Tan0004342 (gene) Snake gourd v1

Overview
NameTan0004342
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionE3 ubiquitin-protein ligase RMA1H1-like
LocationLG05: 5662579 .. 5665276 (+)
RNA-Seq ExpressionTan0004342
SyntenyTan0004342
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CGTCATCGTCTCATCATCTACCGTTTTTGCTTTTGTTCCTCTTTCTATTTACAGAGAATTACAGATTCCTTCGCTCTGCCCTTTTATTCGCTCGCTCTCTTTCGCTCTTTATATAGACACCCTTGAATCCTCAACATCGCCTTCTTCAAATTCGCACCAATTCTTCCTCTCTTCTAACTCACCCCACCCGCTCATTCAACCTCTCTGAACTAAGCTTCTTACTCACACCCAGATAATAATCCGGCGATTAAGTTTCGGTACGTTTCGATTCGACCCCACTTTGTTTTCTCTCTGTGTATTGCTCTTTGATGGGTATGTCTTTCTCAGTGTGATTAACCTTTTTAGATCTGTTGCCAATGGAGATGGGGTTTTGTCAGTCTGTAATTACTTGGTTTCTTCGTAACCAGACGTTTAATTGGGTTCTTTGTTTTTGGGTTTTTTTTTTTCTTTATTGAACGCTTCTTTCATCGCTTCGAACTCCATCCCTGTTTTCGTGGATGAATTTTATAGGGTTTTGTTTGTTTTTTGGAAACGATTTTATTTGCTGTGCAGACGAGAAATTTTCATGCTCTGTTTTTGAGAGAGGTTTTGTGTTGTGGAGTGTGGGAATGGCTTCATTCCATTGTGTTGTTCGTGCCAAAGATACTATGCCAATATAGTGATGAATGACGAAGATTTTGTTTTCTGGATGTTCTTATTGGCTCTTTTGAAACAAAAGTTCACGATGGTTTTTCCAACTCTCTGAAAATTGAAGAGTGGTTACATCGATTCGGCATGGTCTTGTAGAGGAATTTTGATTTCTTTAGCTAGTATTGCAACTTGGGCTTGGATTCACTTGTATATTGCTTTCGGTTATGAATTGTTTGGATTATGGTTTATCTTTGTTTTGGTAAATTCTTAGTTTCATGTATGTCTTTTTTGTTTCTTCTAAGCATAGTTATGCCTCTCGGATCTGTTTTCCAAATAACGAACCTGAATTTATTTGTGTGCATAAAGATTGGTAGATCGTTTCACTTTTCAAGTGGTTTGCTTGCTTGCGCATGAGTTTATCTGCTTCAAAAGATAGCTTATGAACGCTGAAGATTCTGAATCTTCATCTTTCATCATGTTCCATGGTCCTCTGGAATTTTTAACCATTGTTTTAGCATAACATAATATAGGGCAGTTCTGGGATGGATTGTGTCTCTAAGAGATTTATTAATGAAACCCCATTACTTGAATTGCTTATAATTTAAAAAATTGAATCTGGGTGTGTCAAGAACAGCATGTTCTGATATTTATGACAACTTTAGAGATCATTTGCATCATTCTCCAGTTGGAGGTTTCGTGAAAGAACTCTTAAGAAGCGGCCACTGAAAATACAATAATCTATGAATGATGCTTACCCATATTCTTGTCAATTCTGGGACCCGATAAATAATATATTCTAACCAATAGATTAGGATGATTTGTTTGGCTGTTTCATCAGTTATCTTATCTCTACAATGGAAGGAATATATTTTGTGCATTGGTGAATTCCTTGTACGGCGTAGCTACAAAGCTTCATCTTATCTAAATTGCTGATTCTTTTGTCTGCTTCTGTGCATCTTTGTATTTTCAAACATCAGTCATTTTCTGTGATTTTCTGGTCAAGTTTTGCAAGAATTCATTGATCTTCTCTTGGAATCTAGTTGTATTCTTCTTGTTCTAAACTTGTTTTTTGGGCATGTTTCTCAGCTTGAGGCATTAGAAAAAGAAGACTGGTGAAACATAAACCTGGCCATGGAGCAGCAGTACCTTGATGAAACTTTGATGCGAAACAACAATTCATTAGGAGAAGACAAATTCTGTTTAGATAACCAAACATATGTATCAGATGATGGAGCTTCCAATGGCTTTGAATGCAACATATGCCTAGATTCAGTTCAAGATCCAGTTGTAACACTTTGTGGCCACCTCTTCTGTTGGCCCTGCATTTATAAATGGCTACATTACAAGACCTTATCTGCACAGCAACAATGCCAACAAGAGGAGTGTCGTTGTCCCGTTTGCAAGGCCGAAGTCTCCCATGCTTCTCTCGTCCCGCTTTACGGCAGGTTTCAGACTACAAATCCCTCCAAAACTAAAGCATCACATCTTGGCCCAGCCATCCCTCGCAGGCCTCTTGGCCCTGCCTGTGGAACAGAGACTCCCACATCGCCAAGTCCTCAACTTCACCCCCACAACTACTCTCCTCAGTCACACTCGTACTACCCACGAACACACGACTACATCGCTTCGTCGATGCTCAGCTCAAGCAGCATTACAACAAACATTATCCATCCAGTGATTGGAATGTTCGGTGAAGCGATTTACGCAAGAACATTCGGAAACACAACAACAAACCTCTATACCTATCCAAATTCATATGGTCATGTGAGCAACAGTAGTGCCAGGCTGAGGAGACACTTAATGCAAGCTGATGAGTCGCTGAGCAGAATCTGTTTCTTCCTTTTTTGTTGCATAGCATTGTGTCTTCTGTTATTCTAAGTAGCTTCCTTTAGATCATTTCTAGCCATTGGATTTGACATCCATTAGAATTTATGAACCAAGAGGGTTCAGCTCAGTTAGCTGTATATATATATATATTTATATATATACCTACAACATGTAATTTTGAGCTTAGAGTAAAAAAAAAAGGTAGAACTTTCCTTGAATCTGTATCGTGGAAGCTTCAAAT

mRNA sequence

CGTCATCGTCTCATCATCTACCGTTTTTGCTTTTGTTCCTCTTTCTATTTACAGAGAATTACAGATTCCTTCGCTCTGCCCTTTTATTCGCTCGCTCTCTTTCGCTCTTTATATAGACACCCTTGAATCCTCAACATCGCCTTCTTCAAATTCGCACCAATTCTTCCTCTCTTCTAACTCACCCCACCCGCTCATTCAACCTCTCTGAACTAAGCTTCTTACTCACACCCAGATAATAATCCGGCGATTAAGTTTCGCTTGAGGCATTAGAAAAAGAAGACTGGTGAAACATAAACCTGGCCATGGAGCAGCAGTACCTTGATGAAACTTTGATGCGAAACAACAATTCATTAGGAGAAGACAAATTCTGTTTAGATAACCAAACATATGTATCAGATGATGGAGCTTCCAATGGCTTTGAATGCAACATATGCCTAGATTCAGTTCAAGATCCAGTTGTAACACTTTGTGGCCACCTCTTCTGTTGGCCCTGCATTTATAAATGGCTACATTACAAGACCTTATCTGCACAGCAACAATGCCAACAAGAGGAGTGTCGTTGTCCCGTTTGCAAGGCCGAAGTCTCCCATGCTTCTCTCGTCCCGCTTTACGGCAGGTTTCAGACTACAAATCCCTCCAAAACTAAAGCATCACATCTTGGCCCAGCCATCCCTCGCAGGCCTCTTGGCCCTGCCTGTGGAACAGAGACTCCCACATCGCCAAGTCCTCAACTTCACCCCCACAACTACTCTCCTCAGTCACACTCGTACTACCCACGAACACACGACTACATCGCTTCGTCGATGCTCAGCTCAAGCAGCATTACAACAAACATTATCCATCCAGTGATTGGAATGTTCGGTGAAGCGATTTACGCAAGAACATTCGGAAACACAACAACAAACCTCTATACCTATCCAAATTCATATGGTCATGTGAGCAACAGTAGTGCCAGGCTGAGGAGACACTTAATGCAAGCTGATGAGTCGCTGAGCAGAATCTGTTTCTTCCTTTTTTGTTGCATAGCATTGTGTCTTCTGTTATTCTAAGTAGCTTCCTTTAGATCATTTCTAGCCATTGGATTTGACATCCATTAGAATTTATGAACCAAGAGGGTTCAGCTCAGTTAGCTGTATATATATATATATTTATATATATACCTACAACATGTAATTTTGAGCTTAGAGTAAAAAAAAAAGGTAGAACTTTCCTTGAATCTGTATCGTGGAAGCTTCAAAT

Coding sequence (CDS)

ATGGAGCAGCAGTACCTTGATGAAACTTTGATGCGAAACAACAATTCATTAGGAGAAGACAAATTCTGTTTAGATAACCAAACATATGTATCAGATGATGGAGCTTCCAATGGCTTTGAATGCAACATATGCCTAGATTCAGTTCAAGATCCAGTTGTAACACTTTGTGGCCACCTCTTCTGTTGGCCCTGCATTTATAAATGGCTACATTACAAGACCTTATCTGCACAGCAACAATGCCAACAAGAGGAGTGTCGTTGTCCCGTTTGCAAGGCCGAAGTCTCCCATGCTTCTCTCGTCCCGCTTTACGGCAGGTTTCAGACTACAAATCCCTCCAAAACTAAAGCATCACATCTTGGCCCAGCCATCCCTCGCAGGCCTCTTGGCCCTGCCTGTGGAACAGAGACTCCCACATCGCCAAGTCCTCAACTTCACCCCCACAACTACTCTCCTCAGTCACACTCGTACTACCCACGAACACACGACTACATCGCTTCGTCGATGCTCAGCTCAAGCAGCATTACAACAAACATTATCCATCCAGTGATTGGAATGTTCGGTGAAGCGATTTACGCAAGAACATTCGGAAACACAACAACAAACCTCTATACCTATCCAAATTCATATGGTCATGTGAGCAACAGTAGTGCCAGGCTGAGGAGACACTTAATGCAAGCTGATGAGTCGCTGAGCAGAATCTGTTTCTTCCTTTTTTGTTGCATAGCATTGTGTCTTCTGTTATTCTAA

Protein sequence

MEQQYLDETLMRNNNSLGEDKFCLDNQTYVSDDGASNGFECNICLDSVQDPVVTLCGHLFCWPCIYKWLHYKTLSAQQQCQQEECRCPVCKAEVSHASLVPLYGRFQTTNPSKTKASHLGPAIPRRPLGPACGTETPTSPSPQLHPHNYSPQSHSYYPRTHDYIASSMLSSSSITTNIIHPVIGMFGEAIYARTFGNTTTNLYTYPNSYGHVSNSSARLRRHLMQADESLSRICFFLFCCIALCLLLF
Homology
BLAST of Tan0004342 vs. ExPASy Swiss-Prot
Match: Q6R567 (E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum OX=4072 GN=RMA1H1 PE=1 SV=1)

HSP 1 Score: 224.6 bits (571), Expect = 1.3e-57
Identity = 123/255 (48.24%), Postives = 163/255 (63.92%), Query Frame = 0

Query: 1   MEQQYLDETLMRNNNSLGEDKFCLDNQTYVSDDGASNGFECNICLDSVQDPVVTLCGHLF 60
           M  + LD T  +++  LG+ K   D      ++  S GF+CNICLD V +PV+TLCGHL+
Sbjct: 5   MALEQLDTTFNKHDTPLGKWKSMNDE----VEENISGGFDCNICLDCVHEPVITLCGHLY 64

Query: 61  CWPCIYKWLHYKTLSAQQQCQQEECRCPVCKAEVSHASLVPLYGR-FQTTNPSKTKASHL 120
           CWPCIYKW++++++S++   QQ+  +CPVCKAEVS  +L+PLYGR  Q+T PS+ KA +L
Sbjct: 65  CWPCIYKWIYFQSVSSENSDQQQP-QCPVCKAEVSEKTLIPLYGRGGQSTKPSEGKAPNL 124

Query: 121 GPAIPRRPLGPACG-----TETPTSPSPQLHPHNYSPQSHSYYPRTH-DYIASSMLSSSS 180
           G  IP+RP  P CG       T ++PS  L    Y  QS +  P     Y++S MLS   
Sbjct: 125 GIVIPQRPPSPRCGGHFLLPTTDSNPSQLLQRRGYQQQSQTRQPAYQGSYMSSPMLSPGG 184

Query: 181 ITTNIIHPVIGMFGEAIYARTFGNTTTNLYTYPNSYGHVSNSSARLRRHLMQADESLSRI 240
            T N++     M GE  YAR FGN++T +YTYPNSY    +SS R+RR L QAD SL RI
Sbjct: 185 ATANMLQH--SMIGEVAYARIFGNSSTTMYTYPNSYNLAISSSPRMRRQLSQADRSLGRI 244

Query: 241 CFFLFCCIALCLLLF 249
           CFFLFCC   CL+LF
Sbjct: 245 CFFLFCCFVTCLILF 252

BLAST of Tan0004342 vs. ExPASy Swiss-Prot
Match: O64425 (E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis thaliana OX=3702 GN=RMA1 PE=1 SV=1)

HSP 1 Score: 191.4 bits (485), Expect = 1.2e-47
Identity = 108/256 (42.19%), Postives = 153/256 (59.77%), Query Frame = 0

Query: 1   MEQQYLDETLMRNNNSLGEDKFCLDNQ-------TYVSDDGASNGFECNICLDSVQDPVV 60
           ++Q + D  L+      GE  FC  ++       +  SDD   + F+CNICLDSVQ+PVV
Sbjct: 3   LDQSFEDAALL--GELYGEGAFCFKSKKPEPITVSVPSDDTDDSNFDCNICLDSVQEPVV 62

Query: 61  TLCGHLFCWPCIYKWLHYKTLSAQQQCQQEECRCPVCKAEVSHASLVPLYGRFQTTNPSK 120
           TLCGHLFCWPCI+KWL  ++ S   + Q+   +CPVCK++VSH++LVPLYGR + T   +
Sbjct: 63  TLCGHLFCWPCIHKWLDVQSFSTSDEYQRHR-QCPVCKSKVSHSTLVPLYGRGRCTTQEE 122

Query: 121 TKASHLGPAIPRRPLGPACGTETPTSPSPQLHPHNYSPQSHSYYPRTHDYIASSMLSSSS 180
            K S     +P+RP+GP    E P SP         S + H   P+   Y  S ++SS+S
Sbjct: 123 GKNS-----VPKRPVGPVYRLEMPNSPYAST-DLRLSQRVHFNSPQEGYYPVSGVMSSNS 182

Query: 181 IT-TNIIHPVIGMFGEAIYARTFGNTTTNLYTYPNSYGHVSNSSARLRRHLMQADESLSR 240
           ++ + ++ PV+ M GE +  R FG    + + YP++Y     S  R+RR +MQAD+SL R
Sbjct: 183 LSYSAVLDPVMVMVGEMVATRLFGTRVMDRFAYPDTYNLAGTSGPRMRRRIMQADKSLGR 242

Query: 241 ICFFLFCCIALCLLLF 249
           I FF  CC+ LCLLLF
Sbjct: 243 IFFFFMCCVVLCLLLF 249

BLAST of Tan0004342 vs. ExPASy Swiss-Prot
Match: Q8GUK7 (E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana OX=3702 GN=RMA3 PE=1 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 3.6e-36
Identity = 100/250 (40.00%), Postives = 132/250 (52.80%), Query Frame = 0

Query: 7   DETLMRNNNSLGEDKFCLDNQTYVSDDGASNGFECNICLDSVQDPVVTLCGHLFCWPCIY 66
           D     +N  + + K  L           S  F+CNICLD+  DPVVTLCGHLFCWPCIY
Sbjct: 10  DAQRAHDNGFIAKQKPNLTTAPTAGQANESGCFDCNICLDTAHDPVVTLCGHLFCWPCIY 69

Query: 67  KWLHYKTLSAQQQCQQEECRCPVCKAEVSHASLVPLYGRFQTTNPSKT----KASHLGPA 126
           KWLH +  S      Q +  CPVCK+ ++  SLVPLYGR   ++PS T    K   L   
Sbjct: 70  KWLHVQLSSV--SVDQHQNNCPVCKSNITITSLVPLYGR-GMSSPSSTFGSKKQDALSTD 129

Query: 127 IPRRPLGPACGT--ETPTSPSPQLHPHNYSP--QSHSYYPRTHDYIASSMLSSSSITTNI 186
           IPRRP   A      + +S +P L     SP   +H Y PR      S+ L ++++  + 
Sbjct: 130 IPRRPAPSALRNPITSASSLNPSLQHQTLSPSFHNHQYSPRGFTTTESTDL-ANAVMMSF 189

Query: 187 IHPVIGMFGEAIYARTFGNTTTNLYTYPNSYGHVSNSSARLRRHLMQADESLSRICFFLF 246
           ++PVIGMFG+ +Y R FG T TN    P              + +MQ ++SL+R+  F  
Sbjct: 190 LYPVIGMFGDLVYTRIFG-TFTNTIAQPYQ-----------SQRMMQREKSLNRVSIFFL 243

Query: 247 CCIALCLLLF 249
           CCI LCLLLF
Sbjct: 250 CCIILCLLLF 243

BLAST of Tan0004342 vs. ExPASy Swiss-Prot
Match: P93030 (E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis thaliana OX=3702 GN=RMA2 PE=1 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 1.3e-30
Identity = 89/228 (39.04%), Postives = 117/228 (51.32%), Query Frame = 0

Query: 25  DNQTYVSDDGASNGFECNICLDSVQDPVVTLCGHLFCWPCIYKWLHYKTLSAQQQCQ--- 84
           D+ T V   G    F+CNICLD V+DPVVTLCGHLFCWPCI+KW +    S Q+  Q   
Sbjct: 8   DDTTLVDSGG---DFDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDH 67

Query: 85  -QEECRCPVCKAEVSHASLVPLYGRFQTTNPSKTKASHLGPAIPRRPLGPACGTETPTSP 144
            +E  +CPVCK++VS A+LVP+YGR Q       KA   G  +P RP GP          
Sbjct: 68  KREPPKCPVCKSDVSEATLVPIYGRGQ-------KAPQSGSNVPSRPTGPVYDLR----- 127

Query: 145 SPQLHPHNYSPQSHSYYPRTHDYIASSMLSSSSITTNIIHPVIGMFGEAIYARTFGNTTT 204
              +       +S  Y  R  D                  PV+G+  E +Y R FG +++
Sbjct: 128 --GVGQRLGEGESQRYMYRMPD------------------PVMGVVCEMVYRRLFGESSS 187

Query: 205 NLYTYPNSYGHVSNSSARLRRHLMQADESLSRICFFLFCCIALCLLLF 249
           N+  Y        + + R RR  MQA+ESLSR+  FL C + +CL LF
Sbjct: 188 NMAPY-------RDMNVRSRRRAMQAEESLSRVYLFLLCFMFMCLFLF 193

BLAST of Tan0004342 vs. ExPASy Swiss-Prot
Match: Q5ZIR9 (E3 ubiquitin-protein ligase RNF185 OS=Gallus gallus OX=9031 GN=RNF185 PE=2 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 5.4e-16
Identity = 36/72 (50.00%), Postives = 48/72 (66.67%), Query Frame = 0

Query: 37  NGFECNICLDSVQDPVVTLCGHLFCWPCIYKWLHYKTLSAQQQCQQEECRCPVCKAEVSH 96
           N FECNICLD+ +D V++LCGHLFCWPC+++WL         + +     CPVCKA +S 
Sbjct: 37  NTFECNICLDTAKDAVISLCGHLFCWPCLHQWL---------ETRPNRQVCPVCKAGISR 96

Query: 97  ASLVPLYGRFQT 109
             ++PLYGR  T
Sbjct: 97  DKVIPLYGRGST 99

BLAST of Tan0004342 vs. NCBI nr
Match: XP_038901529.1 (E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida])

HSP 1 Score: 423.3 bits (1087), Expect = 1.4e-114
Identity = 208/252 (82.54%), Postives = 227/252 (90.08%), Query Frame = 0

Query: 1   MEQQYLDETLMRNNNSLGEDKFCLDNQTYVS--DDGASNGFECNICLDSVQDPVVTLCGH 60
           ME QY++ETL++NNNSL  +K CLDNQT +S  DDGAS+GF+CNICLDSVQDPVVTLCGH
Sbjct: 1   MEHQYVEETLIQNNNSLRGNKLCLDNQTCISDADDGASHGFDCNICLDSVQDPVVTLCGH 60

Query: 61  LFCWPCIYKWLHYKTLSAQQQCQQEECRCPVCKAEVSHASLVPLYGRFQTTNPSKTKA-S 120
           LFCWPCIYKWL YK LSAQQQCQQ ECRCPVCKAEVSH +LVP+YG+FQT NPSK KA S
Sbjct: 61  LFCWPCIYKWLQYKNLSAQQQCQQAECRCPVCKAEVSHTTLVPIYGKFQTINPSKAKAPS 120

Query: 121 HLGPAIPRRPLGPACGTETPTSPSPQLHPHNYSPQSHSYYPRT-HDYIASSMLSSSSITT 180
           +LGPAIPRRPLG ACGTE P SPSPQLHP NYSPQS+SYY RT ++YIASSMLSSSSITT
Sbjct: 121 NLGPAIPRRPLGRACGTEAPASPSPQLHPRNYSPQSNSYYSRTQNNYIASSMLSSSSITT 180

Query: 181 NIIHPVIGMFGEAIYARTFGNTTTNLYTYPNSYGHVSNSSARLRRHLMQADESLSRICFF 240
           NIIHPVIGMFG+AIY RTF NTT NLYTYPNSYGHVSNSS RLRRH+MQADESLSRICFF
Sbjct: 181 NIIHPVIGMFGDAIYTRTFRNTTPNLYTYPNSYGHVSNSSVRLRRHIMQADESLSRICFF 240

Query: 241 LFCCIALCLLLF 249
           LFCC+ +CLLLF
Sbjct: 241 LFCCLVICLLLF 252

BLAST of Tan0004342 vs. NCBI nr
Match: XP_022140292.1 (E3 ubiquitin-protein ligase RMA1H1-like [Momordica charantia] >XP_022140293.1 E3 ubiquitin-protein ligase RMA1H1-like [Momordica charantia])

HSP 1 Score: 420.6 bits (1080), Expect = 9.2e-114
Identity = 212/253 (83.79%), Postives = 225/253 (88.93%), Query Frame = 0

Query: 1   MEQQYLDETLMRNNNSLGEDKFCLDNQTYVS--DDGASNGFECNICLDSVQDPVVTLCGH 60
           MEQQYL+ET+MR +NS GEDKFCLDNQT VS  D GAS+GF+CNICLDSVQDPVVTLCGH
Sbjct: 1   MEQQYLEETVMR-SNSFGEDKFCLDNQTCVSDADGGASHGFDCNICLDSVQDPVVTLCGH 60

Query: 61  LFCWPCIYKWLHYKTLSAQQQCQQEECRCPVCKAEVSHASLVPLYGRFQTTNPSKTKASH 120
           LFCWPCIYKWLHYK LSAQQQCQ +E  CPVCKAEVSHASLVPLYGRFQTTNPS+TKAS 
Sbjct: 61  LFCWPCIYKWLHYKNLSAQQQCQPQENCCPVCKAEVSHASLVPLYGRFQTTNPSETKASQ 120

Query: 121 LGPAIPRRPLGPACG-TETPTSPSPQLHPHN-YSPQSHSYYPR-THDYIASSMLSSSSIT 180
           LGPAIPRRPLGPACG  E P SPSPQLHPHN Y P +HSY+PR T DYI SSML SSSIT
Sbjct: 121 LGPAIPRRPLGPACGAAEAPASPSPQLHPHNYYLPHAHSYHPRTTQDYITSSMLGSSSIT 180

Query: 181 TNIIHPVIGMFGEAIYARTFGNTTTNLYTYPNSYGHVSNSSARLRRHLMQADESLSRICF 240
           TNIIHPVIG FGEAIYAR FGNTTT+L+TYPNSYGHVSNSSARLRRH+MQADESLSRICF
Sbjct: 181 TNIIHPVIGTFGEAIYARAFGNTTTSLHTYPNSYGHVSNSSARLRRHMMQADESLSRICF 240

Query: 241 FLFCCIALCLLLF 249
           F FCC+ LC L F
Sbjct: 241 FFFCCLVLCFLSF 252

BLAST of Tan0004342 vs. NCBI nr
Match: XP_004139942.1 (E3 ubiquitin-protein ligase RMA1H1 [Cucumis sativus] >XP_031742415.1 E3 ubiquitin-protein ligase RMA1H1 [Cucumis sativus] >KGN46743.1 hypothetical protein Csa_020992 [Cucumis sativus])

HSP 1 Score: 397.5 bits (1020), Expect = 8.4e-107
Identity = 199/253 (78.66%), Postives = 225/253 (88.93%), Query Frame = 0

Query: 1   MEQQYLDETLMRNNNSLGEDKFCLDNQTYVSD--DGASNGFECNICLDSVQDPVVTLCGH 60
           MEQ+YL+ETLM+N+NSLG D+ CLDNQ Y+SD  D AS+GF+CNICLDSVQDPVVTLCGH
Sbjct: 1   MEQEYLEETLMQNDNSLGGDRLCLDNQIYISDAEDEASHGFDCNICLDSVQDPVVTLCGH 60

Query: 61  LFCWPCIYKWLHYKTLSAQQQCQQEECRCPVCKAEVSHASLVPLYGRFQTTNPSKTKA-S 120
           LFCWPCIYKWLH K LSA Q+CQQ ECRCPVCKA+VS A+LVP+YG+FQTT+ SK +A  
Sbjct: 61  LFCWPCIYKWLHCKKLSA-QRCQQVECRCPVCKAKVSRATLVPIYGKFQTTDASKAEAPP 120

Query: 121 HLGPAIPRRPLG-PACGTETPTSPSPQLHPHNYSPQSHSYYPRT-HDYIASSMLSSSSIT 180
           +LGPAIPRRPLG  AC  ETP SP+PQLH  NYSPQSHSYY +T ++YIASSMLSSSSIT
Sbjct: 121 NLGPAIPRRPLGRHACEAETPASPTPQLHSDNYSPQSHSYYAQTQNEYIASSMLSSSSIT 180

Query: 181 TNIIHPVIGMFGEAIYARTFGNTTTNLYTYPNSYGHVSNSSARLRRHLMQADESLSRICF 240
           TNIIHPVIGMFG+ IYARTFGNTTTNLYTYPNSYG+V+NSS RLRRH+MQADESLSRICF
Sbjct: 181 TNIIHPVIGMFGDTIYARTFGNTTTNLYTYPNSYGNVNNSSVRLRRHIMQADESLSRICF 240

Query: 241 FLFCCIALCLLLF 249
           F FCC+ +CLLLF
Sbjct: 241 FFFCCLVICLLLF 252

BLAST of Tan0004342 vs. NCBI nr
Match: XP_008448288.1 (PREDICTED: E3 ubiquitin-protein ligase RMA1H1 [Cucumis melo] >XP_008448289.1 PREDICTED: E3 ubiquitin-protein ligase RMA1H1 [Cucumis melo] >XP_016900554.1 PREDICTED: E3 ubiquitin-protein ligase RMA1H1 [Cucumis melo])

HSP 1 Score: 391.3 bits (1004), Expect = 6.0e-105
Identity = 198/253 (78.26%), Postives = 221/253 (87.35%), Query Frame = 0

Query: 1   MEQQYLDETLMRNNNSLGEDKFCLDNQTYVSD--DGASNGFECNICLDSVQDPVVTLCGH 60
           MEQQYL+E LM N+NSLG DK  LD QTY+SD  D AS+GF+CNICLDSVQDPVVTLCGH
Sbjct: 1   MEQQYLEEALMPNDNSLGGDKLRLDIQTYISDAEDEASHGFDCNICLDSVQDPVVTLCGH 60

Query: 61  LFCWPCIYKWLHYKTLSAQQQCQQEECRCPVCKAEVSHASLVPLYGRFQTTNPSKTKA-S 120
           LFCWPCIYKWLH K LSA QQCQQ ECRCPVCKA+VS A+L+P+YG+FQTT+ SK KA  
Sbjct: 61  LFCWPCIYKWLHSKKLSA-QQCQQVECRCPVCKAKVSRATLIPIYGKFQTTDASKAKAPP 120

Query: 121 HLGPAIPRRPLG-PACGTETPTSPSPQLHPHNYSPQSHSYYPRT-HDYIASSMLSSSSIT 180
           +LGPAIPRRPLG  AC  ETP SP+PQLH HNYSPQSHSYY +T ++YI+SSMLSSSSIT
Sbjct: 121 NLGPAIPRRPLGWHACEAETPASPTPQLHSHNYSPQSHSYYSQTQNEYISSSMLSSSSIT 180

Query: 181 TNIIHPVIGMFGEAIYARTFGNTTTNLYTYPNSYGHVSNSSARLRRHLMQADESLSRICF 240
           TNIIHPVIGMFG+ IY RT GNTTTNLY+YPNSYG+VSNSS RLRRH+MQADESLSRICF
Sbjct: 181 TNIIHPVIGMFGDTIYPRTLGNTTTNLYSYPNSYGNVSNSSVRLRRHIMQADESLSRICF 240

Query: 241 FLFCCIALCLLLF 249
           F FCC+ +CLLLF
Sbjct: 241 FFFCCLVICLLLF 252

BLAST of Tan0004342 vs. NCBI nr
Match: XP_023513341.1 (E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 380.6 bits (976), Expect = 1.1e-101
Identity = 186/219 (84.93%), Postives = 199/219 (90.87%), Query Frame = 0

Query: 31  SDDGASNGFECNICLDSVQDPVVTLCGHLFCWPCIYKWLHYKTLSAQQQCQQEECRCPVC 90
           +D+G S+GFECNICLDSVQDPVVTLCGHLFCWPCIYKW+HYK+LS+QQQCQQEECRCPVC
Sbjct: 10  ADEGVSHGFECNICLDSVQDPVVTLCGHLFCWPCIYKWIHYKSLSSQQQCQQEECRCPVC 69

Query: 91  KAEVSHASLVPLYGRFQTTNPSKTK-ASHLGPAIPRRPLGPACGTETPTSPSPQLHPHNY 150
           KAEVSHA+LVPLYG+FQTT PSKTK  S+LGPAIPRRPLGPACG E P SPS QLH HNY
Sbjct: 70  KAEVSHATLVPLYGKFQTTPPSKTKPPSNLGPAIPRRPLGPACGAEPPASPSAQLHHHNY 129

Query: 151 SPQSHSYYPRTHDYIASSMLSSSSITTNIIHPVIGMFGEAIYARTFGNTTTNLYTYPNSY 210
           S QSH       DYIASSMLSSSSITTNIIHPVIGMFG+A+Y RTFGNTTTNLYTYPNSY
Sbjct: 130 S-QSHD------DYIASSMLSSSSITTNIIHPVIGMFGDAVYTRTFGNTTTNLYTYPNSY 189

Query: 211 GHVSNSSARLRRHLMQADESLSRICFFLFCCIALCLLLF 249
           GHVSNSS RLRRH+MQADESLSRICFFLFCC+ LCLLLF
Sbjct: 190 GHVSNSSVRLRRHIMQADESLSRICFFLFCCLVLCLLLF 221

BLAST of Tan0004342 vs. ExPASy TrEMBL
Match: A0A6J1CHP4 (E3 ubiquitin-protein ligase RMA1H1-like OS=Momordica charantia OX=3673 GN=LOC111010997 PE=4 SV=1)

HSP 1 Score: 420.6 bits (1080), Expect = 4.5e-114
Identity = 212/253 (83.79%), Postives = 225/253 (88.93%), Query Frame = 0

Query: 1   MEQQYLDETLMRNNNSLGEDKFCLDNQTYVS--DDGASNGFECNICLDSVQDPVVTLCGH 60
           MEQQYL+ET+MR +NS GEDKFCLDNQT VS  D GAS+GF+CNICLDSVQDPVVTLCGH
Sbjct: 1   MEQQYLEETVMR-SNSFGEDKFCLDNQTCVSDADGGASHGFDCNICLDSVQDPVVTLCGH 60

Query: 61  LFCWPCIYKWLHYKTLSAQQQCQQEECRCPVCKAEVSHASLVPLYGRFQTTNPSKTKASH 120
           LFCWPCIYKWLHYK LSAQQQCQ +E  CPVCKAEVSHASLVPLYGRFQTTNPS+TKAS 
Sbjct: 61  LFCWPCIYKWLHYKNLSAQQQCQPQENCCPVCKAEVSHASLVPLYGRFQTTNPSETKASQ 120

Query: 121 LGPAIPRRPLGPACG-TETPTSPSPQLHPHN-YSPQSHSYYPR-THDYIASSMLSSSSIT 180
           LGPAIPRRPLGPACG  E P SPSPQLHPHN Y P +HSY+PR T DYI SSML SSSIT
Sbjct: 121 LGPAIPRRPLGPACGAAEAPASPSPQLHPHNYYLPHAHSYHPRTTQDYITSSMLGSSSIT 180

Query: 181 TNIIHPVIGMFGEAIYARTFGNTTTNLYTYPNSYGHVSNSSARLRRHLMQADESLSRICF 240
           TNIIHPVIG FGEAIYAR FGNTTT+L+TYPNSYGHVSNSSARLRRH+MQADESLSRICF
Sbjct: 181 TNIIHPVIGTFGEAIYARAFGNTTTSLHTYPNSYGHVSNSSARLRRHMMQADESLSRICF 240

Query: 241 FLFCCIALCLLLF 249
           F FCC+ LC L F
Sbjct: 241 FFFCCLVLCFLSF 252

BLAST of Tan0004342 vs. ExPASy TrEMBL
Match: A0A0A0KB39 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G128040 PE=4 SV=1)

HSP 1 Score: 397.5 bits (1020), Expect = 4.1e-107
Identity = 199/253 (78.66%), Postives = 225/253 (88.93%), Query Frame = 0

Query: 1   MEQQYLDETLMRNNNSLGEDKFCLDNQTYVSD--DGASNGFECNICLDSVQDPVVTLCGH 60
           MEQ+YL+ETLM+N+NSLG D+ CLDNQ Y+SD  D AS+GF+CNICLDSVQDPVVTLCGH
Sbjct: 1   MEQEYLEETLMQNDNSLGGDRLCLDNQIYISDAEDEASHGFDCNICLDSVQDPVVTLCGH 60

Query: 61  LFCWPCIYKWLHYKTLSAQQQCQQEECRCPVCKAEVSHASLVPLYGRFQTTNPSKTKA-S 120
           LFCWPCIYKWLH K LSA Q+CQQ ECRCPVCKA+VS A+LVP+YG+FQTT+ SK +A  
Sbjct: 61  LFCWPCIYKWLHCKKLSA-QRCQQVECRCPVCKAKVSRATLVPIYGKFQTTDASKAEAPP 120

Query: 121 HLGPAIPRRPLG-PACGTETPTSPSPQLHPHNYSPQSHSYYPRT-HDYIASSMLSSSSIT 180
           +LGPAIPRRPLG  AC  ETP SP+PQLH  NYSPQSHSYY +T ++YIASSMLSSSSIT
Sbjct: 121 NLGPAIPRRPLGRHACEAETPASPTPQLHSDNYSPQSHSYYAQTQNEYIASSMLSSSSIT 180

Query: 181 TNIIHPVIGMFGEAIYARTFGNTTTNLYTYPNSYGHVSNSSARLRRHLMQADESLSRICF 240
           TNIIHPVIGMFG+ IYARTFGNTTTNLYTYPNSYG+V+NSS RLRRH+MQADESLSRICF
Sbjct: 181 TNIIHPVIGMFGDTIYARTFGNTTTNLYTYPNSYGNVNNSSVRLRRHIMQADESLSRICF 240

Query: 241 FLFCCIALCLLLF 249
           F FCC+ +CLLLF
Sbjct: 241 FFFCCLVICLLLF 252

BLAST of Tan0004342 vs. ExPASy TrEMBL
Match: A0A1S3BJB6 (E3 ubiquitin-protein ligase RMA1H1 OS=Cucumis melo OX=3656 GN=LOC103490524 PE=4 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 2.9e-105
Identity = 198/253 (78.26%), Postives = 221/253 (87.35%), Query Frame = 0

Query: 1   MEQQYLDETLMRNNNSLGEDKFCLDNQTYVSD--DGASNGFECNICLDSVQDPVVTLCGH 60
           MEQQYL+E LM N+NSLG DK  LD QTY+SD  D AS+GF+CNICLDSVQDPVVTLCGH
Sbjct: 1   MEQQYLEEALMPNDNSLGGDKLRLDIQTYISDAEDEASHGFDCNICLDSVQDPVVTLCGH 60

Query: 61  LFCWPCIYKWLHYKTLSAQQQCQQEECRCPVCKAEVSHASLVPLYGRFQTTNPSKTKA-S 120
           LFCWPCIYKWLH K LSA QQCQQ ECRCPVCKA+VS A+L+P+YG+FQTT+ SK KA  
Sbjct: 61  LFCWPCIYKWLHSKKLSA-QQCQQVECRCPVCKAKVSRATLIPIYGKFQTTDASKAKAPP 120

Query: 121 HLGPAIPRRPLG-PACGTETPTSPSPQLHPHNYSPQSHSYYPRT-HDYIASSMLSSSSIT 180
           +LGPAIPRRPLG  AC  ETP SP+PQLH HNYSPQSHSYY +T ++YI+SSMLSSSSIT
Sbjct: 121 NLGPAIPRRPLGWHACEAETPASPTPQLHSHNYSPQSHSYYSQTQNEYISSSMLSSSSIT 180

Query: 181 TNIIHPVIGMFGEAIYARTFGNTTTNLYTYPNSYGHVSNSSARLRRHLMQADESLSRICF 240
           TNIIHPVIGMFG+ IY RT GNTTTNLY+YPNSYG+VSNSS RLRRH+MQADESLSRICF
Sbjct: 181 TNIIHPVIGMFGDTIYPRTLGNTTTNLYSYPNSYGNVSNSSVRLRRHIMQADESLSRICF 240

Query: 241 FLFCCIALCLLLF 249
           F FCC+ +CLLLF
Sbjct: 241 FFFCCLVICLLLF 252

BLAST of Tan0004342 vs. ExPASy TrEMBL
Match: A0A6J1FXR1 (E3 ubiquitin-protein ligase RMA1H1-like OS=Cucurbita moschata OX=3662 GN=LOC111448157 PE=4 SV=1)

HSP 1 Score: 378.3 bits (970), Expect = 2.5e-101
Identity = 185/219 (84.47%), Postives = 199/219 (90.87%), Query Frame = 0

Query: 31  SDDGASNGFECNICLDSVQDPVVTLCGHLFCWPCIYKWLHYKTLSAQQQCQQEECRCPVC 90
           +D+G S+GFECNICLDSVQDPVVTLCGHLFCWPCIYKW+HYK+LS+QQQCQQEECRCPVC
Sbjct: 10  ADEGVSHGFECNICLDSVQDPVVTLCGHLFCWPCIYKWIHYKSLSSQQQCQQEECRCPVC 69

Query: 91  KAEVSHASLVPLYGRFQTTNPSKTK-ASHLGPAIPRRPLGPACGTETPTSPSPQLHPHNY 150
           KAEVSHA+LVPLYG+FQTT PSKTK  S+LGPAIPRRPLGPACG E P SPS QLH HNY
Sbjct: 70  KAEVSHATLVPLYGKFQTTPPSKTKPPSNLGPAIPRRPLGPACGGEPPASPSAQLHHHNY 129

Query: 151 SPQSHSYYPRTHDYIASSMLSSSSITTNIIHPVIGMFGEAIYARTFGNTTTNLYTYPNSY 210
           S QSH       DYIASSMLSSSSITTNIIHPVIGMFG+A+Y RTFGNTT+NLYTYPNSY
Sbjct: 130 S-QSHD------DYIASSMLSSSSITTNIIHPVIGMFGDAVYTRTFGNTTSNLYTYPNSY 189

Query: 211 GHVSNSSARLRRHLMQADESLSRICFFLFCCIALCLLLF 249
           GHVSNSS RLRRH+MQADESLSRICFFLFCC+ LCLLLF
Sbjct: 190 GHVSNSSVRLRRHIMQADESLSRICFFLFCCLVLCLLLF 221

BLAST of Tan0004342 vs. ExPASy TrEMBL
Match: A0A6J1J9X8 (E3 ubiquitin-protein ligase RMA1H1-like OS=Cucurbita maxima OX=3661 GN=LOC111483889 PE=4 SV=1)

HSP 1 Score: 372.5 bits (955), Expect = 1.4e-99
Identity = 182/217 (83.87%), Postives = 196/217 (90.32%), Query Frame = 0

Query: 31  SDDGASNGFECNICLDSVQDPVVTLCGHLFCWPCIYKWLHYKTLSAQQQCQQEECRCPVC 90
           +D+G S+GFECNICLDSVQDPVVTLCGHLFCWPCIYKW+HYK+LS+QQQCQQEECRCPVC
Sbjct: 10  ADEGVSHGFECNICLDSVQDPVVTLCGHLFCWPCIYKWIHYKSLSSQQQCQQEECRCPVC 69

Query: 91  KAEVSHASLVPLYGRFQTTNPSKTK-ASHLGPAIPRRPLGPACGTETPTSPSPQLHPHNY 150
           KAEVSHA+LVPLYG+FQTT PSKTK  S+LGPAIPRRPLGPACG E P SPS QLH HNY
Sbjct: 70  KAEVSHATLVPLYGKFQTTAPSKTKPPSNLGPAIPRRPLGPACGAEPPASPSSQLHHHNY 129

Query: 151 SPQSHSYYPRTHDYIASSMLSSSSITTNIIHPVIGMFGEAIYARTFGNTTTNLYTYPNSY 210
           S QSH      +DYIASSMLSSSSITTNIIHPVIGMFG+A+Y RTFGNTTTNLYTYPNSY
Sbjct: 130 S-QSH------NDYIASSMLSSSSITTNIIHPVIGMFGDAVYTRTFGNTTTNLYTYPNSY 189

Query: 211 GHVSNSSARLRRHLMQADESLSRICFFLFCCIALCLL 247
           GHVSNSS RLRRH+MQADESLSRICFFLFCC+   LL
Sbjct: 190 GHVSNSSVRLRRHIMQADESLSRICFFLFCCLVFLLL 219

BLAST of Tan0004342 vs. TAIR 10
Match: AT4G03510.1 (RING membrane-anchor 1 )

HSP 1 Score: 191.4 bits (485), Expect = 8.5e-49
Identity = 108/256 (42.19%), Postives = 153/256 (59.77%), Query Frame = 0

Query: 1   MEQQYLDETLMRNNNSLGEDKFCLDNQ-------TYVSDDGASNGFECNICLDSVQDPVV 60
           ++Q + D  L+      GE  FC  ++       +  SDD   + F+CNICLDSVQ+PVV
Sbjct: 3   LDQSFEDAALL--GELYGEGAFCFKSKKPEPITVSVPSDDTDDSNFDCNICLDSVQEPVV 62

Query: 61  TLCGHLFCWPCIYKWLHYKTLSAQQQCQQEECRCPVCKAEVSHASLVPLYGRFQTTNPSK 120
           TLCGHLFCWPCI+KWL  ++ S   + Q+   +CPVCK++VSH++LVPLYGR + T   +
Sbjct: 63  TLCGHLFCWPCIHKWLDVQSFSTSDEYQRHR-QCPVCKSKVSHSTLVPLYGRGRCTTQEE 122

Query: 121 TKASHLGPAIPRRPLGPACGTETPTSPSPQLHPHNYSPQSHSYYPRTHDYIASSMLSSSS 180
            K S     +P+RP+GP    E P SP         S + H   P+   Y  S ++SS+S
Sbjct: 123 GKNS-----VPKRPVGPVYRLEMPNSPYAST-DLRLSQRVHFNSPQEGYYPVSGVMSSNS 182

Query: 181 IT-TNIIHPVIGMFGEAIYARTFGNTTTNLYTYPNSYGHVSNSSARLRRHLMQADESLSR 240
           ++ + ++ PV+ M GE +  R FG    + + YP++Y     S  R+RR +MQAD+SL R
Sbjct: 183 LSYSAVLDPVMVMVGEMVATRLFGTRVMDRFAYPDTYNLAGTSGPRMRRRIMQADKSLGR 242

Query: 241 ICFFLFCCIALCLLLF 249
           I FF  CC+ LCLLLF
Sbjct: 243 IFFFFMCCVVLCLLLF 249

BLAST of Tan0004342 vs. TAIR 10
Match: AT4G03510.2 (RING membrane-anchor 1 )

HSP 1 Score: 191.4 bits (485), Expect = 8.5e-49
Identity = 108/256 (42.19%), Postives = 153/256 (59.77%), Query Frame = 0

Query: 1   MEQQYLDETLMRNNNSLGEDKFCLDNQ-------TYVSDDGASNGFECNICLDSVQDPVV 60
           ++Q + D  L+      GE  FC  ++       +  SDD   + F+CNICLDSVQ+PVV
Sbjct: 3   LDQSFEDAALL--GELYGEGAFCFKSKKPEPITVSVPSDDTDDSNFDCNICLDSVQEPVV 62

Query: 61  TLCGHLFCWPCIYKWLHYKTLSAQQQCQQEECRCPVCKAEVSHASLVPLYGRFQTTNPSK 120
           TLCGHLFCWPCI+KWL  ++ S   + Q+   +CPVCK++VSH++LVPLYGR + T   +
Sbjct: 63  TLCGHLFCWPCIHKWLDVQSFSTSDEYQRHR-QCPVCKSKVSHSTLVPLYGRGRCTTQEE 122

Query: 121 TKASHLGPAIPRRPLGPACGTETPTSPSPQLHPHNYSPQSHSYYPRTHDYIASSMLSSSS 180
            K S     +P+RP+GP    E P SP         S + H   P+   Y  S ++SS+S
Sbjct: 123 GKNS-----VPKRPVGPVYRLEMPNSPYAST-DLRLSQRVHFNSPQEGYYPVSGVMSSNS 182

Query: 181 IT-TNIIHPVIGMFGEAIYARTFGNTTTNLYTYPNSYGHVSNSSARLRRHLMQADESLSR 240
           ++ + ++ PV+ M GE +  R FG    + + YP++Y     S  R+RR +MQAD+SL R
Sbjct: 183 LSYSAVLDPVMVMVGEMVATRLFGTRVMDRFAYPDTYNLAGTSGPRMRRRIMQADKSLGR 242

Query: 241 ICFFLFCCIALCLLLF 249
           I FF  CC+ LCLLLF
Sbjct: 243 IFFFFMCCVVLCLLLF 249

BLAST of Tan0004342 vs. TAIR 10
Match: AT4G27470.1 (RING membrane-anchor 3 )

HSP 1 Score: 153.3 bits (386), Expect = 2.6e-37
Identity = 100/250 (40.00%), Postives = 132/250 (52.80%), Query Frame = 0

Query: 7   DETLMRNNNSLGEDKFCLDNQTYVSDDGASNGFECNICLDSVQDPVVTLCGHLFCWPCIY 66
           D     +N  + + K  L           S  F+CNICLD+  DPVVTLCGHLFCWPCIY
Sbjct: 10  DAQRAHDNGFIAKQKPNLTTAPTAGQANESGCFDCNICLDTAHDPVVTLCGHLFCWPCIY 69

Query: 67  KWLHYKTLSAQQQCQQEECRCPVCKAEVSHASLVPLYGRFQTTNPSKT----KASHLGPA 126
           KWLH +  S      Q +  CPVCK+ ++  SLVPLYGR   ++PS T    K   L   
Sbjct: 70  KWLHVQLSSV--SVDQHQNNCPVCKSNITITSLVPLYGR-GMSSPSSTFGSKKQDALSTD 129

Query: 127 IPRRPLGPACGT--ETPTSPSPQLHPHNYSP--QSHSYYPRTHDYIASSMLSSSSITTNI 186
           IPRRP   A      + +S +P L     SP   +H Y PR      S+ L ++++  + 
Sbjct: 130 IPRRPAPSALRNPITSASSLNPSLQHQTLSPSFHNHQYSPRGFTTTESTDL-ANAVMMSF 189

Query: 187 IHPVIGMFGEAIYARTFGNTTTNLYTYPNSYGHVSNSSARLRRHLMQADESLSRICFFLF 246
           ++PVIGMFG+ +Y R FG T TN    P              + +MQ ++SL+R+  F  
Sbjct: 190 LYPVIGMFGDLVYTRIFG-TFTNTIAQPYQ-----------SQRMMQREKSLNRVSIFFL 243

Query: 247 CCIALCLLLF 249
           CCI LCLLLF
Sbjct: 250 CCIILCLLLF 243

BLAST of Tan0004342 vs. TAIR 10
Match: AT4G28270.1 (RING membrane-anchor 2 )

HSP 1 Score: 134.8 bits (338), Expect = 9.4e-32
Identity = 89/228 (39.04%), Postives = 117/228 (51.32%), Query Frame = 0

Query: 25  DNQTYVSDDGASNGFECNICLDSVQDPVVTLCGHLFCWPCIYKWLHYKTLSAQQQCQ--- 84
           D+ T V   G    F+CNICLD V+DPVVTLCGHLFCWPCI+KW +    S Q+  Q   
Sbjct: 8   DDTTLVDSGG---DFDCNICLDQVRDPVVTLCGHLFCWPCIHKWTYASNNSRQRVDQYDH 67

Query: 85  -QEECRCPVCKAEVSHASLVPLYGRFQTTNPSKTKASHLGPAIPRRPLGPACGTETPTSP 144
            +E  +CPVCK++VS A+LVP+YGR Q       KA   G  +P RP GP          
Sbjct: 68  KREPPKCPVCKSDVSEATLVPIYGRGQ-------KAPQSGSNVPSRPTGPVYDLR----- 127

Query: 145 SPQLHPHNYSPQSHSYYPRTHDYIASSMLSSSSITTNIIHPVIGMFGEAIYARTFGNTTT 204
              +       +S  Y  R  D                  PV+G+  E +Y R FG +++
Sbjct: 128 --GVGQRLGEGESQRYMYRMPD------------------PVMGVVCEMVYRRLFGESSS 187

Query: 205 NLYTYPNSYGHVSNSSARLRRHLMQADESLSRICFFLFCCIALCLLLF 249
           N+  Y        + + R RR  MQA+ESLSR+  FL C + +CL LF
Sbjct: 188 NMAPY-------RDMNVRSRRRAMQAEESLSRVYLFLLCFMFMCLFLF 193

BLAST of Tan0004342 vs. TAIR 10
Match: AT1G19310.1 (RING/U-box superfamily protein )

HSP 1 Score: 107.5 bits (267), Expect = 1.6e-23
Identity = 54/118 (45.76%), Postives = 70/118 (59.32%), Query Frame = 0

Query: 31  SDDGASNGFECNICLDSVQDPVVTLCGHLFCWPCIYKWLHYKTLSAQQQCQQEECRCPVC 90
           +++  S+ FECNICLD  QDP+VTLCGHLFCWPC+YKWLH  + S           CPVC
Sbjct: 13  TNNNDSSNFECNICLDLAQDPIVTLCGHLFCWPCLYKWLHLHSQSKD---------CPVC 72

Query: 91  KAEVSHASLVPLYGRFQTTNPSKTKASHLGPAIPRRPLGPACGTETPTSPSPQLHPHN 149
           KA +    LVPLYGR +++   ++K S  G  +P RP G    T  P  P+     H+
Sbjct: 73  KAVIEEDRLVPLYGRGKSSADPRSK-SIPGLEVPNRPSGQRPETAQPPDPNHGFAHHH 120

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q6R5671.3e-5748.24E3 ubiquitin-protein ligase RMA1H1 OS=Capsicum annuum OX=4072 GN=RMA1H1 PE=1 SV=... [more]
O644251.2e-4742.19E3 ubiquitin-protein ligase RMA1 OS=Arabidopsis thaliana OX=3702 GN=RMA1 PE=1 SV... [more]
Q8GUK73.6e-3640.00E3 ubiquitin-protein ligase RMA3 OS=Arabidopsis thaliana OX=3702 GN=RMA3 PE=1 SV... [more]
P930301.3e-3039.04E3 ubiquitin-protein ligase RMA2 OS=Arabidopsis thaliana OX=3702 GN=RMA2 PE=1 SV... [more]
Q5ZIR95.4e-1650.00E3 ubiquitin-protein ligase RNF185 OS=Gallus gallus OX=9031 GN=RNF185 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
XP_038901529.11.4e-11482.54E3 ubiquitin-protein ligase RMA1H1-like [Benincasa hispida][more]
XP_022140292.19.2e-11483.79E3 ubiquitin-protein ligase RMA1H1-like [Momordica charantia] >XP_022140293.1 E3... [more]
XP_004139942.18.4e-10778.66E3 ubiquitin-protein ligase RMA1H1 [Cucumis sativus] >XP_031742415.1 E3 ubiquiti... [more]
XP_008448288.16.0e-10578.26PREDICTED: E3 ubiquitin-protein ligase RMA1H1 [Cucumis melo] >XP_008448289.1 PRE... [more]
XP_023513341.11.1e-10184.93E3 ubiquitin-protein ligase RMA1H1-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
A0A6J1CHP44.5e-11483.79E3 ubiquitin-protein ligase RMA1H1-like OS=Momordica charantia OX=3673 GN=LOC111... [more]
A0A0A0KB394.1e-10778.66RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G128040 P... [more]
A0A1S3BJB62.9e-10578.26E3 ubiquitin-protein ligase RMA1H1 OS=Cucumis melo OX=3656 GN=LOC103490524 PE=4 ... [more]
A0A6J1FXR12.5e-10184.47E3 ubiquitin-protein ligase RMA1H1-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1J9X81.4e-9983.87E3 ubiquitin-protein ligase RMA1H1-like OS=Cucurbita maxima OX=3661 GN=LOC111483... [more]
Match NameE-valueIdentityDescription
AT4G03510.18.5e-4942.19RING membrane-anchor 1 [more]
AT4G03510.28.5e-4942.19RING membrane-anchor 1 [more]
AT4G27470.12.6e-3740.00RING membrane-anchor 3 [more]
AT4G28270.19.4e-3239.04RING membrane-anchor 2 [more]
AT1G19310.11.6e-2345.76RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 41..90
e-value: 2.2E-8
score: 43.8
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 41..91
score: 12.858103
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 16..121
e-value: 3.4E-17
score: 64.3
IPR027370RING-type zinc-finger, LisH dimerisation motifPFAMPF13445zf-RING_UBOXcoord: 41..88
e-value: 8.6E-8
score: 32.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 112..147
NoneNo IPR availablePANTHERPTHR12313:SF75SUBFAMILY NOT NAMEDcoord: 1..248
NoneNo IPR availableCDDcd16745RING-HC_AtRMA_likecoord: 39..92
e-value: 4.84594E-23
score: 86.204
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 36..103
IPR045103E3 ubiquitin-protein ligase RNF5/RNF185-likePANTHERPTHR12313E3 UBIQUITIN-PROTEIN LIGASE RNF5-RELATEDcoord: 1..248
IPR017907Zinc finger, RING-type, conserved sitePROSITEPS00518ZF_RING_1coord: 56..65

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0004342.1Tan0004342.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071712 ER-associated misfolded protein catabolic process
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0046872 metal ion binding
molecular_function GO:0044390 ubiquitin-like protein conjugating enzyme binding
molecular_function GO:0061630 ubiquitin protein ligase activity