Tan0005218 (gene) Snake gourd v1

Overview


Name	Tan0005218
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	DPH4 homolog
Location	LG02: 86765161 .. 86765703 (+)
RNA-Seq Expression	Tan0005218
Synteny	Tan0005218

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAGTTCAAGCAGTGGCATCATTGGTGAAACTTACTATGATGTACTATCTTTGAGAGAAGATGCTAGCCACGACGAAATTCGAGCTAACTACCGGTCTGCCCTCCTTAATTTCCACCCTGATAAGTTACAAGCTATGTGCCAGAGATCTCATCCTGACGATATCATGGGAGAACGTTACTTCAAGGTGCAGAAGGCTTGGGAAGTACTCGGAAGCTCTACATCTCGTGCAGCTTATGACCGAGAGCTCCAAGCTGCCAAAGGGGATGCAATGGATGCAGAGAGCATAAGCTTAGAGGATATGGTTGTGGAAGGTAAAGGTGAGGTTGCAGAACTCTTTTATCAGTGCCGTTGTGGTGATTTCTTCTTTATTGATTCTGGGGAGTTGGAGGAAATGGGATATCCATTGTTGAGGAATGGGAGTAAAGTTTCTTTAAGGACATTGGATGCTTTGCCTGCATCTGTTGTTTTGCCTTGTGGTTCTTGTTCTTTGAAAGTTCGTTTGCTGATTGATTCGAATTCAAGCGTGTCGATTGATTGTTGA

mRNA sequence

ATGAGTTCAAGCAGTGGCATCATTGGTGAAACTTACTATGATGTACTATCTTTGAGAGAAGATGCTAGCCACGACGAAATTCGAGCTAACTACCGGTCTGCCCTCCTTAATTTCCACCCTGATAAGTTACAAGCTATGTGCCAGAGATCTCATCCTGACGATATCATGGGAGAACGTTACTTCAAGGTGCAGAAGGCTTGGGAAGTACTCGGAAGCTCTACATCTCGTGCAGCTTATGACCGAGAGCTCCAAGCTGCCAAAGGGGATGCAATGGATGCAGAGAGCATAAGCTTAGAGGATATGGTTGTGGAAGGTAAAGGTGAGGTTGCAGAACTCTTTTATCAGTGCCGTTGTGGTGATTTCTTCTTTATTGATTCTGGGGAGTTGGAGGAAATGGGATATCCATTGTTGAGGAATGGGAGTAAAGTTTCTTTAAGGACATTGGATGCTTTGCCTGCATCTGTTGTTTTGCCTTGTGGTTCTTGTTCTTTGAAAGTTCGTTTGCTGATTGATTCGAATTCAAGCGTGTCGATTGATTGTTGA

Coding sequence (CDS)

ATGAGTTCAAGCAGTGGCATCATTGGTGAAACTTACTATGATGTACTATCTTTGAGAGAAGATGCTAGCCACGACGAAATTCGAGCTAACTACCGGTCTGCCCTCCTTAATTTCCACCCTGATAAGTTACAAGCTATGTGCCAGAGATCTCATCCTGACGATATCATGGGAGAACGTTACTTCAAGGTGCAGAAGGCTTGGGAAGTACTCGGAAGCTCTACATCTCGTGCAGCTTATGACCGAGAGCTCCAAGCTGCCAAAGGGGATGCAATGGATGCAGAGAGCATAAGCTTAGAGGATATGGTTGTGGAAGGTAAAGGTGAGGTTGCAGAACTCTTTTATCAGTGCCGTTGTGGTGATTTCTTCTTTATTGATTCTGGGGAGTTGGAGGAAATGGGATATCCATTGTTGAGGAATGGGAGTAAAGTTTCTTTAAGGACATTGGATGCTTTGCCTGCATCTGTTGTTTTGCCTTGTGGTTCTTGTTCTTTGAAAGTTCGTTTGCTGATTGATTCGAATTCAAGCGTGTCGATTGATTGTTGA

Protein sequence

MSSSSGIIGETYYDVLSLREDASHDEIRANYRSALLNFHPDKLQAMCQRSHPDDIMGERYFKVQKAWEVLGSSTSRAAYDRELQAAKGDAMDAESISLEDMVVEGKGEVAELFYQCRCGDFFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSIDC

Homology

[Close]

BLAST of Tan0005218 vs. ExPASy Swiss-Prot
Match: Q54CI5 (DPH4 homolog OS=Dictyostelium discoideum OX=44689 GN=dph4 PE=3 SV=1)

HSP 1 Score: 63.2 bits (152), Expect = 3.6e-09
Identity = 45/163 (27.61%), Postives = 75/163 (46.01%), Query Frame = 0


Query: 2   SSSSGIIGETYYDVLSLREDASHDEIRANYRSALLNFHPDKL--------------QAMC 61
           ++S+    + YY++L +  DA  +EI+ +YR   L +HPDKL                + 
Sbjct: 6   NNSNKCENKNYYEILKVSIDADIEEIKKSYRKLALLYHPDKLNKEENIEENINNFSNCLV 65

Query: 62  QRSHPDDIMGERYFK-VQKAWEVLGSSTSRAAYDRELQAAKGDAMD-AESISLEDMVVEG 121
             ++ ++    + F  +Q AWE L     R  YD  L   K      ++ I L+DM  E 
Sbjct: 66  NNNNNNNNSNTKDFNDIQIAWETLKDDLLRKQYDSLLLEQKRQKYSVSDEIDLDDM--EF 125

Query: 122 KGEVAELFYQCRCGDFFFIDSGELEEMGYPLLRNGSKVSLRTL 149
             E +E  Y CRCGD + I   +L E    +  +G  +S++ +
Sbjct: 126 IEENSEYVYPCRCGDHYIITEDQLSEGSDVVCCSGCSLSIKVI 166

[Close]

BLAST of Tan0005218 vs. ExPASy Swiss-Prot
Match: P47138 (Diphthamide biosynthesis protein 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=JJJ3 PE=1 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 3.9e-08
Identity = 52/158 (32.91%), Postives = 74/158 (46.84%), Query Frame = 0


Query: 11  TYYDVLSLREDASHDEIRANYRSALLNFHPDKLQAMCQRSHPDDIMGERYFKVQKAWEVL 70
           T+Y++L +  DA+ DEI+  YR+ LLN HPDKL     +S  D +      K+Q A+++L
Sbjct: 8   THYEILRIPSDATQDEIKKAYRNRLLNTHPDKL----SKSIHDTVSNVTINKIQDAYKIL 67

Query: 71  GSSTSRAAYDR-ELQAAK-------GDAMDAESISLEDMVVEGKGEVAELFYQC-RC--- 130
            +  +R  YDR  L+  K       GD +D    SL+D   +   +  E    C RC   
Sbjct: 68  SNIKTRREYDRLILENYKRQGFHNCGDGLD--EFSLDDFSFD--EDKLEFMMNCPRCQFV 127

Query: 131 GDFFF-----------IDSGELEEMGYPLLRNGSKVSL 146
           G F F           +D+ E    GY LL   S  SL
Sbjct: 128 GGFHFSESLLDECIDNVDAMERSHSGYQLLTQCSACSL 157

[Close]

BLAST of Tan0005218 vs. ExPASy Swiss-Prot
Match: Q6FWM1 (Diphthamide biosynthesis protein 4 OS=Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65) OX=284593 GN=DPH4 PE=3 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.6e-07
Identity = 49/166 (29.52%), Postives = 77/166 (46.39%), Query Frame = 0


Query: 11  TYYDVLSLREDASHDEIRANYRSALLNFHPDKLQAMCQRSHPDDIMGERYFKVQKAWEVL 70
           ++Y+VL +  DAS DEI+  YR  LL  HPDK + +   S      G    ++Q+A+ VL
Sbjct: 10  SHYEVLGVPVDASTDEIKLTYRKMLLTLHPDKSKTVPIDSTIKIRSGVSINQIQEAYRVL 69

Query: 71  GSSTSRAAYDRELQAAK--------GDAMDAESISLEDMVVEGKGEVAELFYQCRCGDFF 130
            +   RAAY+R L  +         GD +D  ++  E    E K E      +C+ G  F
Sbjct: 70  SNDQLRAAYNRALNDSNKLAGFNNFGDGLDEFNLD-EFEFNEEKLEYVMTCPRCQSGGGF 129

Query: 131 FIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRL 169
            +    LEE     + +G    L   +     V+  C +CSL +++
Sbjct: 130 VLSEDMLEE----CIEDG----LTESEEQGYQVLTQCTACSLWLKV 166

[Close]

BLAST of Tan0005218 vs. ExPASy Swiss-Prot
Match: B9KH92 (Chaperone protein DnaJ OS=Anaplasma marginale (strain Florida) OX=320483 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 4.4e-07
Identity = 29/74 (39.19%), Postives = 43/74 (58.11%), Query Frame = 0


Query: 9  GETYYDVLSLREDASHDEIRANYRSALLNFHPDKLQAMCQRSHPDDIMGERYF-KVQKAW 68
          G  YY++L +  +AS +EI+ +YR  +  +HPDK        +P D   E  F K+ +A+
Sbjct: 3  GNDYYEILEVSRNASAEEIKKSYRKMVFKYHPDK--------NPGDKKAEEKFKKISEAY 62

Query: 69 EVLGSSTSRAAYDR 82
          EVL +   RAAYDR
Sbjct: 63 EVLSNPEKRAAYDR 68

[Close]

BLAST of Tan0005218 vs. ExPASy Swiss-Prot
Match: Q5P9E0 (Chaperone protein DnaJ OS=Anaplasma marginale (strain St. Maries) OX=234826 GN=dnaJ PE=3 SV=1)

HSP 1 Score: 56.2 bits (134), Expect = 4.4e-07
Identity = 29/74 (39.19%), Postives = 43/74 (58.11%), Query Frame = 0


Query: 9  GETYYDVLSLREDASHDEIRANYRSALLNFHPDKLQAMCQRSHPDDIMGERYF-KVQKAW 68
          G  YY++L +  +AS +EI+ +YR  +  +HPDK        +P D   E  F K+ +A+
Sbjct: 3  GNDYYEILEVSRNASAEEIKKSYRKMVFKYHPDK--------NPGDKKAEEKFKKISEAY 62

Query: 69 EVLGSSTSRAAYDR 82
          EVL +   RAAYDR
Sbjct: 63 EVLSNPEKRAAYDR 68

[Close]

BLAST of Tan0005218 vs. NCBI nr
Match: XP_023523039.1 (DPH4 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 326.6 bits (836), Expect = 1.3e-85
Identity = 160/180 (88.89%), Postives = 169/180 (93.89%), Query Frame = 0


Query: 1   MSSSSGIIGETYYDVLSLREDASHDEIRANYRSALLNFHPDKLQAMCQRSHPDDIMGERY 60
           MSSSSG IGETYYDVLSLREDASHDEIRA+YRSALLNFHPDKLQAMCQ+SHPDD++GERY
Sbjct: 1   MSSSSGSIGETYYDVLSLREDASHDEIRASYRSALLNFHPDKLQAMCQKSHPDDVVGERY 60

Query: 61  FKVQKAWEVLGSSTSRAAYDRELQAAKGDAMDAESISLEDMVVEGKGEVAELFYQCRCGD 120
           FKVQKAWEVLGSS SRAAYDRELQAAKGDAM AESI LEDM VE KGE+ ELFYQCRCGD
Sbjct: 61  FKVQKAWEVLGSSMSRAAYDRELQAAKGDAMGAESIGLEDMAVEDKGEIVELFYQCRCGD 120

Query: 121 FFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSIDC 180
           +FFIDSGELEEMGYP+LR GSKVSLRT DALPAS+VLPCGSCSLKVRLLIDS+S  SIDC
Sbjct: 121 YFFIDSGELEEMGYPMLRIGSKVSLRTFDALPASIVLPCGSCSLKVRLLIDSDSCASIDC 180

[Close]

BLAST of Tan0005218 vs. NCBI nr
Match: XP_023537718.1 (DPH4 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 325.5 bits (833), Expect = 2.9e-85
Identity = 159/180 (88.33%), Postives = 169/180 (93.89%), Query Frame = 0


Query: 1   MSSSSGIIGETYYDVLSLREDASHDEIRANYRSALLNFHPDKLQAMCQRSHPDDIMGERY 60
           MSSS+G IGETYYDVLSLREDASHDEIRA+YRSALLNFHPDKLQAMCQ+SHPDD++GERY
Sbjct: 39  MSSSNGSIGETYYDVLSLREDASHDEIRASYRSALLNFHPDKLQAMCQKSHPDDVVGERY 98

Query: 61  FKVQKAWEVLGSSTSRAAYDRELQAAKGDAMDAESISLEDMVVEGKGEVAELFYQCRCGD 120
           FKVQKAWEVLGSS SRAAYDRELQAAKGDAM AESI LEDM VE KGE+ ELFYQCRCGD
Sbjct: 99  FKVQKAWEVLGSSMSRAAYDRELQAAKGDAMGAESIGLEDMAVEDKGEIVELFYQCRCGD 158

Query: 121 FFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSIDC 180
           +FFIDSGELEEMGYP+LR GSKVSLRT DALPAS+VLPCGSCSLKVRLLIDS+S  SIDC
Sbjct: 159 YFFIDSGELEEMGYPMLRIGSKVSLRTFDALPASIVLPCGSCSLKVRLLIDSDSCASIDC 218

[Close]

BLAST of Tan0005218 vs. NCBI nr
Match: XP_038884890.1 (DPH4 homolog isoform X2 [Benincasa hispida])

HSP 1 Score: 325.1 bits (832), Expect = 3.8e-85
Identity = 163/179 (91.06%), Postives = 171/179 (95.53%), Query Frame = 0


Query: 1   MSSSSGIIGETYYDVLSLREDASHDEIRANYRSALLNFHPDKLQAMCQRSHPDDIMGERY 60
           MSSSSG IGETYYDVLSL+EDAS+DEIRA+YRSALLNFHPDKLQAMC +SHPDDI  ERY
Sbjct: 1   MSSSSGSIGETYYDVLSLKEDASYDEIRASYRSALLNFHPDKLQAMCHKSHPDDIALERY 60

Query: 61  FKVQKAWEVLGSSTSRAAYDRELQAAKGDAMDAESISLEDMVVEGKGEVAELFYQCRCGD 120
           FKVQKAWEVLGSS SRA+YDRELQAAKGDA+ AESISLEDMVVE KGEV ELFYQCRCGD
Sbjct: 61  FKVQKAWEVLGSSMSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGD 120

Query: 121 FFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSID 180
           +FFIDSGEL+EMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSID
Sbjct: 121 YFFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSID 179

[Close]

BLAST of Tan0005218 vs. NCBI nr
Match: XP_023001946.1 (DPH4 homolog isoform X2 [Cucurbita maxima])

HSP 1 Score: 324.3 bits (830), Expect = 6.5e-85
Identity = 160/180 (88.89%), Postives = 170/180 (94.44%), Query Frame = 0


Query: 1   MSSSSGIIGETYYDVLSLREDASHDEIRANYRSALLNFHPDKLQAMCQRSHPDDIMGERY 60
           MSSSSG IGETYYDVLSLREDASHDEIRA+YRSALLNFHPDKLQAMCQ+SHP+D++GERY
Sbjct: 1   MSSSSGSIGETYYDVLSLREDASHDEIRASYRSALLNFHPDKLQAMCQKSHPNDVVGERY 60

Query: 61  FKVQKAWEVLGSSTSRAAYDRELQAAKGDAMDAESISLEDMVVEGKGEVAELFYQCRCGD 120
           FKVQKAWEVLGSS SRAAYDRELQAAKG+AM AESI LEDM VE KGEV EL YQCRCGD
Sbjct: 61  FKVQKAWEVLGSSMSRAAYDRELQAAKGEAMGAESIGLEDMAVEDKGEVVELLYQCRCGD 120

Query: 121 FFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSIDC 180
           +FFIDSGELEEMGYP+LR GSKVSLRTLDALPAS+VLPCGSCSLKVRLLIDS+S VSIDC
Sbjct: 121 YFFIDSGELEEMGYPMLRIGSKVSLRTLDALPASIVLPCGSCSLKVRLLIDSDSCVSIDC 180

[Close]

BLAST of Tan0005218 vs. NCBI nr
Match: XP_023001945.1 (DPH4 homolog isoform X1 [Cucurbita maxima])

HSP 1 Score: 324.3 bits (830), Expect = 6.5e-85
Identity = 160/180 (88.89%), Postives = 170/180 (94.44%), Query Frame = 0


Query: 1   MSSSSGIIGETYYDVLSLREDASHDEIRANYRSALLNFHPDKLQAMCQRSHPDDIMGERY 60
           MSSSSG IGETYYDVLSLREDASHDEIRA+YRSALLNFHPDKLQAMCQ+SHP+D++GERY
Sbjct: 40  MSSSSGSIGETYYDVLSLREDASHDEIRASYRSALLNFHPDKLQAMCQKSHPNDVVGERY 99

Query: 61  FKVQKAWEVLGSSTSRAAYDRELQAAKGDAMDAESISLEDMVVEGKGEVAELFYQCRCGD 120
           FKVQKAWEVLGSS SRAAYDRELQAAKG+AM AESI LEDM VE KGEV EL YQCRCGD
Sbjct: 100 FKVQKAWEVLGSSMSRAAYDRELQAAKGEAMGAESIGLEDMAVEDKGEVVELLYQCRCGD 159

Query: 121 FFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSIDC 180
           +FFIDSGELEEMGYP+LR GSKVSLRTLDALPAS+VLPCGSCSLKVRLLIDS+S VSIDC
Sbjct: 160 YFFIDSGELEEMGYPMLRIGSKVSLRTLDALPASIVLPCGSCSLKVRLLIDSDSCVSIDC 219

[Close]

BLAST of Tan0005218 vs. ExPASy TrEMBL
Match: A0A6J1KK19 (DPH4 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111495973 PE=3 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 3.2e-85
Identity = 160/180 (88.89%), Postives = 170/180 (94.44%), Query Frame = 0


Query: 1   MSSSSGIIGETYYDVLSLREDASHDEIRANYRSALLNFHPDKLQAMCQRSHPDDIMGERY 60
           MSSSSG IGETYYDVLSLREDASHDEIRA+YRSALLNFHPDKLQAMCQ+SHP+D++GERY
Sbjct: 1   MSSSSGSIGETYYDVLSLREDASHDEIRASYRSALLNFHPDKLQAMCQKSHPNDVVGERY 60

Query: 61  FKVQKAWEVLGSSTSRAAYDRELQAAKGDAMDAESISLEDMVVEGKGEVAELFYQCRCGD 120
           FKVQKAWEVLGSS SRAAYDRELQAAKG+AM AESI LEDM VE KGEV EL YQCRCGD
Sbjct: 61  FKVQKAWEVLGSSMSRAAYDRELQAAKGEAMGAESIGLEDMAVEDKGEVVELLYQCRCGD 120

Query: 121 FFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSIDC 180
           +FFIDSGELEEMGYP+LR GSKVSLRTLDALPAS+VLPCGSCSLKVRLLIDS+S VSIDC
Sbjct: 121 YFFIDSGELEEMGYPMLRIGSKVSLRTLDALPASIVLPCGSCSLKVRLLIDSDSCVSIDC 180

[Close]

BLAST of Tan0005218 vs. ExPASy TrEMBL
Match: A0A6J1KMK1 (DPH4 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495973 PE=3 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 3.2e-85
Identity = 160/180 (88.89%), Postives = 170/180 (94.44%), Query Frame = 0


Query: 1   MSSSSGIIGETYYDVLSLREDASHDEIRANYRSALLNFHPDKLQAMCQRSHPDDIMGERY 60
           MSSSSG IGETYYDVLSLREDASHDEIRA+YRSALLNFHPDKLQAMCQ+SHP+D++GERY
Sbjct: 40  MSSSSGSIGETYYDVLSLREDASHDEIRASYRSALLNFHPDKLQAMCQKSHPNDVVGERY 99

Query: 61  FKVQKAWEVLGSSTSRAAYDRELQAAKGDAMDAESISLEDMVVEGKGEVAELFYQCRCGD 120
           FKVQKAWEVLGSS SRAAYDRELQAAKG+AM AESI LEDM VE KGEV EL YQCRCGD
Sbjct: 100 FKVQKAWEVLGSSMSRAAYDRELQAAKGEAMGAESIGLEDMAVEDKGEVVELLYQCRCGD 159

Query: 121 FFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSIDC 180
           +FFIDSGELEEMGYP+LR GSKVSLRTLDALPAS+VLPCGSCSLKVRLLIDS+S VSIDC
Sbjct: 160 YFFIDSGELEEMGYPMLRIGSKVSLRTLDALPASIVLPCGSCSLKVRLLIDSDSCVSIDC 219

[Close]

BLAST of Tan0005218 vs. ExPASy TrEMBL
Match: A0A1S3BBM0 (DPH4 homolog OS=Cucumis melo OX=3656 GN=LOC103487907 PE=3 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 4.1e-85
Identity = 162/180 (90.00%), Postives = 170/180 (94.44%), Query Frame = 0


Query: 1   MSSSSGIIGETYYDVLSLREDASHDEIRANYRSALLNFHPDKLQAMCQRSHPDDIMGERY 60
           MSSSSG IGETYYDVLSLREDAS DEIRA+YRSALLNFHPDKLQAMC++SHPDDI GERY
Sbjct: 1   MSSSSGAIGETYYDVLSLREDASFDEIRASYRSALLNFHPDKLQAMCRKSHPDDIKGERY 60

Query: 61  FKVQKAWEVLGSSTSRAAYDRELQAAKGDAMDAESISLEDMVVEGKGEVAELFYQCRCGD 120
           FKVQKAWEVLGS  SRA+YDRELQAAKGDA+ AESISLEDMVVE KGEV ELFYQCRCGD
Sbjct: 61  FKVQKAWEVLGSPKSRASYDRELQAAKGDAIGAESISLEDMVVEDKGEVVELFYQCRCGD 120

Query: 121 FFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSIDC 180
            FFIDSGEL+EMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKV L+ID+NSSVSIDC
Sbjct: 121 CFFIDSGELDEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVLLVIDTNSSVSIDC 180

[Close]

BLAST of Tan0005218 vs. ExPASy TrEMBL
Match: A0A6J1GGX6 (DPH4 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454088 PE=3 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 7.1e-85
Identity = 160/180 (88.89%), Postives = 167/180 (92.78%), Query Frame = 0


Query: 1   MSSSSGIIGETYYDVLSLREDASHDEIRANYRSALLNFHPDKLQAMCQRSHPDDIMGERY 60
           MSSSSG IGETYYDVLSLREDASHDEIRA+YRSALLNFHPDKLQAMCQ+SHPDD++GERY
Sbjct: 39  MSSSSGSIGETYYDVLSLREDASHDEIRASYRSALLNFHPDKLQAMCQKSHPDDVVGERY 98

Query: 61  FKVQKAWEVLGSSTSRAAYDRELQAAKGDAMDAESISLEDMVVEGKGEVAELFYQCRCGD 120
           FKVQKAWEVLGSS SRAAYDRELQAAKGDAM AESI LEDM VE KGEV ELFYQCRCGD
Sbjct: 99  FKVQKAWEVLGSSMSRAAYDRELQAAKGDAMGAESIGLEDMAVEDKGEVVELFYQCRCGD 158

Query: 121 FFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSIDC 180
           +FFIDS ELEEMGYP+LR GSKVSLRT DALPAS+VLPCGSCSLKVRLLIDS S  SIDC
Sbjct: 159 YFFIDSVELEEMGYPMLRIGSKVSLRTFDALPASIVLPCGSCSLKVRLLIDSGSCASIDC 218

[Close]

BLAST of Tan0005218 vs. ExPASy TrEMBL
Match: A0A6J1GHX3 (DPH4 homolog isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111454088 PE=3 SV=1)

HSP 1 Score: 323.2 bits (827), Expect = 7.1e-85
Identity = 160/180 (88.89%), Postives = 167/180 (92.78%), Query Frame = 0


Query: 1   MSSSSGIIGETYYDVLSLREDASHDEIRANYRSALLNFHPDKLQAMCQRSHPDDIMGERY 60
           MSSSSG IGETYYDVLSLREDASHDEIRA+YRSALLNFHPDKLQAMCQ+SHPDD++GERY
Sbjct: 1   MSSSSGSIGETYYDVLSLREDASHDEIRASYRSALLNFHPDKLQAMCQKSHPDDVVGERY 60

Query: 61  FKVQKAWEVLGSSTSRAAYDRELQAAKGDAMDAESISLEDMVVEGKGEVAELFYQCRCGD 120
           FKVQKAWEVLGSS SRAAYDRELQAAKGDAM AESI LEDM VE KGEV ELFYQCRCGD
Sbjct: 61  FKVQKAWEVLGSSMSRAAYDRELQAAKGDAMGAESIGLEDMAVEDKGEVVELFYQCRCGD 120

Query: 121 FFFIDSGELEEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSIDC 180
           +FFIDS ELEEMGYP+LR GSKVSLRT DALPAS+VLPCGSCSLKVRLLIDS S  SIDC
Sbjct: 121 YFFIDSVELEEMGYPMLRIGSKVSLRTFDALPASIVLPCGSCSLKVRLLIDSGSCASIDC 180

[Close]

BLAST of Tan0005218 vs. TAIR 10
Match: AT4G10130.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 184.1 bits (466), Expect = 9.8e-47
Identity = 91/171 (53.22%), Postives = 125/171 (73.10%), Query Frame = 0


Query: 8   IGETYYDVLSLREDASHDEIRANYRSALLNFHPDKLQAMCQRSHPDDIMGERYFKVQKAW 67
           + ETYY++LS++EDAS++EIR +YRSA+L+ HPDKL     RS  DD   E++ K+QKAW
Sbjct: 8   VHETYYEILSVKEDASYEEIRNSYRSAILHSHPDKLN-NTSRSSSDD---EKFLKIQKAW 67

Query: 68  EVLGSSTSRAAYDRELQAAKGDAMDAESISLEDMVVEGKGEVAELFYQCRCGDFFFIDSG 127
           EVL  +  R  YD +L++++ D + A+ IS+EDM VE  G+V +LFYQCRCGD+F +DS 
Sbjct: 68  EVLSDAELRVVYDNDLRSSRHDGITADEISIEDMSVEITGDVIDLFYQCRCGDYFCVDSS 127

Query: 128 ELEEMGYPLLRNGSKVSLRTLDALPASVVLPCGSCSLKVRLLIDSNSSVSI 179
           EL  MG+ LLR+G  V ++ L A  ASVVLPCGSCSLK R+ +DS+  + I
Sbjct: 128 ELGTMGFALLRDGDFVYVKRLGAFVASVVLPCGSCSLKTRVWVDSDMKIPI 174

[Close]

BLAST of Tan0005218 vs. TAIR 10
Match: AT1G74250.1 (DNAJ heat shock N-terminal domain-containing protein )

HSP 1 Score: 48.1 bits (113), Expect = 8.4e-06
Identity = 29/80 (36.25%), Postives = 44/80 (55.00%), Query Frame = 0


Query: 1  MSSSSGIIGETYYDVLSLREDASHDEIRANYRSALLNFHPDKLQAMCQRSHPDDIMGERY 60
          M+SSS      +Y+VL + +++S DEIR++YR   L  HPDKL      S  +     ++
Sbjct: 1  MASSSRSEKRCHYEVLGISKESSPDEIRSSYRRLALQRHPDKLMKAAGLSEAE--ATAQF 60

Query: 61 FKVQKAWEVLGSSTSRAAYD 81
           ++  A+EVL     RA YD
Sbjct: 61 QELVHAYEVLSDPKERAWYD 78

[Close]

BLAST of Tan0005218 vs. TAIR 10
Match: AT3G62600.1 (DNAJ heat shock family protein )

HSP 1 Score: 47.8 bits (112), Expect = 1.1e-05
Identity = 23/75 (30.67%), Postives = 41/75 (54.67%), Query Frame = 0


Query: 7  IIGETYYDVLSLREDASHDEIRANYRSALLNFHPDKLQAMCQRSHPDDIMGERYFKVQKA 66
          + G++YYDVL + + AS ++I+  YR   L +HPDK Q        ++    ++ ++  A
Sbjct: 22 LAGKSYYDVLQVPKGASDEQIKRAYRKLALKYHPDKNQG-------NEEATRKFAEINNA 81

Query: 67 WEVLGSSTSRAAYDR 82
          +EVL     R  Y++
Sbjct: 82 YEVLSDEEKREIYNK 89

[Close]

BLAST of Tan0005218 vs. TAIR 10
Match: AT4G37480.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 47.4 bits (111), Expect = 1.4e-05
Identity = 26/74 (35.14%), Postives = 40/74 (54.05%), Query Frame = 0


Query: 10  ETYYDVLSLREDASHDEIRANYRSALLNFHPDKLQAMCQRSHPDDIMGERYFKVQKAWEV 69
           E  YD+L++ E +S  EI+A++R      HPD ++     S  D     R+ ++  A+E+
Sbjct: 55  ENAYDILNVSETSSIAEIKASFRRLAKETHPDLIE-----SKKDPSNSRRFVQILAAYEI 114

Query: 70  LGSSTSRAAYDREL 84
           L  S  RA YDR L
Sbjct: 115 LSDSEKRAHYDRYL 123

[Close]

BLAST of Tan0005218 vs. TAIR 10
Match: AT1G56300.1 (Chaperone DnaJ-domain superfamily protein )

HSP 1 Score: 47.0 bits (110), Expect = 1.9e-05
Identity = 28/98 (28.57%), Postives = 49/98 (50.00%), Query Frame = 0


Query: 8   IGETYYDVLSLREDASHDEIRANYRSALLNFHPDKLQAMCQRSHPDDIMGE---RYFKVQ 67
           +  +YY +L +R+DAS  +IR  YR   + +HPD+       +    + GE   R+ ++Q
Sbjct: 10  VRSSYYTILGIRKDASVSDIRTAYRKLAMKWHPDRY------ARNPGVAGEAKRRFQQIQ 69

Query: 68  KAWEVLGSSTSRAAYDRELQAAKGDAMDAESISLEDMV 103
           +A+ VL     R+ YD  L     D  D     +++M+
Sbjct: 70  EAYSVLNDENKRSMYDVGLYDPHEDDDDDFCDFMQEMI 101

The following BLAST results are available for this feature:

BLAST of Tan0005218 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q54CI5	3.6e-09	27.61	DPH4 homolog OS=Dictyostelium discoideum OX=44689 GN=dph4 PE=3 SV=1	[more]
P47138	3.9e-08	32.91	Diphthamide biosynthesis protein 4 OS=Saccharomyces cerevisiae (strain ATCC 2045...	[more]
Q6FWM1	2.6e-07	29.52	Diphthamide biosynthesis protein 4 OS=Candida glabrata (strain ATCC 2001 / CBS 1...	[more]
B9KH92	4.4e-07	39.19	Chaperone protein DnaJ OS=Anaplasma marginale (strain Florida) OX=320483 GN=dnaJ...	[more]
Q5P9E0	4.4e-07	39.19	Chaperone protein DnaJ OS=Anaplasma marginale (strain St. Maries) OX=234826 GN=d...	[more]

BLAST of Tan0005218 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023523039.1	1.3e-85	88.89	DPH4 homolog [Cucurbita pepo subsp. pepo]	[more]
XP_023537718.1	2.9e-85	88.33	DPH4 homolog [Cucurbita pepo subsp. pepo]	[more]
XP_038884890.1	3.8e-85	91.06	DPH4 homolog isoform X2 [Benincasa hispida]	[more]
XP_023001946.1	6.5e-85	88.89	DPH4 homolog isoform X2 [Cucurbita maxima]	[more]
XP_023001945.1	6.5e-85	88.89	DPH4 homolog isoform X1 [Cucurbita maxima]	[more]

BLAST of Tan0005218 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KK19	3.2e-85	88.89	DPH4 homolog isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111495973 PE=3 SV=1	[more]
A0A6J1KMK1	3.2e-85	88.89	DPH4 homolog isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495973 PE=3 SV=1	[more]
A0A1S3BBM0	4.1e-85	90.00	DPH4 homolog OS=Cucumis melo OX=3656 GN=LOC103487907 PE=3 SV=1	[more]
A0A6J1GGX6	7.1e-85	88.89	DPH4 homolog isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454088 PE=3 SV=1	[more]
A0A6J1GHX3	7.1e-85	88.89	DPH4 homolog isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111454088 PE=3 SV=1	[more]

BLAST of Tan0005218 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G10130.1	9.8e-47	53.22	DNAJ heat shock N-terminal domain-containing protein	[more]
AT1G74250.1	8.4e-06	36.25	DNAJ heat shock N-terminal domain-containing protein	[more]
AT3G62600.1	1.1e-05	30.67	DNAJ heat shock family protein	[more]
AT4G37480.1	1.4e-05	35.14	Chaperone DnaJ-domain superfamily protein	[more]
AT1G56300.1	1.9e-05	28.57	Chaperone DnaJ-domain superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001623	DnaJ domain	PRINTS	PR00625	JDOMAIN	coord: 55..75 score: 22.36 coord: 13..31 score: 37.78 coord: 31..46 score: 34.71
IPR001623	DnaJ domain	SMART	SM00271	dnaj_3	coord: 10..75 e-value: 9.4E-10 score: 48.4
IPR001623	DnaJ domain	PFAM	PF00226	DnaJ	coord: 11..80 e-value: 8.5E-15 score: 54.6
IPR001623	DnaJ domain	PROSITE	PS50076	DNAJ_2	coord: 11..83 score: 14.575948
IPR001623	DnaJ domain	CDD	cd06257	DnaJ	coord: 11..71 e-value: 8.74968E-14 score: 60.6369
IPR036869	Chaperone J-domain superfamily	GENE3D	1.10.287.110	DnaJ domain	coord: 7..86 e-value: 2.4E-17 score: 64.9
IPR036869	Chaperone J-domain superfamily	SUPERFAMILY	46565	Chaperone J-domain	coord: 10..83
IPR036671	DPH-type metal-binding domain superfamily	GENE3D	3.10.660.10	DPH Zinc finger	coord: 87..179 e-value: 2.2E-15 score: 57.9
IPR036671	DPH-type metal-binding domain superfamily	SUPERFAMILY	144217	CSL zinc finger	coord: 93..172
IPR007872	DPH-type metal-binding domain	PFAM	PF05207	zf-CSL	coord: 95..169 e-value: 3.2E-12 score: 46.0
IPR007872	DPH-type metal-binding domain	PROSITE	PS51074	DPH_MB	coord: 92..171 score: 14.955948
IPR044248	Diphthamide biosynthesis protein 3/4-like	PANTHER	PTHR21454	DPH3 HOMOLOG-RELATED	coord: 1..172
None	No IPR available	PANTHER	PTHR21454:SF31	DIPHTHAMIDE BIOSYNTHESIS PROTEIN 4	coord: 1..172

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0005218.1	Tan0005218.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0017183	peptidyl-diphthamide biosynthetic process from peptidyl-histidine
biological_process	GO:0002098	tRNA wobble uridine modification
cellular_component	GO:0005829	cytosol
cellular_component	GO:0005634	nucleus
molecular_function	GO:0046872	metal ion binding