Tan0004770 (gene) Snake gourd v1

Overview


Name	Tan0004770
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	O-methyltransferase family protein
Location	LG02: 5834624 .. 5836995 (+)
RNA-Seq Expression	Tan0004770
Synteny	Tan0004770

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACTTAGCCACAAAGAATCTTCATCAAGGAAGGAAGAAGATGATCATGCCATCCTCCAGATGTGGAAATACATATATGGTTTCACTGAAATAGCAGCAATAAAATGTGCTATACAACTTAAAATTGCTGATAGTATTGAAGGTTATGGAAGTCCAATGACACTGTCCCAACTATCATCCACTTTGAATTGTTCTTCGTCGCTTCTGTACCGCATCTTGAGATTCCTCATTCATCGTCAAATTTTTAAAGAAGAAACCATAGATGAAACTGAAAACTTGAGAGGTTATGCCCAAACACCTTTGTCTCGCTTGCTCACCACCAACAGTGAGAACAGTATGATTCCCTTATTTTTATTAGAAAGTAGCCCAGTGATGCTTGCAGCATGGCTAAACTTGAGTGTGCATGTCAAAAATAGTGAGACTGTGCCATTTGAGGTTGCTCATGGCATGGACTTGTGGAGTTATGTTGCAGAAAATTCAAAGCACAATATACTATTTAATGAGGCTATGGCTTGTTCAGCTCGTGTGATTACTTGTGCCATACTTGAATTAGGTTGTGGTGGAGATATGTTTAATGGAGTTAGAACTTTGGTCGATGTGGGAGGAGGAAATGGAACTACTTTGAGCATTTTTCTTAAGGCTTGCCCATGGATTCAAGGTGGTATTAATTTTGATCTTCCACATGTTGTTTCTGCTTCACCACAATACAAAAATGTGAAGCTAGCATGTAGCTGGTGACATGTTCAACTTTGTCCCAAAAGCTGATGTTGCTCTCCTCCAGGTTGGTTTTATAGATATTATATATGTTTGTGTTTGAGGTAAAATTTCATATTTTGGTTCATATATGATATCTTCGAAAACTTAAAGAGGAAAGAGGACAAGTATATGCTTGAAATATTTTTTTTATAGTTAAGGCTTCGTTTCATAAACATTTAGATCTTGTTTGATAACCATTTTGTTTTTTGTTTTTTATTTTTGAAAATTAAGCCTATAAATACTATTTTCACTTGTGATTTTTTATGTTTTCTTATCTATTTTTTATATGTGTTTTCAAAAAATCAAGCCAAGTTTTGAAAACTAAAAAAAGTAATTTCCAAAAACTTCTTTTTGTTTTTTAAATTTAGCTAAGAATTCAAGTGTTTTCTTATGAAAGATGAGAACCATTGTAGGGAAGATTAAAATCATCATGGTAGGAAAATGGTAAGAAAACAAATCTAATTTTCAAAAACTAAAAACCAAATGGTTATCAAATGGGGTCTTTTTTTTTTGTTTTTGAAAATTAAGCATATAAATATGATCTCCACCTATGATTTTCTATATTTTCTTATCTACTTTTTAGATGTGTAATTTTGCAAACTAAAAAAAGTAGTGTCCAAAAACTTTTTTTTTTTTAATCTAGCTCAGAATTCAAGTGATTTCTTAAAAAAGATGGGAACCATGGTAGGGAAGATGAAAATCATTGTAGAGAAATTGTGAAAAAATGAGTATAATTTTCAAAAACCAAAAATCAGATAGTTATAAAAGCGGGTCTAAGTTTTCTAAATTAGTACACAGTATTAATTCTATTTCTAAAAATGAAAACAGTTTTTTTGGGTTGAAAAGTACTTGTTTAGCAGTATAATTTTCAAAATAAGAAACAGGAGAGAAACAAACAAAATATTTTGTCGAGTGTATTCTTATCTTTTCTATTTGGGGGGATTCTATTTTGTTATCTTTACAATTGCATTATTTGTAATGTGTATATAATCATTATTTATTTTTTTTAACTTGATTTAAATATATTATACTTTTAAATTTATATGATGGAATTGAAAAATAAATGAAAATATGAAAAACAAAAGTATTATTTCAATCGGATTTTAAATTTAAATAATAAAAAAAAACAATCATAAAAGATCGATCAATGAAACAATTCTTAGAAATAATTTTTAAGAAATCTAACCAAACAGTTTTTTACTTTCAACCACATAAGCACCCTAATTTGCTTAATTGATCTCTTCAAGTTCTAGCAATAACTGTACAAATTAGTGACAATTGTTGAAGCTAAATCGCAGTGGGTTCTACATGATTGGAATGATGATGAATGTATTAAAATTTTGAAGAATTGCAAGGAAGCCATTCCAGAAGATATAGGAAAAGTGATAATTATAGAAATAGTAATGGATGAAAAAGAAGACAATAAGTTCTTAGATGTCGGTTTGATGCTTGACCTCGGGATGATGGCTCATACCAACAAAGGCAAGGAAAGAACATGTGAAGAATGGGCATATATTATCAACAAAGCTGGTTTTAGTAATTCAATGTTTCCCTTGAAGAAGACGAGAAAAAAAAAATGGATTTGTTATCCTTGGATGACATACATTCATTAG

mRNA sequence

ATGGAACTTAGCCACAAAGAATCTTCATCAAGGAAGGAAGAAGATGATCATGCCATCCTCCAGATGTGGAAATACATATATGGTTTCACTGAAATAGCAGCAATAAAATGTGCTATACAACTTAAAATTGCTGATAGTATTGAAGGTTATGGAAGTCCAATGACACTGTCCCAACTATCATCCACTTTGAATTGTTCTTCGTCGCTTCTGTACCGCATCTTGAGATTCCTCATTCATCGTCAAATTTTTAAAGAAGAAACCATAGATGAAACTGAAAACTTGAGAGGTTATGCCCAAACACCTTTGTCTCGCTTGCTCACCACCAACAGTGAGAACAGTATGATTCCCTTATTTTTATTAGAAAGTAGCCCAGTGATGCTTGCAGCATGGCTAAACTTGAGTGTGCATGTCAAAAATAGTGAGACTGTGCCATTTGAGGTTGCTCATGGCATGGACTTGTGGAGTTATGTTGCAGAAAATTCAAAGCACAATATACTATTTAATGAGGCTATGGCTTGTTCAGCTCGTGTGATTACTTGTGCCATACTTGAATTAGGTTGTGGTGGAGATATGTTTAATGGAGTTAGAACTTTGGTCGATGTGGGAGGAGGAAATGGAACTACTTTGAGCATTTTTCTTAAGGCTTGCCCATGGATTCAAGGTGGTATTAATTTTGATCTTCCACATGTTCATGTAGCTGGTGACATGTTCAACTTTGTCCCAAAAGCTGATGTTGCTCTCCTCCAGTGGGTTCTACATGATTGGAATGATGATGAATGTATTAAAATTTTGAAGAATTGCAAGGAAGCCATTCCAGAAGATATAGGAAAAGTGATAATTATAGAAATAGTAATGGATGAAAAAGAAGACAATAAGTTCTTAGATGTCGGTTTGATGCTTGACCTCGGGATGATGGCTCATACCAACAAAGGCAAGGAAAGAACATGTGAAGAATGGGCATATATTATCAACAAAGCTGGTTTTAGTAATTCAATGTTTCCCTTGAAGAAGACGAGAAAAAAAAAATGGATTTGTTATCCTTGGATGACATACATTCATTAG

Coding sequence (CDS)

ATGGAACTTAGCCACAAAGAATCTTCATCAAGGAAGGAAGAAGATGATCATGCCATCCTCCAGATGTGGAAATACATATATGGTTTCACTGAAATAGCAGCAATAAAATGTGCTATACAACTTAAAATTGCTGATAGTATTGAAGGTTATGGAAGTCCAATGACACTGTCCCAACTATCATCCACTTTGAATTGTTCTTCGTCGCTTCTGTACCGCATCTTGAGATTCCTCATTCATCGTCAAATTTTTAAAGAAGAAACCATAGATGAAACTGAAAACTTGAGAGGTTATGCCCAAACACCTTTGTCTCGCTTGCTCACCACCAACAGTGAGAACAGTATGATTCCCTTATTTTTATTAGAAAGTAGCCCAGTGATGCTTGCAGCATGGCTAAACTTGAGTGTGCATGTCAAAAATAGTGAGACTGTGCCATTTGAGGTTGCTCATGGCATGGACTTGTGGAGTTATGTTGCAGAAAATTCAAAGCACAATATACTATTTAATGAGGCTATGGCTTGTTCAGCTCGTGTGATTACTTGTGCCATACTTGAATTAGGTTGTGGTGGAGATATGTTTAATGGAGTTAGAACTTTGGTCGATGTGGGAGGAGGAAATGGAACTACTTTGAGCATTTTTCTTAAGGCTTGCCCATGGATTCAAGGTGGTATTAATTTTGATCTTCCACATGTTCATGTAGCTGGTGACATGTTCAACTTTGTCCCAAAAGCTGATGTTGCTCTCCTCCAGTGGGTTCTACATGATTGGAATGATGATGAATGTATTAAAATTTTGAAGAATTGCAAGGAAGCCATTCCAGAAGATATAGGAAAAGTGATAATTATAGAAATAGTAATGGATGAAAAAGAAGACAATAAGTTCTTAGATGTCGGTTTGATGCTTGACCTCGGGATGATGGCTCATACCAACAAAGGCAAGGAAAGAACATGTGAAGAATGGGCATATATTATCAACAAAGCTGGTTTTAGTAATTCAATGTTTCCCTTGAAGAAGACGAGAAAAAAAAAATGGATTTGTTATCCTTGGATGACATACATTCATTAG

Protein sequence

MELSHKESSSRKEEDDHAILQMWKYIYGFTEIAAIKCAIQLKIADSIEGYGSPMTLSQLSSTLNCSSSLLYRILRFLIHRQIFKEETIDETENLRGYAQTPLSRLLTTNSENSMIPLFLLESSPVMLAAWLNLSVHVKNSETVPFEVAHGMDLWSYVAENSKHNILFNEAMACSARVITCAILELGCGGDMFNGVRTLVDVGGGNGTTLSIFLKACPWIQGGINFDLPHVHVAGDMFNFVPKADVALLQWVLHDWNDDECIKILKNCKEAIPEDIGKVIIIEIVMDEKEDNKFLDVGLMLDLGMMAHTNKGKERTCEEWAYIINKAGFSNSMFPLKKTRKKKWICYPWMTYIH

Homology

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BLAST of Tan0004770 vs. ExPASy Swiss-Prot
Match: Q9T003 (Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1 SV=1)

HSP 1 Score: 295.0 bits (754), Expect = 1.1e-78
Identity = 164/359 (45.68%), Postives = 226/359 (62.95%), Query Frame = 0


Query: 1   MELSHKESSSRK--EEDDHAILQMWKYIYGFTEIAAIKCAIQLKIADSIEGYGS--PMTL 60
           ++ +  E + RK  +E+  A L +WKY++GF +IAA KCAI LKI ++IE + S  P+TL
Sbjct: 10  LDRNKMEDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTL 69

Query: 61  SQLSSTLNCSSSLLYRILRFLIHRQIFKEETIDETENL-RGYAQTPLSR--LLTTNSENS 120
           ++LSS ++ S S L RI+RFL+H+ IFKE  I   + L  GY  TPLSR  ++T     S
Sbjct: 70  AELSSAVSASPSHLRRIMRFLVHQGIFKE--IPTKDGLATGYVNTPLSRRLMITRRDGKS 129

Query: 121 MIPLFLLESSPVMLAAWLNL----SVHVKNSETVPFEVAHGMDLWSYVAENSKHNILFNE 180
           + P  L E++P MLA WL L    S  V  S   PF+  HG D+WS+  +N   + + NE
Sbjct: 130 LAPFVLFETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINE 189

Query: 181 AMACSARVITCAILELGCGGDMFNGVRTLVDVGGGNGTTLSIFLKACPWIQGGINFDLPH 240
           AMAC AR +   +   G    +F+GV T+VDVGGG G T+ + +K  PWI+ G NFDLPH
Sbjct: 190 AMACDARRVVPRV--AGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIK-GFNFDLPH 249

Query: 241 V-----------HVAGDMFNFVPKADVALLQWVLHDWNDDECIKILKNCKEAIPEDIGKV 300
           V           +V GDMF+ +P  D   ++WVLHDW D +CIKILKNCKEA+P +IGKV
Sbjct: 250 VIEVAEVLDGVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKV 309

Query: 301 IIIEIVMDE--------KEDNKFLDVGLMLDLGMMAHTNKGKERTCEEWAYIINKAGFS 330
           +I+E V+ E        + D K   V LMLD+ MMAHT+ GKERT +EW +++ +AGF+
Sbjct: 310 LIVESVIGENKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFA 363

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BLAST of Tan0004770 vs. ExPASy Swiss-Prot
Match: Q7XB10 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2 OS=Papaver somniferum OX=3469 GN=4'OMT2 PE=1 SV=1)

HSP 1 Score: 240.0 bits (611), Expect = 4.2e-62
Identity = 130/349 (37.25%), Postives = 209/349 (59.89%), Query Frame = 0


Query: 3   LSHKESSSRKEEDDHAILQMWKYIYGFTEIAAIKCAIQLKIADSIEGYGSPMTLSQLSST 62
           L  K +++ +E       Q+W  IYGF +   ++CA+++ IAD I+     +TL+QL++ 
Sbjct: 4   LDAKPAAATQEVSIKDQAQLWNIIYGFADSLVLRCAVEIGIADIIKNNDGAITLAQLAAK 63

Query: 63  L---NCSSSLLYRILRFLIHRQIFKEETIDETENLRGYAQTPLSRLLTTNSENSMIPLFL 122
           L   N SS  LYR++R+L+H  I ++ET +     + Y+  P+  LL  ++E SM+P+ L
Sbjct: 64  LPITNVSSDYLYRMVRYLVHLNIIEQETCNGGVE-KVYSLKPVGTLLLRDAERSMVPMIL 123

Query: 123 LESSPVMLAAWLNLSVHVKNSETVPFEVAHGMDLWSYVAENSKHNILFNEAMACSARVIT 182
             +    + +W  +   + N  T  FE   GMD+W Y+  N   + LFNE MA   R++T
Sbjct: 124 GMTQKDFMVSWHFMKEGLGNGSTTAFEKGMGMDIWKYLEGNPDQSQLFNEGMAGETRLLT 183

Query: 183 CAILELGCGGDMFNGVRTLVDVGGGNGTTLSIFLKACPWIQGGINFDLPHV--------- 242
             ++E  C  D F G+ +LVD+GGGNGTT+    +A P I+  + +DLPHV         
Sbjct: 184 KTLIE-DC-RDTFQGLDSLVDIGGGNGTTIKAIYEAFPHIKCTL-YDLPHVVANSHDLPN 243

Query: 243 --HVAGDMFNFVPKADVALLQWVLHDWNDDECIKILKNCKEAIPEDIGKVIIIEIVMDEK 302
              V GDMF  VP A   LL+ +LHDW D+EC+ ILK CKEAIP++ GKVII+++ ++E+
Sbjct: 244 IEKVPGDMFKSVPSAQAILLKLILHDWTDEECVNILKKCKEAIPKETGKVIIVDVALEEE 303

Query: 303 EDNKFLDVGLMLDLGMMAHTNKGKERTCEEWAYIINKAGF-SNSMFPLK 337
            +++     L+LD+ M+ +T  G+ERT ++W  ++ +AGF S+ + P++
Sbjct: 304 SNHELTKTRLILDIDMLVNTG-GRERTADDWENLLKRAGFRSHKIRPIR 347

[Close]

BLAST of Tan0004770 vs. ExPASy Swiss-Prot
Match: Q9LEL6 ((RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1)

HSP 1 Score: 238.8 bits (608), Expect = 9.3e-62
Identity = 136/331 (41.09%), Postives = 203/331 (61.33%), Query Frame = 0


Query: 12  KEEDDHAILQMWKYIYGFTEIAAIKCAIQLKIADSIEGYGSPMTLSQLSSTL---NCSSS 71
           K+++  +  ++W +IYGF E   +KCA+QL +A+ I   G+ MTLS+LSS L     +  
Sbjct: 4   KKDNLSSQAKLWNFIYGFAESLVLKCAVQLDLANIIHNSGTSMTLSELSSRLPSQPVNED 63

Query: 72  LLYRILRFLIHRQIFKEETIDETENLRGYAQTPLSRLLTTNSENSMIPLFLLESSPVMLA 131
            LYR++R+L+H ++F + +ID    LR Y   P ++ L    +  M+   L  +    +A
Sbjct: 64  ALYRVMRYLVHMKLFTKASID--GELR-YGLAPPAKYLVKGWDKCMVGSILAITDKDFMA 123

Query: 132 AWLNLSVHVKNSETVPFEVAHGMDLWSYVAENSKHNILFNEAMACSARVITCAILELGCG 191
            W  L   +       FE A G ++W Y+AE+ + N LFNEAMA  +R+I  A+++  C 
Sbjct: 124 PWHYLKDGLSGESGTAFEKALGTNIWGYMAEHPEKNQLFNEAMANDSRLIMSALVK-EC- 183

Query: 192 GDMFNGVRTLVDVGGGNGTTLSIFLKACPWIQGGINFDLPHV-----------HVAGDMF 251
           G++FNG+ TLVDVGGG GT +     A P I+  + +DLPHV            VAGDMF
Sbjct: 184 GNIFNGITTLVDVGGGTGTAVRNIANAFPHIKCTV-YDLPHVIADSPGYSEVHCVAGDMF 243

Query: 252 NFVPKADVALLQWVLHDWNDDECIKILKNCKEAIPEDIGKVIIIEIVMDEKEDNKFLDVG 311
            F+PKAD  +++ +LHDW+D ECI+ILK CKEA+P   GKVII++IV++ + ++ +  + 
Sbjct: 244 KFIPKADAIMMKCILHDWDDKECIEILKRCKEAVPVKGGKVIIVDIVLNVQSEHPYTKMR 303

Query: 312 LMLDLGMMAHTNKGKERTCEEWAYIINKAGF 329
           L LDL MM +T  GKERT EEW  +I+ AG+
Sbjct: 304 LTLDLDMMLNTG-GKERTEEEWKKLIHDAGY 327

[Close]

BLAST of Tan0004770 vs. ExPASy Swiss-Prot
Match: Q5C9L7 ((RS)-norcoclaurine 6-O-methyltransferase OS=Thalictrum flavum subsp. glaucum OX=150095 PE=1 SV=1)

HSP 1 Score: 236.9 bits (603), Expect = 3.5e-61
Identity = 138/344 (40.12%), Postives = 206/344 (59.88%), Query Frame = 0


Query: 1   MELSHKESSSRKEEDDHAILQMWKYIYGFTEIAAIKCAIQLKIADSIEGYGSPMTLSQLS 60
           ME+ +KE+ S +        ++W +IYGF +   +K A+QL +A+ I  +GSPMTLS+LS
Sbjct: 1   MEMINKENLSSQ-------AKLWNFIYGFADSLVLKSAVQLDLANIIHNHGSPMTLSELS 60

Query: 61  STL---NCSSSLLYRILRFLIHRQIFKEETIDETENLRGYAQTPLSRLLTTNSENSMIPL 120
             L     +   LYR+LR+L+H ++F + +ID    LR Y   P ++ L    +  M+  
Sbjct: 61  LHLPSQPVNQDALYRVLRYLVHMKLFTKSSID--GELR-YGLAPPAKFLVKGWDKCMLGA 120

Query: 121 FLLESSPVMLAAWLNLSVHVKN--SETVPFEVAHGMDLWSYVAENSKHNILFNEAMACSA 180
            L  +    +A W  L   + N  S +  FE A G ++W Y+AE+ + N LFNE MA   
Sbjct: 121 ILTITDKDFMAPWHYLKEGILNDGSTSTAFEKALGTNIWDYMAEHPEKNQLFNEGMANDT 180

Query: 181 RVITCAILELGCGGDMFNGVRTLVDVGGGNGTTLSIFLKACPWIQGGINFDLPHV----- 240
           R+I  A+++  C   MF+G+ T+VDVGGG GT +    KA P I+  + +DLPHV     
Sbjct: 181 RLIMSALVK-EC-SSMFDGITTIVDVGGGTGTAVRNIAKAFPHIKCTV-YDLPHVIADSP 240

Query: 241 ------HVAGDMFNFVPKADVALLQWVLHDWNDDECIKILKNCKEAIPEDIGKVIIIEIV 300
                  + GDMF ++P AD  +++ +LHDW+D ECI+ILK CK+A+P D GKVIII+I+
Sbjct: 241 GYTEINSIQGDMFKYIPNADAIMMKCILHDWDDKECIEILKRCKDAVPRDGGKVIIIDII 300

Query: 301 MDEKEDNKFLDVGLMLDLGMMAHTNKGKERTCEEWAYIINKAGF 329
           +D K ++ +  + L LDL MM +T  GKERT EEW  +I+ AG+
Sbjct: 301 LDVKSEHPYTKMRLTLDLDMMLNTG-GKERTEEEWKKLIHDAGY 330

[Close]

BLAST of Tan0004770 vs. ExPASy Swiss-Prot
Match: Q9LEL5 (3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1)

HSP 1 Score: 236.1 bits (601), Expect = 6.0e-61
Identity = 131/331 (39.58%), Postives = 197/331 (59.52%), Query Frame = 0


Query: 15  DDHAILQMWKYIYGFTEIAAIKCAIQLKIADSIEGYGSPMTLSQLSSTL-----NCSSSL 74
           D  A   +WK IYGF +   ++CA++L I D I+    PM L+ L+S L     NC +  
Sbjct: 11  DIKAQAHVWKIIYGFADSLVLRCAVELGIVDIIDNNNQPMALADLASKLPVSDVNCDN-- 70

Query: 75  LYRILRFLIHRQIFKEETIDETENLRGYAQTPLSRLLTTNSENSMIPLFLLESSPVMLAA 134
           LYRILR+L+  +I + E  D+ +  + YA  P++ LL+ N++ SM+P+ L  +    +  
Sbjct: 71  LYRILRYLVKMEILRVEKSDDGQ--KKYALEPIATLLSRNAKRSMVPMILGMTQKDFMTP 130

Query: 135 WLNLSVHVKNSETVPFEVAHGMDLWSYVAENSKHNILFNEAMACSARVITCAILELGCGG 194
           W ++   + ++ T  FE A GM +W Y+  +   + LFNE MA   R++T ++  +    
Sbjct: 131 WHSMKDGLSDNGTA-FEKAMGMTIWEYLEGHPDQSQLFNEGMAGETRLLTSSL--ISGSR 190

Query: 195 DMFNGVRTLVDVGGGNGTTLSIFLKACPWIQGGINFDLPHV-----------HVAGDMFN 254
           DMF G+ +LVDVGGGNGTT+     A P I+  + FDLPHV            + GDMF 
Sbjct: 191 DMFQGIDSLVDVGGGNGTTVKAISDAFPHIKCTL-FDLPHVIANSYDLPNIERIGGDMFK 250

Query: 255 FVPKADVALLQWVLHDWNDDECIKILKNCKEAIPEDIGKVIIIEIVMDEKEDNKFLDVGL 314
            VP A   +L+ +LHDWND++ IKILK C+ A+P+D GKVII+++ +DE+ D++     L
Sbjct: 251 SVPSAQAIILKLILHDWNDEDSIKILKQCRNAVPKDGGKVIIVDVALDEESDHELSSTRL 310

Query: 315 MLDLGMMAHTNKGKERTCEEWAYIINKAGFS 330
           +LD+ M+ +T  GKERT E W  I+  AGFS
Sbjct: 311 ILDIDMLVNTG-GKERTKEVWEKIVKSAGFS 332

[Close]

BLAST of Tan0004770 vs. NCBI nr
Match: XP_038888809.1 (acetylserotonin O-methyltransferase-like [Benincasa hispida])

HSP 1 Score: 479.2 bits (1232), Expect = 3.1e-131
Identity = 240/341 (70.38%), Postives = 280/341 (82.11%), Query Frame = 0


Query: 1   MELSHKESSSRKEEDDHAILQMWKYIYGFTEIAAIKCAIQLKIADSIEGYGSPMTLSQLS 60
           ME+ HK+ SS+KE+D  AI+QMWKY++ FTE+AAIKC I LKIAD IE YGSPMTLS LS
Sbjct: 1   MEVEHKKCSSKKEDDGQAIIQMWKYVFSFTEMAAIKCVIDLKIADIIESYGSPMTLSNLS 60

Query: 61  STLNCSSSLLYRILRFLIHRQIFKEETIDETENLRGYAQTPLSRLLTTNSENSMIPLFLL 120
           STLNCSSSLLYRILRFLIHR IFKEETI E + +  Y+QTPLSRLL TN EN+M PL LL
Sbjct: 61  STLNCSSSLLYRILRFLIHRGIFKEETIGENQTV--YSQTPLSRLLATNVENTMAPLLLL 120

Query: 121 ESSPVMLAAWLNLSVHVKNSETVPFEVAHGMDLWSYVAENSKHNILFNEAMACSARVITC 180
           E+SPVMLAAW NLS H+KNSET+PFE+AHGMD WSY   N +HN+LFNEAMAC A+VI  
Sbjct: 121 ETSPVMLAAWPNLSAHLKNSETLPFEIAHGMDFWSYAEANPEHNVLFNEAMACYAKVIAS 180

Query: 181 AILELGCGGDMFNGVRTLVDVGGGNGTTLSIFLKACPWIQGGINFDLPHV---------- 240
           AILE GC GD+F+GV  LVDVGGGNG+TLSI +KACPW++ GINFDLPHV          
Sbjct: 181 AILE-GC-GDVFDGVGCLVDVGGGNGSTLSILVKACPWMK-GINFDLPHVVCAAPQYENV 240

Query: 241 -HVAGDMFNFVPKADVALLQWVLHDWNDDECIKILKNCKEAIPEDIGKVIIIEIVMDEK- 300
            HVAG+MF+FVP+ADVA L+W+LHDWND+ECIKILK CKE IP+  GKVII+E +++EK 
Sbjct: 241 QHVAGNMFDFVPEADVAFLKWILHDWNDEECIKILKKCKEVIPKSGGKVIIVEAIVEEKG 300

Query: 301 EDNKFLDVGLMLDLGMMAHTNKGKERTCEEWAYIINKAGFS 330
           E NK  DVGLM D+ MMAHTNKGKERT EEWA++IN AGF+
Sbjct: 301 EKNKKSDVGLMFDVAMMAHTNKGKERTIEEWAFVINAAGFT 336

[Close]

BLAST of Tan0004770 vs. NCBI nr
Match: XP_038887538.1 (acetylserotonin O-methyltransferase-like isoform X1 [Benincasa hispida])

HSP 1 Score: 471.5 bits (1212), Expect = 6.5e-129
Identity = 240/341 (70.38%), Postives = 277/341 (81.23%), Query Frame = 0


Query: 1   MELSHKESSSRKEEDDHAILQMWKYIYGFTEIAAIKCAIQLKIADSIEGYGSPMTLSQLS 60
           ME+ HKE SS+ EED  AI+QMW+YI+ FTE+AAIKC I LKIAD IE YGSPMTLS LS
Sbjct: 1   MEVKHKECSSKTEEDGQAIVQMWRYIFRFTEMAAIKCVIDLKIADIIESYGSPMTLSHLS 60

Query: 61  STLNCSSSLLYRILRFLIHRQIFKEETIDETENLRGYAQTPLSRLLTTNSENSMIPLFLL 120
           S LNCSSSLL+RILRFLIHR IFKEETI   EN   Y+QTPLSRLL TN E++M PL LL
Sbjct: 61  SALNCSSSLLHRILRFLIHRGIFKEETIG--ENQTAYSQTPLSRLLATNVESTMAPLLLL 120

Query: 121 ESSPVMLAAWLNLSVHVKNSETVPFEVAHGMDLWSYVAENSKHNILFNEAMACSARVITC 180
           E+SPVMLAAW NLS H+KNSET PFE+AHG DLWSY   N +HN+LFNEAMAC A+VI  
Sbjct: 121 ETSPVMLAAWPNLSAHLKNSETFPFEIAHGKDLWSYAEANPEHNVLFNEAMACYAKVIAF 180

Query: 181 AILELGCGGDMFNGVRTLVDVGGGNGTTLSIFLKACPWIQGGINFDLPHV---------- 240
           AILE GC GD+F+GV  LVDVGGGNG+TLSI + ACPW++ GINFDLPHV          
Sbjct: 181 AILE-GC-GDIFDGVGCLVDVGGGNGSTLSILVNACPWMK-GINFDLPHVVCASPQYENV 240

Query: 241 -HVAGDMFNFVPKADVALLQWVLHDWNDDECIKILKNCKEAIPEDIGKVIIIEIVMDEKE 300
            HVAG+MF+FVPKADVA L+W+LHDWND+ECIKILK CKEAIP+  GKVII+E +++EK 
Sbjct: 241 QHVAGNMFDFVPKADVAFLKWILHDWNDEECIKILKRCKEAIPKSGGKVIIVEAIIEEKS 300

Query: 301 DNKFL-DVGLMLDLGMMAHTNKGKERTCEEWAYIINKAGFS 330
           +NK + DVGLM DL MMAHTNKGKERT EEWA++IN AGF+
Sbjct: 301 ENKKVSDVGLMFDLVMMAHTNKGKERTIEEWAFVINAAGFT 336

[Close]

BLAST of Tan0004770 vs. NCBI nr
Match: XP_038887354.1 (acetylserotonin O-methyltransferase-like isoform X1 [Benincasa hispida])

HSP 1 Score: 470.7 bits (1210), Expect = 1.1e-128
Identity = 239/341 (70.09%), Postives = 277/341 (81.23%), Query Frame = 0


Query: 1   MELSHKESSSRKEEDDHAILQMWKYIYGFTEIAAIKCAIQLKIADSIEGYGSPMTLSQLS 60
           ME+ HKE SS+ EED  AI+QMW+YI+ FTE+AAIKC I LKIAD IE YGSPMTLS LS
Sbjct: 1   MEVKHKECSSKTEEDGQAIVQMWRYIFRFTEMAAIKCVIDLKIADIIESYGSPMTLSHLS 60

Query: 61  STLNCSSSLLYRILRFLIHRQIFKEETIDETENLRGYAQTPLSRLLTTNSENSMIPLFLL 120
           S LNCSSS+L+RILRFLIHR IFKEETI   EN   Y+QTPLSRLL TN E++M PL LL
Sbjct: 61  SALNCSSSILHRILRFLIHRGIFKEETIG--ENQTAYSQTPLSRLLATNVESTMAPLLLL 120

Query: 121 ESSPVMLAAWLNLSVHVKNSETVPFEVAHGMDLWSYVAENSKHNILFNEAMACSARVITC 180
           E+SPVMLAAW NLS H+KNSET PFE+AHG DLWSY   N +HN+LFNEAMAC A+VI  
Sbjct: 121 ETSPVMLAAWPNLSAHLKNSETFPFEIAHGKDLWSYAEANPEHNVLFNEAMACYAKVIAF 180

Query: 181 AILELGCGGDMFNGVRTLVDVGGGNGTTLSIFLKACPWIQGGINFDLPHV---------- 240
           AILE GC GD+F+GV  LVDVGGGNG+TLSI + ACPW++ GINFDLPHV          
Sbjct: 181 AILE-GC-GDIFDGVGCLVDVGGGNGSTLSILVNACPWMK-GINFDLPHVVCASPQYENV 240

Query: 241 -HVAGDMFNFVPKADVALLQWVLHDWNDDECIKILKNCKEAIPEDIGKVIIIEIVMDEKE 300
            HVAG+MF+FVPKADVA L+W+LHDWND+ECIKILK CKEAIP+  GKVII+E +++EK 
Sbjct: 241 QHVAGNMFDFVPKADVAFLKWILHDWNDEECIKILKRCKEAIPKSGGKVIIVEAIIEEKS 300

Query: 301 DNKFL-DVGLMLDLGMMAHTNKGKERTCEEWAYIINKAGFS 330
           +NK + DVGLM DL MMAHTNKGKERT EEWA++IN AGF+
Sbjct: 301 ENKKVSDVGLMFDLVMMAHTNKGKERTIEEWAFVINAAGFT 336

[Close]

BLAST of Tan0004770 vs. NCBI nr
Match: KAA0040349.1 ((RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo var. makuwa] >TYK23355.1 (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo var. makuwa])

HSP 1 Score: 468.0 bits (1203), Expect = 7.2e-128
Identity = 240/363 (66.12%), Postives = 282/363 (77.69%), Query Frame = 0


Query: 1   MELSHKESSSRKEEDDHAILQMWKYIYGFTEIAAIKCAIQLKIADSIEGYGSPMTLSQLS 60
           ME+ HKESSS KEED  AI+QMW+YI+ FTE+AAIKCAI LKIAD IE YGSP+TLSQLS
Sbjct: 1   MEVKHKESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCAIDLKIADIIESYGSPVTLSQLS 60

Query: 61  STLNCSSSLLYRILRFLIHRQIFKEETIDETENLRGYAQTPLSRLLTTNSENSMIPLFLL 120
           STLNCSSSLLYRILRFLIHR IFK ETID  EN  GY+QTP+SRLL TN ENSM PL LL
Sbjct: 61  STLNCSSSLLYRILRFLIHRGIFKRETID--ENQIGYSQTPMSRLLATNVENSMAPLLLL 120

Query: 121 ESSPVMLAAWLNLSVHVKNSETVPFEVAHGMDLWSYVAENSKHNILFNEAMACSARVITC 180
           E+SPVMLA W +LS H+KNS+T PFE+AHG DLW+Y   N +HN+LFNEAMACSA+VI  
Sbjct: 121 ETSPVMLAPWQHLSAHLKNSDTSPFEIAHGKDLWNYAEANHEHNLLFNEAMACSAKVIVS 180

Query: 181 AILELGCGGDMFNGVRTLVDVGGGNGTTLSIFLKACPWIQGGINFDLPHV---------- 240
           AI+E GC GD+F+GV  LVDVGGGNG+TL+I +K CPW++ GINFDLPHV          
Sbjct: 181 AIIE-GC-GDVFDGVGCLVDVGGGNGSTLNILVKVCPWMK-GINFDLPHVVCASPQYENV 240

Query: 241 -HVAGDMFNFVPKADVALLQWVLHDWNDDECIKILKNCKEAIPEDIGKVIIIEIVM---- 300
            HVAG+MF+FVP ADVA L+W+LHDW D+ECIKILK CKEAIP+  GKVIIIE ++    
Sbjct: 241 EHVAGNMFDFVPHADVAFLKWILHDWEDEECIKILKKCKEAIPKSGGKVIIIEAIIIEAE 300

Query: 301 -DEKEDNKFLDVGLMLDLGMMAHTNKGKERTCEEWAYIINKAGFSNSMFPLKKTRKKKWI 348
             EK   K  DVGLM DL MMAHTNKG+ERT EEWA++I++AGF+       +  +    
Sbjct: 301 KGEKMKKKLSDVGLMFDLVMMAHTNKGRERTAEEWAFLIHQAGFTRHTITPLQAIQSLIQ 358

[Close]

BLAST of Tan0004770 vs. NCBI nr
Match: XP_008447904.1 (PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo])

HSP 1 Score: 465.7 bits (1197), Expect = 3.6e-127
Identity = 239/363 (65.84%), Postives = 281/363 (77.41%), Query Frame = 0


Query: 1   MELSHKESSSRKEEDDHAILQMWKYIYGFTEIAAIKCAIQLKIADSIEGYGSPMTLSQLS 60
           ME+ HKESSS KEED  AI+QMW+YI+ FTE+AAIKCAI LKIAD IE YGSP+TLSQLS
Sbjct: 1   MEVKHKESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCAIDLKIADIIESYGSPVTLSQLS 60

Query: 61  STLNCSSSLLYRILRFLIHRQIFKEETIDETENLRGYAQTPLSRLLTTNSENSMIPLFLL 120
           STLNCSSSLLYRILRFLIHR IFK ETID  EN  GY+QTP+SRLL  N ENSM PL LL
Sbjct: 61  STLNCSSSLLYRILRFLIHRGIFKRETID--ENQIGYSQTPMSRLLAINVENSMAPLLLL 120

Query: 121 ESSPVMLAAWLNLSVHVKNSETVPFEVAHGMDLWSYVAENSKHNILFNEAMACSARVITC 180
           E+SPVMLA W +LS H+KNS+T PFE+AHG DLW+Y   N +HN+LFNEAMACSA+VI  
Sbjct: 121 ETSPVMLAPWQHLSAHLKNSDTSPFEIAHGKDLWNYAEANHEHNLLFNEAMACSAKVIVS 180

Query: 181 AILELGCGGDMFNGVRTLVDVGGGNGTTLSIFLKACPWIQGGINFDLPHV---------- 240
           AI+E GC GD+F+GV  LVDVGGGNG+TL+I +K CPW++ GINFDLPHV          
Sbjct: 181 AIIE-GC-GDVFDGVGCLVDVGGGNGSTLNILVKVCPWMK-GINFDLPHVVCASPQYENV 240

Query: 241 -HVAGDMFNFVPKADVALLQWVLHDWNDDECIKILKNCKEAIPEDIGKVIIIEIVM---- 300
            HVAG+MF+FVP ADVA L+W+LHDW D+ECIKILK CKEAIP+  GKVIIIE ++    
Sbjct: 241 EHVAGNMFDFVPHADVAFLKWILHDWEDEECIKILKKCKEAIPKSGGKVIIIEAIIIEAE 300

Query: 301 -DEKEDNKFLDVGLMLDLGMMAHTNKGKERTCEEWAYIINKAGFSNSMFPLKKTRKKKWI 348
             EK   K  DVGLM DL MMAHTNKG+ERT EEWA++I++AGF+       +  +    
Sbjct: 301 KGEKMKKKLSDVGLMFDLVMMAHTNKGRERTAEEWAFLIHQAGFTRHTITPLQAIQSLIQ 358

[Close]

BLAST of Tan0004770 vs. ExPASy TrEMBL
Match: A0A5A7TGF8 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold142G004180 PE=3 SV=1)

HSP 1 Score: 468.0 bits (1203), Expect = 3.5e-128
Identity = 240/363 (66.12%), Postives = 282/363 (77.69%), Query Frame = 0


Query: 1   MELSHKESSSRKEEDDHAILQMWKYIYGFTEIAAIKCAIQLKIADSIEGYGSPMTLSQLS 60
           ME+ HKESSS KEED  AI+QMW+YI+ FTE+AAIKCAI LKIAD IE YGSP+TLSQLS
Sbjct: 1   MEVKHKESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCAIDLKIADIIESYGSPVTLSQLS 60

Query: 61  STLNCSSSLLYRILRFLIHRQIFKEETIDETENLRGYAQTPLSRLLTTNSENSMIPLFLL 120
           STLNCSSSLLYRILRFLIHR IFK ETID  EN  GY+QTP+SRLL TN ENSM PL LL
Sbjct: 61  STLNCSSSLLYRILRFLIHRGIFKRETID--ENQIGYSQTPMSRLLATNVENSMAPLLLL 120

Query: 121 ESSPVMLAAWLNLSVHVKNSETVPFEVAHGMDLWSYVAENSKHNILFNEAMACSARVITC 180
           E+SPVMLA W +LS H+KNS+T PFE+AHG DLW+Y   N +HN+LFNEAMACSA+VI  
Sbjct: 121 ETSPVMLAPWQHLSAHLKNSDTSPFEIAHGKDLWNYAEANHEHNLLFNEAMACSAKVIVS 180

Query: 181 AILELGCGGDMFNGVRTLVDVGGGNGTTLSIFLKACPWIQGGINFDLPHV---------- 240
           AI+E GC GD+F+GV  LVDVGGGNG+TL+I +K CPW++ GINFDLPHV          
Sbjct: 181 AIIE-GC-GDVFDGVGCLVDVGGGNGSTLNILVKVCPWMK-GINFDLPHVVCASPQYENV 240

Query: 241 -HVAGDMFNFVPKADVALLQWVLHDWNDDECIKILKNCKEAIPEDIGKVIIIEIVM---- 300
            HVAG+MF+FVP ADVA L+W+LHDW D+ECIKILK CKEAIP+  GKVIIIE ++    
Sbjct: 241 EHVAGNMFDFVPHADVAFLKWILHDWEDEECIKILKKCKEAIPKSGGKVIIIEAIIIEAE 300

Query: 301 -DEKEDNKFLDVGLMLDLGMMAHTNKGKERTCEEWAYIINKAGFSNSMFPLKKTRKKKWI 348
             EK   K  DVGLM DL MMAHTNKG+ERT EEWA++I++AGF+       +  +    
Sbjct: 301 KGEKMKKKLSDVGLMFDLVMMAHTNKGRERTAEEWAFLIHQAGFTRHTITPLQAIQSLIQ 358

[Close]

BLAST of Tan0004770 vs. ExPASy TrEMBL
Match: A0A1S3BJE6 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC103490250 PE=3 SV=1)

HSP 1 Score: 465.7 bits (1197), Expect = 1.7e-127
Identity = 239/363 (65.84%), Postives = 281/363 (77.41%), Query Frame = 0


Query: 1   MELSHKESSSRKEEDDHAILQMWKYIYGFTEIAAIKCAIQLKIADSIEGYGSPMTLSQLS 60
           ME+ HKESSS KEED  AI+QMW+YI+ FTE+AAIKCAI LKIAD IE YGSP+TLSQLS
Sbjct: 1   MEVKHKESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCAIDLKIADIIESYGSPVTLSQLS 60

Query: 61  STLNCSSSLLYRILRFLIHRQIFKEETIDETENLRGYAQTPLSRLLTTNSENSMIPLFLL 120
           STLNCSSSLLYRILRFLIHR IFK ETID  EN  GY+QTP+SRLL  N ENSM PL LL
Sbjct: 61  STLNCSSSLLYRILRFLIHRGIFKRETID--ENQIGYSQTPMSRLLAINVENSMAPLLLL 120

Query: 121 ESSPVMLAAWLNLSVHVKNSETVPFEVAHGMDLWSYVAENSKHNILFNEAMACSARVITC 180
           E+SPVMLA W +LS H+KNS+T PFE+AHG DLW+Y   N +HN+LFNEAMACSA+VI  
Sbjct: 121 ETSPVMLAPWQHLSAHLKNSDTSPFEIAHGKDLWNYAEANHEHNLLFNEAMACSAKVIVS 180

Query: 181 AILELGCGGDMFNGVRTLVDVGGGNGTTLSIFLKACPWIQGGINFDLPHV---------- 240
           AI+E GC GD+F+GV  LVDVGGGNG+TL+I +K CPW++ GINFDLPHV          
Sbjct: 181 AIIE-GC-GDVFDGVGCLVDVGGGNGSTLNILVKVCPWMK-GINFDLPHVVCASPQYENV 240

Query: 241 -HVAGDMFNFVPKADVALLQWVLHDWNDDECIKILKNCKEAIPEDIGKVIIIEIVM---- 300
            HVAG+MF+FVP ADVA L+W+LHDW D+ECIKILK CKEAIP+  GKVIIIE ++    
Sbjct: 241 EHVAGNMFDFVPHADVAFLKWILHDWEDEECIKILKKCKEAIPKSGGKVIIIEAIIIEAE 300

Query: 301 -DEKEDNKFLDVGLMLDLGMMAHTNKGKERTCEEWAYIINKAGFSNSMFPLKKTRKKKWI 348
             EK   K  DVGLM DL MMAHTNKG+ERT EEWA++I++AGF+       +  +    
Sbjct: 301 KGEKMKKKLSDVGLMFDLVMMAHTNKGRERTAEEWAFLIHQAGFTRHTITPLQAIQSLIQ 358

[Close]

BLAST of Tan0004770 vs. ExPASy TrEMBL
Match: A0A0A0K629 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G018730 PE=3 SV=1)

HSP 1 Score: 464.2 bits (1193), Expect = 5.0e-127
Identity = 235/362 (64.92%), Postives = 286/362 (79.01%), Query Frame = 0


Query: 1   MELSHKESSSRKEEDDHAILQMWKYIYGFTEIAAIKCAIQLKIADSIEGYGSPMTLSQLS 60
           ME+ HKESSS KEED  AI+QMW+YI+ FTE+AAIKC + LKIAD IE YGSP+TLSQLS
Sbjct: 1   MEVKHKESSSEKEEDGQAIIQMWRYIFRFTEMAAIKCVVDLKIADIIESYGSPVTLSQLS 60

Query: 61  STLNCSSSLLYRILRFLIHRQIFKEETIDETENLRGYAQTPLSRLLTTNSENSMIPLFLL 120
           STLNCSSSLL+RILRFLIHR IFK+ETID  EN  GY+QTP+SRLL TN ENSM PL LL
Sbjct: 61  STLNCSSSLLHRILRFLIHRGIFKQETID--ENQIGYSQTPMSRLLATNVENSMAPLLLL 120

Query: 121 ESSPVMLAAWLNLSVHVKNSETVPFEVAHGMDLWSYVAENSKHNILFNEAMACSARVITC 180
           E+SPVMLA W +LS H+KNS+T+PFE+AHG DLW Y   N +HN+LFNEAMAC A++I  
Sbjct: 121 ETSPVMLAPWQHLSGHLKNSDTLPFEIAHGKDLWRYAEANHEHNLLFNEAMACGAKMIVS 180

Query: 181 AILELGCGGDMFNGVRTLVDVGGGNGTTLSIFLKACPWIQGGINFDLPHV---------- 240
           AI+E GC GD+F+GVR LVDVGGGNG+TLSI +KA PW++ GINFDLPHV          
Sbjct: 181 AIIE-GC-GDVFDGVRCLVDVGGGNGSTLSILVKAYPWMK-GINFDLPHVVCASPQYENV 240

Query: 241 -HVAGDMFNFVPKADVALLQWVLHDWNDDECIKILKNCKEAIPEDIGKVIIIEIVMD--- 300
            HVAG+MF+FVP ADVA L+W+LHDW+D+ECIKILK CKE+IP+  GKVIIIE +++   
Sbjct: 241 EHVAGNMFDFVPNADVAFLKWILHDWDDEECIKILKKCKESIPKSGGKVIIIEAIIEAEK 300

Query: 301 -EKEDNKFLDVGLMLDLGMMAHTNKGKERTCEEWAYIINKAGFSNSMFPLKKTRKKKWIC 348
            EK++ K  DVGLM DL MMAHTN+GKERT +EWA++I++AGF+       +  +    C
Sbjct: 301 GEKKNKKLSDVGLMFDLVMMAHTNRGKERTAQEWAFLIHQAGFTTHTITPIQAIQSLIQC 357

[Close]

BLAST of Tan0004770 vs. ExPASy TrEMBL
Match: A0A6J1C5N1 ((R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LOC111007607 PE=3 SV=1)

HSP 1 Score: 449.1 bits (1154), Expect = 1.7e-122
Identity = 225/349 (64.47%), Postives = 274/349 (78.51%), Query Frame = 0


Query: 1   MELSHKESSSRKEED-DHAILQMWKYIYGFTEIAAIKCAIQLKIADSIEGYGSPMTLSQL 60
           M+L+ KES+S+KEED +HA +QMW YI+GFTE+A IKCAI+LKIAD+IE +GSPMTLS+L
Sbjct: 11  MDLALKESTSKKEEDHEHARVQMWNYIFGFTEVAIIKCAIELKIADTIESHGSPMTLSKL 70

Query: 61  SSTLNCSSSLLYRILRFLIHRQIFKEETIDETENLRGYAQTPLSRLLTTNSENSMIPLFL 120
           SS LNCS  LLYRILRFL HR IFKEET    +NL GYAQTPLSRLL +NS NSM P  L
Sbjct: 71  SSALNCSPPLLYRILRFLTHRGIFKEET--TAQNLTGYAQTPLSRLLASNSNNSMAPFLL 130

Query: 121 LESSPVMLAAWLNLSVHVKNSETVPFEVAHGMDLWSYVAENSKHNILFNEAMACSARVIT 180
           LES+PVMLA W NLS  +K  +  PFE  HG DLWSY A +  HN +FN+AMAC+ARVIT
Sbjct: 131 LESNPVMLAPWHNLSALIKAKDGTPFEATHGKDLWSYTAADPTHNKIFNDAMACAARVIT 190

Query: 181 CAILELGCGGDMFNGVRTLVDVGGGNGTTLSIFLKACPWIQGGINFDLPHV--------- 240
              +  GC G++F GV +LVDVGGGNGT+LS+ +KACPWIQ GINFDLPHV         
Sbjct: 191 VPAILEGC-GEIFEGVGSLVDVGGGNGTSLSMIVKACPWIQ-GINFDLPHVVSVAQECVG 250

Query: 241 --HVAGDMFNFVPKADVALLQWVLHDWNDDECIKILKNCKEAIPEDIGKVIIIEIVMDEK 300
             HV G+MF+ +PKAD A + WVLHDWND++CIKILKNC++AIP+ IGKVII+E V+D  
Sbjct: 251 VQHVEGNMFDCIPKADAAFIMWVLHDWNDEDCIKILKNCRDAIPKKIGKVIIVEAVIDGN 310

Query: 301 EDNKFLDVGLMLDLGMMAHTNKGKERTCEEWAYIINKAGFSN-SMFPLK 337
           E+N   DVGL+LD+ MMAHTNKGKERT EEWAY+++KAGF+  ++ P+K
Sbjct: 311 EENNLWDVGLLLDMVMMAHTNKGKERTSEEWAYVLHKAGFTRYTITPVK 355

[Close]

BLAST of Tan0004770 vs. ExPASy TrEMBL
Match: A0A6J1GNG0 ((RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=LOC111455537 PE=3 SV=1)

HSP 1 Score: 439.9 bits (1130), Expect = 1.0e-119
Identity = 232/354 (65.54%), Postives = 274/354 (77.40%), Query Frame = 0


Query: 1   MELSHKESSSRKEEDDHAILQMWKYIYGFTEIAAIKCAIQLKIADSIEGYGSPMTLSQLS 60
           ME+SHK+ SS+KEED  AI QMWKY +GFTE+AAIKC I LKIAD IEG GSPMTL++LS
Sbjct: 1   MEISHKKCSSQKEEDGQAITQMWKYTFGFTELAAIKCIIDLKIADIIEGNGSPMTLAELS 60

Query: 61  STLNCSSSLLYRILRFLIHRQIFKEETIDETENLRGYAQTPLSRLLTTNSENSMIPLFLL 120
           +T NCSS+LLYRI+RFLIHR IF+EET  E+    GYA TPLSRLL TN ENSM P+ L 
Sbjct: 61  TTSNCSSTLLYRIMRFLIHRGIFREETTAESH--IGYAHTPLSRLLATNVENSMAPMILF 120

Query: 121 ESSPVMLAAWLNLSVHVKNSETVPFEVAHGMDLWSYVAENSKHNILFNEAMACSARVITC 180
           + SPVMLA W  LS H+KNS+T PFE+AHG D+WSY A N ++N+L NEAMACSARV T 
Sbjct: 121 KISPVMLAPWQCLSAHLKNSDTSPFEIAHGKDIWSYAAANPEYNMLCNEAMACSARVTTS 180

Query: 181 AILELGCGGDMFNGVRTLVDVGGGNGTTLSIFLKACPWIQGGINFDLPHV---------- 240
           AILE GC GD+F+GV  LVDVGGGNG+TLSI +KACPW++ GINFDLPHV          
Sbjct: 181 AILE-GC-GDIFDGVGCLVDVGGGNGSTLSILVKACPWMR-GINFDLPHVVSASLEYDNV 240

Query: 241 -HVAGDMFNFVPKADVALLQWVLHDWNDDECIKILKNCKEAIPEDIGKVIIIEIVMD--E 300
            HVAGDMF+F+PKADVA L+ +LHDW+D+ECIKILKNCKEAI ++ GKVIIIE V++  E
Sbjct: 241 QHVAGDMFDFIPKADVAFLKCILHDWDDEECIKILKNCKEAISKNKGKVIIIEAVIEGTE 300

Query: 301 KEDNKFL------------DVGLMLDLGMMAHTNKGKERTCEEWAYIINKAGFS 330
           K D   +            DVGLM DL MMA+TNKGKERT EEWAY+I+ AG+S
Sbjct: 301 KSDVGLMFDLLMIEGTEKSDVGLMFDLLMMAYTNKGKERTHEEWAYLIHAAGYS 349

[Close]

BLAST of Tan0004770 vs. TAIR 10
Match: AT4G35160.1 (O-methyltransferase family protein )

HSP 1 Score: 295.0 bits (754), Expect = 7.8e-80
Identity = 164/359 (45.68%), Postives = 226/359 (62.95%), Query Frame = 0


Query: 1   MELSHKESSSRK--EEDDHAILQMWKYIYGFTEIAAIKCAIQLKIADSIEGYGS--PMTL 60
           ++ +  E + RK  +E+  A L +WKY++GF +IAA KCAI LKI ++IE + S  P+TL
Sbjct: 10  LDRNKMEDNKRKVLDEEAKASLDIWKYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTL 69

Query: 61  SQLSSTLNCSSSLLYRILRFLIHRQIFKEETIDETENL-RGYAQTPLSR--LLTTNSENS 120
           ++LSS ++ S S L RI+RFL+H+ IFKE  I   + L  GY  TPLSR  ++T     S
Sbjct: 70  AELSSAVSASPSHLRRIMRFLVHQGIFKE--IPTKDGLATGYVNTPLSRRLMITRRDGKS 129

Query: 121 MIPLFLLESSPVMLAAWLNL----SVHVKNSETVPFEVAHGMDLWSYVAENSKHNILFNE 180
           + P  L E++P MLA WL L    S  V  S   PF+  HG D+WS+  +N   + + NE
Sbjct: 130 LAPFVLFETTPEMLAPWLRLSSVVSSPVNGSTPPPFDAVHGKDVWSFAQDNPFLSDMINE 189

Query: 181 AMACSARVITCAILELGCGGDMFNGVRTLVDVGGGNGTTLSIFLKACPWIQGGINFDLPH 240
           AMAC AR +   +   G    +F+GV T+VDVGGG G T+ + +K  PWI+ G NFDLPH
Sbjct: 190 AMACDARRVVPRV--AGACHGLFDGVTTMVDVGGGTGETMGMLVKEFPWIK-GFNFDLPH 249

Query: 241 V-----------HVAGDMFNFVPKADVALLQWVLHDWNDDECIKILKNCKEAIPEDIGKV 300
           V           +V GDMF+ +P  D   ++WVLHDW D +CIKILKNCKEA+P +IGKV
Sbjct: 250 VIEVAEVLDGVENVEGDMFDSIPACDAIFIKWVLHDWGDKDCIKILKNCKEAVPPNIGKV 309

Query: 301 IIIEIVMDE--------KEDNKFLDVGLMLDLGMMAHTNKGKERTCEEWAYIINKAGFS 330
           +I+E V+ E        + D K   V LMLD+ MMAHT+ GKERT +EW +++ +AGF+
Sbjct: 310 LIVESVIGENKKTMIVDERDEKLEHVRLMLDMVMMAHTSTGKERTLKEWDFVLKEAGFA 363

[Close]

BLAST of Tan0004770 vs. TAIR 10
Match: AT4G35150.1 (O-methyltransferase family protein )

HSP 1 Score: 255.8 bits (652), Expect = 5.2e-68
Identity = 146/347 (42.07%), Postives = 202/347 (58.21%), Query Frame = 0


Query: 7   ESSSRK--EEDDHAILQMWKYIYGFTEIAAIKCAIQLKIADSIEGYGS--PMTLSQLSST 66
           E S R   +E+  A L +W+Y++GF +IAA KCAI LKI ++IE + S  P+TLS+LSS 
Sbjct: 2   EESKRNLLDEEAKASLDIWRYVFGFADIAAAKCAIDLKIPEAIENHPSSQPVTLSELSSA 61

Query: 67  LNCSSSLLYRILRFLIHRQIFKEETIDETENL-RGYAQTPLSRLLTTNSENSMIPLFLLE 126
           ++ S S L RI+RFL+H+ +FKE  +   + L  GY  TPLSR +               
Sbjct: 62  VSASPSHLRRIMRFLVHQGLFKE--VPTKDGLATGYTNTPLSRRMMITK----------- 121

Query: 127 SSPVMLAAWLNLSVHVKNSETVPFEVAHGMDLWSYVAENSKHNILFNEAMACSARVITCA 186
                                      HG DLW++  +N  H+ L NEAMAC AR +   
Sbjct: 122 --------------------------LHGKDLWAFAQDNLCHSQLINEAMACDARRVVPR 181

Query: 187 ILELGCGGDMFNGVRTLVDVGGGNGTTLSIFLKACPWIQGGINFDLPHV----------- 246
           +   G    +F+GV T+VDVGGG G T+ I +K  PWI+ G NFDLPHV           
Sbjct: 182 V--AGACQGLFDGVATVVDVGGGTGETMGILVKEFPWIK-GFNFDLPHVIEVAQVLDGVE 241

Query: 247 HVAGDMFNFVPKADVALLQWVLHDWNDDECIKILKNCKEAIPEDIGKVIIIEIVMDEKE- 306
           +V GDMF+ +P +D  +++WVLHDW D +CIKILKNCKEA+  +IGKV+I+E V+ EK+ 
Sbjct: 242 NVEGDMFDSIPASDAVIIKWVLHDWGDKDCIKILKNCKEAVLPNIGKVLIVECVIGEKKN 301

Query: 307 -------DNKFLDVGLMLDLGMMAHTNKGKERTCEEWAYIINKAGFS 330
                  D+K   V L LD+ MM HT+ GKERT +EW +++ +AGF+
Sbjct: 302 TMIAEERDDKLEHVRLQLDMVMMVHTSTGKERTLKEWDFVLTEAGFA 306

[Close]

BLAST of Tan0004770 vs. TAIR 10
Match: AT5G54160.1 (O-methyltransferase 1 )

HSP 1 Score: 171.0 bits (432), Expect = 1.7e-42
Identity = 113/335 (33.73%), Postives = 178/335 (53.13%), Query Frame = 0


Query: 15  DDHAILQMWKYIYGFTEIAAIKCAIQLKIADSIEGYGSPMTLSQLSSTLNCSSS----LL 74
           DD A L   +         A+K A++L + + +   GSPM+ ++++S L   +     +L
Sbjct: 16  DDEAALFAMQLASASVLPMALKSALELDLLEIMAKNGSPMSPTEIASKLPTKNPEAPVML 75

Query: 75  YRILRFLIHRQIF----KEETIDETENLRGYAQTPLSRLLTTNSEN-SMIPLFLLESSPV 134
            RILR L    +     ++ + D  E +  Y   P+ + LT N +  S+  L L+    V
Sbjct: 76  DRILRLLTSYSVLTCSNRKLSGDGVERI--YGLGPVCKYLTKNEDGVSIAALCLMNQDKV 135

Query: 135 MLAAWLNLSVHVKNSETVPFEVAHGMDLWSYVAENSKHNILFNEAMACSARVITCAILEL 194
           ++ +W +L   + +   +PF  A+GM  + Y   + + N +FN  M+  + +    ILE 
Sbjct: 136 LMESWYHLKDAILDG-GIPFNKAYGMSAFEYHGTDPRFNKVFNNGMSNHSTITMKKILET 195

Query: 195 GCGGDMFNGVRTLVDVGGGNGTTLSIFLKACPWIQGGINFDLPHV-----------HVAG 254
             G   F G+ +LVDVGGG G TL + +   P ++ GINFDLPHV           HV G
Sbjct: 196 YKG---FEGLTSLVDVGGGIGATLKMIVSKYPNLK-GINFDLPHVIEDAPSHPGIEHVGG 255

Query: 255 DMFNFVPKADVALLQWVLHDWNDDECIKILKNCKEAIPEDIGKVIIIEIVMDEKEDNKFL 314
           DMF  VPK D   ++W+ HDW+D+ C+K LKNC E++PED GKVI+ E ++ E  D+   
Sbjct: 256 DMFVSVPKGDAIFMKWICHDWSDEHCVKFLKNCYESLPED-GKVILAECILPETPDSSLS 315

Query: 315 DVGLM-LDLGMMAHTNKGKERTCEEWAYIINKAGF 329
              ++ +D  M+AH   GKERT +E+  +   +GF
Sbjct: 316 TKQVVHVDCIMLAHNPGGKERTEKEFEALAKASGF 342

[Close]

BLAST of Tan0004770 vs. TAIR 10
Match: AT1G77520.1 (O-methyltransferase family protein )

HSP 1 Score: 164.1 bits (414), Expect = 2.1e-40
Identity = 111/323 (34.37%), Postives = 168/323 (52.01%), Query Frame = 0


Query: 35  IKCAIQLKIADSIEGYGSPMTLS--QLSSTLNCSSS------LLYRILRFLIHRQIFKEE 94
           +K A +L + D+I   G+   LS  +++ +L    +      LL R+L  L+   I K  
Sbjct: 46  LKAAFELGVIDTIAAAGNDTWLSPCEIACSLPTKPTNPEAPVLLDRMLSLLVSHSILKCR 105

Query: 95  TIDETEN------LRGYAQTPLSRLLTTNSE--NSMIPLFLLESSPVMLAAWLNLSVHVK 154
            I+  EN       R YA  P+ +    +S+   S++PLF+L  + V    W NL   + 
Sbjct: 106 MIETGENGRTGKIERVYAAEPVCKYFLRDSDGTGSLVPLFMLLHTQVFFKTWTNLKDVIL 165

Query: 155 NSETVPFEVAHGMDLWSYVAENSKHNILFNEAMACSARVITCAILELGCGGDMFNGVRTL 214
                 F  AHGM ++ Y+  +     LFN AM+  + +I   +L++  G   F  V TL
Sbjct: 166 EGRDA-FNSAHGMKIFEYINSDQPFAELFNRAMSEPSTMIMKKVLDVYRG---FEDVNTL 225

Query: 215 VDVGGGNGTTLSIFLKACPWIQGGINFDLPHV-----------HVAGDMFNFVPKADVAL 274
           VDVGGGNGT L +     P I+ G+NFDL  V           HV+GDMF  VPK D   
Sbjct: 226 VDVGGGNGTVLGLVTSKYPHIK-GVNFDLAQVLTQAPFYPGVEHVSGDMFVEVPKGDAVF 285

Query: 275 LQWVLHDWNDDECIKILKNCKEAIPEDIGKVIIIEIVMDEKEDNKFLDVGLM--LDLGMM 329
           ++W+LHDW D++CIKILKNC +++PE  GK+II+E V  ++     L    +  +DL M+
Sbjct: 286 MKWILHDWGDEDCIKILKNCWKSLPEK-GKIIIVEFVTPKEPKGGDLSSNTVFAMDLLML 345

[Close]

BLAST of Tan0004770 vs. TAIR 10
Match: AT5G53810.1 (O-methyltransferase family protein )

HSP 1 Score: 164.1 bits (414), Expect = 2.1e-40
Identity = 110/325 (33.85%), Postives = 171/325 (52.62%), Query Frame = 0


Query: 35  IKCAIQLKIADSIEGYG------SPMTLSQLSSTLNC---SSSLLYRILRFLIHRQIFKE 94
           +K A++L + D+I   G      SP  ++    T  C   + +LL R+LRFL+   + K 
Sbjct: 42  LKAALELGVIDTITTVGGGDLWLSPSEIALRLPTKPCNLEAPALLDRMLRFLVSHSVLKC 101

Query: 95  ETIDETENLRG-----YAQTPLSRLLTTNSEN---SMIPLFLLESSPVMLAAWLNLSVHV 154
            T+ E     G     YA  P+ + L   S++   S   LF+L+ S V +  W +L   +
Sbjct: 102 RTVIEENGQTGKVERVYAAEPVCKYLLNKSDDVSGSFASLFMLDLSDVFIKTWTHLEDVI 161

Query: 155 KNSETVPFEVAHGMDLWSYVAENSKHNILFNEAMACSARVITCAILELGCGGDMFNGVRT 214
                  F  AHGM L+ Y+  + +   +FN AM  S+ ++T  +L+   G   F  V+T
Sbjct: 162 LEGRDA-FSSAHGMKLFEYIQADERFGKVFNRAMLESSTMVTEKVLKFYEG---FKDVKT 221

Query: 215 LVDVGGGNGTTLSIFLKACPWIQGGINFDLPHV-----------HVAGDMFNFVPKADVA 274
           LVDVGGG G TL +     P +  GINFDL  V           HVAGDMF  +PK D  
Sbjct: 222 LVDVGGGLGNTLGLITSKYPHLI-GINFDLAPVLANAHSYPGVNHVAGDMFIKIPKGDAI 281

Query: 275 LLQWVLHDWNDDECIKILKNCKEAIPEDIGKVIIIEIV--MDEKEDNKFLDVGLMLDLGM 330
            ++W+LHDW D++C+ ILKNC +++ E+ GK+II+E+V  ++ K  +   ++   +D+ M
Sbjct: 282 FMKWILHDWTDEQCVAILKNCWKSLEEN-GKLIIVEMVTPVEAKSGDICSNIVFGMDMTM 341

The following BLAST results are available for this feature:

BLAST of Tan0004770 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9T003	1.1e-78	45.68	Acetylserotonin O-methyltransferase OS=Arabidopsis thaliana OX=3702 GN=ASMT PE=1...	[more]
Q7XB10	4.2e-62	37.25	3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase 2 OS=Papaver somniferu...	[more]
Q9LEL6	9.3e-62	41.09	(RS)-norcoclaurine 6-O-methyltransferase OS=Coptis japonica OX=3442 PE=1 SV=1	[more]
Q5C9L7	3.5e-61	40.12	(RS)-norcoclaurine 6-O-methyltransferase OS=Thalictrum flavum subsp. glaucum OX=...	[more]
Q9LEL5	6.0e-61	39.58	3'-hydroxy-N-methyl-(S)-coclaurine 4'-O-methyltransferase OS=Coptis japonica OX=...	[more]

BLAST of Tan0004770 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038888809.1	3.1e-131	70.38	acetylserotonin O-methyltransferase-like [Benincasa hispida]	[more]
XP_038887538.1	6.5e-129	70.38	acetylserotonin O-methyltransferase-like isoform X1 [Benincasa hispida]	[more]
XP_038887354.1	1.1e-128	70.09	acetylserotonin O-methyltransferase-like isoform X1 [Benincasa hispida]	[more]
KAA0040349.1	7.2e-128	66.12	(RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo var. makuwa] >TYK233...	[more]
XP_008447904.1	3.6e-127	65.84	PREDICTED: (RS)-norcoclaurine 6-O-methyltransferase-like [Cucumis melo]	[more]

BLAST of Tan0004770 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7TGF8	3.5e-128	66.12	(RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo var. makuwa OX=119...	[more]
A0A1S3BJE6	1.7e-127	65.84	(RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucumis melo OX=3656 GN=LOC1034...	[more]
A0A0A0K629	5.0e-127	64.92	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_7G018730 PE=3 SV=1	[more]
A0A6J1C5N1	1.7e-122	64.47	(R,S)-reticuline 7-O-methyltransferase-like OS=Momordica charantia OX=3673 GN=LO...	[more]
A0A6J1GNG0	1.0e-119	65.54	(RS)-norcoclaurine 6-O-methyltransferase-like OS=Cucurbita moschata OX=3662 GN=L...	[more]

BLAST of Tan0004770 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35160.1	7.8e-80	45.68	O-methyltransferase family protein	[more]
AT4G35150.1	5.2e-68	42.07	O-methyltransferase family protein	[more]
AT5G54160.1	1.7e-42	33.73	O-methyltransferase 1	[more]
AT1G77520.1	2.1e-40	34.37	O-methyltransferase family protein	[more]
AT5G53810.1	2.1e-40	33.85	O-methyltransferase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016461	O-methyltransferase COMT-type	PIRSF	PIRSF005739	O-mtase	coord: 1..339 e-value: 2.1E-102 score: 340.6
IPR016461	O-methyltransferase COMT-type	PANTHER	PTHR11746	O-METHYLTRANSFERASE	coord: 11..331
IPR016461	O-methyltransferase COMT-type	PROSITE	PS51683	SAM_OMT_II	coord: 23..347 score: 59.74403
IPR001077	O-methyltransferase domain	PFAM	PF00891	Methyltransf_2	coord: 130..329 e-value: 7.7E-42 score: 143.0
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 4..106 e-value: 1.6E-24 score: 87.5
IPR012967	Plant methyltransferase dimerisation	PFAM	PF08100	Dimerisation	coord: 34..77 e-value: 3.6E-8 score: 33.3
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 107..339 e-value: 1.6E-62 score: 212.7
None	No IPR available	PIRSR	PIRSR005739-1	PIRSR005739-1	coord: 33..331 e-value: 1.9E-73 score: 244.9
None	No IPR available	PANTHER	PTHR11746:SF191	3'-HYDROXY-N-METHYL-(S)-COCLAURINE 4'-O-METHYLTRANSFERASE 2-LIKE	coord: 11..331
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 12..110
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 112..330

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0004770.1	Tan0004770.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019438	aromatic compound biosynthetic process
biological_process	GO:0032259	methylation
molecular_function	GO:0008171	O-methyltransferase activity
molecular_function	GO:0046983	protein dimerization activity
molecular_function	GO:0008757	S-adenosylmethionine-dependent methyltransferase activity
molecular_function	GO:0008168	methyltransferase activity