Tan0003728 (gene) Snake gourd v1

Overview


Name	Tan0003728
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	2-methylene-furan-3-one reductase-like
Location	LG11: 5435700 .. 5438294 (+)
RNA-Seq Expression	Tan0003728
Synteny	Tan0003728

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GGGTCATATGATCTGATCTGATCATGAAATTGAAAATTATTCAAAAGGGTCCAAATTGGACGCCATCGATCTCCTTGGGATTTCAAATGATCTGTGTAATAAAGTTGAAAAATCATGTAGTAGCAAGAAAATGACCAAATTCAAAGGCCTATGATTAAAATGATATTAATCCAAATTAACAATCAACCCGACCTGAAAACACAATTGAATTAGCGAGTGACTTTTTCAGATCAACTCCAAAACCTTCGCCCGCGAAGATAAATACCCAACCTCTTGGCTTCAGCAATTTCCATCTCACAGACCCACAAACGCTTTCAATTGGGATTTGTTCTTCCTTCTGCATCCATGGCTGCTCTTTCTGTTCCTACCCACATGAAGGCTTGGGTCTACCATGAATACGGCAACACTGCCGATGTTCTGAAACTCGATTCCAATTTCCCTGTTCCTCAAATCACCCAAGATCAGGTGCTCGTCAAGCTTGTTGCTGCATCTCTCAACCCCATTGATTTCAAGAGAATTCATGGAGCTTTGAAACCTTTTCACTCTTCCCCTCCGGTGAGCTATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTTTTGATTTTACTTGGGTTGCATCATTTTTCTCTGAACTTTTCTGGGTCTGTTCTTGACTTTGGATTGATGAAACCATCACTTCTGGGTATAGGTCTTTTTCATGAGTAGATATTTTGTACTTCTTTCTGGCATTTCTATCTGTGGATTAGAATTAGAGCTGATCTTGAAGAAATTCATCCAACACCTTGATGCAAGCATAGTTCACATACATAAATGAGAATATCTTGGTATATCTGGAAGGACAACATCTTTCAGATTTTAGACTGAATCTAAAAGTCAAACTACCTGCGTTTAGAAGGAAGTGGACAATATCATATCATTTGTTGAATCTTAAAGGAAAATACTGAATAAATCAGTAATACTAGTTACAGTACCTATTCTAGATCCAATATTATCAAAATCAAGGTGGCTTTTTGAGATGAGTTCGTCTGTGTGATTGCTTCTCTCATAGTTCGACTGAGAGGTTAGAGGTTTAAATCTCCCACCTCATAGGTTTGCTCGGTTGTGGGGAAGTTTGACAACTAATCTTTTTGTGTTTCTTCTTTCTTCAATTGATTTTTTCTTATTACAGTATGTACCTGGCTATGATGTTGCTGGTGTGGTGGTGAAGGTGGGAAGTGAGGTGAAAAAGTTCAAAGTAGGAGATGAAGTATATGGAGATATAAATCACAAAGCACTGGATAAGCCAAAGACTGTTGGCACTTTGGCAGAATACACTGCCACAGAAGAGAAAGTTTTGGCTCTGAAACCTAAGAATCTGAGTTTCTTAGAGGCTGCCAGCCTTCCCCTTGCCATGGAAACTGCCTATGAAGGTCTTGAGCGAGTTGGGTTCTCTGCTGGAAAATCGATTCTTGTATTGGGAGGTGCCGGGGGAGTTGGAGCCTATGTTATACAGGTAAATGATGGCTCTGCTACAATCAGTATTTGGATCAGTTGTTCTTGTTGACACTCATATCGAATTACAAAAGTTGTCTACTGAGTTTTGTATTTCTTCATTATTGGCTAATGTGATCTTGGTGTTTCTGAATGAGGCAGCTTGCAAAGCATGTGTTTGGTGCATCAAGAATAGTTGCTACTGCAAGCACATCAAAACAGGATTTCTTGAGGAGCTTGGGAGCTGATCATCCTATTGATTACACAAAGGAAAACTTTGAAGACCTTCCCGAGAAATTCGATGTAGTCTACGATGCAGTCGGTAACATCCTCATCTAATTTCTGATCTCTGACTTTCTTTAGATCTCTGCACCTGATAATAGTCAATGAGTGAAGTCTTGATTTAAAAACTGCAGGCCAGCCTGATAAAGCCGTTAAGGCGATCAAAGAAGGCGGGAATATTGTAAGCATCGCTGCACCAATTCCAGAATTTCCAGATGCTTTCTTCCTTCTCACTTCAGATGCTGCCATGTTGGAGAAACTAAATCCATATCTAGAAAGCGGGAAGGTGAAGCCGATCATCGACCCCAAATCTCCATTCCCATTCTCCAACACTCTCGAGGCATTCGCCTATCTCGAAACCTCCAGAGCTACAGGGAAAATTGTGGTTTATCCCATTCCTTAAGTTGAGAGGCATGTTTTTGGATCAGTTTTCTGTCATAATGCACTGGAGAATCCTTGTTGTGTTTGGGAATAAGTTGTGTAACATCTTCACACCATGTTTCTTGTTCTTCCTGCTTTGTTCCACTGCCTCTCCCTAATGTACATTATTGTGGTATTGCTGCTTTGGAGATGAAATGAAAATCTTTTGTCATTTCATCAATAGGCATCTTAATCTTATGTAATGAAGGAAGTGTTGATCAAATATGCACTATTTTGTTAATGTGGTGGGACAAGTATGAGTGAATGGAGCAAGCATTGGACGGCTTGCTACCTGCACTATTGAAAGTGAAAAAAAGTGAAGTAAGCTCGCTCAACACTTACAAATATTAAAATAAATCATTAAATATCTCGTACAATAACAGATGAGTCTTCTAAATTAGTGGTGTGGAGACG

mRNA sequence

GGGTCATATGATCTGATCTGATCATGAAATTGAAAATTATTCAAAAGGGTCCAAATTGGACGCCATCGATCTCCTTGGGATTTCAAATGATCTGTGTAATAAAGTTGAAAAATCATGTAGTAGCAAGAAAATGACCAAATTCAAAGGCCTATGATTAAAATGATATTAATCCAAATTAACAATCAACCCGACCTGAAAACACAATTGAATTAGCGAGTGACTTTTTCAGATCAACTCCAAAACCTTCGCCCGCGAAGATAAATACCCAACCTCTTGGCTTCAGCAATTTCCATCTCACAGACCCACAAACGCTTTCAATTGGGATTTGTTCTTCCTTCTGCATCCATGGCTGCTCTTTCTGTTCCTACCCACATGAAGGCTTGGGTCTACCATGAATACGGCAACACTGCCGATGTTCTGAAACTCGATTCCAATTTCCCTGTTCCTCAAATCACCCAAGATCAGGTGCTCGTCAAGCTTGTTGCTGCATCTCTCAACCCCATTGATTTCAAGAGAATTCATGGAGCTTTGAAACCTTTTCACTCTTCCCCTCCGTATGTACCTGGCTATGATGTTGCTGGTGTGGTGGTGAAGGTGGGAAGTGAGGTGAAAAAGTTCAAAGTAGGAGATGAAGTATATGGAGATATAAATCACAAAGCACTGGATAAGCCAAAGACTGTTGGCACTTTGGCAGAATACACTGCCACAGAAGAGAAAGTTTTGGCTCTGAAACCTAAGAATCTGAGTTTCTTAGAGGCTGCCAGCCTTCCCCTTGCCATGGAAACTGCCTATGAAGGTCTTGAGCGAGTTGGGTTCTCTGCTGGAAAATCGATTCTTGTATTGGGAGGTGCCGGGGGAGTTGGAGCCTATGTTATACAGCTTGCAAAGCATGTGTTTGGTGCATCAAGAATAGTTGCTACTGCAAGCACATCAAAACAGGATTTCTTGAGGAGCTTGGGAGCTGATCATCCTATTGATTACACAAAGGAAAACTTTGAAGACCTTCCCGAGAAATTCGATGTAGTCTACGATGCAGTCGGCCAGCCTGATAAAGCCGTTAAGGCGATCAAAGAAGGCGGGAATATTGTAAGCATCGCTGCACCAATTCCAGAATTTCCAGATGCTTTCTTCCTTCTCACTTCAGATGCTGCCATGTTGGAGAAACTAAATCCATATCTAGAAAGCGGGAAGGTGAAGCCGATCATCGACCCCAAATCTCCATTCCCATTCTCCAACACTCTCGAGGCATTCGCCTATCTCGAAACCTCCAGAGCTACAGGGAAAATTGTGGTTTATCCCATTCCTTAAGTTGAGAGGCATGTTTTTGGATCAGTTTTCTGTCATAATGCACTGGAGAATCCTTGTTGTGTTTGGGAATAAGTTGTGTAACATCTTCACACCATGTTTCTTGTTCTTCCTGCTTTGTTCCACTGCCTCTCCCTAATGTACATTATTGTGGTATTGCTGCTTTGGAGATGAAATGAAAATCTTTTGTCATTTCATCAATAGGCATCTTAATCTTATGTAATGAAGGAAGTGTTGATCAAATATGCACTATTTTGTTAATGTGGTGGGACAAGTATGAGTGAATGGAGCAAGCATTGGACGGCTTGCTACCTGCACTATTGAAAGTGAAAAAAAGTGAAGTAAGCTCGCTCAACACTTACAAATATTAAAATAAATCATTAAATATCTCGTACAATAACAGATGAGTCTTCTAAATTAGTGGTGTGGAGACG

Coding sequence (CDS)

ATGGCTGCTCTTTCTGTTCCTACCCACATGAAGGCTTGGGTCTACCATGAATACGGCAACACTGCCGATGTTCTGAAACTCGATTCCAATTTCCCTGTTCCTCAAATCACCCAAGATCAGGTGCTCGTCAAGCTTGTTGCTGCATCTCTCAACCCCATTGATTTCAAGAGAATTCATGGAGCTTTGAAACCTTTTCACTCTTCCCCTCCGTATGTACCTGGCTATGATGTTGCTGGTGTGGTGGTGAAGGTGGGAAGTGAGGTGAAAAAGTTCAAAGTAGGAGATGAAGTATATGGAGATATAAATCACAAAGCACTGGATAAGCCAAAGACTGTTGGCACTTTGGCAGAATACACTGCCACAGAAGAGAAAGTTTTGGCTCTGAAACCTAAGAATCTGAGTTTCTTAGAGGCTGCCAGCCTTCCCCTTGCCATGGAAACTGCCTATGAAGGTCTTGAGCGAGTTGGGTTCTCTGCTGGAAAATCGATTCTTGTATTGGGAGGTGCCGGGGGAGTTGGAGCCTATGTTATACAGCTTGCAAAGCATGTGTTTGGTGCATCAAGAATAGTTGCTACTGCAAGCACATCAAAACAGGATTTCTTGAGGAGCTTGGGAGCTGATCATCCTATTGATTACACAAAGGAAAACTTTGAAGACCTTCCCGAGAAATTCGATGTAGTCTACGATGCAGTCGGCCAGCCTGATAAAGCCGTTAAGGCGATCAAAGAAGGCGGGAATATTGTAAGCATCGCTGCACCAATTCCAGAATTTCCAGATGCTTTCTTCCTTCTCACTTCAGATGCTGCCATGTTGGAGAAACTAAATCCATATCTAGAAAGCGGGAAGGTGAAGCCGATCATCGACCCCAAATCTCCATTCCCATTCTCCAACACTCTCGAGGCATTCGCCTATCTCGAAACCTCCAGAGCTACAGGGAAAATTGTGGTTTATCCCATTCCTTAA

Protein sequence

MAALSVPTHMKAWVYHEYGNTADVLKLDSNFPVPQITQDQVLVKLVAASLNPIDFKRIHGALKPFHSSPPYVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKTVGTLAEYTATEEKVLALKPKNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLAKHVFGASRIVATASTSKQDFLRSLGADHPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKAIKEGGNIVSIAAPIPEFPDAFFLLTSDAAMLEKLNPYLESGKVKPIIDPKSPFPFSNTLEAFAYLETSRATGKIVVYPIP

Homology

[Close]

BLAST of Tan0003728 vs. ExPASy Swiss-Prot
Match: Q84V25 (2-methylene-furan-3-one reductase OS=Fragaria ananassa OX=3747 GN=EO PE=1 SV=1)

HSP 1 Score: 439.1 bits (1128), Expect = 4.2e-122
Identity = 221/316 (69.94%), Postives = 258/316 (81.65%), Query Frame = 0


Query: 5   SVPTHMKAWVYHEYGNTADVLKLDSNFPVPQITQDQVLVKLVAASLNPIDFKRIHGALKP 64
           S+P+  KAWVY EYG T+DVLK D +  VP++ +DQVL+K+VAASLNP+DFKR  G  K 
Sbjct: 8   SIPSVNKAWVYSEYGKTSDVLKFDPSVAVPEVKEDQVLIKVVAASLNPVDFKRALGYFKD 67

Query: 65  FHSSPPYVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKTVGTLAEYTATEEK 124
             S  P VPGYDVAGVVVKVGS+V KFKVGDEVYGD+N  AL  P   G+LAEYTA +E+
Sbjct: 68  TDSPLPTVPGYDVAGVVVKVGSQVTKFKVGDEVYGDLNEAALVNPTRFGSLAEYTAADER 127

Query: 125 VLALKPKNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLAKHVF 184
           VLA KPK+LSF+EAASLPLA+ETAYEGLER   SAGKSILVLGGAGGVG ++IQLAKHVF
Sbjct: 128 VLAHKPKDLSFIEAASLPLAIETAYEGLERAELSAGKSILVLGGAGGVGTHIIQLAKHVF 187

Query: 185 GASRIVATASTSKQDFLRSLGADHPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKAIKEG 244
           GAS++ ATAST K DFLR+LG D  IDYTKEN EDLPEKFDVVYDAVG+ DKAVKA+KEG
Sbjct: 188 GASKVAATASTKKLDFLRTLGVDLAIDYTKENIEDLPEKFDVVYDAVGETDKAVKAVKEG 247

Query: 245 GNIVSIAAPIPEFPDAFFLLTSDAAMLEKLNPYLESGKVKPIIDPKSPFPFSNTLEAFAY 304
           G +V+I  P    P   F+LTS  ++LEKL PYLESGKVKP++DP SP+PF+  +EAF Y
Sbjct: 248 GKVVTIVGPATP-PAIHFVLTSKGSVLEKLKPYLESGKVKPVLDPTSPYPFTKLVEAFGY 307

Query: 305 LETSRATGKIVVYPIP 321
           LE+SRATGK+VVYPIP
Sbjct: 308 LESSRATGKVVVYPIP 322

[Close]

BLAST of Tan0003728 vs. ExPASy Swiss-Prot
Match: Q941I0 (2-methylene-furan-3-one reductase OS=Fragaria ananassa OX=3747 GN=EO PE=1 SV=2)

HSP 1 Score: 434.9 bits (1117), Expect = 8.0e-121
Identity = 221/317 (69.72%), Postives = 258/317 (81.39%), Query Frame = 0


Query: 5   SVPTHMKAWVYHEYGNTADVLKLDSNFPVPQITQDQVLVKLVAASLNPIDFKRIHGALKP 64
           S+P+  KAWVY EYG T+DVLK D +  VP+I +DQVL+K+VAASLNP+DFKR  G  K 
Sbjct: 8   SIPSVNKAWVYSEYGKTSDVLKFDPSVAVPEIKEDQVLIKVVAASLNPVDFKRALGYFKD 67

Query: 65  FHSSPPYVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKTVGTLAEYTATEEK 124
             S  P +PGYDVAGVVVKVGS+V KFKVGDEVYGD+N  AL  P   G+LAEYTA  E+
Sbjct: 68  TDSPLPTIPGYDVAGVVVKVGSQVTKFKVGDEVYGDLNETALVNPTRFGSLAEYTAAYER 127

Query: 125 VLALKPKNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLAKHVF 184
           VLA KPKNLSF+EAASLPLA+ETA+EGLER   SAGKS+LVLGGAGGVG ++IQLAKHVF
Sbjct: 128 VLAHKPKNLSFIEAASLPLAIETAHEGLERAELSAGKSVLVLGGAGGVGTHIIQLAKHVF 187

Query: 185 GASRIVATASTSKQDFLRSLG-ADHPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKAIKE 244
           GAS++ ATAST K D LR+LG AD  IDYTKENFEDLPEKFDVVYDAVG+ DKAVKA+KE
Sbjct: 188 GASKVAATASTKKLDLLRTLGAADLAIDYTKENFEDLPEKFDVVYDAVGETDKAVKAVKE 247

Query: 245 GGNIVSIAAPIPEFPDAFFLLTSDAAMLEKLNPYLESGKVKPIIDPKSPFPFSNTLEAFA 304
           GG +V+I  P    P   F+LTS  ++LEKL PYLESGKVKP++DP SP+PF+  +EAF 
Sbjct: 248 GGKVVTIVGPATP-PAILFVLTSKGSVLEKLKPYLESGKVKPVLDPTSPYPFTKVVEAFG 307

Query: 305 YLETSRATGKIVVYPIP 321
           YLE+SRATGK+VVYPIP
Sbjct: 308 YLESSRATGKVVVYPIP 323

[Close]

BLAST of Tan0003728 vs. ExPASy Swiss-Prot
Match: O23939 (2-methylene-furan-3-one reductase OS=Fragaria vesca OX=57918 GN=EO PE=2 SV=2)

HSP 1 Score: 434.5 bits (1116), Expect = 1.0e-120
Identity = 219/316 (69.30%), Postives = 257/316 (81.33%), Query Frame = 0


Query: 5   SVPTHMKAWVYHEYGNTADVLKLDSNFPVPQITQDQVLVKLVAASLNPIDFKRIHGALKP 64
           S+P+  KAWV  EYG T+DVLK D +  VP+I +DQVL+K+VAASLNP+DFKR  G  K 
Sbjct: 8   SIPSVNKAWVXSEYGKTSDVLKFDPSVAVPEIKEDQVLIKVVAASLNPVDFKRALGYFKD 67

Query: 65  FHSSPPYVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKTVGTLAEYTATEEK 124
             S  P +PGY VAGVVVKVGS+V KFKVGDEVYGD+N  AL  P   G+LAEYTA +E+
Sbjct: 68  TDSPLPTIPGYYVAGVVVKVGSQVTKFKVGDEVYGDLNETALVNPTRFGSLAEYTAADER 127

Query: 125 VLALKPKNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLAKHVF 184
           VLA KPKNLSF+EAASLPLA+ETA+EGLER   SAGKS+LVLGGAGGVG ++IQLAKHVF
Sbjct: 128 VLAHKPKNLSFIEAASLPLAIETAHEGLERAELSAGKSVLVLGGAGGVGTHIIQLAKHVF 187

Query: 185 GASRIVATASTSKQDFLRSLGADHPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKAIKEG 244
           GAS++ ATAST K D LR+LGAD  IDYTKENFEDLPEKFDVVYDAVG+ DKAVKA+KEG
Sbjct: 188 GASKVAATASTKKLDLLRTLGADLAIDYTKENFEDLPEKFDVVYDAVGETDKAVKAVKEG 247

Query: 245 GNIVSIAAPIPEFPDAFFLLTSDAAMLEKLNPYLESGKVKPIIDPKSPFPFSNTLEAFAY 304
           G +V+I  P    P   F+LTS  ++LEKL PYLESGKVKP++DP SP+PF+  +EAF Y
Sbjct: 248 GKVVTIVGPATP-PAILFVLTSKGSVLEKLKPYLESGKVKPVLDPTSPYPFTKVVEAFGY 307

Query: 305 LETSRATGKIVVYPIP 321
           LE+SRATGK+VVYPIP
Sbjct: 308 LESSRATGKVVVYPIP 322

[Close]

BLAST of Tan0003728 vs. ExPASy Swiss-Prot
Match: K4BW79 (2-methylene-furan-3-one reductase OS=Solanum lycopersicum OX=4081 GN=EO PE=1 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 2.0e-119
Identity = 216/318 (67.92%), Postives = 262/318 (82.39%), Query Frame = 0


Query: 3   ALSVPTHMKAWVYHEYGNTADVLKLDSNFPVPQITQDQVLVKLVAASLNPIDFKRIHGAL 62
           ++S+P+ MKAW Y +YG + DVLKL+SN  VP I +DQVL+K+VAA+LNP+DFKR  G  
Sbjct: 73  SISIPSEMKAWSYTDYG-SVDVLKLESNVAVPDIKEDQVLIKIVAAALNPVDFKRRLGKF 132

Query: 63  KPFHSSPPYVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKTVGTLAEYTATE 122
           K   S  P VPGYDVAGVVVKVGS+VK  K GDEVYGDI+ KALD PK  G+LAEYTA E
Sbjct: 133 KATDSPLPTVPGYDVAGVVVKVGSQVKGLKEGDEVYGDIHEKALDGPKQFGSLAEYTAVE 192

Query: 123 EKVLALKPKNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLAKH 182
           EK++ALKPKNLSF EAA+LPLA+ETAYEGLE+ GFS+GKSILVLGGAGGVG+ VIQLAKH
Sbjct: 193 EKLVALKPKNLSFAEAAALPLAIETAYEGLEKAGFSSGKSILVLGGAGGVGSLVIQLAKH 252

Query: 183 VFGASRIVATASTSKQDFLRSLGADHPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKAIK 242
           VFGAS++ AT+ST K + L+SLGAD  IDYTKENFEDLP+KFDVVYD+VGQ +KAVK +K
Sbjct: 253 VFGASKVAATSSTGKLELLKSLGADLAIDYTKENFEDLPDKFDVVYDSVGQGEKAVKVVK 312

Query: 243 EGGNIVSIAAPIPEFPDAFFLLTSDAAMLEKLNPYLESGKVKPIIDPKSPFPFSNTLEAF 302
           EGG++V +   +   P   F++TS+  ML+KLNPYLESGKVKP+IDPK PF F   ++AF
Sbjct: 313 EGGSVVVLTGAVTP-PGFRFVVTSNGEMLKKLNPYLESGKVKPVIDPKGPFSFDKVVDAF 372

Query: 303 AYLETSRATGKIVVYPIP 321
           +YLET RATGK+V++PIP
Sbjct: 373 SYLETGRATGKVVIHPIP 388

[Close]

BLAST of Tan0003728 vs. ExPASy Swiss-Prot
Match: Q9ZUC1 (NADPH-dependent alkenal/one oxidoreductase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=AOR PE=1 SV=2)

HSP 1 Score: 407.9 bits (1047), Expect = 1.0e-112
Identity = 207/319 (64.89%), Postives = 251/319 (78.68%), Query Frame = 0


Query: 2   AALSVPTHMKAWVYHEYGNTADVLKLDSNFPVPQITQDQVLVKLVAASLNPIDFKRIHGA 61
           A  S+P  MKAWVY +YG   DVLKL+SN  VP+I +DQVL+K+VAA+LNP+D KR  G 
Sbjct: 70  ADASIPKEMKAWVYSDYGG-VDVLKLESNIVVPEIKEDQVLIKVVAAALNPVDAKRRQGK 129

Query: 62  LKPFHSSPPYVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKTVGTLAEYTAT 121
            K   S  P VPGYDVAGVVVKVGS VK  K GDEVY +++ KAL+ PK  G+LAEYTA 
Sbjct: 130 FKATDSPLPTVPGYDVAGVVVKVGSAVKDLKEGDEVYANVSEKALEGPKQFGSLAEYTAV 189

Query: 122 EEKVLALKPKNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLAK 181
           EEK+LALKPKN+ F +AA LPLA+ETA EGL R  FSAGKSILVL GAGGVG+ VIQLAK
Sbjct: 190 EEKLLALKPKNIDFAQAAGLPLAIETADEGLVRTEFSAGKSILVLNGAGGVGSLVIQLAK 249

Query: 182 HVFGASRIVATASTSKQDFLRSLGADHPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKAI 241
           HV+GAS++ ATAST K + +RSLGAD  IDYTKEN EDLP+K+DVV+DA+G  DKAVK I
Sbjct: 250 HVYGASKVAATASTEKLELVRSLGADLAIDYTKENIEDLPDKYDVVFDAIGMCDKAVKVI 309

Query: 242 KEGGNIVSIAAPIPEFPDAFFLLTSDAAMLEKLNPYLESGKVKPIIDPKSPFPFSNTLEA 301
           KEGG +V++   +   P   F++TS+  +L+KLNPY+ESGKVKP++DPK PFPFS   +A
Sbjct: 310 KEGGKVVALTGAVTP-PGFRFVVTSNGDVLKKLNPYIESGKVKPVVDPKGPFPFSRVADA 369

Query: 302 FAYLETSRATGKIVVYPIP 321
           F+YLET+ ATGK+VVYPIP
Sbjct: 370 FSYLETNHATGKVVVYPIP 386

[Close]

BLAST of Tan0003728 vs. NCBI nr
Match: XP_022961926.1 (2-methylene-furan-3-one reductase-like [Cucurbita moschata] >KAG6602443.1 2-methylene-furan-3-one reductase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7033125.1 2-methylene-furan-3-one reductase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 581.6 bits (1498), Expect = 4.0e-162
Identity = 293/320 (91.56%), Postives = 306/320 (95.62%), Query Frame = 0


Query: 1   MAALSVPTHMKAWVYHEYGNTADVLKLDSNFPVPQITQDQVLVKLVAASLNPIDFKRIHG 60
           MAA SVPTHMKAWVYH++GNTADVLKLDSNFPVPQI Q QVLVK+VAASLNPID+KRIHG
Sbjct: 1   MAAPSVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHG 60

Query: 61  ALKPFHSSPPYVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKTVGTLAEYTA 120
            LKP + SPP VPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPK VGTLAEYTA
Sbjct: 61  MLKPSNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEEKVLALKPKNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180
           TEEKVLALKP NLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA
Sbjct: 121 TEEKVLALKPNNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180

Query: 181 KHVFGASRIVATASTSKQDFLRSLGADHPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240
           KHVFGASR+VATASTSKQ+FL  LGAD PIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA
Sbjct: 181 KHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240

Query: 241 IKEGGNIVSIAAPIPEFPDAFFLLTSDAAMLEKLNPYLESGKVKPIIDPKSPFPFSNTLE 300
           IKEGGNIVSIAAP+ EFPDAFFLLTSDAAMLEKL PYLE+GKVKPIIDPKSPFPFS+TL+
Sbjct: 241 IKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTLD 300

Query: 301 AFAYLETSRATGKIVVYPIP 321
           AFAYLETSRATGK++VYPIP
Sbjct: 301 AFAYLETSRATGKVMVYPIP 320

[Close]

BLAST of Tan0003728 vs. NCBI nr
Match: XP_022990587.1 (2-methylene-furan-3-one reductase-like [Cucurbita maxima])

HSP 1 Score: 578.9 bits (1491), Expect = 2.6e-161
Identity = 292/320 (91.25%), Postives = 305/320 (95.31%), Query Frame = 0


Query: 1   MAALSVPTHMKAWVYHEYGNTADVLKLDSNFPVPQITQDQVLVKLVAASLNPIDFKRIHG 60
           MA  SVPTHMKAWVYH++GNTADVLKLDSNFPVPQI Q QVLVK+VAASLNPID+KRIHG
Sbjct: 1   MAVPSVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHG 60

Query: 61  ALKPFHSSPPYVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKTVGTLAEYTA 120
            LK  + SPP VPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPK VGTLAEYTA
Sbjct: 61  ILKASNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEEKVLALKPKNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180
           TEEKVLALKPKNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA
Sbjct: 121 TEEKVLALKPKNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180

Query: 181 KHVFGASRIVATASTSKQDFLRSLGADHPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240
           KHVFGASR+VATASTSKQ+FL  LGAD PIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA
Sbjct: 181 KHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240

Query: 241 IKEGGNIVSIAAPIPEFPDAFFLLTSDAAMLEKLNPYLESGKVKPIIDPKSPFPFSNTLE 300
           IKEGGNIVSIAAP+ EFPDAFFLLTSDAAMLEKL PYLE+GKVKPIIDPKSPFPFS+TL+
Sbjct: 241 IKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTLD 300

Query: 301 AFAYLETSRATGKIVVYPIP 321
           AFAYLETSRATGK++VYPIP
Sbjct: 301 AFAYLETSRATGKVMVYPIP 320

[Close]

BLAST of Tan0003728 vs. NCBI nr
Match: XP_038890251.1 (2-methylene-furan-3-one reductase-like [Benincasa hispida])

HSP 1 Score: 577.8 bits (1488), Expect = 5.8e-161
Identity = 286/320 (89.38%), Postives = 306/320 (95.62%), Query Frame = 0


Query: 1   MAALSVPTHMKAWVYHEYGNTADVLKLDSNFPVPQITQDQVLVKLVAASLNPIDFKRIHG 60
           MAALSVPTHMKAWVYHE+GNTADVLKLDSN+PVP+I ++Q+L+K+VAASLNPID+KRIHG
Sbjct: 1   MAALSVPTHMKAWVYHEFGNTADVLKLDSNYPVPRINENQLLIKVVAASLNPIDYKRIHG 60

Query: 61  ALKPFHSSPPYVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKTVGTLAEYTA 120
           ALK F  SPPYVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPK VGTLAEYTA
Sbjct: 61  ALKAFDYSPPYVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEEKVLALKPKNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180
           TEE+VLALKPKNLSFLEAASLPLAMETAYEGLER G SAGKSI+VLGGAGGVGAYVIQLA
Sbjct: 121 TEERVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSAGKSIIVLGGAGGVGAYVIQLA 180

Query: 181 KHVFGASRIVATASTSKQDFLRSLGADHPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240
           KHVFGAS++VATASTSKQD LRSLGAD PIDYTKENFEDL EKFD+VYDAVGQPDKAVKA
Sbjct: 181 KHVFGASKVVATASTSKQDLLRSLGADQPIDYTKENFEDLDEKFDIVYDAVGQPDKAVKA 240

Query: 241 IKEGGNIVSIAAPIPEFPDAFFLLTSDAAMLEKLNPYLESGKVKPIIDPKSPFPFSNTLE 300
           +KEGGN+VSIA P+P FPDAFFLLTSDA MLEKLNPYLESGKVKPIID KSPFPFS+TL+
Sbjct: 241 LKEGGNLVSIATPVPGFPDAFFLLTSDAVMLEKLNPYLESGKVKPIIDSKSPFPFSDTLD 300

Query: 301 AFAYLETSRATGKIVVYPIP 321
           AFAYLETSRATGKIV+YPIP
Sbjct: 301 AFAYLETSRATGKIVIYPIP 320

[Close]

BLAST of Tan0003728 vs. NCBI nr
Match: XP_023543317.1 (2-methylene-furan-3-one reductase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 569.3 bits (1466), Expect = 2.1e-158
Identity = 288/320 (90.00%), Postives = 304/320 (95.00%), Query Frame = 0


Query: 1   MAALSVPTHMKAWVYHEYGNTADVLKLDSNFPVPQITQDQVLVKLVAASLNPIDFKRIHG 60
           MAA SVPTHMKAWVYH++GNTADVLKLDS+FPVPQI Q QVLVK+VAASLNPID+KRIHG
Sbjct: 1   MAAPSVPTHMKAWVYHDFGNTADVLKLDSSFPVPQINQHQVLVKVVAASLNPIDYKRIHG 60

Query: 61  ALKPFHSSPPYVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKTVGTLAEYTA 120
           +LK  + SPP VPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPK VGTLAEYTA
Sbjct: 61  SLKASNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEEKVLALKPKNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180
           TEE VLALKP NLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA
Sbjct: 121 TEETVLALKPNNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180

Query: 181 KHVFGASRIVATASTSKQDFLRSLGADHPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240
           KHVFGASR+VATASTSKQ+FL  LGAD PIDYTKENFEDLPEKFDVVYDAVGQP+KAVKA
Sbjct: 181 KHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPNKAVKA 240

Query: 241 IKEGGNIVSIAAPIPEFPDAFFLLTSDAAMLEKLNPYLESGKVKPIIDPKSPFPFSNTLE 300
           IKEGGNIVSIAAP+ EFPDAFFLLTSDAAMLEKL PYLE+GKVKPIIDPKSPF FS+TL+
Sbjct: 241 IKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFLFSDTLD 300

Query: 301 AFAYLETSRATGKIVVYPIP 321
           AFAYLETSRATGK++VYPIP
Sbjct: 301 AFAYLETSRATGKVMVYPIP 320

[Close]

BLAST of Tan0003728 vs. NCBI nr
Match: XP_022133822.1 (2-methylene-furan-3-one reductase-like [Momordica charantia])

HSP 1 Score: 564.7 bits (1454), Expect = 5.1e-157
Identity = 279/320 (87.19%), Postives = 307/320 (95.94%), Query Frame = 0


Query: 1   MAALSVPTHMKAWVYHEYGNTADVLKLDSNFPVPQITQDQVLVKLVAASLNPIDFKRIHG 60
           MAALS+P  MKAWVYH++GNTADVLKL+S++PVPQI+++QVLV++VAASLNPIDFKRIHG
Sbjct: 1   MAALSIPNQMKAWVYHDFGNTADVLKLESSYPVPQISENQVLVRVVAASLNPIDFKRIHG 60

Query: 61  ALKPFHSSPPYVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKTVGTLAEYTA 120
            LK F+ +PP VPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPK VGTLAEYTA
Sbjct: 61  ILKAFNYNPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEEKVLALKPKNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180
           TE+KVLALKPKNLSF+EAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGA+VIQLA
Sbjct: 121 TEDKVLALKPKNLSFIEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAHVIQLA 180

Query: 181 KHVFGASRIVATASTSKQDFLRSLGADHPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240
           KHVFGA+RI ATAST+KQ+FLRSLGAD PIDYTK+NFEDLP+KFDVVYDAVGQPDKAVKA
Sbjct: 181 KHVFGAARIAATASTAKQEFLRSLGADLPIDYTKQNFEDLPDKFDVVYDAVGQPDKAVKA 240

Query: 241 IKEGGNIVSIAAPIPEFPDAFFLLTSDAAMLEKLNPYLESGKVKPIIDPKSPFPFSNTLE 300
           IKEGG +VSIAAP+P FPDAFFLLTSDA MLEKLNPYLE+GKVKPIIDPKSPFPFS+ L+
Sbjct: 241 IKEGGKLVSIAAPVPNFPDAFFLLTSDAVMLEKLNPYLENGKVKPIIDPKSPFPFSDVLQ 300

Query: 301 AFAYLETSRATGKIVVYPIP 321
           AFA+LETSRATGK+VVYPIP
Sbjct: 301 AFAHLETSRATGKVVVYPIP 320

[Close]

BLAST of Tan0003728 vs. ExPASy TrEMBL
Match: A0A6J1HBQ4 (2-methylene-furan-3-one reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111462548 PE=3 SV=1)

HSP 1 Score: 581.6 bits (1498), Expect = 2.0e-162
Identity = 293/320 (91.56%), Postives = 306/320 (95.62%), Query Frame = 0


Query: 1   MAALSVPTHMKAWVYHEYGNTADVLKLDSNFPVPQITQDQVLVKLVAASLNPIDFKRIHG 60
           MAA SVPTHMKAWVYH++GNTADVLKLDSNFPVPQI Q QVLVK+VAASLNPID+KRIHG
Sbjct: 1   MAAPSVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHG 60

Query: 61  ALKPFHSSPPYVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKTVGTLAEYTA 120
            LKP + SPP VPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPK VGTLAEYTA
Sbjct: 61  MLKPSNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEEKVLALKPKNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180
           TEEKVLALKP NLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA
Sbjct: 121 TEEKVLALKPNNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180

Query: 181 KHVFGASRIVATASTSKQDFLRSLGADHPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240
           KHVFGASR+VATASTSKQ+FL  LGAD PIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA
Sbjct: 181 KHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240

Query: 241 IKEGGNIVSIAAPIPEFPDAFFLLTSDAAMLEKLNPYLESGKVKPIIDPKSPFPFSNTLE 300
           IKEGGNIVSIAAP+ EFPDAFFLLTSDAAMLEKL PYLE+GKVKPIIDPKSPFPFS+TL+
Sbjct: 241 IKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTLD 300

Query: 301 AFAYLETSRATGKIVVYPIP 321
           AFAYLETSRATGK++VYPIP
Sbjct: 301 AFAYLETSRATGKVMVYPIP 320

[Close]

BLAST of Tan0003728 vs. ExPASy TrEMBL
Match: A0A6J1JJ69 (2-methylene-furan-3-one reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111487421 PE=3 SV=1)

HSP 1 Score: 578.9 bits (1491), Expect = 1.3e-161
Identity = 292/320 (91.25%), Postives = 305/320 (95.31%), Query Frame = 0


Query: 1   MAALSVPTHMKAWVYHEYGNTADVLKLDSNFPVPQITQDQVLVKLVAASLNPIDFKRIHG 60
           MA  SVPTHMKAWVYH++GNTADVLKLDSNFPVPQI Q QVLVK+VAASLNPID+KRIHG
Sbjct: 1   MAVPSVPTHMKAWVYHDFGNTADVLKLDSNFPVPQINQHQVLVKVVAASLNPIDYKRIHG 60

Query: 61  ALKPFHSSPPYVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKTVGTLAEYTA 120
            LK  + SPP VPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPK VGTLAEYTA
Sbjct: 61  ILKASNYSPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEEKVLALKPKNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180
           TEEKVLALKPKNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA
Sbjct: 121 TEEKVLALKPKNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180

Query: 181 KHVFGASRIVATASTSKQDFLRSLGADHPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240
           KHVFGASR+VATASTSKQ+FL  LGAD PIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA
Sbjct: 181 KHVFGASRVVATASTSKQEFLSCLGADQPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240

Query: 241 IKEGGNIVSIAAPIPEFPDAFFLLTSDAAMLEKLNPYLESGKVKPIIDPKSPFPFSNTLE 300
           IKEGGNIVSIAAP+ EFPDAFFLLTSDAAMLEKL PYLE+GKVKPIIDPKSPFPFS+TL+
Sbjct: 241 IKEGGNIVSIAAPVAEFPDAFFLLTSDAAMLEKLKPYLENGKVKPIIDPKSPFPFSDTLD 300

Query: 301 AFAYLETSRATGKIVVYPIP 321
           AFAYLETSRATGK++VYPIP
Sbjct: 301 AFAYLETSRATGKVMVYPIP 320

[Close]

BLAST of Tan0003728 vs. ExPASy TrEMBL
Match: A0A6J1BXV5 (2-methylene-furan-3-one reductase-like OS=Momordica charantia OX=3673 GN=LOC111006288 PE=3 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 2.5e-157
Identity = 279/320 (87.19%), Postives = 307/320 (95.94%), Query Frame = 0


Query: 1   MAALSVPTHMKAWVYHEYGNTADVLKLDSNFPVPQITQDQVLVKLVAASLNPIDFKRIHG 60
           MAALS+P  MKAWVYH++GNTADVLKL+S++PVPQI+++QVLV++VAASLNPIDFKRIHG
Sbjct: 1   MAALSIPNQMKAWVYHDFGNTADVLKLESSYPVPQISENQVLVRVVAASLNPIDFKRIHG 60

Query: 61  ALKPFHSSPPYVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKTVGTLAEYTA 120
            LK F+ +PP VPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPK VGTLAEYTA
Sbjct: 61  ILKAFNYNPPSVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEEKVLALKPKNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180
           TE+KVLALKPKNLSF+EAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGA+VIQLA
Sbjct: 121 TEDKVLALKPKNLSFIEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAHVIQLA 180

Query: 181 KHVFGASRIVATASTSKQDFLRSLGADHPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240
           KHVFGA+RI ATAST+KQ+FLRSLGAD PIDYTK+NFEDLP+KFDVVYDAVGQPDKAVKA
Sbjct: 181 KHVFGAARIAATASTAKQEFLRSLGADLPIDYTKQNFEDLPDKFDVVYDAVGQPDKAVKA 240

Query: 241 IKEGGNIVSIAAPIPEFPDAFFLLTSDAAMLEKLNPYLESGKVKPIIDPKSPFPFSNTLE 300
           IKEGG +VSIAAP+P FPDAFFLLTSDA MLEKLNPYLE+GKVKPIIDPKSPFPFS+ L+
Sbjct: 241 IKEGGKLVSIAAPVPNFPDAFFLLTSDAVMLEKLNPYLENGKVKPIIDPKSPFPFSDVLQ 300

Query: 301 AFAYLETSRATGKIVVYPIP 321
           AFA+LETSRATGK+VVYPIP
Sbjct: 301 AFAHLETSRATGKVVVYPIP 320

[Close]

BLAST of Tan0003728 vs. ExPASy TrEMBL
Match: A0A6J1FK96 (2-methylene-furan-3-one reductase-like OS=Cucurbita moschata OX=3662 GN=LOC111444796 PE=3 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 7.2e-157
Identity = 281/320 (87.81%), Postives = 304/320 (95.00%), Query Frame = 0


Query: 1   MAALSVPTHMKAWVYHEYGNTADVLKLDSNFPVPQITQDQVLVKLVAASLNPIDFKRIHG 60
           MAALSVPTHMKAWVYHE+GNTADVLKLD+N+PVP+I ++QVLVK+VAASLNPID+KRIHG
Sbjct: 1   MAALSVPTHMKAWVYHEFGNTADVLKLDTNYPVPEINENQVLVKVVAASLNPIDYKRIHG 60

Query: 61  ALKPFHSSPPYVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKTVGTLAEYTA 120
           +LK  +  PP +PGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPK VGTLAEYTA
Sbjct: 61  SLKALNYLPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEEKVLALKPKNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180
           TE+KVLALKPKNLSFLEAASLPLAMETAYEGLER G SAGKSI+VLGGAGGVGA+VIQLA
Sbjct: 121 TEDKVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSAGKSIIVLGGAGGVGAHVIQLA 180

Query: 181 KHVFGASRIVATASTSKQDFLRSLGADHPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240
           K+VFGAS+I ATASTSKQD LRSLGAD PIDYTKENFEDLPEKFDVVYDAVGQ DKA+KA
Sbjct: 181 KNVFGASKIAATASTSKQDLLRSLGADQPIDYTKENFEDLPEKFDVVYDAVGQADKAIKA 240

Query: 241 IKEGGNIVSIAAPIPEFPDAFFLLTSDAAMLEKLNPYLESGKVKPIIDPKSPFPFSNTLE 300
           +KEGG++VSIAAP+  FPDAFFLLTSDA +LEKLNPYLESGKVKPIIDPKSPFPFS+TLE
Sbjct: 241 MKEGGHLVSIAAPVAGFPDAFFLLTSDAVILEKLNPYLESGKVKPIIDPKSPFPFSDTLE 300

Query: 301 AFAYLETSRATGKIVVYPIP 321
           AFAYLETSRATGKIVVYPIP
Sbjct: 301 AFAYLETSRATGKIVVYPIP 320

[Close]

BLAST of Tan0003728 vs. ExPASy TrEMBL
Match: A0A6J1JTD8 (2-methylene-furan-3-one reductase-like OS=Cucurbita maxima OX=3661 GN=LOC111489564 PE=3 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 1.2e-156
Identity = 281/320 (87.81%), Postives = 304/320 (95.00%), Query Frame = 0


Query: 1   MAALSVPTHMKAWVYHEYGNTADVLKLDSNFPVPQITQDQVLVKLVAASLNPIDFKRIHG 60
           MAALSVPTHMKAWVYHE+GNTADVLKLD+N+PVP+I ++QVLVK+VAASLNPID+KRIHG
Sbjct: 1   MAALSVPTHMKAWVYHEFGNTADVLKLDTNYPVPEINENQVLVKVVAASLNPIDYKRIHG 60

Query: 61  ALKPFHSSPPYVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKTVGTLAEYTA 120
           ALK  +  PP +PGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPK VGTLAEYTA
Sbjct: 61  ALKALNYLPPSIPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKNVGTLAEYTA 120

Query: 121 TEEKVLALKPKNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLA 180
           TE+KVLALKPKNLSFLEAASLPLAMETAYEGLER G SAGKSI+VLGGAGGVGA+VIQLA
Sbjct: 121 TEDKVLALKPKNLSFLEAASLPLAMETAYEGLERAGLSAGKSIIVLGGAGGVGAHVIQLA 180

Query: 181 KHVFGASRIVATASTSKQDFLRSLGADHPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKA 240
           K+VFGAS+I ATASTSKQD LRSLGAD PIDYTKE+FEDLPEKFDVVYDAVGQ DKA+KA
Sbjct: 181 KNVFGASKIAATASTSKQDLLRSLGADQPIDYTKESFEDLPEKFDVVYDAVGQADKAIKA 240

Query: 241 IKEGGNIVSIAAPIPEFPDAFFLLTSDAAMLEKLNPYLESGKVKPIIDPKSPFPFSNTLE 300
           +KEGG++VSIAAP+  FPDAFFLLTSDA MLEKLNPYLESGKVKPIIDPKSPFPFS+TL+
Sbjct: 241 MKEGGHLVSIAAPVAGFPDAFFLLTSDAVMLEKLNPYLESGKVKPIIDPKSPFPFSDTLK 300

Query: 301 AFAYLETSRATGKIVVYPIP 321
           AFAYLETSRATGKIVVYPIP
Sbjct: 301 AFAYLETSRATGKIVVYPIP 320

[Close]

BLAST of Tan0003728 vs. TAIR 10
Match: AT1G23740.1 (Oxidoreductase, zinc-binding dehydrogenase family protein )

HSP 1 Score: 407.9 bits (1047), Expect = 7.5e-114
Identity = 207/319 (64.89%), Postives = 251/319 (78.68%), Query Frame = 0


Query: 2   AALSVPTHMKAWVYHEYGNTADVLKLDSNFPVPQITQDQVLVKLVAASLNPIDFKRIHGA 61
           A  S+P  MKAWVY +YG   DVLKL+SN  VP+I +DQVL+K+VAA+LNP+D KR  G 
Sbjct: 70  ADASIPKEMKAWVYSDYGG-VDVLKLESNIVVPEIKEDQVLIKVVAAALNPVDAKRRQGK 129

Query: 62  LKPFHSSPPYVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKTVGTLAEYTAT 121
            K   S  P VPGYDVAGVVVKVGS VK  K GDEVY +++ KAL+ PK  G+LAEYTA 
Sbjct: 130 FKATDSPLPTVPGYDVAGVVVKVGSAVKDLKEGDEVYANVSEKALEGPKQFGSLAEYTAV 189

Query: 122 EEKVLALKPKNLSFLEAASLPLAMETAYEGLERVGFSAGKSILVLGGAGGVGAYVIQLAK 181
           EEK+LALKPKN+ F +AA LPLA+ETA EGL R  FSAGKSILVL GAGGVG+ VIQLAK
Sbjct: 190 EEKLLALKPKNIDFAQAAGLPLAIETADEGLVRTEFSAGKSILVLNGAGGVGSLVIQLAK 249

Query: 182 HVFGASRIVATASTSKQDFLRSLGADHPIDYTKENFEDLPEKFDVVYDAVGQPDKAVKAI 241
           HV+GAS++ ATAST K + +RSLGAD  IDYTKEN EDLP+K+DVV+DA+G  DKAVK I
Sbjct: 250 HVYGASKVAATASTEKLELVRSLGADLAIDYTKENIEDLPDKYDVVFDAIGMCDKAVKVI 309

Query: 242 KEGGNIVSIAAPIPEFPDAFFLLTSDAAMLEKLNPYLESGKVKPIIDPKSPFPFSNTLEA 301
           KEGG +V++   +   P   F++TS+  +L+KLNPY+ESGKVKP++DPK PFPFS   +A
Sbjct: 310 KEGGKVVALTGAVTP-PGFRFVVTSNGDVLKKLNPYIESGKVKPVVDPKGPFPFSRVADA 369

Query: 302 FAYLETSRATGKIVVYPIP 321
           F+YLET+ ATGK+VVYPIP
Sbjct: 370 FSYLETNHATGKVVVYPIP 386

[Close]

BLAST of Tan0003728 vs. TAIR 10
Match: AT4G13010.1 (Oxidoreductase, zinc-binding dehydrogenase family protein )

HSP 1 Score: 143.3 bits (360), Expect = 3.4e-34
Identity = 117/336 (34.82%), Postives = 161/336 (47.92%), Query Frame = 0


Query: 10  MKAWVYHEYGNTADVLKLDSNFPVPQITQDQVLVKLVAASLNPIDFKRIHGALKPFHSSP 69
           M A  Y+ YG  A  L+     PVP    ++V +KL A SLNP+D+K   G ++PF    
Sbjct: 6   MHALQYNSYGGGAAGLE-HVQVPVPTPKSNEVCLKLEATSLNPVDWKIQKGMIRPFLPRK 65

Query: 70  -PYVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKTVGTLAEYTATEEKVLAL 129
            P +P  DVAG VV+VGS VK FK GD+V   ++H         G LAE+    EK+   
Sbjct: 66  FPCIPATDVAGEVVEVGSGVKNFKAGDKVVAVLSHLG------GGGLAEFAVATEKLTVK 125

Query: 130 KPKNLSFLEAASLPLAMETAYE------GLERVGFSAGKSILVLGGAGGVGAYVIQLAKH 189
           +P+ +   EAA+LP+A  TA +      GL+  G     +ILV   +GGVG Y +QLAK 
Sbjct: 126 RPQEVGAAEAAALPVAGLTALQALTNPAGLKLDGTGKKANILVTAASGGVGHYAVQLAK- 185

Query: 190 VFGASRIVATASTSKQDFLRSLGADHPIDYTKENFEDLPEKFDVVYDAV-----GQPDKA 249
               + + AT      +F++SLGAD  +DY       L       YDAV     G P   
Sbjct: 186 -LANAHVTATCGARNIEFVKSLGADEVLDYKTPEGAALKSPSGKKYDAVVHCANGIPFSV 245

Query: 250 VKA-IKEGGNIVSIAAPIPEFPDAF--------------FLLTSDAAMLEKLNPYLESGK 309
            +  + E G ++ I  P P     +               LL   A  LE +   ++ GK
Sbjct: 246 FEPNLSENGKVIDI-TPGPNAMWTYAVKKITMSKKQLVPLLLIPKAENLEFMVNLVKEGK 305

Query: 310 VKPIIDPKSPFPFSNTLEAFAYLETSRATGKIVVYP 319
           VK +ID K   P S   +A+A      ATGKI+V P
Sbjct: 306 VKTVIDSK--HPLSKAEDAWAKSIDGHATGKIIVEP 329

[Close]

BLAST of Tan0003728 vs. TAIR 10
Match: AT3G15090.1 (GroES-like zinc-binding alcohol dehydrogenase family protein )

HSP 1 Score: 131.0 bits (328), Expect = 1.8e-30
Identity = 100/348 (28.74%), Postives = 163/348 (46.84%), Query Frame = 0


Query: 5   SVPTHMKAWVYHEYGNTADVLKLDSNFPVPQITQDQVLVKLVAASLNPIDFK-RIHGALK 64
           SV T  +A +   +G   +V +L  N PVP +  ++VLVK  A S+NP+D + R      
Sbjct: 27  SVFTGCRAVILPRFGG-PEVFELRENVPVPNLNPNEVLVKAKAVSVNPLDCRIRAGYGRS 86

Query: 65  PFHSSPPYVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKTVGTLAEYTATEE 124
            F    P + G DV+G V  +G+ VK  KVG EV+G ++  AL      GT  +Y    E
Sbjct: 87  VFQPHLPIIVGRDVSGEVAAIGTSVKSLKVGQEVFGALHPTALR-----GTYTDYGILSE 146

Query: 125 KVLALKPKNLSFLEAASLPLAMETAYEGLE-RVGFSAGKSILVLGGAGGVGAYVIQLAKH 184
             L  KP ++S +EA+++P A  TA+  L+     + G+ +LV GG G VG   IQLA  
Sbjct: 147 DELTEKPSSISHVEASAIPFAALTAWRALKSNARITEGQRLLVFGGGGAVGFSAIQLA-- 206

Query: 185 VFGASRIVATASTSKQDFLRSLGADHPIDYTKENFE-DLPEKFDVVYDAVGQPDK---AV 244
           V     + A+     +D + + GA+  +DYT E+ E  +  KFD V D +G P+     +
Sbjct: 207 VASGCHVTASCVGQTKDRILAAGAEQAVDYTTEDIELAVKGKFDAVLDTIGGPETERIGI 266

Query: 245 KAIKEGGNIVSIAAPIPEFPDAF------------------------------FLLTSDA 304
             +++GGN +++        D +                                + +D 
Sbjct: 267 NFLRKGGNYMTLQGEAASLTDKYGFVVGLPLATSLLMKKKIQYQYSHGIDYWWTYMRADP 326

Query: 305 AMLEKLNPYLESGKVKPIIDPKSPFPFSNTLEAFAYLETSRATGKIVV 317
             L ++   + +GK+K  I  +  FP ++ + A    E  +  GK+V+
Sbjct: 327 EGLAEIQRLVGAGKLK--IPVEKTFPITDVVAAHEAKEKKQIPGKVVL 364

[Close]

BLAST of Tan0003728 vs. TAIR 10
Match: AT3G56460.1 (GroES-like zinc-binding alcohol dehydrogenase family protein )

HSP 1 Score: 110.5 bits (275), Expect = 2.5e-24
Identity = 80/255 (31.37%), Postives = 129/255 (50.59%), Query Frame = 0


Query: 19  GNTADVLKLDSNFPVPQITQD-QVLVKLVAASLNPIDFKRIHGALKPFHSSP--PYVPGY 78
           G+    +++    P+P +  D  V V+++A SLN  ++ +I G    +   P  P++PG 
Sbjct: 18  GSPESPVEVSKTHPIPSLNSDTSVRVRVIATSLNYANYLQILG---KYQEKPPLPFIPGS 77

Query: 79  DVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKTVGTLAEYTATEEKVLALKPKNLSF 138
           D +G+V  +G  V KF+VGD V    +         +G+ A++   ++  L L P+    
Sbjct: 78  DYSGIVDAIGPAVTKFRVGDRVCSFAD---------LGSFAQFIVADQSRLFLVPERCDM 137

Query: 139 LEAASLPLAMETAYEGL-ERVGFSAGKSILVLGGAGGVGAYVIQLAKHVFGASRIVATAS 198
           + AA+LP+A  T++  L  R   ++G+ +LVLG AGGVG   +Q+ K V GA  I     
Sbjct: 138 VAAAALPVAFGTSHVALVHRARLTSGQVLLVLGAAGGVGLAAVQIGK-VCGAIVIAVARG 197

Query: 199 TSKQDFLRSLGADHPIDYTKENFEDLPEKF---------DVVYDAVG--QPDKAVKAIKE 258
           T K   L+S+G DH +D   EN     ++F         DV+YD VG     +++K +K 
Sbjct: 198 TEKIQLLKSMGVDHVVDLGTENVISSVKEFIKTRKLKGVDVLYDPVGGKLTKESMKVLKW 257

[Close]

BLAST of Tan0003728 vs. TAIR 10
Match: AT4G21580.1 (oxidoreductase, zinc-binding dehydrogenase family protein )

HSP 1 Score: 102.1 bits (253), Expect = 8.7e-22
Identity = 96/340 (28.24%), Postives = 156/340 (45.88%), Query Frame = 0


Query: 10  MKAWVYHEYGNTADVLKLDSNFPVPQITQDQVLVKLVAASLNPIDFKRIHGALKPFHSSP 69
           MKA V  E G   +VL+L  +   P++  D+VL++++A +LN  D  +  G   P   S 
Sbjct: 1   MKAIVISEPGK-PEVLQL-RDVADPEVKDDEVLIRVLATALNRADTLQRLGLYNPPPGSS 60

Query: 70  PYVPGYDVAGVVVKVGSEVKKFKVGDEVYGDINHKALDKPKTVGTLAEYTATEEKVLALK 129
           PY+ G + +G +  VG  V ++KVGD+V   ++          G  AE  +     +   
Sbjct: 61  PYL-GLECSGTIESVGKGVSRWKVGDQVCALLSG---------GGYAEKVSVPAGQIFPI 120

Query: 130 PKNLSFLEAASLPLAMETAYEGLERVG-FSAGKSILVLGGAGGVGAYVIQLAKHVFGASR 189
           P  +S  +AA+ P    T +  +  +G  S G+S L+ GG+ G+G + IQ+AKH+ G   
Sbjct: 121 PAGISLKDAAAFPEVACTVWSTVFMMGRLSVGESFLIHGGSSGIGTFAIQIAKHL-GVRV 180

Query: 190 IVATASTSKQDFLRSLGADHPIDYTKENF------EDLPEKFDVVYDAVGQP--DKAVKA 249
            V   S  K    + LGAD  I+Y  E+F      E   +  DV+ D +G P   K + +
Sbjct: 181 FVTAGSDEKLAACKELGADVCINYKTEDFVAKVKAETDGKGVDVILDCIGAPYLQKNLDS 240

Query: 250 IKEGGNIVSI------------------------AAPIPEFPDAFFLLTSDAAMLEKLNP 309
           +   G +  I                        AA  P  P+   ++  +    + + P
Sbjct: 241 LNFDGRLCIIGLMGGANAEIKLSSLLPKRLTVLGAALRPRSPENKAVVVREVE--KNVWP 300

Query: 310 YLESGKVKPIIDPKSPFPFSNTLEAFAYLETSRATGKIVV 317
            +E+GKVKP+I      P S   E  + +E+S   GKI++
Sbjct: 301 AIEAGKVKPVI--YKYLPLSQAAEGHSLMESSNHIGKILL 323

The following BLAST results are available for this feature:

BLAST of Tan0003728 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q84V25	4.2e-122	69.94	2-methylene-furan-3-one reductase OS=Fragaria ananassa OX=3747 GN=EO PE=1 SV=1	[more]
Q941I0	8.0e-121	69.72	2-methylene-furan-3-one reductase OS=Fragaria ananassa OX=3747 GN=EO PE=1 SV=2	[more]
O23939	1.0e-120	69.30	2-methylene-furan-3-one reductase OS=Fragaria vesca OX=57918 GN=EO PE=2 SV=2	[more]
K4BW79	2.0e-119	67.92	2-methylene-furan-3-one reductase OS=Solanum lycopersicum OX=4081 GN=EO PE=1 SV=...	[more]
Q9ZUC1	1.0e-112	64.89	NADPH-dependent alkenal/one oxidoreductase, chloroplastic OS=Arabidopsis thalian...	[more]

BLAST of Tan0003728 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022961926.1	4.0e-162	91.56	2-methylene-furan-3-one reductase-like [Cucurbita moschata] >KAG6602443.1 2-meth...	[more]
XP_022990587.1	2.6e-161	91.25	2-methylene-furan-3-one reductase-like [Cucurbita maxima]	[more]
XP_038890251.1	5.8e-161	89.38	2-methylene-furan-3-one reductase-like [Benincasa hispida]	[more]
XP_023543317.1	2.1e-158	90.00	2-methylene-furan-3-one reductase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022133822.1	5.1e-157	87.19	2-methylene-furan-3-one reductase-like [Momordica charantia]	[more]

BLAST of Tan0003728 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HBQ4	2.0e-162	91.56	2-methylene-furan-3-one reductase-like OS=Cucurbita moschata OX=3662 GN=LOC11146...	[more]
A0A6J1JJ69	1.3e-161	91.25	2-methylene-furan-3-one reductase-like OS=Cucurbita maxima OX=3661 GN=LOC1114874...	[more]
A0A6J1BXV5	2.5e-157	87.19	2-methylene-furan-3-one reductase-like OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1FK96	7.2e-157	87.81	2-methylene-furan-3-one reductase-like OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1JTD8	1.2e-156	87.81	2-methylene-furan-3-one reductase-like OS=Cucurbita maxima OX=3661 GN=LOC1114895...	[more]

BLAST of Tan0003728 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G23740.1	7.5e-114	64.89	Oxidoreductase, zinc-binding dehydrogenase family protein	[more]
AT4G13010.1	3.4e-34	34.82	Oxidoreductase, zinc-binding dehydrogenase family protein	[more]
AT3G15090.1	1.8e-30	28.74	GroES-like zinc-binding alcohol dehydrogenase family protein	[more]
AT3G56460.1	2.5e-24	31.37	GroES-like zinc-binding alcohol dehydrogenase family protein	[more]
AT4G21580.1	8.7e-22	28.24	oxidoreductase, zinc-binding dehydrogenase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR020843	Polyketide synthase, enoylreductase domain	SMART	SM00829	PKS_ER_names_mod	coord: 20..316 e-value: 1.1E-13 score: 42.8
None	No IPR available	PFAM	PF13602	ADH_zinc_N_2	coord: 204..316 e-value: 2.2E-20 score: 74.1
None	No IPR available	GENE3D	3.90.180.10		coord: 17..316 e-value: 8.0E-109 score: 365.4
None	No IPR available	GENE3D	3.40.50.720		coord: 152..267 e-value: 8.0E-109 score: 365.4
None	No IPR available	PANTHER	PTHR44573:SF3	CYTOSOLIC ALKENAL/ONE OXIDOREDUCTASE	coord: 3..320
None	No IPR available	CDD	cd05289	MDR_like_2	coord: 10..316 e-value: 8.28332E-103 score: 301.787
IPR013154	Alcohol dehydrogenase, N-terminal	PFAM	PF08240	ADH_N	coord: 39..101 e-value: 2.7E-10 score: 40.1
IPR044626	NADPH-dependent oxidoreductase AOR-like	PANTHER	PTHR44573	NADPH-DEPENDENT ALKENAL/ONE OXIDOREDUCTASE, CHLOROPLASTIC	coord: 3..320
IPR002364	Quinone oxidoreductase/zeta-crystallin, conserved site	PROSITE	PS01162	QOR_ZETA_CRYSTAL	coord: 160..181
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 133..287
IPR011032	GroES-like superfamily	SUPERFAMILY	50129	GroES-like	coord: 6..167

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0003728.1	Tan0003728.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016628	oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor
molecular_function	GO:0008270	zinc ion binding
molecular_function	GO:0016491	oxidoreductase activity