Tan0004946 (gene) Snake gourd v1

Overview
NameTan0004946
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionEnolase-phosphatase E1
LocationLG01: 7898461 .. 7904145 (-)
RNA-Seq ExpressionTan0004946
SyntenyTan0004946
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GAGATATTGAAAAATGAATGTGAAAAAAATTTGAAAAATTATAGCAAAAATGGTTTTTTTTTTTTAAAAAAAAACTCATACTTAGAGAACTCTTATCCAGACTCTATCCTTCATCTCTGCTCTCTGTTGAATCACTCAAATGGCAATTGCTTCAGCTCTGGCCGTCGATGGAGTCAATTTGGCTTCGACTTCTCAAGCTTATTTCGAAAACAAGGCTCTCTCAACGGACCTCTGCCACCAAGTCCACGGCCTCCGATGGGTCTCCCGCGCCCGCGGTAGCATTGCCATCAAGGTTCACGACCACTCCAGTCCCAAGAGACAACAGCCAATCGCTATGTCACCTTCTGGTGGGCATTTCTCCATCTCTCAATTCTTTCGTTCCTTGTTCAATTTCTTACACTTTCTGGGCATTTTGTTACTCGCAATTGTATTGCTTCCAATTGAGCCTAATTGGATTTGTTCGCCTCTTGGGTAAGTGATTATGGAGGGTTCCCTCAGTTTTCCCTGTAATCTTTTTAGGTGTAGAATTTTTGTTATTGAGGTAATTCCGTCTGCCTCAATTCTGATTTCGGTTGTGTTTAAAAACACTTAGATTAGTGTGTTTTACTCAAAAGTACGACCTCAAAATCATCTATCAAACCCACCCTTAAGGTGTGTTTAAGAGTGCTTTTAAATTAAGGGTTAGGTTTAATAATTTTTTTTTTTAATTTATGAGAAACTTATAATCATTTTATTGATGGTATGAAATGTACAAAAGGGGCCCAGTGATAAAGGGGTGAAGGGACCCAATTACAAAAGATTTTCCTAATTACTAATAAGGGAAGTAAGAACATAGTCACCAAAAAAAAGTAGAGAATTTACACCAAGAGAAAGTAAGGTAGAGAAGGTGCTCTAGAACAATGTGGGGATCTCTTTCGATGTCTTTGAAAAGCCTGTTATTTCGTTCAAGCCAAATGGATAATAGAAGAGCTTTAACAATGTTTTCTCAAATGCATGCCTTTCCTTGTGTGTATGGATGACCCAACAGGAGAGCCTAGGAGTGTAATCATAGTGTGTGGGAAAACAAAATGTAGCTTTGCAGCCTTAAGGATTTTTGACCAAATACTGGACGTGTATGAGCAAGTGGAGAAGATATGGCCCATACATTCACATGCCTTTTTGCATAGAACACACCAAGTAGGAGCAGATACTAACCATGGAGCCTTTTCATTTTGAAGTGTATCAGCAGTGTTGATCCCTTCATGGCTGACTTCCCAAAGGAAAAACCAAATTTTTTTGGGGATGTGTCCTTTCCAGATGGTTCGGTAAAGTTGTTTGTGTGAGAGGTTGTTGCAGGATGATAACCTATGAGTGAGGGATTTAGCTGTGAAAGATCCCTTATTGTCTAACCTCCACTTCCAAGAGTCATCAACCAAGGGCCTAATAGTATTTGGGAGGGTTAGGTTAGGTTTTGAAATGCTTCTCAATCAAGCACCTAAGCTAAAAGTAAATGCTTTCCTATTAAACAATTTGAGGTGCTTTTGACTTTTTTGGAAACCACTCTTGTGTTGATTTTGGACGGGGAGAGGTTCTGGCCACTCCCAAATATAGCCTAACTCTTCTCTGCTTCTTAGCTTTTAGGTTATTAGTCTGTTAAACAATGCCAGGCATCGTTATAATTCAAACTAGTGCTTGTTAGGAGGATATCCAATTCCCCCTTCTTTTTGCAGAAGAAGAACTTGGTCTTCTTTTGAACCAAAAATGCTTTGCAAGGGTTCAAGCCATCAAGAACTAGACTAAAGAGTGCATTTTTTCATGGTGCAAGGTCACCTGCTTTCTGTATTTTCGTTATCTTTTTTTTTTGTTTGGCAAGAGGGTATTATATAAAATTCAACTCCTTACATAAGTGTAAAAAGTATAAAGAGCTTGTTCCTTCTTTTGTTTCTTCTTTTACCCTAAAAAGGGGATTGCTTGGAATTCATGGATTTTGATGAAAACTCTTTTGCTTTTGTTGGATTCTACATAGGTGTTAAGAAGGATAGAATGGTACTGGAGGATATCTTTGTGTTATCTCATGGTGGATCTATCTTATATTCACCCCCTTCAAAGCCATTTTCACATAAGCTTCCAAACCGCTGGGGACCTCTTTTTATGAAGGTACAGTTCCCAGTACACTGTGAAATACTCATTTGGACGAAGACTTGCAAAGACATCATGAGCTAAAACCATGCTGACAAATAATCAACTCGAAATGGCCCAGCATCTATATTTATGATCATATAAACAATGTCTCCTATACTTCGAGACATTAACTTCACTGATATCTGCATGATAAACCAATCTTTTCTCATTCTTATTCGGTCCAGATCCATCCATAACAACCAAATTTTTTTCCTGTTTTCCAGGCATATGAGATGCGCAAAAGATCAAGACATTGGTTTTGTTAAATACTTCTAACAAGAAGTTCCTTTGAACTTTGTAAATGATGAAACCTTTTATGATTTTGAGTCTGTCTTGATCTTCCATTGGTTTCAGTTTAATCAATAACTTAATTTTACAGGCTGGTTGTTATCACTATCTCTTTGATGCTGCTATAAGCTTCCACAACTAGGCATTGATTAGTCTTCTCCGACTCAGGGTCCGATTGCGAGCATCAAAGGAATTTCAAGAGATAACGTTGGCATCAACATATCTGTGAAGGCTGGGGCTCAAGGTTTAGATAGTAAAACTGAACCATTGCCAGTGAGTTTAATTGCATTCCGCTTAATTCGGAAAGCTTAAAATTTCAGCCTTTAATTTTTCTTCCTTTTCTTTTCACTTTGAGGTATGCCATTTTTGTGTCTTGGTTAGGAGGTTTTTGTTTATAGTTTGAGTTCAATCGGTCTTATTTTGGATGCAGCGTTGCATAGTTCTTGATATTGAAGGGGCTACTACTCCAATATCTTTTGTTACTGACGTACTCTTTCCGTATGCTCGTGATAATGTTGGAAAGCATTTGATTTTAACATACGAAACTGCAGAAACTCAAGAGGACATTAAGTTGTTGCGTGAACAAGTAAGATATTTCGGTTGAACAGATTTTGATTATGTTGCAGTTTTGAGAATATTATGTTCCATTTATGGTATTTACTTGGAGATTGTTAGTCTTGGTTAAACTGAAAAATAGCTTGTGCGTCTGAGTTCATCTGTGAAATACAGAAACCTCCTCCTAGCAGCAAACTCAATTTGGTACATGAGAGGCTATTTTGGAGTTGTTTTCTGAATTCTGTTTTTTAGTATAAAGATTCAGAGAAGGGTATGTTTGTATAACTAAATTCTAGAAGTTCTAAATTTGCTGATACATTGCTACGTCATCGATGGCGAGCATCCTACATAGATTTGTTTCTTGTAATCAAACCAATTGATGCTTCTAGTTTGACAACTGGCCAAGCAGTAAATACTGTTACATTTTTGGCACAGGAAGGGCCTTCTTAGTAATTATCAATAAAAATGTTTTCAGGTTGAAGACGACCTGGAAAAAGGTGTTGCTGGAGCTGTGCCTATTCCTCCTGATAATGCTGGGAAAGAGGAAGTCATTGCAGCATTGGTTGGTAATGTTGAAGCAATGATAAAGGCTGACCGCAAGATAACTGCCTTGAAACAATTGCAAGTATGCAAGTAGAATAATTCTCTATTTATCAACTGCAATTCGTTTAGCAGATTTTCTAGTGAGCTGACTTCTACCTATGTAGGGTCACGTATGGAGAACAGGATTTTCAGGCAATGAATTGGAGGGAGTAGTTTTTGATGATGTGCCAGAGGCTCTCGAGAGATGGCATGCTTTGGGCATTAAGGTCATTCCACCCCACCCCCCACCCCCGAATTTTTGCTAAAACCAGTCGATGCAGATAACAAGAAAAATATCACACGTATACTAACAAGTTGCTAGTCGAGACTAACCGGGGAAAAGTCCTCCCAAGGCTCTAACTCCTATATCGATATAGACCGAACTATCTCAGGATGTTTAGAACCCAACTCACATCTTGCTTTGATGGAAGAATGACCCAACCTTTTTCAACGTACTATGACCCCAAGTGGCAAAGAAATGATGTATTAGTCTCTGACATTTTGAATCTCGTTATTAATAGAATCAAAATGATCTTGGATTGAAGCTATGGTGGTTACCTACCTAAGATTTAACATCCTACAAGTTACTTTGGCAATCAAATATAGTTAAGTCAAGCAGTTGTCCCGTGAGAATAGTTGGAGTGCGTGCAAACTAGCTCAGACACTCACAGATATAAAAAAAATCAAAATGGTCTTTATCGATCTCTCCCAATTCAACCATCCAAGTGTTCTCTAAATTGCACCGATACTTGGAGTAGACTAAACAAAAGCTTACTTAAGTCACTTTGGAGCAAAAATTTTGCATGCGTACACAATGGAATCTATTTAGAGTTTTAGGGAAGACTAAACTCAGTTATTAGGCGAAATTGACGGTTTTATACTTGGGTTAGTAGATGCCAGGCTTCTCTAGTAATAGATGTTTTGTGATTGATTTCCCTTCATTCTCATTTGGAAGCCTGGTGATATTTGAACAAAAGTTCTCCAACAGACATTACGAACCAGTAATAACTTCTATCATTTCTGGGATGAACAGGTCTACATATACTCAAGTGGCAGCAGGTTGGCACAAAGGCTTATATTTGGGAAGACTAATTATGGTGATCTAAGAAAATACTTATCAGGATTCTTTGACACTGCTGTGGGGTAACAAATTGCTGTTTTTTTTCCATGTTTTAATCATACTCATATTCAATACCACAAACATGAGTGTGGTACTGCTCTTGTATCTCAGGAACAAAAGAGAAATAAACAGTTATGTTGAGATTTCAGAGTCTGTTGGAGTTGATACACCATCAGAGATATTGTTCATCACAGATGTCTATCAGGAAGCTATAGCAGCAAAAGCAGCAGGTAATGAGTCTCTACTTTTAGCTATTGTTTGTTGTTAGTTTGTGGAAGAAAAAGGAGTAAAATCCATTCATGCTATAACCATGCATTTGGCTGAAAGTAGAATTGCTAAGTTAATCGTAATGCAGAATTACTAATTTCATACTTCTTCCATGCAATCGACAACGTTTCTCAGGGCTGCAGGTGGCAATCTCAATCCGACCAGGAAATAGTCCACTGCCTGATAATCACGGGTTCCAGACAATCAATTCCTTCTCGGAAATCTGAAATGAGCAACTACATTTTGTAATTACATTCATAAAAAAACCTGAAAATGATCAATAGCATCATAGATTAACAATACTTTACCCTGAGAGACTGTTCCATTCAGCAAGCTTGCCCAATTTTTTCCTCTCTGCCATTGTTGTTTAAAGTTTGAACTGAAGAAATATCTTTTTTGTTTGTACCAGAATAGTTGTACTTAAATCTTGTCCTGATCAAGCCTATGTAATAAACTAAGCTCAAAATGCCAGTTTTTCACTTTTGAAGTATTGTAAGTAAATCAAATAACAGATACTCTGTGCCCATTTTAGCATCAAATTTCAGTTTGGTTCATTAGGTCGTCCTTTGAAGAATTATTTTACTACGTTCAAATCACTAAGAAGCCAAAAGTTTAAGCCAATGAGTTAGAAAAAATTTAATCATTTAGATTTATTCTTACCAATCTCTTTTACTTGCGAGTTTGAAAATTAGTAAAAAAAACCAAAATTAAAG

mRNA sequence

GAGATATTGAAAAATGAATGTGAAAAAAATTTGAAAAATTATAGCAAAAATGGTTTTTTTTTTTTAAAAAAAAACTCATACTTAGAGAACTCTTATCCAGACTCTATCCTTCATCTCTGCTCTCTGTTGAATCACTCAAATGGCAATTGCTTCAGCTCTGGCCGTCGATGGAGTCAATTTGGCTTCGACTTCTCAAGCTTATTTCGAAAACAAGGCTCTCTCAACGGACCTCTGCCACCAAGTCCACGGCCTCCGATGGGTCTCCCGCGCCCGCGGTAGCATTGCCATCAAGGTTCACGACCACTCCAGTCCCAAGAGACAACAGCCAATCGCTATGTCACCTTCTGGTGTTAAGAAGGATAGAATGGTACTGGAGGATATCTTTGTGTTATCTCATGGTGGATCTATCTTATATTCACCCCCTTCAAAGCCATTTTCACATAAGCTTCCAAACCGCTGGGGACCTCTTTTTATGAAGGGTCCGATTGCGAGCATCAAAGGAATTTCAAGAGATAACGTTGGCATCAACATATCTGTGAAGGCTGGGGCTCAAGGTTTAGATAGTAAAACTGAACCATTGCCACGTTGCATAGTTCTTGATATTGAAGGGGCTACTACTCCAATATCTTTTGTTACTGACGTACTCTTTCCGTATGCTCGTGATAATGTTGGAAAGCATTTGATTTTAACATACGAAACTGCAGAAACTCAAGAGGACATTAAGTTGTTGCGTGAACAAGTTGAAGACGACCTGGAAAAAGGTGTTGCTGGAGCTGTGCCTATTCCTCCTGATAATGCTGGGAAAGAGGAAGTCATTGCAGCATTGGTTGGTAATGTTGAAGCAATGATAAAGGCTGACCGCAAGATAACTGCCTTGAAACAATTGCAAGGTCACGTATGGAGAACAGGATTTTCAGGCAATGAATTGGAGGGAGTAGTTTTTGATGATGTGCCAGAGGCTCTCGAGAGATGGCATGCTTTGGGCATTAAGGTCTACATATACTCAAGTGGCAGCAGGTTGGCACAAAGGCTTATATTTGGGAAGACTAATTATGGTGATCTAAGAAAATACTTATCAGGATTCTTTGACACTGCTGTGGGGAACAAAAGAGAAATAAACAGTTATGTTGAGATTTCAGAGTCTGTTGGAGTTGATACACCATCAGAGATATTGTTCATCACAGATGTCTATCAGGAAGCTATAGCAGCAAAAGCAGCAGGGCTGCAGGTGGCAATCTCAATCCGACCAGGAAATAGTCCACTGCCTGATAATCACGGGTTCCAGACAATCAATTCCTTCTCGGAAATCTGAAATGAGCAACTACATTTTGTAATTACATTCATAAAAAAACCTGAAAATGATCAATAGCATCATAGATTAACAATACTTTACCCTGAGAGACTGTTCCATTCAGCAAGCTTGCCCAATTTTTTCCTCTCTGCCATTGTTGTTTAAAGTTTGAACTGAAGAAATATCTTTTTTGTTTGTACCAGAATAGTTGTACTTAAATCTTGTCCTGATCAAGCCTATGTAATAAACTAAGCTCAAAATGCCAGTTTTTCACTTTTGAAGTATTGTAAGTAAATCAAATAACAGATACTCTGTGCCCATTTTAGCATCAAATTTCAGTTTGGTTCATTAGGTCGTCCTTTGAAGAATTATTTTACTACGTTCAAATCACTAAGAAGCCAAAAGTTTAAGCCAATGAGTTAGAAAAAATTTAATCATTTAGATTTATTCTTACCAATCTCTTTTACTTGCGAGTTTGAAAATTAGTAAAAAAAACCAAAATTAAAG

Coding sequence (CDS)

ATGGCAATTGCTTCAGCTCTGGCCGTCGATGGAGTCAATTTGGCTTCGACTTCTCAAGCTTATTTCGAAAACAAGGCTCTCTCAACGGACCTCTGCCACCAAGTCCACGGCCTCCGATGGGTCTCCCGCGCCCGCGGTAGCATTGCCATCAAGGTTCACGACCACTCCAGTCCCAAGAGACAACAGCCAATCGCTATGTCACCTTCTGGTGTTAAGAAGGATAGAATGGTACTGGAGGATATCTTTGTGTTATCTCATGGTGGATCTATCTTATATTCACCCCCTTCAAAGCCATTTTCACATAAGCTTCCAAACCGCTGGGGACCTCTTTTTATGAAGGGTCCGATTGCGAGCATCAAAGGAATTTCAAGAGATAACGTTGGCATCAACATATCTGTGAAGGCTGGGGCTCAAGGTTTAGATAGTAAAACTGAACCATTGCCACGTTGCATAGTTCTTGATATTGAAGGGGCTACTACTCCAATATCTTTTGTTACTGACGTACTCTTTCCGTATGCTCGTGATAATGTTGGAAAGCATTTGATTTTAACATACGAAACTGCAGAAACTCAAGAGGACATTAAGTTGTTGCGTGAACAAGTTGAAGACGACCTGGAAAAAGGTGTTGCTGGAGCTGTGCCTATTCCTCCTGATAATGCTGGGAAAGAGGAAGTCATTGCAGCATTGGTTGGTAATGTTGAAGCAATGATAAAGGCTGACCGCAAGATAACTGCCTTGAAACAATTGCAAGGTCACGTATGGAGAACAGGATTTTCAGGCAATGAATTGGAGGGAGTAGTTTTTGATGATGTGCCAGAGGCTCTCGAGAGATGGCATGCTTTGGGCATTAAGGTCTACATATACTCAAGTGGCAGCAGGTTGGCACAAAGGCTTATATTTGGGAAGACTAATTATGGTGATCTAAGAAAATACTTATCAGGATTCTTTGACACTGCTGTGGGGAACAAAAGAGAAATAAACAGTTATGTTGAGATTTCAGAGTCTGTTGGAGTTGATACACCATCAGAGATATTGTTCATCACAGATGTCTATCAGGAAGCTATAGCAGCAAAAGCAGCAGGGCTGCAGGTGGCAATCTCAATCCGACCAGGAAATAGTCCACTGCCTGATAATCACGGGTTCCAGACAATCAATTCCTTCTCGGAAATCTGA

Protein sequence

MAIASALAVDGVNLASTSQAYFENKALSTDLCHQVHGLRWVSRARGSIAIKVHDHSSPKRQQPIAMSPSGVKKDRMVLEDIFVLSHGGSILYSPPSKPFSHKLPNRWGPLFMKGPIASIKGISRDNVGINISVKAGAQGLDSKTEPLPRCIVLDIEGATTPISFVTDVLFPYARDNVGKHLILTYETAETQEDIKLLREQVEDDLEKGVAGAVPIPPDNAGKEEVIAALVGNVEAMIKADRKITALKQLQGHVWRTGFSGNELEGVVFDDVPEALERWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREINSYVEISESVGVDTPSEILFITDVYQEAIAAKAAGLQVAISIRPGNSPLPDNHGFQTINSFSEI
Homology
BLAST of Tan0004946 vs. ExPASy Swiss-Prot
Match: E0CSI1 (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 OS=Vitis vinifera OX=29760 GN=VIT_19s0014g02480 PE=3 SV=2)

HSP 1 Score: 488.0 bits (1255), Expect = 9.7e-137
Identity = 280/516 (54.26%), Postives = 321/516 (62.21%), Query Frame = 0

Query: 5   SALAVDGVNLASTSQAYFEN------KALSTDLCHQVHGLRWVSRARGSIAIKVHDHSSP 64
           +A A++G+ +A+TSQAY E+      + L +DLC   +GL WVS   GSI IKVHD S P
Sbjct: 2   AAAALNGLKMAATSQAYLESMPVNDTRKLLSDLCRHFYGLGWVSGTGGSITIKVHDESIP 61

Query: 65  KRQQPIAMSPSGVKKDRMVLEDIFVLSHGGSILYSPPSKPFSHKLP--NRWGPLFMKG-- 124
           K QQ I MSPSGV+K+RMV ED++VLS  G  L SP  KP+ +K P  +   PLFMK   
Sbjct: 62  KPQQLIVMSPSGVQKERMVPEDMYVLSPSGCFLSSPSPKPYPNKPPKCSDCAPLFMKAYL 121

Query: 125 ----------------------PIAS---------IKGISRD------------------ 184
                                 P++          IKGI                     
Sbjct: 122 MRNAGAVIHSHGMESCIVTMIDPLSKEFRITHMEMIKGIQGHGYYDELVVPIIENTAHER 181

Query: 185 ----------------------NVGI---------------------------------- 244
                                 N GI                                  
Sbjct: 182 ELTDALAEAIEAYPKTTAVLVRNHGIYIWGDSWIHAKTQAECYHYLFDAAIKLYQLGLDW 241

Query: 245 ---------------NISVKAGAQGLDSKTEPLPRCIVLDIEGATTPISFVTDVLFPYAR 304
                          N+SVKAG    + +TEP  RCIVLDIEG TTPISFVTDVLFP+AR
Sbjct: 242 STPDHGPIKKYGGKTNMSVKAGTVNSNHETEPSRRCIVLDIEGTTTPISFVTDVLFPFAR 301

Query: 305 DNVGKHLILTYETAETQEDIKLLREQVEDDLEKGVAGAVPIPPDNAGKEEVIAALVGNVE 364
           +NV +HL  TYET ETQ+DIKLLR QV+ DLE+G+ GAVPIPPD+AGKEEVIAALV NVE
Sbjct: 302 NNVSRHLAATYETDETQDDIKLLRTQVQSDLEQGIVGAVPIPPDDAGKEEVIAALVANVE 361

Query: 365 AMIKADRKITALKQLQGHVWRTGFSGNELEGVVFDDVPEALERWHALGIKVYIYSSGSRL 391
           AMIKADRKITALKQLQGH+WRTGF  NELEGVVF+DVPEAL++WHA GIKVYIYSSGSRL
Sbjct: 362 AMIKADRKITALKQLQGHIWRTGFQNNELEGVVFEDVPEALQKWHASGIKVYIYSSGSRL 421

BLAST of Tan0004946 vs. ExPASy Swiss-Prot
Match: B9N1F9 (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS=Populus trichocarpa OX=3694 GN=POPTR_0001s40980g PE=3 SV=2)

HSP 1 Score: 484.2 bits (1245), Expect = 1.4e-135
Identity = 279/516 (54.07%), Postives = 322/516 (62.40%), Query Frame = 0

Query: 2   AIASALAVDGVNLAS---TSQAYFENKA------LSTDLCHQVHGLRWVSRARGSIAIKV 61
           A   A+AV+G  +A+    SQAY E+KA      L  DLC Q + L WVS   GSI IK 
Sbjct: 3   AAPPAVAVNGGGMAAAKVASQAYLESKAVKDTRVLIADLCKQFYTLGWVSGTGGSITIKA 62

Query: 62  HDHSSPKRQQPIAMSPSGVKKDRMVLEDIFVLSHGGSILYSPPSKPFSHKLP--NRWGPL 121
           HD S PKRQQ I MSPSGV+K+RM  ED++VL+  GSIL SP  KP+ +K P  +   PL
Sbjct: 63  HDDSIPKRQQLILMSPSGVQKERMEPEDMYVLATNGSILSSPSPKPYPYKPPKCSDCAPL 122

Query: 122 FMK------------------------GPIAS---------IKGISRD------------ 181
           F+K                         P++          IKGI               
Sbjct: 123 FLKAYDMRNAGAVIHSHGMESCLVTMINPLSKEFRITHMEMIKGIQGHGYYDELVVPIIE 182

Query: 182 ----------------------------NVGI---------------------------- 241
                                       N GI                            
Sbjct: 183 NTAYENELTDSLAKAIEAYPKTTAVLVRNHGIYIWGDSWISAKTQAECYHYLFDAAIKLH 242

Query: 242 ---------------NISVKAGAQGLDSKTEPLPRCIVLDIEGATTPISFVTDVLFPYAR 301
                          N+ VKAG    +++ EPLPRCIVLDIEG TTPI+FV DVLFPYAR
Sbjct: 243 QIGLDWSTPNHGPIQNVKVKAGMNNSNNRIEPLPRCIVLDIEGTTTPITFVADVLFPYAR 302

Query: 302 DNVGKHLILTYETAETQEDIKLLREQVEDDLEKGVAGAVPIPPDNAGKEEVIAALVGNVE 361
           DNVG+HL  TY+TAET++DI LLR QVEDDL +GV GA+PIP D+AGKEEVIAALV NVE
Sbjct: 303 DNVGRHLSATYDTAETKDDINLLRTQVEDDLAQGVDGAIPIPTDDAGKEEVIAALVANVE 362

Query: 362 AMIKADRKITALKQLQGHVWRTGFSGNELEGVVFDDVPEALERWHALGIKVYIYSSGSRL 391
           AMIKADRKITALKQLQGH+WRTG+  NELEGVV+DDVPEALE+WHALGIKVYIYSSGSRL
Sbjct: 363 AMIKADRKITALKQLQGHIWRTGYENNELEGVVYDDVPEALEKWHALGIKVYIYSSGSRL 422

BLAST of Tan0004946 vs. ExPASy Swiss-Prot
Match: B9SQI7 (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS=Ricinus communis OX=3988 GN=RCOM_0591060 PE=3 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 2.4e-135
Identity = 281/525 (53.52%), Postives = 316/525 (60.19%), Query Frame = 0

Query: 2   AIASALAVDGVNLASTSQAYFENKA------LSTDLCHQVHGLRWVSRARGSIAIKVHDH 61
           A A A+AV+G  +   SQAY E+KA      L +DLC Q + L WVS   GSI IKVHD 
Sbjct: 4   AAAPAVAVNG-GVKVASQAYLESKAVKETRVLISDLCRQFYNLGWVSGTGGSITIKVHDD 63

Query: 62  SSPKRQQPIAMSPSGVKKDRMVLEDIFVLSHGGSILYSPPSKPFSHKLP--NRWGPLFMK 121
           S PK  Q I MSPSGV+K+RM  ED++VL+  GSIL SP  KP+ HK P  +  GPLF+K
Sbjct: 64  SIPKPNQLILMSPSGVQKERMEPEDMYVLAANGSILSSPSPKPYPHKPPKCSDCGPLFLK 123

Query: 122 ------------------------------------------------------------ 181
                                                                       
Sbjct: 124 AYEMCNAGAVIHSHGMESCLVTMINPLSKEFKITHMEMIKGIKGHGYYDELVVPIIENTA 183

Query: 182 ------------------------------------------------------------ 241
                                                                       
Sbjct: 184 YENELTDSLAKAIEEYPKTTAVLVRNHGIYIWGDSWISAKTQAECYHYLFDAAIKLHQLG 243

Query: 242 --------GPIASIKGISRDNVGINISVKAGAQGLDSKTEPLPRCIVLDIEGATTPISFV 301
                   G I ++K +   N  IN SVKAG +  +   + LP CIVLDIEG TTPI+FV
Sbjct: 244 LDWSTPNHGLIQNVKALIGSNRDINTSVKAGLKDSNHGMQSLPGCIVLDIEGTTTPITFV 303

Query: 302 TDVLFPYARDNVGKHLILTYETAETQEDIKLLREQVEDDLEKGVAGAVPIPPDNAGKEEV 361
            DVLFPYARDNVG+HL  TYETAETQ+DIKLLR QVEDDL +GV  AV IPPD+AGKEEV
Sbjct: 304 ADVLFPYARDNVGRHLYATYETAETQDDIKLLRTQVEDDLARGVNEAVSIPPDDAGKEEV 363

Query: 362 IAALVGNVEAMIKADRKITALKQLQGHVWRTGFSGNELEGVVFDDVPEALERWHALGIKV 391
           IAALV NVEAMIKADRKITALKQLQGH+WRTGF  NELEGVV+DDVPEALE+WHALGIKV
Sbjct: 364 IAALVANVEAMIKADRKITALKQLQGHIWRTGFQNNELEGVVYDDVPEALEKWHALGIKV 423

BLAST of Tan0004946 vs. ExPASy Swiss-Prot
Match: E0CTF3 (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 2 OS=Vitis vinifera OX=29760 GN=VIT_12s0028g03470 PE=2 SV=2)

HSP 1 Score: 429.9 bits (1104), Expect = 3.1e-119
Identity = 240/498 (48.19%), Postives = 286/498 (57.43%), Query Frame = 0

Query: 23  ENKALSTDLCHQVHGLRWVSRARGSIAIKVHDHSSPKRQQPIAMSPSGVKKDRMVLEDIF 82
           E + L+++LC  ++ L W S   GSI IKVHD S PK +Q + +SPSGV+K+RMV ED++
Sbjct: 34  EARVLASELCRHMYTLGWFSGTGGSITIKVHDDSIPKPRQLVVISPSGVQKERMVPEDMY 93

Query: 83  VLSHGGSILYSPPSKPFSHKLP--NRWGPLFMK--------------------------- 142
           VLS  G IL +PP KP+ HK P      PLFMK                           
Sbjct: 94  VLSSDGFILSTPPLKPYPHKPPKCTDCTPLFMKVYEMRDAGAVIHSHGMESCIVTMILPF 153

Query: 143 ------------------------------------------------------------ 202
                                                                       
Sbjct: 154 SKEFRITHMEMIKGIKGHGYHDELVVPIIENTAHEAELVESLTEAITAYPKTTAVLVRNH 213

Query: 203 -----------------------------------------GPIASIKGISRDNVGINIS 262
                                                    GPI SI GI   N  ++  
Sbjct: 214 GVYVWGDSWISAKTQAECYHYLFDAAIKLHQLGLDWSTPTHGPIRSINGIWGCNGTMSRG 273

Query: 263 VKAGAQGLDSKTEPLPRCIVLDIEGATTPISFVTDVLFPYARDNVGKHLILTYETAETQE 322
           +K G   LD   EP  RCI+LDIEG TTPISFVTDVLFPYA  NVGKHL  T+++ ETQ+
Sbjct: 274 LKVGGLSLDDMIEPSQRCILLDIEGTTTPISFVTDVLFPYAHANVGKHLAATFDSEETQD 333

Query: 323 DIKLLREQVEDDLEKGVAGAVPIPPDNAGKEEVIAALVGNVEAMIKADRKITALKQLQGH 382
           DI LLR Q++ DLE GV GAVPIPPD  GKE VIA+ V NVEAMI+ADR ITALKQLQGH
Sbjct: 334 DINLLRSQIQHDLEHGVVGAVPIPPDYVGKELVIASFVANVEAMIRADRNITALKQLQGH 393

Query: 383 VWRTGFSGNELEGVVFDDVPEALERWHALGIKVYIYSSGSRLAQRLIFGKTNYGDLRKYL 391
           +W+TGF  NEL GVVFDDVPEALERWHA GIKVYIYSSGSR AQ+LIF  +NYGDLRKY 
Sbjct: 394 IWKTGFQSNELVGVVFDDVPEALERWHASGIKVYIYSSGSREAQQLIFSNSNYGDLRKYF 453

BLAST of Tan0004946 vs. ExPASy Swiss-Prot
Match: Q9FN41 (Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 OS=Arabidopsis thaliana OX=3702 GN=At5g53850 PE=1 SV=1)

HSP 1 Score: 424.9 bits (1091), Expect = 1.0e-117
Identity = 246/507 (48.52%), Postives = 302/507 (59.57%), Query Frame = 0

Query: 7   LAVDGVNLASTSQAYFENK------ALSTDLCHQVHGLRWVSRARGSIAIKVHDHSSPKR 66
           +AV    +    QAY E K      +L T+LC   +   WVS   GSI +KVHD S PK 
Sbjct: 1   MAVAAAAMIGLPQAYLEGKEVKETSSLVTELCRHFYTQGWVSGTGGSITMKVHDASIPKP 60

Query: 67  QQPIAMSPSGVKKDRMVLEDIFVLSHGGSILYSPPSKPFSHKLP--NRWGPLFMKG---- 126
           +Q I MSPSGV+K+RM  ED+++LS  GSI+ +P  KP+ +K P      PLFMK     
Sbjct: 61  EQLIVMSPSGVQKERMQPEDMYILSANGSIISTPSPKPYPNKPPKCTDCAPLFMKAYEMR 120

Query: 127 -----------------------------PIASIKGISRD-------------------- 186
                                         +  IKGI                       
Sbjct: 121 NAGAVIHSHGMESCLVTMLNPQAKEFRITHMEMIKGIQGHGYYDELVVPIIENTAYENEL 180

Query: 187 --------------------NVGINI---------------------SVKAGAQGLDSKT 246
                               N G+ I                     ++K    GLD+ T
Sbjct: 181 TDSLTKAIEAYPKATAVLVRNHGVYIWGDSWIHAKTQAECYHYLFDAAIKLHQLGLDAAT 240

Query: 247 ---------------------EPLPRCIVLDIEGATTPISFVTDVLFPYARDNVGKHLIL 306
                                E   R IVLDIEG TTPI+FVTDVLFPYAR+NVGKHL L
Sbjct: 241 PDHGPIRRTIHSQIKDSQYEREWPRRWIVLDIEGTTTPITFVTDVLFPYARENVGKHLNL 300

Query: 307 TYETAETQEDIKLLREQVEDDLEKGVAGAVPIPPDNAGKEEVIAALVGNVEAMIKADRKI 366
           TY TAETQEDIKLLR QVE+DL +GV GAVPIP  + GKE+VIAA+V NVEAMI+ADRKI
Sbjct: 301 TYHTAETQEDIKLLRAQVEEDLREGVTGAVPIPHADEGKEKVIAAMVSNVEAMIRADRKI 360

Query: 367 TALKQLQGHVWRTGFSGNELEGVVFDDVPEALERWHALGIKVYIYSSGSRLAQRLIFGKT 391
           TALK+LQGH+WRTGF  +EL+ +VF+DV +ALE+WH+ GIKVYIYSSGSRLAQ+L+FG T
Sbjct: 361 TALKELQGHIWRTGFECDELKAIVFEDVADALEKWHSSGIKVYIYSSGSRLAQKLLFGNT 420

BLAST of Tan0004946 vs. NCBI nr
Match: XP_038899594.1 (probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 [Benincasa hispida])

HSP 1 Score: 628.6 bits (1620), Expect = 3.5e-176
Identity = 329/398 (82.66%), Postives = 352/398 (88.44%), Query Frame = 0

Query: 1   MAIASALAVDGVNLASTSQAYFEN------KALSTDLCHQVHGLRWVSRARGSIAIKVHD 60
           MA+ASALAV+G+ LA+TSQAY E+      KAL ++L  Q  GLRWVS   GSIAI VHD
Sbjct: 1   MAMASALAVNGLKLATTSQAYLESKAANDTKALLSELSRQFDGLRWVSGTGGSIAITVHD 60

Query: 61  HSSPKRQQPIAMSPSGVKKDRMVLEDIFVLSHGGSILYSPPSKPFSHKLPNRW--GPLFM 120
           +S PKR++ IAMSPSGVKK+RM+ ED   LS+ GSILYSPP KPFS++LPN +  GPLFM
Sbjct: 61  YSIPKRRRLIAMSPSGVKKERMLREDTCKLSYAGSILYSPPPKPFSYELPNCFDCGPLFM 120

Query: 121 KGPIASIKGISRDNVGINISVKAGAQGLDSKTEPLPRCIVLDIEGATTPISFVTDVLFPY 180
           KG I SIKGIS      NISVKAG QGLD+K E LPRCIVLDIEG TTPISFVTDVLFPY
Sbjct: 121 KGRIGSIKGISS-----NISVKAGVQGLDNKNESLPRCIVLDIEGTTTPISFVTDVLFPY 180

Query: 181 ARDNVGKHLILTYETAETQEDIKLLREQVEDDLEKGVAGAVPIPPDNAGKEEVIAALVGN 240
           ARDNVGKHLILTYETAET++DIKLLR QVE+DLEKGVAGAVPIPPDNAGKEEVIAALV N
Sbjct: 181 ARDNVGKHLILTYETAETRDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVAN 240

Query: 241 VEAMIKADRKITALKQLQGHVWRTGFSGNELEGVVFDDVPEALERWHALGIKVYIYSSGS 300
           VEAMIKADRKITALKQLQGH+WRTGFSGNELEGVVFDDVPEALERWHA GIKVYIYSSGS
Sbjct: 241 VEAMIKADRKITALKQLQGHIWRTGFSGNELEGVVFDDVPEALERWHASGIKVYIYSSGS 300

Query: 301 RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREINSYVEISESVGVDTPSEILFITDVYQ 360
           RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREI+SYVEISESVGVDTPSEILFITDV Q
Sbjct: 301 RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREISSYVEISESVGVDTPSEILFITDVCQ 360

Query: 361 EAIAAKAAGLQVAISIRPGNSPLPDNHGFQTINSFSEI 391
           EA+AAKAAGLQVAISIRPGN PLPDNHGFQTI SFSEI
Sbjct: 361 EAVAAKAAGLQVAISIRPGNGPLPDNHGFQTITSFSEI 393

BLAST of Tan0004946 vs. NCBI nr
Match: XP_023551888.1 (probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 [Cucurbita pepo subsp. pepo] >XP_023551890.1 probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 616.3 bits (1588), Expect = 1.8e-172
Identity = 320/398 (80.40%), Postives = 344/398 (86.43%), Query Frame = 0

Query: 1   MAIASALAVDGVNLASTSQAYFE------NKALSTDLCHQVHGLRWVSRARGSIAIKVHD 60
           MA+AS LAV+GV LA+ S AY +       KAL  +L  Q +GLR VSR  GSIAIKVHD
Sbjct: 1   MAMASPLAVNGVKLATASLAYVDTKAANNTKALMAELSRQFYGLRLVSRPAGSIAIKVHD 60

Query: 61  HSSPKRQQPIAMSPSGVKKDRMVLEDIFVLSHGGSILYSPPSKPFSHKLPN--RWGPLFM 120
           HS PK Q  +A+S SGVKK+RMV ED  +LS+G SILYSPP KPFSHKLPN    GPLFM
Sbjct: 61  HSIPKGQHTVALSHSGVKKERMVAEDTSMLSYGVSILYSPPPKPFSHKLPNCLDGGPLFM 120

Query: 121 KGPIASIKGISRDNVGINISVKAGAQGLDSKTEPLPRCIVLDIEGATTPISFVTDVLFPY 180
           K P   IKGIS       ISVKAG QGLD++ +PLPRCIVLDIEG TTPISFVTDVLFPY
Sbjct: 121 KDPFGRIKGISS-----KISVKAGTQGLDNENKPLPRCIVLDIEGTTTPISFVTDVLFPY 180

Query: 181 ARDNVGKHLILTYETAETQEDIKLLREQVEDDLEKGVAGAVPIPPDNAGKEEVIAALVGN 240
           ARD+VGKHL+LTYETAETQ+DIKLLR QVE+DLEKGVAGAVPIPPDNAGKEEVIAALV N
Sbjct: 181 ARDSVGKHLLLTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVAN 240

Query: 241 VEAMIKADRKITALKQLQGHVWRTGFSGNELEGVVFDDVPEALERWHALGIKVYIYSSGS 300
           VEAMIKADRKITALKQLQGH+WRTGFSGNELEG+VFDDVPEALERWHALGIKVYIYSSGS
Sbjct: 241 VEAMIKADRKITALKQLQGHIWRTGFSGNELEGIVFDDVPEALERWHALGIKVYIYSSGS 300

Query: 301 RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREINSYVEISESVGVDTPSEILFITDVYQ 360
           RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREI SYVEISES+GVDTPSEILFITDVYQ
Sbjct: 301 RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQ 360

Query: 361 EAIAAKAAGLQVAISIRPGNSPLPDNHGFQTINSFSEI 391
           EA+AAKAAGLQVAISIRPGN PLPD+HGFQT  SFS+I
Sbjct: 361 EAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDI 393

BLAST of Tan0004946 vs. NCBI nr
Match: XP_022923008.1 (probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X3 [Cucurbita moschata])

HSP 1 Score: 614.8 bits (1584), Expect = 5.3e-172
Identity = 319/398 (80.15%), Postives = 344/398 (86.43%), Query Frame = 0

Query: 1   MAIASALAVDGVNLASTSQAYFE------NKALSTDLCHQVHGLRWVSRARGSIAIKVHD 60
           MA+AS LAV+GV LA+ S AY +       KAL  +L  Q +GLR VS   G I+IKVHD
Sbjct: 1   MAMASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHD 60

Query: 61  HSSPKRQQPIAMSPSGVKKDRMVLEDIFVLSHGGSILYSPPSKPFSHKLPN--RWGPLFM 120
           HS PK Q  +A+S SGVKK+RMV ED  +LS+GGSILYSPP KPFSHKLP     GPLFM
Sbjct: 61  HSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFM 120

Query: 121 KGPIASIKGISRDNVGINISVKAGAQGLDSKTEPLPRCIVLDIEGATTPISFVTDVLFPY 180
           K P  SIKG+S       ISVKAGAQGLD++ +PLPRCIVLDIEG TTPISFVTDVLFPY
Sbjct: 121 KDPFGSIKGMSS-----KISVKAGAQGLDNENKPLPRCIVLDIEGTTTPISFVTDVLFPY 180

Query: 181 ARDNVGKHLILTYETAETQEDIKLLREQVEDDLEKGVAGAVPIPPDNAGKEEVIAALVGN 240
           ARDNVGKHLILTYETAETQ+DIKLLR QVE+DLEKGVAGAVPIPPDNAGKEEVIAALV N
Sbjct: 181 ARDNVGKHLILTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVAN 240

Query: 241 VEAMIKADRKITALKQLQGHVWRTGFSGNELEGVVFDDVPEALERWHALGIKVYIYSSGS 300
           VEAMIKADRKITALKQLQGH+WRTGF+GNELEG+VFDDVPEALERWHALGIKVYIYSSGS
Sbjct: 241 VEAMIKADRKITALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIKVYIYSSGS 300

Query: 301 RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREINSYVEISESVGVDTPSEILFITDVYQ 360
           RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREI SYVEISES+GVDTPSEILFITDVYQ
Sbjct: 301 RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQ 360

Query: 361 EAIAAKAAGLQVAISIRPGNSPLPDNHGFQTINSFSEI 391
           EA+AAKAAGLQVAISIRPGN PLPD+HGFQT  SFS+I
Sbjct: 361 EAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDI 393

BLAST of Tan0004946 vs. NCBI nr
Match: XP_022923007.1 (probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X2 [Cucurbita moschata])

HSP 1 Score: 612.8 bits (1579), Expect = 2.0e-171
Identity = 318/398 (79.90%), Postives = 343/398 (86.18%), Query Frame = 0

Query: 1   MAIASALAVDGVNLASTSQAYFE------NKALSTDLCHQVHGLRWVSRARGSIAIKVHD 60
           +A AS LAV+GV LA+ S AY +       KAL  +L  Q +GLR VS   G I+IKVHD
Sbjct: 14  LATASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHD 73

Query: 61  HSSPKRQQPIAMSPSGVKKDRMVLEDIFVLSHGGSILYSPPSKPFSHKLPN--RWGPLFM 120
           HS PK Q  +A+S SGVKK+RMV ED  +LS+GGSILYSPP KPFSHKLP     GPLFM
Sbjct: 74  HSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFM 133

Query: 121 KGPIASIKGISRDNVGINISVKAGAQGLDSKTEPLPRCIVLDIEGATTPISFVTDVLFPY 180
           K P  SIKG+S       ISVKAGAQGLD++ +PLPRCIVLDIEG TTPISFVTDVLFPY
Sbjct: 134 KDPFGSIKGMSS-----KISVKAGAQGLDNENKPLPRCIVLDIEGTTTPISFVTDVLFPY 193

Query: 181 ARDNVGKHLILTYETAETQEDIKLLREQVEDDLEKGVAGAVPIPPDNAGKEEVIAALVGN 240
           ARDNVGKHLILTYETAETQ+DIKLLR QVE+DLEKGVAGAVPIPPDNAGKEEVIAALV N
Sbjct: 194 ARDNVGKHLILTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVAN 253

Query: 241 VEAMIKADRKITALKQLQGHVWRTGFSGNELEGVVFDDVPEALERWHALGIKVYIYSSGS 300
           VEAMIKADRKITALKQLQGH+WRTGF+GNELEG+VFDDVPEALERWHALGIKVYIYSSGS
Sbjct: 254 VEAMIKADRKITALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIKVYIYSSGS 313

Query: 301 RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREINSYVEISESVGVDTPSEILFITDVYQ 360
           RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREI SYVEISES+GVDTPSEILFITDVYQ
Sbjct: 314 RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQ 373

Query: 361 EAIAAKAAGLQVAISIRPGNSPLPDNHGFQTINSFSEI 391
           EA+AAKAAGLQVAISIRPGN PLPD+HGFQT  SFS+I
Sbjct: 374 EAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDI 406

BLAST of Tan0004946 vs. NCBI nr
Match: XP_022923004.1 (probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X1 [Cucurbita moschata] >XP_022923005.1 probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X1 [Cucurbita moschata] >XP_022923006.1 probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 612.8 bits (1579), Expect = 2.0e-171
Identity = 318/398 (79.90%), Postives = 343/398 (86.18%), Query Frame = 0

Query: 1   MAIASALAVDGVNLASTSQAYFE------NKALSTDLCHQVHGLRWVSRARGSIAIKVHD 60
           +A AS LAV+GV LA+ S AY +       KAL  +L  Q +GLR VS   G I+IKVHD
Sbjct: 27  LATASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHD 86

Query: 61  HSSPKRQQPIAMSPSGVKKDRMVLEDIFVLSHGGSILYSPPSKPFSHKLPN--RWGPLFM 120
           HS PK Q  +A+S SGVKK+RMV ED  +LS+GGSILYSPP KPFSHKLP     GPLFM
Sbjct: 87  HSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFM 146

Query: 121 KGPIASIKGISRDNVGINISVKAGAQGLDSKTEPLPRCIVLDIEGATTPISFVTDVLFPY 180
           K P  SIKG+S       ISVKAGAQGLD++ +PLPRCIVLDIEG TTPISFVTDVLFPY
Sbjct: 147 KDPFGSIKGMSS-----KISVKAGAQGLDNENKPLPRCIVLDIEGTTTPISFVTDVLFPY 206

Query: 181 ARDNVGKHLILTYETAETQEDIKLLREQVEDDLEKGVAGAVPIPPDNAGKEEVIAALVGN 240
           ARDNVGKHLILTYETAETQ+DIKLLR QVE+DLEKGVAGAVPIPPDNAGKEEVIAALV N
Sbjct: 207 ARDNVGKHLILTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVAN 266

Query: 241 VEAMIKADRKITALKQLQGHVWRTGFSGNELEGVVFDDVPEALERWHALGIKVYIYSSGS 300
           VEAMIKADRKITALKQLQGH+WRTGF+GNELEG+VFDDVPEALERWHALGIKVYIYSSGS
Sbjct: 267 VEAMIKADRKITALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIKVYIYSSGS 326

Query: 301 RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREINSYVEISESVGVDTPSEILFITDVYQ 360
           RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREI SYVEISES+GVDTPSEILFITDVYQ
Sbjct: 327 RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQ 386

Query: 361 EAIAAKAAGLQVAISIRPGNSPLPDNHGFQTINSFSEI 391
           EA+AAKAAGLQVAISIRPGN PLPD+HGFQT  SFS+I
Sbjct: 387 EAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDI 419

BLAST of Tan0004946 vs. ExPASy TrEMBL
Match: A0A6J1E8E3 (probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111430820 PE=4 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 2.5e-172
Identity = 319/398 (80.15%), Postives = 344/398 (86.43%), Query Frame = 0

Query: 1   MAIASALAVDGVNLASTSQAYFE------NKALSTDLCHQVHGLRWVSRARGSIAIKVHD 60
           MA+AS LAV+GV LA+ S AY +       KAL  +L  Q +GLR VS   G I+IKVHD
Sbjct: 1   MAMASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHD 60

Query: 61  HSSPKRQQPIAMSPSGVKKDRMVLEDIFVLSHGGSILYSPPSKPFSHKLPN--RWGPLFM 120
           HS PK Q  +A+S SGVKK+RMV ED  +LS+GGSILYSPP KPFSHKLP     GPLFM
Sbjct: 61  HSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFM 120

Query: 121 KGPIASIKGISRDNVGINISVKAGAQGLDSKTEPLPRCIVLDIEGATTPISFVTDVLFPY 180
           K P  SIKG+S       ISVKAGAQGLD++ +PLPRCIVLDIEG TTPISFVTDVLFPY
Sbjct: 121 KDPFGSIKGMSS-----KISVKAGAQGLDNENKPLPRCIVLDIEGTTTPISFVTDVLFPY 180

Query: 181 ARDNVGKHLILTYETAETQEDIKLLREQVEDDLEKGVAGAVPIPPDNAGKEEVIAALVGN 240
           ARDNVGKHLILTYETAETQ+DIKLLR QVE+DLEKGVAGAVPIPPDNAGKEEVIAALV N
Sbjct: 181 ARDNVGKHLILTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVAN 240

Query: 241 VEAMIKADRKITALKQLQGHVWRTGFSGNELEGVVFDDVPEALERWHALGIKVYIYSSGS 300
           VEAMIKADRKITALKQLQGH+WRTGF+GNELEG+VFDDVPEALERWHALGIKVYIYSSGS
Sbjct: 241 VEAMIKADRKITALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIKVYIYSSGS 300

Query: 301 RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREINSYVEISESVGVDTPSEILFITDVYQ 360
           RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREI SYVEISES+GVDTPSEILFITDVYQ
Sbjct: 301 RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQ 360

Query: 361 EAIAAKAAGLQVAISIRPGNSPLPDNHGFQTINSFSEI 391
           EA+AAKAAGLQVAISIRPGN PLPD+HGFQT  SFS+I
Sbjct: 361 EAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDI 393

BLAST of Tan0004946 vs. ExPASy TrEMBL
Match: A0A6J1EAE7 (probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430820 PE=4 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 9.7e-172
Identity = 318/398 (79.90%), Postives = 343/398 (86.18%), Query Frame = 0

Query: 1   MAIASALAVDGVNLASTSQAYFE------NKALSTDLCHQVHGLRWVSRARGSIAIKVHD 60
           +A AS LAV+GV LA+ S AY +       KAL  +L  Q +GLR VS   G I+IKVHD
Sbjct: 27  LATASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHD 86

Query: 61  HSSPKRQQPIAMSPSGVKKDRMVLEDIFVLSHGGSILYSPPSKPFSHKLPN--RWGPLFM 120
           HS PK Q  +A+S SGVKK+RMV ED  +LS+GGSILYSPP KPFSHKLP     GPLFM
Sbjct: 87  HSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFM 146

Query: 121 KGPIASIKGISRDNVGINISVKAGAQGLDSKTEPLPRCIVLDIEGATTPISFVTDVLFPY 180
           K P  SIKG+S       ISVKAGAQGLD++ +PLPRCIVLDIEG TTPISFVTDVLFPY
Sbjct: 147 KDPFGSIKGMSS-----KISVKAGAQGLDNENKPLPRCIVLDIEGTTTPISFVTDVLFPY 206

Query: 181 ARDNVGKHLILTYETAETQEDIKLLREQVEDDLEKGVAGAVPIPPDNAGKEEVIAALVGN 240
           ARDNVGKHLILTYETAETQ+DIKLLR QVE+DLEKGVAGAVPIPPDNAGKEEVIAALV N
Sbjct: 207 ARDNVGKHLILTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVAN 266

Query: 241 VEAMIKADRKITALKQLQGHVWRTGFSGNELEGVVFDDVPEALERWHALGIKVYIYSSGS 300
           VEAMIKADRKITALKQLQGH+WRTGF+GNELEG+VFDDVPEALERWHALGIKVYIYSSGS
Sbjct: 267 VEAMIKADRKITALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIKVYIYSSGS 326

Query: 301 RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREINSYVEISESVGVDTPSEILFITDVYQ 360
           RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREI SYVEISES+GVDTPSEILFITDVYQ
Sbjct: 327 RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQ 386

Query: 361 EAIAAKAAGLQVAISIRPGNSPLPDNHGFQTINSFSEI 391
           EA+AAKAAGLQVAISIRPGN PLPD+HGFQT  SFS+I
Sbjct: 387 EAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDI 419

BLAST of Tan0004946 vs. ExPASy TrEMBL
Match: A0A6J1E4Z2 (probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430820 PE=4 SV=1)

HSP 1 Score: 612.8 bits (1579), Expect = 9.7e-172
Identity = 318/398 (79.90%), Postives = 343/398 (86.18%), Query Frame = 0

Query: 1   MAIASALAVDGVNLASTSQAYFE------NKALSTDLCHQVHGLRWVSRARGSIAIKVHD 60
           +A AS LAV+GV LA+ S AY +       KAL  +L  Q +GLR VS   G I+IKVHD
Sbjct: 14  LATASPLAVNGVKLATASLAYVDTKAANHTKALMAELSRQFYGLRLVSGPAGCISIKVHD 73

Query: 61  HSSPKRQQPIAMSPSGVKKDRMVLEDIFVLSHGGSILYSPPSKPFSHKLPN--RWGPLFM 120
           HS PK Q  +A+S SGVKK+RMV ED  +LS+GGSILYSPP KPFSHKLP     GPLFM
Sbjct: 74  HSIPKGQHTVALSHSGVKKERMVAEDTCMLSYGGSILYSPPPKPFSHKLPYCLDGGPLFM 133

Query: 121 KGPIASIKGISRDNVGINISVKAGAQGLDSKTEPLPRCIVLDIEGATTPISFVTDVLFPY 180
           K P  SIKG+S       ISVKAGAQGLD++ +PLPRCIVLDIEG TTPISFVTDVLFPY
Sbjct: 134 KDPFGSIKGMSS-----KISVKAGAQGLDNENKPLPRCIVLDIEGTTTPISFVTDVLFPY 193

Query: 181 ARDNVGKHLILTYETAETQEDIKLLREQVEDDLEKGVAGAVPIPPDNAGKEEVIAALVGN 240
           ARDNVGKHLILTYETAETQ+DIKLLR QVE+DLEKGVAGAVPIPPDNAGKEEVIAALV N
Sbjct: 194 ARDNVGKHLILTYETAETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAALVAN 253

Query: 241 VEAMIKADRKITALKQLQGHVWRTGFSGNELEGVVFDDVPEALERWHALGIKVYIYSSGS 300
           VEAMIKADRKITALKQLQGH+WRTGF+GNELEG+VFDDVPEALERWHALGIKVYIYSSGS
Sbjct: 254 VEAMIKADRKITALKQLQGHIWRTGFAGNELEGIVFDDVPEALERWHALGIKVYIYSSGS 313

Query: 301 RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREINSYVEISESVGVDTPSEILFITDVYQ 360
           RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREI SYVEISES+GVDTPSEILFITDVYQ
Sbjct: 314 RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQ 373

Query: 361 EAIAAKAAGLQVAISIRPGNSPLPDNHGFQTINSFSEI 391
           EA+AAKAAGLQVAISIRPGN PLPD+HGFQT  SFS+I
Sbjct: 374 EAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDI 406

BLAST of Tan0004946 vs. ExPASy TrEMBL
Match: A0A6J1J246 (probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 OS=Cucurbita maxima OX=3661 GN=LOC111482722 PE=4 SV=1)

HSP 1 Score: 609.0 bits (1569), Expect = 1.4e-170
Identity = 316/398 (79.40%), Postives = 343/398 (86.18%), Query Frame = 0

Query: 1   MAIASALAVDGVNLASTSQAYFE------NKALSTDLCHQVHGLRWVSRARGSIAIKVHD 60
           MA+AS LAV+GV LA+TS AY +       KAL  +L  Q +GLR VS   GSIAIKVHD
Sbjct: 1   MAMASPLAVNGVKLATTSLAYVDTKAANNTKALMAELSRQFYGLRLVSSPAGSIAIKVHD 60

Query: 61  HSSPKRQQPIAMSPSGVKKDRMVLEDIFVLSHGGSILYSPPSKPFSHKLPN--RWGPLFM 120
           HS PK Q  I++S SGVKK+RM+ ED  +LS+GGSILYSPP KPFSHKLPN     PLFM
Sbjct: 61  HSIPKGQHTISLSHSGVKKERMLAEDTCMLSYGGSILYSPPPKPFSHKLPNCLDGRPLFM 120

Query: 121 KGPIASIKGISRDNVGINISVKAGAQGLDSKTEPLPRCIVLDIEGATTPISFVTDVLFPY 180
           K P  SIKGIS       ISVKAGAQGL+++ +PLPRCIVLDIEG TTPISFVTD+LFPY
Sbjct: 121 KDPFGSIKGISS-----KISVKAGAQGLNNENKPLPRCIVLDIEGTTTPISFVTDILFPY 180

Query: 181 ARDNVGKHLILTYETAETQEDIKLLREQVEDDLEKGVAGAVPIPPDNAGKEEVIAALVGN 240
           ARDNVGKHLILTYETAETQ+DI LLR QVE+DL+KGVA AVP+PPDNAGKEEVIAALV N
Sbjct: 181 ARDNVGKHLILTYETAETQDDIMLLRSQVEEDLKKGVAEAVPVPPDNAGKEEVIAALVAN 240

Query: 241 VEAMIKADRKITALKQLQGHVWRTGFSGNELEGVVFDDVPEALERWHALGIKVYIYSSGS 300
           VEAMIKADRKI ALKQLQGH+WRTGFSGNELEG+VFDDVPEALERWHALGIKVYIYSSGS
Sbjct: 241 VEAMIKADRKIAALKQLQGHIWRTGFSGNELEGIVFDDVPEALERWHALGIKVYIYSSGS 300

Query: 301 RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREINSYVEISESVGVDTPSEILFITDVYQ 360
           RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREI SYVEISES+GVDTPSEILFITDVYQ
Sbjct: 301 RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREIRSYVEISESLGVDTPSEILFITDVYQ 360

Query: 361 EAIAAKAAGLQVAISIRPGNSPLPDNHGFQTINSFSEI 391
           EA+AAKAAGLQVAISIRPGN PLPD+HGFQT  SFS+I
Sbjct: 361 EAVAAKAAGLQVAISIRPGNGPLPDDHGFQTATSFSDI 393

BLAST of Tan0004946 vs. ExPASy TrEMBL
Match: A0A1S3C2D2 (probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 1 isoform X2 OS=Cucumis melo OX=3656 GN=LOC103496078 PE=4 SV=1)

HSP 1 Score: 603.2 bits (1554), Expect = 7.7e-169
Identity = 320/398 (80.40%), Postives = 341/398 (85.68%), Query Frame = 0

Query: 1   MAIASALAVDGVNLASTSQAYFE------NKALSTDLCHQVHGLRWVSRARGSIAIKVHD 60
           MA+ASAL + GV LA+TSQAY E       KAL ++L  Q + LR VS    SIAIKVHD
Sbjct: 1   MAMASALPLSGVKLATTSQAYMETKAANDTKALLSELSRQFYTLRCVSGTGSSIAIKVHD 60

Query: 61  HSSPKRQQPIAMSPSGVKKDRMVLEDIFVLSHGGSILYSPPSKPFSHKLPNRWG--PLFM 120
           HS P+RQQ IAMS SGVKK RMV ED ++LS GGSIL+SPP KPFSHKLPN +   PLFM
Sbjct: 61  HSIPRRQQLIAMSLSGVKKRRMVREDTYMLSSGGSILHSPPPKPFSHKLPNCFDSRPLFM 120

Query: 121 KGPIASIKGISRDNVGINISVKAGAQGLDSKTEPLPRCIVLDIEGATTPISFVTDVLFPY 180
           KG I SIKGIS      NISVKAGA+GLD+K +  PRCIVLDIEG TTPISFVTDVLF Y
Sbjct: 121 KGQIGSIKGISS-----NISVKAGARGLDNKNQWFPRCIVLDIEGTTTPISFVTDVLFSY 180

Query: 181 ARDNVGKHLILTYETAETQEDIKLLREQVEDDLEKGVAGAVPIPPDNAGKEEVIAALVGN 240
           ARDNV KHLILTYET ETQ+DIKLLR QVE+DLEKGVAGAVPIPPDNAGKEEVIAA+V N
Sbjct: 181 ARDNVEKHLILTYETGETQDDIKLLRSQVEEDLEKGVAGAVPIPPDNAGKEEVIAAVVAN 240

Query: 241 VEAMIKADRKITALKQLQGHVWRTGFSGNELEGVVFDDVPEALERWHALGIKVYIYSSGS 300
           VE MIKADRKITALKQLQGH+WRTGFSGNELEGVVFDDVPEALERWHA GIK+YIYSSGS
Sbjct: 241 VEGMIKADRKITALKQLQGHIWRTGFSGNELEGVVFDDVPEALERWHASGIKMYIYSSGS 300

Query: 301 RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKREINSYVEISESVGVDTPSEILFITDVYQ 360
           RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKRE +SYVEISESVGVD+PSEILFITDV Q
Sbjct: 301 RLAQRLIFGKTNYGDLRKYLSGFFDTAVGNKRETSSYVEISESVGVDSPSEILFITDVCQ 360

Query: 361 EAIAAKAAGLQVAISIRPGNSPLPDNHGFQTINSFSEI 391
           EA AAKAAGLQVAISIRPGN PLPDNHGFQTI SFSEI
Sbjct: 361 EAKAAKAAGLQVAISIRPGNGPLPDNHGFQTITSFSEI 393

BLAST of Tan0004946 vs. TAIR 10
Match: AT5G53850.2 (haloacid dehalogenase-like hydrolase family protein )

HSP 1 Score: 424.9 bits (1091), Expect = 7.2e-119
Identity = 246/507 (48.52%), Postives = 302/507 (59.57%), Query Frame = 0

Query: 7   LAVDGVNLASTSQAYFENK------ALSTDLCHQVHGLRWVSRARGSIAIKVHDHSSPKR 66
           +AV    +    QAY E K      +L T+LC   +   WVS   GSI +KVHD S PK 
Sbjct: 1   MAVAAAAMIGLPQAYLEGKEVKETSSLVTELCRHFYTQGWVSGTGGSITMKVHDASIPKP 60

Query: 67  QQPIAMSPSGVKKDRMVLEDIFVLSHGGSILYSPPSKPFSHKLP--NRWGPLFMKG---- 126
           +Q I MSPSGV+K+RM  ED+++LS  GSI+ +P  KP+ +K P      PLFMK     
Sbjct: 61  EQLIVMSPSGVQKERMQPEDMYILSANGSIISTPSPKPYPNKPPKCTDCAPLFMKAYEMR 120

Query: 127 -----------------------------PIASIKGISRD-------------------- 186
                                         +  IKGI                       
Sbjct: 121 NAGAVIHSHGMESCLVTMLNPQAKEFRITHMEMIKGIQGHGYYDELVVPIIENTAYENEL 180

Query: 187 --------------------NVGINI---------------------SVKAGAQGLDSKT 246
                               N G+ I                     ++K    GLD+ T
Sbjct: 181 TDSLTKAIEAYPKATAVLVRNHGVYIWGDSWIHAKTQAECYHYLFDAAIKLHQLGLDAAT 240

Query: 247 ---------------------EPLPRCIVLDIEGATTPISFVTDVLFPYARDNVGKHLIL 306
                                E   R IVLDIEG TTPI+FVTDVLFPYAR+NVGKHL L
Sbjct: 241 PDHGPIRRTIHSQIKDSQYEREWPRRWIVLDIEGTTTPITFVTDVLFPYARENVGKHLNL 300

Query: 307 TYETAETQEDIKLLREQVEDDLEKGVAGAVPIPPDNAGKEEVIAALVGNVEAMIKADRKI 366
           TY TAETQEDIKLLR QVE+DL +GV GAVPIP  + GKE+VIAA+V NVEAMI+ADRKI
Sbjct: 301 TYHTAETQEDIKLLRAQVEEDLREGVTGAVPIPHADEGKEKVIAAMVSNVEAMIRADRKI 360

Query: 367 TALKQLQGHVWRTGFSGNELEGVVFDDVPEALERWHALGIKVYIYSSGSRLAQRLIFGKT 391
           TALK+LQGH+WRTGF  +EL+ +VF+DV +ALE+WH+ GIKVYIYSSGSRLAQ+L+FG T
Sbjct: 361 TALKELQGHIWRTGFECDELKAIVFEDVADALEKWHSSGIKVYIYSSGSRLAQKLLFGNT 420

BLAST of Tan0004946 vs. TAIR 10
Match: AT5G53850.1 (haloacid dehalogenase-like hydrolase family protein )

HSP 1 Score: 256.9 bits (655), Expect = 2.6e-68
Identity = 168/401 (41.90%), Postives = 206/401 (51.37%), Query Frame = 0

Query: 7   LAVDGVNLASTSQAYFENK------ALSTDLCHQVHGLRWVSRARGSIAIKVHDHSSPKR 66
           +AV    +    QAY E K      +L T+LC   +   WVS   GSI +KVHD S PK 
Sbjct: 1   MAVAAAAMIGLPQAYLEGKEVKETSSLVTELCRHFYTQGWVSGTGGSITMKVHDASIPKP 60

Query: 67  QQPIAMSPSGVKKDRMVLEDIFVLSHGGSILYSPPSKPFSHKLP--NRWGPLFMKG---- 126
           +Q I MSPSGV+K+RM  ED+++LS  GSI+ +P  KP+ +K P      PLFMK     
Sbjct: 61  EQLIVMSPSGVQKERMQPEDMYILSANGSIISTPSPKPYPNKPPKCTDCAPLFMKAYEMR 120

Query: 127 -----------------------------PIASIKGISRD-------------------- 186
                                         +  IKGI                       
Sbjct: 121 NAGAVIHSHGMESCLVTMLNPQAKEFRITHMEMIKGIQGHGYYDELVVPIIENTAYENEL 180

Query: 187 --------------------NVGINI---------------------SVKAGAQGLDSKT 246
                               N G+ I                     ++K    GLD+ T
Sbjct: 181 TDSLTKAIEAYPKATAVLVRNHGVYIWGDSWIHAKTQAECYHYLFDAAIKLHQLGLDAAT 240

Query: 247 ---------------------EPLPRCIVLDIEGATTPISFVTDVLFPYARDNVGKHLIL 285
                                E   R IVLDIEG TTPI+FVTDVLFPYAR+NVGKHL L
Sbjct: 241 PDHGPIRRTIHSQIKDSQYEREWPRRWIVLDIEGTTTPITFVTDVLFPYARENVGKHLNL 300

BLAST of Tan0004946 vs. TAIR 10
Match: AT5G53850.3 (haloacid dehalogenase-like hydrolase family protein )

HSP 1 Score: 251.1 bits (640), Expect = 1.4e-66
Identity = 169/417 (40.53%), Postives = 206/417 (49.40%), Query Frame = 0

Query: 7   LAVDGVNLASTSQAYFENK------ALSTDLCHQVHGLRWVSRARGSIAIKVHDHSSPKR 66
           +AV    +    QAY E K      +L T+LC   +   WVS   GSI +KVHD S PK 
Sbjct: 1   MAVAAAAMIGLPQAYLEGKEVKETSSLVTELCRHFYTQGWVSGTGGSITMKVHDASIPKP 60

Query: 67  QQPIAMSPSGVKKDRMVLEDIFVLSHGGSILYSPPSKPFSHKLP--NRWGPLFMKGPIAS 126
           +Q I MSPSGV+K+RM  ED+++LS  GSI+ +P  KP+ +K P      PLFMK    S
Sbjct: 61  EQLIVMSPSGVQKERMQPEDMYILSANGSIISTPSPKPYPNKPPKCTDCAPLFMKNRRLS 120

Query: 127 -------------------------------------------------IKGISRD---- 186
                                                            IKGI       
Sbjct: 121 LGKRLRKLGYKAYEMRNAGAVIHSHGMESCLVTMLNPQAKEFRITHMEMIKGIQGHGYYD 180

Query: 187 ------------------------------------NVGINI------------------ 246
                                               N G+ I                  
Sbjct: 181 ELVVPIIENTAYENELTDSLTKAIEAYPKATAVLVRNHGVYIWGDSWIHAKTQAECYHYL 240

Query: 247 ---SVKAGAQGLDSKT---------------------EPLPRCIVLDIEGATTPISFVTD 285
              ++K    GLD+ T                     E   R IVLDIEG TTPI+FVTD
Sbjct: 241 FDAAIKLHQLGLDAATPDHGPIRRTIHSQIKDSQYEREWPRRWIVLDIEGTTTPITFVTD 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
E0CSI19.7e-13754.26Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
B9N1F91.4e-13554.07Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
B9SQI72.4e-13553.52Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
E0CTF33.1e-11948.19Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
Q9FN411.0e-11748.52Probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
Match NameE-valueIdentityDescription
XP_038899594.13.5e-17682.66probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
XP_023551888.11.8e-17280.40probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
XP_022923008.15.3e-17280.15probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
XP_022923007.12.0e-17179.90probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
XP_022923004.12.0e-17179.90probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
Match NameE-valueIdentityDescription
A0A6J1E8E32.5e-17280.15probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
A0A6J1EAE79.7e-17279.90probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
A0A6J1E4Z29.7e-17279.90probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
A0A6J1J2461.4e-17079.40probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
A0A1S3C2D27.7e-16980.40probable bifunctional methylthioribulose-1-phosphate dehydratase/enolase-phospha... [more]
Match NameE-valueIdentityDescription
AT5G53850.27.2e-11948.52haloacid dehalogenase-like hydrolase family protein [more]
AT5G53850.12.6e-6841.90haloacid dehalogenase-like hydrolase family protein [more]
AT5G53850.31.4e-6640.53haloacid dehalogenase-like hydrolase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036409Class II aldolase/adducin N-terminal domain superfamilyGENE3D3.40.225.10coord: 23..112
e-value: 2.2E-8
score: 35.9
NoneNo IPR availableGENE3D1.10.720.60coord: 162..257
e-value: 3.3E-29
score: 103.7
NoneNo IPR availablePANTHERPTHR10640:SF8BIFUNCTIONAL METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASE/ENOLASE-PHOSPHATASE E1-RELATEDcoord: 113..321
NoneNo IPR availablePANTHERPTHR10640:SF8BIFUNCTIONAL METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASE/ENOLASE-PHOSPHATASE E1-RELATEDcoord: 5..105
IPR023214HAD superfamilyGENE3D3.40.50.1000coord: 258..321
e-value: 8.9E-19
score: 69.6
IPR023943Enolase-phosphatase E1TIGRFAMTIGR01691TIGR01691coord: 149..321
e-value: 5.0E-46
score: 154.9
IPR017714Methylthioribulose-1-phosphate dehydratasePANTHERPTHR10640METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASEcoord: 5..105
IPR017714Methylthioribulose-1-phosphate dehydratasePANTHERPTHR10640METHYLTHIORIBULOSE-1-PHOSPHATE DEHYDRATASEcoord: 113..321
IPR036412HAD-like superfamilySUPERFAMILY56784HAD-likecoord: 149..319

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0004946.1Tan0004946.1mRNA
Tan0004946.2Tan0004946.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016311 dephosphorylation
biological_process GO:0019509 L-methionine salvage from methylthioadenosine
cellular_component GO:0005737 cytoplasm
cellular_component GO:0016020 membrane
molecular_function GO:0043874 acireductone synthase activity
molecular_function GO:0000287 magnesium ion binding
molecular_function GO:0046872 metal ion binding