Tan0003813 (gene) Snake gourd v1

Overview
NameTan0003813
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
Descriptionribonuclease MC-like
LocationLG07: 43624211 .. 43625727 (-)
RNA-Seq ExpressionTan0003813
SyntenyTan0003813
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGACGACTGAAATTGTGTTGGTTTTGAGTATGTTGATATTAATAGTGAGTTGCGAGGGGTTTAATGAGTATGACTACTTGCAGGTTGTAATTCAATGGCAACCAGCAACATGTAGTGATTTGAAGAAGTCTATGTGCTACCAAAATCCAAGTAATAGATTCAGTGTCCATGGTCTATGGCCTAGCCTGTACAACGGAAATCCGACACAATGTTATGGTAGTCCCTTCGATGTGAATGAGGTAAATATATGAATTTTAAAGTCAATTCTTTTTGTTATACAACATGGTAACATGTTGAGTATTATTCAATAGAATTATACATCGAAAGTGTTTAAGGATTCAATAGATTGTCTTATAATTTTTGTTTTTAAAGAGATTTTCTTCTGTTGGGTTGATTAAGTTTTTTTAGTTTTATTTTTTTTTAAATGTTTTTGGCATTAATGATAGAATGACCGTTATAAAAACATGTAAAAATTAAAACAAAAAAAAAATCGCATTGGATTTGTCTTATATTTGCATGTGCTCCCAGAAAAAAATATTCCCAATAATTCATAAACCTCTTCTCCACATCCCACAACATTACAACATACTATATTGTTTTATCAATGTATAAAACATTCCTGGTAAGAAGTAGTCTAACTAGGCCACCAACAAGAGCATAACTTAACTGACATCAGGTATAATTTATGGCTAAAAGGTCATGGGTTTGAAACCCCCAACCTCAATTGTTGATGTACTAAAAAAAATAGTCTAATTATGTTTCTTATCTACTTTTTAGATGTGTTTTTAAATGCCAAGCAACATCGTAGCGGATATAGAAATCATTGTAGGAAAATGGTTATCTAACATTATCTAAAAAAGTAGTTTTCAAACAACTTGTTTTTTTTTTTCGAATTTGGGTAAGAACTCAAAAGTTTCCTTAAAAAAGATGCAAGCAATGGTAGGAAAGATGAAAAAAATTGTAGTAAAATTATAAAAAACCGAGCATAAAATTTTGAAAAGCCAAAAATAAAAATTCAAAACTTGAATACTTATCAAAGGAGATCTTATTGATGACAATTGTTCAGGTGGCTACACTACAACCCGGATTGGGTGAGATCTGGCCAAATGTTGTGACCGGGGACGATGTATGGCTATGGGGACACGAGTGGGACAAGCATGGAACTTGCACTGAAACCATCTTATTTGGCGAAGAAGGATATTTCAGTTTCGCCATGCACACATACATTGATAACAATCTACTATTTATTCTAAGCAAGAACCAAATTACACCTAATGGACAAACATACACAAAGGATCAAATCTACGCAGCAATTAGAGCAAAAACTGGAAAGACACCAGCCGTTCGATGCAACTACAATCGTTGGACTGGATTACAACAATTGCATGAAATATCAATTTGTTACAATTGGGATGCTTCGGCTTTTGAAGACTGCCCCCTCATAGCCGATACTTGCTCGAGTTCTTTTGTGTGGTCTTCGTGGAGCACTGTGAAGTTTGTATCAAGTGAATAG

mRNA sequence

ATGGCGACGACTGAAATTGTGTTGGTTTTGAGTATGTTGATATTAATAGTGAGTTGCGAGGGGTTTAATGAGTATGACTACTTGCAGGTTGTAATTCAATGGCAACCAGCAACATGTAGTGATTTGAAGAAGTCTATGTGCTACCAAAATCCAAGTAATAGATTCAGTGTCCATGGTCTATGGCCTAGCCTGTACAACGGAAATCCGACACAATGTTATGGTAGTCCCTTCGATGTGAATGAGGTGGCTACACTACAACCCGGATTGGGTGAGATCTGGCCAAATGTTGTGACCGGGGACGATGTATGGCTATGGGGACACGAGTGGGACAAGCATGGAACTTGCACTGAAACCATCTTATTTGGCGAAGAAGGATATTTCAGTTTCGCCATGCACACATACATTGATAACAATCTACTATTTATTCTAAGCAAGAACCAAATTACACCTAATGGACAAACATACACAAAGGATCAAATCTACGCAGCAATTAGAGCAAAAACTGGAAAGACACCAGCCGTTCGATGCAACTACAATCGTTGGACTGGATTACAACAATTGCATGAAATATCAATTTGTTACAATTGGGATGCTTCGGCTTTTGAAGACTGCCCCCTCATAGCCGATACTTGCTCGAGTTCTTTTGTGTGGTCTTCGTGGAGCACTGTGAAGTTTGTATCAAGTGAATAG

Coding sequence (CDS)

ATGGCGACGACTGAAATTGTGTTGGTTTTGAGTATGTTGATATTAATAGTGAGTTGCGAGGGGTTTAATGAGTATGACTACTTGCAGGTTGTAATTCAATGGCAACCAGCAACATGTAGTGATTTGAAGAAGTCTATGTGCTACCAAAATCCAAGTAATAGATTCAGTGTCCATGGTCTATGGCCTAGCCTGTACAACGGAAATCCGACACAATGTTATGGTAGTCCCTTCGATGTGAATGAGGTGGCTACACTACAACCCGGATTGGGTGAGATCTGGCCAAATGTTGTGACCGGGGACGATGTATGGCTATGGGGACACGAGTGGGACAAGCATGGAACTTGCACTGAAACCATCTTATTTGGCGAAGAAGGATATTTCAGTTTCGCCATGCACACATACATTGATAACAATCTACTATTTATTCTAAGCAAGAACCAAATTACACCTAATGGACAAACATACACAAAGGATCAAATCTACGCAGCAATTAGAGCAAAAACTGGAAAGACACCAGCCGTTCGATGCAACTACAATCGTTGGACTGGATTACAACAATTGCATGAAATATCAATTTGTTACAATTGGGATGCTTCGGCTTTTGAAGACTGCCCCCTCATAGCCGATACTTGCTCGAGTTCTTTTGTGTGGTCTTCGTGGAGCACTGTGAAGTTTGTATCAAGTGAATAG

Protein sequence

MATTEIVLVLSMLILIVSCEGFNEYDYLQVVIQWQPATCSDLKKSMCYQNPSNRFSVHGLWPSLYNGNPTQCYGSPFDVNEVATLQPGLGEIWPNVVTGDDVWLWGHEWDKHGTCTETILFGEEGYFSFAMHTYIDNNLLFILSKNQITPNGQTYTKDQIYAAIRAKTGKTPAVRCNYNRWTGLQQLHEISICYNWDASAFEDCPLIADTCSSSFVWSSWSTVKFVSSE
Homology
BLAST of Tan0003813 vs. ExPASy Swiss-Prot
Match: P23540 (Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 3.8e-32
Identity = 68/194 (35.05%), Postives = 105/194 (54.12%), Query Frame = 0

Query: 25  YDYLQVVIQWQPATCSDLKKSMCYQNPSNRFSVHGLWPSLYNGNPTQC-YGSPFDVNEVA 84
           +D    V QW PA CS  K   C  +    F++HGLWP     + T C  GSPFD+ +++
Sbjct: 1   FDSFWFVQQWPPAVCSFQKSGSCPGSGLRTFTIHGLWPQGSGTSLTNCPQGSPFDITKIS 60

Query: 85  TLQPGLGEIWPNVVTGDDVWLWGHEWDKHGTCTETILFGEEGYFSFAMHTYIDNNLLFIL 144
            LQ  L  +WPNV+  ++   W HEW KHGTC+E+  F +  YF  A+    + +++  L
Sbjct: 61  HLQSQLNTLWPNVLRANNQQFWSHEWTKHGTCSES-TFNQAAYFKLAVDMRNNYDIIGAL 120

Query: 145 SKNQITPNGQTYTKDQIYAAIRAKTGKTPAVRCNYNRWTGLQQLHEISICYNWDASAFED 204
             +   PNG+T ++  I   ++AK GK P +RC  +  T +  L ++  C+  D S   D
Sbjct: 121 RPHAAGPNGRTKSRQAIKGFLKAKFGKFPGLRCRTDPQTKVSYLVQVVACFAQDGSTLID 180

Query: 205 CPLIADTCSSSFVW 218
           C    DTC ++F++
Sbjct: 181 C--TRDTCGANFIF 191

BLAST of Tan0003813 vs. ExPASy Swiss-Prot
Match: P80022 (Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2)

HSP 1 Score: 129.4 bits (324), Expect = 5.2e-29
Identity = 70/217 (32.26%), Postives = 117/217 (53.92%), Query Frame = 0

Query: 1   MATTEIVLVLSMLILIVSC----EGFNEYDYLQVVIQWQPATCSDLKKSMCYQ---NPSN 60
           MA+     +  +L++I  C        ++D+   V QW P +  D K+S CY     P+ 
Sbjct: 1   MASNSAFSLFLILLIITQCLSVLNAAKDFDFFYFVQQW-PGSYCDTKQSCCYPTTGKPAA 60

Query: 61  RFSVHGLWPSLYNGN-PTQC-YGSPFDVNEVATLQPGLGEIWPNVV--TGDDVWLWGHEW 120
            F +HGLWP+  +G  P+ C   SP+D ++++ L   + + WP +   +G     W HEW
Sbjct: 61  DFGIHGLWPNNNDGTYPSNCDPNSPYDQSQISDLISSMQQNWPTLACPSGSGSTFWSHEW 120

Query: 121 DKHGTCTETILFGEEGYFSFAMHTYIDNNLLFILSKNQITPNGQTYTKDQIYAAIRAKTG 180
           +KHGTC E++L  +  YF  A+      +LL IL    I P+G++Y    I  AI++  G
Sbjct: 121 EKHGTCAESVLTNQHAYFKKALDLKNQIDLLSILQGADIHPDGESYDLVNIRNAIKSAIG 180

Query: 181 KTPAVRCNYNRWTGLQQLHEISICYNWDASAFEDCPL 207
            TP ++CN ++ +G  QL+++ IC +   S+  +CP+
Sbjct: 181 YTPWIQCNVDQ-SGNSQLYQVYICVDGSGSSLIECPI 215

BLAST of Tan0003813 vs. ExPASy Swiss-Prot
Match: P42813 (Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 9.7e-28
Identity = 69/209 (33.01%), Postives = 116/209 (55.50%), Query Frame = 0

Query: 9   VLSMLILIVS----CEGFNEYDYLQVVIQWQPATCSDLKKSMCYQN---PSNRFSVHGLW 68
           ++S+L+++ S         ++D+   V QW P +  D +K  CY N   P+  F +HGLW
Sbjct: 10  LISLLVILPSVFSASSSSEDFDFFYFVQQW-PGSYCDTQKKCCYPNSGKPAADFGIHGLW 69

Query: 69  PSLYNGN-PTQCYGS-PFDVNEVATLQPGLGEIWPNVV--TGDDVWLWGHEWDKHGTCTE 128
           P+  +G  P+ C  S PFD + ++ L   + + WP +   +G     W HEW+KHGTC+E
Sbjct: 70  PNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSE 129

Query: 129 TILFGEEGYFSFAMHTYIDNNLLFILSKNQITPNGQTYTKDQIYAAIRAKTGKTPAVRCN 188
           +++   E YF  A++     NLL  L+K  I P+G++Y+ + I  +I+   G TP V CN
Sbjct: 130 SVIDQHE-YFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECN 189

Query: 189 YNRWTGLQQLHEISICYNWDASAFEDCPL 207
            +  +G  QL+++ +C +   S   +CP+
Sbjct: 190 RD-GSGNSQLYQVYLCVDRSGSGLIECPV 215

BLAST of Tan0003813 vs. ExPASy Swiss-Prot
Match: O80325 (Ribonuclease S-7 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 1.6e-25
Identity = 71/205 (34.63%), Postives = 106/205 (51.71%), Query Frame = 0

Query: 6   IVLVLSMLILIVSCEGFNEYDYLQVVIQWQPATCSDLKKSMCYQNPSNRFSVHGLWPSLY 65
           + +V S+++LI+S      YDY Q   Q+QPA C+  K + C   P   F+VHGLWPS  
Sbjct: 10  VTMVFSLIVLILSSSTVG-YDYFQFTQQYQPAVCNS-KPTPCKDPPDKLFTVHGLWPSNL 69

Query: 66  NG-NPTQCYGSPFDVNEVATLQPGLGEIWPNVV-TGDDVWLWGHEWDKHGTCTETILFGE 125
           NG +P  C  +  + + +  +Q  L  IWPNV+   + V  W  +W KHG+C    +  +
Sbjct: 70  NGPHPENCTNATVNPHRIKNIQAQLKIIWPNVLDRTNHVGFWNKQWIKHGSCGYPAIMND 129

Query: 126 EGYFSFAMHTYI--DNNLLFILSKNQITPNGQTYTKDQIYAAIRAKT-GKTPAVRCNYNR 185
             YF   ++ YI    N+  ILSK +I P G       I  AIR  T  K P  +C  N 
Sbjct: 130 THYFQTVINMYITQKQNVSEILSKAKIEPLGIQRPLVHIENAIRNSTNNKKPKFKCQKN- 189

Query: 186 WTGLQQLHEISICYNWDASAFEDCP 206
            +G+ +L E+ +C +   + F +CP
Sbjct: 190 -SGVTELVEVGLCSDGSLTQFRNCP 210

BLAST of Tan0003813 vs. ExPASy Swiss-Prot
Match: O80324 (Ribonuclease S-6 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 5.9e-25
Identity = 75/214 (35.05%), Postives = 109/214 (50.93%), Query Frame = 0

Query: 1   MATTEIVLVLSM---LILIVSCEGFNEYDYLQVVIQWQPATCSDLKKSMCYQNPSNRFSV 60
           M  T ++ ++ M   LI+++SC     Y+Y Q   Q+QPA C+    + C   P   F+V
Sbjct: 1   MGITGMIYMVPMVFSLIVLISCSSTMGYNYFQFTQQYQPAVCNS-NPTPCKDPPDKLFTV 60

Query: 61  HGLWPSLYNG-NPTQCYGSPFDVNEVATLQPGLGEIWPNVV-TGDDVWLWGHEWDKHGTC 120
           HGLWPS   G +P  C        ++  L   L  IWPNV+   D V  W  +W+KHG+C
Sbjct: 61  HGLWPSNDVGDDPIYCKNKTIKSQQIGNLTAQLIIIWPNVLDRTDHVGFWNRQWNKHGSC 120

Query: 121 TETILFGEE-GYFSFAMHTYI--DNNLLFILSKNQITPNGQTYTKDQIYAAIRAKT-GKT 180
            +     +E  YF   +  YI    N+  ILS+ +I P G+   +D I  AIR  T  K 
Sbjct: 121 GKAPTIKDEMHYFKTVIKMYITQKQNVSEILSRAKIEPEGKIRRRDDIINAIRLGTKDKK 180

Query: 181 PAVRCNYNRWTGLQQLHEISICYNWDASAFEDCP 206
           P ++C  N  T   +L EI+IC + + + F DCP
Sbjct: 181 PKLKCQKNNQT--TELVEITICSDRNLTQFIDCP 211

BLAST of Tan0003813 vs. NCBI nr
Match: AEM42996.1 (S-RNase [Siraitia grosvenorii])

HSP 1 Score: 359.8 bits (922), Expect = 1.8e-95
Identity = 161/229 (70.31%), Postives = 190/229 (82.97%), Query Frame = 0

Query: 1   MATTEIVLVLSMLILIVSCEGFNEYDYLQVVIQWQPATCSDLKK--SMCYQNPSNRFSVH 60
           MA  EIVLVLSML+LI SCEG NE+DYLQVV+QWQPATCS   K   +CYQNP NR+S+H
Sbjct: 1   MAKREIVLVLSMLVLIASCEGLNEFDYLQVVMQWQPATCSASNKPYPICYQNPDNRYSIH 60

Query: 61  GLWPSLYNGNPTQCYGSPFDVNEVATLQPGLGEIWPNVVTGDDVWLWGHEWDKHGTCTET 120
           G+WPSLY+G  TQC GSPF+ N ++ LQPG+ EIWPN++ G+++WLWGHEWDKHGTCTE 
Sbjct: 61  GVWPSLYSGAATQCSGSPFNANAISILQPGISEIWPNIINGNNLWLWGHEWDKHGTCTEE 120

Query: 121 ILFGEEGYFSFAMHTYIDNNLLFILSKNQITPNGQTYTKDQIYAAIRAKTGKTPAVRCNY 180
           ILF ++ YFSFAM TYID NLL +L  +QITPNGQ Y++DQ+YAAIRAKTGKTPAVRCNY
Sbjct: 121 ILFDQDRYFSFAMDTYIDYNLLVLLGNSQITPNGQIYSRDQVYAAIRAKTGKTPAVRCNY 180

Query: 181 NRWTGLQQLHEISICYNWDASAFEDCPLIADTCSSSFVWSSWSTVKFVS 228
           NRWTG QQ+HE+S+CYN DASA  DCPL A+ C + FVW SWST K VS
Sbjct: 181 NRWTGEQQMHEVSLCYNHDASAVVDCPLNANKCDNYFVWYSWSTQKAVS 229

BLAST of Tan0003813 vs. NCBI nr
Match: XP_038903744.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 357.5 bits (916), Expect = 8.9e-95
Identity = 159/230 (69.13%), Postives = 193/230 (83.91%), Query Frame = 0

Query: 1   MATTEIVLVLSMLILIVS-CEGFNEYDYLQVVIQWQPATCSDLKK--SMCYQNPSNRFSV 60
           M   EI L+LSML++IVS CEG N+YDYLQVVIQWQPATC+D KK    C+Q+P NRFS+
Sbjct: 1   MEKKEIGLILSMLVMIVSCCEGVNDYDYLQVVIQWQPATCTDWKKPYPYCFQDPDNRFSI 60

Query: 61  HGLWPSLYNGNPTQCYGSPFDVNEVATLQPGLGEIWPNVVTGDDVWLWGHEWDKHGTCTE 120
           HG+WPSLY+G  T C+GSPFDVN+++ LQPG+ EIWPNVVTG + WLWGHEWDKHGTCTE
Sbjct: 61  HGMWPSLYSGAQTHCFGSPFDVNQISILQPGISEIWPNVVTGKNQWLWGHEWDKHGTCTE 120

Query: 121 TILFGEEGYFSFAMHTYIDNNLLFILSKNQITPNGQTYTKDQIYAAIRAKTGKTPAVRCN 180
           +ILF +E YFSF M  YI+NNL FIL K+QITPNGQ Y++ QIYAAI AKTGKTPAVRCN
Sbjct: 121 SILFDQEEYFSFTMDKYIENNLSFILGKSQITPNGQLYSRAQIYAAISAKTGKTPAVRCN 180

Query: 181 YNRWTGLQQLHEISICYNWDASAFEDCPLIADTCSSSFVWSSWSTVKFVS 228
           YN W+G++QLHEI++CYNW+A+AFEDCPL A+ C ++F+W  WST K +S
Sbjct: 181 YNYWSGVEQLHEITLCYNWNATAFEDCPLNANKCDNTFMWYPWSTTKALS 230

BLAST of Tan0003813 vs. NCBI nr
Match: XP_022149793.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 354.0 bits (907), Expect = 9.8e-94
Identity = 156/224 (69.64%), Postives = 183/224 (81.70%), Query Frame = 0

Query: 6   IVLVLSMLILIVSCEGFNEYDYLQVVIQWQPATCSDLKKSMCYQNPSNRFSVHGLWPSLY 65
           IVLVLSML+L+  CEG NE+DYLQVVIQWQPATCSD  K  C+Q P NRFS+HG+WPSLY
Sbjct: 7   IVLVLSMLVLVAICEGLNEFDYLQVVIQWQPATCSDYNKPACFQAPGNRFSIHGVWPSLY 66

Query: 66  NGNPTQCYGSPFDVNEVATLQPGLGEIWPNVVTGDDVWLWGHEWDKHGTCTETILFGEEG 125
           +G PTQC GSPFD N+++ LQPG+ EIWPNV TGD+ WLW HEWDKHGTC+E+  + ++ 
Sbjct: 67  DGYPTQCSGSPFDRNQISGLQPGISEIWPNVATGDNFWLWQHEWDKHGTCSESTFYDQQE 126

Query: 126 YFSFAMHTYIDNNLLFILSKNQITPNGQTYTKDQIYAAIRAKTGKTPAVRCNYNRWTGLQ 185
           YF F M  YI NNL  ILS NQITPNG+TYT+DQ+YAAIRAKTGKTPAVRCNYN  TG  
Sbjct: 127 YFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVRCNYNSRTGAS 186

Query: 186 QLHEISICYNWDASAFEDCPLIADTCSSSFVWSSWSTVKFVSSE 230
           QLHEIS+C+  DAS FEDCPL A+ C +SFVW+SW+TVK +S E
Sbjct: 187 QLHEISLCFKKDASVFEDCPLNANKCGASFVWNSWNTVKAISEE 230

BLAST of Tan0003813 vs. NCBI nr
Match: XP_022158939.1 (ribonuclease MC-like [Momordica charantia])

HSP 1 Score: 221.5 bits (563), Expect = 7.6e-54
Identity = 111/231 (48.05%), Postives = 146/231 (63.20%), Query Frame = 0

Query: 1   MATTEIVLVLSMLILIVSCEGFNEYDYLQVVIQWQPATCSDLK-KSMCYQNPSNRFSVHG 60
           M   EI+LVLSM + I S EG NE+DY QVV+QWQPATCS    K++C Q P +R+SVHG
Sbjct: 1   MGKREILLVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHG 60

Query: 61  LWPSLYNGNPTQCYGS-PFDVNEVATLQPGLGEIWPNVVTGDDVWLWGHEWDKHGTCTET 120
           +WPSL +G    C G+ P+D   + T+Q  L + WPNV+TG +   W +EW KHGTC+  
Sbjct: 61  VWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSA 120

Query: 121 ILFGEEGYFSFAMHTYIDNNLLFILSKNQITPNGQTYTKDQIYAAIRAKTGKTPAVRCNY 180
            L  +  YF+F +  Y + +L  IL +  I PNGQ YTK  +  AI + TGK PA+RCN 
Sbjct: 121 YL-NQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNN 180

Query: 181 NRWTGLQQLHEISICYNWDASAFEDCPLIADTCSSSFVWSSWSTVKFVSSE 230
           N  TG  QLHEIS+C++ +     DCP    TC+  FVW+SWS    V+ E
Sbjct: 181 NYRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSASVAKE 230

BLAST of Tan0003813 vs. NCBI nr
Match: XP_038903348.1 (ribonuclease MC-like [Benincasa hispida])

HSP 1 Score: 204.1 bits (518), Expect = 1.3e-48
Identity = 101/222 (45.50%), Postives = 144/222 (64.86%), Query Frame = 0

Query: 1   MATTEIVLVLSMLILIV-SCEGFNEYDYLQVVIQWQPATCSDLK-KSMCYQNPSNRFSVH 60
           M   EI+LVLS+L+L+V S +G NE+DY QV+ QWQPA CS    KS C + P NRF++H
Sbjct: 1   MGKREIMLVLSILLLLVGSSKGLNEFDYYQVIFQWQPAACSSATLKSPCVKRPENRFTIH 60

Query: 61  GLWPSLYNGNPTQCYGS-PFDVNEVATLQPGLGEIWPNVVTGDDVWLWGHEWDKHGTCTE 120
           G+WPSL +GN T+C G+ P+D N++  ++  L + WP+VV G +  LW HEW  HGTC+ 
Sbjct: 61  GVWPSLTSGNATKCSGTPPYDGNKIYAIRSRLDQDWPSVVYGTNPTLWSHEWKTHGTCSN 120

Query: 121 TILFGEEGYFSFAMHTYIDNNLLFILSKNQITPNGQTYTKDQIYAAIRAKTGKTPAVRCN 180
             L  +  YF+F +  +   N++ IL K  I P+G  Y+ D I +AI   T K PA+RCN
Sbjct: 121 YYL-DQYNYFNFGLSVFGKYNMISILRKYGINPDGNDYSIDYIESAIYNATVKWPALRCN 180

Query: 181 YNRWTGLQQLHEISICYNWDASAFEDCPLIADTCSSSFVWSS 220
            N+ +G +QL+EI++C+     A +DCP  A TC + F W+S
Sbjct: 181 KNKSSGKKQLYEIALCFTKVNRALQDCPYNAVTCGNKFSWNS 221

BLAST of Tan0003813 vs. ExPASy TrEMBL
Match: K7NBR4 (S-RNase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)

HSP 1 Score: 359.8 bits (922), Expect = 8.7e-96
Identity = 161/229 (70.31%), Postives = 190/229 (82.97%), Query Frame = 0

Query: 1   MATTEIVLVLSMLILIVSCEGFNEYDYLQVVIQWQPATCSDLKK--SMCYQNPSNRFSVH 60
           MA  EIVLVLSML+LI SCEG NE+DYLQVV+QWQPATCS   K   +CYQNP NR+S+H
Sbjct: 1   MAKREIVLVLSMLVLIASCEGLNEFDYLQVVMQWQPATCSASNKPYPICYQNPDNRYSIH 60

Query: 61  GLWPSLYNGNPTQCYGSPFDVNEVATLQPGLGEIWPNVVTGDDVWLWGHEWDKHGTCTET 120
           G+WPSLY+G  TQC GSPF+ N ++ LQPG+ EIWPN++ G+++WLWGHEWDKHGTCTE 
Sbjct: 61  GVWPSLYSGAATQCSGSPFNANAISILQPGISEIWPNIINGNNLWLWGHEWDKHGTCTEE 120

Query: 121 ILFGEEGYFSFAMHTYIDNNLLFILSKNQITPNGQTYTKDQIYAAIRAKTGKTPAVRCNY 180
           ILF ++ YFSFAM TYID NLL +L  +QITPNGQ Y++DQ+YAAIRAKTGKTPAVRCNY
Sbjct: 121 ILFDQDRYFSFAMDTYIDYNLLVLLGNSQITPNGQIYSRDQVYAAIRAKTGKTPAVRCNY 180

Query: 181 NRWTGLQQLHEISICYNWDASAFEDCPLIADTCSSSFVWSSWSTVKFVS 228
           NRWTG QQ+HE+S+CYN DASA  DCPL A+ C + FVW SWST K VS
Sbjct: 181 NRWTGEQQMHEVSLCYNHDASAVVDCPLNANKCDNYFVWYSWSTQKAVS 229

BLAST of Tan0003813 vs. ExPASy TrEMBL
Match: A0A6J1D7Q5 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111018138 PE=3 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 4.7e-94
Identity = 156/224 (69.64%), Postives = 183/224 (81.70%), Query Frame = 0

Query: 6   IVLVLSMLILIVSCEGFNEYDYLQVVIQWQPATCSDLKKSMCYQNPSNRFSVHGLWPSLY 65
           IVLVLSML+L+  CEG NE+DYLQVVIQWQPATCSD  K  C+Q P NRFS+HG+WPSLY
Sbjct: 7   IVLVLSMLVLVAICEGLNEFDYLQVVIQWQPATCSDYNKPACFQAPGNRFSIHGVWPSLY 66

Query: 66  NGNPTQCYGSPFDVNEVATLQPGLGEIWPNVVTGDDVWLWGHEWDKHGTCTETILFGEEG 125
           +G PTQC GSPFD N+++ LQPG+ EIWPNV TGD+ WLW HEWDKHGTC+E+  + ++ 
Sbjct: 67  DGYPTQCSGSPFDRNQISGLQPGISEIWPNVATGDNFWLWQHEWDKHGTCSESTFYDQQE 126

Query: 126 YFSFAMHTYIDNNLLFILSKNQITPNGQTYTKDQIYAAIRAKTGKTPAVRCNYNRWTGLQ 185
           YF F M  YI NNL  ILS NQITPNG+TYT+DQ+YAAIRAKTGKTPAVRCNYN  TG  
Sbjct: 127 YFGFGMDAYIHNNLQVILSNNQITPNGRTYTRDQVYAAIRAKTGKTPAVRCNYNSRTGAS 186

Query: 186 QLHEISICYNWDASAFEDCPLIADTCSSSFVWSSWSTVKFVSSE 230
           QLHEIS+C+  DAS FEDCPL A+ C +SFVW+SW+TVK +S E
Sbjct: 187 QLHEISLCFKKDASVFEDCPLNANKCGASFVWNSWNTVKAISEE 230

BLAST of Tan0003813 vs. ExPASy TrEMBL
Match: A0A6J1E0X1 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1)

HSP 1 Score: 221.5 bits (563), Expect = 3.7e-54
Identity = 111/231 (48.05%), Postives = 146/231 (63.20%), Query Frame = 0

Query: 1   MATTEIVLVLSMLILIVSCEGFNEYDYLQVVIQWQPATCSDLK-KSMCYQNPSNRFSVHG 60
           M   EI+LVLSM + I S EG NE+DY QVV+QWQPATCS    K++C Q P +R+SVHG
Sbjct: 1   MGKREILLVLSMFLAISSSEGVNEFDYYQVVLQWQPATCSSATLKTVCVQPPEDRYSVHG 60

Query: 61  LWPSLYNGNPTQCYGS-PFDVNEVATLQPGLGEIWPNVVTGDDVWLWGHEWDKHGTCTET 120
           +WPSL +G    C G+ P+D   + T+Q  L + WPNV+TG +   W +EW KHGTC+  
Sbjct: 61  VWPSLSSGYLPTCSGTPPYDARMILTIQARLDQDWPNVITGQNPQFWSYEWTKHGTCSSA 120

Query: 121 ILFGEEGYFSFAMHTYIDNNLLFILSKNQITPNGQTYTKDQIYAAIRAKTGKTPAVRCNY 180
            L  +  YF+F +  Y + +L  IL +  I PNGQ YTK  +  AI + TGK PA+RCN 
Sbjct: 121 YL-NQFHYFNFGLWVYTNYDLQSILYQYGIYPNGQQYTKTVVQNAIYSATGKWPALRCNN 180

Query: 181 NRWTGLQQLHEISICYNWDASAFEDCPLIADTCSSSFVWSSWSTVKFVSSE 230
           N  TG  QLHEIS+C++ +     DCP    TC+  FVW+SWS    V+ E
Sbjct: 181 NYRTGNMQLHEISLCFSKNGQILIDCPFNVVTCNEYFVWNSWSYSASVAKE 230

BLAST of Tan0003813 vs. ExPASy TrEMBL
Match: A0A6J1DQK2 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111023418 PE=3 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 1.7e-46
Identity = 99/221 (44.80%), Postives = 136/221 (61.54%), Query Frame = 0

Query: 4   TEIVLVLSMLILIVSCEGFNEYDYLQVVIQWQPATC-SDLKKSMCYQNPSNRFSVHGLWP 63
           +EIVL+LSML+LI   EG NE+DY QVV+QWQPATC S      C Q P  RFS+HG+WP
Sbjct: 5   SEIVLILSMLLLIGWSEGVNEFDYYQVVLQWQPATCSSSTLVDECVQPPDERFSIHGVWP 64

Query: 64  SLYNGNPTQCYGSPFDVNEV-ATLQPGLGEIWPNVVTGDDVWLWGHEWDKHGTCTETILF 123
           SL +G+       P+D  ++ ++++  L + WP+VV   D   W HEW  HGTC+   L 
Sbjct: 65  SLLSGDAVCSGTPPYDGTQLNSSIRARLHQDWPSVVHRVDPPFWSHEWTLHGTCSYAYL- 124

Query: 124 GEEGYFSFAMHTYIDNNLLFILSKNQITPNGQTYTKDQIYAAIRAKTGKTPAVRCNYNRW 183
            +  YF F +  Y + ++L IL+   + PNG  Y +  +   I + TGK PA+RCN N W
Sbjct: 125 DQFHYFDFGLFVYGNYDVLGILNYYNVYPNGNLYARSDVENIIYSATGKWPALRCNNNFW 184

Query: 184 TGLQQLHEISICYNWDASAFEDCPLIADTCSSSFVWSSWST 223
           +G  QLHEIS+C+     + EDCP  A  C+  FVW+SWS+
Sbjct: 185 SGDVQLHEISLCFRKQDFSLEDCPYNAVKCNDYFVWNSWSS 224

BLAST of Tan0003813 vs. ExPASy TrEMBL
Match: A0A6J1DYI5 (ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025745 PE=3 SV=1)

HSP 1 Score: 185.3 bits (469), Expect = 2.9e-43
Identity = 96/224 (42.86%), Postives = 136/224 (60.71%), Query Frame = 0

Query: 1   MATTEIVLVLSMLILIVSCEGFNEYDYLQVVIQWQPATCSDLK-KSMCYQNPSNRFSVHG 60
           MA  EI+ VL +L+ I S E  + +DY QVV QWQPATC+D+   + C QNP NRFS+HG
Sbjct: 1   MAKREIIFVLVLLLFIASSEQAS-FDYYQVVFQWQPATCNDITLPTRCVQNPDNRFSIHG 60

Query: 61  LWPSLYNGNPT-QCYGSPFDVNEVATLQPGLGEIWPNVVTGDDVWLWGHEWDKHGTCTET 120
           +WPS  +G+    C G+ FD   + +++ GL + WP+V+   +   W  EW KHGTC+E 
Sbjct: 61  VWPSKTSGSRVGPCNGTAFDPATIVSIRAGLNQNWPDVIRRKNQVFWSDEWRKHGTCSED 120

Query: 121 ILFGEEGYFSFAMHTYID--NNLLFILSKNQITPNGQTYTKDQIYAAIRAKTGKTPAVRC 180
           +L  +  YF+F ++ Y D   +++  L K +I PNG+ Y K  +  AI +  GK    RC
Sbjct: 121 LL-NQLQYFTFGLNIYHDEGKDIVEKLGKKKIIPNGKKYKKADVEKAISSVIGKRLGFRC 180

Query: 181 NYNRWTGLQQLHEISICYNWDASAFEDCPLIADTCSSSFVWSSW 221
           N ++ +   QLHEIS CY  DAS  +DCP  A  C + F WSS+
Sbjct: 181 NIDQ-SNYHQLHEISFCYAKDASTLQDCPSNATNCPTKFKWSSF 221

BLAST of Tan0003813 vs. TAIR 10
Match: AT2G02990.1 (ribonuclease 1 )

HSP 1 Score: 125.2 bits (313), Expect = 6.9e-29
Identity = 69/209 (33.01%), Postives = 116/209 (55.50%), Query Frame = 0

Query: 9   VLSMLILIVS----CEGFNEYDYLQVVIQWQPATCSDLKKSMCYQN---PSNRFSVHGLW 68
           ++S+L+++ S         ++D+   V QW P +  D +K  CY N   P+  F +HGLW
Sbjct: 10  LISLLVILPSVFSASSSSEDFDFFYFVQQW-PGSYCDTQKKCCYPNSGKPAADFGIHGLW 69

Query: 69  PSLYNGN-PTQCYGS-PFDVNEVATLQPGLGEIWPNVV--TGDDVWLWGHEWDKHGTCTE 128
           P+  +G  P+ C  S PFD + ++ L   + + WP +   +G     W HEW+KHGTC+E
Sbjct: 70  PNYKDGTYPSNCDASKPFDSSTISDLLTSMKKSWPTLACPSGSGEAFWEHEWEKHGTCSE 129

Query: 129 TILFGEEGYFSFAMHTYIDNNLLFILSKNQITPNGQTYTKDQIYAAIRAKTGKTPAVRCN 188
           +++   E YF  A++     NLL  L+K  I P+G++Y+ + I  +I+   G TP V CN
Sbjct: 130 SVIDQHE-YFQTALNLKQKTNLLGALTKAGINPDGKSYSLESIRDSIKESIGFTPWVECN 189

Query: 189 YNRWTGLQQLHEISICYNWDASAFEDCPL 207
            +  +G  QL+++ +C +   S   +CP+
Sbjct: 190 RD-GSGNSQLYQVYLCVDRSGSGLIECPV 215

BLAST of Tan0003813 vs. TAIR 10
Match: AT1G14220.1 (Ribonuclease T2 family protein )

HSP 1 Score: 118.6 bits (296), Expect = 6.5e-27
Identity = 71/206 (34.47%), Postives = 110/206 (53.40%), Query Frame = 0

Query: 24  EYDYLQVVIQWQPATCSDLKKSMCYQ---NPSNRFSVHGLWPSLYNGN--PTQC-YGSPF 83
           ++D+   V+QW  A C D  ++ CY     P+  F +HGLWP+ YNG   P+ C   S F
Sbjct: 27  DFDFFYFVLQWPGAYC-DTSRACCYPTSGKPAADFGIHGLWPN-YNGGSWPSNCDPDSQF 86

Query: 84  DVNEVATLQPGLGEIWPNV--VTGDDVWLWGHEWDKHGTCTETILFGEEGYFSFAMHTYI 143
           D ++++ L   L + WP +   + +    W HEW+KHGTC+E+++   E YF  A+    
Sbjct: 87  DRSQISDLVSSLKKNWPTLSCPSNEGFNFWEHEWEKHGTCSESVMDQHE-YFENALKLKQ 146

Query: 144 DNNLLFILSKNQITPNGQTYTKDQIYAAIRAKTGKTPAVRCNYNRWTGLQQLHEISICYN 203
             NLL IL  + I P+   Y  D+I  AI+   G TP + CN +      QLH+I IC +
Sbjct: 147 KANLLQILKNSGINPDDGFYNLDKITNAIKDGIGFTPGIECNKDPERN-AQLHQIYICVD 206

Query: 204 WDASAFEDCPLI-ADTCSSSFVWSSW 221
              + F +CP++   +C S   +S +
Sbjct: 207 TSGTEFIECPVLPRGSCPSQIQFSKF 228

BLAST of Tan0003813 vs. TAIR 10
Match: AT1G26820.1 (ribonuclease 3 )

HSP 1 Score: 108.6 bits (270), Expect = 6.7e-24
Identity = 64/191 (33.51%), Postives = 94/191 (49.21%), Query Frame = 0

Query: 24  EYDYLQVVIQWQPATCSDLKKSMCYQ---NPSNRFSVHGLWPSLYNGN-PTQCY-GSPFD 83
           ++D+   V+QW  A C D + S CY     P+  F +HGLWP+   G  P  C   S FD
Sbjct: 21  DFDFFYFVLQWPGAYC-DSRHSCCYPQTGKPAADFGIHGLWPNYKTGGWPQNCNPDSRFD 80

Query: 84  VNEVATLQPGLGEIWPNV--VTGDDVWLWGHEWDKHGTCTETILFGEEGYFSFAMHTYID 143
              V+ L   L   WP +   + D +  W HEW+KHGTC E+ L  +  YF   +     
Sbjct: 81  DLRVSDLMSDLQREWPTLSCPSNDGMKFWTHEWEKHGTCAESEL-DQHDYFEAGLKLKQK 140

Query: 144 NNLLFILSKNQITPNGQTYTKDQIYAAIRAKTGKTPAVRCNYNRWTGLQQLHEISICYNW 203
            NLL  L+   I P+ + Y    I   I+   G  P + CN +  +   QL++I +C + 
Sbjct: 141 ANLLHALTNAGIKPDDKFYEMKDIENTIKQVVGFAPGIECNKDS-SHNSQLYQIYLCVDT 200

Query: 204 DASAFEDCPLI 208
            AS F +CP++
Sbjct: 201 SASKFINCPVM 208

BLAST of Tan0003813 vs. TAIR 10
Match: AT1G14210.1 (Ribonuclease T2 family protein )

HSP 1 Score: 104.0 bits (258), Expect = 1.6e-22
Identity = 71/227 (31.28%), Postives = 116/227 (51.10%), Query Frame = 0

Query: 7   VLVLSMLIL----IVSCEGFNEYDYLQVVIQWQPATCSDLKKSMC---YQNPSNRFSVHG 66
           ++++S+LIL    + S +   ++++   V  W  A C D +K  C     N ++ F +HG
Sbjct: 4   IIIVSLLILQSLVVSSSQTEPDFNFFYWVNYWPGAIC-DSQKGCCPPTKGNTASDFIIHG 63

Query: 67  LWPSLYNGN-PTQC-YGSPFDVNEVATLQPGLGEIWPN----VVTGDDVWLWGHEWDKHG 126
           LWP   NG  P  C   + FD+++++ L   + + W          ++  LW HEW+KHG
Sbjct: 64  LWPQFNNGTWPAFCDQTNLFDISKISDLVCQMEKKWTEWGVWACPSNETNLWEHEWNKHG 123

Query: 127 TCTETILFGEEGYFSFAMHTYIDNNLLFILSKNQITPNGQTYTKDQIYAAIRAKTGKTPA 186
           TC ++I F +  YF   +      +LL IL +  I PN   Y+ D+I  AI+   G  P 
Sbjct: 124 TCVQSI-FDQHSYFRTNLKFKHKVHLLNILIQKGIKPNDGFYSLDEIKNAIKCAIGFAPG 183

Query: 187 VRCNYNRWTGLQQLHEISICYNWDASAFEDCPLIAD-TCSSSFVWSS 220
           + CN +   G +QL +I IC +  A  F +CP + D +C+S   + S
Sbjct: 184 IECNED-VKGNKQLFQIYICLDNYAKEFVECPYVPDKSCASKIKFPS 227

BLAST of Tan0003813 vs. TAIR 10
Match: AT2G39780.1 (ribonuclease 2 )

HSP 1 Score: 104.0 bits (258), Expect = 1.6e-22
Identity = 58/189 (30.69%), Postives = 91/189 (48.15%), Query Frame = 0

Query: 24  EYDYLQVVIQWQPATCSDLK----KSMCYQ--NPSNRFSVHGLWPSLYNGN-PTQCYGSP 83
           E+DY  + +QW    C   +    K+ C +  +   +F++HGLWP   +G+ P+ CY S 
Sbjct: 31  EFDYFALSLQWPGTYCRGTRHCCSKNACCRGSDAPTQFTIHGLWPDYNDGSWPSCCYRSD 90

Query: 84  FDVNEVATLQPGLGEIWPNV-------VTGDDVWLWGHEWDKHGTCTETILFGEEGYFSF 143
           F   E++TL  GL + WP++         G     WGHEW+KHGTC+  +   E  YF  
Sbjct: 91  FKEKEISTLMDGLEKYWPSLSCGSPSSCNGGKGSFWGHEWEKHGTCSSPVFHDEYNYFLT 150

Query: 144 AMHTYIDNNLLFIL-SKNQITPNGQTYTKDQIYAAIRAKTGKTPAVRCNYNRWTGLQQLH 198
            ++ Y+ +N+  +L     +  N + Y    I  AI+     TP V C  +       + 
Sbjct: 151 TLNLYLKHNVTDVLYQAGYVASNSEKYPLGGIVTAIQNAFHITPEVVCKRD------AID 210

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P235403.8e-3235.05Ribonuclease MC OS=Momordica charantia OX=3673 PE=1 SV=1[more]
P800225.2e-2932.26Extracellular ribonuclease LE OS=Solanum lycopersicum OX=4081 PE=1 SV=2[more]
P428139.7e-2833.01Ribonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=RNS1 PE=1 SV=1[more]
O803251.6e-2534.63Ribonuclease S-7 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1[more]
O803245.9e-2535.05Ribonuclease S-6 OS=Pyrus pyrifolia OX=3767 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
AEM42996.11.8e-9570.31S-RNase [Siraitia grosvenorii][more]
XP_038903744.18.9e-9569.13ribonuclease MC-like [Benincasa hispida][more]
XP_022149793.19.8e-9469.64ribonuclease MC-like [Momordica charantia][more]
XP_022158939.17.6e-5448.05ribonuclease MC-like [Momordica charantia][more]
XP_038903348.11.3e-4845.50ribonuclease MC-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
K7NBR48.7e-9670.31S-RNase OS=Siraitia grosvenorii OX=190515 PE=2 SV=1[more]
A0A6J1D7Q54.7e-9469.64ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111018138 PE=3 SV=1[more]
A0A6J1E0X13.7e-5448.05ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025395 PE=3 SV=1[more]
A0A6J1DQK21.7e-4644.80ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111023418 PE=3 SV=1[more]
A0A6J1DYI52.9e-4342.86ribonuclease MC-like OS=Momordica charantia OX=3673 GN=LOC111025745 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT2G02990.16.9e-2933.01ribonuclease 1 [more]
AT1G14220.16.5e-2734.47Ribonuclease T2 family protein [more]
AT1G26820.16.7e-2433.51ribonuclease 3 [more]
AT1G14210.11.6e-2231.28Ribonuclease T2 family protein [more]
AT2G39780.11.6e-2230.69ribonuclease 2 [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001568Ribonuclease T2-likePFAMPF00445Ribonuclease_T2coord: 25..204
e-value: 4.6E-40
score: 137.7
IPR001568Ribonuclease T2-likePANTHERPTHR11240RIBONUCLEASE T2coord: 11..215
IPR036430Ribonuclease T2-like superfamilyGENE3D3.90.730.10coord: 25..215
e-value: 7.9E-52
score: 178.0
IPR036430Ribonuclease T2-like superfamilySUPERFAMILY55895Ribonuclease Rh-likecoord: 25..216
NoneNo IPR availablePANTHERPTHR11240:SF49RIBONUCLEASE 3coord: 11..215
IPR033130Ribonuclease T2, His active site 2PROSITEPS00531RNASE_T2_2coord: 104..115
IPR018188Ribonuclease T2, His active site 1PROSITEPS00530RNASE_T2_1coord: 55..62
IPR033697Ribonuclease T2, eukaryoticCDDcd01061RNase_T2_eukcoord: 25..211
e-value: 1.99878E-57
score: 178.68

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0003813.1Tan0003813.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090502 RNA phosphodiester bond hydrolysis, endonucleolytic
molecular_function GO:0033897 ribonuclease T2 activity
molecular_function GO:0003723 RNA binding