Tan0003703 (gene) Snake gourd v1

Overview


Name	Tan0003703
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	beta-carotene hydroxylase 2, chloroplastic-like
Location	LG01: 1800328 .. 1803608 (+)
RNA-Seq Expression	Tan0003703
Synteny	Tan0003703

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCGAAATAAAAAAATTAAATCAAAATATGAAAAAGAAAATGATCGACTTTACGACCTCTTAACTAAAAATTACGAAAAAAGAAAAGTTAATAAATGAAAAAATAAAATAAAATTTATAAAAGAGAGAGAGAAATAGAAAAAATGGCAATTTCGTATATAAAGGAAGGAAGGGCAGCAAATGCTCCAACAAACTACTTCCCCATTGTTTTTTGCTCAGAGTTTTTACTCCACCACTTTACTGACAAAAACTTGTGGTCTCTGTTTCTTCAAGCTCCATTTTTAAGCCCTCTCGCCGCCGTCTCTTTCTCCGAGAAGCTTCCCCTTCTTCCACCGTCTTTTCACTTTCATCTCTCCCATGGCGGCCGGCCTCTCCGCCGCCTTAGGGCCCAAACCATTCCATCTCTTCCTTACTTCCTCCAATCTCTCCCCTAAATCCCGAACTCCGTTTCTGTTTCCACGTTCAGTCTTCCGGAACAGTAGAATCCAATGGAAGATGCGGAGAAGAACGCTGCTCACCGTCTGCGTGTTCGTTGAGGATCAGAATAGTTCCGGCGAGGTGGAGAATCTCGCCGACGAAGGATCGCCGATTATAGTCCCTCAGATCCCATCGCCTCATGTTGCAGAGAGATTGGCGAGGAAGAGATCGGAGCGCTTCACTTATCTTGTTGCTGCGGTTATGTCTAGTTTTGGAATTACCTCCATGGCTGTCATGGCGGTTTACTACCGATTTTACTGGCAAATGGAGGTCCTCTCTCTCTATCTCTCTATAGAAACTTGAATTGTTTTTGTTTTCATTGGCGCCGTATTATTCTGACTGAATATTGAATTCCGATTGTTTAATTTGGCGGTGTGTAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTTCTCTCTCCGTTGGTGCCGCTGTACGTTTTCAATTTCATTTCATCTCTGTATTCTCTTCTTTTCTCGAAGAATTCGATTTTCTGTAGGCTGATTTTCACAATCAATTTGGGAATATTCAGGTCGGAATGGAGTTTTGGGCAAGATGGGCGCATAGGGCTCTCTGGCACGCTTCCTTGTGGCATATGCACGAGGTATAGGTTTCTAATTCTTCTTCTCAATAGAATTTCGTTTCTGTGTTTGTCTGCTGAGAAAATTTCTATCAACAATAATCCACAATGAATCTTAATTTCATATCAAACAAACAATATTCAAAAGGGAAAAGAGAATCGTTGAGGCTTGGACTTGTTGATTGCAGCACAGTCCTTGCACTATTCCTAAACTAATCCTCCTCTCTGTAGAATTTTTCTTGTTTGGCTGCTGAGAAAATTCAATTAACAATGGACCTTTCTCTCATTTTCCTAGCAAACAAACAACATTGAATAAAAAAAGAAAAATCGTTGACGCTTGGACTTTTTTTTTTTTGGTTGATCTTTATTATTACAGTCTCACCATAAACCAAGAGAAGGACCGTTCGAATTGAACGATGTTTTCGCCATTATCAACGCCGTGCCCGCCATAGCTCTCCTTTCTTACGGCTTCTTCCATAAAGGCCTCGTCCCTGGTCTCTGCTTCGGCGCTGTAAATCTCCATATCTTTCTTCAATTTCTCTCTCATAATCTTTCAGAATCTCAGTTTGTTCATATTCTAACTATTTAATAGTTATTTAATCACTGGGGATTAGCGCTAATGACAGTGTTAAATGACGAAAATACCCCTAGAAAATGTGGTAATTGAGAAAGAGGTTTGACACGCTGGCGGGTTGTGTTGTGGCAGGGCCTTGGAATTACGGTCTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCTTGTGGGTCCCATTGCCAACGTGCCCTATTTTAGAAAGGTCGCTGCTGCTCACCAGGTAAATTCAATTTCTAAATTTTTATTTCTTTTTAATTTTTTTTAATGCTTAATCTTCATTCTTCCCTTAAAAAATTATCAAATTTGTTATAATTTTTATCTTATTTTGATCTCAAACTTTTGATACTGACTTTGTTTGTTCCCTTGTGTTGACTTTTCATTTAACAAAAACTCAATTATTTTTCCTAACTTTATATTTTATTTGGATCCCTAGACGTACATTTAAGATGATATTTTTTTTAAGTTGAAATCTTCATTCTCATATTTATTGTACAGGTAAATTCCATGTAGATTTCTATTAATGAAATATATGAATCTAAATATATATATATATGAAAAAACTTATTAAATAAAATTTTAAAATGTCAATTGTTTTAAAAAAAAATTAAGAATATTGAGAAATGTAGAGATAAGAAAGAACCTATAAACTTAGGGGGCGGGGGGGAATATGATTTTAAATCAACCGACTAATAAGAAATTAGCTATGGAGTATGAAAATATAATAATATTCTTAGAGTTTGGGCAATTTTTAATAATCTCGCATTAATTTATGTTTTTACGGCTGAAATTAAATACATGTGTTTTTTTTTTTTGGAGACAAAAAACATGTGTTATTAACCTGCACGTAGAAAAGTAATTACACGTATCTAAGTGTAAATCTAATTTTAAAAGATTATGTCCGATTTAAATATTGAAGCTTATGGTTTTTTTTTTCTGGCTGTAGCTTCACCATTCGGACAAGTTCAACGGTGTGCCCTACGGGCTGTTTTTGGGTCCGAAGGTCAGTTTCGGGTCTATTTATTTATCTTAAATTTTAAATTTATGGTCTTTTTTTTTTTTAATAAATGGTTTCCTAAAAAATGTTGTGGCGTTTTTTTTAATAAATGGTTTTCTCAAAAATGTTGTGGTGCAGGAACTAGAGGAAGTGGGAGGCCAAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATCAACGGCCACAAAATCAATCGACGGCTCTTGATTAAAGTCGATAGAGGGTTTCACTATTAATATTATGTAAAAATGAGAAGTAAAAGGGTAGAAGAATCAAGAATGAGATAGTAAATTTGGTAGAAAGGATTCGCTTTTACCGAGGAATTACCCTCTACTTTTTAGTTTTTACGGTCTAATGTGATTGTGAAGAAAAAAGAAGTCAGCCGAGAGTAAATAACACTCCATAATTTTGTGTAATTTTCGATATTTCATAATTTAATAAAATATGTAGTCATTTGAGTAGGTAAAATGGGAAAATTTCTAGTTCTACGTAGTAGGCACAATTTTTAATGTGTTTACCATTTTAAACTAATTTTTATTTGGGTCCCACCCATTTAAATAACATACACTTACACTCTCATCTCCCCAC

mRNA sequence

CTCGAAATAAAAAAATTAAATCAAAATATGAAAAAGAAAATGATCGACTTTACGACCTCTTAACTAAAAATTACGAAAAAAGAAAAGTTAATAAATGAAAAAATAAAATAAAATTTATAAAAGAGAGAGAGAAATAGAAAAAATGGCAATTTCGTATATAAAGGAAGGAAGGGCAGCAAATGCTCCAACAAACTACTTCCCCATTGTTTTTTGCTCAGAGTTTTTACTCCACCACTTTACTGACAAAAACTTGTGGTCTCTGTTTCTTCAAGCTCCATTTTTAAGCCCTCTCGCCGCCGTCTCTTTCTCCGAGAAGCTTCCCCTTCTTCCACCGTCTTTTCACTTTCATCTCTCCCATGGCGGCCGGCCTCTCCGCCGCCTTAGGGCCCAAACCATTCCATCTCTTCCTTACTTCCTCCAATCTCTCCCCTAAATCCCGAACTCCGTTTCTGTTTCCACGTTCAGTCTTCCGGAACAGTAGAATCCAATGGAAGATGCGGAGAAGAACGCTGCTCACCGTCTGCGTGTTCGTTGAGGATCAGAATAGTTCCGGCGAGGTGGAGAATCTCGCCGACGAAGGATCGCCGATTATAGTCCCTCAGATCCCATCGCCTCATGTTGCAGAGAGATTGGCGAGGAAGAGATCGGAGCGCTTCACTTATCTTGTTGCTGCGGTTATGTCTAGTTTTGGAATTACCTCCATGGCTGTCATGGCGGTTTACTACCGATTTTACTGGCAAATGGAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTTCTCTCTCCGTTGGTGCCGCTGTCGGAATGGAGTTTTGGGCAAGATGGGCGCATAGGGCTCTCTGGCACGCTTCCTTGTGGCATATGCACGAGTCTCACCATAAACCAAGAGAAGGACCGTTCGAATTGAACGATGTTTTCGCCATTATCAACGCCGTGCCCGCCATAGCTCTCCTTTCTTACGGCTTCTTCCATAAAGGCCTCGTCCCTGGTCTCTGCTTCGGCGCTGGCCTTGGAATTACGGTCTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCTTGTGGGTCCCATTGCCAACGTGCCCTATTTTAGAAAGGTCGCTGCTGCTCACCAGCTTCACCATTCGGACAAGTTCAACGGTGTGCCCTACGGGCTGTTTTTGGGTCCGAAGGAACTAGAGGAAGTGGGAGGCCAAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATCAACGGCCACAAAATCAATCGACGGCTCTTGATTAAAGTCGATAGAGGGTTTCACTATTAATATTATGTAAAAATGAGAAGTAAAAGGGTAGAAGAATCAAGAATGAGATAGTAAATTTGGTAGAAAGGATTCGCTTTTACCGAGGAATTACCCTCTACTTTTTAGTTTTTACGGTCTAATGTGATTGTGAAGAAAAAAGAAGTCAGCCGAGAGTAAATAACACTCCATAATTTTGTGTAATTTTCGATATTTCATAATTTAATAAAATATGTAGTCATTTGAGTAGGTAAAATGGGAAAATTTCTAGTTCTACGTAGTAGGCACAATTTTTAATGTGTTTACCATTTTAAACTAATTTTTATTTGGGTCCCACCCATTTAAATAACATACACTTACACTCTCATCTCCCCAC

Coding sequence (CDS)

ATGGCGGCCGGCCTCTCCGCCGCCTTAGGGCCCAAACCATTCCATCTCTTCCTTACTTCCTCCAATCTCTCCCCTAAATCCCGAACTCCGTTTCTGTTTCCACGTTCAGTCTTCCGGAACAGTAGAATCCAATGGAAGATGCGGAGAAGAACGCTGCTCACCGTCTGCGTGTTCGTTGAGGATCAGAATAGTTCCGGCGAGGTGGAGAATCTCGCCGACGAAGGATCGCCGATTATAGTCCCTCAGATCCCATCGCCTCATGTTGCAGAGAGATTGGCGAGGAAGAGATCGGAGCGCTTCACTTATCTTGTTGCTGCGGTTATGTCTAGTTTTGGAATTACCTCCATGGCTGTCATGGCGGTTTACTACCGATTTTACTGGCAAATGGAGGGCGGAGAGATTCCTTTCTCTGAAATGTTTGGTACATTTTCTCTCTCCGTTGGTGCCGCTGTCGGAATGGAGTTTTGGGCAAGATGGGCGCATAGGGCTCTCTGGCACGCTTCCTTGTGGCATATGCACGAGTCTCACCATAAACCAAGAGAAGGACCGTTCGAATTGAACGATGTTTTCGCCATTATCAACGCCGTGCCCGCCATAGCTCTCCTTTCTTACGGCTTCTTCCATAAAGGCCTCGTCCCTGGTCTCTGCTTCGGCGCTGGCCTTGGAATTACGGTCTTTGGGATGGCCTACATGTTCGTCCACGACGGTCTCGTTCACAAAAGATTCCTTGTGGGTCCCATTGCCAACGTGCCCTATTTTAGAAAGGTCGCTGCTGCTCACCAGCTTCACCATTCGGACAAGTTCAACGGTGTGCCCTACGGGCTGTTTTTGGGTCCGAAGGAACTAGAGGAAGTGGGAGGCCAAGAAGAATTGGAGAAGGAAATCAACAGAAGAATAAAATCAACGGCCACAAAATCAATCGACGGCTCTTGA

Protein sequence

MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVEDQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIKSTATKSIDGS

Homology

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BLAST of Tan0003703 vs. ExPASy Swiss-Prot
Match: O49814 (Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=1 SV=2)

HSP 1 Score: 435.3 bits (1118), Expect = 5.9e-121
Identity = 218/287 (75.96%), Postives = 238/287 (82.93%), Query Frame = 0


Query: 25  PKSRTPFLFPRSVFR-NSRIQWKMRRRTLLTVCVFVEDQNSSGEVENLADEGSPIIVPQI 84
           P   TP ++P + F  N     + RRR   TVC  +ED     + E   ++    I  QI
Sbjct: 27  PTPTTPSVYPITPFSPNLGSILRCRRRPSFTVCFVLEDDKFKTQFEAGEEDIEMKIEEQI 86

Query: 85  PSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEIPFSEMFGTF 144
            +  +AE+LARK+SERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGE+PFSEMFGTF
Sbjct: 87  SATRLAEKLARKKSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEVPFSEMFGTF 146

Query: 145 SLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIALLS 204
           +LSVGAAVGMEFWARWAH+ALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIALL 
Sbjct: 147 ALSVGAAVGMEFWARWAHKALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIALLD 206

Query: 205 YGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFLVGPIANVPYFRKVAAAHQLH 264
           YGFFHKGL+PGLCFGAGLGITVFGMAYMFVHDGLVHKRF VGP+ANVPY RKVAAAH LH
Sbjct: 207 YGFFHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPVANVPYLRKVAAAHSLH 266

Query: 265 HSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIKSTATKSIDGS 311
           HS+KFNGVPYGLFLGPKELEEVGG EELEKE+NRR     T+ I GS
Sbjct: 267 HSEKFNGVPYGLFLGPKELEEVGGLEELEKEVNRR-----TRYIKGS 308

[Close]

BLAST of Tan0003703 vs. ExPASy Swiss-Prot
Match: B3SGL0 (Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=2 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 1.7e-115
Identity = 213/297 (71.72%), Postives = 242/297 (81.48%), Query Frame = 0


Query: 18  LTSSNLSPKS-----RTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVEDQNSSGE--VEN 77
           ++SS+L+PKS      TP L     F+      + R +  LTVC  +E++   G+  V +
Sbjct: 21  ISSSSLTPKSSPVSTSTPTLVVFPPFKLVSKSLRTRSKPRLTVCFVLEEKELRGKLVVAS 80

Query: 78  LADEGSPIIVPQ------IPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMAVYYR 137
             D+G+  +  Q        +  +A++LARK+SERFTYLVAAVMSSFGITSMAV++VYYR
Sbjct: 81  DDDDGAGEVRKQREKEISASAEKLAQKLARKKSERFTYLVAAVMSSFGITSMAVLSVYYR 140

Query: 138 FYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREGPF 197
           F WQMEGGEIP SEMFGTF+LSVGAAVGMEFWARWAH ALWHASLWHMHESHHKPREGPF
Sbjct: 141 FSWQMEGGEIPLSEMFGTFALSVGAAVGMEFWARWAHEALWHASLWHMHESHHKPREGPF 200

Query: 198 ELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFLV 257
           ELND+FAIINAVPAIALLSYGFFHKGL+PGLCFGAGLGITVFGMAYMFVHDGLVHKRF V
Sbjct: 201 ELNDIFAIINAVPAIALLSYGFFHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPV 260

Query: 258 GPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIKS 302
           GPIA+VPYFR+VAAAH LHHSDKFNGVPYGLFLGPKELEEVGG + LE EINRR K+
Sbjct: 261 GPIADVPYFRRVAAAHTLHHSDKFNGVPYGLFLGPKELEEVGGLQVLEMEINRRTKN 317

[Close]

BLAST of Tan0003703 vs. ExPASy Swiss-Prot
Match: Q9SZZ8 (Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 1 PE=1 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 3.5e-113
Identity = 215/314 (68.47%), Postives = 245/314 (78.03%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLS A+  KP H   +SS+   + R     P+S+   S       R    +VC  VE
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLR----LPKSLSGFS----PSLRFKRFSVCYVVE 60

Query: 61  DQNSSGEVEN----LADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSM 120
           ++  +  +EN     +   +  I  +  +  +AE+L RK+SER TYL+AA++SSFGITSM
Sbjct: 61  ERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGITSM 120

Query: 121 AVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESH 180
           AVMAVYYRF WQMEGGEI   EMFGTF+LSVGAAVGMEFWARWAHRALWHASLW+MHESH
Sbjct: 121 AVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMHESH 180

Query: 181 HKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDG 240
           HKPREGPFELNDVFAI+NA PAI LLSYGFF+KGLVPGLCFGAGLGITVFG+AYMFVHDG
Sbjct: 181 HKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGAGLGITVFGIAYMFVHDG 240

Query: 241 LVHKRFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEIN 300
           LVHKRF VGPIA+VPY RKVAAAHQLHH+DKFNGVPYGLFLGPKELEEVGG EEL+KEI+
Sbjct: 241 LVHKRFPVGPIADVPYLRKVAAAHQLHHTDKFNGVPYGLFLGPKELEEVGGNEELDKEIS 300

Query: 301 RRIKSTATKSIDGS 311
           RRIKS    S  GS
Sbjct: 301 RRIKSYKKASGSGS 306

[Close]

BLAST of Tan0003703 vs. ExPASy Swiss-Prot
Match: O49815 (Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=1 SV=2)

HSP 1 Score: 399.8 bits (1026), Expect = 2.8e-110
Identity = 201/281 (71.53%), Postives = 229/281 (81.49%), Query Frame = 0


Query: 30  PFLFPRSVFRNSRIQWKMRRRTLLTVCVFVEDQN----SSGEVENLADEGSPIIV----P 89
           P LF   +  N     + RR+  L  C  ++D       SG+  +    G  I V     
Sbjct: 32  PLLFFSPLTCNLDAILRSRRKPRLAACFVLKDDKLYTAQSGKQSDTEAIGDEIEVETNEE 91

Query: 90  QIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMAVYYRFYWQMEGGEIPFSEMFG 149
           +  +  +AE+ ARK+SERFTYLVAAVMSS GITSMAV++VYYRF WQMEGGE+PFSEMF 
Sbjct: 92  KSLAVRLAEKFARKKSERFTYLVAAVMSSLGITSMAVISVYYRFSWQMEGGEMPFSEMFC 151

Query: 150 TFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPREGPFELNDVFAIINAVPAIAL 209
           TF+L+ GAA+GME+WARWAHRALWHASLWHMHESHH+PREGPFELND+FAIINAVPAIAL
Sbjct: 152 TFALAFGAAIGMEYWARWAHRALWHASLWHMHESHHRPREGPFELNDIFAIINAVPAIAL 211

Query: 210 LSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHKRFLVGPIANVPYFRKVAAAHQ 269
           LS+GF HKGL+PGLCFGAGLGITVFGMAYMFVHDGLVHKRF VGPIANVPYF++VAAAHQ
Sbjct: 212 LSFGFNHKGLIPGLCFGAGLGITVFGMAYMFVHDGLVHKRFPVGPIANVPYFQRVAAAHQ 271

Query: 270 LHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIKST 303
           LHHSDKF+GVPYGLFLGPKELEEVG  EELEKE+NRRIKS+
Sbjct: 272 LHHSDKFDGVPYGLFLGPKELEEVGVLEELEKEVNRRIKSS 312

[Close]

BLAST of Tan0003703 vs. ExPASy Swiss-Prot
Match: Q9LTG0 (Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=BETA-OHASE 2 PE=2 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 4.0e-109
Identity = 209/305 (68.52%), Postives = 239/305 (78.36%), Query Frame = 0


Query: 1   MAAGLSA-ALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFV 60
           MAAGLS  A+  KP +    S+N  P S    +FP S+  N       RRR +LTVC  V
Sbjct: 1   MAAGLSTIAVTLKPLNRSSFSAN-HPISTA--VFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQNSSGEVENLADEGSPIIVPQ----IPSPHVAERLARKRSERFTYLVAAVMSSFGITS 120
           E++  S  ++   D+  P         + +  + ++  +K+SERFTYL+AAVMSSFGITS
Sbjct: 61  EERKQSSPMD---DDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITS 120

Query: 121 MAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHES 180
           MA+MAVYYRF WQM+GGE+   EMFGTF+LSVGAAVGMEFWARWAHRALWH SLW+MHES
Sbjct: 121 MAIMAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHES 180

Query: 181 HHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHD 240
           HHKPREG FELNDVFAI NAVPAI LL YGF +KGLVPGLCFGAGLGIT+FGMAYMFVHD
Sbjct: 181 HHKPREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGITMFGMAYMFVHD 240

Query: 241 GLVHKRFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEI 300
           GLVHKRF VGPIANVPY RKVAAAHQLHH+DKF GVPYGLFLGPKE+EEVGG+EELEKEI
Sbjct: 241 GLVHKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEI 294

[Close]

BLAST of Tan0003703 vs. NCBI nr
Match: XP_038877448.1 (beta-carotene hydroxylase 2, chloroplastic [Benincasa hispida])

HSP 1 Score: 588.2 bits (1515), Expect = 4.2e-164
Identity = 291/310 (93.87%), Postives = 296/310 (95.48%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLSAAL PKP HL LTSS+LSPK R PFLFP SVFRNSRIQWKMRRRTL TVCV VE
Sbjct: 1   MAAGLSAALVPKPLHLLLTSSHLSPKPRIPFLFPPSVFRNSRIQWKMRRRTLFTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQNSSGEVENL+DE SPIIVPQIPSPHV+ERLARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQNSSGEVENLSDEVSPIIVPQIPSPHVSERLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWH+SLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RF VGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGG EELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 301 STATKSIDGS 311
             A KSIDGS
Sbjct: 301 WAAPKSIDGS 310

[Close]

BLAST of Tan0003703 vs. NCBI nr
Match: KAG7013258.1 (Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 587.0 bits (1512), Expect = 9.3e-164
Identity = 288/310 (92.90%), Postives = 298/310 (96.13%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLSA LGPKPFH+FLTSSNLSPK RTPFLFP SVFRNSRIQWKMRR+TLLTVCV VE
Sbjct: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENLADE  PIIVPQIPSPHVAE+LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RF VGPIANVPYFRKVAAAHQLHHS+KFNGVPYGLFLGPKELEEVGG EELEKEI+RRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEISRRIK 300

Query: 301 STATKSIDGS 311
           STA K I+GS
Sbjct: 301 STAKKPINGS 310

[Close]

BLAST of Tan0003703 vs. NCBI nr
Match: XP_022944865.1 (beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 585.9 bits (1509), Expect = 2.1e-163
Identity = 287/310 (92.58%), Postives = 297/310 (95.81%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLSA LGPKPFH+FLTSSNLSPK RTPFLFP SVFRNSRIQWKMRR+TLLTVCV VE
Sbjct: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENLADE  PIIVPQIPSPHVAE+LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RF VGPIANVPYFRKVAAAHQLHHS+KFNGVPYGLFLGPKELEEVGG +ELEKEINRR K
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLQELEKEINRRTK 300

Query: 301 STATKSIDGS 311
           STA K I+GS
Sbjct: 301 STAKKPINGS 310

[Close]

BLAST of Tan0003703 vs. NCBI nr
Match: KAG6574199.1 (Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 583.9 bits (1504), Expect = 7.9e-163
Identity = 287/310 (92.58%), Postives = 297/310 (95.81%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLSA LGPK FH+FLTSSNLSPK RTPFLFP SVFRNSRIQWKMRR+TLLTVCV VE
Sbjct: 1   MAAGLSAVLGPKSFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENLADE  PIIVPQIPSPHVAE+LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RF VGPIANVPYFRKVAAAHQLHHS+KFNGVPYGLFLGPKELEEVGG EELEKEI+RRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEISRRIK 300

Query: 301 STATKSIDGS 311
           STA K I+GS
Sbjct: 301 STAKKPINGS 310

[Close]

BLAST of Tan0003703 vs. NCBI nr
Match: XP_023541483.1 (beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 583.2 bits (1502), Expect = 1.3e-162
Identity = 285/310 (91.94%), Postives = 297/310 (95.81%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAA +SA LGPKPFH+FLTSSNLSPK RTPFL+P SVFR+SRIQWKMRR+TLLTVCV VE
Sbjct: 1   MAASVSAVLGPKPFHVFLTSSNLSPKPRTPFLYPSSVFRSSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENLADE  PIIVPQIPSPHVAE+LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADENLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RF VGPIANVPYFRKVAAAHQLHHS+KFNGVPYGLFLGPKELEEVGG EELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 301 STATKSIDGS 311
           STA K I+GS
Sbjct: 301 STAKKPINGS 310

[Close]

BLAST of Tan0003703 vs. ExPASy TrEMBL
Match: A0A6J1FZ94 (beta-carotene hydroxylase 2, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449265 PE=3 SV=1)

HSP 1 Score: 585.9 bits (1509), Expect = 1.0e-163
Identity = 287/310 (92.58%), Postives = 297/310 (95.81%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLSA LGPKPFH+FLTSSNLSPK RTPFLFP SVFRNSRIQWKMRR+TLLTVCV VE
Sbjct: 1   MAAGLSAVLGPKPFHVFLTSSNLSPKPRTPFLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENLADE  PIIVPQIPSPHVAE+LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLPIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RF VGPIANVPYFRKVAAAHQLHHS+KFNGVPYGLFLGPKELEEVGG +ELEKEINRR K
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKFNGVPYGLFLGPKELEEVGGLQELEKEINRRTK 300

Query: 301 STATKSIDGS 311
           STA K I+GS
Sbjct: 301 STAKKPINGS 310

[Close]

BLAST of Tan0003703 vs. ExPASy TrEMBL
Match: A0A0A0L606 (Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G183920 PE=3 SV=1)

HSP 1 Score: 582.4 bits (1500), Expect = 1.1e-162
Identity = 285/310 (91.94%), Postives = 297/310 (95.81%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLSAA  PKPFHLFLTSS+LSPK  +PFLFP SVFRNSR QWKMRR+TLLTVCV VE
Sbjct: 1   MAAGLSAATLPKPFHLFLTSSHLSPKPPSPFLFPPSVFRNSRFQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
            QNSSG+V+NL+DE SPI+VPQIPSPHV+ERLARK+SERFTYLVAAVMS+FGITSMAVMA
Sbjct: 61  GQNSSGKVDNLSDEESPIVVPQIPSPHVSERLARKKSERFTYLVAAVMSTFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWH+SLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELND+FAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDIFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RF VGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGG EELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 301 STATKSIDGS 311
            TA KSIDGS
Sbjct: 301 WTARKSIDGS 310

[Close]

BLAST of Tan0003703 vs. ExPASy TrEMBL
Match: A0A6J1E4L5 (beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111430578 PE=3 SV=1)

HSP 1 Score: 581.3 bits (1497), Expect = 2.5e-162
Identity = 285/310 (91.94%), Postives = 296/310 (95.48%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAA +S ALGP+P HLFL SS+LSPK RTPFLFP SVFRNSRIQ K+RR+TL TVCV VE
Sbjct: 1   MAAAISGALGPRPLHLFLPSSHLSPKPRTPFLFPPSVFRNSRIQQKIRRKTLFTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQNSSGEVE+L+DE  PI+VPQIPSP+VA+RLARKRSERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQNSSGEVESLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWH+SLWHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHSSLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RF VGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300

Query: 301 STATKSIDGS 311
            T TKSIDGS
Sbjct: 301 LTTTKSIDGS 310

[Close]

BLAST of Tan0003703 vs. ExPASy TrEMBL
Match: A0A6J1J1S8 (beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111482629 PE=3 SV=1)

HSP 1 Score: 580.1 bits (1494), Expect = 5.5e-162
Identity = 286/310 (92.26%), Postives = 295/310 (95.16%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAA LSAALGPKP HLFL SS+LS K RTPFLFP SVFRNSRIQ KMRR+TL TVCV VE
Sbjct: 1   MAAALSAALGPKPLHLFLASSHLSHKPRTPFLFPPSVFRNSRIQRKMRRKTLFTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQNSSGEVENL+DE  PI+VPQIPSP+VA+RLARKRSERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQNSSGEVENLSDEEPPIVVPQIPSPYVAQRLARKRSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRFYWQMEGGEIPFSEMFGTF LSVGAAVGMEFWARWAHRALWH+S+WHMHESHHKPR
Sbjct: 121 VYYRFYWQMEGGEIPFSEMFGTFCLSVGAAVGMEFWARWAHRALWHSSMWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RF VGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300

Query: 301 STATKSIDGS 311
            T TKSI+GS
Sbjct: 301 LTTTKSINGS 310

[Close]

BLAST of Tan0003703 vs. ExPASy TrEMBL
Match: A0A6J1HX82 (beta-carotene hydroxylase 2, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111467695 PE=3 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 2.1e-161
Identity = 285/310 (91.94%), Postives = 294/310 (94.84%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAA LSA LGPKPFH+FLTSSNLSPK RTP LFP SVFRNSRIQWKMRR+TLLTVCV VE
Sbjct: 1   MAASLSAVLGPKPFHVFLTSSNLSPKPRTPLLFPSSVFRNSRIQWKMRRKTLLTVCVLVE 60

Query: 61  DQNSSGEVENLADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSMAVMA 120
           DQ+SS EVENLADE   IIVPQIPSPHVAE+LARK+SERFTYLVAAVMSSFGITSMAVMA
Sbjct: 61  DQSSSSEVENLADEKLLIIVPQIPSPHVAEKLARKKSERFTYLVAAVMSSFGITSMAVMA 120

Query: 121 VYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180
           VYYRF+WQMEGGEIPFSEMFGTF+LSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR
Sbjct: 121 VYYRFHWQMEGGEIPFSEMFGTFALSVGAAVGMEFWARWAHRALWHASLWHMHESHHKPR 180

Query: 181 EGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240
           EGPFELNDVFAI NAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK
Sbjct: 181 EGPFELNDVFAITNAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDGLVHK 240

Query: 241 RFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEINRRIK 300
           RF VGPIANVPYFRKVAAAHQLHHS+K NGVPYGLFLGPKELEEVGG EELEKEINRRIK
Sbjct: 241 RFPVGPIANVPYFRKVAAAHQLHHSEKLNGVPYGLFLGPKELEEVGGLEELEKEINRRIK 300

Query: 301 STATKSIDGS 311
           STA K I+GS
Sbjct: 301 STAKKPINGS 310

[Close]

BLAST of Tan0003703 vs. TAIR 10
Match: AT4G25700.1 (beta-hydroxylase 1 )

HSP 1 Score: 409.5 bits (1051), Expect = 2.5e-114
Identity = 215/314 (68.47%), Postives = 245/314 (78.03%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLS A+  KP H   +SS+   + R     P+S+   S       R    +VC  VE
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLR----LPKSLSGFS----PSLRFKRFSVCYVVE 60

Query: 61  DQNSSGEVEN----LADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSM 120
           ++  +  +EN     +   +  I  +  +  +AE+L RK+SER TYL+AA++SSFGITSM
Sbjct: 61  ERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGITSM 120

Query: 121 AVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESH 180
           AVMAVYYRF WQMEGGEI   EMFGTF+LSVGAAVGMEFWARWAHRALWHASLW+MHESH
Sbjct: 121 AVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMHESH 180

Query: 181 HKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHDG 240
           HKPREGPFELNDVFAI+NA PAI LLSYGFF+KGLVPGLCFGAGLGITVFG+AYMFVHDG
Sbjct: 181 HKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGAGLGITVFGIAYMFVHDG 240

Query: 241 LVHKRFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEIN 300
           LVHKRF VGPIA+VPY RKVAAAHQLHH+DKFNGVPYGLFLGPKELEEVGG EEL+KEI+
Sbjct: 241 LVHKRFPVGPIADVPYLRKVAAAHQLHHTDKFNGVPYGLFLGPKELEEVGGNEELDKEIS 300

Query: 301 RRIKSTATKSIDGS 311
           RRIKS    S  GS
Sbjct: 301 RRIKSYKKASGSGS 306

[Close]

BLAST of Tan0003703 vs. TAIR 10
Match: AT5G52570.1 (beta-carotene hydroxylase 2 )

HSP 1 Score: 396.0 bits (1016), Expect = 2.8e-110
Identity = 209/305 (68.52%), Postives = 239/305 (78.36%), Query Frame = 0


Query: 1   MAAGLSA-ALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFV 60
           MAAGLS  A+  KP +    S+N  P S    +FP S+  N       RRR +LTVC  V
Sbjct: 1   MAAGLSTIAVTLKPLNRSSFSAN-HPISTA--VFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQNSSGEVENLADEGSPIIVPQ----IPSPHVAERLARKRSERFTYLVAAVMSSFGITS 120
           E++  S  ++   D+  P         + +  + ++  +K+SERFTYL+AAVMSSFGITS
Sbjct: 61  EERKQSSPMD---DDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITS 120

Query: 121 MAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHES 180
           MA+MAVYYRF WQM+GGE+   EMFGTF+LSVGAAVGMEFWARWAHRALWH SLW+MHES
Sbjct: 121 MAIMAVYYRFSWQMKGGEVSVLEMFGTFALSVGAAVGMEFWARWAHRALWHDSLWNMHES 180

Query: 181 HHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHD 240
           HHKPREG FELNDVFAI NAVPAI LL YGF +KGLVPGLCFGAGLGIT+FGMAYMFVHD
Sbjct: 181 HHKPREGAFELNDVFAITNAVPAIGLLYYGFLNKGLVPGLCFGAGLGITMFGMAYMFVHD 240

Query: 241 GLVHKRFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEI 300
           GLVHKRF VGPIANVPY RKVAAAHQLHH+DKF GVPYGLFLGPKE+EEVGG+EELEKEI
Sbjct: 241 GLVHKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEI 294

[Close]

BLAST of Tan0003703 vs. TAIR 10
Match: AT4G25700.2 (beta-hydroxylase 1 )

HSP 1 Score: 255.8 bits (652), Expect = 4.6e-68
Identity = 138/223 (61.88%), Postives = 163/223 (73.09%), Query Frame = 0


Query: 1   MAAGLSAALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFVE 60
           MAAGLS A+  KP H   +SS+   + R     P+S+   S       R    +VC  VE
Sbjct: 1   MAAGLSTAVTFKPLHRSFSSSSTDFRLR----LPKSLSGFS----PSLRFKRFSVCYVVE 60

Query: 61  DQNSSGEVEN----LADEGSPIIVPQIPSPHVAERLARKRSERFTYLVAAVMSSFGITSM 120
           ++  +  +EN     +   +  I  +  +  +AE+L RK+SER TYL+AA++SSFGITSM
Sbjct: 61  ERRQNSPIENDERPESTSSTNAIDAEYLALRLAEKLERKKSERSTYLIAAMLSSFGITSM 120

Query: 121 AVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHESH 180
           AVMAVYYRF WQMEGGEI   EMFGTF+LSVGAAVGMEFWARWAHRALWHASLW+MHESH
Sbjct: 121 AVMAVYYRFSWQMEGGEISMLEMFGTFALSVGAAVGMEFWARWAHRALWHASLWNMHESH 180

Query: 181 HKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGA 220
           HKPREGPFELNDVFAI+NA PAI LLSYGFF+KGLVPGLCFGA
Sbjct: 181 HKPREGPFELNDVFAIVNAGPAIGLLSYGFFNKGLVPGLCFGA 215

[Close]

BLAST of Tan0003703 vs. TAIR 10
Match: AT5G52570.2 (beta-carotene hydroxylase 2 )

HSP 1 Score: 234.6 bits (597), Expect = 1.1e-61
Identity = 148/305 (48.52%), Postives = 176/305 (57.70%), Query Frame = 0


Query: 1   MAAGLSA-ALGPKPFHLFLTSSNLSPKSRTPFLFPRSVFRNSRIQWKMRRRTLLTVCVFV 60
           MAAGLS  A+  KP +    S+N  P S    +FP S+  N       RRR +LTVC  V
Sbjct: 1   MAAGLSTIAVTLKPLNRSSFSAN-HPISTA--VFPPSLRFNG-----FRRRKILTVCFVV 60

Query: 61  EDQNSSGEVENLADEGSPIIVPQ----IPSPHVAERLARKRSERFTYLVAAVMSSFGITS 120
           E++  S  ++   D+  P         + +  + ++  +K+SERFTYL+AAVMSSFGITS
Sbjct: 61  EERKQSSPMD---DDNKPESTTSSSEILMTSRLLKKAEKKKSERFTYLIAAVMSSFGITS 120

Query: 121 MAVMAVYYRFYWQMEGGEIPFSEMFGTFSLSVGAAVGMEFWARWAHRALWHASLWHMHES 180
           MA+MAVYYRF WQM+GGE+   EMFGTF+LSVGA                          
Sbjct: 121 MAIMAVYYRFSWQMKGGEVSVLEMFGTFALSVGA-------------------------- 180

Query: 181 HHKPREGPFELNDVFAIINAVPAIALLSYGFFHKGLVPGLCFGAGLGITVFGMAYMFVHD 240
                                                      AGLGIT+FGMAYMFVHD
Sbjct: 181 -------------------------------------------AGLGITMFGMAYMFVHD 225

Query: 241 GLVHKRFLVGPIANVPYFRKVAAAHQLHHSDKFNGVPYGLFLGPKELEEVGGQEELEKEI 300
           GLVHKRF VGPIANVPY RKVAAAHQLHH+DKF GVPYGLFLGPKE+EEVGG+EELEKEI
Sbjct: 241 GLVHKRFPVGPIANVPYLRKVAAAHQLHHTDKFKGVPYGLFLGPKEVEEVGGKEELEKEI 225

The following BLAST results are available for this feature:

BLAST of Tan0003703 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O49814	5.9e-121	75.96	Beta-carotene hydroxylase 2, chloroplastic OS=Capsicum annuum OX=4072 GN=CA2 PE=...	[more]
B3SGL0	1.7e-115	71.72	Beta-carotene 3-hydroxylase, chloroplastic OS=Gentiana lutea OX=38851 GN=BHY PE=...	[more]
Q9SZZ8	3.5e-113	68.47	Beta-carotene 3-hydroxylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]
O49815	2.8e-110	71.53	Beta-carotene hydroxylase 1, chloroplastic OS=Capsicum annuum OX=4072 GN=CA1 PE=...	[more]
Q9LTG0	4.0e-109	68.52	Beta-carotene 3-hydroxylase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=...	[more]

BLAST of Tan0003703 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038877448.1	4.2e-164	93.87	beta-carotene hydroxylase 2, chloroplastic [Benincasa hispida]	[more]
KAG7013258.1	9.3e-164	92.90	Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma su...	[more]
XP_022944865.1	2.1e-163	92.58	beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita moschata]	[more]
KAG6574199.1	7.9e-163	92.58	Beta-carotene 3-hydroxylase 1, chloroplastic, partial [Cucurbita argyrosperma su...	[more]
XP_023541483.1	1.3e-162	91.94	beta-carotene hydroxylase 2, chloroplastic-like isoform X1 [Cucurbita pepo subsp...	[more]

BLAST of Tan0003703 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FZ94	1.0e-163	92.58	beta-carotene hydroxylase 2, chloroplastic-like isoform X1 OS=Cucurbita moschata...	[more]
A0A0A0L606	1.1e-162	91.94	Fatty acid hydroxylase domain-containing protein OS=Cucumis sativus OX=3659 GN=C...	[more]
A0A6J1E4L5	2.5e-162	91.94	beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1J1S8	5.5e-162	92.26	beta-carotene hydroxylase 2, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1HX82	2.1e-161	91.94	beta-carotene hydroxylase 2, chloroplastic-like isoform X2 OS=Cucurbita maxima O...	[more]

BLAST of Tan0003703 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G25700.1	2.5e-114	68.47	beta-hydroxylase 1	[more]
AT5G52570.1	2.8e-110	68.52	beta-carotene hydroxylase 2	[more]
AT4G25700.2	4.6e-68	61.88	beta-hydroxylase 1	[more]
AT5G52570.2	1.1e-61	48.52	beta-carotene hydroxylase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR045019	Beta-carotene hydroxylase-like	PANTHER	PTHR31899	BETA-CAROTENE 3-HYDROXYLASE 1, CHLOROPLASTIC	coord: 1..220

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0003703.1	Tan0003703.1	mRNA
Tan0003703.2	Tan0003703.2	mRNA
Tan0003703.3	Tan0003703.3	mRNA
Tan0003703.4	Tan0003703.4	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008610	lipid biosynthetic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0016491	oxidoreductase activity