Tan0004539 (gene) Snake gourd v1

BLAST of Tan0004539 vs. ExPASy Swiss-Prot
Match: P0C897 (Putative UPF0481 protein At3g02645 OS=Arabidopsis thaliana OX=3702 GN=At3g02645 PE=3 SV=1)

HSP 1 Score: 156.4 bits (394), Expect = 9.2e-37
Identity = 147/560 (26.25%), Postives = 267/560 (47.68%), Query Frame = 0

Query: 6   PIVSAT----FNQSQWVIQMNELLHRD-DLSAASQSLVSVYKLPDSVRDVDPAAYLPRRV 65
           PI S+T    F++++WVI + + L  + +     +  VS++ +P ++    P +Y P RV
Sbjct: 6   PIFSSTEQHRFDETRWVINVQKSLDAELEEHDLEEVTVSIFNVPKALMCSHPDSYTPHRV 65

Query: 66  AFGPYHHFRPDLSKMDLFKL---EKAKNLKWGPQIHELADQLKPLELRIRACFDQSLEIN 125
           + GPYH  +P+L +M+ +KL    K +N     + H+L ++L+ +E++IRAC+ + +  N
Sbjct: 66  SIGPYHCLKPELHEMERYKLMIARKIRNQYNSFRFHDLVEKLQSMEIKIRACYHKYIGFN 125

Query: 126 GETLSWVVLIDGLYLLELLQRRYYSRPLKLPLYIFYGKLWSESEIISDMLKLENQIPLLV 185
           GETL W++ +D  +L+E L+   + +   L   +        +EI+ D++ +ENQIPL V
Sbjct: 126 GETLLWIMAVDSSFLIEFLKIYSFRKVETLINRV------GHNEILRDIMMIENQIPLFV 185

Query: 186 LKEIL-----SKDEENDLTSYLYKFSTSVSSFELPHLLTREEILVFYSNIPEINDLSHHL 245
           L++ L     S +  +DL   L    T +     P ++  ++  +  +   E N    H+
Sbjct: 186 LRKTLEFQLESTESADDL---LLSVLTGLCKDLSPLVIKFDDDQILKAQFQECN----HI 245

Query: 246 LHFLYFSVLLIPYDKYEAMLKECTNSASSPRYLQHFGVFSSHIAFIQQIKQTYGIV---- 305
           L FLY   +++P  + E    E        R  ++ G  +  I F+ +IK  +  V    
Sbjct: 246 LDFLY--QMIVPRIEEE----ELEEDDEENRADENGG--NRAIRFMDEIKHQFKRVFASR 305

Query: 306 ----------QRLLRLLGYVAKLKLNEKQII-----------------------PL---- 365
                     + +  L G++A LKL+   +                        PL    
Sbjct: 306 PADLILRFPWRIISNLPGFMA-LKLSADYLFTRQENEATTTRQESVSILDIEKPPLVEEL 365

Query: 366 -IPSASDLKKSGFTFKSTKNG-ILKCSFDETTLTLTLSSIHLDGFSQVLLRNLVAFEALA 425
            IPS SDL K+G  FK T +G I   +FD  +    L  I+LD  ++ +LRNLVA+EA  
Sbjct: 366 TIPSVSDLHKAGVRFKPTAHGNISTVTFDSNSGQFYLPVINLDINTETVLRNLVAYEATN 425

Query: 426 ELNPPCLIQYIALMNGLLTNSNDLEILKKAGIVHNHLNSEEEAVELFHGVNNSISHFKEA 485
              P    +Y  L+NG++ +  D+ +L++ G++ + L S++EA E+++G++ S+      
Sbjct: 426 TSGPLVFTRYTELINGIIDSEEDVRLLREQGVLVSRLKSDQEAAEMWNGMSKSVR----- 485

Query: 486 SMLKIHLQTGLDMEITSKFLQEINMYYDMGLKRMVEEINKSYKRCWRSKMKKFVSI---- 506
                              L ++       L + +E++N+ Y   W+ K+ + V +    
Sbjct: 486 -------------------LTKVGF-----LDKTIEDVNRYYTGRWKVKIGRLVEVYVYG 514

BLAST of Tan0004539 vs. ExPASy Swiss-Prot
Match: Q9SD53 (UPF0481 protein At3g47200 OS=Arabidopsis thaliana OX=3702 GN=At3g47200 PE=2 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 9.3e-13
Identity = 105/416 (25.24%), Postives = 173/416 (41.59%), Query Frame = 0

Query: 30  LSAASQSLVSVYKLPDSVRDVDPAAYLPRRVAFGPYHHFRPDLSKMDLFK-------LEK 89
           L +A +    ++++P+S   ++P AY P+ V+ GPYH+    L  +   K       L++
Sbjct: 38  LESAGKESCCIFRVPESFVALNPKAYKPKVVSIGPYHYGEKHLQMIQQHKPRLLQLFLDE 97

Query: 90  AKNLKWGPQIHELADQLKPLELRIRACFDQSLEINGETLSWVVLIDGLYLLELLQRRYYS 149
           AK  K   + + L   +  LE +IR  + + L+  G  L +++++DG ++L +      S
Sbjct: 98  AK--KKDVEENVLVKAVVDLEDKIRKSYSEELK-TGHDLMFMMVLDGCFILMVF--LIMS 157

Query: 150 RPLKLPLYIFYGKLWSESEIISDMLKLENQIPLLVLKEILSKDEENDLTSYLYKFSTSVS 209
             ++L     +   W  S I SD+L LENQ+P  VL+              LY  S    
Sbjct: 158 GNIELSEDPIFSIPWLLSSIQSDLLLLENQVPFFVLQT-------------LYVGSKIGV 217

Query: 210 SFELPHLLTREEILVFYSNIPEINDLSHHLLHFLYFSVLLIPYDKYEAMLKECTNS--AS 269
           S +L  +        F+ N P   + S+   H  Y +  L+   + E  L   + S  AS
Sbjct: 218 SSDLNRIAFH-----FFKN-PIDKEGSYWEKHRNYKAKHLLDLIR-ETFLPNTSESDKAS 277

Query: 270 SPRYLQHFGVFSSHIAFIQQIKQTYGIVQRLLRLLGYVAKLKLNEKQIIPLIPSASDLKK 329
           SP           H+  +Q  +   G V  +             + + +PLI SA  L+ 
Sbjct: 278 SP-----------HVQ-VQLHEGKSGNVPSV-------------DSKAVPLILSAKRLRL 337

Query: 330 SGFTF---KSTKNGILKCSFDETTLTLTLSSIHLDGFSQVLLRNLVAFEALAELNPPCLI 389
            G  F   +S ++ IL     +    L +  +  DGF      N VAFE     +   + 
Sbjct: 338 QGIKFRLRRSKEDSILNVRLKKN--KLQIPQLRFDGFISSFFLNCVAFEQFYTDSSNEIT 397

Query: 390 QYIALMNGLLTNSNDLEILKKAG-IVHNHLNSEEEAVELFHGVNNSISHFKEASML 433
            YI  M  LL N  D+  L+    I+ NH  S  E  E F  ++  +    + S L
Sbjct: 398 TYIVFMGCLLNNEEDVTFLRNDKLIIENHFGSNNEVSEFFKTISKDVVFEVDTSYL 401

BLAST of Tan0004539 vs. NCBI nr
Match: XP_038890580.1 (putative UPF0481 protein At3g02645 [Benincasa hispida])

HSP 1 Score: 631.3 bits (1627), Expect = 7.4e-177
Identity = 328/524 (62.60%), Postives = 411/524 (78.44%), Query Frame = 0

Query: 10  ATFNQSQWVIQMNELLHRDDLSAASQSLVSVYKLPDSVRDVDPAAYLPRRVAFGPYHHFR 69
           A+ ++SQWV Q+N+L+ ++ L  ASQ+ +SV +LP+SV D +PAA+LPR VAFGPYHHFR
Sbjct: 2   ASVDKSQWVHQINDLIDQNALLPASQTPISVVRLPESVHDGNPAAFLPRHVAFGPYHHFR 61

Query: 70  PDLSKMDLFKLEKAKNLKWGPQIHELADQLKPLELRIRACFDQSLEINGETLSWVVLIDG 129
           P+L  M+LFKL KAKNL W PQ+H+LADQL PLEL+IRACFDQ+LEINGETLSWV+LIDG
Sbjct: 62  PELYNMELFKLGKAKNLNWSPQLHKLADQLMPLELKIRACFDQTLEINGETLSWVLLIDG 121

Query: 130 LYLLELLQRRYYSRPLKLPLYIFYGKLWSESEIISDMLKLENQIPLLVLKEILSKDEEND 189
           L+L+ LLQ +Y + PL+ PL I+YGKLWSE EI+SDM+KLENQIPL VL EI  K+  N 
Sbjct: 122 LFLIHLLQNKYNNHPLEFPLGIYYGKLWSEFEIVSDMVKLENQIPLFVLNEICPKEPINF 181

Query: 190 LTSYLYKFSTSVSSFELPHLLTREEILVFYSNIPEINDLSHHLLHFLYFSVLLIPYDKYE 249
           L  ++Y+FS SVS F+LP      E   F SN+PEI +LSHHLLHFLY  +LLIP +   
Sbjct: 182 LAPFMYQFSASVSPFQLPIFNPELECFGFSSNLPEIFNLSHHLLHFLYSLILLIPDEVVV 241

Query: 250 A--MLKECTNSASSPRYLQHFGVFSS--HIAFIQQIKQTYGIVQRLLRLLGYVAKLKLNE 309
           A  M  + +++A++    +   +  S  +IAFIQQ+K+T+G++QRL RLL  + K  L E
Sbjct: 242 AYCMRLDTSSAAAAELLSESLNILGSVINIAFIQQVKETFGLIQRLFRLLSSITKPNLAE 301

Query: 310 KQIIPL--IPSASDLKKSGFTFKSTKNGILKCSFDETTLTLTLSSIHLDGFSQVLLRNLV 369
           K   PL  IPSASDLK +G TFKSTKNGILKC F+E+TLTLTL  IHLDGFSQVLL+NLV
Sbjct: 302 KHHPPLLIIPSASDLKSAGVTFKSTKNGILKCFFNESTLTLTLPCIHLDGFSQVLLKNLV 361

Query: 370 AFEALAELNPPCLIQYIALMNGLLTNSNDLEILKKAGIVHNHLNSEEEAVELFHGVNNSI 429
           AFEA+ ELNPPCL+ Y++LMNGLL NS DL++L+KAGIVHNHLNSEEEAVELF+GV NS 
Sbjct: 362 AFEAMTELNPPCLVNYVSLMNGLLRNSKDLKVLEKAGIVHNHLNSEEEAVELFNGVENST 421

Query: 430 SHFKEASMLKIHLQTGLDMEITSKFLQEINMYYDMGLKRMVEEINKSYKRCWRSKMKKFV 489
              K+ S+LKIHL++GL+ E  SKF+Q++ M Y+MGL  + EEINK Y  CWR KMKKF+
Sbjct: 422 RSLKKESVLKIHLKSGLNFETGSKFIQDVKMSYEMGLGALAEEINKFYGNCWRMKMKKFM 481

Query: 490 SIGFKCFAILFIVFLVVLVIAQFLCHYLSCPGKIFQTSIASHVM 528
           SI FKCFAIL +VFL+VLVIA+F+C++LSCP  IF+TSIA H M
Sbjct: 482 SIVFKCFAILLVVFLIVLVIARFVCNFLSCPLGIFKTSIALHKM 525

BLAST of Tan0004539 vs. NCBI nr
Match: XP_004141645.1 (putative UPF0481 protein At3g02645 [Cucumis sativus] >KGN52693.1 hypothetical protein Csa_008317 [Cucumis sativus])

HSP 1 Score: 622.5 bits (1604), Expect = 3.5e-174
Identity = 328/536 (61.19%), Postives = 407/536 (75.93%), Query Frame = 0

Query: 1   MASNFPIVSATFNQSQWVIQMNELLHRDDLSAASQSLVSVYKLPDSVRDVDPAAYLPRRV 60
           MAS F I++ TF++SQW+ Q+N L+++D L AASQ  +SV++LP+SV DVDP AYLPR V
Sbjct: 1   MASGFSILTGTFDKSQWLHQINNLVNQDALLAASQIPISVFRLPESVHDVDPTAYLPRHV 60

Query: 61  AFGPYHHFRPDLSKMDLFKLEKAKNLKWGPQIHELADQLKPLELRIRACFDQSLEINGET 120
           AFGP+HHF+P+L KM+LFKL KAKNLKWGPQIH+L+D+L PLEL+IRACFDQ+LEINGET
Sbjct: 61  AFGPFHHFQPELYKMELFKLGKAKNLKWGPQIHKLSDRLTPLELKIRACFDQALEINGET 120

Query: 121 LSWVVLIDGLYLLELLQRRYYSRPLKLPLYIFYGKLWSESEIISDMLKLENQIPLLVLKE 180
           LSW++LIDGL+L+ LLQ  Y + PL  P  IFYGKLWSE EI+ DM+KLENQIPL VL E
Sbjct: 121 LSWLLLIDGLFLIYLLQNEYMNCPLTFPSEIFYGKLWSEFEIVCDMVKLENQIPLFVLNE 180

Query: 181 ILSKDEENDLTSYLYKFSTSVSSFELPHLLTREEILVFYSNIPEINDLSHHLLHFLYFSV 240
           I  ++  N L   LY+F  SVS F+LP    R E   F S +PEI DLSHHLLHFLY  +
Sbjct: 181 ICPEEPINFLAPSLYQFCVSVSPFQLPCFNPRLECFGFSSYLPEIFDLSHHLLHFLYSLI 240

Query: 241 LLIPYDKYEAM-----LKECTNSASSPRYLQH-FGVFSS--HIAFIQQIKQTYGIVQRLL 300
           LLIP ++   +     +   ++S+SS  +L     +  S  +IAFIQQIK+T G++QRLL
Sbjct: 241 LLIPNERVVFVAHCFRMDNSSSSSSSVEFLSECLDILGSVINIAFIQQIKETIGLIQRLL 300

Query: 301 RLLGYVAKLKLNEKQIIPLIPSASDLKKSGFTFKSTKNGILKCSFDETTLTLTLSSIHLD 360
           RLL ++ K  L EK  + +IPSASDLK +GFTFKSTKNGILK +FDETTLTLTL  IHLD
Sbjct: 301 RLLSFITKPNLAEKTPLLIIPSASDLKSAGFTFKSTKNGILKSNFDETTLTLTLPCIHLD 360

Query: 361 GFSQVLLRNLVAFEALAELNPPCLIQYIALMNGLLTNSNDLEILKKAGIVHNHLNSEEEA 420
           GF+ VLL+NLVAFEA+AELNPPCL  Y ALMNGLL NS DL++L+KA IVHNHLNSEEEA
Sbjct: 361 GFTHVLLKNLVAFEAMAELNPPCLANYTALMNGLLRNSKDLKVLEKAEIVHNHLNSEEEA 420

Query: 421 VELFHGVNNSISHFKEASMLKIHLQTGLDMEITSKFLQEI-NMYYDMGLKRMVEEINKSY 480
            ELF+GV NS S  K+ S+LKIHL +G + E  SKF+QE+ + Y +MGL  MVE IN  Y
Sbjct: 421 AELFYGVENSTSKLKKGSLLKIHLNSGFNFENGSKFIQEVESSYIEMGLGDMVERINNCY 480

Query: 481 KRCWRSKMKKFVSIGFKCFAILFIVFLVVLVIAQFLCHYLSCPGKIFQTSIASHVM 528
             CWR KMKKFVS+ +KCFA+L +VFL+VLV  +F+C++LSCP   F  + A H M
Sbjct: 481 GNCWRMKMKKFVSVVYKCFAVLVVVFLIVLVTTRFVCNFLSCPFGTFMNTTALHQM 536

BLAST of Tan0004539 vs. NCBI nr
Match: KAG7037156.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 618.6 bits (1594), Expect = 5.0e-173
Identity = 329/536 (61.38%), Postives = 412/536 (76.87%), Query Frame = 0

Query: 3   SNFPIVSATFNQSQWVIQMNELLHRDDLSAASQSLVSVYKLPDSVRDVDPAAYLPRRVAF 62
           SN  I++ T  +S WV+Q+N+L+ R+ L AAS++ +SV++LP+SVRD DPAAYLP+ +A 
Sbjct: 359 SNMSILNGTLQKSLWVLQINDLIDRNALPAASRTPISVFQLPESVRDGDPAAYLPQFIAL 418

Query: 63  GPYHHFRPDLSKMDLFKLEKAKNLKWGPQIHELADQLKPLELRIRACFDQSLEINGETLS 122
           GPYHHFRP++ KMDLFKL KAKNLKW PQ+HELADQLKPLEL+IR CF  SLEI+GETLS
Sbjct: 419 GPYHHFRPEIFKMDLFKLGKAKNLKWRPQLHELADQLKPLELQIRGCFCGSLEIDGETLS 478

Query: 123 WVVLIDGLYLLELLQRRYYSRPLKLPLYIFYGKLWSESEIISDMLKLENQIPLLVLKEIL 182
           WVVLIDGL+L+ LLQ  + +RPLKLPL IFYGKLWSE EI+SDM+KLENQIPLLVLKEI 
Sbjct: 479 WVVLIDGLFLIHLLQNEFSNRPLKLPLEIFYGKLWSEFEIVSDMMKLENQIPLLVLKEIC 538

Query: 183 SKDEENDLTSYLYKFSTSVSSFELPHLLTREEILVFYSNIPEINDLSHHLLHFLYFSVLL 242
             +  N L    Y+FS SVS FEL           F SN+PEI D S+HLLHF+YF +LL
Sbjct: 539 PDEPFNYLAPLFYQFSASVSPFEL-------VCFGFASNLPEIFDSSYHLLHFVYFLILL 598

Query: 243 IPYDKYEAML--------KECTNSASSPRYL-QHFGVFSS--HIAFIQQIKQTYGIVQRL 302
           IP +   A +         + ++S+SS  +L +   +  S  +IAFIQQ+K+T+G++QRL
Sbjct: 599 IPDEMAVANICAYSMPIGMDSSSSSSSVEFLGESLNILGSVINIAFIQQVKETFGLIQRL 658

Query: 303 LRLLGYVAKLKLNEKQIIPLIPSASDLKKSGFTFKSTKNGILKCSFDETTLTLTLSSIHL 362
            RLLG + K  L EK++  +IPSASDLK +G TFKSTKNG+LKC FDE TLTLTL SIHL
Sbjct: 659 FRLLGSITKPNLCEKKLPMIIPSASDLKTAGITFKSTKNGVLKCRFDEKTLTLTLPSIHL 718

Query: 363 DGFSQVLLRNLVAFEALAELNPPCLIQYIALMNGLLTNSNDLEILKKAGIVHNHLNSEEE 422
           DGFS+VLL+NLVAFEA+AELNP CL+ YIALM GLL NS DL+IL KA IV NHL+SEE+
Sbjct: 719 DGFSEVLLKNLVAFEAMAELNPQCLVNYIALMKGLLRNSKDLKILVKAEIVDNHLSSEEQ 778

Query: 423 AVELFHGVNNSISHFKEASMLKIHLQTGLDMEITSKFLQEINMYYDMGLKRMVEEINKSY 482
           AVE+F+GV NS S  K+  +L IHL++GLD EI S F+Q+I M Y+MGL  MVEEINK Y
Sbjct: 779 AVEVFNGVENSTSSLKKEEVLNIHLKSGLDCEIGSGFIQDIKMNYEMGLGSMVEEINKWY 838

Query: 483 KRCWRSKMKKFVSIGFKCFAILFIVFLVVLVIAQFLCHYLSCPGKIFQTSIASHVM 528
             CWR KMKKF+S+ FKCFA+L +VF++VLVIA+F+C+++SC   +F+TS A H M
Sbjct: 839 GSCWRMKMKKFMSVVFKCFAVLVVVFVIVLVIARFVCNFVSCSVGMFKTSDALHQM 887

BLAST of Tan0004539 vs. NCBI nr
Match: KAA0039249.1 (putative UPF0481 protein [Cucumis melo var. makuwa] >TYK00435.1 putative UPF0481 protein [Cucumis melo var. makuwa])

HSP 1 Score: 616.3 bits (1588), Expect = 2.5e-172
Identity = 323/534 (60.49%), Postives = 404/534 (75.66%), Query Frame = 0

Query: 1   MASNFPIVSATFNQSQWVIQMNELLHRDDLSAASQSLVSVYKLPDSVRDVDPAAYLPRRV 60
           MAS F I++ TF++SQWV+Q+N+L+ ++ L  A+Q+ +S+++LP+SV D DP AYLPR V
Sbjct: 1   MASGFSILTGTFDKSQWVLQINDLVDQNALLDATQTPISIFRLPESVHDGDPTAYLPRHV 60

Query: 61  AFGPYHHFRPDLSKMDLFKLEKAKNLKWGPQIHELADQLKPLELRIRACFDQSLEINGET 120
           AFGP+HHF+P+L KM+LFKL KAKNLKWGPQIH+LAD+L PLEL++RACFDQ+LEINGET
Sbjct: 61  AFGPFHHFQPELYKMELFKLGKAKNLKWGPQIHKLADRLTPLELKMRACFDQALEINGET 120

Query: 121 LSWVVLIDGLYLLELLQRRYYSRPLKLPLYIFYGKLWSESEIISDMLKLENQIPLLVLKE 180
           LSWV+LIDGL+L+ LLQ +Y +RPL+ P  IFYGKLWSE EI+ DM+KLENQIPL VL E
Sbjct: 121 LSWVLLIDGLFLIYLLQNKYMNRPLRFPSEIFYGKLWSEFEIVGDMVKLENQIPLFVLNE 180

Query: 181 ILSKDEENDLTSYLYKFSTSVSSFELPHLLTREEILVFYSNIPEINDLSHHLLHFLYFSV 240
           I  K+  N L  +LY+F  SVSSF LP    + E   F S +PEI DLSHHLLHFLY  +
Sbjct: 181 ICPKEPINFLAPFLYQFCVSVSSFPLPRFNPQLECFGFSSYLPEIFDLSHHLLHFLYSLI 240

Query: 241 LLIPYDKYEAMLKEC----TNSASSPRYL-QHFGVFSS--HIAFIQQIKQTYGIVQRLLR 300
           LLIP D+  A +  C     +S+SS  +L +   +  S  +IAFIQQIK+T G++QRLLR
Sbjct: 241 LLIP-DERVAFMPYCFRLDNSSSSSVEFLSESLDILGSVINIAFIQQIKETIGLIQRLLR 300

Query: 301 LLGYVAKLKLNEKQIIPLIPSASDLKKSGFTFKSTKNGILKCSFDETTLTLTLSSIHLDG 360
           LL  + K  L EK    +IPSASDLK +GFTFKSTKNGILK  FDETTLTLTL  IHL G
Sbjct: 301 LLSSITKPNLAEKTPPLIIPSASDLKSAGFTFKSTKNGILKSYFDETTLTLTLPCIHLHG 360

Query: 361 FSQVLLRNLVAFEALAELNPPCLIQYIALMNGLLTNSNDLEILKKAGIVHNHLNSEEEAV 420
           F+ VLL+NLVAFE++AELNPPCL  Y ALMNGLL  S DL++L+KA IVHNHLNSEEEA 
Sbjct: 361 FTHVLLKNLVAFESMAELNPPCLANYTALMNGLLRKSKDLKVLEKAEIVHNHLNSEEEAA 420

Query: 421 ELFHGVNNSISHFKEASMLKIHLQTGLDMEITSKFLQEINMYYDMGLKRMVEEINKSYKR 480
           ELF+GV NS S  K+ S+L+IHL+ G + E  S F+ E+   Y+MGL  MVE IN  Y  
Sbjct: 421 ELFYGVENSTSRLKKESLLEIHLKGGFNFENGSNFIPEVESSYEMGLGNMVERINNCYGN 480

Query: 481 CWRSKMKKFVSIGFKCFAILFIVFLVVLVIAQFLCHYLSCPGKIFQTSIASHVM 528
           CWR KMKK VS+ +KCFA+L +VFL+VLV  +F+C+++SCP   F T+ A H M
Sbjct: 481 CWRMKMKKVVSVVYKCFAVLVVVFLIVLVTTRFVCNFMSCPLGTFMTTTALHQM 533

BLAST of Tan0004539 vs. NCBI nr
Match: KAG6607499.1 (putative UPF0481 protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 614.8 bits (1584), Expect = 7.2e-172
Identity = 328/533 (61.54%), Postives = 409/533 (76.74%), Query Frame = 0

Query: 7   IVSATFNQSQWVIQMNELLHRDDLSAASQSLVSVYKLPDSVRDVDPAAYLPRRVAFGPYH 66
           I++ T  +S WV+Q+N+L+ R+ L AAS++ +SV++LP+SVRD DPAAYLP+ +A GPYH
Sbjct: 3   ILNGTLQKSLWVLQINDLIDRNALPAASRTPISVFQLPESVRDGDPAAYLPQFIALGPYH 62

Query: 67  HFRPDLSKMDLFKLEKAKNLKWGPQIHELADQLKPLELRIRACFDQSLEINGETLSWVVL 126
           HFRP+L KMDLFKL KAKNLKW PQ+HELADQLKPLEL+IR CF  SLEI+GETLSWVVL
Sbjct: 63  HFRPELFKMDLFKLGKAKNLKWRPQLHELADQLKPLELQIRGCFCGSLEIDGETLSWVVL 122

Query: 127 IDGLYLLELLQRRYYSRPLKLPLYIFYGKLWSESEIISDMLKLENQIPLLVLKEILSKDE 186
           IDGL+L+ LLQ  + +RPLKLPL IFYGKLWSE EI+SDM+KLENQIPLLVLKEI   + 
Sbjct: 123 IDGLFLIHLLQNEFSNRPLKLPLEIFYGKLWSEFEIVSDMMKLENQIPLLVLKEICPDEP 182

Query: 187 ENDLTSYLYKFSTSVSSFELPHLLTREEILVFYSNIPEINDLSHHLLHFLYFSVLLIPYD 246
            N L    Y+FS SVS FEL           F SN+PEI D S+HLLHF+YF +LLIP +
Sbjct: 183 FNYLAPLFYQFSASVSPFEL-------VCFGFASNLPEIFDSSYHLLHFVYFLILLIPDE 242

Query: 247 KYEAM---------LKECTNSASSPRYL-QHFGVFSS--HIAFIQQIKQTYGIVQRLLRL 306
              A          +   ++S+SS  +L +   +  S  +IAFIQQ+K+T+G++QRL RL
Sbjct: 243 MAVANICAYSMPIGMDSSSSSSSSVEFLGESLNILGSVINIAFIQQVKETFGLIQRLFRL 302

Query: 307 LGYVAKLKLNEKQIIPLIPSASDLKKSGFTFKSTKNGILKCSFDETTLTLTLSSIHLDGF 366
           LG + K  L EK++  +IPSASDLK +G TFKSTKNG+LKC FDE TLTLTL SIHLDGF
Sbjct: 303 LGSITKPNLCEKKLPMIIPSASDLKTAGITFKSTKNGVLKCRFDEKTLTLTLPSIHLDGF 362

Query: 367 SQVLLRNLVAFEALAELNPPCLIQYIALMNGLLTNSNDLEILKKAGIVHNHLNSEEEAVE 426
           S+VLL+NLVAFEA+AELNP CL+ YIALM GLL NS DL+IL KA IV NHL+SEE+AVE
Sbjct: 363 SEVLLKNLVAFEAMAELNPQCLVNYIALMKGLLRNSKDLKILVKAEIVDNHLSSEEQAVE 422

Query: 427 LFHGVNNSISHFKEASMLKIHLQTGLDMEITSKFLQEINMYYDMGLKRMVEEINKSYKRC 486
           +F+GV NS S  K+  +L IHL++GLD EI S F+Q+I M Y+MGL  MVEEINK Y  C
Sbjct: 423 VFNGVENSTSSLKKEEVLNIHLKSGLDCEIGSGFIQDIKMNYEMGLGSMVEEINKWYGSC 482

Query: 487 WRSKMKKFVSIGFKCFAILFIVFLVVLVIAQFLCHYLSCPGKIFQTSIASHVM 528
           WR KMKKF+S+ FKCFA+L +VF++VLVIA+F+C+++SC   +F+TS A H M
Sbjct: 483 WRMKMKKFMSVVFKCFAVLVVVFVIVLVIARFVCNFVSCSVGMFKTSDALHQM 528

BLAST of Tan0004539 vs. ExPASy TrEMBL
Match: A0A0A0KSX9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G650580 PE=4 SV=1)

HSP 1 Score: 622.5 bits (1604), Expect = 1.7e-174
Identity = 328/536 (61.19%), Postives = 407/536 (75.93%), Query Frame = 0

Query: 1   MASNFPIVSATFNQSQWVIQMNELLHRDDLSAASQSLVSVYKLPDSVRDVDPAAYLPRRV 60
           MAS F I++ TF++SQW+ Q+N L+++D L AASQ  +SV++LP+SV DVDP AYLPR V
Sbjct: 1   MASGFSILTGTFDKSQWLHQINNLVNQDALLAASQIPISVFRLPESVHDVDPTAYLPRHV 60

Query: 61  AFGPYHHFRPDLSKMDLFKLEKAKNLKWGPQIHELADQLKPLELRIRACFDQSLEINGET 120
           AFGP+HHF+P+L KM+LFKL KAKNLKWGPQIH+L+D+L PLEL+IRACFDQ+LEINGET
Sbjct: 61  AFGPFHHFQPELYKMELFKLGKAKNLKWGPQIHKLSDRLTPLELKIRACFDQALEINGET 120

Query: 121 LSWVVLIDGLYLLELLQRRYYSRPLKLPLYIFYGKLWSESEIISDMLKLENQIPLLVLKE 180
           LSW++LIDGL+L+ LLQ  Y + PL  P  IFYGKLWSE EI+ DM+KLENQIPL VL E
Sbjct: 121 LSWLLLIDGLFLIYLLQNEYMNCPLTFPSEIFYGKLWSEFEIVCDMVKLENQIPLFVLNE 180

Query: 181 ILSKDEENDLTSYLYKFSTSVSSFELPHLLTREEILVFYSNIPEINDLSHHLLHFLYFSV 240
           I  ++  N L   LY+F  SVS F+LP    R E   F S +PEI DLSHHLLHFLY  +
Sbjct: 181 ICPEEPINFLAPSLYQFCVSVSPFQLPCFNPRLECFGFSSYLPEIFDLSHHLLHFLYSLI 240

Query: 241 LLIPYDKYEAM-----LKECTNSASSPRYLQH-FGVFSS--HIAFIQQIKQTYGIVQRLL 300
           LLIP ++   +     +   ++S+SS  +L     +  S  +IAFIQQIK+T G++QRLL
Sbjct: 241 LLIPNERVVFVAHCFRMDNSSSSSSSVEFLSECLDILGSVINIAFIQQIKETIGLIQRLL 300

Query: 301 RLLGYVAKLKLNEKQIIPLIPSASDLKKSGFTFKSTKNGILKCSFDETTLTLTLSSIHLD 360
           RLL ++ K  L EK  + +IPSASDLK +GFTFKSTKNGILK +FDETTLTLTL  IHLD
Sbjct: 301 RLLSFITKPNLAEKTPLLIIPSASDLKSAGFTFKSTKNGILKSNFDETTLTLTLPCIHLD 360

Query: 361 GFSQVLLRNLVAFEALAELNPPCLIQYIALMNGLLTNSNDLEILKKAGIVHNHLNSEEEA 420
           GF+ VLL+NLVAFEA+AELNPPCL  Y ALMNGLL NS DL++L+KA IVHNHLNSEEEA
Sbjct: 361 GFTHVLLKNLVAFEAMAELNPPCLANYTALMNGLLRNSKDLKVLEKAEIVHNHLNSEEEA 420

Query: 421 VELFHGVNNSISHFKEASMLKIHLQTGLDMEITSKFLQEI-NMYYDMGLKRMVEEINKSY 480
            ELF+GV NS S  K+ S+LKIHL +G + E  SKF+QE+ + Y +MGL  MVE IN  Y
Sbjct: 421 AELFYGVENSTSKLKKGSLLKIHLNSGFNFENGSKFIQEVESSYIEMGLGDMVERINNCY 480

Query: 481 KRCWRSKMKKFVSIGFKCFAILFIVFLVVLVIAQFLCHYLSCPGKIFQTSIASHVM 528
             CWR KMKKFVS+ +KCFA+L +VFL+VLV  +F+C++LSCP   F  + A H M
Sbjct: 481 GNCWRMKMKKFVSVVYKCFAVLVVVFLIVLVTTRFVCNFLSCPFGTFMNTTALHQM 536

BLAST of Tan0004539 vs. ExPASy TrEMBL
Match: A0A5D3BMY1 (Putative UPF0481 protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G00380 PE=4 SV=1)

HSP 1 Score: 616.3 bits (1588), Expect = 1.2e-172
Identity = 323/534 (60.49%), Postives = 404/534 (75.66%), Query Frame = 0

Query: 1   MASNFPIVSATFNQSQWVIQMNELLHRDDLSAASQSLVSVYKLPDSVRDVDPAAYLPRRV 60
           MAS F I++ TF++SQWV+Q+N+L+ ++ L  A+Q+ +S+++LP+SV D DP AYLPR V
Sbjct: 1   MASGFSILTGTFDKSQWVLQINDLVDQNALLDATQTPISIFRLPESVHDGDPTAYLPRHV 60

Query: 61  AFGPYHHFRPDLSKMDLFKLEKAKNLKWGPQIHELADQLKPLELRIRACFDQSLEINGET 120
           AFGP+HHF+P+L KM+LFKL KAKNLKWGPQIH+LAD+L PLEL++RACFDQ+LEINGET
Sbjct: 61  AFGPFHHFQPELYKMELFKLGKAKNLKWGPQIHKLADRLTPLELKMRACFDQALEINGET 120

Query: 121 LSWVVLIDGLYLLELLQRRYYSRPLKLPLYIFYGKLWSESEIISDMLKLENQIPLLVLKE 180
           LSWV+LIDGL+L+ LLQ +Y +RPL+ P  IFYGKLWSE EI+ DM+KLENQIPL VL E
Sbjct: 121 LSWVLLIDGLFLIYLLQNKYMNRPLRFPSEIFYGKLWSEFEIVGDMVKLENQIPLFVLNE 180

Query: 181 ILSKDEENDLTSYLYKFSTSVSSFELPHLLTREEILVFYSNIPEINDLSHHLLHFLYFSV 240
           I  K+  N L  +LY+F  SVSSF LP    + E   F S +PEI DLSHHLLHFLY  +
Sbjct: 181 ICPKEPINFLAPFLYQFCVSVSSFPLPRFNPQLECFGFSSYLPEIFDLSHHLLHFLYSLI 240

Query: 241 LLIPYDKYEAMLKEC----TNSASSPRYL-QHFGVFSS--HIAFIQQIKQTYGIVQRLLR 300
           LLIP D+  A +  C     +S+SS  +L +   +  S  +IAFIQQIK+T G++QRLLR
Sbjct: 241 LLIP-DERVAFMPYCFRLDNSSSSSVEFLSESLDILGSVINIAFIQQIKETIGLIQRLLR 300

Query: 301 LLGYVAKLKLNEKQIIPLIPSASDLKKSGFTFKSTKNGILKCSFDETTLTLTLSSIHLDG 360
           LL  + K  L EK    +IPSASDLK +GFTFKSTKNGILK  FDETTLTLTL  IHL G
Sbjct: 301 LLSSITKPNLAEKTPPLIIPSASDLKSAGFTFKSTKNGILKSYFDETTLTLTLPCIHLHG 360

Query: 361 FSQVLLRNLVAFEALAELNPPCLIQYIALMNGLLTNSNDLEILKKAGIVHNHLNSEEEAV 420
           F+ VLL+NLVAFE++AELNPPCL  Y ALMNGLL  S DL++L+KA IVHNHLNSEEEA 
Sbjct: 361 FTHVLLKNLVAFESMAELNPPCLANYTALMNGLLRKSKDLKVLEKAEIVHNHLNSEEEAA 420

Query: 421 ELFHGVNNSISHFKEASMLKIHLQTGLDMEITSKFLQEINMYYDMGLKRMVEEINKSYKR 480
           ELF+GV NS S  K+ S+L+IHL+ G + E  S F+ E+   Y+MGL  MVE IN  Y  
Sbjct: 421 ELFYGVENSTSRLKKESLLEIHLKGGFNFENGSNFIPEVESSYEMGLGNMVERINNCYGN 480

Query: 481 CWRSKMKKFVSIGFKCFAILFIVFLVVLVIAQFLCHYLSCPGKIFQTSIASHVM 528
           CWR KMKK VS+ +KCFA+L +VFL+VLV  +F+C+++SCP   F T+ A H M
Sbjct: 481 CWRMKMKKVVSVVYKCFAVLVVVFLIVLVTTRFVCNFMSCPLGTFMTTTALHQM 533

BLAST of Tan0004539 vs. ExPASy TrEMBL
Match: A0A6J1GBT5 (putative UPF0481 protein At3g02645 OS=Cucurbita moschata OX=3662 GN=LOC111452550 PE=4 SV=1)

HSP 1 Score: 614.4 bits (1583), Expect = 4.6e-172
Identity = 329/530 (62.08%), Postives = 408/530 (76.98%), Query Frame = 0

Query: 7   IVSATFNQSQWVIQMNELLHRDDLSAASQSLVSVYKLPDSVRDVDPAAYLPRRVAFGPYH 66
           I++ T  +SQWV+Q+N+L+ R+ L AAS++ +SV++LP+SVRD DPAAYLP+ +A GPYH
Sbjct: 3   ILNGTLQKSQWVLQINDLIDRNALPAASRTPISVFQLPESVRDGDPAAYLPQFIALGPYH 62

Query: 67  HFRPDLSKMDLFKLEKAKNLKWGPQIHELADQLKPLELRIRACFDQSLEINGETLSWVVL 126
           HFRP+L KMDLFKL KAKNLKW PQ+HELADQLKPLEL+IR CF  SLEI+ ETLSWVVL
Sbjct: 63  HFRPELYKMDLFKLGKAKNLKWRPQLHELADQLKPLELQIRGCFCGSLEIDDETLSWVVL 122

Query: 127 IDGLYLLELLQRRYYSRPLKLPLYIFYGKLWSESEIISDMLKLENQIPLLVLKEILSKDE 186
           IDGL+L+ LLQ  + +RPLKLPL IFYGKLWSE EI+SDM+KLENQIPLLVLKEI   + 
Sbjct: 123 IDGLFLIHLLQNEFSNRPLKLPLEIFYGKLWSEFEIVSDMMKLENQIPLLVLKEICPDEP 182

Query: 187 ENDLTSYLYKFSTSVSSFELPHLLTREEILVFYSNIPEINDLSHHLLHFLYFSVLLIPYD 246
            N L    Y+FS SVS FEL           F SN+PEI D S+HLLHF+YF +LLIP +
Sbjct: 183 FNYLAPLFYQFSASVSPFEL-------VCFGFASNLPEIFDSSYHLLHFVYFLILLIPDE 242

Query: 247 KYEA------MLKECTNSASSPRYL-QHFGVFSS--HIAFIQQIKQTYGIVQRLLRLLGY 306
              A      M     +S+SS  +L +   +  S  +IAFIQQ+K+T+G++QRL RLLG 
Sbjct: 243 MAVANICAYSMPIGMDSSSSSVEFLGESLNILGSVINIAFIQQVKETFGLIQRLFRLLGS 302

Query: 307 VAKLKLNEKQIIPLIPSASDLKKSGFTFKSTKNGILKCSFDETTLTLTLSSIHLDGFSQV 366
           + K  L EK++  +IPSASDLK +G TFKSTKNG+LKC FDE TLTLTL SIHLDGFS+V
Sbjct: 303 ITKPNLCEKKLPMIIPSASDLKTAGITFKSTKNGVLKCRFDEKTLTLTLPSIHLDGFSEV 362

Query: 367 LLRNLVAFEALAELNPPCLIQYIALMNGLLTNSNDLEILKKAGIVHNHLNSEEEAVELFH 426
           LL+NLVAFEA+AELNP CL+ YIALM GLL NS DL+IL KA IV NHL+SEE+AVE+F+
Sbjct: 363 LLKNLVAFEAMAELNPQCLVNYIALMKGLLRNSKDLKILVKAEIVDNHLSSEEQAVEVFN 422

Query: 427 GVNNSISHFKEASMLKIHLQTGLDMEITSKFLQEINMYYDMGLKRMVEEINKSYKRCWRS 486
           GV NS S  K+  +L IHL++GLD EI S F+Q+I M Y+MGL  MVEEINK Y  CWR 
Sbjct: 423 GVENSTSSLKKEEVLNIHLKSGLDCEIGSGFIQDIKMNYEMGLGSMVEEINKWYGSCWRM 482

Query: 487 KMKKFVSIGFKCFAILFIVFLVVLVIAQFLCHYLSCPGKIFQTSIASHVM 528
           KMKKF+S+ FKCFA+L +VF++VLVIA+F+C+++SC   +F+TS A H M
Sbjct: 483 KMKKFMSVVFKCFAVLVVVFVIVLVIARFVCNFVSCSVGMFKTSDALHQM 525

BLAST of Tan0004539 vs. ExPASy TrEMBL
Match: A0A1S3CAN6 (putative UPF0481 protein At3g02645 OS=Cucumis melo OX=3656 GN=LOC103498704 PE=4 SV=1)

HSP 1 Score: 612.1 bits (1577), Expect = 2.3e-171
Identity = 322/533 (60.41%), Postives = 401/533 (75.23%), Query Frame = 0

Query: 1   MASNFPIVSATFNQSQWVIQMNELLHRDDLSAASQSLVSVYKLPDSVRDVDPAAYLPRRV 60
           MAS F I++ TF++SQWV+Q+N L+ ++ L  A+Q+ +SV++LP+SV D DP AYLPR V
Sbjct: 1   MASGFSILTGTFDKSQWVLQINNLVDQNALLDATQTPISVFRLPESVHDGDPTAYLPRHV 60

Query: 61  AFGPYHHFRPDLSKMDLFKLEKAKNLKWGPQIHELADQLKPLELRIRACFDQSLEINGET 120
           AFGP+HHF+P+L KM+LFKL KAKNLKWGPQIH+LAD+L PLEL++RACFDQ+LEINGET
Sbjct: 61  AFGPFHHFQPELHKMELFKLGKAKNLKWGPQIHKLADRLTPLELKMRACFDQALEINGET 120

Query: 121 LSWVVLIDGLYLLELLQRRYYSRPLKLPLYIFYGKLWSESEIISDMLKLENQIPLLVLKE 180
           LSWV+LIDGL+L+ LLQ +Y + PL+ P  IFYGKLWSE EI+ DM+KLENQIPL VL E
Sbjct: 121 LSWVLLIDGLFLIYLLQNKYMNCPLRFPSEIFYGKLWSEFEIVCDMVKLENQIPLFVLNE 180

Query: 181 ILSKDEENDLTSYLYKFSTSVSSFELPHLLTREEILVFYSNIPEINDLSHHLLHFLYFSV 240
           I  K+  N L  +LY+F  SVS F LP    + E   F S +PEI DLSHHLLHFLY  +
Sbjct: 181 ICPKEPINFLAPFLYQFCVSVSPFPLPRFNPQLECFGFSSYLPEIFDLSHHLLHFLYSLI 240

Query: 241 LLIPYDKYEAM---LKECTNSASSPRYL-QHFGVFSS--HIAFIQQIKQTYGIVQRLLRL 300
           LLIP ++   M   L+   +S+SS  +L +   +  S  +IAFIQQIK+T G++QRLLRL
Sbjct: 241 LLIPDERVVIMLFGLRLDNSSSSSVEFLSESLDILGSVINIAFIQQIKETIGLIQRLLRL 300

Query: 301 LGYVAKLKLNEKQIIPLIPSASDLKKSGFTFKSTKNGILKCSFDETTLTLTLSSIHLDGF 360
           L  + K  L EK    +IPSAS LK +GFTFKSTKNGILK  FDETTLTLTL  IHL GF
Sbjct: 301 LSSITKPNLAEKTPPLIIPSASHLKSAGFTFKSTKNGILKSYFDETTLTLTLPCIHLHGF 360

Query: 361 SQVLLRNLVAFEALAELNPPCLIQYIALMNGLLTNSNDLEILKKAGIVHNHLNSEEEAVE 420
           + VLL+NLVAFE++AELNPPCL  Y ALMNGLL NS DL++L+KA IVHNHLNSEEEA E
Sbjct: 361 THVLLKNLVAFESMAELNPPCLANYTALMNGLLRNSKDLKVLEKAEIVHNHLNSEEEAAE 420

Query: 421 LFHGVNNSISHFKEASMLKIHLQTGLDMEITSKFLQEINMYYDMGLKRMVEEINKSYKRC 480
           LF+GV NS    K+ S+L+IHL+ G + E  S F+QE+   Y+MGL  MVE IN  Y  C
Sbjct: 421 LFYGVENSTRRLKKESLLEIHLKGGFNFENGSNFIQEVESSYEMGLGNMVERINNCYGNC 480

Query: 481 WRSKMKKFVSIGFKCFAILFIVFLVVLVIAQFLCHYLSCPGKIFQTSIASHVM 528
           WR KMKK VS+ FKCFA+L +VFL+VLV  +F+C+++SCP   F T+ A H M
Sbjct: 481 WRMKMKKVVSVVFKCFAVLIVVFLIVLVTTRFVCNFMSCPLGTFMTTTALHQM 533

BLAST of Tan0004539 vs. ExPASy TrEMBL
Match: A0A6J1IBB2 (putative UPF0481 protein At3g02645 OS=Cucurbita maxima OX=3661 GN=LOC111471968 PE=4 SV=1)

HSP 1 Score: 608.6 bits (1568), Expect = 2.5e-170
Identity = 323/531 (60.83%), Postives = 407/531 (76.65%), Query Frame = 0

Query: 7   IVSATFNQSQWVIQMNELLHRDDLSAASQSLVSVYKLPDSVRDVDPAAYLPRRVAFGPYH 66
           I++ T  +SQWV+Q+ +++H++ L AAS + +SV++LP+SVRD DPAAYLP+ +A GPYH
Sbjct: 3   ILNGTLEKSQWVLQIFDIIHQNALPAASHTPISVFQLPESVRDGDPAAYLPQFIALGPYH 62

Query: 67  HFRPDLSKMDLFKLEKAKNLKWGPQIHELADQLKPLELRIRACFDQSLEINGETLSWVVL 126
           HFR +L KMDLFKL KAKNLKW PQ+HELADQLKPLEL+IR CF  SLEI+GETLSWVVL
Sbjct: 63  HFRSELYKMDLFKLGKAKNLKWRPQLHELADQLKPLELQIRGCFCGSLEIDGETLSWVVL 122

Query: 127 IDGLYLLELLQRRYYSRPLKLPLYIFYGKLWSESEIISDMLKLENQIPLLVLKEILSKDE 186
           IDGL+L++LLQ  + +RPLKLPL IFYGKLWSE EI+SDM+KLENQIPLLVL+EI   + 
Sbjct: 123 IDGLFLIDLLQNEFSNRPLKLPLEIFYGKLWSEFEIVSDMVKLENQIPLLVLQEICPDEP 182

Query: 187 ENDLTSYLYKFSTSVSSFELPHLLTREEILVFYSNIPEINDLSHHLLHFLYFSVLLIPYD 246
            N L    Y+FS SVS FEL           F SN+PEI D SHHLLHF+YF +LLIP +
Sbjct: 183 FNYLAPLFYQFSASVSPFEL-------VCFGFASNLPEIFDSSHHLLHFVYFLILLIPDE 242

Query: 247 -------KYEAMLKECTNSASSPRYL-QHFGVFSS--HIAFIQQIKQTYGIVQRLLRLLG 306
                   Y   +   ++S+SS   L +   +  S   IAFIQQ+K+T+G+++RL RLLG
Sbjct: 243 MAVANICAYSMPIGMDSSSSSSVELLGESLNILGSVISIAFIQQVKETFGLIRRLFRLLG 302

Query: 307 YVAKLKLNEKQIIPLIPSASDLKKSGFTFKSTKNGILKCSFDETTLTLTLSSIHLDGFSQ 366
            + K  L EK++  +IPSASDLK +G TFKST NG+LKC FDE TLTLTL SIHLD FS+
Sbjct: 303 SITKPNLCEKKLPMIIPSASDLKTAGITFKSTNNGVLKCRFDEKTLTLTLPSIHLDWFSE 362

Query: 367 VLLRNLVAFEALAELNPPCLIQYIALMNGLLTNSNDLEILKKAGIVHNHLNSEEEAVELF 426
           VLL+NLVAFEA+AELNP CL+ YIALM GLL NS DL+IL+KA IVHNHL++EE+AVE+F
Sbjct: 363 VLLKNLVAFEAMAELNPQCLVNYIALMKGLLRNSKDLKILEKAEIVHNHLSNEEQAVEVF 422

Query: 427 HGVNNSISHFKEASMLKIHLQTGLDMEITSKFLQEINMYYDMGLKRMVEEINKSYKRCWR 486
           +GV NS S  K+  +LKIHL++GLD EI S F+Q+I M Y+MGL  MVEEINK Y  CWR
Sbjct: 423 NGVENSTSSLKKEEVLKIHLKSGLDCEIGSGFIQDIKMNYEMGLGSMVEEINKWYGSCWR 482

Query: 487 SKMKKFVSIGFKCFAILFIVFLVVLVIAQFLCHYLSCPGKIFQTSIASHVM 528
            KMK+F+S+ FKCFA+L +VF++VLVIA+F C+++SC   +F+TS A H M
Sbjct: 483 MKMKRFMSVVFKCFAVLVVVFVIVLVIARFACNFVSCSVGMFKTSDALHQM 526

BLAST of Tan0004539 vs. TAIR 10
Match: AT3G02645.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 156.4 bits (394), Expect = 6.5e-38
Identity = 147/560 (26.25%), Postives = 267/560 (47.68%), Query Frame = 0

Query: 6   PIVSAT----FNQSQWVIQMNELLHRD-DLSAASQSLVSVYKLPDSVRDVDPAAYLPRRV 65
           PI S+T    F++++WVI + + L  + +     +  VS++ +P ++    P +Y P RV
Sbjct: 6   PIFSSTEQHRFDETRWVINVQKSLDAELEEHDLEEVTVSIFNVPKALMCSHPDSYTPHRV 65

Query: 66  AFGPYHHFRPDLSKMDLFKL---EKAKNLKWGPQIHELADQLKPLELRIRACFDQSLEIN 125
           + GPYH  +P+L +M+ +KL    K +N     + H+L ++L+ +E++IRAC+ + +  N
Sbjct: 66  SIGPYHCLKPELHEMERYKLMIARKIRNQYNSFRFHDLVEKLQSMEIKIRACYHKYIGFN 125

Query: 126 GETLSWVVLIDGLYLLELLQRRYYSRPLKLPLYIFYGKLWSESEIISDMLKLENQIPLLV 185
           GETL W++ +D  +L+E L+   + +   L   +        +EI+ D++ +ENQIPL V
Sbjct: 126 GETLLWIMAVDSSFLIEFLKIYSFRKVETLINRV------GHNEILRDIMMIENQIPLFV 185

Query: 186 LKEIL-----SKDEENDLTSYLYKFSTSVSSFELPHLLTREEILVFYSNIPEINDLSHHL 245
           L++ L     S +  +DL   L    T +     P ++  ++  +  +   E N    H+
Sbjct: 186 LRKTLEFQLESTESADDL---LLSVLTGLCKDLSPLVIKFDDDQILKAQFQECN----HI 245

Query: 246 LHFLYFSVLLIPYDKYEAMLKECTNSASSPRYLQHFGVFSSHIAFIQQIKQTYGIV---- 305
           L FLY   +++P  + E    E        R  ++ G  +  I F+ +IK  +  V    
Sbjct: 246 LDFLY--QMIVPRIEEE----ELEEDDEENRADENGG--NRAIRFMDEIKHQFKRVFASR 305

Query: 306 ----------QRLLRLLGYVAKLKLNEKQII-----------------------PL---- 365
                     + +  L G++A LKL+   +                        PL    
Sbjct: 306 PADLILRFPWRIISNLPGFMA-LKLSADYLFTRQENEATTTRQESVSILDIEKPPLVEEL 365

Query: 366 -IPSASDLKKSGFTFKSTKNG-ILKCSFDETTLTLTLSSIHLDGFSQVLLRNLVAFEALA 425
            IPS SDL K+G  FK T +G I   +FD  +    L  I+LD  ++ +LRNLVA+EA  
Sbjct: 366 TIPSVSDLHKAGVRFKPTAHGNISTVTFDSNSGQFYLPVINLDINTETVLRNLVAYEATN 425

Query: 426 ELNPPCLIQYIALMNGLLTNSNDLEILKKAGIVHNHLNSEEEAVELFHGVNNSISHFKEA 485
              P    +Y  L+NG++ +  D+ +L++ G++ + L S++EA E+++G++ S+      
Sbjct: 426 TSGPLVFTRYTELINGIIDSEEDVRLLREQGVLVSRLKSDQEAAEMWNGMSKSVR----- 485

Query: 486 SMLKIHLQTGLDMEITSKFLQEINMYYDMGLKRMVEEINKSYKRCWRSKMKKFVSI---- 506
                              L ++       L + +E++N+ Y   W+ K+ + V +    
Sbjct: 486 -------------------LTKVGF-----LDKTIEDVNRYYTGRWKVKIGRLVEVYVYG 514

BLAST of Tan0004539 vs. TAIR 10
Match: AT4G31980.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF247, plant (InterPro:IPR004158), Protein of unknown function DUF862, eukaryotic (InterPro:IPR008580); BEST Arabidopsis thaliana protein match is: Plant protein of unknown function (DUF247) (TAIR:AT5G11290.1); Has 1967 Blast hits to 1844 proteins in 183 species: Archae - 0; Bacteria - 6; Metazoa - 223; Fungi - 83; Plants - 1477; Viruses - 0; Other Eukaryotes - 178 (source: NCBI BLink). )

HSP 1 Score: 91.3 bits (225), Expect = 2.6e-18
Identity = 92/404 (22.77%), Postives = 176/404 (43.56%), Query Frame = 0

Query: 40  VYKLPDSVRDVDPAAYLPRRVAFGPYHHFRPDLSKMDLFKLEKAKNL--KWGPQIHELAD 99
           +YK+P+ +R ++P AY PR V+FGP H  + +L  M+  K     +   +    + +L  
Sbjct: 295 IYKVPNKLRRLNPDAYTPRLVSFGPLHRGKEELQAMEDQKYRYLLSFIPRTNSSLEDLVR 354

Query: 100 QLKPLELRIRACFDQSLEINGETLSWVVLIDGLYLLELLQRRYYSRPLKLPLYIFYGKLW 159
             +  E   R+C+ + ++++ +    ++++DG +L+ELL R +Y R L+      +G   
Sbjct: 355 LARTWEQNARSCYAEDVKLHSDEFVEMLVVDGSFLVELLLRSHYPR-LRGENDRIFGNSM 414

Query: 160 SESEIISDMLKLENQIPLLVLKEILSKDEENDLTSYLYKFSTSVSSFELPHLLTREEILV 219
             +++  DM+ +ENQ+P  V+KEI        L +Y  + + S+        L +     
Sbjct: 415 MITDVCRDMILIENQLPFFVVKEIFLL-----LLNYYQQGTPSIIQ------LAQRHFSY 474

Query: 220 FYSNIPEINDLSHHLLHFLYFSVLLIPYDKYEAMLKECTNSASSPRYLQHFGVFSSHIAF 279
           F S I +                 +   + +  +L+ C        YL  F         
Sbjct: 475 FLSRIDD--------------EKFITEPEHFVDLLRSC--------YLPQF--------- 534

Query: 280 IQQIKQTYGIVQRLLRLLGYVAKLKLNEKQIIPLIPSASDLKKSGFTFK--STKNGILKC 339
              IK  Y  V+                   +   P A++L  +G  FK   T + +L  
Sbjct: 535 --PIKLEYTTVK-------------------VDNAPEATELHTAGVRFKPAETSSCLLDI 594

Query: 340 SFDETTLTLTLSSIHLDGFSQVLLRNLVAFEALAELNPPCLIQYIALMNGLLTNSNDLEI 399
           SF +    L + +I +D  ++ L +N++ FE     N    + YI L+   + +  D ++
Sbjct: 595 SFADG--VLKIPTIVVDDLTESLYKNIIGFEQCRCSNKN-FLDYIMLLGCFIKSPTDADL 631

Query: 400 LKKAGIVHNHLNSEEEAVELFHGVNNSISHFKE--ASMLKIHLQ 438
           L  +GI+ N+L +  +   LF+ ++  + + +    SML  +LQ
Sbjct: 655 LIHSGIIVNYLGNSVDVSNLFNSISKEVIYDRRFYFSMLSENLQ 631

BLAST of Tan0004539 vs. TAIR 10
Match: AT3G47250.1 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 82.0 bits (201), Expect = 1.6e-15
Identity = 101/415 (24.34%), Postives = 163/415 (39.28%), Query Frame = 0

Query: 30  LSAASQSLVSVYKLPDSVRDVDPAAYLPRRVAFGPYHHFRPDLS-----KMDLFKLEKAK 89
           L +A ++   ++++PDS+ +V+P AY P+ V+ GPYH+    L      K    +L   +
Sbjct: 53  LESAGKASCCIFRIPDSLAEVNPKAYKPKVVSIGPYHYGENHLQMIQQHKFRFLELFVDR 112

Query: 90  NLKWGPQIHELADQLKPLELRIRACFDQSLEINGETLSWVVLIDGLYLLELL---QRRYY 149
             K G   + L   +  L+ +IRA + + L I    L  ++++DG ++L LL    R+  
Sbjct: 113 ATKKGMDENVLYAAVGALQHKIRASYSEELRIEKSELVSMMILDGCFILMLLLIVSRKID 172

Query: 150 SRPLKLPLYIFYGKLWSESEIISDMLKLENQIPLLVLKEILSKDEENDLTSYLYKFSTSV 209
               K P++      W  + I SD+L LENQ+P  VL+ I  K                 
Sbjct: 173 LDMNKDPIFTI---PWILASIQSDLLLLENQVPFFVLRTIFDK----------------- 232

Query: 210 SSFELPHLLTREEILVFYSNIPEINDLSHHLLHFLYFSVLLIPYDKYEAMLKECTNSASS 269
                             S I    DL+   + F +F++ +   D Y A  K     A  
Sbjct: 233 ------------------SGIGSPGDLNR--MAFSFFNLSIDKPDTYWA--KHRDRGAKH 292

Query: 270 PRYLQHFGVFSSHIAFIQQIKQTYGIVQRLLRLLGYVAKLKLNEKQIIPLIPSASDLKKS 329
              L       S  +  +         Q      G V+    + +   PLI SA  L+  
Sbjct: 293 LLDLNRKTFIPSMRSMGESETTPSSKFQHSKGKSGEVS----SSESTFPLILSAKRLRLQ 352

Query: 330 G--FTFKSTKNGILKCSFDETTLTLTLSSIHLDGFSQVLLRNLVAFEALAELNPPCLIQY 389
           G  F  +S    IL     +  L + L  + LDGF   +  N VAFE     +   +  Y
Sbjct: 353 GIKFRLRSDAESILDIKLKKNKLQIPL--LRLDGFISSIFLNCVAFEQFYTESTNDITSY 412

Query: 390 IALMNGLLTNSNDLEILKK-AGIVHNHLNSEEEAVELFHGVNNSISHFKEASMLK 434
           +  M  LL +  D   L     I+ N+  +E E  + F  +   +      S L+
Sbjct: 413 VVFMGCLLNDQEDATFLNNDKRIIENYFGNENEVSQFFKTICKDVVFDTRRSYLR 419

BLAST of Tan0004539 vs. TAIR 10
Match: AT3G47250.2 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 82.0 bits (201), Expect = 1.6e-15
Identity = 101/415 (24.34%), Postives = 163/415 (39.28%), Query Frame = 0

Query: 30  LSAASQSLVSVYKLPDSVRDVDPAAYLPRRVAFGPYHHFRPDLS-----KMDLFKLEKAK 89
           L +A ++   ++++PDS+ +V+P AY P+ V+ GPYH+    L      K    +L   +
Sbjct: 53  LESAGKASCCIFRIPDSLAEVNPKAYKPKVVSIGPYHYGENHLQMIQQHKFRFLELFVDR 112

Query: 90  NLKWGPQIHELADQLKPLELRIRACFDQSLEINGETLSWVVLIDGLYLLELL---QRRYY 149
             K G   + L   +  L+ +IRA + + L I    L  ++++DG ++L LL    R+  
Sbjct: 113 ATKKGMDENVLYAAVGALQHKIRASYSEELRIEKSELVSMMILDGCFILMLLLIVSRKID 172

Query: 150 SRPLKLPLYIFYGKLWSESEIISDMLKLENQIPLLVLKEILSKDEENDLTSYLYKFSTSV 209
               K P++      W  + I SD+L LENQ+P  VL+ I  K                 
Sbjct: 173 LDMNKDPIFTI---PWILASIQSDLLLLENQVPFFVLRTIFDK----------------- 232

Query: 210 SSFELPHLLTREEILVFYSNIPEINDLSHHLLHFLYFSVLLIPYDKYEAMLKECTNSASS 269
                             S I    DL+   + F +F++ +   D Y A  K     A  
Sbjct: 233 ------------------SGIGSPGDLNR--MAFSFFNLSIDKPDTYWA--KHRDRGAKH 292

Query: 270 PRYLQHFGVFSSHIAFIQQIKQTYGIVQRLLRLLGYVAKLKLNEKQIIPLIPSASDLKKS 329
              L       S  +  +         Q      G V+    + +   PLI SA  L+  
Sbjct: 293 LLDLNRKTFIPSMRSMGESETTPSSKFQHSKGKSGEVS----SSESTFPLILSAKRLRLQ 352

Query: 330 G--FTFKSTKNGILKCSFDETTLTLTLSSIHLDGFSQVLLRNLVAFEALAELNPPCLIQY 389
           G  F  +S    IL     +  L + L  + LDGF   +  N VAFE     +   +  Y
Sbjct: 353 GIKFRLRSDAESILDIKLKKNKLQIPL--LRLDGFISSIFLNCVAFEQFYTESTNDITSY 412

Query: 390 IALMNGLLTNSNDLEILKK-AGIVHNHLNSEEEAVELFHGVNNSISHFKEASMLK 434
           +  M  LL +  D   L     I+ N+  +E E  + F  +   +      S L+
Sbjct: 413 VVFMGCLLNDQEDATFLNNDKRIIENYFGNENEVSQFFKTICKDVVFDTRRSYLR 419

BLAST of Tan0004539 vs. TAIR 10
Match: AT3G47250.3 (Plant protein of unknown function (DUF247) )

HSP 1 Score: 82.0 bits (201), Expect = 1.6e-15
Identity = 101/415 (24.34%), Postives = 163/415 (39.28%), Query Frame = 0

Query: 30  LSAASQSLVSVYKLPDSVRDVDPAAYLPRRVAFGPYHHFRPDLS-----KMDLFKLEKAK 89
           L +A ++   ++++PDS+ +V+P AY P+ V+ GPYH+    L      K    +L   +
Sbjct: 53  LESAGKASCCIFRIPDSLAEVNPKAYKPKVVSIGPYHYGENHLQMIQQHKFRFLELFVDR 112

Query: 90  NLKWGPQIHELADQLKPLELRIRACFDQSLEINGETLSWVVLIDGLYLLELL---QRRYY 149
             K G   + L   +  L+ +IRA + + L I    L  ++++DG ++L LL    R+  
Sbjct: 113 ATKKGMDENVLYAAVGALQHKIRASYSEELRIEKSELVSMMILDGCFILMLLLIVSRKID 172

Query: 150 SRPLKLPLYIFYGKLWSESEIISDMLKLENQIPLLVLKEILSKDEENDLTSYLYKFSTSV 209
               K P++      W  + I SD+L LENQ+P  VL+ I  K                 
Sbjct: 173 LDMNKDPIFTI---PWILASIQSDLLLLENQVPFFVLRTIFDK----------------- 232

Query: 210 SSFELPHLLTREEILVFYSNIPEINDLSHHLLHFLYFSVLLIPYDKYEAMLKECTNSASS 269
                             S I    DL+   + F +F++ +   D Y A  K     A  
Sbjct: 233 ------------------SGIGSPGDLNR--MAFSFFNLSIDKPDTYWA--KHRDRGAKH 292

Query: 270 PRYLQHFGVFSSHIAFIQQIKQTYGIVQRLLRLLGYVAKLKLNEKQIIPLIPSASDLKKS 329
              L       S  +  +         Q      G V+    + +   PLI SA  L+  
Sbjct: 293 LLDLNRKTFIPSMRSMGESETTPSSKFQHSKGKSGEVS----SSESTFPLILSAKRLRLQ 352

Query: 330 G--FTFKSTKNGILKCSFDETTLTLTLSSIHLDGFSQVLLRNLVAFEALAELNPPCLIQY 389
           G  F  +S    IL     +  L + L  + LDGF   +  N VAFE     +   +  Y
Sbjct: 353 GIKFRLRSDAESILDIKLKKNKLQIPL--LRLDGFISSIFLNCVAFEQFYTESTNDITSY 412

Query: 390 IALMNGLLTNSNDLEILKK-AGIVHNHLNSEEEAVELFHGVNNSISHFKEASMLK 434
           +  M  LL +  D   L     I+ N+  +E E  + F  +   +      S L+
Sbjct: 413 VVFMGCLLNDQEDATFLNNDKRIIENYFGNENEVSQFFKTICKDVVFDTRRSYLR 419