Tan0004158 (gene) Snake gourd v1

BLAST of Tan0004158 vs. ExPASy Swiss-Prot
Match: Q9LYG9 (Mechanosensitive ion channel protein 10 OS=Arabidopsis thaliana OX=3702 GN=MSL10 PE=1 SV=1)

HSP 1 Score: 570.1 bits (1468), Expect = 3.7e-161
Identity = 345/694 (49.71%), Postives = 459/694 (66.14%), Query Frame = 0

Query: 55  SPSHEIVRM--SPLKPPK-------------------ISMPKSY---PAC---ASLAEEK 114
           SPS EI ++  SP KPP+                    S PKS    P+C    S+ EE+
Sbjct: 46  SPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEEE 105

Query: 115 AQAKSTSGSLFCSSPKITTVTSPRDALQSAPRTPKGSGGEEDDNEEDYKTAEQKVKERSR 174
            + +  +G  F  S       S R     AP TP     E+D++EE YK  + K+    R
Sbjct: 106 VREQLGAG--FSFSRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYK--KVKLNREMR 165

Query: 175 KKSKIPILIELGAFLCITGCLIASLTIDKLLTKEIWGLGLWRWCVLILVIFSGRLFSQWF 234
            K     LIE   F+ I   L+ASLTI+ L     WGL +W+WCVL++VIFSG L + WF
Sbjct: 166 SKISTLALIESAFFVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWF 225

Query: 235 IKCLVFLFERNFIFQEKVLYFIYGLRKSVIFFIWLGLILLAWVLLFNQSSDRSKKDKEIL 294
           ++ +VFL E NF+ + KVLYF++GL+KSV  FIWL LIL+AW+LLFN    RS    ++L
Sbjct: 226 MRLIVFLIETNFLLRRKVLYFVHGLKKSVQVFIWLCLILVAWILLFNHDVKRSPAATKVL 285

Query: 295 NYITRALGGSIIGATLWLVKTLLVKIVAASFQCAKFFERIKESFFHNYILQTLSRPPLMT 354
             ITR L   + GA  WLVKTLL+KI+AA+F    FF+RI++S FH Y+LQTLS  PLM 
Sbjct: 286 KCITRTLISILTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLME 345

Query: 355 MTERVRRVASTGHLCYFKFLKKERDGKKEGKKGGIDVEKLRKMKQEEISAWTMSGLIDDI 414
             ERV R  STGHL +   +KK   G  + KK  ID+ K+ KMK+E++SAWTM  L++ +
Sbjct: 346 EAERVGREPSTGHLSFATVVKK---GTVKEKK-VIDMGKVHKMKREKVSAWTMRVLMEAV 405

Query: 415 RNSGLSTISDTI-ENFNEEEAEQKDKEIKNEWEAKAAANQIFRNVAKAGSKFIEEEDLFP 474
           R SGLSTISDT+ E    E  EQ D+EI +E EA AAA  +FRNVA+    +IEEEDL  
Sbjct: 406 RTSGLSTISDTLDETAYGEGKEQADREITSEMEALAAAYHVFRNVAQPFFNYIEEEDLLR 465

Query: 475 FMRKEVVNNVLKLFEGAVENRRIKRKTLKNWLVKVYQERKSLAQSLNDTKTAIEELNRLA 534
           FM KE V+ V  LF+GA E  RI RK    W+VKVY  R++LA SLNDTKTA+++LN+L 
Sbjct: 466 FMIKEEVDLVFPLFDGAAETGRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLV 525

Query: 535 SAFVLFVAIIVWLLVMGFLTMKVLVFISSQLLLVAFMFGNTAKTVFEAIIFVFVMHPFDV 594
           +A ++ V +++WLL++   T KVL+F S+QL+ +AF+ G+T K +FE+I+FVFVMHP+DV
Sbjct: 526 TAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDV 585

Query: 595 GDRCVVDGVQMIVEEMNLLTTVFLKYDNEMIIYPNSVLATKPISNFYRSPPKMIDLVEFT 654
           GDRCVVDGV M+VEEMNLLTTVFLK +NE + YPN+VLATKPISN++RS P M + VEF+
Sbjct: 586 GDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRS-PNMGETVEFS 645

Query: 655 VDFSTSIKSIEDLKAGIKSYIAKNPKIWSSNHSFLVKDIENVDKMKMALYVNHTMNFQEY 714
           + FST +  I  LK  I  Y+ +NP+ W+  HS +VK+IEN++K+KMALY +HT+ FQE 
Sbjct: 646 ISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQEN 705

Query: 715 TLKSNRRSELVLELKRIFENLGIKYHLLPQQVQL 721
             ++ RR+EL L +KR+ E+L I Y LLPQ + L
Sbjct: 706 RERNLRRTELSLAIKRMLEDLHIDYTLLPQDINL 730

BLAST of Tan0004158 vs. ExPASy Swiss-Prot
Match: Q84M97 (Mechanosensitive ion channel protein 9 OS=Arabidopsis thaliana OX=3702 GN=MSL9 PE=1 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 4.1e-144
Identity = 330/753 (43.82%), Postives = 461/753 (61.22%), Query Frame = 0

Query: 8   EQVVVPILDSEGIVESSEERSVASFPDLEINDT----QSFRCTIPLSVVETSPSHEIVRM 67
           E+VV+ + D E   +S + R+  SF  L   D+    +  +   P+S+    P+ EI + 
Sbjct: 10  EEVVINVSDKE---DSKDPRASPSFNPLASPDSDAGIEKSKPVPPISI----PTPEIYKF 69

Query: 68  SPL--KPPKI-----------------SMPKS---------YPAC------ASLAEEKAQ 127
           S    KPPKI                 S PKS         Y +        SL E+   
Sbjct: 70  SGSVHKPPKIPSPEGLVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLREQFGA 129

Query: 128 AKSTSGSLFCSSPKITTVTSPRDALQSAPRTPKGSGGEEDDNEEDYKTAEQKVKERSRKK 187
                GS   +SP   +  S   A  S     K +  E D+NEE YK    KVK    K+
Sbjct: 130 GSFARGSFDRASPNNKSNRSVASAALS-----KVAEEEPDENEEIYK----KVKLHRVKR 189

Query: 188 SKIPIL--IELGAFLCITGCLIASLTIDKLLTKEIWGLGLWRWCVLILVIFSGRLFSQWF 247
           S +  L  +EL  F+ I G LI SLTID +    IWGL  W+WCVL++V  SG L + WF
Sbjct: 190 SGMKPLAFLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLVTNWF 249

Query: 248 IKCLVFLFERNFIFQEKVLYFIYGLRKSVIFFIWLGLILLAWVLLFNQSSDRSKKDKEIL 307
           +  +VF+ E+N++ ++KVLYF++GL+K+V  FIW  L+L+AW+ LF+    R++K K  L
Sbjct: 250 MHFVVFIIEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGDVKRTRKTKRFL 309

Query: 308 NYITRALGGSIIGATLWLVKTLLVKIVAASFQCAKFFERIKESFFHNYILQTLSRPPLMT 367
           ++IT  +   ++G+ L+LVKT  +K++A+ F    FFERI+ES FH Y+LQTLS PPL+ 
Sbjct: 310 DFITWTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSGPPLIE 369

Query: 368 MTERVRRVASTGHLCYFKFLKKERDGKKEGKKGGIDVEKLRKMKQEEISAWTMSGLIDDI 427
             E V RV STGHL +     + +DGK + KK  ID+ K+ +MKQE++SAWTM  LI+ +
Sbjct: 370 EAENVGRVPSTGHLSF----TRTKDGKVKDKK-VIDMGKVHRMKQEKVSAWTMRVLIEAV 429

Query: 428 RNSGLSTISDTIENFNEEEAEQKDKEIKNEWEAKAAANQIFRNVAKAGSKFIEEEDLFPF 487
             SG+STIS T++  N ++ E+ DKEI NE EA AAA  +F NVAK    +IEE+DL  F
Sbjct: 430 GTSGISTISSTLDEVNNKK-ERTDKEITNEMEAVAAAYDVFNNVAKPNHNYIEEDDLLRF 489

Query: 488 MRKEVVNNVLKLFEGAVENRRIKRKTLKNWLVKVYQERKSLAQSLNDTKTAIEELNRLAS 547
           M KE V+ VL L E A +  +I RKT   W+V VY  RK++  SLNDTKTA+++L++L +
Sbjct: 490 MIKEEVDLVLPLIEDA-DTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVKQLDKLIT 549

Query: 548 AFVLFVAIIVWLLVMGFLTMKVLVFISSQLLLVAFMFGNTAKTVFEAIIFVFVMHPFDVG 607
             +  +  IVW++++   + K+L+  SSQ L +AFM G+T K +FE+ +FVFVMHP+DVG
Sbjct: 550 GILTVITFIVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHPYDVG 609

Query: 608 DRCVVDGVQMIVEEMNLLTTVFLKYDNEMIIYPNSVLATKPISNFYRSPPKMIDLVEFTV 667
           DRCVVDGV ++VEE++LLTTVFLK DNE + YPNSVL +KPISNFYRS P M D V+F +
Sbjct: 610 DRCVVDGVMLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRS-PDMGDYVDFGI 669

Query: 668 DFSTSIKSIEDLKAGIKSYIAKNPKIWSSNHSFLVKDIENVDKMKMALYVNHTMNFQEYT 721
            FST  + I  LK  I  Y+  N + W      +V+ IEN++K+ + + V HT+NFQ Y 
Sbjct: 670 AFSTPAEKIGCLKGKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTINFQVYV 729

BLAST of Tan0004158 vs. ExPASy Swiss-Prot
Match: Q9LH74 (Mechanosensitive ion channel protein 5 OS=Arabidopsis thaliana OX=3702 GN=MSL5 PE=2 SV=1)

HSP 1 Score: 453.8 bits (1166), Expect = 3.8e-126
Identity = 267/636 (41.98%), Postives = 399/636 (62.74%), Query Frame = 0

Query: 123 GSGGEEDDNEEDYKTAEQKVKERSRKKSKIPILIELGAFLCITGCLIASLTIDKLLTKEI 182
           G  G E++ EED    E   +E  R K    + +E  + + I   L+ SLTI  L  K  
Sbjct: 238 GRNGFEEEEEEDPFLDEDLPEEFKRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTW 297

Query: 183 WGLGLWRWCVLILVIFSGRLFSQWFIKCLVFLFERNFIFQEKVLYFIYGLRKSVIFFIWL 242
           W L LW+W V +LV+  GRL S W ++ +VFL E+NF ++++VLYF+YG+RKSV   +WL
Sbjct: 298 WKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWL 357

Query: 243 GLILLAWVLLFNQSSDRSKKDKEILNYITRALGGSIIGATLWLVKTLLVKIVAASFQCAK 302
           GL+LLAW  LF++  +R  +    L Y+TR L   ++   +WLVKT+LVK++A+SF  + 
Sbjct: 358 GLVLLAWHFLFDKKVERETR-STALRYVTRVLVCLLVALIIWLVKTILVKVLASSFHMST 417

Query: 303 FFERIKESFFHNYILQTLSRPPLM---TMTERVRRVA----STGHLCYFK---------- 362
           +F+RI+ES F  Y+++TLS PPLM    M E  ++VA    S   L   K          
Sbjct: 418 YFDRIQESLFTQYVIETLSGPPLMEIQRMEEEEQQVAEDVKSLEKLAGAKLPPALKATVK 477

Query: 363 -FLK------KERDGKKEGKKG-GIDVEKLRKMKQEEISAWTMSGLIDDIRNSGLSTISD 422
            F+K        R G K G+ G GI +++L++M  + +SAW M  L++ I    +ST+  
Sbjct: 478 SFMKVGKSPGLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQ 537

Query: 423 TIENFNEEEAEQKDKEIKNEWEAKAAANQIFRNVAKAGSKFIEEEDLFPFMRKEVVNNVL 482
            +++  +E+ +     I++E+EAK AA +IF NV + GS++I  ED   F+ +E     +
Sbjct: 538 NMQDTTQEDEDA--THIRSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAM 597

Query: 483 KLFEGAVENRRIKRKTLKNWLVKVYQERKSLAQSLNDTKTAIEELNRLASAFVLFVAIIV 542
            LFEGA E+ +I +  LKNW+VK ++ER++LA +LNDTKTA++ L+R+ +  +  + II+
Sbjct: 598 ALFEGASESDKISKSCLKNWVVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIII 657

Query: 543 WLLVMGFLTMKVLVFISSQLLLVAFMFGNTAKTVFEAIIFVFVMHPFDVGDRCVVDGVQM 602
           WLL++G  T + L+ +SSQLLLVAF+FGN+ KT+FEAIIF+FVMHPFDVGDRC +DGVQ+
Sbjct: 658 WLLILGIATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQL 717

Query: 603 IVEEMNLLTTVFLKYDNEMIIYPNSVLATKPISNFYRSPPKMIDLVEFTVDFSTSIKSIE 662
           +VEEMN+LTTVFL+YDN+ IIYPNSVL TKPI+N+YRS P M D VEF V  +T  + I 
Sbjct: 718 VVEEMNILTTVFLRYDNQKIIYPNSVLGTKPIANYYRS-PDMGDAVEFCVHIATPPEKIT 777

Query: 663 DLKAGIKSYIAKNPKIWSSNHSFLVKDIENVDKMKMALYVNHTMNFQEYTLKSNRRSELV 722
            +K  I SY+      W      +   +++++ +K+A+++ H MN Q+   +  RR  L+
Sbjct: 778 AIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLL 837

Query: 723 LELKRIFENLGIKYHLLPQQVQLNYGSSPALPPSRN 734
            E+ +    L I+Y L P  + +      +LPP+ N
Sbjct: 838 EEVGKTCRELDIEYRLYPLNINVR-----SLPPTAN 864

BLAST of Tan0004158 vs. ExPASy Swiss-Prot
Match: Q9LPG3 (Mechanosensitive ion channel protein 4 OS=Arabidopsis thaliana OX=3702 GN=MSL4 PE=3 SV=1)

HSP 1 Score: 448.4 bits (1152), Expect = 1.6e-124
Identity = 264/648 (40.74%), Postives = 398/648 (61.42%), Query Frame = 0

Query: 118 PRTPKGSGGEEDDNEEDYKTAEQKVKERSRKKSKIPILIELGAFLCITGCLIASLTIDKL 177
           P TP   G ++ + EED  + E   +   ++K  + ++IE    + I   LI SL I  L
Sbjct: 223 PGTPNQGGSKDLEEEEDPFSEEDLPEGLRKEKICVWVIIEWIFLILIIASLICSLVIPYL 282

Query: 178 LTKEIWGLGLWRWCVLILVIFSGRLFSQWFIKCLVFLFERNFIFQEKVLYFIYGLRKSVI 237
             K +W L LW+W V++LV+  GRL S W +K  V+  E NF++++KVLYF+YG+RK V 
Sbjct: 283 RGKTLWDLALWKWEVMVLVLICGRLVSSWIVKLFVYFVESNFLWRKKVLYFVYGIRKPVQ 342

Query: 238 FFIWLGLILLAWVLLFNQSSDRSKKDKEILNYITRALGGSIIGATLWLVKTLLVKIVAAS 297
             +WLGL+L+AW  LF++  +R  +   +L Y+T+ L   ++   +WL+KTLLVK++A+S
Sbjct: 343 NCLWLGLVLIAWHFLFDKKVEREMR-STVLKYVTKVLICLLVAVIIWLIKTLLVKVLASS 402

Query: 298 FQCAKFFERIKESFFHNYILQTLSRPPLMTM---TERVRRVASTGHLCYFKF-------- 357
           F  + +F+RI+ES F  Y+++TLS PP + +    E+V     T  +   K         
Sbjct: 403 FHMSTYFDRIQESLFTQYVIETLSGPPRIEIHIEEEKVANDVKTFEIVGRKLSPLGPKAV 462

Query: 358 ------------LKK-----------ERDG-KKEGKKGGIDVEKLRKMKQEEISAWTMSG 417
                       L+K            R G KKEG + GI ++ L++M  + +SAW M  
Sbjct: 463 SSPPQVTVGSGRLQKSPSRVGKSPVLSRSGSKKEGGEEGIRIDHLQRMNTKNVSAWKMKK 522

Query: 418 LIDDIRNSGLSTISDTIENFNEEEAEQKDKEIKNEWEAKAAANQIFRNVAKAGSKFIEEE 477
           L++ I+   LST+ + I++   +E + K  +I++E+EAK AA +IF+NVA+ GS++I  E
Sbjct: 523 LMNVIKKGTLSTLDEQIQDTTTQE-DDKATQIRSEFEAKLAARKIFQNVAEPGSRYIYME 582

Query: 478 DLFPFMRKEVVNNVLKLFEGAVENRRIKRKTLKNWLVKVYQERKSLAQSLNDTKTAIEEL 537
           D   F+ ++     + LFEGA E  +I +  LKNW+V  ++ER++LA +LNDTKTA+  L
Sbjct: 583 DFMRFLSEDESERAMDLFEGASECHKISKSCLKNWVVNAFRERRALALTLNDTKTAVNRL 642

Query: 538 NRLASAFVLFVAIIVWLLVMGFLTMKVLVFISSQLLLVAFMFGNTAKTVFEAIIFVFVMH 597
           +R+    V  V +I+WLL++G  T K L+ ISSQLLLV F+FGN+ KT+FEA+IFVFVMH
Sbjct: 643 HRIVDVLVSIVILIIWLLILGIATTKFLLVISSQLLLVVFVFGNSCKTIFEAVIFVFVMH 702

Query: 598 PFDVGDRCVVDGVQMIVEEMNLLTTVFLKYDNEMIIYPNSVLATKPISNFYRSPPKMIDL 657
           PFDVGDRC +DGVQMIVEEMN+LTTVFL++DN+ I+YPNS+L TKPI+N+YRS P M D 
Sbjct: 703 PFDVGDRCEIDGVQMIVEEMNILTTVFLRFDNQKIVYPNSLLGTKPIANYYRS-PDMQDA 762

Query: 658 VEFTVDFSTSIKSIEDLKAGIKSYIAKNPKIWSSNHSFLVKDIENVDKMKMALYVNHTMN 717
           +EF V  +T  +    L+  I SY+      W  +   + +D+  ++ +K+A++  H MN
Sbjct: 763 IEFFVHIATPPEKTTALRQRILSYVDNKKDHWHPSPMIVFRDMCGLNSVKIAMWPTHKMN 822

Query: 718 FQEYTLKSNRRSELVLELKRIFENLGIKYHLLPQQVQLNYGSSPALPP 731
            Q    +  RR +L+ E+ R+   L I+Y L P  + +N  S PA  P
Sbjct: 823 HQNMGERYVRRGQLLEEIGRLCRELDIEYRLYP--LNINVKSLPAATP 865

BLAST of Tan0004158 vs. ExPASy Swiss-Prot
Match: Q9SYM1 (Mechanosensitive ion channel protein 6 OS=Arabidopsis thaliana OX=3702 GN=MSL6 PE=1 SV=1)

HSP 1 Score: 439.5 bits (1129), Expect = 7.5e-122
Identity = 256/639 (40.06%), Postives = 401/639 (62.75%), Query Frame = 0

Query: 119 RTPKGSGGEEDDNEEDYKTAEQKVKERSRKKSKIPILIELGAFLCITGCLIASLTIDKLL 178
           ++PK  G EE+D   D   AE   +E  + K  + I++E  + + I    + +L I  L 
Sbjct: 210 KSPKTQGEEEED---DPFAAEDLPEEYRKDKLSLWIVLEWLSLILIIAGFVCTLAIPSLR 269

Query: 179 TKEIWGLGLWRWCVLILVIFSGRLFSQWFIKCLVFLFERNFIFQEKVLYFIYGLRKSVIF 238
            K++W L LW+W  ++LV+  GRL S W +K +VF  ERNF+ +++VLYF+YG+RK+V  
Sbjct: 270 KKKLWELQLWKWESMVLVLICGRLVSSWIVKIVVFFIERNFLLRKRVLYFVYGVRKAVQN 329

Query: 239 FIWLGLILLAWVLLFNQSSDRSKKDKEILNYITRALGGSIIGATLWLVKTLLVKIVAASF 298
            +WLGL+LLAW  LF++   ++   K  L  +T+     ++G  LWLVKTLLVK++A+SF
Sbjct: 330 CLWLGLVLLAWHFLFDEKVAKAANTK-ALRVVTKIFVCLLVGFLLWLVKTLLVKVLASSF 389

Query: 299 QCAKFFERIKESFFHNYILQTLSRPPLMTMTE----------RVRRVASTGHLCYFKFLK 358
             + +F+RI+ES F  Y+++TLS PPL+ + +           V++  + G +      +
Sbjct: 390 HMSTYFDRIQESLFTQYVIETLSGPPLIEIQKNEEEEERISVEVKKFQNPGGVEIQSGAQ 449

Query: 359 KE--RDGKKE-------------GKKGGIDVEKLRKMKQEEISAWTMSGLIDDIRNSGLS 418
           K   + GK               G+  GI ++ L K+  + +SAW M  L++ IRN  L+
Sbjct: 450 KSPMKTGKSPFLSHVLSNGGGGGGENKGITIDSLHKLNPKNVSAWKMKRLMNIIRNGSLT 509

Query: 419 TISDTIENFNEEEAEQKDKEIKNEWEAKAAANQIFRNVAKAGSKFIEEEDLFPFMRKEVV 478
           T+ + +++ + +  + K  +I++E+EAK AA +IF NVAK GSKFI   D+  F+  +  
Sbjct: 510 TLDEQLQDPSLD--DDKGNQIRSEFEAKLAARKIFHNVAKPGSKFIYANDIMRFLPDDEA 569

Query: 479 NNVLKLFEGAVENRRIKRKTLKNWLVKVYQERKSLAQSLNDTKTAIEELNRLASAFVLFV 538
              L LFEGA E  RI + +LKNW+V  ++ER++LA +LNDTKTA+  L+++ +  V  +
Sbjct: 570 LKTLSLFEGASETNRISKSSLKNWVVNAFRERRALALTLNDTKTAVNRLHKMVNIVVGII 629

Query: 539 AIIVWLLVMGFLTMKVLVFISSQLLLVAFMFGNTAKTVFEAIIFVFVMHPFDVGDRCVVD 598
            +++WL+++G  + K LV +SSQ+++VAF+FGN  K VFE+II++FV+HPFDVGDRC +D
Sbjct: 630 ILVIWLIILGITSTKFLVVMSSQVVVVAFIFGNMCKIVFESIIYLFVIHPFDVGDRCEID 689

Query: 599 GVQMIVEEMNLLTTVFLKYDNEMIIYPNSVLATKPISNFYRSPPKMIDLVEFTVDFSTSI 658
           GVQM+VEEMN+LTTVFL++DN+ ++YPNS+L TK I N+YRS P M D +EF++  +T  
Sbjct: 690 GVQMVVEEMNILTTVFLRFDNQKVVYPNSLLWTKSIGNYYRS-PDMGDGIEFSIHITTPA 749

Query: 659 KSIEDLKAGIKSYIAKNPKIWSSNHSFLVKDIENVDKMKMALYVNHTMNFQEYTLKSNRR 718
           + I  +K  I SYI      W      + KD+E+++ +++A++  H MN Q+   K  RR
Sbjct: 750 EKIILIKQRITSYIEGKKDHWYPAPMIVFKDMESLNSVRIAVWPTHRMNHQDMGEKWARR 809

Query: 719 SELVLELKRIFENLGIKYHLLPQQVQL-NYGSSPALPPS 732
           S+LV E+ +I   L I+Y L P  + + N  +S ALP S
Sbjct: 810 SQLVEEIAKICRELDIEYRLYPLDINVRNLPTSTALPVS 841

BLAST of Tan0004158 vs. NCBI nr
Match: XP_022987285.1 (mechanosensitive ion channel protein 10-like [Cucurbita maxima] >XP_022987286.1 mechanosensitive ion channel protein 10-like [Cucurbita maxima])

HSP 1 Score: 970.3 bits (2507), Expect = 9.3e-279
Identity = 543/763 (71.17%), Postives = 615/763 (80.60%), Query Frame = 0

Query: 1   MADKKGVEQVVVPILDSEGIVESSE---ERSVASFPDLEINDTQSFRCTIPLSVVETSPS 60
           MADKKG EQVV+ ILD E  V++       SVASFPD E  +T+S RC IP SVVE+SPS
Sbjct: 1   MADKKGTEQVVLRILDGEEGVDARNGLTNGSVASFPDFESKETRSVRCAIPQSVVESSPS 60

Query: 61  HEIVRMSPLKPPKI----------------SMPKS---YPAC---ASLAEEKAQAKSTSG 120
           HEI RMS LKPPKI                S PKS    P C   AS AEE AQAKSTSG
Sbjct: 61  HEISRMSSLKPPKIPVESAVRRPSFARSSFSKPKSRLIEPPCPDGASSAEENAQAKSTSG 120

Query: 121 SLFCSSP------KITTVTSPRDALQSAPRTPK-----GSGGEEDDNEEDYKTAEQKVKE 180
           S F SSP      KI T TSP+++L+SAP TP+      +G EE+D+EE YKTAE KVKE
Sbjct: 121 SPFSSSPKMDSPAKIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180

Query: 181 RSRKKSKIPILIELGAFLCITGCLIASLTIDKLLTKEIWGLGLWRWCVLILVIFSGRLFS 240
           +SRKK K  +LIE  AFLCIT CLI+SLTIDKLLTKEIWGLGLW+WCVL+LVIF GRLFS
Sbjct: 181 KSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFS 240

Query: 241 QWFIKCLVFLFERNFIFQEKVLYFIYGLRKSVIFFIWLGLILLAWVLLFNQSSDRSKKDK 300
           QWFI CLVFL ERNF+ + KVLYF+YGLRKSVI FIWL L+LLAW LLF+QS  RSKK  
Sbjct: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLALVLLAWGLLFDQSIKRSKKVN 300

Query: 301 EILNYITRALGGSIIGATLWLVKTLLVKIVAASFQCAKFFERIKESFFHNYILQTLSRPP 360
           EILNY+TRAL  S+IGA LWLVKTLLVKI+AASFQC +FF+RI+ES FH YIL+ LS PP
Sbjct: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPP 360

Query: 361 LMTMTERVRRVASTGHLCYFKFLKKERDGKKEGKKGGIDVEKLRKMKQEEISAWTMSGLI 420
           LM M ERV RVAS G L  F+ LKKERDG  EGK+  IDV+KL+KMKQ ++SAWTM GLI
Sbjct: 361 LMEMAERVGRVASAGQLS-FRHLKKERDGGNEGKEEVIDVDKLKKMKQAKVSAWTMRGLI 420

Query: 421 DDIRNSGLSTISDTIENFNEEEAEQKDKEIKNEWEAKAAANQIFRNVAKAGSKFIEEEDL 480
           + IR+SGLSTIS+TIENF EEE EQKDKEI +EWEA AAA QIFRNVAK GSK+I+EEDL
Sbjct: 421 NVIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDL 480

Query: 481 FPFMRKEVVNNVLKLFEGAVENRRIKRKTLKNWLVKVYQERKSLAQSLNDTKTAIEELNR 540
           F FM KE ++NVL LFEG VE  +IKRKTLKNWLV VY ERKSLA SLNDTKTAIEELNR
Sbjct: 481 FRFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNR 540

Query: 541 LASAFVLFVAIIVWLLVMGFLTMKVLVFISSQLLLVAFMFGNTAKTVFEAIIFVFVMHPF 600
           LASAFVL V IIVWLL+MGFLT +VLVFISSQLLLV FMFGNTAKT+FEAIIFVFVMHPF
Sbjct: 541 LASAFVLIVIIIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPF 600

Query: 601 DVGDRCVVDGVQMIVEEMNLLTTVFLKYDNEMIIYPNSVLATKPISNFYRSPPKMIDLVE 660
           DVGDRCVVDGVQMIVEEMN+LTT+FL+YDNE I YPNSVLATKPISNFYRS P+M D ++
Sbjct: 601 DVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRS-PEMSDSID 660

Query: 661 FTVDFSTSIKSIEDLKAGIKSYIAKNPKIWSSNHSFLVKDIENVDKMKMALYVNHTMNFQ 720
           F+VDFSTSI+SI  LKA IKSY+   P+ W  NHS +VK+IENV+KMK++LY+NHT+NFQ
Sbjct: 661 FSVDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQ 720

Query: 721 EYTLKSNRRSELVLELKRIFENLGIKYHLLPQQVQLNYGSSPA 728
            Y  +S+RRS+LVLELK+IFE+LGIKYHLLPQ+VQLNY SS A
Sbjct: 721 NYGDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSIA 761

BLAST of Tan0004158 vs. NCBI nr
Match: KAG7023687.1 (Mechanosensitive ion channel protein 10, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 968.4 bits (2502), Expect = 3.5e-278
Identity = 540/758 (71.24%), Postives = 614/758 (81.00%), Query Frame = 0

Query: 1   MADKKGVEQVVVPILDSEGIVESSEE---RSVASFPDLEINDTQSFRCTIPLSVVETSPS 60
           MADKKG EQVV+ ILD E  V++  +    SVAS PD E  +T+S RC IP SVVE+SPS
Sbjct: 1   MADKKGTEQVVLRILDGEEGVDARNDLTNGSVASVPDFEPKETRSVRCAIPQSVVESSPS 60

Query: 61  HEIVRMSPLKPPKI----------------SMPKS---YPAC---ASLAEEKAQAKSTSG 120
           HEI RMS LKPPKI                S PKS    P C   ASLAEE AQAKSTSG
Sbjct: 61  HEISRMSSLKPPKIPVGSAVRRPSFARSSFSKPKSRLIEPPCPDGASLAEENAQAKSTSG 120

Query: 121 SLFCSSPK------ITTVTSPRDALQSAPRTPK-----GSGGEEDDNEEDYKTAEQKVKE 180
           S F SSPK      I T TSP+++L+SAP TP+      +G EE+D+EE YKTAE KVKE
Sbjct: 121 SPFSSSPKMDSPANIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180

Query: 181 RSRKKSKIPILIELGAFLCITGCLIASLTIDKLLTKEIWGLGLWRWCVLILVIFSGRLFS 240
           +SRKK K  +LIE  AFLCIT CLI+SLTIDKLLTKEIWGLGLW+WCVL+LVIF GRLFS
Sbjct: 181 KSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFS 240

Query: 241 QWFIKCLVFLFERNFIFQEKVLYFIYGLRKSVIFFIWLGLILLAWVLLFNQSSDRSKKDK 300
           QWFI CLVFL ERNF+ + KVLYF+YGLRKSVI FIWL L+LLAW LLF+QS  RSKK  
Sbjct: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLALVLLAWGLLFDQSIKRSKKVN 300

Query: 301 EILNYITRALGGSIIGATLWLVKTLLVKIVAASFQCAKFFERIKESFFHNYILQTLSRPP 360
           EILNY+TRAL  S+IGA LWLVKTLLVKI+AASFQC +FF+RI+ES FH YIL+TLS PP
Sbjct: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360

Query: 361 LMTMTERVRRVASTGHLCYFKFLKKERDGKKEGKKGGIDVEKLRKMKQEEISAWTMSGLI 420
           LM M ERV   AS+G L  F+ LKKERDG  EGK+  IDV+KL+KMKQE++SAWTM GLI
Sbjct: 361 LMEMAERVGGAASSGQLS-FRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLI 420

Query: 421 DDIRNSGLSTISDTIENFNEEEAEQKDKEIKNEWEAKAAANQIFRNVAKAGSKFIEEEDL 480
           + IR+SGLSTIS+TIENF EEE EQKDKEI +EWEA AAA QIFRNVAK GSK+I+EEDL
Sbjct: 421 NVIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDL 480

Query: 481 FPFMRKEVVNNVLKLFEGAVENRRIKRKTLKNWLVKVYQERKSLAQSLNDTKTAIEELNR 540
           F FM KE ++NVL LFEG VE  +IKRKTLKNWLV VY ERKSLA SLNDTKTAIEELNR
Sbjct: 481 FRFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNR 540

Query: 541 LASAFVLFVAIIVWLLVMGFLTMKVLVFISSQLLLVAFMFGNTAKTVFEAIIFVFVMHPF 600
           LASAFVL V IIVWLL+MGFLT +VLVFISSQLLLV FMFGNTAKT+FEAIIFVFVMHPF
Sbjct: 541 LASAFVLIVIIIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPF 600

Query: 601 DVGDRCVVDGVQMIVEEMNLLTTVFLKYDNEMIIYPNSVLATKPISNFYRSPPKMIDLVE 660
           DVGDRCVVDGVQMIVEEMN+LTT+FL+YDNE I YPNSVLATKPISNFYRS P+M D ++
Sbjct: 601 DVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRS-PEMSDSID 660

Query: 661 FTVDFSTSIKSIEDLKAGIKSYIAKNPKIWSSNHSFLVKDIENVDKMKMALYVNHTMNFQ 720
           F+VDFSTSI+SI  LKA IKSY+   P+ W  NHS +VK+IENV+KMK++LY+NHT+NFQ
Sbjct: 661 FSVDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQ 720

Query: 721 EYTLKSNRRSELVLELKRIFENLGIKYHLLPQQVQLNY 723
            Y  +S+RRS+LVLELK+IFE+LGIKYHLLPQ+VQLNY
Sbjct: 721 NYGDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNY 756

BLAST of Tan0004158 vs. NCBI nr
Match: XP_038878911.1 (mechanosensitive ion channel protein 10-like [Benincasa hispida])

HSP 1 Score: 966.1 bits (2496), Expect = 1.7e-277
Identity = 537/764 (70.29%), Postives = 624/764 (81.68%), Query Frame = 0

Query: 1   MADKKGVEQVVVPILDSEGIVESSEE---RSVASFPDLEINDTQSFRCTIPLSVVETSPS 60
           MADKKG+EQ+V+ IL+ E  V++S++    SV+SFPD ++ +T+SFRCTIP SVV +SPS
Sbjct: 1   MADKKGMEQLVLRILEGEEGVDTSKDLTKPSVSSFPDFDLKETRSFRCTIPQSVVGSSPS 60

Query: 61  HEIVRMSPLKPPKI----------------SMPKS------YPACASLAEEKAQAKST-- 120
           HEI RMSPLKPPKI                S PKS       P  ASLAEEKA+AKST  
Sbjct: 61  HEISRMSPLKPPKIPGETAIRRPSFARSSFSKPKSRLIESPCPDSASLAEEKAKAKSTLY 120

Query: 121 SGSLFCSSPKITTVTSPRDALQSAPRTPK-----GSGGEEDDNEEDYKTAEQKVKERSRK 180
           S     S  KITTVTSP++AL+SAP TPK      +G EE+D+EE YKTAE KVKERS K
Sbjct: 121 SSPKMDSPAKITTVTSPKEALKSAPITPKTPLIGTTGSEEEDDEEVYKTAELKVKERSGK 180

Query: 181 KSKIPILIELGAFLCITGCLIASLTIDKLLTKEIWGLGLWRWCVLILVIFSGRLFSQWFI 240
           K K  +LIE  AFLC+TGCLIASLTIDKL+TKEIWGLGLW+WCVL+LV F GRLFSQWFI
Sbjct: 181 KLKRTVLIEWVAFLCLTGCLIASLTIDKLVTKEIWGLGLWKWCVLVLVSFCGRLFSQWFI 240

Query: 241 KCLVFLFERNFIFQEKVLYFIYGLRKSVIFFIWLGLILLAWVLLFNQSSDRSKKDKEILN 300
            CLVFL ERNF+ + KVLYF+YGLRKSVI FIWLGL+LLAW LLF+QSS RSKK  EILN
Sbjct: 241 NCLVFLIERNFLLKRKVLYFVYGLRKSVIIFIWLGLVLLAWGLLFDQSSKRSKKGNEILN 300

Query: 301 YITRALGGSIIGATLWLVKTLLVKIVAASFQCAKFFERIKESFFHNYILQTLSRPPLMTM 360
           YITRALG S+IGA LWLVKTL+VKI+AASFQCA+FF+RI+ES FH YIL+ LS PP+M M
Sbjct: 301 YITRALGASLIGAGLWLVKTLMVKILAASFQCARFFDRIQESIFHQYILRILSGPPIMEM 360

Query: 361 TERVRRVASTGHLCYFKFLKKERDGKKEGKKGGIDVEKLRKMKQEEISAWTMSGLIDDIR 420
            E V R ASTG L  F+ LKKE DG  EGK+  IDV+KL+KMKQE+ISAWTM GLI+ IR
Sbjct: 361 AESVGRAASTGQLS-FRHLKKESDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIR 420

Query: 421 NSGLSTISDTIENFNEEEAEQKDKEIKNEWEAKAAANQIFRNVAKAGSKFIEEEDLFPFM 480
           +SGLSTIS+TIENF EEE EQKDKEI +EWEA+AAA QIFRNVAK GSK+I+E+DLF FM
Sbjct: 421 SSGLSTISNTIENFKEEENEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEDDLFRFM 480

Query: 481 RKEVVNNVLKLFEGAVENRRIKRKTLKNWLVKVYQERKSLAQSLNDTKTAIEELNRLASA 540
            KE ++NVL LFEG VE  +IKRKTLKNWLV VY ERKSLA SLNDTKTAIEELN+LASA
Sbjct: 481 SKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNKLASA 540

Query: 541 FVLFVAIIVWLLVMGFLTMKVLVFISSQLLLVAFMFGNTAKTVFEAIIFVFVMHPFDVGD 600
            VL V II WLL+MGFLT +VLVF+SSQ+LLV FMFGNTA+TVFEAIIFVFVMHPFDVGD
Sbjct: 541 IVLIVIIIEWLLLMGFLTTQVLVFVSSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVGD 600

Query: 601 RCVVDGVQMIVEEMNLLTTVFLKYDNEMIIYPNSVLATKPISNFYRSPPKMIDLVEFTVD 660
           RCVVDGVQM+VEEMN+LTT+FL+YDNE I YPNSVLATKPISN+YRS P+M D +EF+VD
Sbjct: 601 RCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRS-PEMSDSIEFSVD 660

Query: 661 FSTSIKSIEDLKAGIKSYIAKNPKIWSSNHSFLVKDIENVDKMKMALYVNHTMNFQEYTL 720
           FSTSI+SI  LKA IK+Y+   P+ W  NHS +VK+IEN++KMK+AL VNHT+NFQ Y  
Sbjct: 661 FSTSIESIGALKARIKTYLESKPQFWRPNHSVIVKEIENINKMKLALCVNHTINFQNYGD 720

Query: 721 KSNRRSELVLELKRIFENLGIKYHLLPQQVQLNY-GSSPALPPS 732
           KS+RRS+LVLELK+IFE+LGIKYHLLPQ+VQLNY GS+ ++ PS
Sbjct: 721 KSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVGSAASVIPS 762

BLAST of Tan0004158 vs. NCBI nr
Match: XP_023515637.1 (mechanosensitive ion channel protein 10-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 965.7 bits (2495), Expect = 2.3e-277
Identity = 539/762 (70.73%), Postives = 615/762 (80.71%), Query Frame = 0

Query: 1   MADKKGVEQVVVPILDSEGIVESSEE---RSVASFPDLEINDTQSFRCTIPLSVVETSPS 60
           MADKKG EQVV+ ILD E  V++  +    SVAS PD E  +T+S RC IP SVVE+SPS
Sbjct: 1   MADKKGTEQVVLRILDGEEGVDARNDLTNGSVASVPDFEPKETRSVRCAIPQSVVESSPS 60

Query: 61  HEIVRMSPLKPPKI---------------SMPKS---YPAC---ASLAEEKAQAKSTSGS 120
           HEI RMS LKPPKI               S PKS    P C   ASL EE AQAKS SGS
Sbjct: 61  HEISRMSSLKPPKIPVSAVRRPSFARSSFSKPKSRLIEPPCPDGASLTEENAQAKSASGS 120

Query: 121 LFCSSPK------ITTVTSPRDALQSAPRTPK-----GSGGEEDDNEEDYKTAEQKVKER 180
            F SSPK      I T TSP+++L+SAP TP+      +G EE+D+EE YKTAE KVKE+
Sbjct: 121 PFSSSPKRDSPANIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKEK 180

Query: 181 SRKKSKIPILIELGAFLCITGCLIASLTIDKLLTKEIWGLGLWRWCVLILVIFSGRLFSQ 240
           SRKK K  +LIE  AFLCIT CLI+SLTIDKLLTKEIWGL LW+WCVL+LVIF GRLFSQ
Sbjct: 181 SRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLELWKWCVLVLVIFCGRLFSQ 240

Query: 241 WFIKCLVFLFERNFIFQEKVLYFIYGLRKSVIFFIWLGLILLAWVLLFNQSSDRSKKDKE 300
           WFI CLVFL ERNF+ + KVLYF+YGLRKSVI  IWL L+LLAW LLF+QS  RSKK  E
Sbjct: 241 WFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVLIWLALVLLAWGLLFDQSIKRSKKVNE 300

Query: 301 ILNYITRALGGSIIGATLWLVKTLLVKIVAASFQCAKFFERIKESFFHNYILQTLSRPPL 360
           ILNY+TRAL  S+IGA LWLVKTLLVKI+AASFQC +FF+RI+ES FH YIL+TLS PPL
Sbjct: 301 ILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPL 360

Query: 361 MTMTERVRRVASTGHLCYFKFLKKERDGKKEGKKGGIDVEKLRKMKQEEISAWTMSGLID 420
           M M ERV RVAS+G L  F+ LKKERDG  EGK+  IDV+KL+KMKQE++SAWTM GLI+
Sbjct: 361 MEMAERVGRVASSGQLS-FRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLIN 420

Query: 421 DIRNSGLSTISDTIENFNEEEAEQKDKEIKNEWEAKAAANQIFRNVAKAGSKFIEEEDLF 480
            IR+SGLSTIS+TIENF EEE EQKDKEI +EWEA AAA QIFRNVAK GSK+I+EEDLF
Sbjct: 421 VIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDLF 480

Query: 481 PFMRKEVVNNVLKLFEGAVENRRIKRKTLKNWLVKVYQERKSLAQSLNDTKTAIEELNRL 540
            FM KE ++NVL LFEG VE  +IKRKTLKNWLV VY ERKSLA SLNDTKTAIEELNRL
Sbjct: 481 RFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNRL 540

Query: 541 ASAFVLFVAIIVWLLVMGFLTMKVLVFISSQLLLVAFMFGNTAKTVFEAIIFVFVMHPFD 600
           ASAFVL V +IVWLL+MGFLT +VLVFISSQLLLV FMFGNTAKT+FEAIIFVFVMHPFD
Sbjct: 541 ASAFVLIVIVIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPFD 600

Query: 601 VGDRCVVDGVQMIVEEMNLLTTVFLKYDNEMIIYPNSVLATKPISNFYRSPPKMIDLVEF 660
           VGDRCVVDGVQMIVEEMN+LTT+FL+YDNE I YPNSVLATKPISNFYRS P+M D ++F
Sbjct: 601 VGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRS-PEMSDSIDF 660

Query: 661 TVDFSTSIKSIEDLKAGIKSYIAKNPKIWSSNHSFLVKDIENVDKMKMALYVNHTMNFQE 720
           +VDFSTSI+SI  LKA IKSY+   P+ W  NHS +VK+IENV+KMK++LY+NHT+NFQ 
Sbjct: 661 SVDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQN 720

Query: 721 YTLKSNRRSELVLELKRIFENLGIKYHLLPQQVQLNYGSSPA 728
           Y  +S+RRS+LVLELK+IFE+LGIKYHLLPQ+VQLNY S+ A
Sbjct: 721 YGDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSAVA 760

BLAST of Tan0004158 vs. NCBI nr
Match: XP_022960737.1 (mechanosensitive ion channel protein 10-like [Cucurbita moschata] >XP_022960738.1 mechanosensitive ion channel protein 10-like [Cucurbita moschata])

HSP 1 Score: 964.5 bits (2492), Expect = 5.1e-277
Identity = 539/763 (70.64%), Postives = 614/763 (80.47%), Query Frame = 0

Query: 1   MADKKGVEQVVVPILDSEGIVESSE---ERSVASFPDLEINDTQSFRCTIPLSVVETSPS 60
           MADKKG+EQVV+ ILD E  V++       SVASFPD E  +T+S RC I  SVVE+SPS
Sbjct: 1   MADKKGMEQVVLRILDGEEGVDARNGLTNGSVASFPDFEPKETRSLRCAITQSVVESSPS 60

Query: 61  HEIVRMSPLKPPKI----------------SMPKS------YPACASLAEEKAQAKSTSG 120
           HEI RMS  KPPKI                S PKS       P  ASLAEE AQAKS SG
Sbjct: 61  HEISRMSSSKPPKIPVESAVRRPSFARSSFSKPKSRLIEPPRPDGASLAEENAQAKSASG 120

Query: 121 SLFCSSP------KITTVTSPRDALQSAPRTPK-----GSGGEEDDNEEDYKTAEQKVKE 180
           S F SSP      KI T TSP+++L+SAP TP+      +G EE+D+EE YKTAE KVKE
Sbjct: 121 SPFSSSPKMDSPAKIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180

Query: 181 RSRKKSKIPILIELGAFLCITGCLIASLTIDKLLTKEIWGLGLWRWCVLILVIFSGRLFS 240
           +SRKK K  ++IE  AFLCIT CLI+SLTIDKLLTKEIWGLGLW+WCVL+LVIF GRLFS
Sbjct: 181 KSRKKLKRAVVIEWIAFLCITACLISSLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFS 240

Query: 241 QWFIKCLVFLFERNFIFQEKVLYFIYGLRKSVIFFIWLGLILLAWVLLFNQSSDRSKKDK 300
           QWFI CLVFL ERNF+ + KVLYF+YGLRKSVI FIWL L+LLAW LLF+QS  RSKK  
Sbjct: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLALVLLAWGLLFDQSIKRSKKVN 300

Query: 301 EILNYITRALGGSIIGATLWLVKTLLVKIVAASFQCAKFFERIKESFFHNYILQTLSRPP 360
           EILNY+TRAL  S+IGA LWLVKTLLVKI+AASFQC +FF+RI+ES FH YIL+TLS PP
Sbjct: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360

Query: 361 LMTMTERVRRVASTGHLCYFKFLKKERDGKKEGKKGGIDVEKLRKMKQEEISAWTMSGLI 420
           LM M ERV   AS+G L  F+ LKKERDG  EGK+  IDV+KL+KMKQE++SAWTM GLI
Sbjct: 361 LMEMAERVGGAASSGQLS-FRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLI 420

Query: 421 DDIRNSGLSTISDTIENFNEEEAEQKDKEIKNEWEAKAAANQIFRNVAKAGSKFIEEEDL 480
           + IR+SGLSTIS+TIENF EEE EQKDKEI +EWEA AAA QIFRNVAK GSK+I+EEDL
Sbjct: 421 NVIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDL 480

Query: 481 FPFMRKEVVNNVLKLFEGAVENRRIKRKTLKNWLVKVYQERKSLAQSLNDTKTAIEELNR 540
           F FM KE ++NVL LFEG VE  +IKRKTLKNWLV VY ERKSLA SLNDTKTAIEELNR
Sbjct: 481 FRFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNR 540

Query: 541 LASAFVLFVAIIVWLLVMGFLTMKVLVFISSQLLLVAFMFGNTAKTVFEAIIFVFVMHPF 600
           LASAFVL V IIVWLL+MGFLT +VLVFISSQLLLV FMFGNTAKT+FEAIIFVFVMHPF
Sbjct: 541 LASAFVLIVIIIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPF 600

Query: 601 DVGDRCVVDGVQMIVEEMNLLTTVFLKYDNEMIIYPNSVLATKPISNFYRSPPKMIDLVE 660
           DVGDRCVVDGVQMIVEEMN+LTT+FL+YDNE I YPNSVLATKPISNFYRS P+M D ++
Sbjct: 601 DVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRS-PEMSDSID 660

Query: 661 FTVDFSTSIKSIEDLKAGIKSYIAKNPKIWSSNHSFLVKDIENVDKMKMALYVNHTMNFQ 720
           F+VDFSTSI+SI  LKA IKSY+   P+ W  NHS +VK+IENV+KMK++LY+NHT+NFQ
Sbjct: 661 FSVDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQ 720

Query: 721 EYTLKSNRRSELVLELKRIFENLGIKYHLLPQQVQLNYGSSPA 728
            Y  +S+RRS+LVLELK+IFE+LGIKYHLLPQ+VQLNY SS A
Sbjct: 721 NYGDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYLSSVA 761

BLAST of Tan0004158 vs. ExPASy TrEMBL
Match: A0A6J1JDR1 (Mechanosensitive ion channel protein OS=Cucurbita maxima OX=3661 GN=LOC111484882 PE=3 SV=1)

HSP 1 Score: 970.3 bits (2507), Expect = 4.5e-279
Identity = 543/763 (71.17%), Postives = 615/763 (80.60%), Query Frame = 0

Query: 1   MADKKGVEQVVVPILDSEGIVESSE---ERSVASFPDLEINDTQSFRCTIPLSVVETSPS 60
           MADKKG EQVV+ ILD E  V++       SVASFPD E  +T+S RC IP SVVE+SPS
Sbjct: 1   MADKKGTEQVVLRILDGEEGVDARNGLTNGSVASFPDFESKETRSVRCAIPQSVVESSPS 60

Query: 61  HEIVRMSPLKPPKI----------------SMPKS---YPAC---ASLAEEKAQAKSTSG 120
           HEI RMS LKPPKI                S PKS    P C   AS AEE AQAKSTSG
Sbjct: 61  HEISRMSSLKPPKIPVESAVRRPSFARSSFSKPKSRLIEPPCPDGASSAEENAQAKSTSG 120

Query: 121 SLFCSSP------KITTVTSPRDALQSAPRTPK-----GSGGEEDDNEEDYKTAEQKVKE 180
           S F SSP      KI T TSP+++L+SAP TP+      +G EE+D+EE YKTAE KVKE
Sbjct: 121 SPFSSSPKMDSPAKIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180

Query: 181 RSRKKSKIPILIELGAFLCITGCLIASLTIDKLLTKEIWGLGLWRWCVLILVIFSGRLFS 240
           +SRKK K  +LIE  AFLCIT CLI+SLTIDKLLTKEIWGLGLW+WCVL+LVIF GRLFS
Sbjct: 181 KSRKKLKRAVLIEWIAFLCITACLISSLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFS 240

Query: 241 QWFIKCLVFLFERNFIFQEKVLYFIYGLRKSVIFFIWLGLILLAWVLLFNQSSDRSKKDK 300
           QWFI CLVFL ERNF+ + KVLYF+YGLRKSVI FIWL L+LLAW LLF+QS  RSKK  
Sbjct: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLALVLLAWGLLFDQSIKRSKKVN 300

Query: 301 EILNYITRALGGSIIGATLWLVKTLLVKIVAASFQCAKFFERIKESFFHNYILQTLSRPP 360
           EILNY+TRAL  S+IGA LWLVKTLLVKI+AASFQC +FF+RI+ES FH YIL+ LS PP
Sbjct: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPP 360

Query: 361 LMTMTERVRRVASTGHLCYFKFLKKERDGKKEGKKGGIDVEKLRKMKQEEISAWTMSGLI 420
           LM M ERV RVAS G L  F+ LKKERDG  EGK+  IDV+KL+KMKQ ++SAWTM GLI
Sbjct: 361 LMEMAERVGRVASAGQLS-FRHLKKERDGGNEGKEEVIDVDKLKKMKQAKVSAWTMRGLI 420

Query: 421 DDIRNSGLSTISDTIENFNEEEAEQKDKEIKNEWEAKAAANQIFRNVAKAGSKFIEEEDL 480
           + IR+SGLSTIS+TIENF EEE EQKDKEI +EWEA AAA QIFRNVAK GSK+I+EEDL
Sbjct: 421 NVIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDL 480

Query: 481 FPFMRKEVVNNVLKLFEGAVENRRIKRKTLKNWLVKVYQERKSLAQSLNDTKTAIEELNR 540
           F FM KE ++NVL LFEG VE  +IKRKTLKNWLV VY ERKSLA SLNDTKTAIEELNR
Sbjct: 481 FRFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNR 540

Query: 541 LASAFVLFVAIIVWLLVMGFLTMKVLVFISSQLLLVAFMFGNTAKTVFEAIIFVFVMHPF 600
           LASAFVL V IIVWLL+MGFLT +VLVFISSQLLLV FMFGNTAKT+FEAIIFVFVMHPF
Sbjct: 541 LASAFVLIVIIIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPF 600

Query: 601 DVGDRCVVDGVQMIVEEMNLLTTVFLKYDNEMIIYPNSVLATKPISNFYRSPPKMIDLVE 660
           DVGDRCVVDGVQMIVEEMN+LTT+FL+YDNE I YPNSVLATKPISNFYRS P+M D ++
Sbjct: 601 DVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRS-PEMSDSID 660

Query: 661 FTVDFSTSIKSIEDLKAGIKSYIAKNPKIWSSNHSFLVKDIENVDKMKMALYVNHTMNFQ 720
           F+VDFSTSI+SI  LKA IKSY+   P+ W  NHS +VK+IENV+KMK++LY+NHT+NFQ
Sbjct: 661 FSVDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQ 720

Query: 721 EYTLKSNRRSELVLELKRIFENLGIKYHLLPQQVQLNYGSSPA 728
            Y  +S+RRS+LVLELK+IFE+LGIKYHLLPQ+VQLNY SS A
Sbjct: 721 NYGDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYVSSIA 761

BLAST of Tan0004158 vs. ExPASy TrEMBL
Match: A0A6J1H896 (Mechanosensitive ion channel protein OS=Cucurbita moschata OX=3662 GN=LOC111461449 PE=3 SV=1)

HSP 1 Score: 964.5 bits (2492), Expect = 2.5e-277
Identity = 539/763 (70.64%), Postives = 614/763 (80.47%), Query Frame = 0

Query: 1   MADKKGVEQVVVPILDSEGIVESSE---ERSVASFPDLEINDTQSFRCTIPLSVVETSPS 60
           MADKKG+EQVV+ ILD E  V++       SVASFPD E  +T+S RC I  SVVE+SPS
Sbjct: 1   MADKKGMEQVVLRILDGEEGVDARNGLTNGSVASFPDFEPKETRSLRCAITQSVVESSPS 60

Query: 61  HEIVRMSPLKPPKI----------------SMPKS------YPACASLAEEKAQAKSTSG 120
           HEI RMS  KPPKI                S PKS       P  ASLAEE AQAKS SG
Sbjct: 61  HEISRMSSSKPPKIPVESAVRRPSFARSSFSKPKSRLIEPPRPDGASLAEENAQAKSASG 120

Query: 121 SLFCSSP------KITTVTSPRDALQSAPRTPK-----GSGGEEDDNEEDYKTAEQKVKE 180
           S F SSP      KI T TSP+++L+SAP TP+      +G EE+D+EE YKTAE KVKE
Sbjct: 121 SPFSSSPKMDSPAKIATATSPKESLKSAPITPRTPLVGSTGSEEEDDEEVYKTAELKVKE 180

Query: 181 RSRKKSKIPILIELGAFLCITGCLIASLTIDKLLTKEIWGLGLWRWCVLILVIFSGRLFS 240
           +SRKK K  ++IE  AFLCIT CLI+SLTIDKLLTKEIWGLGLW+WCVL+LVIF GRLFS
Sbjct: 181 KSRKKLKRAVVIEWIAFLCITACLISSLTIDKLLTKEIWGLGLWKWCVLVLVIFCGRLFS 240

Query: 241 QWFIKCLVFLFERNFIFQEKVLYFIYGLRKSVIFFIWLGLILLAWVLLFNQSSDRSKKDK 300
           QWFI CLVFL ERNF+ + KVLYF+YGLRKSVI FIWL L+LLAW LLF+QS  RSKK  
Sbjct: 241 QWFINCLVFLIERNFLLKRKVLYFVYGLRKSVIVFIWLALVLLAWGLLFDQSIKRSKKVN 300

Query: 301 EILNYITRALGGSIIGATLWLVKTLLVKIVAASFQCAKFFERIKESFFHNYILQTLSRPP 360
           EILNY+TRAL  S+IGA LWLVKTLLVKI+AASFQC +FF+RI+ES FH YIL+TLS PP
Sbjct: 301 EILNYVTRALAASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPP 360

Query: 361 LMTMTERVRRVASTGHLCYFKFLKKERDGKKEGKKGGIDVEKLRKMKQEEISAWTMSGLI 420
           LM M ERV   AS+G L  F+ LKKERDG  EGK+  IDV+KL+KMKQE++SAWTM GLI
Sbjct: 361 LMEMAERVGGAASSGQLS-FRHLKKERDGGNEGKEEVIDVDKLKKMKQEKVSAWTMRGLI 420

Query: 421 DDIRNSGLSTISDTIENFNEEEAEQKDKEIKNEWEAKAAANQIFRNVAKAGSKFIEEEDL 480
           + IR+SGLSTIS+TIENF EEE EQKDKEI +EWEA AAA QIFRNVAK GSK+I+EEDL
Sbjct: 421 NVIRSSGLSTISNTIENFKEEEGEQKDKEINSEWEASAAAYQIFRNVAKPGSKYIDEEDL 480

Query: 481 FPFMRKEVVNNVLKLFEGAVENRRIKRKTLKNWLVKVYQERKSLAQSLNDTKTAIEELNR 540
           F FM KE ++NVL LFEG VE  +IKRKTLKNWLV VY ERKSLA SLNDTKTAIEELNR
Sbjct: 481 FRFMNKEEIDNVLPLFEGGVETGKIKRKTLKNWLVNVYIERKSLAHSLNDTKTAIEELNR 540

Query: 541 LASAFVLFVAIIVWLLVMGFLTMKVLVFISSQLLLVAFMFGNTAKTVFEAIIFVFVMHPF 600
           LASAFVL V IIVWLL+MGFLT +VLVFISSQLLLV FMFGNTAKT+FEAIIFVFVMHPF
Sbjct: 541 LASAFVLIVIIIVWLLLMGFLTTQVLVFISSQLLLVVFMFGNTAKTIFEAIIFVFVMHPF 600

Query: 601 DVGDRCVVDGVQMIVEEMNLLTTVFLKYDNEMIIYPNSVLATKPISNFYRSPPKMIDLVE 660
           DVGDRCVVDGVQMIVEEMN+LTT+FL+YDNE I YPNSVLATKPISNFYRS P+M D ++
Sbjct: 601 DVGDRCVVDGVQMIVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNFYRS-PEMSDSID 660

Query: 661 FTVDFSTSIKSIEDLKAGIKSYIAKNPKIWSSNHSFLVKDIENVDKMKMALYVNHTMNFQ 720
           F+VDFSTSI+SI  LKA IKSY+   P+ W  NHS +VK+IENV+KMK++LY+NHT+NFQ
Sbjct: 661 FSVDFSTSIESIGALKARIKSYLESKPQFWRPNHSVIVKEIENVNKMKLSLYINHTINFQ 720

Query: 721 EYTLKSNRRSELVLELKRIFENLGIKYHLLPQQVQLNYGSSPA 728
            Y  +S+RRS+LVLELK+IFE+LGIKYHLLPQ+VQLNY SS A
Sbjct: 721 NYGDRSSRRSDLVLELKKIFEDLGIKYHLLPQEVQLNYLSSVA 761

BLAST of Tan0004158 vs. ExPASy TrEMBL
Match: A0A6J1D1P7 (Mechanosensitive ion channel protein OS=Momordica charantia OX=3673 GN=LOC111016536 PE=3 SV=1)

HSP 1 Score: 962.6 bits (2487), Expect = 9.3e-277
Identity = 540/764 (70.68%), Postives = 617/764 (80.76%), Query Frame = 0

Query: 1   MADKKGVEQVVVPILDSEGIVESSEE---RSVASFPDLEINDTQSFRCTIPLSVVETSPS 60
           MADK+G EQVV+ ILDSEG V+ +++   RSVAS PD E+ +T+S RCTIP S V +SPS
Sbjct: 1   MADKEGTEQVVLRILDSEGAVDGTKDHTKRSVASHPDFELKETRSIRCTIPQSGVGSSPS 60

Query: 61  HEIVRMSPLKPPKI----------------SMPKS------YPACASLAEEKAQAKSTSG 120
            EI RMSPLKPPKI                S PKS       P  A+LAE+KAQAKSTSG
Sbjct: 61  PEIARMSPLKPPKIPVESTVRRPSFARSSFSKPKSRLVEPPCPGGANLAEDKAQAKSTSG 120

Query: 121 SLFCSSP-KITTVTSPRDALQSAPRTPK----GS-GGEEDDNEEDYKTAEQKVKERSRKK 180
           S + +SP KITT TSPRDAL+SAP TPK    GS G EE+D+EE YKTAE KVKERS +K
Sbjct: 121 SPYNNSPAKITTATSPRDALKSAPITPKTPLIGSIGSEEEDDEEVYKTAELKVKERSGRK 180

Query: 181 SKIPILIELGAFLCITGCLIASLTIDKLLTKEIWGLGLWRWCVLILVIFSGRLFSQWFIK 240
            KI ILIE  AFLCITGC IASLTI KL T EIWGLGLW+WCVL+LVIF GRLFSQWFI 
Sbjct: 181 LKITILIEWVAFLCITGCFIASLTIHKLQTTEIWGLGLWQWCVLVLVIFCGRLFSQWFIN 240

Query: 241 CLVFLFERNFIFQEKVLYFIYGLRKSVIFFIWLGLILLAWVLLFNQSSDRSKKDKEILNY 300
           CLVFL ERNF+ + KVLYF+YGLRKSVI FIWL L+LLAW LLF+ S  RSKK  +ILNY
Sbjct: 241 CLVFLIERNFLLRRKVLYFVYGLRKSVIIFIWLALVLLAWGLLFDHSRKRSKKANDILNY 300

Query: 301 ITRALGGSIIGATLWLVKTLLVKIVAASFQCAKFFERIKESFFHNYILQTLSRPPLMTMT 360
           +TRAL  S+ GA LWL+KTLLVKI+AASFQC +FF+RI+ES FH YIL+TLS PPL+ M 
Sbjct: 301 VTRALAASLAGAGLWLLKTLLVKILAASFQCTRFFDRIQESIFHQYILRTLSGPPLIEMA 360

Query: 361 ERVRRVASTGHLCYFKFLKKERDGKKEGKKGGIDVEKLRKMKQEEISAWTMSGLIDDIRN 420
           ERV R ASTG L  F+ LKK+ DG  EGK+  IDV+KL+KMKQE+ISAWTM GLI+ IR+
Sbjct: 361 ERVGRTASTGQLS-FRHLKKQSDGGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVIRS 420

Query: 421 SGLSTISDTIENFNEEEAEQKDKEIKNEWEAKAAANQIFRNVAKAGSKFIEEEDLFPFMR 480
           SGLSTIS+TIENFNEEE+EQKDKEI +EWEA+AAA QIFRNVAK GSK+I+EEDLF FM 
Sbjct: 421 SGLSTISNTIENFNEEESEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRFMS 480

Query: 481 KEVVNNVLKLFEGAVENRRIKRKTLKNWLVKVYQERKSLAQSLNDTKTAIEELNRLASAF 540
           KE V NVL LFEGAVE  +IKRKTLKNWLV VY ERKSLA SLNDTKTAIEELN+LASA 
Sbjct: 481 KEEVENVLPLFEGAVETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLASAV 540

Query: 541 VLFVAIIVWLLVMGFLTMKVLVFISSQLLLVAFMFGNTAKTVFEAIIFVFVMHPFDVGDR 600
           VL V IIVWLL+MG LT ++LVFISSQLLLV FMFGNTA+TVFEAIIFVFVMHPFDVGDR
Sbjct: 541 VLIVIIIVWLLLMGLLTTQILVFISSQLLLVVFMFGNTARTVFEAIIFVFVMHPFDVGDR 600

Query: 601 CVVDGVQMIVEEMNLLTTVFLKYDNEMIIYPNSVLATKPISNFYRSPPKMIDLVEFTVDF 660
           CVVDGVQM+VEEMN+LTT+FL++DNE I YPNSVLATKPISNFYRS P+M D +EF+VDF
Sbjct: 601 CVVDGVQMVVEEMNILTTIFLRFDNEKIFYPNSVLATKPISNFYRS-PEMSDSIEFSVDF 660

Query: 661 STSIKSIEDLKAGIKSYIAKNPKIWSSNHSFLVKDIENVDKMKMALYVNHTMNFQEYTLK 720
           STSI+SI  LKA IKSY+   P+ W   HS +VK+IENV+KMKM LYVNHT+NFQ Y  K
Sbjct: 661 STSIESIGALKARIKSYLESKPQFWRPGHSVVVKEIENVNKMKMGLYVNHTINFQNYGDK 720

Query: 721 SNRRSELVLELKRIFENLGIKYHLLPQQVQLNY--GSSPALPPS 732
           S+RRS+LVLELK+I E LGIKYHLLPQ+V LN    ++P +PPS
Sbjct: 721 SSRRSDLVLELKKILEELGIKYHLLPQEVYLNSVGSAAPMVPPS 762

BLAST of Tan0004158 vs. ExPASy TrEMBL
Match: A0A1S3BR08 (Mechanosensitive ion channel protein OS=Cucumis melo OX=3656 GN=LOC103492355 PE=3 SV=1)

HSP 1 Score: 944.5 bits (2440), Expect = 2.6e-271
Identity = 531/769 (69.05%), Postives = 617/769 (80.23%), Query Frame = 0

Query: 1   MADKKGVEQVVVPILD-SEGIVESSEE---RSVASFPDLEINDTQSFRCTIPLSVVETSP 60
           MADKKG+EQ+V+ IL+  EG+  SS++    SV SFPD ++ +T+SFRCTIP S+V +SP
Sbjct: 1   MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPRSLVGSSP 60

Query: 61  SHEIVRMSPLKPPKI----------------SMPKS---YPAC---ASLAEEKAQAKST- 120
           SHEI RM+PLKPPKI                S PKS    P C    SLAEEKA AKS+ 
Sbjct: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIEPPCPDGESLAEEKALAKSSL 120

Query: 121 SGSLFCSSP-KITTVTSPRDALQSAPRTPK-----GSGGEEDDNEEDYKTAEQKVKERSR 180
            GS    SP KIT VTSP++AL++ P TPK      +G EE+D+EE YKTAE KVKE+S 
Sbjct: 121 YGSPKVDSPAKITAVTSPKEALKATPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSE 180

Query: 181 KKSKIPILIELGAFLCITGCLIASLTIDKLLTKEIWGLGLWRWCVLILVIFSGRLFSQWF 240
           K+ K  +++E  AFLC+TGCLIASLTID L+TKEIWGLGLW+WCVL+LVIF GRLFSQWF
Sbjct: 181 KRLKKTVIVEWVAFLCLTGCLIASLTIDTLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240

Query: 241 IKCLVFLFERNFIFQEKVLYFIYGLRKSVIFFIWLGLILLAWVLLFNQSSDRSKKDKEIL 300
           I CLVFL ERNF+ + KVLYF+YGL+KSVI FIWL L+LLAW LLF+QSS RSKK  EIL
Sbjct: 241 INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL 300

Query: 301 NYITRALGGSIIGATLWLVKTLLVKIVAASFQCAKFFERIKESFFHNYILQTLSRPPLMT 360
           NY+TRALG S+IGA LWLVKTLLVKI+AASFQC +FF+RI+ES FH YIL+ LS PPLM 
Sbjct: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360

Query: 361 MTERVRRVASTGHLCYFKFLKKERDGKKEGKKGGIDVEKLRKMKQEEISAWTMSGLIDDI 420
           M  RV R ASTG L  FK LK+E D   EGK+  IDV+KL+KMKQE+ISAWTM GLI+ I
Sbjct: 361 MAGRVGRAASTGQLS-FKHLKQESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVI 420

Query: 421 RNSGLSTISDTIENFNEEEAEQKDKEIKNEWEAKAAANQIFRNVAKAGSKFIEEEDLFPF 480
           R SGLSTIS+TIENF EEE EQKDKEI +EWEA+AAA QIFRNVAK GSK+I+EEDLF F
Sbjct: 421 RGSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRF 480

Query: 481 MRKEVVNNVLKLFEGAVENRRIKRKTLKNWLVKVYQERKSLAQSLNDTKTAIEELNRLAS 540
           M KE ++NVL LFEG  E  +IKRKTLKNWLV VY ERKSLA SLNDTKTAIEELN+LAS
Sbjct: 481 MSKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLAS 540

Query: 541 AFVLFVAIIVWLLVMGFLTMKVLVFISSQLLLVAFMFGNTAKTVFEAIIFVFVMHPFDVG 600
           A VL V II WLL+MGFLT +VLVFISSQ+LLV FMFGNTA+TVFEAIIFVFVMHPFDVG
Sbjct: 541 AVVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVG 600

Query: 601 DRCVVDGVQMIVEEMNLLTTVFLKYDNEMIIYPNSVLATKPISNFYRSPPKMIDLVEFTV 660
           DRCVVDGVQM+VEEMN+LTT+FL+YDNE I YPNSVLATKPISN+YRS P+M D ++F+V
Sbjct: 601 DRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRS-PEMSDSIDFSV 660

Query: 661 DFSTSIKSIEDLKAGIKSYIAKNPKIWSSNHSFLVKDIENVDKMKMALYVNHTMNFQEYT 720
           DFSTSI+SI  LKA IK+Y+   P+ W  N+S +VK+IENV+KMK+AL VNHT+NFQ Y 
Sbjct: 661 DFSTSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYG 720

Query: 721 LKSNRRSELVLELKRIFENLGIKYHLLPQQVQLNYGSSPA----LPPSR 733
            KSNRRS+LVLELK+IFE+LGIKYHLLPQ VQLNY SS A    LPPS+
Sbjct: 721 DKSNRRSDLVLELKKIFEDLGIKYHLLPQAVQLNYASSAAGGVPLPPSQ 767

BLAST of Tan0004158 vs. ExPASy TrEMBL
Match: A0A5D3CFQ7 (Mechanosensitive ion channel protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001770 PE=3 SV=1)

HSP 1 Score: 944.5 bits (2440), Expect = 2.6e-271
Identity = 531/769 (69.05%), Postives = 617/769 (80.23%), Query Frame = 0

Query: 1   MADKKGVEQVVVPILD-SEGIVESSEE---RSVASFPDLEINDTQSFRCTIPLSVVETSP 60
           MADKKG+EQ+V+ IL+  EG+  SS++    SV SFPD ++ +T+SFRCTIP S+V +SP
Sbjct: 1   MADKKGMEQLVLRILEGEEGVHGSSKDLNKPSVGSFPDFDLKETRSFRCTIPRSLVGSSP 60

Query: 61  SHEIVRMSPLKPPKI----------------SMPKS---YPAC---ASLAEEKAQAKST- 120
           SHEI RM+PLKPPKI                S PKS    P C    SLAEEKA AKS+ 
Sbjct: 61  SHEISRMTPLKPPKIPGETVTRRASFACSSFSKPKSRLIEPPCPDGESLAEEKALAKSSL 120

Query: 121 SGSLFCSSP-KITTVTSPRDALQSAPRTPK-----GSGGEEDDNEEDYKTAEQKVKERSR 180
            GS    SP KIT VTSP++AL++ P TPK      +G EE+D+EE YKTAE KVKE+S 
Sbjct: 121 YGSPKVDSPAKITAVTSPKEALKATPITPKTPLIGTTGNEEEDDEEVYKTAELKVKEKSE 180

Query: 181 KKSKIPILIELGAFLCITGCLIASLTIDKLLTKEIWGLGLWRWCVLILVIFSGRLFSQWF 240
           K+ K  +++E  AFLC+TGCLIASLTID L+TKEIWGLGLW+WCVL+LVIF GRLFSQWF
Sbjct: 181 KRLKKTVIVEWVAFLCLTGCLIASLTIDTLVTKEIWGLGLWKWCVLVLVIFCGRLFSQWF 240

Query: 241 IKCLVFLFERNFIFQEKVLYFIYGLRKSVIFFIWLGLILLAWVLLFNQSSDRSKKDKEIL 300
           I CLVFL ERNF+ + KVLYF+YGL+KSVI FIWL L+LLAW LLF+QSS RSKK  EIL
Sbjct: 241 INCLVFLIERNFLLKRKVLYFVYGLKKSVIIFIWLALVLLAWGLLFDQSSKRSKKGNEIL 300

Query: 301 NYITRALGGSIIGATLWLVKTLLVKIVAASFQCAKFFERIKESFFHNYILQTLSRPPLMT 360
           NY+TRALG S+IGA LWLVKTLLVKI+AASFQC +FF+RI+ES FH YIL+ LS PPLM 
Sbjct: 301 NYVTRALGASLIGAGLWLVKTLLVKILAASFQCTRFFDRIQESIFHQYILRILSGPPLME 360

Query: 361 MTERVRRVASTGHLCYFKFLKKERDGKKEGKKGGIDVEKLRKMKQEEISAWTMSGLIDDI 420
           M  RV R ASTG L  FK LK+E D   EGK+  IDV+KL+KMKQE+ISAWTM GLI+ I
Sbjct: 361 MAGRVGRAASTGQLS-FKHLKQESDDGNEGKEEVIDVDKLKKMKQEKISAWTMRGLINVI 420

Query: 421 RNSGLSTISDTIENFNEEEAEQKDKEIKNEWEAKAAANQIFRNVAKAGSKFIEEEDLFPF 480
           R SGLSTIS+TIENF EEE EQKDKEI +EWEA+AAA QIFRNVAK GSK+I+EEDLF F
Sbjct: 421 RGSGLSTISNTIENFKEEEVEQKDKEINSEWEARAAAYQIFRNVAKPGSKYIDEEDLFRF 480

Query: 481 MRKEVVNNVLKLFEGAVENRRIKRKTLKNWLVKVYQERKSLAQSLNDTKTAIEELNRLAS 540
           M KE ++NVL LFEG  E  +IKRKTLKNWLV VY ERKSLA SLNDTKTAIEELN+LAS
Sbjct: 481 MSKEEIDNVLPLFEGGAETGKIKRKTLKNWLVNVYVERKSLAHSLNDTKTAIEELNKLAS 540

Query: 541 AFVLFVAIIVWLLVMGFLTMKVLVFISSQLLLVAFMFGNTAKTVFEAIIFVFVMHPFDVG 600
           A VL V II WLL+MGFLT +VLVFISSQ+LLV FMFGNTA+TVFEAIIFVFVMHPFDVG
Sbjct: 541 AVVLIVIIIEWLLLMGFLTTQVLVFISSQILLVVFMFGNTARTVFEAIIFVFVMHPFDVG 600

Query: 601 DRCVVDGVQMIVEEMNLLTTVFLKYDNEMIIYPNSVLATKPISNFYRSPPKMIDLVEFTV 660
           DRCVVDGVQM+VEEMN+LTT+FL+YDNE I YPNSVLATKPISN+YRS P+M D ++F+V
Sbjct: 601 DRCVVDGVQMVVEEMNILTTIFLRYDNEKIFYPNSVLATKPISNYYRS-PEMSDSIDFSV 660

Query: 661 DFSTSIKSIEDLKAGIKSYIAKNPKIWSSNHSFLVKDIENVDKMKMALYVNHTMNFQEYT 720
           DFSTSI+SI  LKA IK+Y+   P+ W  N+S +VK+IENV+KMK+AL VNHT+NFQ Y 
Sbjct: 661 DFSTSIESIGALKARIKTYLESKPQFWRPNYSVVVKEIENVNKMKLALCVNHTINFQNYG 720

Query: 721 LKSNRRSELVLELKRIFENLGIKYHLLPQQVQLNYGSSPA----LPPSR 733
            KSNRRS+LVLELK+IFE+LGIKYHLLPQ VQLNY SS A    LPPS+
Sbjct: 721 DKSNRRSDLVLELKKIFEDLGIKYHLLPQAVQLNYASSAAGGVPLPPSQ 767

BLAST of Tan0004158 vs. TAIR 10
Match: AT5G12080.1 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 570.1 bits (1468), Expect = 2.6e-162
Identity = 345/694 (49.71%), Postives = 459/694 (66.14%), Query Frame = 0

Query: 55  SPSHEIVRM--SPLKPPK-------------------ISMPKSY---PAC---ASLAEEK 114
           SPS EI ++  SP KPP+                    S PKS    P+C    S+ EE+
Sbjct: 46  SPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEEE 105

Query: 115 AQAKSTSGSLFCSSPKITTVTSPRDALQSAPRTPKGSGGEEDDNEEDYKTAEQKVKERSR 174
            + +  +G  F  S       S R     AP TP     E+D++EE YK  + K+    R
Sbjct: 106 VREQLGAG--FSFSRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYK--KVKLNREMR 165

Query: 175 KKSKIPILIELGAFLCITGCLIASLTIDKLLTKEIWGLGLWRWCVLILVIFSGRLFSQWF 234
            K     LIE   F+ I   L+ASLTI+ L     WGL +W+WCVL++VIFSG L + WF
Sbjct: 166 SKISTLALIESAFFVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWF 225

Query: 235 IKCLVFLFERNFIFQEKVLYFIYGLRKSVIFFIWLGLILLAWVLLFNQSSDRSKKDKEIL 294
           ++ +VFL E NF+ + KVLYF++GL+KSV  FIWL LIL+AW+LLFN    RS    ++L
Sbjct: 226 MRLIVFLIETNFLLRRKVLYFVHGLKKSVQVFIWLCLILVAWILLFNHDVKRSPAATKVL 285

Query: 295 NYITRALGGSIIGATLWLVKTLLVKIVAASFQCAKFFERIKESFFHNYILQTLSRPPLMT 354
             ITR L   + GA  WLVKTLL+KI+AA+F    FF+RI++S FH Y+LQTLS  PLM 
Sbjct: 286 KCITRTLISILTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLME 345

Query: 355 MTERVRRVASTGHLCYFKFLKKERDGKKEGKKGGIDVEKLRKMKQEEISAWTMSGLIDDI 414
             ERV R  STGHL +   +KK   G  + KK  ID+ K+ KMK+E++SAWTM  L++ +
Sbjct: 346 EAERVGREPSTGHLSFATVVKK---GTVKEKK-VIDMGKVHKMKREKVSAWTMRVLMEAV 405

Query: 415 RNSGLSTISDTI-ENFNEEEAEQKDKEIKNEWEAKAAANQIFRNVAKAGSKFIEEEDLFP 474
           R SGLSTISDT+ E    E  EQ D+EI +E EA AAA  +FRNVA+    +IEEEDL  
Sbjct: 406 RTSGLSTISDTLDETAYGEGKEQADREITSEMEALAAAYHVFRNVAQPFFNYIEEEDLLR 465

Query: 475 FMRKEVVNNVLKLFEGAVENRRIKRKTLKNWLVKVYQERKSLAQSLNDTKTAIEELNRLA 534
           FM KE V+ V  LF+GA E  RI RK    W+VKVY  R++LA SLNDTKTA+++LN+L 
Sbjct: 466 FMIKEEVDLVFPLFDGAAETGRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLV 525

Query: 535 SAFVLFVAIIVWLLVMGFLTMKVLVFISSQLLLVAFMFGNTAKTVFEAIIFVFVMHPFDV 594
           +A ++ V +++WLL++   T KVL+F S+QL+ +AF+ G+T K +FE+I+FVFVMHP+DV
Sbjct: 526 TAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDV 585

Query: 595 GDRCVVDGVQMIVEEMNLLTTVFLKYDNEMIIYPNSVLATKPISNFYRSPPKMIDLVEFT 654
           GDRCVVDGV M+VEEMNLLTTVFLK +NE + YPN+VLATKPISN++RS P M + VEF+
Sbjct: 586 GDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRS-PNMGETVEFS 645

Query: 655 VDFSTSIKSIEDLKAGIKSYIAKNPKIWSSNHSFLVKDIENVDKMKMALYVNHTMNFQEY 714
           + FST +  I  LK  I  Y+ +NP+ W+  HS +VK+IEN++K+KMALY +HT+ FQE 
Sbjct: 646 ISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQEN 705

Query: 715 TLKSNRRSELVLELKRIFENLGIKYHLLPQQVQL 721
             ++ RR+EL L +KR+ E+L I Y LLPQ + L
Sbjct: 706 RERNLRRTELSLAIKRMLEDLHIDYTLLPQDINL 730

BLAST of Tan0004158 vs. TAIR 10
Match: AT5G12080.2 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 570.1 bits (1468), Expect = 2.6e-162
Identity = 345/694 (49.71%), Postives = 459/694 (66.14%), Query Frame = 0

Query: 55  SPSHEIVRM--SPLKPPK-------------------ISMPKSY---PAC---ASLAEEK 114
           SPS EI ++  SP KPP+                    S PKS    P+C    S+ EE+
Sbjct: 46  SPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEEE 105

Query: 115 AQAKSTSGSLFCSSPKITTVTSPRDALQSAPRTPKGSGGEEDDNEEDYKTAEQKVKERSR 174
            + +  +G  F  S       S R     AP TP     E+D++EE YK  + K+    R
Sbjct: 106 VREQLGAG--FSFSRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYK--KVKLNREMR 165

Query: 175 KKSKIPILIELGAFLCITGCLIASLTIDKLLTKEIWGLGLWRWCVLILVIFSGRLFSQWF 234
            K     LIE   F+ I   L+ASLTI+ L     WGL +W+WCVL++VIFSG L + WF
Sbjct: 166 SKISTLALIESAFFVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWF 225

Query: 235 IKCLVFLFERNFIFQEKVLYFIYGLRKSVIFFIWLGLILLAWVLLFNQSSDRSKKDKEIL 294
           ++ +VFL E NF+ + KVLYF++GL+KSV  FIWL LIL+AW+LLFN    RS    ++L
Sbjct: 226 MRLIVFLIETNFLLRRKVLYFVHGLKKSVQVFIWLCLILVAWILLFNHDVKRSPAATKVL 285

Query: 295 NYITRALGGSIIGATLWLVKTLLVKIVAASFQCAKFFERIKESFFHNYILQTLSRPPLMT 354
             ITR L   + GA  WLVKTLL+KI+AA+F    FF+RI++S FH Y+LQTLS  PLM 
Sbjct: 286 KCITRTLISILTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLME 345

Query: 355 MTERVRRVASTGHLCYFKFLKKERDGKKEGKKGGIDVEKLRKMKQEEISAWTMSGLIDDI 414
             ERV R  STGHL +   +KK   G  + KK  ID+ K+ KMK+E++SAWTM  L++ +
Sbjct: 346 EAERVGREPSTGHLSFATVVKK---GTVKEKK-VIDMGKVHKMKREKVSAWTMRVLMEAV 405

Query: 415 RNSGLSTISDTI-ENFNEEEAEQKDKEIKNEWEAKAAANQIFRNVAKAGSKFIEEEDLFP 474
           R SGLSTISDT+ E    E  EQ D+EI +E EA AAA  +FRNVA+    +IEEEDL  
Sbjct: 406 RTSGLSTISDTLDETAYGEGKEQADREITSEMEALAAAYHVFRNVAQPFFNYIEEEDLLR 465

Query: 475 FMRKEVVNNVLKLFEGAVENRRIKRKTLKNWLVKVYQERKSLAQSLNDTKTAIEELNRLA 534
           FM KE V+ V  LF+GA E  RI RK    W+VKVY  R++LA SLNDTKTA+++LN+L 
Sbjct: 466 FMIKEEVDLVFPLFDGAAETGRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLV 525

Query: 535 SAFVLFVAIIVWLLVMGFLTMKVLVFISSQLLLVAFMFGNTAKTVFEAIIFVFVMHPFDV 594
           +A ++ V +++WLL++   T KVL+F S+QL+ +AF+ G+T K +FE+I+FVFVMHP+DV
Sbjct: 526 TAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDV 585

Query: 595 GDRCVVDGVQMIVEEMNLLTTVFLKYDNEMIIYPNSVLATKPISNFYRSPPKMIDLVEFT 654
           GDRCVVDGV M+VEEMNLLTTVFLK +NE + YPN+VLATKPISN++RS P M + VEF+
Sbjct: 586 GDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRS-PNMGETVEFS 645

Query: 655 VDFSTSIKSIEDLKAGIKSYIAKNPKIWSSNHSFLVKDIENVDKMKMALYVNHTMNFQEY 714
           + FST +  I  LK  I  Y+ +NP+ W+  HS +VK+IEN++K+KMALY +HT+ FQE 
Sbjct: 646 ISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQEN 705

Query: 715 TLKSNRRSELVLELKRIFENLGIKYHLLPQQVQL 721
             ++ RR+EL L +KR+ E+L I Y LLPQ + L
Sbjct: 706 RERNLRRTELSLAIKRMLEDLHIDYTLLPQDINL 730

BLAST of Tan0004158 vs. TAIR 10
Match: AT5G12080.3 (mechanosensitive channel of small conductance-like 10 )

HSP 1 Score: 570.1 bits (1468), Expect = 2.6e-162
Identity = 345/694 (49.71%), Postives = 459/694 (66.14%), Query Frame = 0

Query: 55  SPSHEIVRM--SPLKPPK-------------------ISMPKSY---PAC---ASLAEEK 114
           SPS EI ++  SP KPP+                    S PKS    P+C    S+ EE+
Sbjct: 46  SPSPEISKLVGSPNKPPRAPNQNNVGLTQRKSFARSVYSKPKSRFVDPSCPVDTSILEEE 105

Query: 115 AQAKSTSGSLFCSSPKITTVTSPRDALQSAPRTPKGSGGEEDDNEEDYKTAEQKVKERSR 174
            + +  +G  F  S       S R     AP TP     E+D++EE YK  + K+    R
Sbjct: 106 VREQLGAG--FSFSRASPNNKSNRSVGSPAPVTPSKVVVEKDEDEEIYK--KVKLNREMR 165

Query: 175 KKSKIPILIELGAFLCITGCLIASLTIDKLLTKEIWGLGLWRWCVLILVIFSGRLFSQWF 234
            K     LIE   F+ I   L+ASLTI+ L     WGL +W+WCVL++VIFSG L + WF
Sbjct: 166 SKISTLALIESAFFVVILSALVASLTINVLKHHTFWGLEVWKWCVLVMVIFSGMLVTNWF 225

Query: 235 IKCLVFLFERNFIFQEKVLYFIYGLRKSVIFFIWLGLILLAWVLLFNQSSDRSKKDKEIL 294
           ++ +VFL E NF+ + KVLYF++GL+KSV  FIWL LIL+AW+LLFN    RS    ++L
Sbjct: 226 MRLIVFLIETNFLLRRKVLYFVHGLKKSVQVFIWLCLILVAWILLFNHDVKRSPAATKVL 285

Query: 295 NYITRALGGSIIGATLWLVKTLLVKIVAASFQCAKFFERIKESFFHNYILQTLSRPPLMT 354
             ITR L   + GA  WLVKTLL+KI+AA+F    FF+RI++S FH Y+LQTLS  PLM 
Sbjct: 286 KCITRTLISILTGAFFWLVKTLLLKILAANFNVNNFFDRIQDSVFHQYVLQTLSGLPLME 345

Query: 355 MTERVRRVASTGHLCYFKFLKKERDGKKEGKKGGIDVEKLRKMKQEEISAWTMSGLIDDI 414
             ERV R  STGHL +   +KK   G  + KK  ID+ K+ KMK+E++SAWTM  L++ +
Sbjct: 346 EAERVGREPSTGHLSFATVVKK---GTVKEKK-VIDMGKVHKMKREKVSAWTMRVLMEAV 405

Query: 415 RNSGLSTISDTI-ENFNEEEAEQKDKEIKNEWEAKAAANQIFRNVAKAGSKFIEEEDLFP 474
           R SGLSTISDT+ E    E  EQ D+EI +E EA AAA  +FRNVA+    +IEEEDL  
Sbjct: 406 RTSGLSTISDTLDETAYGEGKEQADREITSEMEALAAAYHVFRNVAQPFFNYIEEEDLLR 465

Query: 475 FMRKEVVNNVLKLFEGAVENRRIKRKTLKNWLVKVYQERKSLAQSLNDTKTAIEELNRLA 534
           FM KE V+ V  LF+GA E  RI RK    W+VKVY  R++LA SLNDTKTA+++LN+L 
Sbjct: 466 FMIKEEVDLVFPLFDGAAETGRITRKAFTEWVVKVYTSRRALAHSLNDTKTAVKQLNKLV 525

Query: 535 SAFVLFVAIIVWLLVMGFLTMKVLVFISSQLLLVAFMFGNTAKTVFEAIIFVFVMHPFDV 594
           +A ++ V +++WLL++   T KVL+F S+QL+ +AF+ G+T K +FE+I+FVFVMHP+DV
Sbjct: 526 TAILMVVTVVIWLLLLEVATTKVLLFFSTQLVALAFIIGSTCKNLFESIVFVFVMHPYDV 585

Query: 595 GDRCVVDGVQMIVEEMNLLTTVFLKYDNEMIIYPNSVLATKPISNFYRSPPKMIDLVEFT 654
           GDRCVVDGV M+VEEMNLLTTVFLK +NE + YPN+VLATKPISN++RS P M + VEF+
Sbjct: 586 GDRCVVDGVAMLVEEMNLLTTVFLKLNNEKVYYPNAVLATKPISNYFRS-PNMGETVEFS 645

Query: 655 VDFSTSIKSIEDLKAGIKSYIAKNPKIWSSNHSFLVKDIENVDKMKMALYVNHTMNFQEY 714
           + FST +  I  LK  I  Y+ +NP+ W+  HS +VK+IEN++K+KMALY +HT+ FQE 
Sbjct: 646 ISFSTPVSKIAHLKERIAEYLEQNPQHWAPVHSVVVKEIENMNKLKMALYSDHTITFQEN 705

Query: 715 TLKSNRRSELVLELKRIFENLGIKYHLLPQQVQL 721
             ++ RR+EL L +KR+ E+L I Y LLPQ + L
Sbjct: 706 RERNLRRTELSLAIKRMLEDLHIDYTLLPQDINL 730

BLAST of Tan0004158 vs. TAIR 10
Match: AT5G19520.1 (mechanosensitive channel of small conductance-like 9 )

HSP 1 Score: 513.5 bits (1321), Expect = 2.9e-145
Identity = 330/753 (43.82%), Postives = 461/753 (61.22%), Query Frame = 0

Query: 8   EQVVVPILDSEGIVESSEERSVASFPDLEINDT----QSFRCTIPLSVVETSPSHEIVRM 67
           E+VV+ + D E   +S + R+  SF  L   D+    +  +   P+S+    P+ EI + 
Sbjct: 10  EEVVINVSDKE---DSKDPRASPSFNPLASPDSDAGIEKSKPVPPISI----PTPEIYKF 69

Query: 68  SPL--KPPKI-----------------SMPKS---------YPAC------ASLAEEKAQ 127
           S    KPPKI                 S PKS         Y +        SL E+   
Sbjct: 70  SGSVHKPPKIPSPEGLVRRKSLSRSIYSKPKSRFGEQQSFRYDSTREENGGRSLREQFGA 129

Query: 128 AKSTSGSLFCSSPKITTVTSPRDALQSAPRTPKGSGGEEDDNEEDYKTAEQKVKERSRKK 187
                GS   +SP   +  S   A  S     K +  E D+NEE YK    KVK    K+
Sbjct: 130 GSFARGSFDRASPNNKSNRSVASAALS-----KVAEEEPDENEEIYK----KVKLHRVKR 189

Query: 188 SKIPIL--IELGAFLCITGCLIASLTIDKLLTKEIWGLGLWRWCVLILVIFSGRLFSQWF 247
           S +  L  +EL  F+ I G LI SLTID +    IWGL  W+WCVL++V  SG L + WF
Sbjct: 190 SGMKPLAFLELVVFMAILGALIVSLTIDVVNKHTIWGLEFWKWCVLVMVTLSGMLVTNWF 249

Query: 248 IKCLVFLFERNFIFQEKVLYFIYGLRKSVIFFIWLGLILLAWVLLFNQSSDRSKKDKEIL 307
           +  +VF+ E+N++ ++KVLYF++GL+K+V  FIW  L+L+AW+ LF+    R++K K  L
Sbjct: 250 MHFVVFIIEKNYLLRKKVLYFVHGLKKNVQVFIWFSLVLIAWICLFDGDVKRTRKTKRFL 309

Query: 308 NYITRALGGSIIGATLWLVKTLLVKIVAASFQCAKFFERIKESFFHNYILQTLSRPPLMT 367
           ++IT  +   ++G+ L+LVKT  +K++A+ F    FFERI+ES FH Y+LQTLS PPL+ 
Sbjct: 310 DFITWTIVSLLVGSILFLVKTFALKVLASKFNVRNFFERIQESVFHQYVLQTLSGPPLIE 369

Query: 368 MTERVRRVASTGHLCYFKFLKKERDGKKEGKKGGIDVEKLRKMKQEEISAWTMSGLIDDI 427
             E V RV STGHL +     + +DGK + KK  ID+ K+ +MKQE++SAWTM  LI+ +
Sbjct: 370 EAENVGRVPSTGHLSF----TRTKDGKVKDKK-VIDMGKVHRMKQEKVSAWTMRVLIEAV 429

Query: 428 RNSGLSTISDTIENFNEEEAEQKDKEIKNEWEAKAAANQIFRNVAKAGSKFIEEEDLFPF 487
             SG+STIS T++  N ++ E+ DKEI NE EA AAA  +F NVAK    +IEE+DL  F
Sbjct: 430 GTSGISTISSTLDEVNNKK-ERTDKEITNEMEAVAAAYDVFNNVAKPNHNYIEEDDLLRF 489

Query: 488 MRKEVVNNVLKLFEGAVENRRIKRKTLKNWLVKVYQERKSLAQSLNDTKTAIEELNRLAS 547
           M KE V+ VL L E A +  +I RKT   W+V VY  RK++  SLNDTKTA+++L++L +
Sbjct: 490 MIKEEVDLVLPLIEDA-DTGKITRKTFTEWVVNVYTSRKTIGHSLNDTKTAVKQLDKLIT 549

Query: 548 AFVLFVAIIVWLLVMGFLTMKVLVFISSQLLLVAFMFGNTAKTVFEAIIFVFVMHPFDVG 607
             +  +  IVW++++   + K+L+  SSQ L +AFM G+T K +FE+ +FVFVMHP+DVG
Sbjct: 550 GILTVITFIVWMVLLDIASTKLLLVFSSQFLGLAFMIGSTCKNIFESFMFVFVMHPYDVG 609

Query: 608 DRCVVDGVQMIVEEMNLLTTVFLKYDNEMIIYPNSVLATKPISNFYRSPPKMIDLVEFTV 667
           DRCVVDGV ++VEE++LLTTVFLK DNE + YPNSVL +KPISNFYRS P M D V+F +
Sbjct: 610 DRCVVDGVMLLVEEIDLLTTVFLKIDNEKVFYPNSVLISKPISNFYRS-PDMGDYVDFGI 669

Query: 668 DFSTSIKSIEDLKAGIKSYIAKNPKIWSSNHSFLVKDIENVDKMKMALYVNHTMNFQEYT 721
            FST  + I  LK  I  Y+  N + W      +V+ IEN++K+ + + V HT+NFQ Y 
Sbjct: 670 AFSTPAEKIGCLKGKIGEYLVANSQHWYPEAQVMVRAIENMNKLVLNILVQHTINFQVYV 729

BLAST of Tan0004158 vs. TAIR 10
Match: AT3G14810.1 (mechanosensitive channel of small conductance-like 5 )

HSP 1 Score: 453.8 bits (1166), Expect = 2.7e-127
Identity = 267/636 (41.98%), Postives = 399/636 (62.74%), Query Frame = 0

Query: 123 GSGGEEDDNEEDYKTAEQKVKERSRKKSKIPILIELGAFLCITGCLIASLTIDKLLTKEI 182
           G  G E++ EED    E   +E  R K    + +E  + + I   L+ SLTI  L  K  
Sbjct: 238 GRNGFEEEEEEDPFLDEDLPEEFKRDKLSFWVFLEWISLVLIVTSLVCSLTIHNLQRKTW 297

Query: 183 WGLGLWRWCVLILVIFSGRLFSQWFIKCLVFLFERNFIFQEKVLYFIYGLRKSVIFFIWL 242
           W L LW+W V +LV+  GRL S W ++ +VFL E+NF ++++VLYF+YG+RKSV   +WL
Sbjct: 298 WKLDLWKWEVTVLVLICGRLVSSWIVRIIVFLVEKNFTWRKRVLYFVYGVRKSVQNCLWL 357

Query: 243 GLILLAWVLLFNQSSDRSKKDKEILNYITRALGGSIIGATLWLVKTLLVKIVAASFQCAK 302
           GL+LLAW  LF++  +R  +    L Y+TR L   ++   +WLVKT+LVK++A+SF  + 
Sbjct: 358 GLVLLAWHFLFDKKVERETR-STALRYVTRVLVCLLVALIIWLVKTILVKVLASSFHMST 417

Query: 303 FFERIKESFFHNYILQTLSRPPLM---TMTERVRRVA----STGHLCYFK---------- 362
           +F+RI+ES F  Y+++TLS PPLM    M E  ++VA    S   L   K          
Sbjct: 418 YFDRIQESLFTQYVIETLSGPPLMEIQRMEEEEQQVAEDVKSLEKLAGAKLPPALKATVK 477

Query: 363 -FLK------KERDGKKEGKKG-GIDVEKLRKMKQEEISAWTMSGLIDDIRNSGLSTISD 422
            F+K        R G K G+ G GI +++L++M  + +SAW M  L++ I    +ST+  
Sbjct: 478 SFMKVGKSPGLNRIGSKRGEDGEGIRIDQLKRMNTKNVSAWNMKRLMNIILKGAISTLDQ 537

Query: 423 TIENFNEEEAEQKDKEIKNEWEAKAAANQIFRNVAKAGSKFIEEEDLFPFMRKEVVNNVL 482
            +++  +E+ +     I++E+EAK AA +IF NV + GS++I  ED   F+ +E     +
Sbjct: 538 NMQDTTQEDEDA--THIRSEYEAKCAARKIFHNVTEPGSRYIYLEDFLRFLCEEEAERAM 597

Query: 483 KLFEGAVENRRIKRKTLKNWLVKVYQERKSLAQSLNDTKTAIEELNRLASAFVLFVAIIV 542
            LFEGA E+ +I +  LKNW+VK ++ER++LA +LNDTKTA++ L+R+ +  +  + II+
Sbjct: 598 ALFEGASESDKISKSCLKNWVVKAFRERRALALTLNDTKTAVDRLHRIINVVIGIIIIII 657

Query: 543 WLLVMGFLTMKVLVFISSQLLLVAFMFGNTAKTVFEAIIFVFVMHPFDVGDRCVVDGVQM 602
           WLL++G  T + L+ +SSQLLLVAF+FGN+ KT+FEAIIF+FVMHPFDVGDRC +DGVQ+
Sbjct: 658 WLLILGIATTRFLLVLSSQLLLVAFVFGNSCKTIFEAIIFLFVMHPFDVGDRCEIDGVQL 717

Query: 603 IVEEMNLLTTVFLKYDNEMIIYPNSVLATKPISNFYRSPPKMIDLVEFTVDFSTSIKSIE 662
           +VEEMN+LTTVFL+YDN+ IIYPNSVL TKPI+N+YRS P M D VEF V  +T  + I 
Sbjct: 718 VVEEMNILTTVFLRYDNQKIIYPNSVLGTKPIANYYRS-PDMGDAVEFCVHIATPPEKIT 777

Query: 663 DLKAGIKSYIAKNPKIWSSNHSFLVKDIENVDKMKMALYVNHTMNFQEYTLKSNRRSELV 722
            +K  I SY+      W      +   +++++ +K+A+++ H MN Q+   +  RR  L+
Sbjct: 778 AIKQRILSYVDNKKDYWYPAPMIVFLSMDDLNSVKIAVWLTHRMNHQDMGERYIRRGLLL 837

Query: 723 LELKRIFENLGIKYHLLPQQVQLNYGSSPALPPSRN 734
            E+ +    L I+Y L P  + +      +LPP+ N
Sbjct: 838 EEVGKTCRELDIEYRLYPLNINVR-----SLPPTAN 864