Tan0004461 (gene) Snake gourd v1

Overview
NameTan0004461
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionF-box protein PP2-B15-like
LocationLG08: 6252713 .. 6253834 (-)
RNA-Seq ExpressionTan0004461
SyntenyTan0004461
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSexon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCGGAATTTTGAGCATCGAAATGTTACCGGAGGACTGTGTTACGACGATTCTGTCACTCACGACTCCCTCCGACGCCGGAAAATTATCCCTCGTCTCGTCGACGTTCCGGTCAGCGGCGGAATCCGACGTCGTTTGGGACAGATTCTTGCCCGAAAACTACGCCCAAATTGTGGCCGGATCTGATATCTCCGACGAGGTCCCATTTCGTTCGAAGAGAGAGCTTTTCTTCCGATTCTGCTCGGCGATCCTTATCGACGGCGGTAAAAAGGTAAATAAATAAAACCCATAAAATTTTACCTCTTCCATTTTCTCGGAATCCAAACAAACATAAACAAATTATAAATTATTTATATATATATATGTGTGTTTTTTTTTTTTTGCAGAGCTTTGAATTGGAAAAAAATGGGGAAAATCAGTTATATATTATCAGCAAGAGAATTGGATATTACATGGAGCTCTGATCCACTCTGTTGGAGCTGGAAATCACATCCTCAATCCAGGTATATATATATATACAATTTTTTTTAAAACAATTATTATAACCTTTATTCTTATTTTGAATTGATAAGCCTGTTATTTTTAATTACCCCTTTTTTTTTGTTTTTATGTGTAAAGATTTGCAGAAGTGGCAGAGCTAAGAACAGTGAGTTGGCTAGAAATAAATGGAAAAATAAGAACAAAAAGTCTATCATCAAACACATTATATGGAGCTTATCTTTTATTGAAGATTTCAGAAAGAGCTTATGGATTGGATTTGATAGCAGCTCAAGTTTCAGTTCAATTGGGAAATCAATTACAAATTTCTCAATCCTCTGTTTGGCTTCAACACAAAGACAAGAAAAACCAAGATTTAGAATGTTTGTTTTATGGGAATAGAAGACAAAGGCTTAGAAATTTAATGGGATCTGATAAATTATTACAAAATCATCAAAATGAATCCATCAAAAAGGATTTGAAAGAGAGACAAGATGGTTGGTTTGAGATTGAATTGGGTCAGTTCTTCATTGCTCAAAATCAAAATGATGAACAAGTTCTAATCACTTTAATGGAGACTAAAGGCTTTCAACTCAAATCTGGACTTATTCTTGAAGGCATTCAAATTAGACCTAAATATTAA

mRNA sequence

ATGGCCGGAATTTTGAGCATCGAAATGTTACCGGAGGACTGTGTTACGACGATTCTGTCACTCACGACTCCCTCCGACGCCGGAAAATTATCCCTCGTCTCGTCGACGTTCCGGTCAGCGGCGGAATCCGACGTCGTTTGGGACAGATTCTTGCCCGAAAACTACGCCCAAATTGTGGCCGGATCTGATATCTCCGACGAGGTCCCATTTCGTTCGAAGAGAGAGCTTTTCTTCCGATTCTGCTCGGCGATCCTTATCGACGGCGCTTTGAATTGGAAAAAAATGGGGAAAATCAGTTATATATTATCAGCAAGAGAATTGGATATTACATGGAGCTCTGATCCACTCTGTTGGAGCTGGAAATCACATCCTCAATCCAGATTTGCAGAAGTGGCAGAGCTAAGAACAGTGAGTTGGCTAGAAATAAATGGAAAAATAAGAACAAAAAGTCTATCATCAAACACATTATATGGAGCTTATCTTTTATTGAAGATTTCAGAAAGAGCTTATGGATTGGATTTGATAGCAGCTCAAGTTTCAGTTCAATTGGGAAATCAATTACAAATTTCTCAATCCTCTGTTTGGCTTCAACACAAAGACAAGAAAAACCAAGATTTAGAATGTTTGTTTTATGGGAATAGAAGACAAAGGCTTAGAAATTTAATGGGATCTGATAAATTATTACAAAATCATCAAAATGAATCCATCAAAAAGGATTTGAAAGAGAGACAAGATGGTTGGTTTGAGATTGAATTGGGTCAGTTCTTCATTGCTCAAAATCAAAATGATGAACAAGTTCTAATCACTTTAATGGAGACTAAAGGCTTTCAACTCAAATCTGGACTTATTCTTGAAGGCATTCAAATTAGACCTAAATATTAA

Coding sequence (CDS)

ATGGCCGGAATTTTGAGCATCGAAATGTTACCGGAGGACTGTGTTACGACGATTCTGTCACTCACGACTCCCTCCGACGCCGGAAAATTATCCCTCGTCTCGTCGACGTTCCGGTCAGCGGCGGAATCCGACGTCGTTTGGGACAGATTCTTGCCCGAAAACTACGCCCAAATTGTGGCCGGATCTGATATCTCCGACGAGGTCCCATTTCGTTCGAAGAGAGAGCTTTTCTTCCGATTCTGCTCGGCGATCCTTATCGACGGCGCTTTGAATTGGAAAAAAATGGGGAAAATCAGTTATATATTATCAGCAAGAGAATTGGATATTACATGGAGCTCTGATCCACTCTGTTGGAGCTGGAAATCACATCCTCAATCCAGATTTGCAGAAGTGGCAGAGCTAAGAACAGTGAGTTGGCTAGAAATAAATGGAAAAATAAGAACAAAAAGTCTATCATCAAACACATTATATGGAGCTTATCTTTTATTGAAGATTTCAGAAAGAGCTTATGGATTGGATTTGATAGCAGCTCAAGTTTCAGTTCAATTGGGAAATCAATTACAAATTTCTCAATCCTCTGTTTGGCTTCAACACAAAGACAAGAAAAACCAAGATTTAGAATGTTTGTTTTATGGGAATAGAAGACAAAGGCTTAGAAATTTAATGGGATCTGATAAATTATTACAAAATCATCAAAATGAATCCATCAAAAAGGATTTGAAAGAGAGACAAGATGGTTGGTTTGAGATTGAATTGGGTCAGTTCTTCATTGCTCAAAATCAAAATGATGAACAAGTTCTAATCACTTTAATGGAGACTAAAGGCTTTCAACTCAAATCTGGACTTATTCTTGAAGGCATTCAAATTAGACCTAAATATTAA

Protein sequence

MAGILSIEMLPEDCVTTILSLTTPSDAGKLSLVSSTFRSAAESDVVWDRFLPENYAQIVAGSDISDEVPFRSKRELFFRFCSAILIDGALNWKKMGKISYILSARELDITWSSDPLCWSWKSHPQSRFAEVAELRTVSWLEINGKIRTKSLSSNTLYGAYLLLKISERAYGLDLIAAQVSVQLGNQLQISQSSVWLQHKDKKNQDLECLFYGNRRQRLRNLMGSDKLLQNHQNESIKKDLKERQDGWFEIELGQFFIAQNQNDEQVLITLMETKGFQLKSGLILEGIQIRPKY
Homology
BLAST of Tan0004461 vs. ExPASy Swiss-Prot
Match: O80494 (F-box protein PP2-B15 OS=Arabidopsis thaliana OX=3702 GN=PP2B15 PE=2 SV=2)

HSP 1 Score: 240.4 bits (612), Expect = 2.7e-62
Identity = 131/290 (45.17%), Postives = 186/290 (64.14%), Query Frame = 0

Query: 9   MLPEDCVTTILSLTTPSDAGKLSLVSSTFRSAAESDVVWDRFLPENYAQIVAGSDISDEV 68
           MLPE CV TILS TTP+D    + VSS FR A +SD VW++FLP +Y  +++ S     +
Sbjct: 2   MLPEACVATILSFTTPADTISSAAVSSVFRVAGDSDFVWEKFLPTDYCHVISRSTDPHRI 61

Query: 69  PFRSKRELFFRFCSAILIDGALNWKKM----GKISYILSARELDITWSSDPLCWSWKSHP 128
            F SK+EL+   C +ILID      K+    GKISYILS+R+L ITWS     WSW    
Sbjct: 62  -FSSKKELYRCLCESILIDNGRKIFKIEKLSGKISYILSSRDLSITWSDQRHYWSWSPRS 121

Query: 129 QSRFAEVAELRTVSWLEINGKIRTKSLSSNTLYGAYLLLKISERAYGLDLIAAQVSVQLG 188
            SRF+E  +L    WLEI GKI+T +LS NT YGAYL++K++ RAYGLDL+ A+ S+++G
Sbjct: 122 DSRFSEGVQLIMTDWLEIIGKIQTGALSPNTNYGAYLIMKVTSRAYGLDLVPAETSIKVG 181

Query: 189 NQLQISQSSVWLQHKDKKNQDLECLFYGNRRQRLRNLMGSDKLLQNHQNESIKKDLKERQ 248
           N  +    S +L   D K Q +E +FYG R QR    M + +++++H+ E      + R 
Sbjct: 182 NG-EKKIKSTYLSCLDNKKQQMERVFYGQREQR----MATHEVVRSHRREP-----EVRD 241

Query: 249 DGWFEIELGQFFI--AQNQNDEQVLITLMETKGFQLKSGLILEGIQIRPK 293
           DGW EIELG+F     +  +D++V+++L E KG+QLK G+ ++GI++RPK
Sbjct: 242 DGWMEIELGEFETGSGEGDDDKEVVMSLTEVKGYQLKGGIAIDGIEVRPK 280

BLAST of Tan0004461 vs. ExPASy Swiss-Prot
Match: Q9C7K0 (F-box protein VBF OS=Arabidopsis thaliana OX=3702 GN=VBF PE=1 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 1.0e-58
Identity = 127/288 (44.10%), Postives = 187/288 (64.93%), Query Frame = 0

Query: 9   MLPEDCVTTILSLTTPSDAGKLSLVSSTFRSAAESDVVWDRFLPENYAQIVAGSDISDEV 68
           MLPE C+  IL+ T+P+DA   S VSS FR A +SD VW++FLP +Y  +++ S      
Sbjct: 3   MLPEACIANILAFTSPADAFSSSEVSSVFRLAGDSDFVWEKFLPSDYKSLISQS-TDHHW 62

Query: 69  PFRSKRELFFRFCSAILIDGALNWKKM----GKISYILSARELDITWSSDPLCWSWKSHP 128
              SK+E++   C ++LID A    K+    GKISY+LSAR++ IT S     WSW +  
Sbjct: 63  NISSKKEIYRCLCDSLLIDNARKLFKINKFSGKISYVLSARDISITHSDHASYWSWSNVS 122

Query: 129 QSRFAEVAELRTVSWLEINGKIRTKSLSSNTLYGAYLLLKISERAYGLDLIAAQVSVQLG 188
            SRF+E AEL     LEI GKI+T+ LS+NT YGAYL++K+++ AYGLDL+ A+ S++  
Sbjct: 123 DSRFSESAELIITDRLEIEGKIQTRVLSANTRYGAYLIVKVTKGAYGLDLVPAETSIKSK 182

Query: 189 NQLQISQSSVWLQHKDKKNQDLECLFYGNRRQRLRNLMGSDKLLQNHQNESIKKDLKERQ 248
           N  QIS+S+ +L   D+K Q ++ LFYGNR +R+         ++    +  +++ K R 
Sbjct: 183 NG-QISKSATYLCCLDEKKQQMKRLFYGNREERMA------MTVEAVGGDGKRREPKCRD 242

Query: 249 DGWFEIELGQFFIAQNQNDEQVLITLMETKGFQLKSGLILEGIQIRPK 293
           DGW EIELG+F   + ++DE V +TL E KG+QLK G++++GI++RPK
Sbjct: 243 DGWMEIELGEFETREGEDDE-VNMTLTEVKGYQLKGGILIDGIEVRPK 281

BLAST of Tan0004461 vs. ExPASy Swiss-Prot
Match: Q9C7J9 (F-box protein PP2-B13 OS=Arabidopsis thaliana OX=3702 GN=PP2B13 PE=2 SV=1)

HSP 1 Score: 224.2 bits (570), Expect = 2.0e-57
Identity = 126/287 (43.90%), Postives = 185/287 (64.46%), Query Frame = 0

Query: 9   MLPEDCVTTILSLTTPSDAGKLSLVSSTFRSAAESDVVWDRFLPENYAQIVAGSDISDEV 68
           MLPE CV  IL+ T+P+DA   S VSS FR A +SD VW++FLP +Y  +++ S     +
Sbjct: 3   MLPEACVANILAFTSPADAFSSSEVSSVFRLAGDSDFVWEKFLPSHYKSLISQSTDHHRI 62

Query: 69  PFRSKRELFFRFCSAILIDGALNWKKM----GKISYILSARELDITWSSDPLCWSWKSHP 128
            F SK+E++   C ++LID A    K+    GKISYILSAR++ IT+S      SW +  
Sbjct: 63  -FSSKKEIYRCLCDSLLIDNARKLFKINKFSGKISYILSARDISITYSDHASYCSWSNVS 122

Query: 129 QSRFAEVAELRTVSWLEINGKIRTKSLSSNTLYGAYLLLKISERAYGLDLIAAQVSVQLG 188
            SRF+E AEL T   LEI GKI+T  LS NT YGAYL++K++  AYGLDL+ A+ SV+  
Sbjct: 123 DSRFSESAELITTDRLEIKGKIQTTVLSPNTKYGAYLIMKVTNGAYGLDLVPAETSVKSK 182

Query: 189 NQLQISQSSVWLQHKDKKNQDLECLFYGNRRQRLRNLMGSDKLLQNHQNESIKKDLKERQ 248
           N  Q ++++ +L   D+K Q ++ LFYGNR +R+         ++    +  +++ K R 
Sbjct: 183 NG-QNNKNTTYLCCLDEKKQQMKRLFYGNREERMA------MTVEAVGGDGKRREPKARD 242

Query: 249 DGWFEIELGQFFIAQNQNDEQVLITLMETKGFQLKSGLILEGIQIRP 292
           DGW EIELG+F   + ++DE V ++L E KG+QLK G++++GI++RP
Sbjct: 243 DGWLEIELGEFVTREGEDDE-VNMSLTEVKGYQLKGGIVIDGIEVRP 280

BLAST of Tan0004461 vs. ExPASy Swiss-Prot
Match: Q3E6P4 (F-box protein At2g02240 OS=Arabidopsis thaliana OX=3702 GN=At2g02240 PE=2 SV=1)

HSP 1 Score: 172.2 bits (435), Expect = 8.9e-42
Identity = 104/294 (35.37%), Postives = 156/294 (53.06%), Query Frame = 0

Query: 3   GILSIEMLPEDCVTTILSLTTPSDAGKLSLVSSTFRSAAESDVVWDRFLPENYAQIVAGS 62
           G    ++LPEDC++ I+S T+P DA   + VS TF SA  SD VWD+FLP  Y  +V+ S
Sbjct: 57  GTSPFDVLPEDCISNIISFTSPRDACVAASVSKTFESAVSSDCVWDKFLPPEYESLVSRS 116

Query: 63  DISDEVPFRSKRELFFRFC--SAILIDGALNW---KKMGKISYILSARELDITWSSDPLC 122
            +     F SK+EL+F  C    ++ DG  ++   K  GK   +LS++EL ITW S P  
Sbjct: 117 RV-----FASKKELYFALCHNPVLIEDGKKSFWLEKASGKRCIMLSSKELWITWGSSPEY 176

Query: 123 WSWKSHPQSRFAEVAELRTVSWLEINGKIRTKSLSSNTLYGAYLLLKISERAYGLDLIAA 182
           W W S P+SRF ++AEL  V W EI GK   + LS  T Y AY++ K  +R  GL  +  
Sbjct: 177 WQWISIPESRFNKIAELLDVCWFEIRGKTSARVLSPGTRYSAYIVFKTKDRCPGLGHLPV 236

Query: 183 QVSVQLGNQLQISQSSVWLQHKDKKNQDLECLFYGNRRQRLRNLMGSDKLLQNHQNESIK 242
           +V + L  Q    +   ++  +D++            R+  R++   D            
Sbjct: 237 EVGLGLVGQESSKRFIYFIGPRDRRG-----------RRETRDVTKPD------------ 296

Query: 243 KDLKERQDGWFEIELGQFFIAQNQNDEQVLITLMETKGFQLKSGLILEGIQIRP 292
               +R+DGW E ELG+FF    +  +++  +++E K    KSGLI++GI+ RP
Sbjct: 297 ----QREDGWMEAELGEFF--NEERCDEIEFSVIEIKSPSWKSGLIIQGIEFRP 316

BLAST of Tan0004461 vs. ExPASy Swiss-Prot
Match: Q6NPT8 (F-box protein PP2-B1 OS=Arabidopsis thaliana OX=3702 GN=PP2B1 PE=1 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.7e-40
Identity = 105/310 (33.87%), Postives = 170/310 (54.84%), Query Frame = 0

Query: 8   EMLPEDCVTTILSLTTPSDAGKLSLVSSTFRSAAESDVVWDRFLPENYAQIVAGSDISDE 67
           + LPEDC++ ++S T+P DA  ++ VS + +SAA+SD+VW+ FLP  Y+ +V  S     
Sbjct: 33  DALPEDCISKVISHTSPRDACVVASVSKSVKSAAQSDLVWEMFLPSEYSSLVLQS----- 92

Query: 68  VPFRSKRELFFRFC-SAILIDGALN--W--KKMGKISYILSARELDITWSSDPLCWSWKS 127
               SK+E+F     +++L++      W  K  GK  Y+LSA EL I W   P  W W +
Sbjct: 93  ANHLSKKEIFLSLADNSVLVENGKKSFWVEKASGKKCYMLSAMELTIIWGDSPAYWKWIT 152

Query: 128 HPQSRFAEVAELRTVSWLEINGKIRTKSLSSNTLYGAYLLLKISE-RAYGLDLIAAQVSV 187
            P+S+F +VAELR V W E+ GKI    LS  T Y  Y++ K +  R+YG DL+  +  V
Sbjct: 153 VPESKFEKVAELRNVCWFEVRGKISCGMLSKGTHYSVYVVFKTANGRSYGFDLVPVEAGV 212

Query: 188 QLGNQLQISQSSVWLQHKDKKNQDLECLFYG------NRRQRLRNLMGSDKLLQNHQNES 247
               ++  ++ SV+ +  +  ++     + G      +R  R+R        +Q  + E 
Sbjct: 213 GFVGKV-ATKKSVYFESGNADSRSATSHYSGISYAMVSRAFRMRRPW---MQVQREEEEE 272

Query: 248 IKKDL---------KERQDGWFEIELGQFFI----AQNQNDEQVLITLMETKGFQLKSGL 293
           ++ +          KER DGW E+ELG+F+I      +   +++ I++MET+    KSGL
Sbjct: 273 VEGERERGMNVVGPKERVDGWSEVELGKFYINNGGCGDDGSDEIEISIMETQNGNWKSGL 332

BLAST of Tan0004461 vs. NCBI nr
Match: XP_022156423.1 (F-box protein VBF-like [Momordica charantia])

HSP 1 Score: 407.1 bits (1045), Expect = 1.2e-109
Identity = 208/299 (69.57%), Postives = 252/299 (84.28%), Query Frame = 0

Query: 1   MAGILSIEMLPEDCVTTILSLTTPSDAGKLSLVSSTFRSAAESDVVWDRFLPENYAQIVA 60
           MAGILSI MLPEDCV+TILSLTTPSD G+LS+VSS FRSAAESDVVW RFLP NYA I+A
Sbjct: 1   MAGILSIGMLPEDCVSTILSLTTPSDVGRLSVVSSAFRSAAESDVVWGRFLPNNYAHILA 60

Query: 61  GSDISDEVPFRSKRELFFRFCSAILIDG---ALNWKKM-GKISYILSARELDITWSSDPL 120
            +DIS E PF SKRE+FFR CS +L+DG   +L  +K+  KI Y LSAREL I+WSSDPL
Sbjct: 61  AADISGESPFCSKREIFFRLCSPLLLDGGKKSLELEKISAKICYTLSARELSISWSSDPL 120

Query: 121 CWSWKSHPQSRFAEVAELRTVSWLEINGKIRTKSLSSNTLYGAYLLLKISERAYGLDLIA 180
           CWSWK+H  SRFAEVAELRTVSWLEI+GKIRTK++SSNTLYGAYLL+KIS+RAYGLDL+ 
Sbjct: 121 CWSWKNHSHSRFAEVAELRTVSWLEISGKIRTKTVSSNTLYGAYLLVKISDRAYGLDLVP 180

Query: 181 AQVSVQLGN--QLQISQSSVWLQHKDKKNQDLECLFYGNRRQRLRNLMGSDKLLQNHQNE 240
           AQV VQ+G+  Q Q+S+ SVWLQ KDKK +D+ECLFYGNRR+R+R L+G+ + ++  +N 
Sbjct: 181 AQVCVQVGDGAQAQVSEGSVWLQRKDKKKEDIECLFYGNRRERVRKLVGNGEKME--ENG 240

Query: 241 SIKKDLKERQDGWFEIELGQFFIAQNQNDEQVLITLMETKGFQLKSGLILEGIQIRPKY 294
           SI+  LKER+DGW EIELG+FF  +N  DE+V ++LME KGFQLK+GL+L+GIQIRPK+
Sbjct: 241 SIRV-LKEREDGWLEIELGEFFSGENGTDEEVKMSLMEIKGFQLKAGLVLQGIQIRPKH 296

BLAST of Tan0004461 vs. NCBI nr
Match: XP_023537654.1 (F-box protein PP2-B15-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 394.4 bits (1012), Expect = 8.4e-106
Identity = 198/296 (66.89%), Postives = 242/296 (81.76%), Query Frame = 0

Query: 1   MAGILSIEMLPEDCVTTILSLTTPSDAGKLSLVSSTFRSAAESDVVWDRFLPENYAQIVA 60
           MAGI ++ MLP+DC++ ILSLTTP+D GK+SL+S  FR AAESDVVW RFLPENYA+I+ 
Sbjct: 1   MAGIFTVGMLPDDCLSAILSLTTPADVGKVSLISPAFRLAAESDVVWGRFLPENYARIIG 60

Query: 61  GSDISDEVPFRSKRELFFRFCSAILIDGALNW----KKMGKISYILSARELDITWSSDPL 120
            SDISDEVPF SKRE+FFR CS ILIDG        K  GKI YILSAREL ITWSSDPL
Sbjct: 61  ASDISDEVPFGSKREIFFRLCSPILIDGGKTSFELDKFSGKICYILSARELGITWSSDPL 120

Query: 121 CWSWKSHPQSRFAEVAELRTVSWLEINGKIRTKSLSSNTLYGAYLLLKISERAYGLDLIA 180
           CWSWK HP+SRF+EVAELRTVSWLEI+G+IR + LS NT YGAYLL+KI+ERAYGLDL+A
Sbjct: 121 CWSWKPHPESRFSEVAELRTVSWLEIHGRIRRRRLSPNTEYGAYLLVKITERAYGLDLMA 180

Query: 181 AQVSVQLGNQLQISQSSVWLQHKDKKNQDLECLFYGNRRQRLRNLMGSDKLLQNHQNESI 240
           A+VSV +GN  +IS+ SVWLQ K +K +DLE LFYGNRR+R+RNLMG++   +  ++  I
Sbjct: 181 AEVSVAVGNGGRISEGSVWLQRKQRKEEDLESLFYGNRRERVRNLMGTE---EEEESNGI 240

Query: 241 KKDLKERQDGWFEIELGQFFIAQNQNDEQVLITLMETKGFQLKSGLILEGIQIRPK 293
            + LKER DGWFEIELG+F   ++++DE V ++LME +GFQLK+G+I+EGIQIRPK
Sbjct: 241 -RGLKERDDGWFEIELGEFMSGEDEDDEDVHMSLMEIRGFQLKAGIIVEGIQIRPK 292

BLAST of Tan0004461 vs. NCBI nr
Match: KAG7021074.1 (F-box protein PP2-B15, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 393.7 bits (1010), Expect = 1.4e-105
Identity = 201/298 (67.45%), Postives = 242/298 (81.21%), Query Frame = 0

Query: 1   MAGILSIEMLPEDCVTTILSLTTPSDAGKLSLVSSTFRSAAESDVVWDRFLPENYAQIVA 60
           MAGI ++ MLP+DC++ ILSLTTP+D GKLSL+SS FR AAESDVVW RFLPENYA+I+A
Sbjct: 1   MAGIFTVGMLPDDCLSAILSLTTPADVGKLSLISSAFRPAAESDVVWGRFLPENYARIIA 60

Query: 61  GSDISDEVPFRSKRELFFRFCSAILIDGALNW----KKMGKISYILSARELDITWSSDPL 120
            SDISDEVPF SKRE+FFR CS ILIDG        K  GKI YILSAREL ITWSSDPL
Sbjct: 61  ASDISDEVPFGSKREIFFRLCSPILIDGGKTSFELDKFSGKICYILSARELGITWSSDPL 120

Query: 121 CWSWKSHPQSRFAEVAELRTVSWLEINGKIRTKSLSSNTLYGAYLLLKISERAYGLDLIA 180
           CWSWK HP+SRF+EVAELRTVSWLEI+G+IR + LS NT YGAYLL+KI+ERAYGLDL+A
Sbjct: 121 CWSWKPHPESRFSEVAELRTVSWLEIHGRIRRQRLSPNTEYGAYLLVKITERAYGLDLMA 180

Query: 181 AQVSVQLGNQLQISQSSVWLQHKDKKNQDLECLFYGNRRQRLRNLMGSDKLLQNHQNESI 240
           A+VSV +GN  +IS+  VWLQ K +K +DLE LFYGNRR+R+RNLMG++   +  +N  I
Sbjct: 181 AEVSVAVGNGGRISEGRVWLQRKQRKEEDLESLFYGNRRERVRNLMGTE---EEEENNGI 240

Query: 241 KKDLKERQDGWFEIELGQFFIAQ--NQNDEQVLITLMETKGFQLKSGLILEGIQIRPK 293
            + LKER DGWFEIELG+F   +  + N+E V ++LME +GFQLK+G+I+EGIQIRPK
Sbjct: 241 -RGLKERDDGWFEIELGEFMSGEDDDDNEEDVHMSLMEIRGFQLKAGIIVEGIQIRPK 294

BLAST of Tan0004461 vs. NCBI nr
Match: XP_022937763.1 (F-box protein PP2-B15-like [Cucurbita moschata] >KAG6586239.1 F-box protein PP2-B15, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 392.9 bits (1008), Expect = 2.4e-105
Identity = 201/299 (67.22%), Postives = 242/299 (80.94%), Query Frame = 0

Query: 1   MAGILSIEMLPEDCVTTILSLTTPSDAGKLSLVSSTFRSAAESDVVWDRFLPENYAQIVA 60
           MAGI ++ MLP+DC++ ILSLTTP+D GKLSL+SS FR AAESDVVW RFLPENYA+I+A
Sbjct: 1   MAGIFTVGMLPDDCLSAILSLTTPADVGKLSLISSAFRPAAESDVVWGRFLPENYARIIA 60

Query: 61  GSDISDEVPFRSKRELFFRFCSAILIDGALNW----KKMGKISYILSARELDITWSSDPL 120
            SDISDEVPF SKRE+FFR CS ILIDG        K  GKI YILSAREL ITWSSDPL
Sbjct: 61  ASDISDEVPFGSKREIFFRLCSPILIDGGKTSFELDKFSGKICYILSARELGITWSSDPL 120

Query: 121 CWSWKSHPQSRFAEVAELRTVSWLEINGKIRTKSLSSNTLYGAYLLLKISERAYGLDLIA 180
           CWSWK HP+SRF+EVAELRTVSWLEI+G+IR + LS NT YGAYLL+KI+ERAYGLDL+A
Sbjct: 121 CWSWKPHPESRFSEVAELRTVSWLEIHGRIRRQRLSPNTEYGAYLLVKITERAYGLDLMA 180

Query: 181 AQVSVQLGNQLQISQSSVWLQHKDKKNQDLECLFYGNRRQRLRNLMGSDKLLQNHQNESI 240
           A+VSV +GN  +IS+  VWLQ K +K +DLE LFYGNRR+R+RNLMG++   +  +N  I
Sbjct: 181 AEVSVAVGNGGRISEGRVWLQRKQRKEEDLESLFYGNRRERVRNLMGTE---EEEENNGI 240

Query: 241 KKDLKERQDGWFEIELGQFFIAQNQND---EQVLITLMETKGFQLKSGLILEGIQIRPK 293
            + LKER DGWFEIELG+F   ++ +D   E V ++LME +GFQLK+G+I+EGIQIRPK
Sbjct: 241 -RGLKERDDGWFEIELGEFMSGEDDDDDDEEDVHMSLMEIRGFQLKAGIIVEGIQIRPK 295

BLAST of Tan0004461 vs. NCBI nr
Match: XP_022156417.1 (F-box protein VBF-like [Momordica charantia])

HSP 1 Score: 392.5 bits (1007), Expect = 3.2e-105
Identity = 205/299 (68.56%), Postives = 245/299 (81.94%), Query Frame = 0

Query: 1   MAGILSIEMLPEDCVTTILSLTTPSDAGKLSLVSSTFRSAAESDVVWDRFLPENYAQIVA 60
           MAGI SI MLPEDCV+ ILSLTTPSDAG+LS+VS+ FRSAAESDVVW RFLP+NYA I+A
Sbjct: 1   MAGIFSIGMLPEDCVSEILSLTTPSDAGRLSVVSAAFRSAAESDVVWGRFLPDNYAHILA 60

Query: 61  GSDISDEVPFRSKRELFFRFCSAILIDGALN----WKKMGKISYILSARELDITWSSDPL 120
            +DIS E PF SKRE+FF FCS +L+DG        K  GKISYILSAREL IT SSDP 
Sbjct: 61  AADISGESPFCSKREIFFLFCSPLLLDGGKKSFQLEKFSGKISYILSARELSITGSSDPX 120

Query: 121 CWSWKSHPQSRFAEVAELRTVSWLEINGKIRTKSLSSNTLYGAYLLLKISERAYGLDLIA 180
           CWSW +H QSRFAEVAELRTVS LEINGKI TK+LSSNTLYGAYLL+KIS+ AYGLDL+ 
Sbjct: 121 CWSWNNHSQSRFAEVAELRTVSRLEINGKIGTKTLSSNTLYGAYLLVKISDWAYGLDLVP 180

Query: 181 AQVSVQLGN--QLQISQSSVWLQHKDKKNQDLECLFYGNRRQRLRNLMGSDKLLQNHQNE 240
           AQV VQ+G+  Q Q+S+ SVWLQ KDKK +D+ECLFYGNRR+R+R L+ + + ++  +N 
Sbjct: 181 AQVCVQVGDGAQAQVSEGSVWLQRKDKKKEDMECLFYGNRRERVRKLVENGEKME--ENG 240

Query: 241 SIKKDLKERQDGWFEIELGQFFIAQNQNDEQVLITLMETKGFQLKSGLILEGIQIRPKY 294
           SI+  LKER+DGW EIELG+FF  +N  DE+V ++LME KGFQLK+GL+L+GIQIRPK+
Sbjct: 241 SIRV-LKEREDGWLEIELGEFFSGENGTDEEVKMSLMEIKGFQLKAGLVLQGIQIRPKH 296

BLAST of Tan0004461 vs. ExPASy TrEMBL
Match: A0A6J1DQK9 (F-box protein VBF-like OS=Momordica charantia OX=3673 GN=LOC111023316 PE=4 SV=1)

HSP 1 Score: 407.1 bits (1045), Expect = 6.0e-110
Identity = 208/299 (69.57%), Postives = 252/299 (84.28%), Query Frame = 0

Query: 1   MAGILSIEMLPEDCVTTILSLTTPSDAGKLSLVSSTFRSAAESDVVWDRFLPENYAQIVA 60
           MAGILSI MLPEDCV+TILSLTTPSD G+LS+VSS FRSAAESDVVW RFLP NYA I+A
Sbjct: 1   MAGILSIGMLPEDCVSTILSLTTPSDVGRLSVVSSAFRSAAESDVVWGRFLPNNYAHILA 60

Query: 61  GSDISDEVPFRSKRELFFRFCSAILIDG---ALNWKKM-GKISYILSARELDITWSSDPL 120
            +DIS E PF SKRE+FFR CS +L+DG   +L  +K+  KI Y LSAREL I+WSSDPL
Sbjct: 61  AADISGESPFCSKREIFFRLCSPLLLDGGKKSLELEKISAKICYTLSARELSISWSSDPL 120

Query: 121 CWSWKSHPQSRFAEVAELRTVSWLEINGKIRTKSLSSNTLYGAYLLLKISERAYGLDLIA 180
           CWSWK+H  SRFAEVAELRTVSWLEI+GKIRTK++SSNTLYGAYLL+KIS+RAYGLDL+ 
Sbjct: 121 CWSWKNHSHSRFAEVAELRTVSWLEISGKIRTKTVSSNTLYGAYLLVKISDRAYGLDLVP 180

Query: 181 AQVSVQLGN--QLQISQSSVWLQHKDKKNQDLECLFYGNRRQRLRNLMGSDKLLQNHQNE 240
           AQV VQ+G+  Q Q+S+ SVWLQ KDKK +D+ECLFYGNRR+R+R L+G+ + ++  +N 
Sbjct: 181 AQVCVQVGDGAQAQVSEGSVWLQRKDKKKEDIECLFYGNRRERVRKLVGNGEKME--ENG 240

Query: 241 SIKKDLKERQDGWFEIELGQFFIAQNQNDEQVLITLMETKGFQLKSGLILEGIQIRPKY 294
           SI+  LKER+DGW EIELG+FF  +N  DE+V ++LME KGFQLK+GL+L+GIQIRPK+
Sbjct: 241 SIRV-LKEREDGWLEIELGEFFSGENGTDEEVKMSLMEIKGFQLKAGLVLQGIQIRPKH 296

BLAST of Tan0004461 vs. ExPASy TrEMBL
Match: A0A6J1FGV3 (F-box protein PP2-B15-like OS=Cucurbita moschata OX=3662 GN=LOC111444065 PE=4 SV=1)

HSP 1 Score: 392.9 bits (1008), Expect = 1.2e-105
Identity = 201/299 (67.22%), Postives = 242/299 (80.94%), Query Frame = 0

Query: 1   MAGILSIEMLPEDCVTTILSLTTPSDAGKLSLVSSTFRSAAESDVVWDRFLPENYAQIVA 60
           MAGI ++ MLP+DC++ ILSLTTP+D GKLSL+SS FR AAESDVVW RFLPENYA+I+A
Sbjct: 1   MAGIFTVGMLPDDCLSAILSLTTPADVGKLSLISSAFRPAAESDVVWGRFLPENYARIIA 60

Query: 61  GSDISDEVPFRSKRELFFRFCSAILIDGALNW----KKMGKISYILSARELDITWSSDPL 120
            SDISDEVPF SKRE+FFR CS ILIDG        K  GKI YILSAREL ITWSSDPL
Sbjct: 61  ASDISDEVPFGSKREIFFRLCSPILIDGGKTSFELDKFSGKICYILSARELGITWSSDPL 120

Query: 121 CWSWKSHPQSRFAEVAELRTVSWLEINGKIRTKSLSSNTLYGAYLLLKISERAYGLDLIA 180
           CWSWK HP+SRF+EVAELRTVSWLEI+G+IR + LS NT YGAYLL+KI+ERAYGLDL+A
Sbjct: 121 CWSWKPHPESRFSEVAELRTVSWLEIHGRIRRQRLSPNTEYGAYLLVKITERAYGLDLMA 180

Query: 181 AQVSVQLGNQLQISQSSVWLQHKDKKNQDLECLFYGNRRQRLRNLMGSDKLLQNHQNESI 240
           A+VSV +GN  +IS+  VWLQ K +K +DLE LFYGNRR+R+RNLMG++   +  +N  I
Sbjct: 181 AEVSVAVGNGGRISEGRVWLQRKQRKEEDLESLFYGNRRERVRNLMGTE---EEEENNGI 240

Query: 241 KKDLKERQDGWFEIELGQFFIAQNQND---EQVLITLMETKGFQLKSGLILEGIQIRPK 293
            + LKER DGWFEIELG+F   ++ +D   E V ++LME +GFQLK+G+I+EGIQIRPK
Sbjct: 241 -RGLKERDDGWFEIELGEFMSGEDDDDDDEEDVHMSLMEIRGFQLKAGIIVEGIQIRPK 295

BLAST of Tan0004461 vs. ExPASy TrEMBL
Match: A0A6J1DS06 (F-box protein VBF-like OS=Momordica charantia OX=3673 GN=LOC111023312 PE=4 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 1.5e-105
Identity = 205/299 (68.56%), Postives = 245/299 (81.94%), Query Frame = 0

Query: 1   MAGILSIEMLPEDCVTTILSLTTPSDAGKLSLVSSTFRSAAESDVVWDRFLPENYAQIVA 60
           MAGI SI MLPEDCV+ ILSLTTPSDAG+LS+VS+ FRSAAESDVVW RFLP+NYA I+A
Sbjct: 1   MAGIFSIGMLPEDCVSEILSLTTPSDAGRLSVVSAAFRSAAESDVVWGRFLPDNYAHILA 60

Query: 61  GSDISDEVPFRSKRELFFRFCSAILIDGALN----WKKMGKISYILSARELDITWSSDPL 120
            +DIS E PF SKRE+FF FCS +L+DG        K  GKISYILSAREL IT SSDP 
Sbjct: 61  AADISGESPFCSKREIFFLFCSPLLLDGGKKSFQLEKFSGKISYILSARELSITGSSDPX 120

Query: 121 CWSWKSHPQSRFAEVAELRTVSWLEINGKIRTKSLSSNTLYGAYLLLKISERAYGLDLIA 180
           CWSW +H QSRFAEVAELRTVS LEINGKI TK+LSSNTLYGAYLL+KIS+ AYGLDL+ 
Sbjct: 121 CWSWNNHSQSRFAEVAELRTVSRLEINGKIGTKTLSSNTLYGAYLLVKISDWAYGLDLVP 180

Query: 181 AQVSVQLGN--QLQISQSSVWLQHKDKKNQDLECLFYGNRRQRLRNLMGSDKLLQNHQNE 240
           AQV VQ+G+  Q Q+S+ SVWLQ KDKK +D+ECLFYGNRR+R+R L+ + + ++  +N 
Sbjct: 181 AQVCVQVGDGAQAQVSEGSVWLQRKDKKKEDMECLFYGNRRERVRKLVENGEKME--ENG 240

Query: 241 SIKKDLKERQDGWFEIELGQFFIAQNQNDEQVLITLMETKGFQLKSGLILEGIQIRPKY 294
           SI+  LKER+DGW EIELG+FF  +N  DE+V ++LME KGFQLK+GL+L+GIQIRPK+
Sbjct: 241 SIRV-LKEREDGWLEIELGEFFSGENGTDEEVKMSLMEIKGFQLKAGLVLQGIQIRPKH 296

BLAST of Tan0004461 vs. ExPASy TrEMBL
Match: A0A6J1HPX5 (F-box protein PP2-B15-like OS=Cucurbita maxima OX=3661 GN=LOC111465617 PE=4 SV=1)

HSP 1 Score: 384.8 bits (987), Expect = 3.2e-103
Identity = 198/296 (66.89%), Postives = 241/296 (81.42%), Query Frame = 0

Query: 1   MAGILSIEMLPEDCVTTILSLTTPSDAGKLSLVSSTFRSAAESDVVWDRFLPENYAQIVA 60
           MAGI ++ MLP+DC++ ILSLTTP+D  KLSL+SS FRS AESDVVW RFLPENYAQI+A
Sbjct: 1   MAGIFTVGMLPDDCLSAILSLTTPADVVKLSLISSEFRSVAESDVVWGRFLPENYAQIIA 60

Query: 61  GSDISDEVPFRSKRELFFRFCSAILID-GALNW---KKMGKISYILSARELDITWSSDPL 120
            SDISDEVPFRSKRE+FFR CS ILID G  ++   K  GKI Y+LSAREL ITWSSDPL
Sbjct: 61  ASDISDEVPFRSKREIFFRLCSPILIDSGKTSFELDKFSGKICYLLSARELGITWSSDPL 120

Query: 121 CWSWKSHPQSRFAEVAELRTVSWLEINGKIRTKSLSSNTLYGAYLLLKISERAYGLDLIA 180
           CWSWK HPQSRF+EVAELRTVSWLEI+G+I  + LS NT YGAYL++KI+ERAYGLDL+A
Sbjct: 121 CWSWKRHPQSRFSEVAELRTVSWLEIHGRIGRQKLSPNTEYGAYLIVKITERAYGLDLMA 180

Query: 181 AQVSVQLGNQLQISQSSVWLQHKDKKNQDLECLFYGNRRQRLRNLMGSDKLLQNHQNESI 240
           A+VSV +GN  +I++  VWLQ K +K +DLE LFYGNRR+R+RNLMG++   +  ++  I
Sbjct: 181 AEVSVAVGNGGRITEGRVWLQRKQRKEEDLESLFYGNRRERVRNLMGTE---EEEESNGI 240

Query: 241 KKDLKERQDGWFEIELGQFFIAQNQNDEQVLITLMETKGFQLKSGLILEGIQIRPK 293
            + LKER DGWFEIELG F     ++DE V ++LME +GFQLK+G+I+EGIQIRPK
Sbjct: 241 -RSLKERDDGWFEIELGVFM--SGEDDEDVHMSLMEIRGFQLKAGIIVEGIQIRPK 290

BLAST of Tan0004461 vs. ExPASy TrEMBL
Match: A0A6J1KF36 (F-box protein PP2-B15-like OS=Cucurbita maxima OX=3661 GN=LOC111493739 PE=4 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 8.2e-99
Identity = 194/297 (65.32%), Postives = 234/297 (78.79%), Query Frame = 0

Query: 1   MAGILSIEMLPEDCVTTILSLTTPSDAGKLSLVSSTFRSAAESDVVWDRFLPENYAQIVA 60
           MAGI SI +LPEDC++ ILSLTTP+DAGKLSLVSSTFRSAAESDVVW RFLP NYA+I+A
Sbjct: 1   MAGISSIGVLPEDCISAILSLTTPTDAGKLSLVSSTFRSAAESDVVWSRFLPRNYAEIMA 60

Query: 61  GSDISDEVPFRSKRELFFRFCSAILIDGALNWKKM----GKISYILSARELDITWSSDPL 120
            SDI+ E+P  SKRE FFR CS ILIDG     ++    GKI Y LSAREL ITWSSDPL
Sbjct: 61  VSDIAGELPLCSKREAFFRLCSPILIDGGKKSFELERFSGKICYTLSARELAITWSSDPL 120

Query: 121 CWSWKSHPQSRFAEVAELRTVSWLEINGKIRTKSLSSNTLYGAYLLLKISERAYGLDLIA 180
           CWSWKS PQSRFAEVAELR  SW+EI+G+ RT  LS NTLYGAYL++KISE+ YGLDL+ 
Sbjct: 121 CWSWKSDPQSRFAEVAELRASSWVEIHGRTRTGRLSPNTLYGAYLVVKISEKGYGLDLMP 180

Query: 181 AQVSVQLGNQLQISQSSVWLQHKDKKNQDLECLFYGNRRQRLRNLMGSDKLLQNHQNESI 240
           AQV VQLG+ LQ SQ SVWL+ K++ N  +E LFYGNRR+R   L+ SD L ++  +  I
Sbjct: 181 AQVCVQLGDALQSSQGSVWLRRKEQSNLKMESLFYGNRRERAIKLLASD-LGEDFDSNGI 240

Query: 241 KKDLKERQDGWFEIELGQFFIAQNQNDEQVLITLMETKGFQLKSGLILEGIQIRPKY 294
            +DL+ER DGW EIELG+FF    ++DE + ++ +ETKGFQLKSGL+++ IQIRPK+
Sbjct: 241 -RDLRERDDGWSEIELGEFF--TGEDDEHLTMSFLETKGFQLKSGLVVQAIQIRPKH 293

BLAST of Tan0004461 vs. TAIR 10
Match: AT1G09155.1 (phloem protein 2-B15 )

HSP 1 Score: 240.4 bits (612), Expect = 1.9e-63
Identity = 131/290 (45.17%), Postives = 186/290 (64.14%), Query Frame = 0

Query: 9   MLPEDCVTTILSLTTPSDAGKLSLVSSTFRSAAESDVVWDRFLPENYAQIVAGSDISDEV 68
           MLPE CV TILS TTP+D    + VSS FR A +SD VW++FLP +Y  +++ S     +
Sbjct: 2   MLPEACVATILSFTTPADTISSAAVSSVFRVAGDSDFVWEKFLPTDYCHVISRSTDPHRI 61

Query: 69  PFRSKRELFFRFCSAILIDGALNWKKM----GKISYILSARELDITWSSDPLCWSWKSHP 128
            F SK+EL+   C +ILID      K+    GKISYILS+R+L ITWS     WSW    
Sbjct: 62  -FSSKKELYRCLCESILIDNGRKIFKIEKLSGKISYILSSRDLSITWSDQRHYWSWSPRS 121

Query: 129 QSRFAEVAELRTVSWLEINGKIRTKSLSSNTLYGAYLLLKISERAYGLDLIAAQVSVQLG 188
            SRF+E  +L    WLEI GKI+T +LS NT YGAYL++K++ RAYGLDL+ A+ S+++G
Sbjct: 122 DSRFSEGVQLIMTDWLEIIGKIQTGALSPNTNYGAYLIMKVTSRAYGLDLVPAETSIKVG 181

Query: 189 NQLQISQSSVWLQHKDKKNQDLECLFYGNRRQRLRNLMGSDKLLQNHQNESIKKDLKERQ 248
           N  +    S +L   D K Q +E +FYG R QR    M + +++++H+ E      + R 
Sbjct: 182 NG-EKKIKSTYLSCLDNKKQQMERVFYGQREQR----MATHEVVRSHRREP-----EVRD 241

Query: 249 DGWFEIELGQFFI--AQNQNDEQVLITLMETKGFQLKSGLILEGIQIRPK 293
           DGW EIELG+F     +  +D++V+++L E KG+QLK G+ ++GI++RPK
Sbjct: 242 DGWMEIELGEFETGSGEGDDDKEVVMSLTEVKGYQLKGGIAIDGIEVRPK 280

BLAST of Tan0004461 vs. TAIR 10
Match: AT1G56250.1 (phloem protein 2-B14 )

HSP 1 Score: 228.4 bits (581), Expect = 7.4e-60
Identity = 127/288 (44.10%), Postives = 187/288 (64.93%), Query Frame = 0

Query: 9   MLPEDCVTTILSLTTPSDAGKLSLVSSTFRSAAESDVVWDRFLPENYAQIVAGSDISDEV 68
           MLPE C+  IL+ T+P+DA   S VSS FR A +SD VW++FLP +Y  +++ S      
Sbjct: 3   MLPEACIANILAFTSPADAFSSSEVSSVFRLAGDSDFVWEKFLPSDYKSLISQS-TDHHW 62

Query: 69  PFRSKRELFFRFCSAILIDGALNWKKM----GKISYILSARELDITWSSDPLCWSWKSHP 128
              SK+E++   C ++LID A    K+    GKISY+LSAR++ IT S     WSW +  
Sbjct: 63  NISSKKEIYRCLCDSLLIDNARKLFKINKFSGKISYVLSARDISITHSDHASYWSWSNVS 122

Query: 129 QSRFAEVAELRTVSWLEINGKIRTKSLSSNTLYGAYLLLKISERAYGLDLIAAQVSVQLG 188
            SRF+E AEL     LEI GKI+T+ LS+NT YGAYL++K+++ AYGLDL+ A+ S++  
Sbjct: 123 DSRFSESAELIITDRLEIEGKIQTRVLSANTRYGAYLIVKVTKGAYGLDLVPAETSIKSK 182

Query: 189 NQLQISQSSVWLQHKDKKNQDLECLFYGNRRQRLRNLMGSDKLLQNHQNESIKKDLKERQ 248
           N  QIS+S+ +L   D+K Q ++ LFYGNR +R+         ++    +  +++ K R 
Sbjct: 183 NG-QISKSATYLCCLDEKKQQMKRLFYGNREERMA------MTVEAVGGDGKRREPKCRD 242

Query: 249 DGWFEIELGQFFIAQNQNDEQVLITLMETKGFQLKSGLILEGIQIRPK 293
           DGW EIELG+F   + ++DE V +TL E KG+QLK G++++GI++RPK
Sbjct: 243 DGWMEIELGEFETREGEDDE-VNMTLTEVKGYQLKGGILIDGIEVRPK 281

BLAST of Tan0004461 vs. TAIR 10
Match: AT1G56240.1 (phloem protein 2-B13 )

HSP 1 Score: 224.2 bits (570), Expect = 1.4e-58
Identity = 126/287 (43.90%), Postives = 185/287 (64.46%), Query Frame = 0

Query: 9   MLPEDCVTTILSLTTPSDAGKLSLVSSTFRSAAESDVVWDRFLPENYAQIVAGSDISDEV 68
           MLPE CV  IL+ T+P+DA   S VSS FR A +SD VW++FLP +Y  +++ S     +
Sbjct: 3   MLPEACVANILAFTSPADAFSSSEVSSVFRLAGDSDFVWEKFLPSHYKSLISQSTDHHRI 62

Query: 69  PFRSKRELFFRFCSAILIDGALNWKKM----GKISYILSARELDITWSSDPLCWSWKSHP 128
            F SK+E++   C ++LID A    K+    GKISYILSAR++ IT+S      SW +  
Sbjct: 63  -FSSKKEIYRCLCDSLLIDNARKLFKINKFSGKISYILSARDISITYSDHASYCSWSNVS 122

Query: 129 QSRFAEVAELRTVSWLEINGKIRTKSLSSNTLYGAYLLLKISERAYGLDLIAAQVSVQLG 188
            SRF+E AEL T   LEI GKI+T  LS NT YGAYL++K++  AYGLDL+ A+ SV+  
Sbjct: 123 DSRFSESAELITTDRLEIKGKIQTTVLSPNTKYGAYLIMKVTNGAYGLDLVPAETSVKSK 182

Query: 189 NQLQISQSSVWLQHKDKKNQDLECLFYGNRRQRLRNLMGSDKLLQNHQNESIKKDLKERQ 248
           N  Q ++++ +L   D+K Q ++ LFYGNR +R+         ++    +  +++ K R 
Sbjct: 183 NG-QNNKNTTYLCCLDEKKQQMKRLFYGNREERMA------MTVEAVGGDGKRREPKARD 242

Query: 249 DGWFEIELGQFFIAQNQNDEQVLITLMETKGFQLKSGLILEGIQIRP 292
           DGW EIELG+F   + ++DE V ++L E KG+QLK G++++GI++RP
Sbjct: 243 DGWLEIELGEFVTREGEDDE-VNMSLTEVKGYQLKGGIVIDGIEVRP 280

BLAST of Tan0004461 vs. TAIR 10
Match: AT2G02240.1 (F-box family protein )

HSP 1 Score: 172.2 bits (435), Expect = 6.3e-43
Identity = 104/294 (35.37%), Postives = 156/294 (53.06%), Query Frame = 0

Query: 3   GILSIEMLPEDCVTTILSLTTPSDAGKLSLVSSTFRSAAESDVVWDRFLPENYAQIVAGS 62
           G    ++LPEDC++ I+S T+P DA   + VS TF SA  SD VWD+FLP  Y  +V+ S
Sbjct: 57  GTSPFDVLPEDCISNIISFTSPRDACVAASVSKTFESAVSSDCVWDKFLPPEYESLVSRS 116

Query: 63  DISDEVPFRSKRELFFRFC--SAILIDGALNW---KKMGKISYILSARELDITWSSDPLC 122
            +     F SK+EL+F  C    ++ DG  ++   K  GK   +LS++EL ITW S P  
Sbjct: 117 RV-----FASKKELYFALCHNPVLIEDGKKSFWLEKASGKRCIMLSSKELWITWGSSPEY 176

Query: 123 WSWKSHPQSRFAEVAELRTVSWLEINGKIRTKSLSSNTLYGAYLLLKISERAYGLDLIAA 182
           W W S P+SRF ++AEL  V W EI GK   + LS  T Y AY++ K  +R  GL  +  
Sbjct: 177 WQWISIPESRFNKIAELLDVCWFEIRGKTSARVLSPGTRYSAYIVFKTKDRCPGLGHLPV 236

Query: 183 QVSVQLGNQLQISQSSVWLQHKDKKNQDLECLFYGNRRQRLRNLMGSDKLLQNHQNESIK 242
           +V + L  Q    +   ++  +D++            R+  R++   D            
Sbjct: 237 EVGLGLVGQESSKRFIYFIGPRDRRG-----------RRETRDVTKPD------------ 296

Query: 243 KDLKERQDGWFEIELGQFFIAQNQNDEQVLITLMETKGFQLKSGLILEGIQIRP 292
               +R+DGW E ELG+FF    +  +++  +++E K    KSGLI++GI+ RP
Sbjct: 297 ----QREDGWMEAELGEFF--NEERCDEIEFSVIEIKSPSWKSGLIIQGIEFRP 316

BLAST of Tan0004461 vs. TAIR 10
Match: AT2G02230.1 (phloem protein 2-B1 )

HSP 1 Score: 167.9 bits (424), Expect = 1.2e-41
Identity = 105/310 (33.87%), Postives = 170/310 (54.84%), Query Frame = 0

Query: 8   EMLPEDCVTTILSLTTPSDAGKLSLVSSTFRSAAESDVVWDRFLPENYAQIVAGSDISDE 67
           + LPEDC++ ++S T+P DA  ++ VS + +SAA+SD+VW+ FLP  Y+ +V  S     
Sbjct: 33  DALPEDCISKVISHTSPRDACVVASVSKSVKSAAQSDLVWEMFLPSEYSSLVLQS----- 92

Query: 68  VPFRSKRELFFRFC-SAILIDGALN--W--KKMGKISYILSARELDITWSSDPLCWSWKS 127
               SK+E+F     +++L++      W  K  GK  Y+LSA EL I W   P  W W +
Sbjct: 93  ANHLSKKEIFLSLADNSVLVENGKKSFWVEKASGKKCYMLSAMELTIIWGDSPAYWKWIT 152

Query: 128 HPQSRFAEVAELRTVSWLEINGKIRTKSLSSNTLYGAYLLLKISE-RAYGLDLIAAQVSV 187
            P+S+F +VAELR V W E+ GKI    LS  T Y  Y++ K +  R+YG DL+  +  V
Sbjct: 153 VPESKFEKVAELRNVCWFEVRGKISCGMLSKGTHYSVYVVFKTANGRSYGFDLVPVEAGV 212

Query: 188 QLGNQLQISQSSVWLQHKDKKNQDLECLFYG------NRRQRLRNLMGSDKLLQNHQNES 247
               ++  ++ SV+ +  +  ++     + G      +R  R+R        +Q  + E 
Sbjct: 213 GFVGKV-ATKKSVYFESGNADSRSATSHYSGISYAMVSRAFRMRRPW---MQVQREEEEE 272

Query: 248 IKKDL---------KERQDGWFEIELGQFFI----AQNQNDEQVLITLMETKGFQLKSGL 293
           ++ +          KER DGW E+ELG+F+I      +   +++ I++MET+    KSGL
Sbjct: 273 VEGERERGMNVVGPKERVDGWSEVELGKFYINNGGCGDDGSDEIEISIMETQNGNWKSGL 332

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O804942.7e-6245.17F-box protein PP2-B15 OS=Arabidopsis thaliana OX=3702 GN=PP2B15 PE=2 SV=2[more]
Q9C7K01.0e-5844.10F-box protein VBF OS=Arabidopsis thaliana OX=3702 GN=VBF PE=1 SV=1[more]
Q9C7J92.0e-5743.90F-box protein PP2-B13 OS=Arabidopsis thaliana OX=3702 GN=PP2B13 PE=2 SV=1[more]
Q3E6P48.9e-4235.37F-box protein At2g02240 OS=Arabidopsis thaliana OX=3702 GN=At2g02240 PE=2 SV=1[more]
Q6NPT81.7e-4033.87F-box protein PP2-B1 OS=Arabidopsis thaliana OX=3702 GN=PP2B1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_022156423.11.2e-10969.57F-box protein VBF-like [Momordica charantia][more]
XP_023537654.18.4e-10666.89F-box protein PP2-B15-like [Cucurbita pepo subsp. pepo][more]
KAG7021074.11.4e-10567.45F-box protein PP2-B15, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022937763.12.4e-10567.22F-box protein PP2-B15-like [Cucurbita moschata] >KAG6586239.1 F-box protein PP2-... [more]
XP_022156417.13.2e-10568.56F-box protein VBF-like [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A6J1DQK96.0e-11069.57F-box protein VBF-like OS=Momordica charantia OX=3673 GN=LOC111023316 PE=4 SV=1[more]
A0A6J1FGV31.2e-10567.22F-box protein PP2-B15-like OS=Cucurbita moschata OX=3662 GN=LOC111444065 PE=4 SV... [more]
A0A6J1DS061.5e-10568.56F-box protein VBF-like OS=Momordica charantia OX=3673 GN=LOC111023312 PE=4 SV=1[more]
A0A6J1HPX53.2e-10366.89F-box protein PP2-B15-like OS=Cucurbita maxima OX=3661 GN=LOC111465617 PE=4 SV=1[more]
A0A6J1KF368.2e-9965.32F-box protein PP2-B15-like OS=Cucurbita maxima OX=3661 GN=LOC111493739 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G09155.11.9e-6345.17phloem protein 2-B15 [more]
AT1G56250.17.4e-6044.10phloem protein 2-B14 [more]
AT1G56240.11.4e-5843.90phloem protein 2-B13 [more]
AT2G02240.16.3e-4335.37F-box family protein [more]
AT2G02230.11.2e-4133.87phloem protein 2-B1 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001810F-box domainSMARTSM00256fbox_2coord: 10..50
e-value: 0.0012
score: 28.2
IPR001810F-box domainPFAMPF00646F-boxcoord: 7..50
e-value: 3.2E-5
score: 23.7
IPR001810F-box domainPROSITEPS50181FBOXcoord: 4..50
score: 10.318796
NoneNo IPR availableGENE3D1.20.1280.50coord: 3..111
e-value: 1.4E-5
score: 27.0
NoneNo IPR availablePANTHERPTHR32278FAMILY NOT NAMEDcoord: 8..292
NoneNo IPR availablePANTHERPTHR32278:SF15F-BOX PROTEIN PP2-B10-LIKEcoord: 8..292
IPR025886Phloem protein 2-likePFAMPF14299PP2coord: 98..291
e-value: 5.6E-46
score: 156.2
IPR036047F-box-like domain superfamilySUPERFAMILY81383F-box domaincoord: 4..81

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0004461.1Tan0004461.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0005515 protein binding