Tan0004530 (gene) Snake gourd v1

Overview
NameTan0004530
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionEukaryotic peptide chain release factor subunit 1-1
LocationLG06: 18821676 .. 18824502 (-)
RNA-Seq ExpressionTan0004530
SyntenyTan0004530
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCAGCCCGACCTCTTCCAGCCCAACAACTTTTCCCCCAACTCATGTAGAAACCCTAAATCTCACAAATTCAATCTCTCATTTTCAATCCTAGATCTCTCTCGTCTCTCCCGAGCAGAGTCGTTTGCGGCCCCTCTCCTCCGATTGCTTACCCAAGTTAGCAACTTGCCATCTTCAATCTCTCAGATCTCTTCCGGTGCTCCGAGATCTCTCTCGCTAATATCTTCCCGGCGGCATCTCTCACGAGCATCATCCTAGCGGCCGGCGGCTGCTCTCCCTCACGAACACCCATCGCGGCGGTATCCTTCTCTCTTGAGCACCCATCCCGCCGGTACCCCCCTCTCACTTTTATAATAATTTCTTGTGTTAATGAGATGATATGGAAACGGTCTGGTGGAAAATCAATTTTGTTTCTCTGTTGTCTCTCTTTGAATTTTAATTTAATTACATATCCTGCATAACCTCTCATGGAAACAATAGAAGTTTCTTGATCCAATTCATCCCCTCAGGTAATTTTCTTTTTCCTGCAGTACTCTCATTTATTACGATGGCTGACGGTCATGAAACTGATAAGAACATTGAAATATGGAAAATTAAGAAGTTGATTAAAGCACTTGAAGCTGCTAGGGGAAATGGGACTAGCATGATTTCTCTCATTATGCCTCCTCGTGATCAAATATCTCGTGTTACTAAGATGCTTGGTGATGAATTTGGAACTGCTTCGAACATTAAGAGTAGGGTGAATCGCCAATCTGTTCTGGGCGCTATCACATCTGCTCAGCAGAGGCTCAAGCTGTATAACAAGGTTCCCCCAAATGGCCTTGTGCTATATACTGGGACAATCGTGACTGAAGATGGAAAAGAAAAGAAAGTCACAATTGATTTTGAACCTTTCAGGCCTATAAATGCATCTCTCTATCTCTGTGACAACAAGTTCCATACGGAAGCCCTGAATGAACTTTTAGAATCTGATGACAAGTTTGGCTTCATTGTCATGGATGGTAATGGAACACTCTTTGGTACATTGAGTGGTAACACCCGCGAGGTCCTTCACAAATTTAGTGTTGACCTTCCTAAGAAACATGGGAGAGGAGGTCAATCAGCTCTTAGGTTTGCCCGTCTTCGGATGGAGAAGCGGCACAACTATGTGAGGAAAACAGCAGAGCTTGCAACCCAGTTCTTTATTAATCCTGCCACTAGTCAACCTAATGTTGCAGGATTGATACTGGCTGGATCAGCTGACTTCAAGACAGAGCTCAGTCAATCGGACATGTTTGATCCTCGTCTTCAAGCCAAAATACTTAATGTGGTTGATGTCTCTTATGGAGGTGAAAATGGATTCAATCAGGCCATTGAATTGTCGTCTGAGATCTTGTCTAATGTAAAATTTATACAGGAGAAGCGCTTGATTGGAAAATACTTTGAAGAGATTAGCCAGGACACAGGGAAATATGTTTTTGGTGTTGATGACACACTGAAAGCTCTGGAGATGGGTGCTGTCGAGATACTCATTGTTTGGGAAAATTTGGATATCAATAGGTACGTATTAAAGAATGCTGCCACTGGTGAGATTGCTATAAAGCACTTGAATAAGGAACAAGAAGCCAATCAGAGCAACTTCCGTGACCCTGTCAGCTCCGCCGAGTTGGAGGTTCAAGAAAAGATGGCCTTGCTGGAATGGTTTGCAAACGAGTACAAGCAGTTTGGTTGCACACTGGAGTTCGTTACCAACAAATCACAGGAAGGATCACAGTTCTGCAGAGGTTTTGGTGGTATTGGAGGAATTCTTCGTTACCAGCTTGACATAAGATCGTTTGATGAACTATCTGATGGGGAAGAGTACGGTGATTCTGAATAGCAATCAATCAATCACTCCCCGTTGCTGGTGCAAAAGGGGGATAAAGAGCTTGCACCAGCGCCCAGGGTTCGCGGAGGTTAGTCTTGGTTTGAACTGGAAAATTTGTGAAGGCTTTATTAAGGTTTATTTAGCCCCTCTCCACGCTGGTTCTTTTTGCTTTCACCAATTATTGTTTCAATTTACATATGTATTGCCTCACATGCTTCAATCTATCCCATGTACTTGTAGGGGTTTTGTCCTTTTGATCTGGATTTGGTGCCAATTATCTGATAGATCGTCAAGGATGCGCGCTCCAATTTGTAGCCACAGTGCTGCCCTTGTTCTCGTGGAATGGTTGCTGTCTGCAGCAGATACTCAGTTACAAGAATATTTGAAGTACTGCAAATAATATTCAATGCCGTATGATCTTAACTTTTTGAGTTGGTGTCATGTCTGACTATTATCGTTGCTGATAAACCATGTCAGTTGGATCTGAATTCATATGCATGTTACAGTATTTGAAGGTTGTTTTGTTGGGTATTGTTTTTGGATCTCAATTATATGTTTGGAATCAATAGAAAACGGCAAGATGAAGTAGGCTACAAAATAGTCCCCATAACAACTTTTTTGAAGATCATCTAAACAAACTCTAAACCGTTACTCTACTTGTTCTAAGCGTGTTTGGTATGCCCTGAGGTTCTCCGTAGATGATCAAGAGGGACTTTTTTAACTTTAACAGGTGGGGTGGAGATTTGGCCTTTTTTTTTAGTTGAGGATATAATAGCTTGATCAGTTGAGCTATGTTAGGTTGGTGATAAGTTGTTTTTGTTAAGTATTTAAAGTGTTTTTAGAGAGAGTATTTGACTAGTTTTCTTTCAGAAGTGCTTAAATAATAAGCTAATTGTGGCTGTGCTGTATGCCTTTATAAGCACCAAGTGCTTAGAGATGATGAAGAGTGCTTTTGTAAAGGTGTATTTAAAGTATTTAAGAG

mRNA sequence

CCCAGCCCGACCTCTTCCAGCCCAACAACTTTTCCCCCAACTCATGTAGAAACCCTAAATCTCACAAATTCAATCTCTCATTTTCAATCCTAGATCTCTCTCGTCTCTCCCGAGCAGAGTCGTTTGCGGCCCCTCTCCTCCGATTGCTTACCCAAGTTAGCAACTTGCCATCTTCAATCTCTCAGATCTCTTCCGGTGCTCCGAGATCTCTCTCGCTAATATCTTCCCGGCGGCATCTCTCACGAGCATCATCCTAGCGGCCGGCGGCTGCTCTCCCTCACGAACACCCATCGCGGCGGTATCCTTCTCTCTTGAGCACCCATCCCGCCGTACTCTCATTTATTACGATGGCTGACGGTCATGAAACTGATAAGAACATTGAAATATGGAAAATTAAGAAGTTGATTAAAGCACTTGAAGCTGCTAGGGGAAATGGGACTAGCATGATTTCTCTCATTATGCCTCCTCGTGATCAAATATCTCGTGTTACTAAGATGCTTGGTGATGAATTTGGAACTGCTTCGAACATTAAGAGTAGGGTGAATCGCCAATCTGTTCTGGGCGCTATCACATCTGCTCAGCAGAGGCTCAAGCTGTATAACAAGGTTCCCCCAAATGGCCTTGTGCTATATACTGGGACAATCGTGACTGAAGATGGAAAAGAAAAGAAAGTCACAATTGATTTTGAACCTTTCAGGCCTATAAATGCATCTCTCTATCTCTGTGACAACAAGTTCCATACGGAAGCCCTGAATGAACTTTTAGAATCTGATGACAAGTTTGGCTTCATTGTCATGGATGGTAATGGAACACTCTTTGGTACATTGAGTGGTAACACCCGCGAGGTCCTTCACAAATTTAGTGTTGACCTTCCTAAGAAACATGGGAGAGGAGGTCAATCAGCTCTTAGGTTTGCCCGTCTTCGGATGGAGAAGCGGCACAACTATGTGAGGAAAACAGCAGAGCTTGCAACCCAGTTCTTTATTAATCCTGCCACTAGTCAACCTAATGTTGCAGGATTGATACTGGCTGGATCAGCTGACTTCAAGACAGAGCTCAGTCAATCGGACATGTTTGATCCTCGTCTTCAAGCCAAAATACTTAATGTGGTTGATGTCTCTTATGGAGGTGAAAATGGATTCAATCAGGCCATTGAATTGTCGTCTGAGATCTTGTCTAATGTAAAATTTATACAGGAGAAGCGCTTGATTGGAAAATACTTTGAAGAGATTAGCCAGGACACAGGGAAATATGTTTTTGGTGTTGATGACACACTGAAAGCTCTGGAGATGGGTGCTGTCGAGATACTCATTGTTTGGGAAAATTTGGATATCAATAGGTACGTATTAAAGAATGCTGCCACTGGTGAGATTGCTATAAAGCACTTGAATAAGGAACAAGAAGCCAATCAGAGCAACTTCCGTGACCCTGTCAGCTCCGCCGAGTTGGAGGTTCAAGAAAAGATGGCCTTGCTGGAATGGTTTGCAAACGAGTACAAGCAGTTTGGTTGCACACTGGAGTTCGTTACCAACAAATCACAGGAAGGATCACAGTTCTGCAGAGGTTTTGGTGGTATTGGAGGAATTCTTCGTTACCAGCTTGACATAAGATCGTTTGATGAACTATCTGATGGGGAAGAGTACGGTGATTCTGAATAGCAATCAATCAATCACTCCCCGTTGCTGGTGCAAAAGGGGGATAAAGAGCTTGCACCAGCGCCCAGGGTTCGCGGAGGTTAGTCTTGGTTTGAACTGGAAAATTTGTGAAGGCTTTATTAAGGGGTTTTGTCCTTTTGATCTGGATTTGGTGCCAATTATCTGATAGATCGTCAAGGATGCGCGCTCCAATTTGTAGCCACAGTGCTGCCCTTGTTCTCGTGGAATGGTTGCTGTCTGCAGCAGATACTCAGTTACAAGAATATTTGAAGTACTGCAAATAATATTCAATGCCGTATGATCTTAACTTTTTGAGTTGGTGTCATGTCTGACTATTATCGTTGCTGATAAACCATGTCAGTTGGATCTGAATTCATATGCATGTTACAGTATTTGAAGGTTGTTTTGTTGGGTATTGTTTTTGGATCTCAATTATATGTTTGGAATCAATAGAAAACGGCAAGATGAAGTAGGCTACAAAATAGTCCCCATAACAACTTTTTTGAAGATCATCTAAACAAACTCTAAACCGTTACTCTACTTGTTCTAAGCGTGTTTGGTATGCCCTGAGGTTCTCCGTAGATGATCAAGAGGGACTTTTTTAACTTTAACAGGTGGGGTGGAGATTTGGCCTTTTTTTTTAGTTGAGGATATAATAGCTTGATCAGTTGAGCTATGTTAGGTTGGTGATAAGTTGTTTTTGTTAAGTATTTAAAGTGTTTTTAGAGAGAGTATTTGACTAGTTTTCTTTCAGAAGTGCTTAAATAATAAGCTAATTGTGGCTGTGCTGTATGCCTTTATAAGCACCAAGTGCTTAGAGATGATGAAGAGTGCTTTTGTAAAGGTGTATTTAAAGTATTTAAGAG

Coding sequence (CDS)

ATGGCTGACGGTCATGAAACTGATAAGAACATTGAAATATGGAAAATTAAGAAGTTGATTAAAGCACTTGAAGCTGCTAGGGGAAATGGGACTAGCATGATTTCTCTCATTATGCCTCCTCGTGATCAAATATCTCGTGTTACTAAGATGCTTGGTGATGAATTTGGAACTGCTTCGAACATTAAGAGTAGGGTGAATCGCCAATCTGTTCTGGGCGCTATCACATCTGCTCAGCAGAGGCTCAAGCTGTATAACAAGGTTCCCCCAAATGGCCTTGTGCTATATACTGGGACAATCGTGACTGAAGATGGAAAAGAAAAGAAAGTCACAATTGATTTTGAACCTTTCAGGCCTATAAATGCATCTCTCTATCTCTGTGACAACAAGTTCCATACGGAAGCCCTGAATGAACTTTTAGAATCTGATGACAAGTTTGGCTTCATTGTCATGGATGGTAATGGAACACTCTTTGGTACATTGAGTGGTAACACCCGCGAGGTCCTTCACAAATTTAGTGTTGACCTTCCTAAGAAACATGGGAGAGGAGGTCAATCAGCTCTTAGGTTTGCCCGTCTTCGGATGGAGAAGCGGCACAACTATGTGAGGAAAACAGCAGAGCTTGCAACCCAGTTCTTTATTAATCCTGCCACTAGTCAACCTAATGTTGCAGGATTGATACTGGCTGGATCAGCTGACTTCAAGACAGAGCTCAGTCAATCGGACATGTTTGATCCTCGTCTTCAAGCCAAAATACTTAATGTGGTTGATGTCTCTTATGGAGGTGAAAATGGATTCAATCAGGCCATTGAATTGTCGTCTGAGATCTTGTCTAATGTAAAATTTATACAGGAGAAGCGCTTGATTGGAAAATACTTTGAAGAGATTAGCCAGGACACAGGGAAATATGTTTTTGGTGTTGATGACACACTGAAAGCTCTGGAGATGGGTGCTGTCGAGATACTCATTGTTTGGGAAAATTTGGATATCAATAGGTACGTATTAAAGAATGCTGCCACTGGTGAGATTGCTATAAAGCACTTGAATAAGGAACAAGAAGCCAATCAGAGCAACTTCCGTGACCCTGTCAGCTCCGCCGAGTTGGAGGTTCAAGAAAAGATGGCCTTGCTGGAATGGTTTGCAAACGAGTACAAGCAGTTTGGTTGCACACTGGAGTTCGTTACCAACAAATCACAGGAAGGATCACAGTTCTGCAGAGGTTTTGGTGGTATTGGAGGAATTCTTCGTTACCAGCTTGACATAAGATCGTTTGATGAACTATCTGATGGGGAAGAGTACGGTGATTCTGAATAG

Protein sequence

MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASNIKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPINASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHGRGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQSDMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTGKYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNAATGEIAIKHLNKEQEANQSNFRDPVSSAELEVQEKMALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDIRSFDELSDGEEYGDSE
Homology
BLAST of Tan0004530 vs. ExPASy Swiss-Prot
Match: P35614 (Eukaryotic peptide chain release factor subunit 1-3 OS=Arabidopsis thaliana OX=3702 GN=ERF1-3 PE=1 SV=1)

HSP 1 Score: 793.1 bits (2047), Expect = 1.6e-228
Identity = 394/436 (90.37%), Postives = 416/436 (95.41%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MAD  E+DKNIEIWKIKKLIK LE ARGNGTSMISLIMPPRDQ++RVTKML DE+GTASN
Sbjct: 1   MAD-QESDKNIEIWKIKKLIKGLETARGNGTSMISLIMPPRDQVARVTKMLADEYGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVL AITSAQQRLKLYNKVPPNGLVLYTGTIVT+DGKEKKVTIDFEPF+PIN
Sbjct: 61  IKSRVNRQSVLSAITSAQQRLKLYNKVPPNGLVLYTGTIVTDDGKEKKVTIDFEPFKPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTE LNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKF+VDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEPLNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFTVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQF+INPATSQPNV+GLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFYINPATSQPNVSGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           ++FDPRLQAKILNVVDVSYGGENGFNQAIELS+EILSNVKFIQEK+LIGKYFEEISQDTG
Sbjct: 241 ELFDPRLQAKILNVVDVSYGGENGFNQAIELSAEILSNVKFIQEKKLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNAATGEIAIKHLNKEQEANQSNFRD 360
           KYVFGV+DTLKALEMGAVE LIVWENLDINRY LKN  TGEI IKHL K+QE NQSNF D
Sbjct: 301 KYVFGVEDTLKALEMGAVETLIVWENLDINRYELKNNTTGEIVIKHLGKDQENNQSNFHD 360

Query: 361 PVSSAELEVQEKMALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420
             ++AELEVQEKM LLEWFANEYK+FGCTLEFVTNKSQEGSQFCRGFGGIGG+LRYQLD+
Sbjct: 361 AETNAELEVQEKMPLLEWFANEYKRFGCTLEFVTNKSQEGSQFCRGFGGIGGLLRYQLDM 420

Query: 421 RSFDELSDGEEYGDSE 437
           R+FDELSDGE Y DS+
Sbjct: 421 RTFDELSDGEVYEDSD 435

BLAST of Tan0004530 vs. ExPASy Swiss-Prot
Match: D2K759 (Eukaryotic peptide chain release factor subunit 1-2 OS=Brassica oleracea var. botrytis OX=3715 GN=ERF1-2 PE=1 SV=1)

HSP 1 Score: 785.0 bits (2026), Expect = 4.3e-226
Identity = 389/436 (89.22%), Postives = 413/436 (94.72%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MAD  E D NIEIWKIKKLIK LE+ARGNGTSMISLIMPPRDQ+SRVTKMLGDE+GTASN
Sbjct: 1   MAD-QEADTNIEIWKIKKLIKGLESARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVL AITSAQQRLKLYNKVP NGLVLYTGTIV +DGKEKKVT DFEPFRPIN
Sbjct: 61  IKSRVNRQSVLSAITSAQQRLKLYNKVPTNGLVLYTGTIVNDDGKEKKVTFDFEPFRPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHT ALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKF+VDLPKKHG
Sbjct: 121 ASLYLCDNKFHTGALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFTVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQF+INPATSQPNVAGLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFYINPATSQPNVAGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           ++FDPRLQAKILNVVDVSYGGENGFNQAIELS+EILSNVKFIQEK+LIGKYFEEISQDTG
Sbjct: 241 ELFDPRLQAKILNVVDVSYGGENGFNQAIELSAEILSNVKFIQEKKLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNAATGEIAIKHLNKEQEANQSNFRD 360
           KYVFGV+DTLKALEMGA+E LIVWENLDINRY LKN+ +GEI +KH  K+QE +QSNF D
Sbjct: 301 KYVFGVEDTLKALEMGAIETLIVWENLDINRYELKNSTSGEIVVKHFGKDQETDQSNFHD 360

Query: 361 PVSSAELEVQEKMALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420
             ++AELEVQEKM LLEWFANEYK+FGCTLEFVTNKSQEGSQFCRGFGGIGG+LRYQLD+
Sbjct: 361 AETNAELEVQEKMPLLEWFANEYKRFGCTLEFVTNKSQEGSQFCRGFGGIGGMLRYQLDM 420

Query: 421 RSFDELSDGEEYGDSE 437
           R+FDELSDGE Y DS+
Sbjct: 421 RTFDELSDGEVYEDSD 435

BLAST of Tan0004530 vs. ExPASy Swiss-Prot
Match: D2K760 (Eukaryotic peptide chain release factor subunit 1-3 OS=Brassica oleracea var. botrytis OX=3715 GN=ERF1-3 PE=2 SV=1)

HSP 1 Score: 784.3 bits (2024), Expect = 7.3e-226
Identity = 390/436 (89.45%), Postives = 414/436 (94.95%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MAD  E+DK+IEIWK  KLIK LE+ARGNGTSMISLIMPPRDQ++RVTKML DE+GTASN
Sbjct: 1   MAD-QESDKSIEIWKSFKLIKGLESARGNGTSMISLIMPPRDQVARVTKMLADEYGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVL AITSAQQRLKLYNKVPPNGLVLYTGTIVT+DGKEKKVTIDFEPF+PIN
Sbjct: 61  IKSRVNRQSVLSAITSAQQRLKLYNKVPPNGLVLYTGTIVTDDGKEKKVTIDFEPFKPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTE LNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKF+VDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEPLNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFTVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQF+INPATSQPNV+GLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFYINPATSQPNVSGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           ++FDPRLQAKILNVVDVSYGGENGFNQAIELS+EILSNVKFIQEK+LIGKYFEEISQDTG
Sbjct: 241 ELFDPRLQAKILNVVDVSYGGENGFNQAIELSAEILSNVKFIQEKKLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNAATGEIAIKHLNKEQEANQSNFRD 360
           KYVFGVDDTLKAL+MGAVE LIVWENLDINRY LKN ATGE  IKHL KEQE +QSNF D
Sbjct: 301 KYVFGVDDTLKALDMGAVETLIVWENLDINRYELKNGATGETVIKHLGKEQENDQSNFHD 360

Query: 361 PVSSAELEVQEKMALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420
             S+AELE+ EKM LLEWFANEYK+FGCTLEFVTNKSQEGSQFCRGFGGIGG+LRYQLD+
Sbjct: 361 AESNAELEIVEKMPLLEWFANEYKRFGCTLEFVTNKSQEGSQFCRGFGGIGGLLRYQLDM 420

Query: 421 RSFDELSDGEEYGDSE 437
           R+FDELSDGE Y DS+
Sbjct: 421 RTFDELSDGEVYEDSD 435

BLAST of Tan0004530 vs. ExPASy Swiss-Prot
Match: Q9LPV8 (Eukaryotic peptide chain release factor subunit 1-2 OS=Arabidopsis thaliana OX=3702 GN=ERF1-2 PE=1 SV=1)

HSP 1 Score: 780.4 bits (2014), Expect = 1.1e-224
Identity = 384/431 (89.10%), Postives = 410/431 (95.13%), Query Frame = 0

Query: 6   ETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASNIKSRV 65
           E D NIEIWKIKKLIK LE+ARGNGTSMISLIMPPRDQ+SRVTKMLGDE+GTASNIKSRV
Sbjct: 4   EADTNIEIWKIKKLIKGLESARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASNIKSRV 63

Query: 66  NRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPINASLYL 125
           NRQSVL AITSAQQRLKLYNKVP NGLVLYTGTIV +DGKEKKVT DFEPFRPINASLYL
Sbjct: 64  NRQSVLSAITSAQQRLKLYNKVPTNGLVLYTGTIVNDDGKEKKVTFDFEPFRPINASLYL 123

Query: 126 CDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHGRGGQS 185
           CDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKF+VDLPKKHGRGGQS
Sbjct: 124 CDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFTVDLPKKHGRGGQS 183

Query: 186 ALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQSDMFDP 245
           ALRFARLRMEKRHNYVRKTAELATQF+INPATSQPNV+GLILAGSADFKTELSQS++FDP
Sbjct: 184 ALRFARLRMEKRHNYVRKTAELATQFYINPATSQPNVSGLILAGSADFKTELSQSELFDP 243

Query: 246 RLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTGKYVFG 305
           RLQAKILNVVDVSYGGENGFNQAIELS+EILSNVKFIQEK+LIGKYFEEISQDTGKYVFG
Sbjct: 244 RLQAKILNVVDVSYGGENGFNQAIELSAEILSNVKFIQEKKLIGKYFEEISQDTGKYVFG 303

Query: 306 VDDTLKALEMGAVEILIVWENLDINRYVLKNAATGEIAIKHLNKEQEANQSNFRDPVSSA 365
           V+DTLKALEMGA+E LIVWENLDINRY LKN+ TGE+ +KH  K+QE++ SNF D  ++A
Sbjct: 304 VEDTLKALEMGAIETLIVWENLDINRYELKNSTTGEMVVKHFGKDQESDTSNFHDSETNA 363

Query: 366 ELEVQEKMALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDIRSFDE 425
           ELEVQEKM LLEWFANEYK+FGCTLEFVTNKSQEGSQFCRGFGGIGG+LRYQLD+R+FDE
Sbjct: 364 ELEVQEKMPLLEWFANEYKRFGCTLEFVTNKSQEGSQFCRGFGGIGGMLRYQLDMRTFDE 423

Query: 426 LSDGEEYGDSE 437
           LSD E Y DS+
Sbjct: 424 LSDTEVYEDSD 434

BLAST of Tan0004530 vs. ExPASy Swiss-Prot
Match: Q39097 (Eukaryotic peptide chain release factor subunit 1-1 OS=Arabidopsis thaliana OX=3702 GN=ERF1-1 PE=1 SV=2)

HSP 1 Score: 772.7 bits (1994), Expect = 2.2e-222
Identity = 383/434 (88.25%), Postives = 407/434 (93.78%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           M D ++ DKNIEIWKIKKLIK+LEAARGNGTSMISLIMPPRDQ+SRVTKMLGDE+GTASN
Sbjct: 1   MGDKNDDDKNIEIWKIKKLIKSLEAARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVLGAITSAQQRLKLYN+VPPNGLVLYTGTIV EDGKEKKVTIDFEPFRPIN
Sbjct: 61  IKSRVNRQSVLGAITSAQQRLKLYNRVPPNGLVLYTGTIVNEDGKEKKVTIDFEPFRPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQ++INPATSQPNV+GLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQYYINPATSQPNVSGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           DMFDPRL AKILNVVDVSYGGENGFNQAIELS+EIL+NVKFIQEKRLIGKYFEEISQDTG
Sbjct: 241 DMFDPRLAAKILNVVDVSYGGENGFNQAIELSAEILANVKFIQEKRLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNAATGEIAIKHLNKEQEANQSNFRD 360
           KYVFGV+DTL ALE GA+E LIVWENLDINRYV+KN+ATGE  IKHLNKEQEAN  NF+ 
Sbjct: 301 KYVFGVEDTLNALESGAIETLIVWENLDINRYVMKNSATGETVIKHLNKEQEANTENFKV 360

Query: 361 PVSSAELEVQEKMALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420
             S   L+V+EK++LLEW ANEY++FGC LEFVTNKSQEGSQFCRGFGGIGGILRYQLD+
Sbjct: 361 ADSDLALDVEEKLSLLEWLANEYRRFGCALEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420

Query: 421 RSFDELSDGEEYGD 435
            +FD   DGE   D
Sbjct: 421 TAFDS-EDGEALDD 433

BLAST of Tan0004530 vs. NCBI nr
Match: XP_022148872.1 (eukaryotic peptide chain release factor subunit 1-3-like [Momordica charantia])

HSP 1 Score: 862.1 bits (2226), Expect = 2.1e-246
Identity = 432/436 (99.08%), Postives = 435/436 (99.77%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN
Sbjct: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN
Sbjct: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNV+GLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVSGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG
Sbjct: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNAATGEIAIKHLNKEQEANQSNFRD 360
           KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNAATGEI IKHLNKEQEA+QSNFRD
Sbjct: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNAATGEIIIKHLNKEQEASQSNFRD 360

Query: 361 PVSSAELEVQEKMALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420
           PVSSAELEVQEKMALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI
Sbjct: 361 PVSSAELEVQEKMALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420

Query: 421 RSFDELSDGEEYGDSE 437
           RSFDELSDGE+YGDSE
Sbjct: 421 RSFDELSDGEDYGDSE 436

BLAST of Tan0004530 vs. NCBI nr
Match: KAG7014612.1 (Eukaryotic peptide chain release factor subunit 1-3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 858.2 bits (2216), Expect = 3.0e-245
Identity = 428/436 (98.17%), Postives = 434/436 (99.54%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN
Sbjct: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN
Sbjct: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQF+INPATSQPNVAGLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFYINPATSQPNVAGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG
Sbjct: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNAATGEIAIKHLNKEQEANQSNFRD 360
           KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKN +TGE+ IKHLNKEQEANQSNFRD
Sbjct: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNVSTGEVIIKHLNKEQEANQSNFRD 360

Query: 361 PVSSAELEVQEKMALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420
           PV++AELEVQEKMALLEWFANEYK+FGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI
Sbjct: 361 PVTAAELEVQEKMALLEWFANEYKKFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420

Query: 421 RSFDELSDGEEYGDSE 437
           RSFDELSDGEEYGDSE
Sbjct: 421 RSFDELSDGEEYGDSE 436

BLAST of Tan0004530 vs. NCBI nr
Match: KAG6576095.1 (Eukaryotic peptide chain release factor subunit 1-3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 858.2 bits (2216), Expect = 3.0e-245
Identity = 428/436 (98.17%), Postives = 434/436 (99.54%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN
Sbjct: 106 MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 165

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN
Sbjct: 166 IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 225

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG
Sbjct: 226 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 285

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQF+INPATSQPNVAGLILAGSADFKTELSQS
Sbjct: 286 RGGQSALRFARLRMEKRHNYVRKTAELATQFYINPATSQPNVAGLILAGSADFKTELSQS 345

Query: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG
Sbjct: 346 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 405

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNAATGEIAIKHLNKEQEANQSNFRD 360
           KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKN +TGE+ IKHLNKEQEANQSNFRD
Sbjct: 406 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNVSTGEVIIKHLNKEQEANQSNFRD 465

Query: 361 PVSSAELEVQEKMALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420
           PV++AELEVQEKMALLEWFANEYK+FGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI
Sbjct: 466 PVTAAELEVQEKMALLEWFANEYKKFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 525

Query: 421 RSFDELSDGEEYGDSE 437
           RSFDELSDGEEYGDSE
Sbjct: 526 RSFDELSDGEEYGDSE 541

BLAST of Tan0004530 vs. NCBI nr
Match: XP_023548291.1 (eukaryotic peptide chain release factor subunit 1-3 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 857.8 bits (2215), Expect = 4.0e-245
Identity = 427/436 (97.94%), Postives = 434/436 (99.54%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN
Sbjct: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN
Sbjct: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQF+INPATSQPNVAGLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFYINPATSQPNVAGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG
Sbjct: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNAATGEIAIKHLNKEQEANQSNFRD 360
           KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKN +TGE+ IKHLNKEQEANQSNFRD
Sbjct: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNVSTGEVIIKHLNKEQEANQSNFRD 360

Query: 361 PVSSAELEVQEKMALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420
           P+++AELEVQEKMALLEWFANEYK+FGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI
Sbjct: 361 PITAAELEVQEKMALLEWFANEYKKFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420

Query: 421 RSFDELSDGEEYGDSE 437
           RSFDELSDGEEYGDSE
Sbjct: 421 RSFDELSDGEEYGDSE 436

BLAST of Tan0004530 vs. NCBI nr
Match: XP_022953222.1 (eukaryotic peptide chain release factor subunit 1-3 [Cucurbita moschata])

HSP 1 Score: 856.7 bits (2212), Expect = 8.9e-245
Identity = 427/436 (97.94%), Postives = 434/436 (99.54%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN
Sbjct: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN
Sbjct: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQF+INPATSQPNVAGLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFYINPATSQPNVAGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG
Sbjct: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNAATGEIAIKHLNKEQEANQSNFRD 360
           KYVFGV+DTLKALEMGAVEILIVWENLDINRYVLKN +TGE+ IKHLNKEQEANQSNFRD
Sbjct: 301 KYVFGVEDTLKALEMGAVEILIVWENLDINRYVLKNVSTGEVIIKHLNKEQEANQSNFRD 360

Query: 361 PVSSAELEVQEKMALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420
           PV++AELEVQEKMALLEWFANEYK+FGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI
Sbjct: 361 PVTAAELEVQEKMALLEWFANEYKKFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420

Query: 421 RSFDELSDGEEYGDSE 437
           RSFDELSDGEEYGDSE
Sbjct: 421 RSFDELSDGEEYGDSE 436

BLAST of Tan0004530 vs. ExPASy TrEMBL
Match: A0A6J1D677 (eukaryotic peptide chain release factor subunit 1-3-like OS=Momordica charantia OX=3673 GN=LOC111017439 PE=3 SV=1)

HSP 1 Score: 862.1 bits (2226), Expect = 1.0e-246
Identity = 432/436 (99.08%), Postives = 435/436 (99.77%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN
Sbjct: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN
Sbjct: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNV+GLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVSGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG
Sbjct: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNAATGEIAIKHLNKEQEANQSNFRD 360
           KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNAATGEI IKHLNKEQEA+QSNFRD
Sbjct: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNAATGEIIIKHLNKEQEASQSNFRD 360

Query: 361 PVSSAELEVQEKMALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420
           PVSSAELEVQEKMALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI
Sbjct: 361 PVSSAELEVQEKMALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420

Query: 421 RSFDELSDGEEYGDSE 437
           RSFDELSDGE+YGDSE
Sbjct: 421 RSFDELSDGEDYGDSE 436

BLAST of Tan0004530 vs. ExPASy TrEMBL
Match: A0A6J1GMN2 (eukaryotic peptide chain release factor subunit 1-3 OS=Cucurbita moschata OX=3662 GN=LOC111455832 PE=3 SV=1)

HSP 1 Score: 856.7 bits (2212), Expect = 4.3e-245
Identity = 427/436 (97.94%), Postives = 434/436 (99.54%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN
Sbjct: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN
Sbjct: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQF+INPATSQPNVAGLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFYINPATSQPNVAGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG
Sbjct: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNAATGEIAIKHLNKEQEANQSNFRD 360
           KYVFGV+DTLKALEMGAVEILIVWENLDINRYVLKN +TGE+ IKHLNKEQEANQSNFRD
Sbjct: 301 KYVFGVEDTLKALEMGAVEILIVWENLDINRYVLKNVSTGEVIIKHLNKEQEANQSNFRD 360

Query: 361 PVSSAELEVQEKMALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420
           PV++AELEVQEKMALLEWFANEYK+FGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI
Sbjct: 361 PVTAAELEVQEKMALLEWFANEYKKFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420

Query: 421 RSFDELSDGEEYGDSE 437
           RSFDELSDGEEYGDSE
Sbjct: 421 RSFDELSDGEEYGDSE 436

BLAST of Tan0004530 vs. ExPASy TrEMBL
Match: A0A6J1H946 (eukaryotic peptide chain release factor subunit 1-3-like OS=Cucurbita moschata OX=3662 GN=LOC111461184 PE=3 SV=1)

HSP 1 Score: 855.1 bits (2208), Expect = 1.2e-244
Identity = 428/436 (98.17%), Postives = 433/436 (99.31%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN
Sbjct: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVLGAITSAQQRLKLY+KVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN
Sbjct: 61  IKSRVNRQSVLGAITSAQQRLKLYSKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           DMFDPRLQ KILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG
Sbjct: 241 DMFDPRLQTKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNAATGEIAIKHLNKEQEANQSNFRD 360
           KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNA+TGEI IKHLNKEQEANQSNFRD
Sbjct: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIVIKHLNKEQEANQSNFRD 360

Query: 361 PVSSAELEVQEKMALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420
            V+SAELEVQEK+ALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLD+
Sbjct: 361 SVTSAELEVQEKVALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420

Query: 421 RSFDELSDGEEYGDSE 437
           RSFDELSDGEEYGDSE
Sbjct: 421 RSFDELSDGEEYGDSE 436

BLAST of Tan0004530 vs. ExPASy TrEMBL
Match: A0A0A0KF54 (eRF1_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G089230 PE=3 SV=1)

HSP 1 Score: 854.7 bits (2207), Expect = 1.6e-244
Identity = 428/436 (98.17%), Postives = 432/436 (99.08%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MAD HETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN
Sbjct: 1   MADAHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN
Sbjct: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG
Sbjct: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNAATGEIAIKHLNKEQEANQSNFRD 360
           KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNA+TGEI IKHLNKEQEANQSNFRD
Sbjct: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNASTGEIIIKHLNKEQEANQSNFRD 360

Query: 361 PVSSAELEVQEKMALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420
           P +S+ELEVQEKMALLEWFANEYK+FGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI
Sbjct: 361 PATSSELEVQEKMALLEWFANEYKKFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420

Query: 421 RSFDELSDGEEYGDSE 437
           RSFDELSDGEEY DSE
Sbjct: 421 RSFDELSDGEEYDDSE 436

BLAST of Tan0004530 vs. ExPASy TrEMBL
Match: A0A6J1JV14 (eukaryotic peptide chain release factor subunit 1-3 OS=Cucurbita maxima OX=3661 GN=LOC111488098 PE=3 SV=1)

HSP 1 Score: 854.0 bits (2205), Expect = 2.8e-244
Identity = 426/436 (97.71%), Postives = 433/436 (99.31%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN
Sbjct: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN
Sbjct: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQF+INPATSQPNVAGLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFYINPATSQPNVAGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG
Sbjct: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNAATGEIAIKHLNKEQEANQSNFRD 360
           KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKN +TGE+ IKHLNKEQEANQSNFRD
Sbjct: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNVSTGEVIIKHLNKEQEANQSNFRD 360

Query: 361 PVSSAELEVQEKMALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420
            +++AELEVQEKMALLEWFANEYK+FGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI
Sbjct: 361 LITAAELEVQEKMALLEWFANEYKKFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420

Query: 421 RSFDELSDGEEYGDSE 437
           RSFDELSDGEEYGDSE
Sbjct: 421 RSFDELSDGEEYGDSE 436

BLAST of Tan0004530 vs. TAIR 10
Match: AT3G26618.1 (eukaryotic release factor 1-3 )

HSP 1 Score: 793.1 bits (2047), Expect = 1.1e-229
Identity = 394/436 (90.37%), Postives = 416/436 (95.41%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           MAD  E+DKNIEIWKIKKLIK LE ARGNGTSMISLIMPPRDQ++RVTKML DE+GTASN
Sbjct: 1   MAD-QESDKNIEIWKIKKLIKGLETARGNGTSMISLIMPPRDQVARVTKMLADEYGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVL AITSAQQRLKLYNKVPPNGLVLYTGTIVT+DGKEKKVTIDFEPF+PIN
Sbjct: 61  IKSRVNRQSVLSAITSAQQRLKLYNKVPPNGLVLYTGTIVTDDGKEKKVTIDFEPFKPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTE LNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKF+VDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEPLNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFTVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQF+INPATSQPNV+GLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFYINPATSQPNVSGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           ++FDPRLQAKILNVVDVSYGGENGFNQAIELS+EILSNVKFIQEK+LIGKYFEEISQDTG
Sbjct: 241 ELFDPRLQAKILNVVDVSYGGENGFNQAIELSAEILSNVKFIQEKKLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNAATGEIAIKHLNKEQEANQSNFRD 360
           KYVFGV+DTLKALEMGAVE LIVWENLDINRY LKN  TGEI IKHL K+QE NQSNF D
Sbjct: 301 KYVFGVEDTLKALEMGAVETLIVWENLDINRYELKNNTTGEIVIKHLGKDQENNQSNFHD 360

Query: 361 PVSSAELEVQEKMALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420
             ++AELEVQEKM LLEWFANEYK+FGCTLEFVTNKSQEGSQFCRGFGGIGG+LRYQLD+
Sbjct: 361 AETNAELEVQEKMPLLEWFANEYKRFGCTLEFVTNKSQEGSQFCRGFGGIGGLLRYQLDM 420

Query: 421 RSFDELSDGEEYGDSE 437
           R+FDELSDGE Y DS+
Sbjct: 421 RTFDELSDGEVYEDSD 435

BLAST of Tan0004530 vs. TAIR 10
Match: AT1G12920.1 (eukaryotic release factor 1-2 )

HSP 1 Score: 780.4 bits (2014), Expect = 7.5e-226
Identity = 384/431 (89.10%), Postives = 410/431 (95.13%), Query Frame = 0

Query: 6   ETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASNIKSRV 65
           E D NIEIWKIKKLIK LE+ARGNGTSMISLIMPPRDQ+SRVTKMLGDE+GTASNIKSRV
Sbjct: 4   EADTNIEIWKIKKLIKGLESARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASNIKSRV 63

Query: 66  NRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPINASLYL 125
           NRQSVL AITSAQQRLKLYNKVP NGLVLYTGTIV +DGKEKKVT DFEPFRPINASLYL
Sbjct: 64  NRQSVLSAITSAQQRLKLYNKVPTNGLVLYTGTIVNDDGKEKKVTFDFEPFRPINASLYL 123

Query: 126 CDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHGRGGQS 185
           CDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKF+VDLPKKHGRGGQS
Sbjct: 124 CDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFTVDLPKKHGRGGQS 183

Query: 186 ALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQSDMFDP 245
           ALRFARLRMEKRHNYVRKTAELATQF+INPATSQPNV+GLILAGSADFKTELSQS++FDP
Sbjct: 184 ALRFARLRMEKRHNYVRKTAELATQFYINPATSQPNVSGLILAGSADFKTELSQSELFDP 243

Query: 246 RLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTGKYVFG 305
           RLQAKILNVVDVSYGGENGFNQAIELS+EILSNVKFIQEK+LIGKYFEEISQDTGKYVFG
Sbjct: 244 RLQAKILNVVDVSYGGENGFNQAIELSAEILSNVKFIQEKKLIGKYFEEISQDTGKYVFG 303

Query: 306 VDDTLKALEMGAVEILIVWENLDINRYVLKNAATGEIAIKHLNKEQEANQSNFRDPVSSA 365
           V+DTLKALEMGA+E LIVWENLDINRY LKN+ TGE+ +KH  K+QE++ SNF D  ++A
Sbjct: 304 VEDTLKALEMGAIETLIVWENLDINRYELKNSTTGEMVVKHFGKDQESDTSNFHDSETNA 363

Query: 366 ELEVQEKMALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDIRSFDE 425
           ELEVQEKM LLEWFANEYK+FGCTLEFVTNKSQEGSQFCRGFGGIGG+LRYQLD+R+FDE
Sbjct: 364 ELEVQEKMPLLEWFANEYKRFGCTLEFVTNKSQEGSQFCRGFGGIGGMLRYQLDMRTFDE 423

Query: 426 LSDGEEYGDSE 437
           LSD E Y DS+
Sbjct: 424 LSDTEVYEDSD 434

BLAST of Tan0004530 vs. TAIR 10
Match: AT5G47880.1 (eukaryotic release factor 1-1 )

HSP 1 Score: 772.7 bits (1994), Expect = 1.6e-223
Identity = 383/434 (88.25%), Postives = 407/434 (93.78%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           M D ++ DKNIEIWKIKKLIK+LEAARGNGTSMISLIMPPRDQ+SRVTKMLGDE+GTASN
Sbjct: 1   MGDKNDDDKNIEIWKIKKLIKSLEAARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVLGAITSAQQRLKLYN+VPPNGLVLYTGTIV EDGKEKKVTIDFEPFRPIN
Sbjct: 61  IKSRVNRQSVLGAITSAQQRLKLYNRVPPNGLVLYTGTIVNEDGKEKKVTIDFEPFRPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQ++INPATSQPNV+GLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQYYINPATSQPNVSGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           DMFDPRL AKILNVVDVSYGGENGFNQAIELS+EIL+NVKFIQEKRLIGKYFEEISQDTG
Sbjct: 241 DMFDPRLAAKILNVVDVSYGGENGFNQAIELSAEILANVKFIQEKRLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNAATGEIAIKHLNKEQEANQSNFRD 360
           KYVFGV+DTL ALE GA+E LIVWENLDINRYV+KN+ATGE  IKHLNKEQEAN  NF+ 
Sbjct: 301 KYVFGVEDTLNALESGAIETLIVWENLDINRYVMKNSATGETVIKHLNKEQEANTENFKV 360

Query: 361 PVSSAELEVQEKMALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420
             S   L+V+EK++LLEW ANEY++FGC LEFVTNKSQEGSQFCRGFGGIGGILRYQLD+
Sbjct: 361 ADSDLALDVEEKLSLLEWLANEYRRFGCALEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420

Query: 421 RSFDELSDGEEYGD 435
            +FD   DGE   D
Sbjct: 421 TAFDS-EDGEALDD 433

BLAST of Tan0004530 vs. TAIR 10
Match: AT5G47880.2 (eukaryotic release factor 1-1 )

HSP 1 Score: 772.7 bits (1994), Expect = 1.6e-223
Identity = 383/434 (88.25%), Postives = 407/434 (93.78%), Query Frame = 0

Query: 1   MADGHETDKNIEIWKIKKLIKALEAARGNGTSMISLIMPPRDQISRVTKMLGDEFGTASN 60
           M D ++ DKNIEIWKIKKLIK+LEAARGNGTSMISLIMPPRDQ+SRVTKMLGDE+GTASN
Sbjct: 1   MGDKNDDDKNIEIWKIKKLIKSLEAARGNGTSMISLIMPPRDQVSRVTKMLGDEYGTASN 60

Query: 61  IKSRVNRQSVLGAITSAQQRLKLYNKVPPNGLVLYTGTIVTEDGKEKKVTIDFEPFRPIN 120
           IKSRVNRQSVLGAITSAQQRLKLYN+VPPNGLVLYTGTIV EDGKEKKVTIDFEPFRPIN
Sbjct: 61  IKSRVNRQSVLGAITSAQQRLKLYNRVPPNGLVLYTGTIVNEDGKEKKVTIDFEPFRPIN 120

Query: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180
           ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG
Sbjct: 121 ASLYLCDNKFHTEALNELLESDDKFGFIVMDGNGTLFGTLSGNTREVLHKFSVDLPKKHG 180

Query: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQFFINPATSQPNVAGLILAGSADFKTELSQS 240
           RGGQSALRFARLRMEKRHNYVRKTAELATQ++INPATSQPNV+GLILAGSADFKTELSQS
Sbjct: 181 RGGQSALRFARLRMEKRHNYVRKTAELATQYYINPATSQPNVSGLILAGSADFKTELSQS 240

Query: 241 DMFDPRLQAKILNVVDVSYGGENGFNQAIELSSEILSNVKFIQEKRLIGKYFEEISQDTG 300
           DMFDPRL AKILNVVDVSYGGENGFNQAIELS+EIL+NVKFIQEKRLIGKYFEEISQDTG
Sbjct: 241 DMFDPRLAAKILNVVDVSYGGENGFNQAIELSAEILANVKFIQEKRLIGKYFEEISQDTG 300

Query: 301 KYVFGVDDTLKALEMGAVEILIVWENLDINRYVLKNAATGEIAIKHLNKEQEANQSNFRD 360
           KYVFGV+DTL ALE GA+E LIVWENLDINRYV+KN+ATGE  IKHLNKEQEAN  NF+ 
Sbjct: 301 KYVFGVEDTLNALESGAIETLIVWENLDINRYVMKNSATGETVIKHLNKEQEANTENFKV 360

Query: 361 PVSSAELEVQEKMALLEWFANEYKQFGCTLEFVTNKSQEGSQFCRGFGGIGGILRYQLDI 420
             S   L+V+EK++LLEW ANEY++FGC LEFVTNKSQEGSQFCRGFGGIGGILRYQLD+
Sbjct: 361 ADSDLALDVEEKLSLLEWLANEYRRFGCALEFVTNKSQEGSQFCRGFGGIGGILRYQLDM 420

Query: 421 RSFDELSDGEEYGD 435
            +FD   DGE   D
Sbjct: 421 TAFDS-EDGEALDD 433

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P356141.6e-22890.37Eukaryotic peptide chain release factor subunit 1-3 OS=Arabidopsis thaliana OX=3... [more]
D2K7594.3e-22689.22Eukaryotic peptide chain release factor subunit 1-2 OS=Brassica oleracea var. bo... [more]
D2K7607.3e-22689.45Eukaryotic peptide chain release factor subunit 1-3 OS=Brassica oleracea var. bo... [more]
Q9LPV81.1e-22489.10Eukaryotic peptide chain release factor subunit 1-2 OS=Arabidopsis thaliana OX=3... [more]
Q390972.2e-22288.25Eukaryotic peptide chain release factor subunit 1-1 OS=Arabidopsis thaliana OX=3... [more]
Match NameE-valueIdentityDescription
XP_022148872.12.1e-24699.08eukaryotic peptide chain release factor subunit 1-3-like [Momordica charantia][more]
KAG7014612.13.0e-24598.17Eukaryotic peptide chain release factor subunit 1-3, partial [Cucurbita argyrosp... [more]
KAG6576095.13.0e-24598.17Eukaryotic peptide chain release factor subunit 1-3, partial [Cucurbita argyrosp... [more]
XP_023548291.14.0e-24597.94eukaryotic peptide chain release factor subunit 1-3 [Cucurbita pepo subsp. pepo][more]
XP_022953222.18.9e-24597.94eukaryotic peptide chain release factor subunit 1-3 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
A0A6J1D6771.0e-24699.08eukaryotic peptide chain release factor subunit 1-3-like OS=Momordica charantia ... [more]
A0A6J1GMN24.3e-24597.94eukaryotic peptide chain release factor subunit 1-3 OS=Cucurbita moschata OX=366... [more]
A0A6J1H9461.2e-24498.17eukaryotic peptide chain release factor subunit 1-3-like OS=Cucurbita moschata O... [more]
A0A0A0KF541.6e-24498.17eRF1_1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G089230 PE=3... [more]
A0A6J1JV142.8e-24497.71eukaryotic peptide chain release factor subunit 1-3 OS=Cucurbita maxima OX=3661 ... [more]
Match NameE-valueIdentityDescription
AT3G26618.11.1e-22990.37eukaryotic release factor 1-3 [more]
AT1G12920.17.5e-22689.10eukaryotic release factor 1-2 [more]
AT5G47880.11.6e-22388.25eukaryotic release factor 1-1 [more]
AT5G47880.21.6e-22388.25eukaryotic release factor 1-1 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005140eRF1 domain 1/Pelota-likeSMARTSM01194eRF1_1_2coord: 4..140
e-value: 1.6E-47
score: 173.9
IPR005140eRF1 domain 1/Pelota-likePFAMPF03463eRF1_1coord: 17..138
e-value: 3.4E-20
score: 72.3
IPR02906450S ribosomal protein L30e-likeGENE3D3.30.1330.30coord: 279..436
e-value: 1.3E-65
score: 222.2
IPR02906450S ribosomal protein L30e-likeSUPERFAMILY55315L30e-likecoord: 278..420
IPR005142eRF1 domain 3PFAMPF03465eRF1_3coord: 281..418
e-value: 5.5E-36
score: 123.5
IPR042226eRF1, domain 2 superfamilyGENE3D3.30.420.60eRF1 domain 2coord: 136..277
e-value: 5.1E-61
score: 207.0
IPR005141eRF1 domain 2PFAMPF03464eRF1_2coord: 144..277
e-value: 2.2E-42
score: 144.5
IPR024049eRF1, domain 1GENE3D3.30.960.10eRF1 domain 1coord: 2..135
e-value: 4.9E-67
score: 225.9
IPR024049eRF1, domain 1SUPERFAMILY55481N-terminal domain of eukaryotic peptide chain release factor subunit 1, ERF1coord: 6..140
IPR004403Peptide chain release factor eRF1/aRF1TIGRFAMTIGR03676TIGR03676coord: 12..417
e-value: 1.1E-127
score: 424.2
IPR004403Peptide chain release factor eRF1/aRF1PANTHERPTHR10113PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1coord: 1..436
NoneNo IPR availablePANTHERPTHR10113:SF32EUKARYOTIC PEPTIDE CHAIN RELEASE FACTOR SUBUNIT 1-3-LIKEcoord: 1..436
NoneNo IPR availableSUPERFAMILY53137Translational machinery componentscoord: 142..276

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0004530.1Tan0004530.1mRNA
Tan0004530.2Tan0004530.2mRNA
Tan0004530.3Tan0004530.3mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002184 cytoplasmic translational termination
biological_process GO:0040008 regulation of growth
biological_process GO:0006415 translational termination
cellular_component GO:0005829 cytosol
cellular_component GO:0018444 translation release factor complex
molecular_function GO:1990825 sequence-specific mRNA binding
molecular_function GO:0016149 translation release factor activity, codon specific
molecular_function GO:0003747 translation release factor activity