Tan0003944 (gene) Snake gourd v1

BLAST of Tan0003944 vs. ExPASy Swiss-Prot
Match: Q9FI21 (Berberine bridge enzyme-like 28 OS=Arabidopsis thaliana OX=3702 GN=At5g44440 PE=1 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 1.5e-143
Identity = 251/515 (48.74%), Postives = 358/515 (69.51%), Query Frame = 0

Query: 30  HQDFLQCLFDHSKDSYSITKVVYTPINSSYTSVLNFSIRNQRFSTAKTPKPLVIIRPSKV 89
           H+DFL+CL     D+    KV++T  +SS+ S+L+ SI+N RFS ++TPKP+ II P K 
Sbjct: 28  HEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSIQNPRFSVSETPKPVSIITPVKA 87

Query: 90  SHIQAAVICSKSHGLQIRIRSGGHDFEGLSYAAY-LPFVVVDLIALRAVTVDVKNSIAWV 149
           S +Q  + C++ HG+ +R RS GH +EGLSY AY  PF V+DL  LR++++DV N   WV
Sbjct: 88  SDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVIDLRNLRSISLDVDNRTGWV 147

Query: 150 QSGATLGELYYRIAEKSRTLAFPAGVCPSVGVGGQFSGGGYGLMLRKYGIAADNVIDAYL 209
           Q+GAT GELYY I + +++LAFPAG+ P+VGVGGQFSGGGYG +LRKYG+AADN+IDA +
Sbjct: 148 QTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGYGTLLRKYGLAADNIIDALV 207

Query: 210 VDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICTIYRTQKEDAI 269
           VDA+G+  DR++MGED FWAIRGGGG SFG++++WKVKLV VP+T+T+  + +T K++A+
Sbjct: 208 VDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVPSTITVFKVQKTSKKEAV 267

Query: 270 KLIHRWQYVANNLDENILLGIFLTGGNISTQRGGKINPRALFFSLFMGKTDELLAILKTT 329
           ++I +WQY A+ + +++ +         + +R  K    ALF  L++G  + LLA+++  
Sbjct: 268 RIIKKWQYAADKVPDDLFI-------RTTLERSNKNAVHALFTGLYIGPVNNLLALMEEK 327

Query: 330 FPELGFTKEECRETSWIESALIAANGFQIGETLEALLNRSALIPPISRGSSKIKSDYIKE 389
           FPELG  KE C E SWIES L  A+ F  GE+L  L NR       +  S K K D+++E
Sbjct: 328 FPELGLEKEGCEEMSWIESVLWFAD-FPKGESLGVLTNRER-----TSLSFKGKDDFVQE 387

Query: 390 PITEAAIEGIWERSKSQNIESTQVVFIPYGGRMSQISESEIPYSHRAAYLYKIGYIIGWQ 449
           PI EAAI+ IW R ++      +++  P+GG+MS+++E E P+ HR   LY+I Y+  W+
Sbjct: 388 PIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPFPHRGGNLYEIQYVAYWR 447

Query: 450 EQ----SVEAEKRHINWIRELYSYMTPFVSKSPRAAYVNYRDLDIGSNNKNGKTSYKKAM 509
           E+      E +K ++ W+  +Y +MTP+VSKSPR AYVN++D+D+G      KT Y++  
Sbjct: 448 EEEDKNKTETDK-YLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDLGMYLGKKKTKYEEGK 507

Query: 510 TWGLKYFGKNNFNRLVRVKTEVDPDNFFRHEQSIP 540
           +WG+KYF KNNF RLVRVKT VDP +FF  EQSIP
Sbjct: 508 SWGVKYF-KNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of Tan0003944 vs. ExPASy Swiss-Prot
Match: O64743 (Berberine bridge enzyme-like 15 OS=Arabidopsis thaliana OX=3702 GN=MEE23 PE=1 SV=1)

HSP 1 Score: 510.0 bits (1312), Expect = 3.3e-143
Identity = 260/539 (48.24%), Postives = 372/539 (69.02%), Query Frame = 0

Query: 6   LIPLSLAFVVLSSSSLWAMASYDKHQDFLQCLFDHSKDSYSITKVVYTPINSS--YTSVL 65
           L+ L L  V LSSS+L         QDF++CL D+S  S+ IT   ++P  ++  +   L
Sbjct: 14  LVTLLLISVPLSSSTL--------QQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEEL 73

Query: 66  NFSIRNQRFSTAKTPKPLVIIRPSKVSHIQAAVICSKSHGLQIRIRSGGHDFEGLSYAA- 125
             + +N R+ T   PKP+ I  P   +H+QAAV+C+K   L +R+RSGGHD+EGLS+ A 
Sbjct: 74  ESTAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAE 133

Query: 126 -YLPFVVVDLIALRAVTVDVKNSIAWVQSGATLGELYYRIAEKSRTLAFPAGVCPSVGVG 185
              PFV+VDL  LR V VD+ ++ AW  +GAT+GE+YYRI EKS+T  FPAG+C S+G+G
Sbjct: 134 DETPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIG 193

Query: 186 GQFSGGGYGLMLRKYGIAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVV 245
           G   GG YG M+RK+G+ ADNV+DA +VDANG+  DR +MGED+FWAIRGGGGGSFG+++
Sbjct: 194 GHLVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVIL 253

Query: 246 AWKVKLVPVPATVTICTIYRTQKEDAIKLIHRWQYVANNLDENILLGIFLTGGNISTQRG 305
           AWK+KLVPVPATVT+ T+ +T ++D  K++++W+ +A+ LD+++ + + ++  + +T+ G
Sbjct: 254 AWKIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPG 313

Query: 306 GKINPRALFFSLFMGKTDELLAILKTTFPELGFTKEECRETSWIESALIAANGFQIGETL 365
            +    + + + F+G ++ LL +++ +FPELG TK++C E SWI+S +  A GF      
Sbjct: 314 NRTISMS-YQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIA-GFPNSAAP 373

Query: 366 EALLNRSALIPPISRGSSKIKSDYIKEPITEAAIEGIWERSKSQNIESTQVVFIPYGGRM 425
           EALL   +L     +   K KSD++KEPI    +EG+WER   +  +S   ++ PYGG M
Sbjct: 374 EALLAGKSLF----KNHFKAKSDFVKEPIPVEGLEGLWERFLEE--DSPLTIWNPYGGMM 433

Query: 426 SQISESEIPYSHRAAYLYKIGYIIGWQEQSVEAEKRHINWIRELYSYMTPFVSKSPRAAY 485
           S+ISESEIP+ HR   L+KI ++  WQ+  V +E+RH+ WIRE+YSYM  +VSK+PR AY
Sbjct: 434 SRISESEIPFPHRNGTLFKIQWLSTWQDGKV-SEERHMKWIREMYSYMEQYVSKNPRQAY 493

Query: 486 VNYRDLDIGSNNKNGKTSYKKAMTWGLKYFGKNNFNRLVRVKTEVDPDNFFRHEQSIPT 541
           VNYRDLD+G+N   G+T    A  WG KY+ K NF RLV++K E DPDNFFRHEQS+PT
Sbjct: 494 VNYRDLDLGTN--EGETD---AREWGAKYY-KGNFERLVKIKGEFDPDNFFRHEQSVPT 529

BLAST of Tan0003944 vs. ExPASy Swiss-Prot
Match: A6P6W0 (Cannabidiolic acid synthase-like 1 OS=Cannabis sativa OX=3483 GN=CBDAS2 PE=2 SV=1)

HSP 1 Score: 507.7 bits (1306), Expect = 1.7e-142
Identity = 252/516 (48.84%), Postives = 353/516 (68.41%), Query Frame = 0

Query: 31  QDFLQCLFDHSKDSYSITKVVYTPINSSYTSVLNFSIRNQRFSTAKTPKPLVIIRPSKVS 90
           ++FL+C   +   + +  K+VYT  +  Y S+LN +I+N RF++  TPKPLVII P  VS
Sbjct: 32  ENFLKCFSQYIPTNVTNAKLVYTQHDQFYMSILNSTIQNLRFTSDTTPKPLVIITPLNVS 91

Query: 91  HIQAAVICSKSHGLQIRIRSGGHDFEGLSYAAYLPFVVVDLIALRAVTVDVKNSIAWVQS 150
           HIQ  ++CSK  GLQIR RSGGHD EG+SY + +PFV+VDL  + +V +DV +  AWV++
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVEA 151

Query: 151 GATLGELYYRIAEKSRTLAFPAGVCPSVGVGGQFSGGGYGLMLRKYGIAADNVIDAYLVD 210
           GATLGE+YY I E +  L+FPAG CP+VG GG FSGGGYG ++R YG+AADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGAGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 211 ANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICTIYRTQK-EDAIK 270
            +GK  DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  TI ++ +  +  + +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 271 LIHRWQYVANNLDENILLGIFLTGGNISTQRG-GKINPRALFFSLFMGKTDELLAILKTT 330
           L+++WQ +A   ++ +LL       NI+  +G  K    + F S+F G  D L+ ++  +
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 331 FPELGFTKEECRETSWIESALIAANGFQIGETL---EALLNRSALIPPISRGSSKIKSDY 390
           FPELG  K +C++ SWI++ +  +       T    E LL+RS       + +  IK DY
Sbjct: 332 FPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTYFKKEILLDRSG----GRKAAFSIKLDY 391

Query: 391 IKEPITEAAIEGIWERSKSQNIESTQVVFIPYGGRMSQISESEIPYSHRAAYLYKIGYII 450
           +K+PI E A+  I E+   +++     VF PYGG M +ISES IP+ HRA  +Y+I YI 
Sbjct: 392 VKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGIMYEIWYIA 451

Query: 451 GWQEQSVEAEKRHINWIRELYSYMTPFVSKSPRAAYVNYRDLDIGSNNKNGKTSYKKAMT 510
            W++Q  E  ++HINWIR +Y++ TP+VS++PR AY+NYRDLD+G  N     +Y +A  
Sbjct: 452 SWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARI 511

Query: 511 WGLKYFGKNNFNRLVRVKTEVDPDNFFRHEQSIPTL 542
           WG KYFGK NFNRLV+VKT+VDPDNFFR+EQSIP L
Sbjct: 512 WGEKYFGK-NFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Tan0003944 vs. ExPASy Swiss-Prot
Match: A6P6W1 (Cannabidiolic acid synthase-like 2 OS=Cannabis sativa OX=3483 GN=CBDAS3 PE=2 SV=1)

HSP 1 Score: 506.5 bits (1303), Expect = 3.7e-142
Identity = 252/516 (48.84%), Postives = 352/516 (68.22%), Query Frame = 0

Query: 31  QDFLQCLFDHSKDSYSITKVVYTPINSSYTSVLNFSIRNQRFSTAKTPKPLVIIRPSKVS 90
           ++FL+CL  +   + +  K+VYT  +  Y S+LN +++N RF++  TPKPLVI  P  VS
Sbjct: 32  ENFLKCLSQYIPTNVTNAKLVYTQHDQFYMSILNSTVQNLRFTSDTTPKPLVITTPLNVS 91

Query: 91  HIQAAVICSKSHGLQIRIRSGGHDFEGLSYAAYLPFVVVDLIALRAVTVDVKNSIAWVQS 150
           HIQ  ++CSK  GLQIR RSGGHD EG+SY + +PFV+VDL  + +V +DV +  AWV+S
Sbjct: 92  HIQGTILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVIVDLRNMHSVKIDVHSQTAWVES 151

Query: 151 GATLGELYYRIAEKSRTLAFPAGVCPSVGVGGQFSGGGYGLMLRKYGIAADNVIDAYLVD 210
           GATLGE+YY I E +  L+FPAG CP+VG GG FSGGGYG ++R YG+AADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINENNENLSFPAGYCPTVGTGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 211 ANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICTIYRTQK-EDAIK 270
            +GK  DR+SMGEDLFWAIRGGGG +FGI+ AWK++LV VP+  TI ++ +  +  + +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIRLVAVPSMSTIFSVKKNMEIHELVK 271

Query: 271 LIHRWQYVANNLDENILLGIFLTGGNISTQRG-GKINPRALFFSLFMGKTDELLAILKTT 330
           L+++WQ +A   ++ +LL       NI+  +G  K    + F S+F G  D L+ ++  +
Sbjct: 272 LVNKWQNIAYMYEKELLLFTHFITRNITDNQGKNKTTIHSYFSSIFHGGVDSLVDLMNKS 331

Query: 331 FPELGFTKEECRETSWIESALIAANGFQIGET---LEALLNRSALIPPISRGSSKIKSDY 390
           FPELG  K +C++ SWI++ +  +       T    E LL+RS       + +  IK DY
Sbjct: 332 FPELGIKKTDCKQLSWIDTIIFYSGVVNYNTTNFKKEILLDRSG----GRKAAFSIKLDY 391

Query: 391 IKEPITEAAIEGIWERSKSQNIESTQVVFIPYGGRMSQISESEIPYSHRAAYLYKIGYII 450
           +K+PI E A+  I E+   +++     VF PYGG M +ISES IP+ HRA   Y+I YI 
Sbjct: 392 VKKPIPETAMVTILEKLYEEDVGVGMFVFYPYGGIMDEISESAIPFPHRAGITYEIWYIA 451

Query: 451 GWQEQSVEAEKRHINWIRELYSYMTPFVSKSPRAAYVNYRDLDIGSNNKNGKTSYKKAMT 510
            W++Q  E  ++HINWIR +Y++ TP+VS++PR AY+NYRDLD+G  N     +Y +A  
Sbjct: 452 SWEKQ--EDNEKHINWIRNVYNFTTPYVSQNPRMAYLNYRDLDLGKTNFESPNNYTQARI 511

Query: 511 WGLKYFGKNNFNRLVRVKTEVDPDNFFRHEQSIPTL 542
           WG KYFGK NFNRLV+VKT+VDPDNFFR+EQSIP L
Sbjct: 512 WGEKYFGK-NFNRLVKVKTKVDPDNFFRNEQSIPPL 540

BLAST of Tan0003944 vs. ExPASy Swiss-Prot
Match: Q8GTB6 (Tetrahydrocannabinolic acid synthase OS=Cannabis sativa OX=3483 GN=THCAS PE=1 SV=1)

HSP 1 Score: 505.8 bits (1301), Expect = 6.3e-142
Identity = 252/516 (48.84%), Postives = 351/516 (68.02%), Query Frame = 0

Query: 31  QDFLQCLFDHSKDSYSITKVVYTPINSSYTSVLNFSIRNQRFSTAKTPKPLVIIRPSKVS 90
           ++FL+C   H  ++ +  K+VYT  +  Y S+LN +I+N RF +  TPKPLVI+ PS  S
Sbjct: 32  ENFLKCFSKHIPNNVANPKLVYTQHDQLYMSILNSTIQNLRFISDTTPKPLVIVTPSNNS 91

Query: 91  HIQAAVICSKSHGLQIRIRSGGHDFEGLSYAAYLPFVVVDLIALRAVTVDVKNSIAWVQS 150
           HIQA ++CSK  GLQIR RSGGHD EG+SY + +PFVVVDL  + ++ +DV +  AWV++
Sbjct: 92  HIQATILCSKKVGLQIRTRSGGHDAEGMSYISQVPFVVVDLRNMHSIKIDVHSQTAWVEA 151

Query: 151 GATLGELYYRIAEKSRTLAFPAGVCPSVGVGGQFSGGGYGLMLRKYGIAADNVIDAYLVD 210
           GATLGE+YY I EK+  L+FP G CP+VGVGG FSGGGYG ++R YG+AADN+IDA+LV+
Sbjct: 152 GATLGEVYYWINEKNENLSFPGGYCPTVGVGGHFSGGGYGALMRNYGLAADNIIDAHLVN 211

Query: 211 ANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICTIYRTQK-EDAIK 270
            +GK  DR+SMGEDLFWAIRGGGG +FGI+ AWK+KLV VP+  TI ++ +  +    +K
Sbjct: 212 VDGKVLDRKSMGEDLFWAIRGGGGENFGIIAAWKIKLVAVPSKSTIFSVKKNMEIHGLVK 271

Query: 271 LIHRWQYVANNLDENILLGIFLTGGNISTQRG-GKINPRALFFSLFMGKTDELLAILKTT 330
           L ++WQ +A   D++++L       NI+   G  K      F S+F G  D L+ ++  +
Sbjct: 272 LFNKWQNIAYKYDKDLVLMTHFITKNITDNHGKNKTTVHGYFSSIFHGGVDSLVDLMNKS 331

Query: 331 FPELGFTKEECRETSWIESALI---AANGFQIGETLEALLNRSALIPPISRGSSKIKSDY 390
           FPELG  K +C+E SWI++ +      N        E LL+RSA      + +  IK DY
Sbjct: 332 FPELGIKKTDCKEFSWIDTTIFYSGVVNFNTANFKKEILLDRSA----GKKTAFSIKLDY 391

Query: 391 IKEPITEAAIEGIWERSKSQNIESTQVVFIPYGGRMSQISESEIPYSHRAAYLYKIGYII 450
           +K+PI E A+  I E+   +++ +   V  PYGG M +ISES IP+ HRA  +Y++ Y  
Sbjct: 392 VKKPIPETAMVKILEKLYEEDVGAGMYVLYPYGGIMEEISESAIPFPHRAGIMYELWYTA 451

Query: 451 GWQEQSVEAEKRHINWIRELYSYMTPFVSKSPRAAYVNYRDLDIGSNNKNGKTSYKKAMT 510
            W++Q  E  ++HINW+R +Y++ TP+VS++PR AY+NYRDLD+G  N     +Y +A  
Sbjct: 452 SWEKQ--EDNEKHINWVRSVYNFTTPYVSQNPRLAYLNYRDLDLGKTNHASPNNYTQARI 511

Query: 511 WGLKYFGKNNFNRLVRVKTEVDPDNFFRHEQSIPTL 542
           WG KYFGK NFNRLV+VKT+VDP+NFFR+EQSIP L
Sbjct: 512 WGEKYFGK-NFNRLVKVKTKVDPNNFFRNEQSIPPL 540

BLAST of Tan0003944 vs. NCBI nr
Match: XP_022152516.1 (berberine bridge enzyme-like 18 [Momordica charantia])

HSP 1 Score: 829.3 bits (2141), Expect = 1.9e-236
Identity = 408/543 (75.14%), Postives = 466/543 (85.82%), Query Frame = 0

Query: 3   SSPLIPL--SLAFVVLSSSSLWAMASYDKHQDFLQCLFDHSKDS-YSITKVVYTPINSSY 62
           SSPLIPL  SL F+ LSSSSLWA AS DKH+ FL CL DHS DS YSI+KVVYTP+NSSY
Sbjct: 4   SSPLIPLSFSLLFIFLSSSSLWAAASVDKHESFLNCLLDHSPDSNYSISKVVYTPLNSSY 63

Query: 63  TSVLNFSIRNQRFSTAKTPKPLVIIRPSKVSHIQAAVICSKSHGLQIRIRSGGHDFEGLS 122
           +SVL+FSIRN RFSTA TPKPL II P  VSH+QAAV+CS++HGLQIR RSGGHDFEGLS
Sbjct: 64  SSVLDFSIRNLRFSTADTPKPLFIITPLHVSHVQAAVVCSRTHGLQIRTRSGGHDFEGLS 123

Query: 123 YAAYLPFVVVDLIALRAVTVDVKNSIAWVQSGATLGELYYRIAEKSRTLAFPAGVCPSVG 182
             ++LPF+++DLI LR++T+D  N+ AWVQSGATLGELYY IAEKSRTLAFPAG CP+VG
Sbjct: 124 CVSHLPFIIIDLINLRSITIDANNNTAWVQSGATLGELYYSIAEKSRTLAFPAGACPTVG 183

Query: 183 VGGQFSGGGYGLMLRKYGIAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGI 242
           +GG FSGGGYGLMLRK+G+AADNVIDAYLVDANGKF DRE MGEDLFWAIRGGGGGSFGI
Sbjct: 184 IGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKFQDRELMGEDLFWAIRGGGGGSFGI 243

Query: 243 VVAWKVKLVPVPATVTICTIYRTQKEDAIKLIHRWQYVANNLDENILLGIFLTGGNISTQ 302
           VVAWKVKLVPVPATVT C   RT +E AIKLIHRWQYVAN LDEN+ LGI L GGN + Q
Sbjct: 244 VVAWKVKLVPVPATVTSCYYNRTLEEGAIKLIHRWQYVANKLDENLFLGILLMGGNFTAQ 303

Query: 303 RGGKINPRALFFSLFMGKTDELLAILKTTFPELGFTKEECRETSWIESALIAANGFQIGE 362
            G K NP A FFSLF+GK DE  AIL TTFPELG  KE C E+SWIESAL++A GF+IG+
Sbjct: 304 GGDKTNPTASFFSLFLGKADEFFAILNTTFPELGLAKENCLESSWIESALLSA-GFKIGD 363

Query: 363 TLEALLNRSALIPPISRGSSKIKSDYIKEPITEAAIEGIWERSKSQNIESTQVVFIPYGG 422
           +LEALLNR+    P++ GS KIKSDY+KEPI+EA IEGIWER KS+++ + Q++F+PYGG
Sbjct: 364 SLEALLNRT----PLTNGSIKIKSDYVKEPISEATIEGIWERLKSRDVGARQLLFVPYGG 423

Query: 423 RMSQISESEIPYSHRAAYLYKIGYIIGWQEQSVEAEKRHINWIRELYSYMTPFVSKSPRA 482
           RMSQISESEIP+SHRA  LYKIGYI+ WQ+Q+V+ EKRH+ WIRELYSYMTPFVSKSPR+
Sbjct: 424 RMSQISESEIPFSHRAGNLYKIGYIVAWQKQNVDVEKRHLKWIRELYSYMTPFVSKSPRS 483

Query: 483 AYVNYRDLDIGSNNKNGKTSYKKAMTWGLKYFGKNNFNRLVRVKTEVDPDNFFRHEQSIP 542
           AYVNYRDLDIGSNNK GKTS+K+A  WG KYFG NNFNRLV VKT+VDP +FFRHEQSIP
Sbjct: 484 AYVNYRDLDIGSNNKYGKTSFKRASVWGWKYFG-NNFNRLVHVKTKVDPYDFFRHEQSIP 540

BLAST of Tan0003944 vs. NCBI nr
Match: XP_038895343.1 (cannabidiolic acid synthase-like [Benincasa hispida])

HSP 1 Score: 823.2 bits (2125), Expect = 1.4e-234
Identity = 414/544 (76.10%), Postives = 460/544 (84.56%), Query Frame = 0

Query: 1   MKSSPLIPLSLAFVVLSSSSLWAMA---SYDKHQDFLQCLFDHSKDSYSITKVVYTPINS 60
           MK SPLIPLSLAF++L SSS    A   SY K+++FLQCL  HS ++YSIT +VY P NS
Sbjct: 1   MKFSPLIPLSLAFIILCSSSSLLSATSSSYIKYEEFLQCLLHHSPNTYSITNLVYAPTNS 60

Query: 61  SYTSVLNFSIRNQRFSTAKTPKPLVIIRPSKVSHIQAAVICSKSHGLQIRIRSGGHDFEG 120
           SY+S+LNFSIRN RFST   PKPL+II PS +SHIQAAV+CSKSHGLQIRIRSGGHDFEG
Sbjct: 61  SYSSLLNFSIRNLRFSTPTIPKPLLIITPSNISHIQAAVVCSKSHGLQIRIRSGGHDFEG 120

Query: 121 LSYAAYLPFVVVDLIALRAVTVDVKNSIAWVQSGATLGELYYRIAEKSRTLAFPAGVCPS 180
           LSY AY PF+VVDLI L +V VDV+ S AWVQSGATLGELYYRIAEKSRTLAFPAG CP+
Sbjct: 121 LSYVAYHPFIVVDLINLSSVAVDVEQSTAWVQSGATLGELYYRIAEKSRTLAFPAGTCPT 180

Query: 181 VGVGGQFSGGGYGLMLRKYGIAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSF 240
           VGVGG FSGGGYG +LRKYG+AADNVIDAYLVDANG+FHDRESMGEDLFWAIRGGGGGSF
Sbjct: 181 VGVGGHFSGGGYGTLLRKYGLAADNVIDAYLVDANGEFHDRESMGEDLFWAIRGGGGGSF 240

Query: 241 GIVVAWKVKLVPVPATVTICTIYRTQKEDAIKLIHRWQYVANNLDENILLGIFLTGGNIS 300
           GIVVAWKVKLVPVPATVTICT  RT KE AIKLI +WQYVAN LDE++ L I LTGG IS
Sbjct: 241 GIVVAWKVKLVPVPATVTICTANRTWKEGAIKLIDQWQYVANKLDEDLFLSIILTGGKIS 300

Query: 301 TQRGGKINPRALFFSLFMGKTDELLAILKTTFPELGFTKEECRETSWIESALIAANGFQI 360
            Q GGKINP ALFF+LF+GK DELL ILK  FPEL   KE+C+ETSWIESA+  ANG QI
Sbjct: 301 AQ-GGKINPTALFFTLFLGKADELLTILKNKFPELNLAKEDCKETSWIESAVYTANGLQI 360

Query: 361 -GETLEALLNRSALIPPISRGSSKIKSDYIKEPITEAAIEGIWERSKSQNIESTQVVFIP 420
             + LEA LNR+    P++ GS KIKSDY+KEPIT+AAIEGIW+R KSQ+IE   +  IP
Sbjct: 361 DDQPLEAFLNRT----PLANGSFKIKSDYVKEPITKAAIEGIWQRLKSQDIEGVNLGLIP 420

Query: 421 YGGRMSQISESEIPYSHRAAYLYKIGYIIGWQEQSVEAEKRHINWIRELYSYMTPFVSKS 480
           YGGRMSQISESEIP+ HR   LYKIGYIIGW+EQ V+AEKRH+NWIRE+YSYMTPFVSKS
Sbjct: 421 YGGRMSQISESEIPFPHRIGNLYKIGYIIGWEEQGVDAEKRHLNWIREIYSYMTPFVSKS 480

Query: 481 PRAAYVNYRDLDIGSNNKNGKTSYKKAMTWGLKYFGKNNFNRLVRVKTEVDPDNFFRHEQ 540
           PRAAYVNYRDLDIGSN+K GKTSYK+A  WG KYFG NNFNRLV VK EVDP +FFRHEQ
Sbjct: 481 PRAAYVNYRDLDIGSNDKYGKTSYKRACIWGKKYFG-NNFNRLVHVKNEVDPYDFFRHEQ 538

BLAST of Tan0003944 vs. NCBI nr
Match: XP_023000935.1 (berberine bridge enzyme-like 27 [Cucurbita maxima])

HSP 1 Score: 819.7 bits (2116), Expect = 1.5e-233
Identity = 400/542 (73.80%), Postives = 459/542 (84.69%), Query Frame = 0

Query: 1   MKSSPLIPLSLAFVVLSSSSLWAMASYDKHQDFLQCLFDHSKDSYSITKVVYTPINSSYT 60
           MKSSPL  LSL F+VL SSSLWA  S ++++DFLQCL  HS D++SITK+VYTPIN+SY+
Sbjct: 1   MKSSPLTLLSLTFIVLCSSSLWAAPSSNQNKDFLQCLLHHSPDTHSITKLVYTPINASYS 60

Query: 61  SVLNFSIRNQRFSTAKTPKPLVIIRPSKVSHIQAAVICSKSHGLQIRIRSGGHDFEGLSY 120
           S+LNFSI+N RFST KTPKPL II PS + HIQAA+ICSKSHGLQIRIRSGGHD EGLSY
Sbjct: 61  SLLNFSIKNLRFSTPKTPKPLAIITPSNIHHIQAAIICSKSHGLQIRIRSGGHDLEGLSY 120

Query: 121 AAYLPFVVVDLIALRAVTVDVKNSIAWVQSGATLGELYYRIAEKSRTLAFPAGVCPSVGV 180
            AY+PF+VVDLI L  V VDV+ + AWVQSGA+LGELYYRIAEKSRTLAFPAG+CP+VGV
Sbjct: 121 VAYVPFIVVDLINLSLVEVDVEKNTAWVQSGASLGELYYRIAEKSRTLAFPAGLCPTVGV 180

Query: 181 GGQFSGGGYGLMLRKYGIAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIV 240
           GGQFSGGGYG+++RKYG+AADNV+DAYLVDANG+F DRE MGE+LFWAIRGGGGGSFG+V
Sbjct: 181 GGQFSGGGYGMLMRKYGLAADNVVDAYLVDANGEFFDREGMGEELFWAIRGGGGGSFGVV 240

Query: 241 VAWKVKLVPVPATVTICTIYRTQKEDAIKLIHRWQYVANNLDENILLGIFLTGGNISTQR 300
           VAWKVKLVPVPATVT+C   ++  + AI LI +WQYVA+ LDEN+ LGI L G       
Sbjct: 241 VAWKVKLVPVPATVTVCATSKSLVDGAINLIDKWQYVADKLDENLFLGIILAG------- 300

Query: 301 GGKINPRALFFSLFMGKTDELLAILKTTFPELGFTKEECRETSWIESALIAANGFQIGET 360
           GGK NP A+FFSLF+GK DEL+ IL   FPELG +KE+C ETSWIE+ALIAANGFQ GE 
Sbjct: 301 GGKTNPLAIFFSLFLGKADELVTILNNPFPELGLSKEDCIETSWIEAALIAANGFQTGEV 360

Query: 361 LEALLNRSALIPPISRGSSKIKSDYIKEPITEAAIEGIWERSKSQNIESTQVVFIPYGGR 420
           LEALLNRSALIPPI+ G SK KSDY+KEP+T  AIEG+W RSKSQ IE   +VFIPYGG+
Sbjct: 361 LEALLNRSALIPPINSGRSKTKSDYVKEPMTRTAIEGMWNRSKSQEIERASLVFIPYGGK 420

Query: 421 MSQISESEIPYSHRAAYLYKIGYIIGWQEQSVEAEKRHINWIRELYSYMTPFVSKSPRAA 480
           MS+ISESEIP+ HRA   Y+I YI+GW EQ V AEKRH+NWIRELYSYMTPFVSKSPRA 
Sbjct: 421 MSEISESEIPFPHRAGNRYEIDYIVGWDEQGVHAEKRHLNWIRELYSYMTPFVSKSPRAV 480

Query: 481 YVNYRDLDIGSNNKNGKTSYKKAMTWGLKYFGKNNFNRLVRVKTEVDPDNFFRHEQSIPT 540
           YVN RDLDIGSN+  G+TSYK+A  WGLKYFG NNF+RLV VKTEVDP +FFRHEQSIPT
Sbjct: 481 YVNCRDLDIGSNDMYGRTSYKRARVWGLKYFG-NNFDRLVHVKTEVDPYDFFRHEQSIPT 534

Query: 541 LT 543
           L+
Sbjct: 541 LS 534

BLAST of Tan0003944 vs. NCBI nr
Match: XP_022142587.1 (tetrahydrocannabinolic acid synthase-like [Momordica charantia])

HSP 1 Score: 818.9 bits (2114), Expect = 2.6e-233
Identity = 396/540 (73.33%), Postives = 463/540 (85.74%), Query Frame = 0

Query: 4   SPLIPLSLAFVVLSSSSLWAMASYDKHQDFLQCLFDHSKD--SYSITKVVYTPINSSYTS 63
           SPLI LSLAF+ LSSSSLWA A  +KH+ FLQCL DHS +  +YSI+KV++TPINSSY+S
Sbjct: 5   SPLISLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINSSYSS 64

Query: 64  VLNFSIRNQRFSTAKTPKPLVIIRPSKVSHIQAAVICSKSHGLQIRIRSGGHDFEGLSYA 123
           VL+FSIRN RFST +TPKPL+I+ PS VSHIQAAVICSKS+G QIR RSGGHDFEGLSY 
Sbjct: 65  VLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLSYV 124

Query: 124 AYLPFVVVDLIALRAVTVDVKNSIAWVQSGATLGELYYRIAEKSRTLAFPAGVCPSVGVG 183
           A+LPF++VDLI LR++++D+K++ AWVQSGATLGELYY IA+KSRTLAFPAG+CP+VGVG
Sbjct: 125 AHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVGVG 184

Query: 184 GQFSGGGYGLMLRKYGIAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVV 243
           G FSGGGYGL+LRKYG+AADNVIDAYLVDA+GKFH+RESMGEDLFWAIRGGGGGSFGIVV
Sbjct: 185 GHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGIVV 244

Query: 244 AWKVKLVPVPATVTICTIYRTQKEDAIKLIHRWQYVANNLDENILLGIFLTGGNISTQRG 303
           AWKVKLVPVPATVT+C+  RT +E A+K+ HRWQYVAN LDEN+ LGI   GG I++Q G
Sbjct: 245 AWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKITSQEG 304

Query: 304 GKINPRALFFSLFMGKTDELLAILKTTFPELGFTKEECRETSWIESALIAANGFQIGETL 363
           GK NP A FFSLF+GK DELL+IL T FPEL   K++C ETSWIES ++   GF    TL
Sbjct: 305 GKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPTELTL 364

Query: 364 EALLNRSALIPPISRGSSKIKSDYIKEPITEAAIEGIWERSKSQNIESTQVVFIPYGGRM 423
           EALL+R+    P++  SSKIKSDY+KEP++EA ++GIW+R  SQ+IE   ++F+PYGGRM
Sbjct: 365 EALLSRT----PLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRM 424

Query: 424 SQISESEIPYSHRAAYLYKIGYIIGWQEQSVEAEKRHINWIRELYSYMTPFVSKSPRAAY 483
           SQISESE P+SHRA YLYKIGYI GW+EQ ++AEKRH+ WIRELY YMTPFVSKSPRAAY
Sbjct: 425 SQISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAY 484

Query: 484 VNYRDLDIGSNNKNGKTSYKKAMTWGLKYFGKNNFNRLVRVKTEVDPDNFFRHEQSIPTL 542
            NYRDLDIGSNNK GKTSYK+A  WGLKYFG NNFNRLV VKT+VDP +FFRHEQSIP L
Sbjct: 485 ANYRDLDIGSNNKFGKTSYKQASVWGLKYFG-NNFNRLVHVKTKVDPYDFFRHEQSIPAL 539

BLAST of Tan0003944 vs. NCBI nr
Match: XP_038895508.1 (berberine bridge enzyme-like 28 [Benincasa hispida])

HSP 1 Score: 815.5 bits (2105), Expect = 2.8e-232
Identity = 404/539 (74.95%), Postives = 462/539 (85.71%), Query Frame = 0

Query: 3   SSPLIPLSLAFVVLSSSSLWAMASYDKHQDFLQCLFDHSKDSYSITKVVYTPINSSYTSV 62
           +SPLI LSL  +V+SSS LWA AS  K+Q FLQCL +HS D+Y I+KVVY+PINSSY+SV
Sbjct: 4   TSPLILLSLVLIVVSSSPLWASASEQKYQHFLQCLLEHSLDNYPISKVVYSPINSSYSSV 63

Query: 63  LNFSIRNQRFSTAKTPKPLVIIRPSKVSHIQAAVICSKSHGLQIRIRSGGHDFEGLSYAA 122
           L+FSIRN RFS  +TPKPL+II PS VSHIQAAVICSKSHGLQIR RSGGHDFEGLSY A
Sbjct: 64  LDFSIRNLRFSKPQTPKPLLIITPSHVSHIQAAVICSKSHGLQIRTRSGGHDFEGLSYVA 123

Query: 123 YLPFVVVDLIALRAVTVDVKNSIAWVQSGATLGELYYRIAEKSRTLAFPAGVCPSVGVGG 182
           YLPF++VDLI LR+VT+DV+NS AWVQSGATLGELYYRI EKSRTLAFPAGVCP+VGVGG
Sbjct: 124 YLPFIIVDLINLRSVTIDVENSTAWVQSGATLGELYYRIGEKSRTLAFPAGVCPTVGVGG 183

Query: 183 QFSGGGYGLMLRKYGIAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVA 242
            FSGGGYG MLRK+G+AADNVIDAYLVDANGK HDRESMGEDLFWAIRGGGGGSFGIVVA
Sbjct: 184 HFSGGGYGFMLRKFGLAADNVIDAYLVDANGKVHDRESMGEDLFWAIRGGGGGSFGIVVA 243

Query: 243 WKVKLVPVPATVTICTIYRTQKEDAIKLIHRWQYVANNLDENILLGIFLTGGNISTQRGG 302
           WKVKLVPVPATVT C+ +R  + DAIKL+H+WQYVAN LDEN+ LGI LTGGNI+T + G
Sbjct: 244 WKVKLVPVPATVTSCSTHRNLEGDAIKLVHQWQYVANKLDENLFLGILLTGGNITTTQEG 303

Query: 303 KINPRALFFSLFMGKTDELLAILKTTFPELGFTKEECRETSWIESALIAANGFQIGETLE 362
             +P A FF LF+GK DEL+A L TTFPEL   K++C ETSWIES LIAA G QI E+LE
Sbjct: 304 IADPTATFFILFLGKVDELVATLSTTFPELSLMKQDCVETSWIESTLIAATGVQIVESLE 363

Query: 363 ALLNRSALIPPISRGSSKIKSDYIKEPITEAAIEGIWERSKSQNIESTQVVFIPYGGRMS 422
            LLNR+    P +  S+KIKSDYIKEPI EA IEGI++R K+Q+I ++Q++F+PYGGRMS
Sbjct: 364 PLLNRT----PPTIESTKIKSDYIKEPIPEATIEGIFQRLKAQDIATSQLIFVPYGGRMS 423

Query: 423 QISESEIPYSHRAAYLYKIGYIIGWQEQSVEAEKRHINWIRELYSYMTPFVSKSPRAAYV 482
           QISESE P+SHRAA LYKIGYI+GW+EQS+ A+KRHI+WIRE+Y YMTPFVSKSPR+AY 
Sbjct: 424 QISESETPFSHRAANLYKIGYIVGWKEQSLNAKKRHISWIREVYEYMTPFVSKSPRSAYA 483

Query: 483 NYRDLDIGSNNKNGKTSYKKAMTWGLKYFGKNNFNRLVRVKTEVDPDNFFRHEQSIPTL 542
           NYRDLDIG+NNK  KTS K+A  WGLKYFG NNF RLV VKT+VDP +FFRHEQSIP L
Sbjct: 484 NYRDLDIGANNKYRKTSVKQASVWGLKYFG-NNFKRLVYVKTKVDPYDFFRHEQSIPAL 537

BLAST of Tan0003944 vs. ExPASy TrEMBL
Match: A0A6J1DHZ2 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111020223 PE=3 SV=1)

HSP 1 Score: 829.3 bits (2141), Expect = 9.2e-237
Identity = 408/543 (75.14%), Postives = 466/543 (85.82%), Query Frame = 0

Query: 3   SSPLIPL--SLAFVVLSSSSLWAMASYDKHQDFLQCLFDHSKDS-YSITKVVYTPINSSY 62
           SSPLIPL  SL F+ LSSSSLWA AS DKH+ FL CL DHS DS YSI+KVVYTP+NSSY
Sbjct: 4   SSPLIPLSFSLLFIFLSSSSLWAAASVDKHESFLNCLLDHSPDSNYSISKVVYTPLNSSY 63

Query: 63  TSVLNFSIRNQRFSTAKTPKPLVIIRPSKVSHIQAAVICSKSHGLQIRIRSGGHDFEGLS 122
           +SVL+FSIRN RFSTA TPKPL II P  VSH+QAAV+CS++HGLQIR RSGGHDFEGLS
Sbjct: 64  SSVLDFSIRNLRFSTADTPKPLFIITPLHVSHVQAAVVCSRTHGLQIRTRSGGHDFEGLS 123

Query: 123 YAAYLPFVVVDLIALRAVTVDVKNSIAWVQSGATLGELYYRIAEKSRTLAFPAGVCPSVG 182
             ++LPF+++DLI LR++T+D  N+ AWVQSGATLGELYY IAEKSRTLAFPAG CP+VG
Sbjct: 124 CVSHLPFIIIDLINLRSITIDANNNTAWVQSGATLGELYYSIAEKSRTLAFPAGACPTVG 183

Query: 183 VGGQFSGGGYGLMLRKYGIAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGI 242
           +GG FSGGGYGLMLRK+G+AADNVIDAYLVDANGKF DRE MGEDLFWAIRGGGGGSFGI
Sbjct: 184 IGGHFSGGGYGLMLRKFGLAADNVIDAYLVDANGKFQDRELMGEDLFWAIRGGGGGSFGI 243

Query: 243 VVAWKVKLVPVPATVTICTIYRTQKEDAIKLIHRWQYVANNLDENILLGIFLTGGNISTQ 302
           VVAWKVKLVPVPATVT C   RT +E AIKLIHRWQYVAN LDEN+ LGI L GGN + Q
Sbjct: 244 VVAWKVKLVPVPATVTSCYYNRTLEEGAIKLIHRWQYVANKLDENLFLGILLMGGNFTAQ 303

Query: 303 RGGKINPRALFFSLFMGKTDELLAILKTTFPELGFTKEECRETSWIESALIAANGFQIGE 362
            G K NP A FFSLF+GK DE  AIL TTFPELG  KE C E+SWIESAL++A GF+IG+
Sbjct: 304 GGDKTNPTASFFSLFLGKADEFFAILNTTFPELGLAKENCLESSWIESALLSA-GFKIGD 363

Query: 363 TLEALLNRSALIPPISRGSSKIKSDYIKEPITEAAIEGIWERSKSQNIESTQVVFIPYGG 422
           +LEALLNR+    P++ GS KIKSDY+KEPI+EA IEGIWER KS+++ + Q++F+PYGG
Sbjct: 364 SLEALLNRT----PLTNGSIKIKSDYVKEPISEATIEGIWERLKSRDVGARQLLFVPYGG 423

Query: 423 RMSQISESEIPYSHRAAYLYKIGYIIGWQEQSVEAEKRHINWIRELYSYMTPFVSKSPRA 482
           RMSQISESEIP+SHRA  LYKIGYI+ WQ+Q+V+ EKRH+ WIRELYSYMTPFVSKSPR+
Sbjct: 424 RMSQISESEIPFSHRAGNLYKIGYIVAWQKQNVDVEKRHLKWIRELYSYMTPFVSKSPRS 483

Query: 483 AYVNYRDLDIGSNNKNGKTSYKKAMTWGLKYFGKNNFNRLVRVKTEVDPDNFFRHEQSIP 542
           AYVNYRDLDIGSNNK GKTS+K+A  WG KYFG NNFNRLV VKT+VDP +FFRHEQSIP
Sbjct: 484 AYVNYRDLDIGSNNKYGKTSFKRASVWGWKYFG-NNFNRLVHVKTKVDPYDFFRHEQSIP 540

BLAST of Tan0003944 vs. ExPASy TrEMBL
Match: A0A6J1KJP1 (berberine bridge enzyme-like 27 OS=Cucurbita maxima OX=3661 GN=LOC111495223 PE=3 SV=1)

HSP 1 Score: 819.7 bits (2116), Expect = 7.3e-234
Identity = 400/542 (73.80%), Postives = 459/542 (84.69%), Query Frame = 0

Query: 1   MKSSPLIPLSLAFVVLSSSSLWAMASYDKHQDFLQCLFDHSKDSYSITKVVYTPINSSYT 60
           MKSSPL  LSL F+VL SSSLWA  S ++++DFLQCL  HS D++SITK+VYTPIN+SY+
Sbjct: 1   MKSSPLTLLSLTFIVLCSSSLWAAPSSNQNKDFLQCLLHHSPDTHSITKLVYTPINASYS 60

Query: 61  SVLNFSIRNQRFSTAKTPKPLVIIRPSKVSHIQAAVICSKSHGLQIRIRSGGHDFEGLSY 120
           S+LNFSI+N RFST KTPKPL II PS + HIQAA+ICSKSHGLQIRIRSGGHD EGLSY
Sbjct: 61  SLLNFSIKNLRFSTPKTPKPLAIITPSNIHHIQAAIICSKSHGLQIRIRSGGHDLEGLSY 120

Query: 121 AAYLPFVVVDLIALRAVTVDVKNSIAWVQSGATLGELYYRIAEKSRTLAFPAGVCPSVGV 180
            AY+PF+VVDLI L  V VDV+ + AWVQSGA+LGELYYRIAEKSRTLAFPAG+CP+VGV
Sbjct: 121 VAYVPFIVVDLINLSLVEVDVEKNTAWVQSGASLGELYYRIAEKSRTLAFPAGLCPTVGV 180

Query: 181 GGQFSGGGYGLMLRKYGIAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIV 240
           GGQFSGGGYG+++RKYG+AADNV+DAYLVDANG+F DRE MGE+LFWAIRGGGGGSFG+V
Sbjct: 181 GGQFSGGGYGMLMRKYGLAADNVVDAYLVDANGEFFDREGMGEELFWAIRGGGGGSFGVV 240

Query: 241 VAWKVKLVPVPATVTICTIYRTQKEDAIKLIHRWQYVANNLDENILLGIFLTGGNISTQR 300
           VAWKVKLVPVPATVT+C   ++  + AI LI +WQYVA+ LDEN+ LGI L G       
Sbjct: 241 VAWKVKLVPVPATVTVCATSKSLVDGAINLIDKWQYVADKLDENLFLGIILAG------- 300

Query: 301 GGKINPRALFFSLFMGKTDELLAILKTTFPELGFTKEECRETSWIESALIAANGFQIGET 360
           GGK NP A+FFSLF+GK DEL+ IL   FPELG +KE+C ETSWIE+ALIAANGFQ GE 
Sbjct: 301 GGKTNPLAIFFSLFLGKADELVTILNNPFPELGLSKEDCIETSWIEAALIAANGFQTGEV 360

Query: 361 LEALLNRSALIPPISRGSSKIKSDYIKEPITEAAIEGIWERSKSQNIESTQVVFIPYGGR 420
           LEALLNRSALIPPI+ G SK KSDY+KEP+T  AIEG+W RSKSQ IE   +VFIPYGG+
Sbjct: 361 LEALLNRSALIPPINSGRSKTKSDYVKEPMTRTAIEGMWNRSKSQEIERASLVFIPYGGK 420

Query: 421 MSQISESEIPYSHRAAYLYKIGYIIGWQEQSVEAEKRHINWIRELYSYMTPFVSKSPRAA 480
           MS+ISESEIP+ HRA   Y+I YI+GW EQ V AEKRH+NWIRELYSYMTPFVSKSPRA 
Sbjct: 421 MSEISESEIPFPHRAGNRYEIDYIVGWDEQGVHAEKRHLNWIRELYSYMTPFVSKSPRAV 480

Query: 481 YVNYRDLDIGSNNKNGKTSYKKAMTWGLKYFGKNNFNRLVRVKTEVDPDNFFRHEQSIPT 540
           YVN RDLDIGSN+  G+TSYK+A  WGLKYFG NNF+RLV VKTEVDP +FFRHEQSIPT
Sbjct: 481 YVNCRDLDIGSNDMYGRTSYKRARVWGLKYFG-NNFDRLVHVKTEVDPYDFFRHEQSIPT 534

Query: 541 LT 543
           L+
Sbjct: 541 LS 534

BLAST of Tan0003944 vs. ExPASy TrEMBL
Match: A0A6J1CN68 (tetrahydrocannabinolic acid synthase-like OS=Momordica charantia OX=3673 GN=LOC111012666 PE=3 SV=1)

HSP 1 Score: 818.9 bits (2114), Expect = 1.2e-233
Identity = 396/540 (73.33%), Postives = 463/540 (85.74%), Query Frame = 0

Query: 4   SPLIPLSLAFVVLSSSSLWAMASYDKHQDFLQCLFDHSKD--SYSITKVVYTPINSSYTS 63
           SPLI LSLAF+ LSSSSLWA A  +KH+ FLQCL DHS +  +YSI+KV++TPINSSY+S
Sbjct: 5   SPLISLSLAFIFLSSSSLWASAFPNKHESFLQCLLDHSLEYSNYSISKVIHTPINSSYSS 64

Query: 64  VLNFSIRNQRFSTAKTPKPLVIIRPSKVSHIQAAVICSKSHGLQIRIRSGGHDFEGLSYA 123
           VL+FSIRN RFST +TPKPL+I+ PS VSHIQAAVICSKS+G QIR RSGGHDFEGLSY 
Sbjct: 65  VLDFSIRNLRFSTVETPKPLLIVTPSHVSHIQAAVICSKSYGFQIRTRSGGHDFEGLSYV 124

Query: 124 AYLPFVVVDLIALRAVTVDVKNSIAWVQSGATLGELYYRIAEKSRTLAFPAGVCPSVGVG 183
           A+LPF++VDLI LR++++D+K++ AWVQSGATLGELYY IA+KSRTLAFPAG+CP+VGVG
Sbjct: 125 AHLPFIIVDLINLRSISIDIKDNTAWVQSGATLGELYYSIAQKSRTLAFPAGICPTVGVG 184

Query: 184 GQFSGGGYGLMLRKYGIAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVV 243
           G FSGGGYGL+LRKYG+AADNVIDAYLVDA+GKFH+RESMGEDLFWAIRGGGGGSFGIVV
Sbjct: 185 GHFSGGGYGLLLRKYGLAADNVIDAYLVDASGKFHNRESMGEDLFWAIRGGGGGSFGIVV 244

Query: 244 AWKVKLVPVPATVTICTIYRTQKEDAIKLIHRWQYVANNLDENILLGIFLTGGNISTQRG 303
           AWKVKLVPVPATVT+C+  RT +E A+K+ HRWQYVAN LDEN+ LGI   GG I++Q G
Sbjct: 245 AWKVKLVPVPATVTLCSANRTLEEGAVKITHRWQYVANKLDENLFLGILWLGGKITSQEG 304

Query: 304 GKINPRALFFSLFMGKTDELLAILKTTFPELGFTKEECRETSWIESALIAANGFQIGETL 363
           GK NP A FFSLF+GK DELL+IL T FPEL   K++C ETSWIES ++   GF    TL
Sbjct: 305 GKTNPVATFFSLFLGKADELLSILNTKFPELDLAKKDCIETSWIESTVLMGVGFPTELTL 364

Query: 364 EALLNRSALIPPISRGSSKIKSDYIKEPITEAAIEGIWERSKSQNIESTQVVFIPYGGRM 423
           EALL+R+    P++  SSKIKSDY+KEP++EA ++GIW+R  SQ+IE   ++F+PYGGRM
Sbjct: 365 EALLSRT----PLTNTSSKIKSDYVKEPMSEAVVQGIWKRLDSQDIEGGNIIFVPYGGRM 424

Query: 424 SQISESEIPYSHRAAYLYKIGYIIGWQEQSVEAEKRHINWIRELYSYMTPFVSKSPRAAY 483
           SQISESE P+SHRA YLYKIGYI GW+EQ ++AEKRH+ WIRELY YMTPFVSKSPRAAY
Sbjct: 425 SQISESETPFSHRAGYLYKIGYIAGWEEQGIDAEKRHLRWIRELYDYMTPFVSKSPRAAY 484

Query: 484 VNYRDLDIGSNNKNGKTSYKKAMTWGLKYFGKNNFNRLVRVKTEVDPDNFFRHEQSIPTL 542
            NYRDLDIGSNNK GKTSYK+A  WGLKYFG NNFNRLV VKT+VDP +FFRHEQSIP L
Sbjct: 485 ANYRDLDIGSNNKFGKTSYKQASVWGLKYFG-NNFNRLVHVKTKVDPYDFFRHEQSIPAL 539

BLAST of Tan0003944 vs. ExPASy TrEMBL
Match: A0A6J1EMV2 (berberine bridge enzyme-like 27 OS=Cucurbita moschata OX=3662 GN=LOC111434009 PE=3 SV=1)

HSP 1 Score: 809.3 bits (2089), Expect = 9.8e-231
Identity = 394/542 (72.69%), Postives = 455/542 (83.95%), Query Frame = 0

Query: 1   MKSSPLIPLSLAFVVLSSSSLWAMASYDKHQDFLQCLFDHSKDSYSITKVVYTPINSSYT 60
           MKSSPL  LSL F+VL SSSLWA  S ++++DFLQCL  HS D++SITK+VYTPIN+SY+
Sbjct: 1   MKSSPLTLLSLTFIVLCSSSLWAAPSSNQNKDFLQCLLHHSPDTHSITKLVYTPINASYS 60

Query: 61  SVLNFSIRNQRFSTAKTPKPLVIIRPSKVSHIQAAVICSKSHGLQIRIRSGGHDFEGLSY 120
           S+LNFSI+N RFST KTPKPL II PS + HIQAA+ICSKSHGLQIRIRSGGHD EGLSY
Sbjct: 61  SLLNFSIKNLRFSTPKTPKPLAIITPSNIPHIQAAIICSKSHGLQIRIRSGGHDLEGLSY 120

Query: 121 AAYLPFVVVDLIALRAVTVDVKNSIAWVQSGATLGELYYRIAEKSRTLAFPAGVCPSVGV 180
            A++PF+VVDLI L +V VDV+ + AWVQSGA+LGELYYRIAEKSRTL FPAG+CP+VGV
Sbjct: 121 VAHVPFIVVDLINLSSVEVDVEKNTAWVQSGASLGELYYRIAEKSRTLTFPAGLCPTVGV 180

Query: 181 GGQFSGGGYGLMLRKYGIAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIV 240
           GGQFSGGGYG+++RKYG+AADNV+DAYLVD NG+F DRE MGE+LFWAIRGGGGGSFG+V
Sbjct: 181 GGQFSGGGYGMLMRKYGLAADNVVDAYLVDGNGEFFDREGMGEELFWAIRGGGGGSFGVV 240

Query: 241 VAWKVKLVPVPATVTICTIYRTQKEDAIKLIHRWQYVANNLDENILLGIFLTGGNISTQR 300
           VAWKVKLVPVPATVT+C   ++  + AI LI +WQYVA+ LDEN+ LGI L G       
Sbjct: 241 VAWKVKLVPVPATVTVCATSKSLVDGAISLIDKWQYVADKLDENLFLGIILAG------- 300

Query: 301 GGKINPRALFFSLFMGKTDELLAILKTTFPELGFTKEECRETSWIESALIAANGFQIGET 360
           GGK NP A+FFSLF+GK DEL+ IL   FPELG  K +C ETSWIE+ALIAANGFQ GE 
Sbjct: 301 GGKTNPLAIFFSLFLGKADELVTILNNAFPELGLPKGDCIETSWIEAALIAANGFQTGEV 360

Query: 361 LEALLNRSALIPPISRGSSKIKSDYIKEPITEAAIEGIWERSKSQNIESTQVVFIPYGGR 420
           LEALLNRSALIPPI+ G SK KSDY+KEP+T  AIEG+W RSKSQ IE   +VFIPYGG+
Sbjct: 361 LEALLNRSALIPPINSGKSKTKSDYVKEPMTRTAIEGMWNRSKSQEIERASLVFIPYGGK 420

Query: 421 MSQISESEIPYSHRAAYLYKIGYIIGWQEQSVEAEKRHINWIRELYSYMTPFVSKSPRAA 480
           MS+ISESEIP+ HRA   Y+I YI+GW EQ V AEKRH+NWIRELYSYM PFVSKSPRAA
Sbjct: 421 MSEISESEIPFPHRAGNRYEIDYIVGWDEQGVHAEKRHLNWIRELYSYMAPFVSKSPRAA 480

Query: 481 YVNYRDLDIGSNNKNGKTSYKKAMTWGLKYFGKNNFNRLVRVKTEVDPDNFFRHEQSIPT 540
           YVN RDLDIGSN+  G+TSY++A  WGLKYFG NNF+RL  VKTEVDP +FFRHEQSIPT
Sbjct: 481 YVNCRDLDIGSNDMYGRTSYQRARVWGLKYFG-NNFDRLAHVKTEVDPYDFFRHEQSIPT 534

Query: 541 LT 543
           L+
Sbjct: 541 LS 534

BLAST of Tan0003944 vs. ExPASy TrEMBL
Match: A0A6J1CKW8 (berberine bridge enzyme-like 18 OS=Momordica charantia OX=3673 GN=LOC111012562 PE=3 SV=1)

HSP 1 Score: 805.1 bits (2078), Expect = 1.8e-229
Identity = 395/540 (73.15%), Postives = 456/540 (84.44%), Query Frame = 0

Query: 3   SSPLIPLSLAFVVLSSSSLWAMASYDKHQDFLQCLFDHSKD-SYSITKVVYTPINSSYTS 62
           SSPLIPL LAF+ LSSS  W +A  DKH+ FLQCL +HS D +YSI+KV++TPINSSY+S
Sbjct: 6   SSPLIPLFLAFIFLSSSRFWTLAVADKHESFLQCLLNHSPDGNYSISKVIHTPINSSYSS 65

Query: 63  VLNFSIRNQRFSTAKTPKPLVIIRPSKVSHIQAAVICSKSHGLQIRIRSGGHDFEGLSYA 122
           VL+FSIRN RFSTA+TPKPL+II PS VSHIQAAV+CSK +G QIR RSGGHDFE LSY 
Sbjct: 66  VLDFSIRNLRFSTAETPKPLLIITPSHVSHIQAAVVCSKRYGFQIRTRSGGHDFERLSYV 125

Query: 123 AYLPFVVVDLIALRAVTVDVKNSIAWVQSGATLGELYYRIAEKSRTLAFPAGVCPSVGVG 182
           A+L F++VDLI LR+++VDVKN+ AWV SGATLGELYY IA+KSRT AFPAGVCP+VGVG
Sbjct: 126 AHLSFIIVDLINLRSISVDVKNNNAWVHSGATLGELYYSIAQKSRTSAFPAGVCPTVGVG 185

Query: 183 GQFSGGGYGLMLRKYGIAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVV 242
           G FSGGGYGL+LRKYG+AADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVV
Sbjct: 186 GHFSGGGYGLLLRKYGLAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVV 245

Query: 243 AWKVKLVPVPATVTICTIYRTQKEDAIKLIHRWQYVANNLDENILLGIFLTGGNISTQRG 302
           AWKVKLVPVPATVT+C   RT +E A+KL HRWQYVAN LDEN+ LGI   GGN+++Q G
Sbjct: 246 AWKVKLVPVPATVTLCQTNRTLEEGAVKLTHRWQYVANKLDENLFLGILWLGGNVTSQGG 305

Query: 303 GKINPRALFFSLFMGKTDELLAILKTTFPELGFTKEECRETSWIESALIAANGFQIGETL 362
           GK NP A FFSLF+G+ DELLAIL T FPEL   K++C ETSWIES ++   GFQ   TL
Sbjct: 306 GKTNPVATFFSLFLGQADELLAILNTKFPELDLAKKDCIETSWIESTVLMGIGFQTKVTL 365

Query: 363 EALLNRSALIPPISRGSSKIKSDYIKEPITEAAIEGIWERSKSQNIESTQVVFIPYGGRM 422
           EALL+R+    P++  S+KIKSDY+KEPI+EA I+GI ER  +Q+IES  ++F+PYGGRM
Sbjct: 366 EALLSRT----PLTNISTKIKSDYVKEPISEATIQGIGERLNAQDIESGNLIFVPYGGRM 425

Query: 423 SQISESEIPYSHRAAYLYKIGYIIGWQEQSVEAEKRHINWIRELYSYMTPFVSKSPRAAY 482
           SQI ESE P+SHRA YLYKIGYI  W +QS++ EKRH++WIRELYSYM PFVSKSPRA Y
Sbjct: 426 SQIFESETPFSHRAGYLYKIGYITSWLDQSIDTEKRHLSWIRELYSYMAPFVSKSPRAVY 485

Query: 483 VNYRDLDIGSNNKNGKTSYKKAMTWGLKYFGKNNFNRLVRVKTEVDPDNFFRHEQSIPTL 542
            NYRDLDIGSN + GKTSYK+A TWG KYFG NNFNRLV VKT+VDP +FFRHEQSIPTL
Sbjct: 486 ANYRDLDIGSNKRYGKTSYKQASTWGFKYFG-NNFNRLVHVKTKVDPYDFFRHEQSIPTL 540

BLAST of Tan0003944 vs. TAIR 10
Match: AT5G44440.1 (FAD-binding Berberine family protein )

HSP 1 Score: 511.1 bits (1315), Expect = 1.1e-144
Identity = 251/515 (48.74%), Postives = 358/515 (69.51%), Query Frame = 0

Query: 30  HQDFLQCLFDHSKDSYSITKVVYTPINSSYTSVLNFSIRNQRFSTAKTPKPLVIIRPSKV 89
           H+DFL+CL     D+    KV++T  +SS+ S+L+ SI+N RFS ++TPKP+ II P K 
Sbjct: 28  HEDFLKCLSYRMNDNTVEPKVIHTSKDSSFFSILDSSIQNPRFSVSETPKPVSIITPVKA 87

Query: 90  SHIQAAVICSKSHGLQIRIRSGGHDFEGLSYAAY-LPFVVVDLIALRAVTVDVKNSIAWV 149
           S +Q  + C++ HG+ +R RS GH +EGLSY AY  PF V+DL  LR++++DV N   WV
Sbjct: 88  SDVQTVIRCAQLHGIHVRTRSAGHCYEGLSYIAYNKPFAVIDLRNLRSISLDVDNRTGWV 147

Query: 150 QSGATLGELYYRIAEKSRTLAFPAGVCPSVGVGGQFSGGGYGLMLRKYGIAADNVIDAYL 209
           Q+GAT GELYY I + +++LAFPAG+ P+VGVGGQFSGGGYG +LRKYG+AADN+IDA +
Sbjct: 148 QTGATAGELYYEIGKTTKSLAFPAGIHPTVGVGGQFSGGGYGTLLRKYGLAADNIIDALV 207

Query: 210 VDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICTIYRTQKEDAI 269
           VDA+G+  DR++MGED FWAIRGGGG SFG++++WKVKLV VP+T+T+  + +T K++A+
Sbjct: 208 VDASGRILDRQAMGEDYFWAIRGGGGSSFGVILSWKVKLVDVPSTITVFKVQKTSKKEAV 267

Query: 270 KLIHRWQYVANNLDENILLGIFLTGGNISTQRGGKINPRALFFSLFMGKTDELLAILKTT 329
           ++I +WQY A+ + +++ +         + +R  K    ALF  L++G  + LLA+++  
Sbjct: 268 RIIKKWQYAADKVPDDLFI-------RTTLERSNKNAVHALFTGLYIGPVNNLLALMEEK 327

Query: 330 FPELGFTKEECRETSWIESALIAANGFQIGETLEALLNRSALIPPISRGSSKIKSDYIKE 389
           FPELG  KE C E SWIES L  A+ F  GE+L  L NR       +  S K K D+++E
Sbjct: 328 FPELGLEKEGCEEMSWIESVLWFAD-FPKGESLGVLTNRER-----TSLSFKGKDDFVQE 387

Query: 390 PITEAAIEGIWERSKSQNIESTQVVFIPYGGRMSQISESEIPYSHRAAYLYKIGYIIGWQ 449
           PI EAAI+ IW R ++      +++  P+GG+MS+++E E P+ HR   LY+I Y+  W+
Sbjct: 388 PIPEAAIQEIWRRLEAPEARLGKIILTPFGGKMSEMAEYETPFPHRGGNLYEIQYVAYWR 447

Query: 450 EQ----SVEAEKRHINWIRELYSYMTPFVSKSPRAAYVNYRDLDIGSNNKNGKTSYKKAM 509
           E+      E +K ++ W+  +Y +MTP+VSKSPR AYVN++D+D+G      KT Y++  
Sbjct: 448 EEEDKNKTETDK-YLKWVDSVYEFMTPYVSKSPRGAYVNFKDMDLGMYLGKKKTKYEEGK 507

Query: 510 TWGLKYFGKNNFNRLVRVKTEVDPDNFFRHEQSIP 540
           +WG+KYF KNNF RLVRVKT VDP +FF  EQSIP
Sbjct: 508 SWGVKYF-KNNFERLVRVKTRVDPTDFFCDEQSIP 527

BLAST of Tan0003944 vs. TAIR 10
Match: AT2G34790.1 (FAD-binding Berberine family protein )

HSP 1 Score: 510.0 bits (1312), Expect = 2.4e-144
Identity = 260/539 (48.24%), Postives = 372/539 (69.02%), Query Frame = 0

Query: 6   LIPLSLAFVVLSSSSLWAMASYDKHQDFLQCLFDHSKDSYSITKVVYTPINSS--YTSVL 65
           L+ L L  V LSSS+L         QDF++CL D+S  S+ IT   ++P  ++  +   L
Sbjct: 14  LVTLLLISVPLSSSTL--------QQDFVKCLVDNSDVSFPITASFFSPDQNATLFKEEL 73

Query: 66  NFSIRNQRFSTAKTPKPLVIIRPSKVSHIQAAVICSKSHGLQIRIRSGGHDFEGLSYAA- 125
             + +N R+ T   PKP+ I  P   +H+QAAV+C+K   L +R+RSGGHD+EGLS+ A 
Sbjct: 74  ESTAQNLRYLTPSNPKPVFIFEPLYETHVQAAVVCAKKLQLHLRLRSGGHDYEGLSFVAE 133

Query: 126 -YLPFVVVDLIALRAVTVDVKNSIAWVQSGATLGELYYRIAEKSRTLAFPAGVCPSVGVG 185
              PFV+VDL  LR V VD+ ++ AW  +GAT+GE+YYRI EKS+T  FPAG+C S+G+G
Sbjct: 134 DETPFVIVDLSKLRQVDVDLDSNSAWAHAGATIGEVYYRIQEKSQTHGFPAGLCSSLGIG 193

Query: 186 GQFSGGGYGLMLRKYGIAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVV 245
           G   GG YG M+RK+G+ ADNV+DA +VDANG+  DR +MGED+FWAIRGGGGGSFG+++
Sbjct: 194 GHLVGGAYGSMMRKFGLGADNVLDARIVDANGQILDRAAMGEDVFWAIRGGGGGSFGVIL 253

Query: 246 AWKVKLVPVPATVTICTIYRTQKEDAIKLIHRWQYVANNLDENILLGIFLTGGNISTQRG 305
           AWK+KLVPVPATVT+ T+ +T ++D  K++++W+ +A+ LD+++ + + ++  + +T+ G
Sbjct: 254 AWKIKLVPVPATVTVFTVTKTLEQDGTKVLYKWEQIADKLDDDLFIRVIISPASKTTKPG 313

Query: 306 GKINPRALFFSLFMGKTDELLAILKTTFPELGFTKEECRETSWIESALIAANGFQIGETL 365
            +    + + + F+G ++ LL +++ +FPELG TK++C E SWI+S +  A GF      
Sbjct: 314 NRTISMS-YQAQFLGDSNRLLQVMQKSFPELGLTKKDCTEMSWIKSVMYIA-GFPNSAAP 373

Query: 366 EALLNRSALIPPISRGSSKIKSDYIKEPITEAAIEGIWERSKSQNIESTQVVFIPYGGRM 425
           EALL   +L     +   K KSD++KEPI    +EG+WER   +  +S   ++ PYGG M
Sbjct: 374 EALLAGKSLF----KNHFKAKSDFVKEPIPVEGLEGLWERFLEE--DSPLTIWNPYGGMM 433

Query: 426 SQISESEIPYSHRAAYLYKIGYIIGWQEQSVEAEKRHINWIRELYSYMTPFVSKSPRAAY 485
           S+ISESEIP+ HR   L+KI ++  WQ+  V +E+RH+ WIRE+YSYM  +VSK+PR AY
Sbjct: 434 SRISESEIPFPHRNGTLFKIQWLSTWQDGKV-SEERHMKWIREMYSYMEQYVSKNPRQAY 493

Query: 486 VNYRDLDIGSNNKNGKTSYKKAMTWGLKYFGKNNFNRLVRVKTEVDPDNFFRHEQSIPT 541
           VNYRDLD+G+N   G+T    A  WG KY+ K NF RLV++K E DPDNFFRHEQS+PT
Sbjct: 494 VNYRDLDLGTN--EGETD---AREWGAKYY-KGNFERLVKIKGEFDPDNFFRHEQSVPT 529

BLAST of Tan0003944 vs. TAIR 10
Match: AT5G44390.1 (FAD-binding Berberine family protein )

HSP 1 Score: 500.7 bits (1288), Expect = 1.4e-141
Identity = 256/540 (47.41%), Postives = 367/540 (67.96%), Query Frame = 0

Query: 9   LSLAFVVLSSSSLWAMASYDKHQDFLQCLFDHSKDSYSITKVVYTPIN--SSYTSVLNFS 68
           +S+  +  S  ++   +S     DF++CL+ ++   +++ K  +TP    S +T VL  +
Sbjct: 13  ISVFALYFSFYTITLTSSTSLQDDFIKCLYRNTNVRFTLDKTFFTPERNASIFTEVLEST 72

Query: 69  IRNQRFSTAKTPKPLVIIRPSKVSHIQAAVICSKSHGLQIRIRSGGHDFEGLSYAAYL-- 128
            +NQR+ T   PKP  I +P   SH+QA+VICSK   +  R+RSGGHD+EG+SY + +  
Sbjct: 73  AQNQRYLTKTMPKPGFIFKPVHESHVQASVICSKKLEIHFRVRSGGHDYEGVSYVSQIEK 132

Query: 129 PFVVVDLIALRAVTVDVKNSIAWVQSGATLGELYYRIAEKSRTLAFPAGVCPSVGVGGQF 188
           PFV++DL  LR + VD+K++ AWV++GAT+GELYYRIAEKS+   FPAGV PS+G+GG  
Sbjct: 133 PFVLIDLSKLRQINVDIKDTSAWVEAGATVGELYYRIAEKSKFHGFPAGVYPSLGIGGHI 192

Query: 189 SGGGYGLMLRKYGIAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWK 248
           +GG YG ++RKYG+AADNV+DA +VDANGK  DR SMGEDLFWAIRGG GGSFGI+++WK
Sbjct: 193 TGGAYGSLMRKYGLAADNVLDAKIVDANGKLLDRASMGEDLFWAIRGGSGGSFGIILSWK 252

Query: 249 VKLVPVPATVTICTIYRTQKED-AIKLIHRWQYVANNLDENILLGIFLTGGNISTQRGGK 308
           +KLVPVP T+T+ T+ +T ++D + K++ +WQ +A+NL + + L +F T   +S  +  K
Sbjct: 253 IKLVPVPETLTVFTVTKTFEQDRSFKILSKWQEIADNLVDELFLRVFFT---VSGNKANK 312

Query: 309 INPRALFFSLFMGKTDELLAILKTTFPELGFTKEECRETSWIESALIAANGFQIG--ETL 368
               A +   F+G+   L+ ++K  FPELG T+++C E SWI+S +I  +GF       +
Sbjct: 313 TVTMA-YIGQFLGEKGTLMEVMKKDFPELGLTQKDCIEMSWIDS-IIYNSGFPTNPPPPI 372

Query: 369 EALLNRSALIPPISRGSSKIKSDYIKEPITEAAIEGIWERSKSQNIESTQVVFIPYGGRM 428
           E LL   +   PI +   K KSD+ K+PI    +EG++++   +  ++  V++ PYGG+M
Sbjct: 373 EILLQAKS---PIGKVYFKGKSDFAKKPIPVLGLEGMFKKLLEE--DAALVIWTPYGGKM 432

Query: 429 SQISESEIPYSHRAAYLYKIGYIIGWQEQSVEAEKRHINWIRELYSYMTPFVSKSPRAAY 488
            +I ESEIP+ HR    + I Y   W + S +   R   WIRELY YMTP+VS +PR AY
Sbjct: 433 DKIPESEIPFPHRNGTNFMIQYYRSWSD-SEKRPNRRTKWIRELYGYMTPYVSSNPRQAY 492

Query: 489 VNYRDLDIGSNNKNGKTSYKKAMTWGLKYFGKNNFNRLVRVKTEVDPDNFFRHEQSIPTL 542
           VNYRDLD+G N  N K+++ +A  WG  YF K+NFNRLVR+K++VDPDNFFRHEQSIPTL
Sbjct: 493 VNYRDLDLGQNKDNSKSNFIEAKIWGANYF-KDNFNRLVRIKSKVDPDNFFRHEQSIPTL 540

BLAST of Tan0003944 vs. TAIR 10
Match: AT1G30700.1 (FAD-binding Berberine family protein )

HSP 1 Score: 495.0 bits (1273), Expect = 7.9e-140
Identity = 259/539 (48.05%), Postives = 356/539 (66.05%), Query Frame = 0

Query: 9   LSLAFVVLSSSSLWAMASYDKHQD-FLQCLFDHSKDSYSITKVVYTPINSSYTSVLNFSI 68
           L L FVV     +W  +S   + + F QCL  +S   + I+  ++   N SY+SVL  +I
Sbjct: 7   LVLFFVVF----IWQSSSSSANSETFTQCLTSNSDPKHPISPAIFFSGNGSYSSVLQANI 66

Query: 69  RNQRFSTAKTPKPLVIIRPSKVSHIQAAVICSKSHGLQIRIRSGGHDFEGLSYAAY--LP 128
           RN RF+T  TPKP +II  +  SH+QAA+ C K H LQ++IRSGGHD++GLSY  Y   P
Sbjct: 67  RNLRFNTTSTPKPFLIIAATHESHVQAAITCGKRHNLQMKIRSGGHDYDGLSYVTYSGKP 126

Query: 129 FVVVDLIALRAVTVDVKNSIAWVQSGATLGELYYRIAEKSRTLAFPAGVCPSVGVGGQFS 188
           F V+D+  LR+V VDV +  AWVQ+GA LGE+YY I EKS+TLA+PAG+CP+VGVGG  S
Sbjct: 127 FFVLDMFNLRSVDVDVASKTAWVQTGAILGEVYYYIWEKSKTLAYPAGICPTVGVGGHIS 186

Query: 189 GGGYGLMLRKYGIAADNVIDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKV 248
           GGGYG M+RKYG+  DN IDA +VD NGK  DR+ MGEDL+WAI GGGGGS+G+V+A+K+
Sbjct: 187 GGGYGNMMRKYGLTVDNTIDARMVDVNGKILDRKLMGEDLYWAINGGGGGSYGVVLAYKI 246

Query: 249 KLVPVPATVTICTIYRTQKEDAIKLIHRWQYVANNLDENILLG--IFLTGGNISTQRGGK 308
            LV VP  VT+  I RT +++A  +IHRWQ VA  L + + +   I +  G +S+Q+   
Sbjct: 247 NLVEVPENVTVFRISRTLEQNATDIIHRWQQVAPKLPDELFIRTVIDVVNGTVSSQK--- 306

Query: 309 INPRALFFSLFMGKTDELLAILKTTFPELGFTKEECRETSWIESALIAANGFQIGETLEA 368
              R  F ++F+G T  LL+IL   FPELG  + +C ETSWI+S L   N  Q+G +   
Sbjct: 307 -TVRTTFIAMFLGDTTTLLSILNRRFPELGLVRSDCTETSWIQSVLFWTN-IQVGSSETL 366

Query: 369 LLNRSALIPPISRGSSKIKSDYIKEPITEAAIEGIWERSKSQNIESTQVVFIPYGGRMSQ 428
           LL R+  +  + R     KSDY++EPI+   +E IW+  K   +E   + F PYGG M +
Sbjct: 367 LLQRNQPVNYLKR-----KSDYVREPISRTGLESIWK--KMIELEIPTMAFNPYGGEMGR 426

Query: 429 ISESEIPYSHRAAYLYKIGYIIGWQEQSVEAEKRHINWIRELYSYMTPFVSKSPRAAYVN 488
           IS +  P+ +RA  L+KI Y   W+++++    R++   R+LY +MTPFVSK+PR ++ N
Sbjct: 427 ISSTVTPFPYRAGNLWKIQYGANWRDETL--TDRYMELTRKLYQFMTPFVSKNPRQSFFN 486

Query: 489 YRDLDIGSNNKNGK-TSYKKAMTWGLKYFGKNNFNRLVRVKTEVDPDNFFRHEQSIPTL 542
           YRD+D+G N+ NGK +SY +   +G KYF   NF RLV++KT VD  NFFR+EQSIP L
Sbjct: 487 YRDVDLGINSHNGKISSYVEGKRYGKKYFA-GNFERLVKIKTRVDSGNFFRNEQSIPVL 526

BLAST of Tan0003944 vs. TAIR 10
Match: AT5G44410.1 (FAD-binding Berberine family protein )

HSP 1 Score: 493.8 bits (1270), Expect = 1.8e-139
Identity = 245/522 (46.93%), Postives = 358/522 (68.58%), Query Frame = 0

Query: 25  ASYDKHQDFLQCLFDHSKDSYSITKVVYTPINSSYTSVLNFSIRNQRFSTAKTPKPLVII 84
           A    H++FL+CL     +  S  ++++T  + SY S+LN SI+N RF   +TPKP+ II
Sbjct: 29  AQRTNHENFLKCLSHRINEDDS--RIIHTSKDPSYFSILNSSIQNPRFFVLETPKPVSII 88

Query: 85  RPSKVSHIQAAVICSKSHGLQIRIRSGGHDFEGLSY-AAYLPFVVVDLIALRAVTVDVKN 144
            P + + +Q+ + C++ HG+ IR RSGGHD+EGLSY A   PFVV+DL  LR++T+DV N
Sbjct: 89  TPVQATDVQSTIKCARLHGIHIRTRSGGHDYEGLSYMAKSRPFVVIDLRNLRSITLDVDN 148

Query: 145 SIAWVQSGATLGELYYRIAEKSRTLAFPAGVCPSVGVGGQFSGGGYGLMLRKYGIAADNV 204
              WVQSGAT+GELYY I + S++LAFPAG+ P+VG+GGQF GGGYG ++RKYG++ADNV
Sbjct: 149 RTGWVQSGATIGELYYEIGKLSKSLAFPAGLYPTVGIGGQFGGGGYGTLMRKYGLSADNV 208

Query: 205 IDAYLVDANGKFHDRESMGEDLFWAIRGGGGGSFGIVVAWKVKLVPVPATVTICTIYRTQ 264
           IDA++VDANG F DR+ MGED FWAIRGGGG SF +V++WK++L+ VP+ VT+  + +T 
Sbjct: 209 IDAHIVDANGSFLDRQGMGEDFFWAIRGGGGSSFSVVLSWKIRLLDVPSVVTVFKVVKTS 268

Query: 265 KEDAIKLIHRWQYVANNLDENILLGIFLTGGNISTQRGGKINPRALFFSLFMGKTDELLA 324
           +++A+ +I++WQY+A+ +  ++ +   L       Q+  ++   A F  L++G   +LLA
Sbjct: 269 EKEAVSIINKWQYIADKVPNDLFIRAML-------QKETEV--YASFPGLYLGPVSDLLA 328

Query: 325 ILKTTFPELGFTKEECRETSWIESALIAANGFQIGETLEALLNRSALIPPISRGSSKIKS 384
           ++K  FPELG     CRE SWIES L     F  GE++E L  R       +  S K K 
Sbjct: 329 LMKDKFPELGLEIGNCREMSWIESVL----WFIKGESMEILAKRKR-----TSRSFKGKD 388

Query: 385 DYIKEPITEAAIEGIWERSKSQNIESTQVVFIPYGGRMSQISESEIPYSHRAAYLYKIGY 444
           D+I+EPI + AI+ +W R ++      +++  P+GG+MS+I+++EIP+ HR   LY+I Y
Sbjct: 389 DFIEEPIPKTAIQYLWRRFEAPEARLAKIILTPFGGKMSEIADNEIPFPHREGNLYEIQY 448

Query: 445 IIGWQEQSVEAE---KRHINWIRELYSYMTPFVSKSPRAAYVNYRDLDIGSN-NKNGKTS 504
           +  W E+  + +   ++++ W+  +Y +MTP+VSKSPR AYVN+RD+D+G     N KT 
Sbjct: 449 LAYWSEEEDKNKTNTEKYLRWVESVYEFMTPYVSKSPRRAYVNFRDIDLGMYLGLNMKTK 508

Query: 505 YKKAMTWGLKYFGKNNFNRLVRVKTEVDPDNFFRHEQSIPTL 542
           Y++A  WG+KYF KNNF+RLVRVKT VDP +FF  EQSIP +
Sbjct: 509 YEEAKVWGVKYF-KNNFDRLVRVKTNVDPMDFFCDEQSIPIM 529