Homology
BLAST of Tan0005200 vs. ExPASy Swiss-Prot
Match:
Q9FK05 (Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3702 GN=PME61 PE=2 SV=1)
HSP 1 Score: 696.4 bits (1796), Expect = 2.8e-199
Identity = 369/593 (62.23%), Postives = 438/593 (73.86%), Query Frame = 0
Query: 28 GPSLP------PPLSSTSRRRTRILFISLLSILLITASAVSAAVLIRSHIGGATSSEAHF 87
GPS P L ++ L + L++L++ IR+ G T +
Sbjct: 10 GPSNPNQKDPATSLPELQKKTKTKLILFTLAVLVVGVVCFGIFAGIRAVDSGKTEPKL-- 69
Query: 88 PHNKPSQAISRACSLTLYPSLCLSSLLSFPGSLSADVHDLVHITLNLTLQHLNKALYSSS 147
KP+QAISR CS +LYP+LC+ +LL FPGSL+AD ++L+HI+ N TLQ +KALY+SS
Sbjct: 70 -TRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSS 129
Query: 148 QIPISQFSNNPLAHSAYEDCMELLNDAVDAFSLSLFSPAA-----SNHDVVTWLSAALTY 207
I +Q P SAY+ C+ELL+D+VDA + +L S S+ DV+TWLS+A+T
Sbjct: 130 TITYTQMP--PRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTN 189
Query: 208 HDTCMDGFADV---ADPVKDQMAAKLSDLSEMISNSLAIFSGFG---GGLPVQNRRRRRL 267
HDTC DGF ++ VKDQ+ + DLSEM+SN LAIF+G G+PV N R+
Sbjct: 190 HDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKL-- 249
Query: 268 MDSGSEWAAENGVTEGEFPEWVSGKDRRLLATPISAIQADIVVAKDGSGKFKTVAEAIAA 327
G+E TE E P W+ +DR LL TP SAIQADI V+KDGSG FKT+AEAI
Sbjct: 250 --LGTE------ETE-ELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKK 309
Query: 328 APSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIFDKVTTFRTA 387
AP S RR VIY+KAG+YEEENLKVGRKKTNLMF+GDGKG TVI+GGKSI D +TTF TA
Sbjct: 310 APEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTA 369
Query: 388 TFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFF 447
TFA +G G I+RDMTFEN AGP+KHQAVALR+ DHAVVY CNIIGYQD LYVHSNRQFF
Sbjct: 370 TFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFF 429
Query: 448 RECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKDPNQNTGISIHACKIL 507
REC+IYGTVDFIFGNA V+ QSCNIYARKPM QQK TITAQ+RKDPNQNTGISIHACK+L
Sbjct: 430 RECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLL 489
Query: 508 ATADLQPSKSSFPTFLGRPWKLYSRVVYMVSSIGDHIHPRGWLEWQGSFALDTLYYGEYM 567
AT DL+ SK S+PT+LGRPWKLYSRVVYM+S +GDHI PRGWLEW G FALD+LYYGEYM
Sbjct: 490 ATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYM 549
Query: 568 NSGPGAAVGQRVKWPGYRVITSTLEATKFTVGQFIYGSSWLPSTGVSFSAGLS 604
N G G+ +GQRVKWPGY VITST+EA+KFTV QFI GSSWLPSTGVSF +GLS
Sbjct: 550 NKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586
BLAST of Tan0005200 vs. ExPASy Swiss-Prot
Match:
Q9M3B0 (Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3702 GN=PME34 PE=2 SV=1)
HSP 1 Score: 682.2 bits (1759), Expect = 5.4e-195
Identity = 361/606 (59.57%), Postives = 444/606 (73.27%), Query Frame = 0
Query: 9 SSVSGDTPTTSELSHDPNRGPSLPPPLSSTSRRRTRILFISL-LSILLITASAVSAAVLI 68
S +G T++ + N+ + P ++ R + +++ S+ L+I LI A+A+ A V
Sbjct: 9 SGATGSVTTSTTTAPILNQVSTSEQPENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRS 68
Query: 69 RSHIGGATSSEAHFPHNKPSQAISRACSLTLYPSLCLSSLLSFPGSLSA-DVHDLVHITL 128
R + + A KPSQAIS+AC LT +P LC+ SL+ FPGSL+A DL+H+T+
Sbjct: 69 RLKLNQSVPGLA----RKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTV 128
Query: 129 NLTLQHLNKALYSSSQIPISQFSNNPLAHSAYEDCMELLNDAVDAFSLSLFSPAASN--- 188
N+TL H + ALYSS+ +S P A SAY+ C+ELL+D+VDA S +L S +S+
Sbjct: 129 NMTLHHFSHALYSSAS--LSFVDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSSAKP 188
Query: 189 HDVVTWLSAALTYHDTCMDGFADVAD-PVKDQMAAKLSDLSEMISNSLAIFSGFG----- 248
DV TWLSAALT HDTC +GF V D VKD M A L +LSE++SN LAIFS
Sbjct: 189 QDVTTWLSAALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDF 248
Query: 249 GGLPVQNRRRRRLMDSGSEWAAENGVTEGEFPEWVSGKDRRLLATPISAIQADIVVAKDG 308
G+P+QNRR + + E +FP W+ K+R +L P+S IQADI+V+KDG
Sbjct: 249 AGVPIQNRRLLGVEE-----------REEKFPRWMRPKEREILEMPVSQIQADIIVSKDG 308
Query: 309 SGKFKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGG 368
+G KT++EAI AP +S RRI+IY+KAG+YEE NLKVGRKK NLMFVGDGKG TVISGG
Sbjct: 309 NGTCKTISEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGG 368
Query: 369 KSIFDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGY 428
KSIFD +TTF TA+FA +G G I RD+TFEN AGP+KHQAVALR+ ADHAV+Y CNIIGY
Sbjct: 369 KSIFDNITTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGY 428
Query: 429 QDTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKDPN 488
QDTLYVHSNRQFFRECDIYGTVDFIFGNA VV Q+C+IYARKPM QKNTITAQ+RKDPN
Sbjct: 429 QDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPN 488
Query: 489 QNTGISIHACKILATADLQPSKSSFPTFLGRPWKLYSRVVYMVSSIGDHIHPRGWLEWQG 548
QNTGISIHA ++LA +DLQ + S T+LGRPWKL+SR VYM+S IG H+H RGWLEW
Sbjct: 489 QNTGISIHASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNT 548
Query: 549 SFALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTLEATKFTVGQFIYGSSWLPSTGVS 604
+FALDTLYYGEY+NSGPG+ +GQRV WPGYRVI ST EA +FTV +FIYGSSWLPSTGVS
Sbjct: 549 TFALDTLYYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVS 597
BLAST of Tan0005200 vs. ExPASy Swiss-Prot
Match:
O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)
HSP 1 Score: 447.2 bits (1149), Expect = 2.9e-124
Identity = 255/576 (44.27%), Postives = 354/576 (61.46%), Query Frame = 0
Query: 41 RRTRILFISLLSILLITASAVSAAVLIRSHIGGATSSEAHFPHNKPSQAISR-ACSLTLY 100
++ + LF++L + LL+ A+ + + S + N+P AI + +CS T Y
Sbjct: 25 KKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNG------NEPHHAILKSSCSSTRY 84
Query: 101 PSLCLSSLLSFPGSLS--ADVHDLVHITLNLTLQHLNKALYSSSQIPISQFSNNPLAHSA 160
P LC S++ + P + D++ ++LN+T + + ++ + + + A
Sbjct: 85 PDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKL-LKRTNLTKREKVA 144
Query: 161 YEDCMELLNDAVDAFSLSL-------FSPAASNH--DVVTWLSAALTYHDTCMDGFA--D 220
DC+E +++ +D ++ + S H D+ T +SAA+T TC+DGF+ D
Sbjct: 145 LHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDD 204
Query: 221 VADPVKDQMAAKLSDLSEMISNSLAIFSGFGGGLPVQNRRRRRLMDSGSEWAAENGVTEG 280
V+D ++ + +M SN+LA+ + + + S + E T
Sbjct: 205 ANKHVRDALSDGQVHVEKMCSNALAMIKN------MTDTDMMIMRTSNNRKLTEETSTVD 264
Query: 281 EFPEWVSGKDRRLLATPISAIQADIVVAKDGSGKFKTVAEAIAAAPSSSGRRIVIYIKAG 340
+P W+S DRRLL + S++ + VVA DGSG FKTVA A+AAAP +R +I IKAG
Sbjct: 265 GWPAWLSPGDRRLLQS--SSVTPNAVVAADGSGNFKTVAAAVAAAPQGGTKRYIIRIKAG 324
Query: 341 KYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIFDKVTTFRTATFAGSGTGIILRDMTF 400
Y EN++V +K N+MF+GDG+ T+I+G +++ D TTF++AT A G G + RD+TF
Sbjct: 325 VY-RENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAVVGEGFLARDITF 384
Query: 401 ENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNA 460
+NTAGPSKHQAVALR+ AD + Y+C+++ YQDTLYVHSNRQFF C I GTVDFIFGNA
Sbjct: 385 QNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFGNA 444
Query: 461 VVVFQSCNIYARKPMPQQKNTITAQDRKDPNQNTGISIHACKILATADLQPSKSSFPTFL 520
V Q+C+I+ARKP QKN +TAQ R DPNQNTGI I +I AT+DL+P + SFPT+L
Sbjct: 445 AAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQGSFPTYL 504
Query: 521 GRPWKLYSRVVYMVSSIGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRVKWPG 580
GRPWK YSR V M SSI D IHP GW EW G+FAL+TL+YGE+ NSG GA RVKW G
Sbjct: 505 GRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTSGRVKWKG 564
Query: 581 YRVITSTLEATKFTVGQFIYGSSWLPSTGVSFSAGL 603
+RVITS EA FT G FI GSSWL STG FS GL
Sbjct: 565 FRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584
BLAST of Tan0005200 vs. ExPASy Swiss-Prot
Match:
P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)
HSP 1 Score: 447.2 bits (1149), Expect = 2.9e-124
Identity = 254/578 (43.94%), Postives = 358/578 (61.94%), Query Frame = 0
Query: 41 RRTRILFISLLSILLITASAVSAAVLIRSHIGGATSSEAHFPHNKPSQAISR-ACSLTLY 100
++ + LF++L + LL+ A+ + + S + N+P AI + +CS T Y
Sbjct: 25 KKKKKLFLALFATLLVVAAVIGIVAGVNSRKNSGDNG------NEPHHAILKSSCSSTRY 84
Query: 101 PSLCLSSLLSFPGSLS--ADVHDLVHITLNLTLQHLNKALYSSSQIPISQFSNNPLAHSA 160
P LC S++ + P + D++ ++LN+T + + ++ + + + A
Sbjct: 85 PDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKL-LKRTNLTKREKVA 144
Query: 161 YEDCMELLNDAVDAFSLSL-------FSPAASNH--DVVTWLSAALTYHDTCMDGFA--D 220
DC+E +++ +D ++ + S H D+ T +SAA+T TC+DGF+ D
Sbjct: 145 LHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTCLDGFSHDD 204
Query: 221 VADPVKDQMAAKLSDLSEMISNSLAIFSGFGGG--LPVQNRRRRRLMDSGSEWAAENGVT 280
V+D ++ + +M SN+LA+ + ++ R+L++ S T
Sbjct: 205 ANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSNNRKLIEETS--------T 264
Query: 281 EGEFPEWVSGKDRRLLATPISAIQADIVVAKDGSGKFKTVAEAIAAAPSSSGRRIVIYIK 340
+P W+S DRRLL + S++ ++VVA DGSG FKTVA ++AAAP +R +I IK
Sbjct: 265 VDGWPAWLSTGDRRLLQS--SSVTPNVVVAADGSGNFKTVAASVAAAPQGGTKRYIIRIK 324
Query: 341 AGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIFDKVTTFRTATFAGSGTGIILRDM 400
AG Y EN++V +K N+MF+GDG+ T+I+G +++ D TTF++AT A G G + RD+
Sbjct: 325 AGVY-RENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGFLARDI 384
Query: 401 TFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFG 460
TF+NTAGPSKHQAVALR+ AD + Y+C+++ YQDTLYVHSNRQFF C I GTVDFIFG
Sbjct: 385 TFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTVDFIFG 444
Query: 461 NAVVVFQSCNIYARKPMPQQKNTITAQDRKDPNQNTGISIHACKILATADLQPSKSSFPT 520
NA V Q+C+I+ARKP QKN +TAQ R DPNQNTGI I +I AT+DL+P + SFPT
Sbjct: 445 NAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQGSFPT 504
Query: 521 FLGRPWKLYSRVVYMVSSIGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRVKW 580
+LGRPWK YSR V M SSI D IHP GW EW G+FAL+TL+YGE+ N+G GA RVKW
Sbjct: 505 YLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTSGRVKW 564
Query: 581 PGYRVITSTLEATKFTVGQFIYGSSWLPSTGVSFSAGL 603
G+RVITS EA FT G FI GSSWL STG FS GL
Sbjct: 565 KGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584
BLAST of Tan0005200 vs. ExPASy Swiss-Prot
Match:
Q43143 (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PMEU1 PE=2 SV=1)
HSP 1 Score: 445.7 bits (1145), Expect = 8.6e-124
Identity = 252/584 (43.15%), Postives = 362/584 (61.99%), Query Frame = 0
Query: 41 RRTRILFISLLSILLITASAVSAAVLIRSHIGG--------ATSSEAHFPHNKPSQAISR 100
+R + +++++++ +L+ A+ + ++SH A SS AH +
Sbjct: 16 KRKKKIYLAIVASVLLVAAVIGVVAGVKSHSKNSDDHADIMAISSSAH-------AIVKS 75
Query: 101 ACSLTLYPSLCLSSLLS---FPGSLSADVHDLVHITLNLTLQHLNKALYSSSQIPISQFS 160
ACS TL+P LC S++++ F +++ D++ ++LN+T++ + + Y+ ++ ++
Sbjct: 76 ACSNTLHPELCYSAIVNVSDFSKKVTSQ-KDVIELSLNITVKAVRRNYYAVKELIKTRKG 135
Query: 161 NNPLAHSAYEDCMELLNDAVDAF-----SLSLFSPAAS----NHDVVTWLSAALTYHDTC 220
P A DC+E +++ +D L L+ S D+ T +S+A+T +TC
Sbjct: 136 LTPREKVALHDCLETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLISSAITNQETC 195
Query: 221 MDGFA-DVAD-PVKDQMAAKLSDLSEMISNSLAIFSGFGGGLPVQNRRRRRLMDSGSEWA 280
+DGF+ D AD V+ + + +M SN+LA+ + N + + +
Sbjct: 196 LDGFSHDEADKKVRKVLLKGQKHVEKMCSNALAMICNM-TDTDIANEMKLSAPANNRKLV 255
Query: 281 AENGVTEGEFPEWVSGKDRRLLATPISAIQADIVVAKDGSGKFKTVAEAIAAAPSSSGRR 340
+N GE+PEW+S DRRLL + S + D+VVA DGSG +KTV+EA+ AP S +R
Sbjct: 256 EDN----GEWPEWLSAGDRRLLQS--STVTPDVVVAADGSGDYKTVSEAVRKAPEKSSKR 315
Query: 341 IVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIFDKVTTFRTATFAGSGTG 400
VI IKAG Y EN+ V +KKTN+MF+GDGK T+I+ +++ D TTF +AT
Sbjct: 316 YVIRIKAGVY-RENVDVPKKKTNIMFMGDGKSNTIITASRNVQDGSTTFHSATVVRVAGK 375
Query: 401 IILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDIYGT 460
++ RD+TF+NTAG SKHQAVAL + +D + Y C+++ YQDTLYVHSNRQFF +C + GT
Sbjct: 376 VLARDITFQNTAGASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVHSNRQFFVQCLVAGT 435
Query: 461 VDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKDPNQNTGISIHACKILATADLQPS 520
VDFIFGN VFQ C+I+AR+P QKN +TAQ R DPNQNTGI I C+I AT+DL+P
Sbjct: 436 VDFIFGNGAAVFQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGATSDLRPV 495
Query: 521 KSSFPTFLGRPWKLYSRVVYMVSSIGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAV 580
+ SFPT+LGRPWK YSR V M SSI D I P GW EW G+FALDTL+YGEY N+G GA
Sbjct: 496 QKSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDTLFYGEYANTGAGAPT 555
Query: 581 GQRVKWPGYRVITSTLEATKFTVGQFIYGSSWLPSTGVSFSAGL 603
RVKW G++VITS+ EA +T G+FI G SWL STG FS GL
Sbjct: 556 SGRVKWKGHKVITSSTEAQAYTPGRFIAGGSWLSSTGFPFSLGL 583
BLAST of Tan0005200 vs. NCBI nr
Match:
XP_022957644.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita moschata])
HSP 1 Score: 1046.6 bits (2705), Expect = 8.4e-302
Identity = 534/607 (87.97%), Postives = 558/607 (91.93%), Query Frame = 0
Query: 1 MNNYNKLHSSVSGDTPTTSELSHDPNRGPSLPPPLSSTSRRRTRILFISLLSILLITASA 60
MNNYNKLHSS SGDTP T ++SH PN PP SS SRRRTRILF+SL+SILLITASA
Sbjct: 1 MNNYNKLHSSASGDTPATFQVSHTPN------PPSSSASRRRTRILFVSLISILLITASA 60
Query: 61 VSAAVLIRSHIGGATSSEAHFPHNKPSQAISRACSLTLYPSLCLSSLLSFPGSLSADVHD 120
VSAAVL+ S +HFPHNKP+QAIS ACS TLYPSLC SSLLSFPGSLSADVH
Sbjct: 61 VSAAVLLH-------SQSSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHR 120
Query: 121 LVHITLNLTLQHLNKALYSSSQIPISQFSNNPLAHSAYEDCMELLNDAVDAFSLSLFSPA 180
LVHI+LNLTLQHL KALY+SSQIP+ Q SNNPLAHSAY+DC+ELLNDAVDAFSLSLFS +
Sbjct: 121 LVHISLNLTLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTS 180
Query: 181 ASNHDVVTWLSAALTYHDTCMDGFADVADPVKDQMAAKLSDLSEMISNSLAIFSGFGGGL 240
ASNHD++TWLSAALTYHDTC DGFADV DPVKDQMAA+LSDLSEMISNSLAIFSG+G GL
Sbjct: 181 ASNHDIMTWLSAALTYHDTCSDGFADVDDPVKDQMAARLSDLSEMISNSLAIFSGYGEGL 240
Query: 241 PVQNRRRRRLMDSGSEWAAENG--VTEGEFPEWVSGKDRRLLATPISAIQADIVVAKDGS 300
PVQN RRRLM+S EWAAENG V EGEFP WVSGKDRRLLA PISAIQADIVVAKDGS
Sbjct: 241 PVQN--RRRLMESDREWAAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGS 300
Query: 301 GKFKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 360
GKFKTVAEAI AAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK
Sbjct: 301 GKFKTVAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 360
Query: 361 SIFDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQ 420
S+FDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALR+SADHAVVY CNIIGYQ
Sbjct: 361 SVFDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRVSADHAVVYRCNIIGYQ 420
Query: 421 DTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKDPNQ 480
DTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQ+RKDPNQ
Sbjct: 421 DTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQ 480
Query: 481 NTGISIHACKILATADLQPSKSSFPTFLGRPWKLYSRVVYMVSSIGDHIHPRGWLEWQGS 540
NTGISIHACKILAT DL+ SK SFPTFLGRPWKLYSRVVYMVSS+GDHIHPRGWLEWQGS
Sbjct: 481 NTGISIHACKILATTDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGS 540
Query: 541 FALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTLEATKFTVGQFIYGSSWLPSTGVSF 600
FALDTLYYGEYMNSGPGAA GQRV WPGYRVITST+EATKFTVGQFIYGSSWLPSTGVSF
Sbjct: 541 FALDTLYYGEYMNSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSF 592
Query: 601 SAGLSAN 606
SAGLSAN
Sbjct: 601 SAGLSAN 592
BLAST of Tan0005200 vs. NCBI nr
Match:
XP_023533011.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1043.5 bits (2697), Expect = 7.1e-301
Identity = 533/607 (87.81%), Postives = 558/607 (91.93%), Query Frame = 0
Query: 1 MNNYNKLHSSVSGDTPTTSELSHDPNRGPSLPPPLSSTSRRRTRILFISLLSILLITASA 60
MNNYNKLHSS SGDTP T ++SH PN PP SS SRRRTRILF+SL+SILLITASA
Sbjct: 1 MNNYNKLHSSGSGDTPATFQVSHTPN------PPSSSASRRRTRILFVSLISILLITASA 60
Query: 61 VSAAVLIRSHIGGATSSEAHFPHNKPSQAISRACSLTLYPSLCLSSLLSFPGSLSADVHD 120
VSAAVLI ++ +HFPHNKP+QAIS ACS TLYPSLC SSLLSFPGSLSADVH
Sbjct: 61 VSAAVLIH-------TNTSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHH 120
Query: 121 LVHITLNLTLQHLNKALYSSSQIPISQFSNNPLAHSAYEDCMELLNDAVDAFSLSLFSPA 180
LVHI+LNLTLQHL KALY+SSQIP+ Q SNNPLAHSAY+DC+ELLNDAVDAFSLSLFS +
Sbjct: 121 LVHISLNLTLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTS 180
Query: 181 ASNHDVVTWLSAALTYHDTCMDGFADVADPVKDQMAAKLSDLSEMISNSLAIFSGFGGGL 240
ASNHD++TWLSAALTYHDTC DGFADV DPVKDQMAA+LSDLSEMISNSLAIFSG+G GL
Sbjct: 181 ASNHDIMTWLSAALTYHDTCSDGFADVDDPVKDQMAARLSDLSEMISNSLAIFSGYGQGL 240
Query: 241 PVQNRRRRRLMDSGSEWAAENG--VTEGEFPEWVSGKDRRLLATPISAIQADIVVAKDGS 300
PVQN RRRLM+S SEWAAENG V EGEFP WVSGKDRRLLA PISAIQADIVVAKDGS
Sbjct: 241 PVQN--RRRLMESDSEWAAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGS 300
Query: 301 GKFKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 360
GKF TVAEAI AAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK
Sbjct: 301 GKFTTVAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 360
Query: 361 SIFDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQ 420
S+FDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALR+SADHAVVY CNIIGYQ
Sbjct: 361 SVFDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRVSADHAVVYRCNIIGYQ 420
Query: 421 DTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKDPNQ 480
DTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQ+RKDPNQ
Sbjct: 421 DTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQ 480
Query: 481 NTGISIHACKILATADLQPSKSSFPTFLGRPWKLYSRVVYMVSSIGDHIHPRGWLEWQGS 540
NTGISIHACKILAT DL+ SK SFPTFLGRPWKLYSRVVYMVSS+GDHIHPRGWLEWQGS
Sbjct: 481 NTGISIHACKILATTDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGS 540
Query: 541 FALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTLEATKFTVGQFIYGSSWLPSTGVSF 600
FALDTLYYGEYMNSGPGAA GQRV WPGYRVITST+EATKFTVGQFIYGSSWLPSTGVSF
Sbjct: 541 FALDTLYYGEYMNSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSF 592
Query: 601 SAGLSAN 606
SAGLS N
Sbjct: 601 SAGLSTN 592
BLAST of Tan0005200 vs. NCBI nr
Match:
KAG6605784.1 (putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1038.5 bits (2684), Expect = 2.3e-299
Identity = 530/607 (87.31%), Postives = 553/607 (91.10%), Query Frame = 0
Query: 1 MNNYNKLHSSVSGDTPTTSELSHDPNRGPSLPPPLSSTSRRRTRILFISLLSILLITASA 60
MNNYNKLHSS SGDTP T ++SH PN PP SS SRRRTRILF+SL+SILLI SA
Sbjct: 1 MNNYNKLHSSASGDTPATFQVSHTPN------PPSSSASRRRTRILFVSLISILLIAVSA 60
Query: 61 VSAAVLIRSHIGGATSSEAHFPHNKPSQAISRACSLTLYPSLCLSSLLSFPGSLSADVHD 120
VSAAVLI SH +HFPHNKP+QAIS ACS TLYPSLC SSLLSFPGSLSADVH
Sbjct: 61 VSAAVLIHSH-------TSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHR 120
Query: 121 LVHITLNLTLQHLNKALYSSSQIPISQFSNNPLAHSAYEDCMELLNDAVDAFSLSLFSPA 180
LVHI+LNLTLQHL KALY+SSQIP+ Q SNNPLAHSAY+DC+ELLNDAVDAFSLSLFS +
Sbjct: 121 LVHISLNLTLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTS 180
Query: 181 ASNHDVVTWLSAALTYHDTCMDGFADVADPVKDQMAAKLSDLSEMISNSLAIFSGFGGGL 240
ASNHD++TWLSAALTYHDTC DGFADV DPVKDQMAA+LSDLSEMISNSLAIFSG+G L
Sbjct: 181 ASNHDIMTWLSAALTYHDTCSDGFADVDDPVKDQMAARLSDLSEMISNSLAIFSGYGEEL 240
Query: 241 PVQNRRRRRLMDSGSEWAAENG--VTEGEFPEWVSGKDRRLLATPISAIQADIVVAKDGS 300
PVQN RRRLM+S EW AENG V EGEFP WVSGKDRRLLA PISAIQADIVVAKDGS
Sbjct: 241 PVQN--RRRLMESDREWVAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGS 300
Query: 301 GKFKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 360
GKF TVAEAI AAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK
Sbjct: 301 GKFTTVAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 360
Query: 361 SIFDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQ 420
S+FDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALR+SADHAVVY CNIIGYQ
Sbjct: 361 SVFDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRVSADHAVVYRCNIIGYQ 420
Query: 421 DTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKDPNQ 480
DTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQ+RKDPNQ
Sbjct: 421 DTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQ 480
Query: 481 NTGISIHACKILATADLQPSKSSFPTFLGRPWKLYSRVVYMVSSIGDHIHPRGWLEWQGS 540
NTGISIHACKILAT DL+ SK SFPTFLGRPWKLYSRVVYMVSS+GDHIHPRGWLEWQGS
Sbjct: 481 NTGISIHACKILATTDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGS 540
Query: 541 FALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTLEATKFTVGQFIYGSSWLPSTGVSF 600
FALDTLYYGEYMNSGPGAA GQRV WPGYRVITST+EATKFTVGQFIYGSSWLPSTGVSF
Sbjct: 541 FALDTLYYGEYMNSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSF 592
Query: 601 SAGLSAN 606
SAGLS N
Sbjct: 601 SAGLSGN 592
BLAST of Tan0005200 vs. NCBI nr
Match:
XP_022996280.1 (probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita maxima])
HSP 1 Score: 1035.4 bits (2676), Expect = 1.9e-298
Identity = 530/607 (87.31%), Postives = 554/607 (91.27%), Query Frame = 0
Query: 1 MNNYNKLHSSVSGDTPTTSELSHDPNRGPSLPPPLSSTSRRRTRILFISLLSILLITASA 60
MNNYNKLHSS SGDTP T ++SH PN PP SS SRRRTRILF+SL+SILLITASA
Sbjct: 1 MNNYNKLHSSGSGDTPATFQVSHTPN---PPHPPSSSASRRRTRILFVSLISILLITASA 60
Query: 61 VSAAVLIRSHIGGATSSEAHFPHNKPSQAISRACSLTLYPSLCLSSLLSFPGSLSADVHD 120
VSAAVLI SH +H PHNKP+QAIS ACS TLYPSLC SSLLSFPGSLSADVH
Sbjct: 61 VSAAVLIHSH-------SSHIPHNKPTQAISHACSRTLYPSLCRSSLLSFPGSLSADVHH 120
Query: 121 LVHITLNLTLQHLNKALYSSSQIPISQFSNNPLAHSAYEDCMELLNDAVDAFSLSLFSPA 180
LVHI+LNLTLQHL KALY+SSQIP+ Q SNNPLAHSAY+DC+ELLNDAVDAFSLSLFS +
Sbjct: 121 LVHISLNLTLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTS 180
Query: 181 ASNHDVVTWLSAALTYHDTCMDGFADVADPVKDQMAAKLSDLSEMISNSLAIFSGFGGGL 240
ASNHD++TWLSAALTYHDTC DGFADV DPVKDQMA++LSDLSEMISNSLAIFSG+G GL
Sbjct: 181 ASNHDIMTWLSAALTYHDTCSDGFADVDDPVKDQMASRLSDLSEMISNSLAIFSGYGEGL 240
Query: 241 PVQNRRRRRLMDSGSEWAAENG--VTEGEFPEWVSGKDRRLLATPISAIQADIVVAKDGS 300
PVQN RRRLM+S EWAAENG V EGEFP WVSGKDRRLLA ISAIQADIVVAKDGS
Sbjct: 241 PVQN--RRRLMESDREWAAENGVAVAEGEFPGWVSGKDRRLLAASISAIQADIVVAKDGS 300
Query: 301 GKFKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 360
GKFKTVAEAI AAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK
Sbjct: 301 GKFKTVAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 360
Query: 361 SIFDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQ 420
S+FDKVTTFRTATFAGSGTGIILRDMT ENTAGPSKHQAVALR+SADHAVVY CNIIGYQ
Sbjct: 361 SVFDKVTTFRTATFAGSGTGIILRDMTIENTAGPSKHQAVALRVSADHAVVYRCNIIGYQ 420
Query: 421 DTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKDPNQ 480
DTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQ+RKDPNQ
Sbjct: 421 DTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQ 480
Query: 481 NTGISIHACKILATADLQPSKSSFPTFLGRPWKLYSRVVYMVSSIGDHIHPRGWLEWQGS 540
NTGISIHACKILAT DL+ SK SF TFLGRPWKLYSRVVYMVSS+GDHIHPRGWLEWQGS
Sbjct: 481 NTGISIHACKILATTDLEASKGSFSTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGS 540
Query: 541 FALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTLEATKFTVGQFIYGSSWLPSTGVSF 600
FALDTLYYGEYMNSGPGAA GQRV WPGYRVITST+EATKFTVGQFIYGSSWLPSTGVSF
Sbjct: 541 FALDTLYYGEYMNSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSF 595
Query: 601 SAGLSAN 606
SAGLS N
Sbjct: 601 SAGLSGN 595
BLAST of Tan0005200 vs. NCBI nr
Match:
KAG6579321.1 (putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1018.8 bits (2633), Expect = 1.9e-293
Identity = 519/605 (85.79%), Postives = 554/605 (91.57%), Query Frame = 0
Query: 2 NNYNKLHSSVSGDTPTTSELSHDPNRGPSLPPPLSSTSRRRTRILFISLLSILLITASAV 61
NNYNKLHSS SGDTP +S + + P LPPP S+SRR RIL IS LSI LITASAV
Sbjct: 3 NNYNKLHSSRSGDTPASSFRVSNDHSDP-LPPPSPSSSRR--RILLISFLSIFLITASAV 62
Query: 62 SAAVLIRSHIGGATSSEAHFPHNKPSQAISRACSLTLYPSLCLSSLLSFPGSLSADVHDL 121
SA VL+ S I A S+E+HFPHNKP+QAISRACSLTLYPSLCLSSLLS PGSLSADVHDL
Sbjct: 63 SAVVLVHSRI--AASTESHFPHNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDL 122
Query: 122 VHITLNLTLQHLNKALYSSSQIPISQFSNNPLAHSAYEDCMELLNDAVDAFSLSLFSPAA 181
VHI+LNLTLQHL KALYS+SQIPISQ SN+PLA SAYEDCMELLNDAV+AFSLSLFS A
Sbjct: 123 VHISLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTA 182
Query: 182 SNHDVVTWLSAALTYHDTCMDGFADVADPVKDQMAAKLSDLSEMISNSLAIFSGFGG-GL 241
+NHDV+TWLSAALTYHDTC DGF+DVADP+KDQ+ AKLSDLSEMISNSLAIFSGFGG L
Sbjct: 183 TNHDVMTWLSAALTYHDTCTDGFSDVADPIKDQITAKLSDLSEMISNSLAIFSGFGGDDL 242
Query: 242 PVQNRRRRRLMDSGSEWAAENGVTEGEFPEWVSGKDRRLLATPISAIQADIVVAKDGSGK 301
PV+NRRRRRLM+S W AE +G+FP+WVSGKDRRLLA PIS+IQADIVVAKDGSGK
Sbjct: 243 PVENRRRRRLMESDEHWLAEENNGDGDFPQWVSGKDRRLLAAPISSIQADIVVAKDGSGK 302
Query: 302 FKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSI 361
FKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKS+
Sbjct: 303 FKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV 362
Query: 362 FDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDT 421
+DKVTTFRTATFAGSGTG+ILRDMTFENTAGP+KHQAVALRLSADHAVV+ N+IGYQDT
Sbjct: 363 YDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVIGYQDT 422
Query: 422 LYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKDPNQNT 481
LYVHSNRQFFRECDIYGT+DFIFGNA VVFQSCNIYARKPM QKNTITAQDRKDPNQNT
Sbjct: 423 LYVHSNRQFFRECDIYGTIDFIFGNAAVVFQSCNIYARKPMSGQKNTITAQDRKDPNQNT 482
Query: 482 GISIHACKILATADLQPSKSSFPTFLGRPWKLYSRVVYMVSSIGDHIHPRGWLEWQGSFA 541
GISIHACKI+AT DL+ SK SFPT+LGRPWKLYSRVVYMVSS+GDHIHPRGWLEWQGSFA
Sbjct: 483 GISIHACKIIATGDLEASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 542
Query: 542 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTLEATKFTVGQFIYGSSWLPSTGVSFSA 601
LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITST+EATKFTVGQFIYGSSWLPSTGVSFSA
Sbjct: 543 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSFSA 602
Query: 602 GLSAN 606
GLS N
Sbjct: 603 GLSTN 602
BLAST of Tan0005200 vs. ExPASy TrEMBL
Match:
A0A6J1H2K1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459120 PE=3 SV=1)
HSP 1 Score: 1046.6 bits (2705), Expect = 4.1e-302
Identity = 534/607 (87.97%), Postives = 558/607 (91.93%), Query Frame = 0
Query: 1 MNNYNKLHSSVSGDTPTTSELSHDPNRGPSLPPPLSSTSRRRTRILFISLLSILLITASA 60
MNNYNKLHSS SGDTP T ++SH PN PP SS SRRRTRILF+SL+SILLITASA
Sbjct: 1 MNNYNKLHSSASGDTPATFQVSHTPN------PPSSSASRRRTRILFVSLISILLITASA 60
Query: 61 VSAAVLIRSHIGGATSSEAHFPHNKPSQAISRACSLTLYPSLCLSSLLSFPGSLSADVHD 120
VSAAVL+ S +HFPHNKP+QAIS ACS TLYPSLC SSLLSFPGSLSADVH
Sbjct: 61 VSAAVLLH-------SQSSHFPHNKPTQAISHACSHTLYPSLCRSSLLSFPGSLSADVHR 120
Query: 121 LVHITLNLTLQHLNKALYSSSQIPISQFSNNPLAHSAYEDCMELLNDAVDAFSLSLFSPA 180
LVHI+LNLTLQHL KALY+SSQIP+ Q SNNPLAHSAY+DC+ELLNDAVDAFSLSLFS +
Sbjct: 121 LVHISLNLTLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTS 180
Query: 181 ASNHDVVTWLSAALTYHDTCMDGFADVADPVKDQMAAKLSDLSEMISNSLAIFSGFGGGL 240
ASNHD++TWLSAALTYHDTC DGFADV DPVKDQMAA+LSDLSEMISNSLAIFSG+G GL
Sbjct: 181 ASNHDIMTWLSAALTYHDTCSDGFADVDDPVKDQMAARLSDLSEMISNSLAIFSGYGEGL 240
Query: 241 PVQNRRRRRLMDSGSEWAAENG--VTEGEFPEWVSGKDRRLLATPISAIQADIVVAKDGS 300
PVQN RRRLM+S EWAAENG V EGEFP WVSGKDRRLLA PISAIQADIVVAKDGS
Sbjct: 241 PVQN--RRRLMESDREWAAENGVAVAEGEFPGWVSGKDRRLLAAPISAIQADIVVAKDGS 300
Query: 301 GKFKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 360
GKFKTVAEAI AAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK
Sbjct: 301 GKFKTVAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 360
Query: 361 SIFDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQ 420
S+FDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALR+SADHAVVY CNIIGYQ
Sbjct: 361 SVFDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRVSADHAVVYRCNIIGYQ 420
Query: 421 DTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKDPNQ 480
DTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQ+RKDPNQ
Sbjct: 421 DTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQ 480
Query: 481 NTGISIHACKILATADLQPSKSSFPTFLGRPWKLYSRVVYMVSSIGDHIHPRGWLEWQGS 540
NTGISIHACKILAT DL+ SK SFPTFLGRPWKLYSRVVYMVSS+GDHIHPRGWLEWQGS
Sbjct: 481 NTGISIHACKILATTDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGS 540
Query: 541 FALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTLEATKFTVGQFIYGSSWLPSTGVSF 600
FALDTLYYGEYMNSGPGAA GQRV WPGYRVITST+EATKFTVGQFIYGSSWLPSTGVSF
Sbjct: 541 FALDTLYYGEYMNSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSF 592
Query: 601 SAGLSAN 606
SAGLSAN
Sbjct: 601 SAGLSAN 592
BLAST of Tan0005200 vs. ExPASy TrEMBL
Match:
A0A6J1K6C1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111491552 PE=3 SV=1)
HSP 1 Score: 1035.4 bits (2676), Expect = 9.4e-299
Identity = 530/607 (87.31%), Postives = 554/607 (91.27%), Query Frame = 0
Query: 1 MNNYNKLHSSVSGDTPTTSELSHDPNRGPSLPPPLSSTSRRRTRILFISLLSILLITASA 60
MNNYNKLHSS SGDTP T ++SH PN PP SS SRRRTRILF+SL+SILLITASA
Sbjct: 1 MNNYNKLHSSGSGDTPATFQVSHTPN---PPHPPSSSASRRRTRILFVSLISILLITASA 60
Query: 61 VSAAVLIRSHIGGATSSEAHFPHNKPSQAISRACSLTLYPSLCLSSLLSFPGSLSADVHD 120
VSAAVLI SH +H PHNKP+QAIS ACS TLYPSLC SSLLSFPGSLSADVH
Sbjct: 61 VSAAVLIHSH-------SSHIPHNKPTQAISHACSRTLYPSLCRSSLLSFPGSLSADVHH 120
Query: 121 LVHITLNLTLQHLNKALYSSSQIPISQFSNNPLAHSAYEDCMELLNDAVDAFSLSLFSPA 180
LVHI+LNLTLQHL KALY+SSQIP+ Q SNNPLAHSAY+DC+ELLNDAVDAFSLSLFS +
Sbjct: 121 LVHISLNLTLQHLTKALYASSQIPVFQISNNPLAHSAYQDCIELLNDAVDAFSLSLFSTS 180
Query: 181 ASNHDVVTWLSAALTYHDTCMDGFADVADPVKDQMAAKLSDLSEMISNSLAIFSGFGGGL 240
ASNHD++TWLSAALTYHDTC DGFADV DPVKDQMA++LSDLSEMISNSLAIFSG+G GL
Sbjct: 181 ASNHDIMTWLSAALTYHDTCSDGFADVDDPVKDQMASRLSDLSEMISNSLAIFSGYGEGL 240
Query: 241 PVQNRRRRRLMDSGSEWAAENG--VTEGEFPEWVSGKDRRLLATPISAIQADIVVAKDGS 300
PVQN RRRLM+S EWAAENG V EGEFP WVSGKDRRLLA ISAIQADIVVAKDGS
Sbjct: 241 PVQN--RRRLMESDREWAAENGVAVAEGEFPGWVSGKDRRLLAASISAIQADIVVAKDGS 300
Query: 301 GKFKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 360
GKFKTVAEAI AAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK
Sbjct: 301 GKFKTVAEAIEAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 360
Query: 361 SIFDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQ 420
S+FDKVTTFRTATFAGSGTGIILRDMT ENTAGPSKHQAVALR+SADHAVVY CNIIGYQ
Sbjct: 361 SVFDKVTTFRTATFAGSGTGIILRDMTIENTAGPSKHQAVALRVSADHAVVYRCNIIGYQ 420
Query: 421 DTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKDPNQ 480
DTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQ+RKDPNQ
Sbjct: 421 DTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQNRKDPNQ 480
Query: 481 NTGISIHACKILATADLQPSKSSFPTFLGRPWKLYSRVVYMVSSIGDHIHPRGWLEWQGS 540
NTGISIHACKILAT DL+ SK SF TFLGRPWKLYSRVVYMVSS+GDHIHPRGWLEWQGS
Sbjct: 481 NTGISIHACKILATTDLEASKGSFSTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGS 540
Query: 541 FALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTLEATKFTVGQFIYGSSWLPSTGVSF 600
FALDTLYYGEYMNSGPGAA GQRV WPGYRVITST+EATKFTVGQFIYGSSWLPSTGVSF
Sbjct: 541 FALDTLYYGEYMNSGPGAATGQRVNWPGYRVITSTIEATKFTVGQFIYGSSWLPSTGVSF 595
Query: 601 SAGLSAN 606
SAGLS N
Sbjct: 601 SAGLSGN 595
BLAST of Tan0005200 vs. ExPASy TrEMBL
Match:
A0A6J1E2V1 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430323 PE=3 SV=1)
HSP 1 Score: 1017.3 bits (2629), Expect = 2.6e-293
Identity = 519/605 (85.79%), Postives = 553/605 (91.40%), Query Frame = 0
Query: 2 NNYNKLHSSVSGDTPTTSELSHDPNRGPSLPPPLSSTSRRRTRILFISLLSILLITASAV 61
NNYN LHSS SGDTP +S + + P LPPP S+SRR RIL IS SILLITASAV
Sbjct: 3 NNYNMLHSSHSGDTPASSFRVSNDHSDP-LPPPSPSSSRR--RILLISFFSILLITASAV 62
Query: 62 SAAVLIRSHIGGATSSEAHFPHNKPSQAISRACSLTLYPSLCLSSLLSFPGSLSADVHDL 121
SA VL+ S I A S+E+HFPHNKP+QAISRACSLTLYPSLCLSSLLS PGSLSADVHDL
Sbjct: 63 SAVVLVHSRI--AASTESHFPHNKPTQAISRACSLTLYPSLCLSSLLSAPGSLSADVHDL 122
Query: 122 VHITLNLTLQHLNKALYSSSQIPISQFSNNPLAHSAYEDCMELLNDAVDAFSLSLFSPAA 181
VHI+LNLTLQHL KALYS+SQIPISQ SN+PLA SAYEDCMELLNDAV+AFSLSLFS A
Sbjct: 123 VHISLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTA 182
Query: 182 SNHDVVTWLSAALTYHDTCMDGFADVADPVKDQMAAKLSDLSEMISNSLAIFSGFGG-GL 241
+NHDV+TWLSAALTYHDTC DGF+DVADP+KDQ+ AKLSDLSEMISNSLAIFSGFGG L
Sbjct: 183 TNHDVMTWLSAALTYHDTCTDGFSDVADPIKDQITAKLSDLSEMISNSLAIFSGFGGDDL 242
Query: 242 PVQNRRRRRLMDSGSEWAAENGVTEGEFPEWVSGKDRRLLATPISAIQADIVVAKDGSGK 301
PV+NRRRRRLM+S +W AE +G+FP WVSGKDRRLLA PIS+IQADIVVAKDGSGK
Sbjct: 243 PVENRRRRRLMESDEQWLAEENNGDGDFPRWVSGKDRRLLAAPISSIQADIVVAKDGSGK 302
Query: 302 FKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSI 361
FKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKS+
Sbjct: 303 FKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV 362
Query: 362 FDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDT 421
+DKVTTFRTATFAGSGTG+ILRDMTFENTAGP+KHQAVALRLSADHAVV+ N+IGYQDT
Sbjct: 363 YDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHAVVFRSNVIGYQDT 422
Query: 422 LYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKDPNQNT 481
LYVHSNRQFFRECDIYGT+DFIFGNAVVVFQSCNIYARKPM QKNTITAQDRKDPNQNT
Sbjct: 423 LYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMSGQKNTITAQDRKDPNQNT 482
Query: 482 GISIHACKILATADLQPSKSSFPTFLGRPWKLYSRVVYMVSSIGDHIHPRGWLEWQGSFA 541
GISIHACKI+AT DLQ SK SFPT+LGRPWKLYSRVVYMVSS+GDHIHPRGWLEWQGSFA
Sbjct: 483 GISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGSFA 542
Query: 542 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTLEATKFTVGQFIYGSSWLPSTGVSFSA 601
LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITS +EATKFTVGQFIYGSSWLPSTGVSFSA
Sbjct: 543 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSAIEATKFTVGQFIYGSSWLPSTGVSFSA 602
Query: 602 GLSAN 606
GLS N
Sbjct: 603 GLSTN 602
BLAST of Tan0005200 vs. ExPASy TrEMBL
Match:
A0A6J1IA52 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111471502 PE=3 SV=1)
HSP 1 Score: 1014.2 bits (2621), Expect = 2.2e-292
Identity = 515/605 (85.12%), Postives = 548/605 (90.58%), Query Frame = 0
Query: 2 NNYNKLHSSVSGDTPTTSELSHDPNRGPSLPPPLSSTSRRRTRILFISLLSILLITASAV 61
NNYNKLHSS SGDTP TS + + P PPP SS+ R RIL IS SILLITAS V
Sbjct: 3 NNYNKLHSSRSGDTPVTSFRVSNDHSNPPPPPPPSSSHR---RILLISFFSILLITASTV 62
Query: 62 SAAVLIRSHIGGATSSEAHFPHNKPSQAISRACSLTLYPSLCLSSLLSFPGSLSADVHDL 121
SA VL+ S I TS+E+HFPHNKP+QAISRACSLTLYPSLC+SSLLS PGSLSADVHDL
Sbjct: 63 SAVVLVHSRI--TTSTESHFPHNKPTQAISRACSLTLYPSLCISSLLSAPGSLSADVHDL 122
Query: 122 VHITLNLTLQHLNKALYSSSQIPISQFSNNPLAHSAYEDCMELLNDAVDAFSLSLFSPAA 181
VHI+LNLTLQHL KALYS+SQIPISQ SN+PLA SAYEDCMELLNDAV+AFSLSLFS A
Sbjct: 123 VHISLNLTLQHLTKALYSTSQIPISQISNDPLAQSAYEDCMELLNDAVEAFSLSLFSTTA 182
Query: 182 SNHDVVTWLSAALTYHDTCMDGFADVADPVKDQMAAKLSDLSEMISNSLAIFSGFGGG-L 241
+NHDV+TWLSAALTYHDTC DGF+DVADPVKDQ+ AKLSDLSEMISNSLAIFSGFGGG L
Sbjct: 183 TNHDVMTWLSAALTYHDTCTDGFSDVADPVKDQITAKLSDLSEMISNSLAIFSGFGGGDL 242
Query: 242 PVQNRRRRRLMDSGSEWAAENGVTEGEFPEWVSGKDRRLLATPISAIQADIVVAKDGSGK 301
PV+NRRRRRLM+S +W AE +G+FP WVSGKDRRLLA PIS+IQADIVVAKDGSGK
Sbjct: 243 PVENRRRRRLMESDEQWLAEENNGDGDFPRWVSGKDRRLLAAPISSIQADIVVAKDGSGK 302
Query: 302 FKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSI 361
FKTVAEAIAAAPSSS RRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKS+
Sbjct: 303 FKTVAEAIAAAPSSSSRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSV 362
Query: 362 FDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDT 421
+DKVTTFRTATFAGSGTG+ILRDMTFENTAGP+KHQAVALRLSADH VV+ N+IGYQDT
Sbjct: 363 YDKVTTFRTATFAGSGTGLILRDMTFENTAGPNKHQAVALRLSADHTVVFRSNVIGYQDT 422
Query: 422 LYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKDPNQNT 481
LYVHSNRQFFRECDIYGT+DFIFGNA VVFQSCNIYARKPM QKNTITAQDRKDPNQNT
Sbjct: 423 LYVHSNRQFFRECDIYGTIDFIFGNAAVVFQSCNIYARKPMSGQKNTITAQDRKDPNQNT 482
Query: 482 GISIHACKILATADLQPSKSSFPTFLGRPWKLYSRVVYMVSSIGDHIHPRGWLEWQGSFA 541
GISIHACKI+AT DLQ SK SFPT+LGRPWKLYSRVVYMVSS+GDHIHPRGWLEWQGSFA
Sbjct: 483 GISIHACKIIATGDLQASKGSFPTYLGRPWKLYSRVVYMVSSLGDHIHPRGWLEWQGSFA 542
Query: 542 LDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTLEATKFTVGQFIYGSSWLPSTGVSFSA 601
LDTLYYGEYMNSGP AAVGQRVKWPGYRVITS +EATKFTVGQFIYGSSWLPSTGVSFSA
Sbjct: 543 LDTLYYGEYMNSGPSAAVGQRVKWPGYRVITSAIEATKFTVGQFIYGSSWLPSTGVSFSA 602
Query: 602 GLSAN 606
GLS N
Sbjct: 603 GLSTN 602
BLAST of Tan0005200 vs. ExPASy TrEMBL
Match:
A0A0A0KJG1 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G139430 PE=3 SV=1)
HSP 1 Score: 998.4 bits (2580), Expect = 1.3e-287
Identity = 510/607 (84.02%), Postives = 547/607 (90.12%), Query Frame = 0
Query: 2 NNYNKLHSSVSGDTPTTSELSHDPNRGPSLPPPLSSTSRRRTRILFISLLSILLITASAV 61
N+YNKLH+S S +T + N PPP SS+ RRR+RIL IS +S+LLI AS V
Sbjct: 5 NSYNKLHTSHSANTTPAASFRLSGND----PPPPSSSLRRRSRILIISFMSVLLIAASVV 64
Query: 62 SAAVLIRSHIGGATSSEAHFPHNKPSQAISRACSLTLYPSLCLSSLLSFPGSLSADVHDL 121
SAA+L+RSH+ TSS+ HFPHNKPSQAISRACS TLYPSLCLSSLLSFPG+ SADVHDL
Sbjct: 65 SAAILVRSHM---TSSQPHFPHNKPSQAISRACSHTLYPSLCLSSLLSFPGAQSADVHDL 124
Query: 122 VHITLNLTLQHLNKALYSSSQIPISQFSNNPLAHSAYEDCMELLNDAVDAFSLSLFSPAA 181
VHI+LNLTLQHL KALYS+SQIP+ Q S +PLAHSAYEDCMELLNDA+DAFSLSLFS A
Sbjct: 125 VHISLNLTLQHLTKALYSTSQIPVLQISKDPLAHSAYEDCMELLNDAIDAFSLSLFSKDA 184
Query: 182 SNHDVVTWLSAALTYHDTCMDGFADVAD-PVKDQMAAKLSDLSEMISNSLAIFSGFGGG- 241
SNHD++TWLSAALTYHDTC GF DVAD VKD++ AKLSDLSEMISNSLAIFSGFGGG
Sbjct: 185 SNHDIMTWLSAALTYHDTCTAGFQDVADLGVKDEVEAKLSDLSEMISNSLAIFSGFGGGD 244
Query: 242 LPVQNRRRRRLMDSG-SEWAAENGVTEGEFPEWVSGKDRRLLATPISAIQADIVVAKDGS 301
LPV+NR+RRRLM+S + WAAENG FP W+SGKDRRLLA P+S IQADIVVAKDGS
Sbjct: 245 LPVENRKRRRLMESSTTSWAAENGGDHEGFPAWLSGKDRRLLAAPLSTIQADIVVAKDGS 304
Query: 302 GKFKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 361
GKFKTVAEAI AAPSSSGRRI+IYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK
Sbjct: 305 GKFKTVAEAIEAAPSSSGRRIIIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGK 364
Query: 362 SIFDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQ 421
S++DKVTTFRTATFAGSGT IILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQ
Sbjct: 365 SVYDKVTTFRTATFAGSGTNIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQ 424
Query: 422 DTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKDPNQ 481
DTLYVHSNRQFFRECDIYGT+DFIFGNAVVVFQSCNIYARKPM QKNTITAQDRKDPNQ
Sbjct: 425 DTLYVHSNRQFFRECDIYGTIDFIFGNAVVVFQSCNIYARKPMAGQKNTITAQDRKDPNQ 484
Query: 482 NTGISIHACKILATADLQPSKSSFPTFLGRPWKLYSRVVYMVSSIGDHIHPRGWLEWQGS 541
NTGISIHACKI+AT DL+ SK SFPTFLGRPWKLYSRVVYMVSS+GDHIHPRGWLEWQGS
Sbjct: 485 NTGISIHACKIVATGDLEASKGSFPTFLGRPWKLYSRVVYMVSSMGDHIHPRGWLEWQGS 544
Query: 542 FALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTLEATKFTVGQFIYGSSWLPSTGVSF 601
FALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITST+EA+KFTVGQFIYGSSWLPSTGVSF
Sbjct: 545 FALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTVEASKFTVGQFIYGSSWLPSTGVSF 604
Query: 602 SAGLSAN 606
SAGLS N
Sbjct: 605 SAGLSTN 604
BLAST of Tan0005200 vs. TAIR 10
Match:
AT5G53370.1 (pectin methylesterase PCR fragment F )
HSP 1 Score: 696.4 bits (1796), Expect = 2.0e-200
Identity = 369/593 (62.23%), Postives = 438/593 (73.86%), Query Frame = 0
Query: 28 GPSLP------PPLSSTSRRRTRILFISLLSILLITASAVSAAVLIRSHIGGATSSEAHF 87
GPS P L ++ L + L++L++ IR+ G T +
Sbjct: 10 GPSNPNQKDPATSLPELQKKTKTKLILFTLAVLVVGVVCFGIFAGIRAVDSGKTEPKL-- 69
Query: 88 PHNKPSQAISRACSLTLYPSLCLSSLLSFPGSLSADVHDLVHITLNLTLQHLNKALYSSS 147
KP+QAISR CS +LYP+LC+ +LL FPGSL+AD ++L+HI+ N TLQ +KALY+SS
Sbjct: 70 -TRKPTQAISRTCSKSLYPNLCIDTLLDFPGSLTADENELIHISFNATLQKFSKALYTSS 129
Query: 148 QIPISQFSNNPLAHSAYEDCMELLNDAVDAFSLSLFSPAA-----SNHDVVTWLSAALTY 207
I +Q P SAY+ C+ELL+D+VDA + +L S S+ DV+TWLS+A+T
Sbjct: 130 TITYTQMP--PRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHSDVMTWLSSAMTN 189
Query: 208 HDTCMDGFADV---ADPVKDQMAAKLSDLSEMISNSLAIFSGFG---GGLPVQNRRRRRL 267
HDTC DGF ++ VKDQ+ + DLSEM+SN LAIF+G G+PV N R+
Sbjct: 190 HDTCTDGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVNNRKL-- 249
Query: 268 MDSGSEWAAENGVTEGEFPEWVSGKDRRLLATPISAIQADIVVAKDGSGKFKTVAEAIAA 327
G+E TE E P W+ +DR LL TP SAIQADI V+KDGSG FKT+AEAI
Sbjct: 250 --LGTE------ETE-ELPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKK 309
Query: 328 APSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIFDKVTTFRTA 387
AP S RR VIY+KAG+YEEENLKVGRKKTNLMF+GDGKG TVI+GGKSI D +TTF TA
Sbjct: 310 APEHSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTA 369
Query: 388 TFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFF 447
TFA +G G I+RDMTFEN AGP+KHQAVALR+ DHAVVY CNIIGYQD LYVHSNRQFF
Sbjct: 370 TFAATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFF 429
Query: 448 RECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKDPNQNTGISIHACKIL 507
REC+IYGTVDFIFGNA V+ QSCNIYARKPM QQK TITAQ+RKDPNQNTGISIHACK+L
Sbjct: 430 RECEIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLL 489
Query: 508 ATADLQPSKSSFPTFLGRPWKLYSRVVYMVSSIGDHIHPRGWLEWQGSFALDTLYYGEYM 567
AT DL+ SK S+PT+LGRPWKLYSRVVYM+S +GDHI PRGWLEW G FALD+LYYGEYM
Sbjct: 490 ATPDLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYM 549
Query: 568 NSGPGAAVGQRVKWPGYRVITSTLEATKFTVGQFIYGSSWLPSTGVSFSAGLS 604
N G G+ +GQRVKWPGY VITST+EA+KFTV QFI GSSWLPSTGVSF +GLS
Sbjct: 550 NKGLGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGLS 586
BLAST of Tan0005200 vs. TAIR 10
Match:
AT3G49220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )
HSP 1 Score: 682.2 bits (1759), Expect = 3.9e-196
Identity = 361/606 (59.57%), Postives = 444/606 (73.27%), Query Frame = 0
Query: 9 SSVSGDTPTTSELSHDPNRGPSLPPPLSSTSRRRTRILFISL-LSILLITASAVSAAVLI 68
S +G T++ + N+ + P ++ R + +++ S+ L+I LI A+A+ A V
Sbjct: 9 SGATGSVTTSTTTAPILNQVSTSEQPENNNRRSKKKLVVSSIVLAISLILAAAIFAGVRS 68
Query: 69 RSHIGGATSSEAHFPHNKPSQAISRACSLTLYPSLCLSSLLSFPGSLSA-DVHDLVHITL 128
R + + A KPSQAIS+AC LT +P LC+ SL+ FPGSL+A DL+H+T+
Sbjct: 69 RLKLNQSVPGLA----RKPSQAISKACELTRFPELCVDSLMDFPGSLAASSSKDLIHVTV 128
Query: 129 NLTLQHLNKALYSSSQIPISQFSNNPLAHSAYEDCMELLNDAVDAFSLSLFSPAASN--- 188
N+TL H + ALYSS+ +S P A SAY+ C+ELL+D+VDA S +L S +S+
Sbjct: 129 NMTLHHFSHALYSSAS--LSFVDMPPRARSAYDSCVELLDDSVDALSRALSSVVSSSAKP 188
Query: 189 HDVVTWLSAALTYHDTCMDGFADVAD-PVKDQMAAKLSDLSEMISNSLAIFSGFG----- 248
DV TWLSAALT HDTC +GF V D VKD M A L +LSE++SN LAIFS
Sbjct: 189 QDVTTWLSAALTNHDTCTEGFDGVDDGGVKDHMTAALQNLSELVSNCLAIFSASHDGDDF 248
Query: 249 GGLPVQNRRRRRLMDSGSEWAAENGVTEGEFPEWVSGKDRRLLATPISAIQADIVVAKDG 308
G+P+QNRR + + E +FP W+ K+R +L P+S IQADI+V+KDG
Sbjct: 249 AGVPIQNRRLLGVEE-----------REEKFPRWMRPKEREILEMPVSQIQADIIVSKDG 308
Query: 309 SGKFKTVAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGG 368
+G KT++EAI AP +S RRI+IY+KAG+YEE NLKVGRKK NLMFVGDGKG TVISGG
Sbjct: 309 NGTCKTISEAIKKAPQNSTRRIIIYVKAGRYEENNLKVGRKKINLMFVGDGKGKTVISGG 368
Query: 369 KSIFDKVTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGY 428
KSIFD +TTF TA+FA +G G I RD+TFEN AGP+KHQAVALR+ ADHAV+Y CNIIGY
Sbjct: 369 KSIFDNITTFHTASFAATGAGFIARDITFENWAGPAKHQAVALRIGADHAVIYRCNIIGY 428
Query: 429 QDTLYVHSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKDPN 488
QDTLYVHSNRQFFRECDIYGTVDFIFGNA VV Q+C+IYARKPM QKNTITAQ+RKDPN
Sbjct: 429 QDTLYVHSNRQFFRECDIYGTVDFIFGNAAVVLQNCSIYARKPMDFQKNTITAQNRKDPN 488
Query: 489 QNTGISIHACKILATADLQPSKSSFPTFLGRPWKLYSRVVYMVSSIGDHIHPRGWLEWQG 548
QNTGISIHA ++LA +DLQ + S T+LGRPWKL+SR VYM+S IG H+H RGWLEW
Sbjct: 489 QNTGISIHASRVLAASDLQATNGSTQTYLGRPWKLFSRTVYMMSYIGGHVHTRGWLEWNT 548
Query: 549 SFALDTLYYGEYMNSGPGAAVGQRVKWPGYRVITSTLEATKFTVGQFIYGSSWLPSTGVS 604
+FALDTLYYGEY+NSGPG+ +GQRV WPGYRVI ST EA +FTV +FIYGSSWLPSTGVS
Sbjct: 549 TFALDTLYYGEYLNSGPGSGLGQRVSWPGYRVINSTAEANRFTVAEFIYGSSWLPSTGVS 597
BLAST of Tan0005200 vs. TAIR 10
Match:
AT1G53830.1 (pectin methylesterase 2 )
HSP 1 Score: 443.4 bits (1139), Expect = 3.0e-124
Identity = 256/575 (44.52%), Postives = 340/575 (59.13%), Query Frame = 0
Query: 55 LITASAVSAAVLIRSHIGGATSSEAHFPHNK------PSQAISRA-CSLTLYPSLCLSSL 114
LI +SA A +L+ S +G A ++ + K S AI ++ CS TLYP LC S++
Sbjct: 20 LILSSAAIALLLLASIVGIAATTTNQNKNQKITTLSSTSHAILKSVCSSTLYPELCFSAV 79
Query: 115 LSFPGSLSADVHDLVHITLNLTLQHLNKALYSSSQIPISQFSNNPLAHSAYEDCMELLND 174
+ G +++ +LNLT + + ++ ++ + P +A DC+E +++
Sbjct: 80 AATGGKELTSQKEVIEASLNLTTKAVKHNYFAVKKLIAKRKGLTPREVTALHDCLETIDE 139
Query: 175 AVDAFSLSL---------FSPAASNHDVVTWLSAALTYHDTCMDGFA--DVADPVKDQMA 234
+D +++ S D+ T +S+A+T TC+DGF+ D V+ +
Sbjct: 140 TLDELHVAVEDLHQYPKQKSLRKHADDLKTLISSAITNQGTCLDGFSYDDADRKVRKALL 199
Query: 235 AKLSDLSEMISNSLAIFSGFGGGLPVQNRRRRRLMDSGSEWAAENG---------VTEGE 294
+ M SN+LA+ + + L D S + N +
Sbjct: 200 KGQVHVEHMCSNALAMIK----NMTETDIANFELRDKSSTFTNNNNRKLKEVTGDLDSDG 259
Query: 295 FPEWVSGKDRRLLATPISAIQADIVVAKDGSGKFKTVAEAIAAAPSSSGRRIVIYIKAGK 354
+P+W+S DRRLL S I+AD VA DGSG F TVA A+AAAP S +R VI+IKAG
Sbjct: 260 WPKWLSVGDRRLLQG--STIKADATVADDGSGDFTTVAAAVAAAPEKSNKRFVIHIKAGV 319
Query: 355 YEEENLKVGRKKTNLMFVGDGKGITVISGGKSIFDKVTTFRTATFAGSGTGIILRDMTFE 414
Y EN++V +KKTN+MF+GDG+G T+I+G +++ D TTF +AT A G + RD+TF+
Sbjct: 320 Y-RENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGSTTFHSATVAAVGERFLARDITFQ 379
Query: 415 NTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGNAV 474
NTAGPSKHQAVALR+ +D + Y C++ YQDTLYVHSNRQFF +C I GTVDFIFGNA
Sbjct: 380 NTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVHSNRQFFVKCHITGTVDFIFGNAA 439
Query: 475 VVFQSCNIYARKPMPQQKNTITAQDRKDPNQNTGISIHACKILATADLQPSKSSFPTFLG 534
V Q C+I AR+P QKN +TAQ R DPNQNTGI I C+I T+DL K +FPT+LG
Sbjct: 440 AVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVIQNCRIGGTSDLLAVKGTFPTYLG 499
Query: 535 RPWKLYSRVVYMVSSIGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRVKWPGY 594
RPWK YSR V M S I D I P GW EW GSFALDTL Y EY+N G GA RVKW GY
Sbjct: 500 RPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTLTYREYLNRGGGAGTANRVKWKGY 559
Query: 595 RVITSTLEATKFTVGQFIYGSSWLPSTGVSFSAGL 603
+VITS EA FT GQFI G WL STG FS L
Sbjct: 560 KVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587
BLAST of Tan0005200 vs. TAIR 10
Match:
AT3G14310.1 (pectin methylesterase 3 )
HSP 1 Score: 442.6 bits (1137), Expect = 5.2e-124
Identity = 255/599 (42.57%), Postives = 359/599 (59.93%), Query Frame = 0
Query: 29 PSLPPPLSSTSRRRTRILFISLLSILLITASAVSAAVLIRSHIGGATSSEAHFPHNKPSQ 88
PS+ S + ++ + L + ++ L+ +AV+ GA+ + + S
Sbjct: 3 PSMKEIFSKDNFKKNKKLVLLSAAVALLFVAAVAGI------SAGASKANEKRTLSPSSH 62
Query: 89 AISR-ACSLTLYPSLCLSSLLSFPGSLSADVHDLVHITLNLTLQHLNKALYSSSQIPISQ 148
A+ R +CS T YP LC+S++++ G D++ ++NLT+ + ++ ++ +
Sbjct: 63 AVLRSSCSSTRYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLIKKR 122
Query: 149 FSNNPLAHSAYEDCMELLNDAVDAF-----SLSLF--SPAASNH--DVVTWLSAALTYHD 208
P +A DC+E +++ +D L L+ H D+ T +S+A+T +
Sbjct: 123 KGLTPREKTALHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAITNQE 182
Query: 209 TCMDGFA--DVADPVKDQMAAKLSDLSEMISNSLAIFSGFGGGLPVQNRRRRRLMDSGSE 268
TC+DGF+ D V+ + + M SN+LA+ ++ ++ + +
Sbjct: 183 TCLDGFSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSNNRK 242
Query: 269 WAAENGVT--------EGE-----FPEWVSGKDRRLLATPISAIQADIVVAKDGSGKFKT 328
EN T GE +P W+S DRRLL S ++AD VA DGSG FKT
Sbjct: 243 LKEENQETTVAVDIAGAGELDSEGWPTWLSAGDRRLLQG--SGVKADATVAADGSGTFKT 302
Query: 329 VAEAIAAAPSSSGRRIVIYIKAGKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIFDK 388
VA A+AAAP +S +R VI+IKAG Y EN++V +KK N+MF+GDG+ T+I+G +++ D
Sbjct: 303 VAAAVAAAPENSNKRYVIHIKAGVY-RENVEVAKKKKNIMFMGDGRTRTIITGSRNVVDG 362
Query: 389 VTTFRTATFAGSGTGIILRDMTFENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYV 448
TTF +AT A G + RD+TF+NTAGPSKHQAVALR+ +D + Y+C+++ YQDTLYV
Sbjct: 363 STTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLYV 422
Query: 449 HSNRQFFRECDIYGTVDFIFGNAVVVFQSCNIYARKPMPQQKNTITAQDRKDPNQNTGIS 508
HSNRQFF +C I GTVDFIFGNA VV Q C+I+AR+P QKN +TAQ R DPNQNTGI
Sbjct: 423 HSNRQFFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGIV 482
Query: 509 IHACKILATADLQPSKSSFPTFLGRPWKLYSRVVYMVSSIGDHIHPRGWLEWQGSFALDT 568
I C+I AT+DLQ K SFPT+LGRPWK YS+ V M S+I D I P GW EW G+FAL+T
Sbjct: 483 IQKCRIGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALNT 542
Query: 569 LYYGEYMNSGPGAAVGQRVKWPGYRVITSTLEATKFTVGQFIYGSSWLPSTGVSFSAGL 603
L Y EY N+G GA RVKW G++VIT+ EA K+T GQFI G WL STG FS GL
Sbjct: 543 LTYREYSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592
BLAST of Tan0005200 vs. TAIR 10
Match:
AT3G43270.1 (Plant invertase/pectin methylesterase inhibitor superfamily )
HSP 1 Score: 429.9 bits (1104), Expect = 3.5e-120
Identity = 226/457 (49.45%), Postives = 300/457 (65.65%), Query Frame = 0
Query: 156 SAYEDCMELLNDAVDAFSLSLFSPAASN----------HDVVTWLSAALTYHDTCMDGFA 215
+A DC++LL+ A + S + + + N D+ TW+SAAL+ DTC+DGF
Sbjct: 79 NAIVDCVDLLDSAAEELSWIISASQSPNGKDNSTGDVGSDLRTWISAALSNQDTCLDGFE 138
Query: 216 DVADPVKDQMAAKLSDLSEMISNSLAIFSGFGGGLPVQNRRRRRLMDSGSEWAAENGVTE 275
+K +A LS + + N L + ++ + + + + + A +G +
Sbjct: 139 GTNGIIKKIVAGGLSKVGTTVRNLLTMVHS------PPSKPKPKPIKAQTMTKAHSGFS- 198
Query: 276 GEFPEWVSGKDRRLLATPISAIQADIVVAKDGSGKFKTVAEAIAAAPSSSGRRIVIYIKA 335
+FP WV DR+LL T + AD VVA DG+G F T+++A+ AAP S +R VI++K
Sbjct: 199 -KFPSWVKPGDRKLLQTDNITV-ADAVVAADGTGNFTTISDAVLAAPDYSTKRYVIHVKR 258
Query: 336 GKYEEENLKVGRKKTNLMFVGDGKGITVISGGKSIFDKVTTFRTATFAGSGTGIILRDMT 395
G Y EN+++ +KK N+M VGDG TVI+G +S D TTFR+ATFA SG G I RD+T
Sbjct: 259 GVY-VENVEIKKKKWNIMMVGDGIDATVITGNRSFIDGWTTFRSATFAVSGRGFIARDIT 318
Query: 396 FENTAGPSKHQAVALRLSADHAVVYHCNIIGYQDTLYVHSNRQFFRECDIYGTVDFIFGN 455
F+NTAGP KHQAVA+R D V Y C + GYQDTLY HS RQFFREC I GTVDFIFG+
Sbjct: 319 FQNTAGPEKHQAVAIRSDTDLGVFYRCAMRGYQDTLYAHSMRQFFRECIITGTVDFIFGD 378
Query: 456 AVVVFQSCNIYARKPMPQQKNTITAQDRKDPNQNTGISIHACKILATADLQPSKSSFPTF 515
A VFQSC I A++ +P QKN+ITAQ RKDPN+ TG +I I A DL + ++ T+
Sbjct: 379 ATAVFQSCQIKAKQGLPNQKNSITAQGRKDPNEPTGFTIQFSNIAADTDLLLNLNTTATY 438
Query: 516 LGRPWKLYSRVVYMVSSIGDHIHPRGWLEWQGSFALDTLYYGEYMNSGPGAAVGQRVKWP 575
LGRPWKLYSR V+M + + D I+P GWLEW G+FALDTLYYGEYMNSGPGA++ +RVKWP
Sbjct: 439 LGRPWKLYSRTVFMQNYMSDAINPVGWLEWNGNFALDTLYYGEYMNSGPGASLDRRVKWP 498
Query: 576 GYRVITSTLEATKFTVGQFIYGSSWLPSTGVSFSAGL 603
GY V+ ++ EA FTV Q I G+ WLPSTG++F AGL
Sbjct: 499 GYHVLNTSAEANNFTVSQLIQGNLWLPSTGITFIAGL 525
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| Q9FK05 | 2.8e-199 | 62.23 | Probable pectinesterase/pectinesterase inhibitor 61 OS=Arabidopsis thaliana OX=3... | [more] |
| Q9M3B0 | 5.4e-195 | 59.57 | Probable pectinesterase/pectinesterase inhibitor 34 OS=Arabidopsis thaliana OX=3... | [more] |
| O04886 | 2.9e-124 | 44.27 | Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1 | [more] |
| P83948 | 2.9e-124 | 43.94 | Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1 | [more] |
| Q43143 | 8.6e-124 | 43.15 | Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PM... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| XP_022957644.1 | 8.4e-302 | 87.97 | probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita moschata] | [more] |
| XP_023533011.1 | 7.1e-301 | 87.81 | probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita pepo subsp. pepo] | [more] |
| KAG6605784.1 | 2.3e-299 | 87.31 | putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosp... | [more] |
| XP_022996280.1 | 1.9e-298 | 87.31 | probable pectinesterase/pectinesterase inhibitor 61 [Cucurbita maxima] | [more] |
| KAG6579321.1 | 1.9e-293 | 85.79 | putative pectinesterase/pectinesterase inhibitor 61, partial [Cucurbita argyrosp... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A6J1H2K1 | 4.1e-302 | 87.97 | Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111459120 PE=3 SV=1 | [more] |
| A0A6J1K6C1 | 9.4e-299 | 87.31 | Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111491552 PE=3 SV=1 | [more] |
| A0A6J1E2V1 | 2.6e-293 | 85.79 | Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111430323 PE=3 SV=1 | [more] |
| A0A6J1IA52 | 2.2e-292 | 85.12 | Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111471502 PE=3 SV=1 | [more] |
| A0A0A0KJG1 | 1.3e-287 | 84.02 | Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_5G139430 PE=3 SV=1 | [more] |
