  +RV WPGYHVI N T+A
Sbjct: 488 VFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVI-NSTDA 547

BLAST of Tan0005332 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 557.4 bits (1435), Expect = 1.8e-157
Identity = 301/576 (52.26%), Postives = 378/576 (65.62%), Query Frame = 0

Query: 6   LFPPLVASFLILLTLFSSSSAANDSLFCNFTSNPSYCISVLQKSTGNVFDSGRFSMRRSL 65
           +F  +  SF +   L SS + +N S  C  T +P YC SV   S GNV   G FS+R+SL
Sbjct: 5   IFILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSL 64

Query: 66  SKASRFLDLIEKHLHNSSTLPK-SVAGALTDCQYLAQLNINFLSNSFRAINGTD-----K 125
           S++ +F+  +++++  ++ L + +V  AL DC++LA L +++L  SF  +N T      K
Sbjct: 65  SQSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFK 124

Query: 126 KLTFSKADYVQSLLSAILTNIDTCLDGLKTVASGS---SLEKDLLAPLIDCTKSYSLSLD 185
            L+F KAD +Q+LLSA LTN  TCL+GL T AS S   ++   +  PL++ TK   +SL 
Sbjct: 125 PLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLA 184

Query: 186 LFTKGWVPRNRNRT----------LEHPVKKHLQFRKGPLPLRMSRHDRAVYNSMVQETS 245
           LFTKGWVP+ + R                K    FR G LPL+M+   +AVY S+ +   
Sbjct: 185 LFTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKL 244

Query: 246 LP----------SQKLLMQLQTI---------------------LSAANGYFLIYIMAGV 305
                       S  L+  + T+                        + G+FLIY+ AG+
Sbjct: 245 ADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGI 304

Query: 306 YQEYVSIPSKKKYLLMIGDGINQTIVTGNRSVADGWTTFNSATFAVAAEGFMAVNITFQN 365
           Y+EY+SI   K+Y++MIGDGINQT+VTGNRSV DGWTTFNSATFAV A  F+AVNITF+N
Sbjct: 305 YEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRN 364

Query: 366 RAGAIKGQAVAVRSGADMSIFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAV 425
            AG  K QAVA+RSGAD SIFYSCSFE YQDTLYTHSLRQF+RECD+YGTVDFIFGNAAV
Sbjct: 365 TAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAV 424

Query: 426 VFQNCNIYPRLPRPGQSNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASNSYMNKTYLG 485
           VFQNCN+YPR P P Q N ITAQGRSDPNQNTGTSI NCTI+   DL +SN Y  KTYLG
Sbjct: 425 VFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSN-YTVKTYLG 484

Query: 486 RPWKQYSRTVYMQTFIDGFINSAGWDPWSGEY-LSTLYYGEYNNTGAGSNIKNRVTWPGY 531
           RPWK+YSRTVYMQ++IDGF+   GW  W+G++ LSTLYY EYNNTG GSN  NRVTWPGY
Sbjct: 485 RPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGY 544

BLAST of Tan0005332 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 414.8 bits (1065), Expect = 1.4e-114
Identity = 236/548 (43.07%), Postives = 327/548 (59.67%), Query Frame = 0

Query: 24  SSAANDSLFCNFTSNPSYCISVL---QKSTGNVFDSGRFSMRRSLSKASRFLDLIEKHLH 83
           S + + SL C  T  P  C ++L   + S  + +  G+F++++ L +ASR   +I  +  
Sbjct: 74  SQSQSPSLACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYAR 133

Query: 84  NSSTLPKSVA----GALTDCQYLAQLNINFLSNSFRAINGTDKKLTFSKADYVQSLLSAI 143
              + P S      GA+ DC  L++L++N+L      +  T + +T +  ++V SLLS +
Sbjct: 134 RVESKPGSATAEEIGAVADCGELSELSVNYLETVTTELK-TAQVMTAALVEHVNSLLSGV 193

Query: 144 LTNIDTCLDGLKTVASGSSLEKDLLAPLIDCTKSYSLSLDLF-------------TKGWV 203
           +TN  TCLDGL    SG +    + +P+ + T+ YS+SL L              +KG +
Sbjct: 194 VTNQQTCLDGLVEAKSGFAAA--IGSPMGNLTRLYSISLGLVSHALNRNLKRFKASKGKI 253

Query: 204 PRNRNRTLEHPVKKHLQ-FRK---GPLPLRMSRHDRAVYNSMVQETSLPSQKLLM----- 263
               N T   P++  ++  RK        R +  +          + L S+ +++     
Sbjct: 254 LGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKS 313

Query: 264 -QLQTILSA----------ANGYFLIYIMAGVYQEYVSIPSKKKYLLMIGDGINQTIVTG 323
               TI  A           +GYF+IY   GVY+EY+ +P  KK L+++GDGIN+TI+TG
Sbjct: 314 DNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITG 373

Query: 324 NRSVADGWTTFNSATFAVAAEGFMAVNITFQNRAGAIKGQAVAVRSGADMSIFYSCSFEG 383
           N +V DGWTT+N ++FAV  E FMAV++TF+N AG  K QAVA+R+ A+ S FY CSFEG
Sbjct: 374 NHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEG 433

Query: 384 YQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPGQSNMITAQGRSDP 443
           YQDTLY HSLRQF+RECDIYGTVDFIFGNAA +FQNCNIY R P   Q N ITA GR DP
Sbjct: 434 YQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDP 493

Query: 444 NQNTGTSIHNCTIRATSDLAASNSYMNKTYLGRPWKQYSRTVYMQTFIDGFINSAGWDPW 503
           NQNTG SI NCTI+A  DLAA       T+LGRPWK YSRTV+MQ++I   +   GW  W
Sbjct: 494 NQNTGISIINCTIKAAPDLAAEPK-SAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEW 553

Query: 504 SGEY-LSTLYYGEYNNTGAGSNIKNRVTWPGYHVIYNVTNAVNFTVSNLLFGDVWLPPSD 531
           +G   L T+YYGEY+N G G+N   RV W GY+++ N+  A+NFTV N   GD WLP +D
Sbjct: 554 NGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLL-NLAEAMNFTVYNFTMGDTWLPQTD 613

BLAST of Tan0005332 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 397.9 bits (1021), Expect = 1.8e-109
Identity = 229/550 (41.64%), Postives = 319/550 (58.00%), Query Frame = 0

Query: 22  SSSSAANDSLFCNFTSNPSYC---ISVLQKSTGNVFDSGRFSMRRSLSKASRFLDLIEKH 81
           S  S    ++ C  T  P  C   +S ++ S  + +  G+F+M++ L +A R   +I + 
Sbjct: 77  SQPSPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRF 136

Query: 82  LH----NSSTLPKSVAGALTDCQYLAQLNINFLSNSFRAINGTDKKLTFSKADYVQSLLS 141
                 +  T       A+ DC  LA+L++ +L      +   +  +T +  D V SLL 
Sbjct: 137 AQRVEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAE-LMTAALVDRVTSLLG 196

Query: 142 AILTNIDTCLDGLKTVASGSSLEKDLLAPLIDCTKSYSLSLDLF-------------TKG 201
            ++TN  TCLDGL    SG +    +  PL + T+ YS+SL L              +KG
Sbjct: 197 GVVTNQQTCLDGLVDAKSGFATA--IGTPLGNLTRLYSVSLGLVSHALNRNLKRYKGSKG 256

Query: 202 WVPRNRNRTLEHPVKKHLQF-----RKGPLPLRMSRH-----DRAVYNSMVQETSLPSQK 261
            +    N+ +  P++  ++       KG    + +R+     + +  + +V+E       
Sbjct: 257 KIFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPY 316

Query: 262 LLMQLQTILSAA----------NGYFLIYIMAGVYQEYVSIPSKKKYLLMIGDGINQTIV 321
                 TI  A            GYF+IY  AG+Y+EYV I +KK+ +++IGDGIN+TI+
Sbjct: 317 ETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTII 376

Query: 322 TGNRSVADGWTTFNSATFAVAAEGFMAVNITFQNRAGAIKGQAVAVRSGADMSIFYSCSF 381
           +GN S  DGWTT+NS+TFAV  + F+AV++TF+N AG  K QAVAVR+ AD S FY CSF
Sbjct: 377 SGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSF 436

Query: 382 EGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPGQSNMITAQGRS 441
           EGYQDTLY HSLRQF+RECDIYGT+DFIFGNAA +FQNCNIY R P   Q N +TA GR+
Sbjct: 437 EGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRT 496

Query: 442 DPNQNTGTSIHNCTIRATSDLAASNSYMNKTYLGRPWKQYSRTVYMQTFIDGFINSAGWD 501
           DPNQ TG SI NCTI A  DLAA +     T+LGRPWK YSRTVY+Q++I   +   GW 
Sbjct: 497 DPNQKTGISIINCTIGAAPDLAA-DPKSTMTFLGRPWKPYSRTVYIQSYISDVVQPVGWL 556

Query: 502 PWSGEY-LSTLYYGEYNNTGAGSNIKNRVTWPGYHVIYNVTNAVNFTVSNLLFGDVWLPP 531
            W+G   L T+ YGEY+N G G++   RV W GY ++ N+  A+NFTV N   GD WLP 
Sbjct: 557 EWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLL-NLVQAMNFTVYNFTLGDTWLPQ 616

BLAST of Tan0005332 vs. NCBI nr
Match: XP_038898430.1 (probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Benincasa hispida] >XP_038898431.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Benincasa hispida] >XP_038898432.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Benincasa hispida])

HSP 1 Score: 905.6 bits (2339), Expect = 2.0e-259
Identity = 460/565 (81.42%), Postives = 490/565 (86.73%), Query Frame = 0

Query: 1   MACKFLFPPLVASFLILLTLFSSSSAANDSL--------FCNFTSNPSYCISVLQKST-G 60
           MACKFLFPPL+ASFLILLTLF+SSSA  DSL         C FTSNPSYCI+VL+KST G
Sbjct: 1   MACKFLFPPLLASFLILLTLFTSSSAEYDSLTNFPPSKAICKFTSNPSYCITVLKKSTGG 60

Query: 61  NVFDSGRFSMRRSLSKASRFLDLIEKHLHNSSTLPKSVAGALTDCQYLAQLNINFLSNSF 120
           N++DSGRFS+RRSLSKASRF DLIEKHL NSSTLPKS+ GALTDCQYLAQLNINFLSNSF
Sbjct: 61  NIYDSGRFSIRRSLSKASRFFDLIEKHLQNSSTLPKSITGALTDCQYLAQLNINFLSNSF 120

Query: 121 RAINGTDKKLTFSKADYVQSLLSAILTNIDTCLDGLKTVASGSSLEKDLLAPLIDCTKSY 180
           RA+NGTDKKLT+SKADY+QSLLSAILTNIDTCLDGLKTVA+GSSLEKDLLAPLIDCTKSY
Sbjct: 121 RAVNGTDKKLTYSKADYIQSLLSAILTNIDTCLDGLKTVAAGSSLEKDLLAPLIDCTKSY 180

Query: 181 SLSLDLFTKGWVP-RNRNRTLEHPVKKHLQFRKGPLPLRMSRHDRAVYNSMVQETSLPSQ 240
           SLSLDLFTKGWVP RNRNRTL+HP KKHLQFRKGPLPLRMSR DRAVY+S+     L S 
Sbjct: 181 SLSLDLFTKGWVPRRNRNRTLDHPGKKHLQFRKGPLPLRMSRRDRAVYDSVANRRKLSSS 240

Query: 241 K---LLMQLQTILS----------------------AANGYFLIYIMAGVYQEYVSIPSK 300
               +L+    ++S                      AANGYFLIYI AGVYQEYVSIPSK
Sbjct: 241 SDDGVLVNGIAVVSQDGKGDFLNITDAINAAPNNSLAANGYFLIYISAGVYQEYVSIPSK 300

Query: 301 KKYLLMIGDGINQTIVTGNRSVADGWTTFNSATFAVAAEGFMAVNITFQNRAGAIKGQAV 360
           KKYLLMIGDGINQTI+TGN SV DGWTTFNSATFAVAAEGFMAVNITFQN AGAIKGQAV
Sbjct: 301 KKYLLMIGDGINQTIITGNHSVVDGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAV 360

Query: 361 AVRSGADMSIFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420
           A+RSGADM +FYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR
Sbjct: 361 ALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420

Query: 421 LPRPGQSNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASNSYMNKTYLGRPWKQYSRTV 480
           LPRPGQ+NMITAQGRSDPNQNTGTSIHNC IRATSDLAAS+SYMNKTYLGRPWKQYSRTV
Sbjct: 421 LPRPGQANMITAQGRSDPNQNTGTSIHNCAIRATSDLAASSSYMNKTYLGRPWKQYSRTV 480

Query: 481 YMQTFIDGFINSAGWDPWSGEYLSTLYYGEYNNTGAGSNIKNRVTWPGYHVIYNVTNAVN 531
           YMQTFIDGF+N +GWDPW+GEYLSTLYYGEYNNTG GSN KNRV W GYHVI N T+A N
Sbjct: 481 YMQTFIDGFVNPSGWDPWTGEYLSTLYYGEYNNTGDGSNTKNRVNWAGYHVINNATDAAN 540

BLAST of Tan0005332 vs. NCBI nr
Match: XP_031738568.1 (probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis sativus] >XP_031738569.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis sativus] >XP_031738570.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis sativus] >KGN60252.1 hypothetical protein Csa_001115 [Cucumis sativus])

HSP 1 Score: 895.2 bits (2312), Expect = 2.7e-256
Identity = 451/565 (79.82%), Postives = 490/565 (86.73%), Query Frame = 0

Query: 1   MACKFLFPPLVASFLILLTLFSSSSAANDSL--------FCNFTSNPSYCISVLQKS-TG 60
           MACKFLFPPL+ASFLILLTLF+SS A++DSL         C  TSNPSYCI+VL++S  G
Sbjct: 1   MACKFLFPPLLASFLILLTLFTSSFASDDSLTNFFPTKAICKLTSNPSYCITVLKQSRDG 60

Query: 61  NVFDSGRFSMRRSLSKASRFLDLIEKHLHNSSTLPKSVAGALTDCQYLAQLNINFLSNSF 120
           N++DSGRFS+RRSLSKA+RFLDLIEKHL NSSTLP S+ GAL DCQYLAQLN+NFLSNSF
Sbjct: 61  NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSF 120

Query: 121 RAINGTDKKLTFSKADYVQSLLSAILTNIDTCLDGLKTVASGSSLEKDLLAPLIDCTKSY 180
           RA+NGTD+KLT+SKADY+QSLLSAILTNIDTCLDGL TVASGSSLEKDLLAPLIDCTKSY
Sbjct: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180

Query: 181 SLSLDLFTKGWVP-RNRNRTLEHPVKKHLQFRKGPLPLRMSRHDRAVYNSMVQETSLPSQ 240
           SLSLDLFTKGWVP RNRNRTLEHP KKHLQFRKGPLPLRMSRHDRAVYNS+     L S 
Sbjct: 181 SLSLDLFTKGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSS 240

Query: 241 K---LLMQLQTILS----------------------AANGYFLIYIMAGVYQEYVSIPSK 300
               +L+    ++S                      A++GYFLIYI AGVYQEYVS+PSK
Sbjct: 241 SDDGVLVNGVVVVSQDGQGDFLNITDAINAAPNNSLASDGYFLIYITAGVYQEYVSVPSK 300

Query: 301 KKYLLMIGDGINQTIVTGNRSVADGWTTFNSATFAVAAEGFMAVNITFQNRAGAIKGQAV 360
           KKYLLMIGDGINQTI+TGNRSVADGWTTFNSATFAVAAEGFMAVNIT QN AGAIKGQAV
Sbjct: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAV 360

Query: 361 AVRSGADMSIFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420
           A+RSGADM +FYSCSFEG+QDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR
Sbjct: 361 ALRSGADMCVFYSCSFEGFQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420

Query: 421 LPRPGQSNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASNSYMNKTYLGRPWKQYSRTV 480
           LPRPGQ+NMITAQGRSDPNQNTGTSIHNCTIRAT +LAAS+SYMNKTYLGRPWKQYSRTV
Sbjct: 421 LPRPGQANMITAQGRSDPNQNTGTSIHNCTIRATPELAASSSYMNKTYLGRPWKQYSRTV 480

Query: 481 YMQTFIDGFINSAGWDPWSGEYLSTLYYGEYNNTGAGSNIKNRVTWPGYHVIYNVTNAVN 531
           YMQTFIDGF+N  GWDPW+GEYLSTLYYGEYNNTG GS+ KNRVTW GYHVI NVT+A N
Sbjct: 481 YMQTFIDGFVNPKGWDPWTGEYLSTLYYGEYNNTGGGSDTKNRVTWAGYHVINNVTDAAN 540

BLAST of Tan0005332 vs. NCBI nr
Match: XP_016903565.1 (PREDICTED: probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis melo] >KAA0038671.1 putative pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis melo var. makuwa] >TYK31280.1 putative pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 893.6 bits (2308), Expect = 8.0e-256
Identity = 454/565 (80.35%), Postives = 488/565 (86.37%), Query Frame = 0

Query: 1   MACKFLFPPLVASFLILLTLFSSSSAANDSL--------FCNFTSNPSYCISVLQKS-TG 60
           MACKFLFPPL   FLILLTLF+SS A++DSL         C  TSNPSYCI+VL++S  G
Sbjct: 1   MACKFLFPPL---FLILLTLFTSSFASDDSLTNIFPTKAICKLTSNPSYCITVLKQSRDG 60

Query: 61  NVFDSGRFSMRRSLSKASRFLDLIEKHLHNSSTLPKSVAGALTDCQYLAQLNINFLSNSF 120
           N++DSGRFS+RRSLSKASRFLDLIEKHL NSSTLPKS+ GAL DCQYLAQLN+NFLSNSF
Sbjct: 61  NIYDSGRFSIRRSLSKASRFLDLIEKHLQNSSTLPKSIIGALKDCQYLAQLNMNFLSNSF 120

Query: 121 RAINGTDKKLTFSKADYVQSLLSAILTNIDTCLDGLKTVASGSSLEKDLLAPLIDCTKSY 180
           RA+NGTD+KLT+SKADY+QSLLSAILTNIDTCLDGL TVASGSSLEKDLLAPLIDCTKSY
Sbjct: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180

Query: 181 SLSLDLFTKGWVPR-NRNRTLEHPVKKHLQFRKGPLPLRMSRHDRAVYNSMVQETSLPSQ 240
           SLSLDLFTKGWVPR NRNRTLEHP KKHLQFRKGPLPLRMSRHDRAVYNS+     L S 
Sbjct: 181 SLSLDLFTKGWVPRWNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSS 240

Query: 241 K---LLMQLQTILS----------------------AANGYFLIYIMAGVYQEYVSIPSK 300
               +L+    ++S                      AANGYFLIYI AGVYQEYVS+PSK
Sbjct: 241 SDDGVLVNGIAVVSQDGEGDFLNITDAINAAPNNSLAANGYFLIYITAGVYQEYVSVPSK 300

Query: 301 KKYLLMIGDGINQTIVTGNRSVADGWTTFNSATFAVAAEGFMAVNITFQNRAGAIKGQAV 360
           KKYLLMIGDGINQTI+TGNRSVADGWTTFNSATFAVAAEGFMAVNITFQN AGAIKGQAV
Sbjct: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAV 360

Query: 361 AVRSGADMSIFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420
           A+RSGADM +FYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR
Sbjct: 361 ALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420

Query: 421 LPRPGQSNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASNSYMNKTYLGRPWKQYSRTV 480
           LP P Q+NMITAQGRSDPNQNTGTSIHNCTI+AT DLAAS+S+MNKTYLGRPWKQYSRTV
Sbjct: 421 LPGPRQANMITAQGRSDPNQNTGTSIHNCTIQATPDLAASSSHMNKTYLGRPWKQYSRTV 480

Query: 481 YMQTFIDGFINSAGWDPWSGEYLSTLYYGEYNNTGAGSNIKNRVTWPGYHVIYNVTNAVN 531
           YMQTFIDGF+N  GWDPWSGEYLSTLYYGEYNNTG GS+ KNRV WPGYHVI N T+A N
Sbjct: 481 YMQTFIDGFVNPKGWDPWSGEYLSTLYYGEYNNTGGGSDTKNRVIWPGYHVINNATDAAN 540

BLAST of Tan0005332 vs. NCBI nr
Match: XP_023524144.1 (probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023524145.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023524146.1 probable pectinesterase/pectinesterase inhibitor 20 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 881.7 bits (2277), Expect = 3.1e-252
Identity = 439/523 (83.94%), Postives = 461/523 (88.15%), Query Frame = 0

Query: 33  CNFTSNPSYCISVLQKSTGNVFDSGRFSMRRSLSKASRFLDLIEKHLHNSSTLPKSVAGA 92
           C FTSNPSYCISVLQKS+GNV++SGRFSMRRSLSKAS FL+LIEKHL NSSTLPKSV G 
Sbjct: 21  CKFTSNPSYCISVLQKSSGNVYESGRFSMRRSLSKASAFLNLIEKHLQNSSTLPKSVTGG 80

Query: 93  LTDCQYLAQLNINFLSNSFRAINGTDKKLTFSKADYVQSLLSAILTNIDTCLDGLKTVAS 152
           L DCQYLAQLNINFLSNSFRA+NGTDKKLT+SKADY+QSLLSAILTNIDTCLDGL TVAS
Sbjct: 81  LKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTNIDTCLDGLNTVAS 140

Query: 153 GSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTLEHPVKKHLQFRKGPLPLRMSR 212
           GSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRT +HPV KHL+FRKGPLPLRMS+
Sbjct: 141 GSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRDHPVTKHLRFRKGPLPLRMSK 200

Query: 213 HDRAVYNSMVQETSLPSQK---LLMQLQTILS----------------------AANGYF 272
           HDR VYNS+     L S     +L+    ++S                      A NGYF
Sbjct: 201 HDRVVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFLTITEAVNAAPNNSLAVNGYF 260

Query: 273 LIYIMAGVYQEYVSIPSKKKYLLMIGDGINQTIVTGNRSVADGWTTFNSATFAVAAEGFM 332
           LIYI AGVYQEYVSIPSKKKYLLMIGDGINQTI+TGN+SVADGWTTFNSATFAVAAEGFM
Sbjct: 261 LIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSVADGWTTFNSATFAVAAEGFM 320

Query: 333 AVNITFQNRAGAIKGQAVAVRSGADMSIFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVD 392
           AVNITFQN AGAIKGQAVA+RSGADMSIFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVD
Sbjct: 321 AVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVD 380

Query: 393 FIFGNAAVVFQNCNIYPRLPRPGQSNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASNS 452
           FIFGNAAVVFQNCNIYPRLPR GQ+NMITAQGRSDPNQNTGTSIHNCTIRATSDLAAS S
Sbjct: 381 FIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASKS 440

Query: 453 YMNKTYLGRPWKQYSRTVYMQTFIDGFINSAGWDPWSGEYLSTLYYGEYNNTGAGSNIKN 512
           YMNKTYLGRPWKQYSRTVYMQTFIDGFIN AGWDPWSGEYLSTLYY EYNNTGAGSN KN
Sbjct: 441 YMNKTYLGRPWKQYSRTVYMQTFIDGFINPAGWDPWSGEYLSTLYYAEYNNTGAGSNTKN 500

Query: 513 RVTWPGYHVIYNVTNAVNFTVSNLLFGDVWLPPSDVPYTGGFM 531
           RVTWPGYHVI N T+A NFTVSN L GD WLPP+ VPYTGGF+
Sbjct: 501 RVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYTGGFI 543

BLAST of Tan0005332 vs. NCBI nr
Match: KAG6608276.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 880.6 bits (2274), Expect = 7.0e-252
Identity = 438/523 (83.75%), Postives = 462/523 (88.34%), Query Frame = 0

Query: 33  CNFTSNPSYCISVLQKSTGNVFDSGRFSMRRSLSKASRFLDLIEKHLHNSSTLPKSVAGA 92
           C FTSNPSYCISVLQKS+GNV++SGRFSMRRSLSKA+ FL+LIEKHL NSSTLPKSV G 
Sbjct: 21  CKFTSNPSYCISVLQKSSGNVYESGRFSMRRSLSKATTFLNLIEKHLQNSSTLPKSVTGG 80

Query: 93  LTDCQYLAQLNINFLSNSFRAINGTDKKLTFSKADYVQSLLSAILTNIDTCLDGLKTVAS 152
           L DCQYLAQLNINFLSNSFRA+NGTDKKLT+SKADY+QSLLSAILTNIDTCLDGL TVAS
Sbjct: 81  LKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTNIDTCLDGLNTVAS 140

Query: 153 GSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTLEHPVKKHLQFRKGPLPLRMSR 212
           GSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRT +HPV KHL+FRKGPLPLRMS+
Sbjct: 141 GSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRDHPVTKHLRFRKGPLPLRMSK 200

Query: 213 HDRAVYNSMVQETSLPSQK---LLMQLQTILS----------------------AANGYF 272
           HDRAVYNS+     L S     +L+    ++S                      A NGYF
Sbjct: 201 HDRAVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFLTITEAVNAAPNNSLAVNGYF 260

Query: 273 LIYIMAGVYQEYVSIPSKKKYLLMIGDGINQTIVTGNRSVADGWTTFNSATFAVAAEGFM 332
           LIYI AGVYQEYVSIPSKKKYLLMIGDGINQTI+TGN+SVADGWTTFNSATFAVAAEGFM
Sbjct: 261 LIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSVADGWTTFNSATFAVAAEGFM 320

Query: 333 AVNITFQNRAGAIKGQAVAVRSGADMSIFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVD 392
           AVNITFQN AGAIKGQAVA+RSGADMSIFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVD
Sbjct: 321 AVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVD 380

Query: 393 FIFGNAAVVFQNCNIYPRLPRPGQSNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASNS 452
           FIFGNAAVVFQNCNIYPRLPR GQ+NMITAQGRSDPNQNTGTSIHNCTIRATSDLAAS S
Sbjct: 381 FIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASKS 440

Query: 453 YMNKTYLGRPWKQYSRTVYMQTFIDGFINSAGWDPWSGEYLSTLYYGEYNNTGAGSNIKN 512
           YMNKTYLGRPWKQYSRTVYMQTFIDGFIN AGWDPWSGEYLSTLYY EYNN+GAGSN KN
Sbjct: 441 YMNKTYLGRPWKQYSRTVYMQTFIDGFINPAGWDPWSGEYLSTLYYAEYNNSGAGSNTKN 500

Query: 513 RVTWPGYHVIYNVTNAVNFTVSNLLFGDVWLPPSDVPYTGGFM 531
           RVTWPGYHVI N T+A NFTVSN L GD WLPP+ VPYTGGF+
Sbjct: 501 RVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYTGGFI 543

BLAST of Tan0005332 vs. ExPASy TrEMBL
Match: A0A0A0LHM0 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G890080 PE=3 SV=1)

HSP 1 Score: 895.2 bits (2312), Expect = 1.3e-256
Identity = 451/565 (79.82%), Postives = 490/565 (86.73%), Query Frame = 0

Query: 1   MACKFLFPPLVASFLILLTLFSSSSAANDSL--------FCNFTSNPSYCISVLQKS-TG 60
           MACKFLFPPL+ASFLILLTLF+SS A++DSL         C  TSNPSYCI+VL++S  G
Sbjct: 1   MACKFLFPPLLASFLILLTLFTSSFASDDSLTNFFPTKAICKLTSNPSYCITVLKQSRDG 60

Query: 61  NVFDSGRFSMRRSLSKASRFLDLIEKHLHNSSTLPKSVAGALTDCQYLAQLNINFLSNSF 120
           N++DSGRFS+RRSLSKA+RFLDLIEKHL NSSTLP S+ GAL DCQYLAQLN+NFLSNSF
Sbjct: 61  NIYDSGRFSIRRSLSKATRFLDLIEKHLQNSSTLPNSIIGALKDCQYLAQLNMNFLSNSF 120

Query: 121 RAINGTDKKLTFSKADYVQSLLSAILTNIDTCLDGLKTVASGSSLEKDLLAPLIDCTKSY 180
           RA+NGTD+KLT+SKADY+QSLLSAILTNIDTCLDGL TVASGSSLEKDLLAPLIDCTKSY
Sbjct: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180

Query: 181 SLSLDLFTKGWVP-RNRNRTLEHPVKKHLQFRKGPLPLRMSRHDRAVYNSMVQETSLPSQ 240
           SLSLDLFTKGWVP RNRNRTLEHP KKHLQFRKGPLPLRMSRHDRAVYNS+     L S 
Sbjct: 181 SLSLDLFTKGWVPRRNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSS 240

Query: 241 K---LLMQLQTILS----------------------AANGYFLIYIMAGVYQEYVSIPSK 300
               +L+    ++S                      A++GYFLIYI AGVYQEYVS+PSK
Sbjct: 241 SDDGVLVNGVVVVSQDGQGDFLNITDAINAAPNNSLASDGYFLIYITAGVYQEYVSVPSK 300

Query: 301 KKYLLMIGDGINQTIVTGNRSVADGWTTFNSATFAVAAEGFMAVNITFQNRAGAIKGQAV 360
           KKYLLMIGDGINQTI+TGNRSVADGWTTFNSATFAVAAEGFMAVNIT QN AGAIKGQAV
Sbjct: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITIQNTAGAIKGQAV 360

Query: 361 AVRSGADMSIFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420
           A+RSGADM +FYSCSFEG+QDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR
Sbjct: 361 ALRSGADMCVFYSCSFEGFQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420

Query: 421 LPRPGQSNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASNSYMNKTYLGRPWKQYSRTV 480
           LPRPGQ+NMITAQGRSDPNQNTGTSIHNCTIRAT +LAAS+SYMNKTYLGRPWKQYSRTV
Sbjct: 421 LPRPGQANMITAQGRSDPNQNTGTSIHNCTIRATPELAASSSYMNKTYLGRPWKQYSRTV 480

Query: 481 YMQTFIDGFINSAGWDPWSGEYLSTLYYGEYNNTGAGSNIKNRVTWPGYHVIYNVTNAVN 531
           YMQTFIDGF+N  GWDPW+GEYLSTLYYGEYNNTG GS+ KNRVTW GYHVI NVT+A N
Sbjct: 481 YMQTFIDGFVNPKGWDPWTGEYLSTLYYGEYNNTGGGSDTKNRVTWAGYHVINNVTDAAN 540

BLAST of Tan0005332 vs. ExPASy TrEMBL
Match: A0A5A7TAP2 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G005740 PE=3 SV=1)

HSP 1 Score: 893.6 bits (2308), Expect = 3.9e-256
Identity = 454/565 (80.35%), Postives = 488/565 (86.37%), Query Frame = 0

Query: 1   MACKFLFPPLVASFLILLTLFSSSSAANDSL--------FCNFTSNPSYCISVLQKS-TG 60
           MACKFLFPPL   FLILLTLF+SS A++DSL         C  TSNPSYCI+VL++S  G
Sbjct: 1   MACKFLFPPL---FLILLTLFTSSFASDDSLTNIFPTKAICKLTSNPSYCITVLKQSRDG 60

Query: 61  NVFDSGRFSMRRSLSKASRFLDLIEKHLHNSSTLPKSVAGALTDCQYLAQLNINFLSNSF 120
           N++DSGRFS+RRSLSKASRFLDLIEKHL NSSTLPKS+ GAL DCQYLAQLN+NFLSNSF
Sbjct: 61  NIYDSGRFSIRRSLSKASRFLDLIEKHLQNSSTLPKSIIGALKDCQYLAQLNMNFLSNSF 120

Query: 121 RAINGTDKKLTFSKADYVQSLLSAILTNIDTCLDGLKTVASGSSLEKDLLAPLIDCTKSY 180
           RA+NGTD+KLT+SKADY+QSLLSAILTNIDTCLDGL TVASGSSLEKDLLAPLIDCTKSY
Sbjct: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180

Query: 181 SLSLDLFTKGWVPR-NRNRTLEHPVKKHLQFRKGPLPLRMSRHDRAVYNSMVQETSLPSQ 240
           SLSLDLFTKGWVPR NRNRTLEHP KKHLQFRKGPLPLRMSRHDRAVYNS+     L S 
Sbjct: 181 SLSLDLFTKGWVPRWNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSS 240

Query: 241 K---LLMQLQTILS----------------------AANGYFLIYIMAGVYQEYVSIPSK 300
               +L+    ++S                      AANGYFLIYI AGVYQEYVS+PSK
Sbjct: 241 SDDGVLVNGIAVVSQDGEGDFLNITDAINAAPNNSLAANGYFLIYITAGVYQEYVSVPSK 300

Query: 301 KKYLLMIGDGINQTIVTGNRSVADGWTTFNSATFAVAAEGFMAVNITFQNRAGAIKGQAV 360
           KKYLLMIGDGINQTI+TGNRSVADGWTTFNSATFAVAAEGFMAVNITFQN AGAIKGQAV
Sbjct: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAV 360

Query: 361 AVRSGADMSIFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420
           A+RSGADM +FYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR
Sbjct: 361 ALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420

Query: 421 LPRPGQSNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASNSYMNKTYLGRPWKQYSRTV 480
           LP P Q+NMITAQGRSDPNQNTGTSIHNCTI+AT DLAAS+S+MNKTYLGRPWKQYSRTV
Sbjct: 421 LPGPRQANMITAQGRSDPNQNTGTSIHNCTIQATPDLAASSSHMNKTYLGRPWKQYSRTV 480

Query: 481 YMQTFIDGFINSAGWDPWSGEYLSTLYYGEYNNTGAGSNIKNRVTWPGYHVIYNVTNAVN 531
           YMQTFIDGF+N  GWDPWSGEYLSTLYYGEYNNTG GS+ KNRV WPGYHVI N T+A N
Sbjct: 481 YMQTFIDGFVNPKGWDPWSGEYLSTLYYGEYNNTGGGSDTKNRVIWPGYHVINNATDAAN 540

BLAST of Tan0005332 vs. ExPASy TrEMBL
Match: A0A1S4E6G9 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103503665 PE=3 SV=1)

HSP 1 Score: 893.6 bits (2308), Expect = 3.9e-256
Identity = 454/565 (80.35%), Postives = 488/565 (86.37%), Query Frame = 0

Query: 1   MACKFLFPPLVASFLILLTLFSSSSAANDSL--------FCNFTSNPSYCISVLQKS-TG 60
           MACKFLFPPL   FLILLTLF+SS A++DSL         C  TSNPSYCI+VL++S  G
Sbjct: 1   MACKFLFPPL---FLILLTLFTSSFASDDSLTNIFPTKAICKLTSNPSYCITVLKQSRDG 60

Query: 61  NVFDSGRFSMRRSLSKASRFLDLIEKHLHNSSTLPKSVAGALTDCQYLAQLNINFLSNSF 120
           N++DSGRFS+RRSLSKASRFLDLIEKHL NSSTLPKS+ GAL DCQYLAQLN+NFLSNSF
Sbjct: 61  NIYDSGRFSIRRSLSKASRFLDLIEKHLQNSSTLPKSIIGALKDCQYLAQLNMNFLSNSF 120

Query: 121 RAINGTDKKLTFSKADYVQSLLSAILTNIDTCLDGLKTVASGSSLEKDLLAPLIDCTKSY 180
           RA+NGTD+KLT+SKADY+QSLLSAILTNIDTCLDGL TVASGSSLEKDLLAPLIDCTKSY
Sbjct: 121 RAVNGTDRKLTYSKADYIQSLLSAILTNIDTCLDGLNTVASGSSLEKDLLAPLIDCTKSY 180

Query: 181 SLSLDLFTKGWVPR-NRNRTLEHPVKKHLQFRKGPLPLRMSRHDRAVYNSMVQETSLPSQ 240
           SLSLDLFTKGWVPR NRNRTLEHP KKHLQFRKGPLPLRMSRHDRAVYNS+     L S 
Sbjct: 181 SLSLDLFTKGWVPRWNRNRTLEHPGKKHLQFRKGPLPLRMSRHDRAVYNSVANRRKLSSS 240

Query: 241 K---LLMQLQTILS----------------------AANGYFLIYIMAGVYQEYVSIPSK 300
               +L+    ++S                      AANGYFLIYI AGVYQEYVS+PSK
Sbjct: 241 SDDGVLVNGIAVVSQDGEGDFLNITDAINAAPNNSLAANGYFLIYITAGVYQEYVSVPSK 300

Query: 301 KKYLLMIGDGINQTIVTGNRSVADGWTTFNSATFAVAAEGFMAVNITFQNRAGAIKGQAV 360
           KKYLLMIGDGINQTI+TGNRSVADGWTTFNSATFAVAAEGFMAVNITFQN AGAIKGQAV
Sbjct: 301 KKYLLMIGDGINQTIITGNRSVADGWTTFNSATFAVAAEGFMAVNITFQNTAGAIKGQAV 360

Query: 361 AVRSGADMSIFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420
           A+RSGADM +FYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR
Sbjct: 361 ALRSGADMCVFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPR 420

Query: 421 LPRPGQSNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASNSYMNKTYLGRPWKQYSRTV 480
           LP P Q+NMITAQGRSDPNQNTGTSIHNCTI+AT DLAAS+S+MNKTYLGRPWKQYSRTV
Sbjct: 421 LPGPRQANMITAQGRSDPNQNTGTSIHNCTIQATPDLAASSSHMNKTYLGRPWKQYSRTV 480

Query: 481 YMQTFIDGFINSAGWDPWSGEYLSTLYYGEYNNTGAGSNIKNRVTWPGYHVIYNVTNAVN 531
           YMQTFIDGF+N  GWDPWSGEYLSTLYYGEYNNTG GS+ KNRV WPGYHVI N T+A N
Sbjct: 481 YMQTFIDGFVNPKGWDPWSGEYLSTLYYGEYNNTGGGSDTKNRVIWPGYHVINNATDAAN 540

BLAST of Tan0005332 vs. ExPASy TrEMBL
Match: A0A6J1J323 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111480894 PE=3 SV=1)

HSP 1 Score: 880.6 bits (2274), Expect = 3.4e-252
Identity = 437/523 (83.56%), Postives = 462/523 (88.34%), Query Frame = 0

Query: 33  CNFTSNPSYCISVLQKSTGNVFDSGRFSMRRSLSKASRFLDLIEKHLHNSSTLPKSVAGA 92
           C FTSNPSYCISVLQKS+GNV++SGRFSMRRSL+KA+ FL+LIEKHL NSSTLPKSV G 
Sbjct: 21  CKFTSNPSYCISVLQKSSGNVYESGRFSMRRSLAKATTFLNLIEKHLQNSSTLPKSVTGG 80

Query: 93  LTDCQYLAQLNINFLSNSFRAINGTDKKLTFSKADYVQSLLSAILTNIDTCLDGLKTVAS 152
           L DCQYLAQLNINFLSNSFRA+NGTDKKLT+SKADY+QSLLSAILTNIDTCLDGL TVAS
Sbjct: 81  LKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTNIDTCLDGLNTVAS 140

Query: 153 GSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTLEHPVKKHLQFRKGPLPLRMSR 212
           GSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRT +HPV KHL+FRKGPLPLRMS+
Sbjct: 141 GSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRDHPVTKHLRFRKGPLPLRMSK 200

Query: 213 HDRAVYNSMVQETSLPSQK---LLMQLQTILS----------------------AANGYF 272
           HDRAVYNS+     L S     +L+    ++S                      A NGYF
Sbjct: 201 HDRAVYNSVANRRRLSSSSDDGILVNGIVVVSQDGTGNFLTITEAVNAAPNNSLAVNGYF 260

Query: 273 LIYIMAGVYQEYVSIPSKKKYLLMIGDGINQTIVTGNRSVADGWTTFNSATFAVAAEGFM 332
           LIYI AGVYQEYVSIPSKKKYLLMIGDGINQTI+TGN+SVADGWTTFNSATFAVAAEGFM
Sbjct: 261 LIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSVADGWTTFNSATFAVAAEGFM 320

Query: 333 AVNITFQNRAGAIKGQAVAVRSGADMSIFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVD 392
           AVNITFQN AGAIKGQAVA+RSGADMSIFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVD
Sbjct: 321 AVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVD 380

Query: 393 FIFGNAAVVFQNCNIYPRLPRPGQSNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASNS 452
           FIFGNAAVVFQNCNIYPRLPR GQ+NMITAQGRSDPNQNTGTSIHNCTIRATSDLAAS S
Sbjct: 381 FIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASKS 440

Query: 453 YMNKTYLGRPWKQYSRTVYMQTFIDGFINSAGWDPWSGEYLSTLYYGEYNNTGAGSNIKN 512
           YMNKTYLGRPWKQYSRTVYMQTFIDGF+N AGWDPWSGEYLSTLYY EYNNTGAGSN KN
Sbjct: 441 YMNKTYLGRPWKQYSRTVYMQTFIDGFVNPAGWDPWSGEYLSTLYYAEYNNTGAGSNTKN 500

Query: 513 RVTWPGYHVIYNVTNAVNFTVSNLLFGDVWLPPSDVPYTGGFM 531
           RVTWPGYHVI N T+A NFTVSN L GD WLPP+ VPYTGGF+
Sbjct: 501 RVTWPGYHVISNATDASNFTVSNFLVGDAWLPPTGVPYTGGFI 543

BLAST of Tan0005332 vs. ExPASy TrEMBL
Match: A0A6J1FJR9 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111446371 PE=3 SV=1)

HSP 1 Score: 880.6 bits (2274), Expect = 3.4e-252
Identity = 439/523 (83.94%), Postives = 461/523 (88.15%), Query Frame = 0

Query: 33  CNFTSNPSYCISVLQKSTGNVFDSGRFSMRRSLSKASRFLDLIEKHLHNSSTLPKSVAGA 92
           C FTSNPSYCISVLQKS+GNV++SGRFSMRRSLSKAS FL+LIEKHL NSSTLPKSV G 
Sbjct: 21  CKFTSNPSYCISVLQKSSGNVYESGRFSMRRSLSKASAFLNLIEKHLQNSSTLPKSVTGG 80

Query: 93  LTDCQYLAQLNINFLSNSFRAINGTDKKLTFSKADYVQSLLSAILTNIDTCLDGLKTVAS 152
           L DCQYLAQLNINFLSNSFRA+NGTDKKLT+SKADY+QSLLSAILTNIDTCLDGL TVAS
Sbjct: 81  LKDCQYLAQLNINFLSNSFRAVNGTDKKLTYSKADYIQSLLSAILTNIDTCLDGLNTVAS 140

Query: 153 GSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTLEHPVKKHLQFRKGPLPLRMSR 212
           GSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRT +HPV KHL+FRKGPLPLRMS+
Sbjct: 141 GSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVPRNRNRTRDHPVTKHLRFRKGPLPLRMSK 200

Query: 213 HDRAVYNSMVQETSLPSQK---LLMQLQTILS----------------------AANGYF 272
           HDRAVYNS+     L S     +L+    ++S                      A NGYF
Sbjct: 201 HDRAVYNSVANRRRLLSSSDDGILVNGIVVVSQDGTGNFLTITEAVNAAPNNSLAVNGYF 260

Query: 273 LIYIMAGVYQEYVSIPSKKKYLLMIGDGINQTIVTGNRSVADGWTTFNSATFAVAAEGFM 332
           LIYI AGVYQEYVSIPSKKKYLLMIGDGINQTI+TGN+SVADGWTTFNSATFAVAAEGFM
Sbjct: 261 LIYIAAGVYQEYVSIPSKKKYLLMIGDGINQTIITGNQSVADGWTTFNSATFAVAAEGFM 320

Query: 333 AVNITFQNRAGAIKGQAVAVRSGADMSIFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVD 392
           AVNITFQN AGAIKGQAVA+RSGADMSIFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVD
Sbjct: 321 AVNITFQNTAGAIKGQAVAMRSGADMSIFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVD 380

Query: 393 FIFGNAAVVFQNCNIYPRLPRPGQSNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASNS 452
           FIFGNAAVVFQNCNIYPRLPR GQ+NMITAQGRSDPNQNTGTSIHNCTIRATSDLAAS S
Sbjct: 381 FIFGNAAVVFQNCNIYPRLPRSGQTNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASKS 440

Query: 453 YMNKTYLGRPWKQYSRTVYMQTFIDGFINSAGWDPWSGEYLSTLYYGEYNNTGAGSNIKN 512
           YMNKTYLGRPWKQYSRTVYMQTFIDGFIN AGWDPWSGEYLSTLYY EYNN+GAGSN KN
Sbjct: 441 YMNKTYLGRPWKQYSRTVYMQTFIDGFINPAGWDPWSGEYLSTLYYAEYNNSGAGSNTKN 500

Query: 513 RVTWPGYHVIYNVTNAVNFTVSNLLFGDVWLPPSDVPYTGGFM 531
           RVTWPGYHVI N T A NFTVSN L GD WLPP+ VPYTGGF+
Sbjct: 501 RVTWPGYHVISNATEASNFTVSNFLVGDAWLPPTGVPYTGGFI 543

BLAST of Tan0005332 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 577.8 bits (1488), Expect = 9.0e-165
Identity = 296/536 (55.22%), Postives = 375/536 (69.96%), Query Frame = 0

Query: 25  SAANDSLFCNFTSNPSYCISVLQKSTGNVFDSGRFSMRRSLSKASRFLDLIEKHL-HNSS 84
           +A + S  C F  +PSYC SVL    G+++  GR S+RRSLS+A RF+ +I+  L     
Sbjct: 31  NATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGRLSLRRSLSRARRFISMIDAELDRKGK 90

Query: 85  TLPKSVAGALTDCQYLAQLNINFLSNSFRAINGTDKKLTFSKADYVQSLLSAILTNIDTC 144
              KS  GAL DC++LA L +++L +S +  + T K L+ S+A+ V + LSA +TN  TC
Sbjct: 91  VAAKSTVGALEDCKFLASLTMDYLLSSSQTADST-KTLSLSRAEDVHTFLSAAITNEQTC 150

Query: 145 LDGLKTVASGSSLEKDLLAPLIDCTKSYSLSLDLFTKGWVP-RNRNRTLEHP---VKKHL 204
           L+GLK+ AS + L  D    L + TK Y +SL LF+KGWVP R R+R +  P    KK  
Sbjct: 151 LEGLKSTASENGLSGD----LFNDTKLYGVSLALFSKGWVPRRQRSRPIWQPQARFKKFF 210

Query: 205 QFRKGPLPLRMSRHDRAVYNSMVQETSLPSQKLLMQLQTILS------------------ 264
            FR G LPL+M+   RAVYN++ +   L S    +Q+  I++                  
Sbjct: 211 GFRNGKLPLKMTERARAVYNTVTRRKLLQSDADAVQVSDIVTVIQNGTGNFTTINAAIAA 270

Query: 265 ------AANGYFLIYIMAGVYQEYVSIPSKKKYLLMIGDGINQTIVTGNRSVADGWTTFN 324
                  +NGYFLIY+ AG+Y+EYV +P  K+Y++MIGDGINQT++TGNRSV DGWTTFN
Sbjct: 271 APNKTDGSNGYFLIYVTAGLYEEYVEVPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFN 330

Query: 325 SATFAVAAEGFMAVNITFQNRAGAIKGQAVAVRSGADMSIFYSCSFEGYQDTLYTHSLRQ 384
           SATF ++   F+ VNIT +N AG  KGQAVA+RSG D+S+FYSCSFE YQDTLYTHSLRQ
Sbjct: 331 SATFILSGPNFIGVNITIRNTAGPTKGQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQ 390

Query: 385 FFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPGQSNMITAQGRSDPNQNTGTSIHNCT 444
           F+RECD+YGTVDFIFGNAAVV QNCN+YPR PR GQSN +TAQGR+DPNQNTGT+IH CT
Sbjct: 391 FYRECDVYGTVDFIFGNAAVVLQNCNLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCT 450

Query: 445 IRATSDLAASNSYMNKTYLGRPWKQYSRTVYMQTFIDGFINSAGWDPWSGEY-LSTLYYG 504
           IR   DLA SN Y  KTYLGRPWK+YSRTV MQT+IDGF+  +GW+ WSG++ LSTLYY 
Sbjct: 451 IRPADDLATSN-YTVKTYLGRPWKEYSRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYA 510

Query: 505 EYNNTGAGSNIKNRVTWPGYHVIYNVTNAVNFTVSNLLFGDVWLPPSDVPYTGGFM 531
           EYNNTG GS+  NRVTWPGYHVI N T+A NFTV+N L G+ W+  + VP+ GG +
Sbjct: 511 EYNNTGPGSDTTNRVTWPGYHVI-NATDASNFTVTNFLVGEGWIGQTGVPFVGGLI 559

BLAST of Tan0005332 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 560.5 bits (1443), Expect = 1.5e-159
Identity = 306/567 (53.97%), Postives = 382/567 (67.37%), Query Frame = 0

Query: 10  LVASFLILLTLFSSSS----AANDSLFCNFTSNPSYCISVL-QKSTGNVFDSGRFSMRRS 69
           LV  FL L TLF +S     + + S  C  T +P +C SV  Q S G+V + GRFS+R+S
Sbjct: 8   LVTLFLSLQTLFIASQTLLPSNSSSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLRKS 67

Query: 70  LSKASRFLDLIEKHL-HNSSTLPKSVAGALTDCQYLAQLNINFLSNSFRAINGT--DKKL 129
           L+++ +F   I+++L  N++ L +S  GAL DC+YLA L  ++L  SF  +N T   K L
Sbjct: 68  LTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSKTL 127

Query: 130 TFSKADYVQSLLSAILTNIDTCLDGLKTVASGS-SLEKDLLAPLIDCTKSYSLSLDLFTK 189
           +FSKAD +Q+LLSA LTN  TCLDG+ T AS S ++   +  PLI+ TK +S+SL LFTK
Sbjct: 128 SFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLINDTKLFSVSLALFTK 187

Query: 190 GWVPRNRNRTLE----HPVKKH------LQFRKGPLPLRMSRHDRAVYNS---------- 249
           GWVP+ + +       HP   H        FR G LPL+M+ H RAVY S          
Sbjct: 188 GWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYESLSRRKLADDD 247

Query: 250 ------MVQETSLPSQKLLMQLQTILSAAN----------GYFLIYIMAGVYQEYVSIPS 309
                 +V +    +Q       TI  A N          GYF+IY+ +GVY+E V I  
Sbjct: 248 NDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYFVIYVTSGVYEENVVIAK 307

Query: 310 KKKYLLMIGDGINQTIVTGNRSVADGWTTFNSATFAVAAEGFMAVNITFQNRAGAIKGQA 369
            K+YL+MIGDGIN+T+VTGNR+V DGWTTFNSATFAV +  F+AVN+TF+N AG  K QA
Sbjct: 308 NKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFVAVNMTFRNTAGPEKHQA 367

Query: 370 VAVRSGADMSIFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYP 429
           VA+RS AD+SIFYSCSFE YQDTLYTHSLRQF+RECDIYGTVDFIFGNAAVVFQ+CN+YP
Sbjct: 368 VAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQDCNLYP 427

Query: 430 RLPRPGQSNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASNSYMNKTYLGRPWKQYSRT 489
           R P   Q N ITAQGR+DPNQNTG SIHNCTI+   DL +SN Y  KTYLGRPWK+YSRT
Sbjct: 428 RQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSN-YTVKTYLGRPWKEYSRT 487

Query: 490 VYMQTFIDGFINSAGWDPWSGEY-LSTLYYGEYNNTGAGSNIKNRVTWPGYHVIYNVTNA 531
           V+MQ++ID  +   GW  W+G++ LSTLYY EYNNTG+GS+  +RV WPGYHVI N T+A
Sbjct: 488 VFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTDRVVWPGYHVI-NSTDA 547

BLAST of Tan0005332 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 557.4 bits (1435), Expect = 1.3e-158
Identity = 301/576 (52.26%), Postives = 378/576 (65.62%), Query Frame = 0

Query: 6   LFPPLVASFLILLTLFSSSSAANDSLFCNFTSNPSYCISVLQKSTGNVFDSGRFSMRRSL 65
           +F  +  SF +   L SS + +N S  C  T +P YC SV   S GNV   G FS+R+SL
Sbjct: 5   IFILITLSFFLQSVLASSQTLSNSSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSIRKSL 64

Query: 66  SKASRFLDLIEKHLHNSSTLPK-SVAGALTDCQYLAQLNINFLSNSFRAINGTD-----K 125
           S++ +F+  +++++  ++ L + +V  AL DC++LA L +++L  SF  +N T      K
Sbjct: 65  SQSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAKTSFK 124

Query: 126 KLTFSKADYVQSLLSAILTNIDTCLDGLKTVASGS---SLEKDLLAPLIDCTKSYSLSLD 185
            L+F KAD +Q+LLSA LTN  TCL+GL T AS S   ++   +  PL++ TK   +SL 
Sbjct: 125 PLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVNDTKLLGVSLA 184

Query: 186 LFTKGWVPRNRNRT----------LEHPVKKHLQFRKGPLPLRMSRHDRAVYNSMVQETS 245
           LFTKGWVP+ + R                K    FR G LPL+M+   +AVY S+ +   
Sbjct: 185 LFTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKTKAVYESLSRRKL 244

Query: 246 LP----------SQKLLMQLQTI---------------------LSAANGYFLIYIMAGV 305
                       S  L+  + T+                        + G+FLIY+ AG+
Sbjct: 245 ADGDSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNNTDGSAGFFLIYVTAGI 304

Query: 306 YQEYVSIPSKKKYLLMIGDGINQTIVTGNRSVADGWTTFNSATFAVAAEGFMAVNITFQN 365
           Y+EY+SI   K+Y++MIGDGINQT+VTGNRSV DGWTTFNSATFAV A  F+AVNITF+N
Sbjct: 305 YEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATFAVTAPNFVAVNITFRN 364

Query: 366 RAGAIKGQAVAVRSGADMSIFYSCSFEGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAV 425
            AG  K QAVA+RSGAD SIFYSCSFE YQDTLYTHSLRQF+RECD+YGTVDFIFGNAAV
Sbjct: 365 TAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAV 424

Query: 426 VFQNCNIYPRLPRPGQSNMITAQGRSDPNQNTGTSIHNCTIRATSDLAASNSYMNKTYLG 485
           VFQNCN+YPR P P Q N ITAQGRSDPNQNTGTSI NCTI+   DL +SN Y  KTYLG
Sbjct: 425 VFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPADDLVSSN-YTVKTYLG 484

Query: 486 RPWKQYSRTVYMQTFIDGFINSAGWDPWSGEY-LSTLYYGEYNNTGAGSNIKNRVTWPGY 531
           RPWK+YSRTVYMQ++IDGF+   GW  W+G++ LSTLYY EYNNTG GSN  NRVTWPGY
Sbjct: 485 RPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNTGPGSNTTNRVTWPGY 544

BLAST of Tan0005332 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 414.8 bits (1065), Expect = 1.0e-115
Identity = 236/548 (43.07%), Postives = 327/548 (59.67%), Query Frame = 0

Query: 24  SSAANDSLFCNFTSNPSYCISVL---QKSTGNVFDSGRFSMRRSLSKASRFLDLIEKHLH 83
           S + + SL C  T  P  C ++L   + S  + +  G+F++++ L +ASR   +I  +  
Sbjct: 74  SQSQSPSLACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQASRLSKVITSYAR 133

Query: 84  NSSTLPKSVA----GALTDCQYLAQLNINFLSNSFRAINGTDKKLTFSKADYVQSLLSAI 143
              + P S      GA+ DC  L++L++N+L      +  T + +T +  ++V SLLS +
Sbjct: 134 RVESKPGSATAEEIGAVADCGELSELSVNYLETVTTELK-TAQVMTAALVEHVNSLLSGV 193

Query: 144 LTNIDTCLDGLKTVASGSSLEKDLLAPLIDCTKSYSLSLDLF-------------TKGWV 203
           +TN  TCLDGL    SG +    + +P+ + T+ YS+SL L              +KG +
Sbjct: 194 VTNQQTCLDGLVEAKSGFAAA--IGSPMGNLTRLYSISLGLVSHALNRNLKRFKASKGKI 253

Query: 204 PRNRNRTLEHPVKKHLQ-FRK---GPLPLRMSRHDRAVYNSMVQETSLPSQKLLM----- 263
               N T   P++  ++  RK        R +  +          + L S+ +++     
Sbjct: 254 LGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGSILVSKAVIVGPFKS 313

Query: 264 -QLQTILSA----------ANGYFLIYIMAGVYQEYVSIPSKKKYLLMIGDGINQTIVTG 323
               TI  A           +GYF+IY   GVY+EY+ +P  KK L+++GDGIN+TI+TG
Sbjct: 314 DNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNLMLMGDGINKTIITG 373

Query: 324 NRSVADGWTTFNSATFAVAAEGFMAVNITFQNRAGAIKGQAVAVRSGADMSIFYSCSFEG 383
           N +V DGWTT+N ++FAV  E FMAV++TF+N AG  K QAVA+R+ A+ S FY CSFEG
Sbjct: 374 NHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRNNAEGSSFYRCSFEG 433

Query: 384 YQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPGQSNMITAQGRSDP 443
           YQDTLY HSLRQF+RECDIYGTVDFIFGNAA +FQNCNIY R P   Q N ITA GR DP
Sbjct: 434 YQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMAKQKNAITAHGRLDP 493

Query: 444 NQNTGTSIHNCTIRATSDLAASNSYMNKTYLGRPWKQYSRTVYMQTFIDGFINSAGWDPW 503
           NQNTG SI NCTI+A  DLAA       T+LGRPWK YSRTV+MQ++I   +   GW  W
Sbjct: 494 NQNTGISIINCTIKAAPDLAAEPK-SAMTFLGRPWKPYSRTVFMQSYISDIVQPVGWLEW 553

Query: 504 SGEY-LSTLYYGEYNNTGAGSNIKNRVTWPGYHVIYNVTNAVNFTVSNLLFGDVWLPPSD 531
           +G   L T+YYGEY+N G G+N   RV W GY+++ N+  A+NFTV N   GD WLP +D
Sbjct: 554 NGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLL-NLAEAMNFTVYNFTMGDTWLPQTD 613

BLAST of Tan0005332 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 397.9 bits (1021), Expect = 1.3e-110
Identity = 229/550 (41.64%), Postives = 319/550 (58.00%), Query Frame = 0

Query: 22  SSSSAANDSLFCNFTSNPSYC---ISVLQKSTGNVFDSGRFSMRRSLSKASRFLDLIEKH 81
           S  S    ++ C  T  P  C   +S ++ S  + +  G+F+M++ L +A R   +I + 
Sbjct: 77  SQPSPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRF 136

Query: 82  LH----NSSTLPKSVAGALTDCQYLAQLNINFLSNSFRAINGTDKKLTFSKADYVQSLLS 141
                 +  T       A+ DC  LA+L++ +L      +   +  +T +  D V SLL 
Sbjct: 137 AQRVEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELKAAE-LMTAALVDRVTSLLG 196

Query: 142 AILTNIDTCLDGLKTVASGSSLEKDLLAPLIDCTKSYSLSLDLF-------------TKG 201
            ++TN  TCLDGL    SG +    +  PL + T+ YS+SL L              +KG
Sbjct: 197 GVVTNQQTCLDGLVDAKSGFATA--IGTPLGNLTRLYSVSLGLVSHALNRNLKRYKGSKG 256

Query: 202 WVPRNRNRTLEHPVKKHLQF-----RKGPLPLRMSRH-----DRAVYNSMVQETSLPSQK 261
            +    N+ +  P++  ++       KG    + +R+     + +  + +V+E       
Sbjct: 257 KIFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPY 316

Query: 262 LLMQLQTILSAA----------NGYFLIYIMAGVYQEYVSIPSKKKYLLMIGDGINQTIV 321
                 TI  A            GYF+IY  AG+Y+EYV I +KK+ +++IGDGIN+TI+
Sbjct: 317 ETDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTII 376

Query: 322 TGNRSVADGWTTFNSATFAVAAEGFMAVNITFQNRAGAIKGQAVAVRSGADMSIFYSCSF 381
           +GN S  DGWTT+NS+TFAV  + F+AV++TF+N AG  K QAVAVR+ AD S FY CSF
Sbjct: 377 SGNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSF 436

Query: 382 EGYQDTLYTHSLRQFFRECDIYGTVDFIFGNAAVVFQNCNIYPRLPRPGQSNMITAQGRS 441
           EGYQDTLY HSLRQF+RECDIYGT+DFIFGNAA +FQNCNIY R P   Q N +TA GR+
Sbjct: 437 EGYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRT 496

Query: 442 DPNQNTGTSIHNCTIRATSDLAASNSYMNKTYLGRPWKQYSRTVYMQTFIDGFINSAGWD 501
           DPNQ TG SI NCTI A  DLAA +     T+LGRPWK YSRTVY+Q++I   +   GW 
Sbjct: 497 DPNQKTGISIINCTIGAAPDLAA-DPKSTMTFLGRPWKPYSRTVYIQSYISDVVQPVGWL 556

Query: 502 PWSGEY-LSTLYYGEYNNTGAGSNIKNRVTWPGYHVIYNVTNAVNFTVSNLLFGDVWLPP 531
            W+G   L T+ YGEY+N G G++   RV W GY ++ N+  A+NFTV N   GD WLP 
Sbjct: 557 EWNGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLL-NLVQAMNFTVYNFTLGDTWLPQ 616