Tan0003654 (gene) Snake gourd v1

BLAST of Tan0003654 vs. ExPASy Swiss-Prot
Match: Q54I39 (IST1-like protein OS=Dictyostelium discoideum OX=44689 GN=DDB_G0289029 PE=3 SV=1)

HSP 1 Score: 80.5 bits (197), Expect = 5.6e-14
Identity = 47/180 (26.11%), Postives = 97/180 (53.89%), Query Frame = 0

Query: 1   MFGMFLKPKYYT-KCKSCVKLTKARLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRA 60
           MFG    P Y + K K  +KL  +R+  ++ KK  +++  K ++ ELL+ + + +A  R 
Sbjct: 1   MFG--FGPSYDSYKLKVQLKLAVSRIQILKNKKANIVRDEKRNVAELLRKKNEESARIRV 60

Query: 61  EGFLVERNVLRCYELIDEFCGIIFNQLSVLNKQSECPDECKEAVASLIYAAARFADLPEL 120
           E  + +  ++ C+++I+  C ++  +++++N  +E P E KE++ +L+Y++ R   +PEL
Sbjct: 61  ETIIRDEYLIECFQIIEVLCELLHARINLINATTEMPLEMKESIFTLVYSSQRI-QIPEL 120

Query: 121 RELRNLFTGKYGSSFE--------SFTNKELIEKSNTIALTKEMKLQLLQEIVQESAIDW 172
            +++N    KYG   E        +  N +++ K +       +  Q L EI ++  +DW
Sbjct: 121 EQIKNQLKAKYGKGLENEANCHCSTHVNPKIVHKLSYATPDPSIIFQTLSEIAEKFNVDW 177

BLAST of Tan0003654 vs. NCBI nr
Match: XP_038897244.1 (uncharacterized protein LOC120085370 [Benincasa hispida])

HSP 1 Score: 674.9 bits (1740), Expect = 5.2e-190
Identity = 369/472 (78.18%), Postives = 411/472 (87.08%), Query Frame = 0

Query: 1   MFGMFLKPKYYTKCKSCVKLTKARLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF MFLKPK+YTKCKSCVK+TK RLD+IRKKK AVLKYLK+DIVELLKSRLDYNAYNRAE
Sbjct: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDSIRKKKKAVLKYLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGIIFNQLSVLNKQSECPDECKEAVASLIYAAARFADLPELR 120
           GFLVERNVLRCY LIDEFCG I +Q+ VL+K+SECP+ECKEAVA+LIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYLLIDEFCGTISSQIPVLSKESECPEECKEAVATLIYAAARFADLPELR 120

Query: 121 ELRNLFTGKYGSSFESFTNKELIEKSNTIALTKEMKLQLLQEIVQESAIDWNSKALEQQL 180
           ELR+LFT KYGSSF SFTNKELIEKS   A TKEMK+QLLQEI Q+SAIDWNSKALEQQL
Sbjct: 121 ELRSLFTEKYGSSFGSFTNKELIEKSRATAQTKEMKIQLLQEIAQDSAIDWNSKALEQQL 180

Query: 181 YT-PPQNEPDCDRRSTEKRNKTKVAFVPVHERKKSSLEKKDNSDDDSMFDSRSEGNTTET 240
           YT PPQNEP  +R +T KRNKTK+  VPVHERK  S  KKDNSD++S+FDSRSEGNTTET
Sbjct: 181 YTPPPQNEPAGERSATIKRNKTKIVTVPVHERKTISPRKKDNSDNESIFDSRSEGNTTET 240

Query: 241 STGDSSTDQDVRKGVSEDEVEDQKPFYLRVIRPPYLKTKPTNKE-TMEEARKATDHIEND 300
           STGD STDQDV KG+SEDEVEDQKPFYLR + PPYLKTKP   E  +EE++K T    ND
Sbjct: 241 STGD-STDQDVHKGISEDEVEDQKPFYLRFVPPPYLKTKPIKTEANVEESQKVTVG-NND 300

Query: 301 ESNKQQPKPTSEEKPKPRSVRRRNVKPQPARDINIDDVGNSANDGVEKSSLSRNKGKETM 360
           ESNKQ PK  +EEKPKPRSVRRRNVKPQPARDINIDD G+S  DG EK+SLS+NKGKETM
Sbjct: 301 ESNKQHPKSPTEEKPKPRSVRRRNVKPQPARDINIDDAGSSTIDGAEKTSLSQNKGKETM 360

Query: 361 IGEEKGAGDDEGKMLDGLLMHYSKKK-SHESNKAKGNLKFQRQQEKDNVEPQKPKNTKTE 420
           IGEE+GAGDDE ++LDGLLM YS+KK S ESN+ KGNLK QRQQEKDN EPQKPK+TKTE
Sbjct: 361 IGEERGAGDDEERVLDGLLMQYSRKKTSQESNRGKGNLKPQRQQEKDNAEPQKPKSTKTE 420

Query: 421 DAFVSTKAVSVPIDSTEPTEPVKKHTRSNSFVHPKLPDYDQLAARFTALKEE 470
           D+FV  +AVS+P   T+ +EPVKKHTR+NSFVHPKLP+YDQLAAR  ALKE+
Sbjct: 421 DSFVPARAVSLP---TDQSEPVKKHTRTNSFVHPKLPEYDQLAARIAALKEK 467

BLAST of Tan0003654 vs. NCBI nr
Match: XP_008462153.1 (PREDICTED: uncharacterized protein LOC103500577 [Cucumis melo])

HSP 1 Score: 628.6 bits (1620), Expect = 4.2e-176
Identity = 354/473 (74.84%), Postives = 391/473 (82.66%), Query Frame = 0

Query: 1   MFGMFLKPKYYTKCKSCVKLTKARLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF MFLKPK+YTKCKSCVK+TK RLD IRKKKNAVLKYLK+DIVELLKSRLDYNA+NRAE
Sbjct: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGIIFNQLSVLNKQSECPDECKEAVASLIYAAARFADLPELR 120
           GFLVERNVLRCYELIDEFCG I NQ+ VLNK+SECPDECKEAVA+LIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELR 120

Query: 121 ELRNLFTGKYGSSFESFTNKELIEKSNTIALTKEMKLQLLQEIVQESAIDWNSKALEQQL 180
           ELRNLFT KYGSSF SFTNKE IEKS     TKEMK+QLLQEI QE+AIDWNSKALEQQL
Sbjct: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180

Query: 181 YT-PPQNEPDCDRRSTEKRNKTKVAFVPVHERKKSSLEKKDNSDDDSMFDSRSEGNTTET 240
           YT PPQNE D +R    KRNKTKV  VPV+ERK +S   K+NSD++S+FDSRSEGNTTET
Sbjct: 181 YTPPPQNELDGERSGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTET 240

Query: 241 STGDSSTDQDVRKGVSEDEVEDQKPFYLRVIRPPYLKTKPTNKETME-EARKATDHIEND 300
           STGD STDQD+ KGVSEDEVEDQKPF  R ++PPYLKTKP   E  E E RK T  IEND
Sbjct: 241 STGD-STDQDIHKGVSEDEVEDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVT--IEND 300

Query: 301 ESNKQQPKPTSEEKPKPRSVRRRNVKPQPARDINIDDVGNSANDGVEKSSLSRNKGKETM 360
           ESN   PK  +EEKPKPRSVRRRNVKPQPARDINIDDVG+S  D   K S SRNKGKETM
Sbjct: 301 ESN--NPKSPTEEKPKPRSVRRRNVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETM 360

Query: 361 IGEEKGAGDDEGKMLDGLLMHYSKKKSHESNKAKG--NLKFQRQQEKDNVEPQKPKNTKT 420
           IGEEKGA DDE ++LDGLLM YSKKK+++ +K++G  NLK QRQQEKDN+E Q+P     
Sbjct: 361 IGEEKGARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRP----- 420

Query: 421 EDAFVSTKAVSVPIDSTEPTEPVKKHTRSNSFVHPKLPDYDQLAARFTALKEE 470
                +T+AVS+P D  E T   KKHTR+NSFVHPKLP+YDQLAAR  ALKE+
Sbjct: 421 -----TTRAVSLPTDQNEST---KKHTRTNSFVHPKLPEYDQLAARIAALKEK 455

BLAST of Tan0003654 vs. NCBI nr
Match: KAG6575541.1 (IST1-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 626.3 bits (1614), Expect = 2.1e-175
Identity = 340/471 (72.19%), Postives = 380/471 (80.68%), Query Frame = 0

Query: 1   MFGMFLKPKYYTKCKSCVKLTKARLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF MF KPK+YTKCKSCVK+TK RLD IRKKK  VLK+LK+DIVELLKSRLDYNAYNRAE
Sbjct: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGIIFNQLSVLNKQSECPDECKEAVASLIYAAARFADLPELR 120
           GFLVE+NVLRCY+LIDEFCG IF  +SVLNK+SECPDECKEAVASLIYAAARF+DLPELR
Sbjct: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120

Query: 121 ELRNLFTGKYGSSFESFTNKELIEKSNTIALTKEMKLQLLQEIVQESAIDWNSKALEQQL 180
            LR+LFTG+YG+SF SFTNKEL+EK   +A TKE KLQLLQEI QESAIDWNSKALEQQL
Sbjct: 121 VLRSLFTGRYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YTPPQNEPDCDRRSTEKRNKTKVAFVPVHERKKSSLEKKDNSDDDSMFDSRSEGNTTETS 240
           Y PPQNE D +R ++  RNK+K+  VPV+ R  +S  KK+NSDDDS+FDSRSEGNTTETS
Sbjct: 181 YVPPQNELDRERSTSTNRNKSKIVSVPVYGRTTNSPRKKENSDDDSIFDSRSEGNTTETS 240

Query: 241 TGDSSTDQDVRKG-VSEDEVEDQKPFYLRVIRPPYLKTKPTNK-ETMEEARKATDHIEND 300
            GDSSTDQDV KG VSEDEVEDQKPFYLR I PPYLKTKPT K E +EE +  T      
Sbjct: 241 MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVT------ 300

Query: 301 ESNKQQPKPTSEEKPKPRSVRRRNVKPQPARDINIDDVGNSANDGVEKSSLSRNKGKETM 360
           E N+  PK   EEKPKPRSVRRR+ KPQPAR INIDD G SA DG  K S  RNKGKE M
Sbjct: 301 EENRNDPKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSGRNKGKEAM 360

Query: 361 IGEEKGAGDDEGKMLDGLLMHYSKKKSHESNKAKGNLKFQRQQEKDNVEPQKPKNTKTED 420
            GEEKG  DDE +MLDGLL  YSKKK+             RQ++KDN EPQ+PKNT+TED
Sbjct: 361 TGEEKGVEDDEERMLDGLLKLYSKKKT-------------RQEDKDNAEPQRPKNTRTED 420

Query: 421 AFVSTKAVSVPIDSTEPTEPVKKHTRSNSFVHPKLPDYDQLAARFTALKEE 470
           A+V TK++S+P   TEP EPVKKH R+NS+VHPKLPDYDQLAARF ALKE+
Sbjct: 421 AYVPTKSLSLPPPRTEPIEPVKKHARANSYVHPKLPDYDQLAARFAALKEK 452

BLAST of Tan0003654 vs. NCBI nr
Match: XP_022953212.1 (uncharacterized protein LOC111455825 [Cucurbita moschata])

HSP 1 Score: 625.9 bits (1613), Expect = 2.7e-175
Identity = 340/471 (72.19%), Postives = 381/471 (80.89%), Query Frame = 0

Query: 1   MFGMFLKPKYYTKCKSCVKLTKARLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF MF KPK+YTKCKSCVK+TK RLD IRKKK  VLK+LK+DIVELLKSRLDYNAYNRAE
Sbjct: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGIIFNQLSVLNKQSECPDECKEAVASLIYAAARFADLPELR 120
           GFLVE+NVLRCY+LIDEFCG IF  +SVLNK+SECPDECKEAVASLIYAAARF+DLPELR
Sbjct: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120

Query: 121 ELRNLFTGKYGSSFESFTNKELIEKSNTIALTKEMKLQLLQEIVQESAIDWNSKALEQQL 180
            LR+LFT +YG+SF SFTNKEL+EK   +A TKE KLQLLQEI QESAIDWNSKALEQQL
Sbjct: 121 VLRSLFTERYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YTPPQNEPDCDRRSTEKRNKTKVAFVPVHERKKSSLEKKDNSDDDSMFDSRSEGNTTETS 240
           Y PPQNE DC+R ++  R+K+K+  VPV+ R  +S  KKDNSDDDS+FDSRSEGNTTETS
Sbjct: 181 YVPPQNELDCERSTSTCRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETS 240

Query: 241 TGDSSTDQDVRKG-VSEDEVEDQKPFYLRVIRPPYLKTKPTNK-ETMEEARKATDHIEND 300
            GDSSTDQDV KG VSEDEVEDQKPFYLR I PPYLKTKPT K E +EE +  T      
Sbjct: 241 MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNDT------ 300

Query: 301 ESNKQQPKPTSEEKPKPRSVRRRNVKPQPARDINIDDVGNSANDGVEKSSLSRNKGKETM 360
           E N+  PK   EEKPKPRSVRRR+ KPQPAR INIDD G SA DG  K S SRNKGKE M
Sbjct: 301 EENRNDPKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSSRNKGKEAM 360

Query: 361 IGEEKGAGDDEGKMLDGLLMHYSKKKSHESNKAKGNLKFQRQQEKDNVEPQKPKNTKTED 420
            GEEKG  DDE +MLDGLL  YSKKK+             RQ++KDN EPQ+PKNT+TED
Sbjct: 361 TGEEKGVEDDEERMLDGLLKLYSKKKT-------------RQEDKDNAEPQRPKNTRTED 420

Query: 421 AFVSTKAVSVPIDSTEPTEPVKKHTRSNSFVHPKLPDYDQLAARFTALKEE 470
           A+V TK++S+P   TEP EPVKKH R+NS+VHPKLPDYDQLAARF +LKE+
Sbjct: 421 AYVPTKSLSLPPPRTEPIEPVKKHARANSYVHPKLPDYDQLAARFASLKEK 452

BLAST of Tan0003654 vs. NCBI nr
Match: KAG7014085.1 (IST1-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 625.9 bits (1613), Expect = 2.7e-175
Identity = 340/471 (72.19%), Postives = 380/471 (80.68%), Query Frame = 0

Query: 1   MFGMFLKPKYYTKCKSCVKLTKARLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF MF KPK+YTKCKSCVK+TK RLD IRKKK  VLK+LK+DIVELLKSRLDYNAYNRAE
Sbjct: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGIIFNQLSVLNKQSECPDECKEAVASLIYAAARFADLPELR 120
           GFLVE+NVLRCY+LIDEFCG IF  +SVLNK+SECPDECKEAVASLIYAAARF+DLPELR
Sbjct: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120

Query: 121 ELRNLFTGKYGSSFESFTNKELIEKSNTIALTKEMKLQLLQEIVQESAIDWNSKALEQQL 180
            LR+LFTG+YG+SF SFTNKEL+EK   +A TKE KLQLLQEI QESAIDWNSKALEQQL
Sbjct: 121 VLRSLFTGRYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YTPPQNEPDCDRRSTEKRNKTKVAFVPVHERKKSSLEKKDNSDDDSMFDSRSEGNTTETS 240
           Y PPQNE D +R ++  R+K+K+  VPV+ R  +S  KKDNSDDDS+FDSRSEGNTTETS
Sbjct: 181 YVPPQNELDRERSTSTNRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETS 240

Query: 241 TGDSSTDQDVRKG-VSEDEVEDQKPFYLRVIRPPYLKTKPTNK-ETMEEARKATDHIEND 300
            GDSSTDQDV KG VSEDEVEDQKPFYLR I PPYLKTKPT K E +EE +  T      
Sbjct: 241 MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVT------ 300

Query: 301 ESNKQQPKPTSEEKPKPRSVRRRNVKPQPARDINIDDVGNSANDGVEKSSLSRNKGKETM 360
           E N+  PK   EEKPKPRSVRRR+ KPQPAR INIDD G SA DG  K S  RNKGKE M
Sbjct: 301 EENRNDPKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSGRNKGKEAM 360

Query: 361 IGEEKGAGDDEGKMLDGLLMHYSKKKSHESNKAKGNLKFQRQQEKDNVEPQKPKNTKTED 420
            GEEKG  DDE +MLDGLL  YSKKK+             RQ++KDN EPQ+PKNT+TED
Sbjct: 361 TGEEKGVEDDEERMLDGLLKLYSKKKT-------------RQEDKDNAEPQRPKNTRTED 420

Query: 421 AFVSTKAVSVPIDSTEPTEPVKKHTRSNSFVHPKLPDYDQLAARFTALKEE 470
           A+V TK++S+P   TEP EPVKKH R+NS+VHPKLPDYDQLAARF ALKE+
Sbjct: 421 AYVPTKSLSLPPPRTEPIEPVKKHARANSYVHPKLPDYDQLAARFAALKEK 452

BLAST of Tan0003654 vs. ExPASy TrEMBL
Match: A0A1S3CGT5 (uncharacterized protein LOC103500577 OS=Cucumis melo OX=3656 GN=LOC103500577 PE=3 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 2.1e-176
Identity = 354/473 (74.84%), Postives = 391/473 (82.66%), Query Frame = 0

Query: 1   MFGMFLKPKYYTKCKSCVKLTKARLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF MFLKPK+YTKCKSCVK+TK RLD IRKKKNAVLKYLK+DIVELLKSRLDYNA+NRAE
Sbjct: 1   MFDMFLKPKFYTKCKSCVKMTKTRLDTIRKKKNAVLKYLKNDIVELLKSRLDYNAFNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGIIFNQLSVLNKQSECPDECKEAVASLIYAAARFADLPELR 120
           GFLVERNVLRCYELIDEFCG I NQ+ VLNK+SECPDECKEAVA+LIYAAARFADLPELR
Sbjct: 61  GFLVERNVLRCYELIDEFCGTISNQIPVLNKESECPDECKEAVATLIYAAARFADLPELR 120

Query: 121 ELRNLFTGKYGSSFESFTNKELIEKSNTIALTKEMKLQLLQEIVQESAIDWNSKALEQQL 180
           ELRNLFT KYGSSF SFTNKE IEKS     TKEMK+QLLQEI QE+AIDWNSKALEQQL
Sbjct: 121 ELRNLFTEKYGSSFGSFTNKEFIEKSRATTQTKEMKIQLLQEIAQETAIDWNSKALEQQL 180

Query: 181 YT-PPQNEPDCDRRSTEKRNKTKVAFVPVHERKKSSLEKKDNSDDDSMFDSRSEGNTTET 240
           YT PPQNE D +R    KRNKTKV  VPV+ERK +S   K+NSD++S+FDSRSEGNTTET
Sbjct: 181 YTPPPQNELDGERSGATKRNKTKVVSVPVYERKANSPRNKNNSDNESIFDSRSEGNTTET 240

Query: 241 STGDSSTDQDVRKGVSEDEVEDQKPFYLRVIRPPYLKTKPTNKETME-EARKATDHIEND 300
           STGD STDQD+ KGVSEDEVEDQKPF  R ++PPYLKTKP   E  E E RK T  IEND
Sbjct: 241 STGD-STDQDIHKGVSEDEVEDQKPFNHRFVQPPYLKTKPIKTEANEKEPRKVT--IEND 300

Query: 301 ESNKQQPKPTSEEKPKPRSVRRRNVKPQPARDINIDDVGNSANDGVEKSSLSRNKGKETM 360
           ESN   PK  +EEKPKPRSVRRRNVKPQPARDINIDDVG+S  D   K S SRNKGKETM
Sbjct: 301 ESN--NPKSPTEEKPKPRSVRRRNVKPQPARDINIDDVGSSTIDVTTKISSSRNKGKETM 360

Query: 361 IGEEKGAGDDEGKMLDGLLMHYSKKKSHESNKAKG--NLKFQRQQEKDNVEPQKPKNTKT 420
           IGEEKGA DDE ++LDGLLM YSKKK+++ +K++G  NLK QRQQEKDN+E Q+P     
Sbjct: 361 IGEEKGARDDEERVLDGLLMQYSKKKTNQESKSRGKSNLKPQRQQEKDNIEHQRP----- 420

Query: 421 EDAFVSTKAVSVPIDSTEPTEPVKKHTRSNSFVHPKLPDYDQLAARFTALKEE 470
                +T+AVS+P D  E T   KKHTR+NSFVHPKLP+YDQLAAR  ALKE+
Sbjct: 421 -----TTRAVSLPTDQNEST---KKHTRTNSFVHPKLPEYDQLAARIAALKEK 455

BLAST of Tan0003654 vs. ExPASy TrEMBL
Match: A0A6J1GMM1 (uncharacterized protein LOC111455825 OS=Cucurbita moschata OX=3662 GN=LOC111455825 PE=3 SV=1)

HSP 1 Score: 625.9 bits (1613), Expect = 1.3e-175
Identity = 340/471 (72.19%), Postives = 381/471 (80.89%), Query Frame = 0

Query: 1   MFGMFLKPKYYTKCKSCVKLTKARLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF MF KPK+YTKCKSCVK+TK RLD IRKKK  VLK+LK+DIVELLKSRLDYNAYNRAE
Sbjct: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGIIFNQLSVLNKQSECPDECKEAVASLIYAAARFADLPELR 120
           GFLVE+NVLRCY+LIDEFCG IF  +SVLNK+SECPDECKEAVASLIYAAARF+DLPELR
Sbjct: 61  GFLVEQNVLRCYDLIDEFCGTIFKHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120

Query: 121 ELRNLFTGKYGSSFESFTNKELIEKSNTIALTKEMKLQLLQEIVQESAIDWNSKALEQQL 180
            LR+LFT +YG+SF SFTNKEL+EK   +A TKE KLQLLQEI QESAIDWNSKALEQQL
Sbjct: 121 VLRSLFTERYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YTPPQNEPDCDRRSTEKRNKTKVAFVPVHERKKSSLEKKDNSDDDSMFDSRSEGNTTETS 240
           Y PPQNE DC+R ++  R+K+K+  VPV+ R  +S  KKDNSDDDS+FDSRSEGNTTETS
Sbjct: 181 YVPPQNELDCERSTSTCRSKSKIVSVPVYGRTTNSPRKKDNSDDDSIFDSRSEGNTTETS 240

Query: 241 TGDSSTDQDVRKG-VSEDEVEDQKPFYLRVIRPPYLKTKPTNK-ETMEEARKATDHIEND 300
            GDSSTDQDV KG VSEDEVEDQKPFYLR I PPYLKTKPT K E +EE +  T      
Sbjct: 241 MGDSSTDQDVHKGVVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNDT------ 300

Query: 301 ESNKQQPKPTSEEKPKPRSVRRRNVKPQPARDINIDDVGNSANDGVEKSSLSRNKGKETM 360
           E N+  PK   EEKPKPRSVRRR+ KPQPAR INIDD G SA DG  K S SRNKGKE M
Sbjct: 301 EENRNDPKTAIEEKPKPRSVRRRHAKPQPARSINIDDAGGSAADGAGKISSSRNKGKEAM 360

Query: 361 IGEEKGAGDDEGKMLDGLLMHYSKKKSHESNKAKGNLKFQRQQEKDNVEPQKPKNTKTED 420
            GEEKG  DDE +MLDGLL  YSKKK+             RQ++KDN EPQ+PKNT+TED
Sbjct: 361 TGEEKGVEDDEERMLDGLLKLYSKKKT-------------RQEDKDNAEPQRPKNTRTED 420

Query: 421 AFVSTKAVSVPIDSTEPTEPVKKHTRSNSFVHPKLPDYDQLAARFTALKEE 470
           A+V TK++S+P   TEP EPVKKH R+NS+VHPKLPDYDQLAARF +LKE+
Sbjct: 421 AYVPTKSLSLPPPRTEPIEPVKKHARANSYVHPKLPDYDQLAARFASLKEK 452

BLAST of Tan0003654 vs. ExPASy TrEMBL
Match: A0A6J1JV69 (uncharacterized protein LOC111488629 OS=Cucurbita maxima OX=3661 GN=LOC111488629 PE=3 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 8.1e-173
Identity = 336/471 (71.34%), Postives = 379/471 (80.47%), Query Frame = 0

Query: 1   MFGMFLKPKYYTKCKSCVKLTKARLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF MF KPK+YTKCKSCVK+TK RLD IRKKK  VLK+LK+DIVELLKSRLDYNAYNRAE
Sbjct: 1   MFRMFFKPKFYTKCKSCVKMTKTRLDTIRKKKKVVLKFLKNDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGIIFNQLSVLNKQSECPDECKEAVASLIYAAARFADLPELR 120
           GFLVE+NVLRCY+LIDEFCG IFN +SVLNK+SECPDECKEAVASLIYAAARF+DLPELR
Sbjct: 61  GFLVEQNVLRCYDLIDEFCGTIFNHISVLNKESECPDECKEAVASLIYAAARFSDLPELR 120

Query: 121 ELRNLFTGKYGSSFESFTNKELIEKSNTIALTKEMKLQLLQEIVQESAIDWNSKALEQQL 180
            LR+LFT +YG+SF SFTNKEL+EK   +A TKE KLQLLQEI QESAIDWNSKALEQQL
Sbjct: 121 VLRSLFTERYGTSFGSFTNKELVEKLRAVAQTKETKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YTPPQNEPDCDRRSTEKRNKTKVAFVPVHERKKSSLEKKDNSDDDSMFDSRSEGNTTETS 240
           Y PPQNE   +R ++  R+K+K+  VPV+ R  +S +KKDNSDDDS+FDSRSEGNTTETS
Sbjct: 181 YVPPQNELVRERSTSTNRSKSKIVSVPVYGRTTNSPKKKDNSDDDSIFDSRSEGNTTETS 240

Query: 241 TGDSSTDQDVRKG-VSEDEVEDQKPFYLRVIRPPYLKTKPTNK-ETMEEARKATDHIEND 300
            GDSSTDQDV KG VSEDEVEDQKPFYLR I PPYLKTKPT K E +EE +  T      
Sbjct: 241 MGDSSTDQDVHKGIVSEDEVEDQKPFYLRFITPPYLKTKPTKKEENVEEPQNVT------ 300

Query: 301 ESNKQQPKPTSEEKPKPRSVRRRNVKPQPARDINIDDVGNSANDGVEKSSLSRNKGKETM 360
           E  +  PK T+EEKPKPRSVRRR+ KPQPAR INIDD G SA DG  K S  +NKGKE M
Sbjct: 301 EEKRNNPKTTTEEKPKPRSVRRRHAKPQPARSINIDDSGGSAADGAGKISSGQNKGKEAM 360

Query: 361 IGEEKGAGDDEGKMLDGLLMHYSKKKSHESNKAKGNLKFQRQQEKDNVEPQKPKNTKTED 420
            GEEKG  DDE +MLDGLL  YSKK +             RQ++KDN EPQ+PKNT+TED
Sbjct: 361 TGEEKGEEDDEERMLDGLLKIYSKKNT-------------RQEDKDNAEPQRPKNTRTED 420

Query: 421 AFVSTKAVSVPIDSTEPTEPVKKHTRSNSFVHPKLPDYDQLAARFTALKEE 470
           A+V  K++S+P   TEP EPVKKH R+NS+VHPKLPDYDQLAARF ALKE+
Sbjct: 421 AYVPAKSLSLPTARTEPIEPVKKHARANSYVHPKLPDYDQLAARFAALKEK 452

BLAST of Tan0003654 vs. ExPASy TrEMBL
Match: A0A6J1CWS8 (vacuolar protein sorting-associated protein IST1 OS=Momordica charantia OX=3673 GN=LOC111015509 PE=3 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 3.1e-172
Identity = 339/471 (71.97%), Postives = 387/471 (82.17%), Query Frame = 0

Query: 1   MFGMFLKPKYYTKCKSCVKLTKARLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF MF KPK++TKCKSCVKL K RLD IRKKKNAVLK+LK+DI ELL+S LD NAYNRAE
Sbjct: 1   MFDMFFKPKFFTKCKSCVKLLKMRLDTIRKKKNAVLKFLKNDIAELLRSGLDQNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGIIFNQLSVLNKQSECPDECKEAVASLIYAAARFADLPELR 120
           G LVERNVL+CY+LIDEF G I+NQLSVL+KQSECPDEC+EAVASLIYAAARFADLPELR
Sbjct: 61  GLLVERNVLKCYDLIDEFSGTIYNQLSVLSKQSECPDECREAVASLIYAAARFADLPELR 120

Query: 121 ELRNLFTGKYGSSFESFTNKELIEKSNTIALTKEMKLQLLQEIVQESAIDWNSKALEQQL 180
           ELR+LFT KYG SFE FTNKELIEK + +A+TKE+KLQLLQEI QES I+WNSKALEQQL
Sbjct: 121 ELRSLFTAKYGRSFEQFTNKELIEKPSAMAMTKEVKLQLLQEIAQESGIEWNSKALEQQL 180

Query: 181 YTPPQNEPDCDRRSTEKRNKTKVAFVPVHERKKSSLEKKDNSDDDSMFDSRSEGNTTETS 240
           YTPPQN PD +  + ++RNK KV  +PV ERK S L+KKDNSDDDS+FDSRSE N TETS
Sbjct: 181 YTPPQNTPD-EEPNADRRNKAKVVSIPVFERKTSLLQKKDNSDDDSIFDSRSEDNGTETS 240

Query: 241 TGDSSTDQDVRKGVSEDEVEDQKPFYLRVIRPPYLKTKPTNKETMEEARKATDHIENDES 300
           TGDSSTDQDV+KGV EDEVED+KPFYLR I PPYLK+KP+ KE   E             
Sbjct: 241 TGDSSTDQDVQKGVFEDEVEDRKPFYLRFIPPPYLKSKPSKKEASFE------------- 300

Query: 301 NKQQPKPTSEEKPKPRSVRRRNVKPQPARDINIDDVGNSANDGVEKSSLSRNKGKETMIG 360
                KP  EEKPKPRSVRRRN K +P RDINI +V NSANDG EK+S SRNKGKETM+G
Sbjct: 301 -----KPAVEEKPKPRSVRRRNSKLEPTRDINIGNVDNSANDGTEKASSSRNKGKETMMG 360

Query: 361 EEKGAGDDEGKMLDGLLMHYSKKKS-HESNKAKGNLKFQRQQEKDNVEPQKPKNTKT-ED 420
           EEKGAG++E +MLDGLLMHYSKKKS  ESN+AKGNLK Q+ +E+D  EPQ+ KN+K  ED
Sbjct: 361 EEKGAGEEE-RMLDGLLMHYSKKKSTQESNRAKGNLKSQKHKEQD--EPQRTKNSKAEED 420

Query: 421 AFVSTKAVSVPIDSTEPTEPVKKHTRSNSFVHPKLPDYDQLAARFTALKEE 470
             V  ++VS+P D+ EP  P+K+HTR+NSFVHPKLPDYDQLAARF ALKE+
Sbjct: 421 LSVPGRSVSLPRDTNEPIVPMKRHTRNNSFVHPKLPDYDQLAARFAALKEK 449

BLAST of Tan0003654 vs. ExPASy TrEMBL
Match: A0A6J1KJN9 (uncharacterized protein LOC111494616 OS=Cucurbita maxima OX=3661 GN=LOC111494616 PE=3 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 9.9e-171
Identity = 348/474 (73.42%), Postives = 371/474 (78.27%), Query Frame = 0

Query: 1   MFGMFLKPKYYTKCKSCVKLTKARLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MFGMFLKPKYYTKCKSC+KL K+RLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE
Sbjct: 1   MFGMFLKPKYYTKCKSCLKLLKSRLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGIIFNQLSVLNKQSECPDECKEAVASLIYAAARFADLPELR 120
           GFLVERNVL+CYELIDEFCG I NQLSVL KQSECPDE KEAVASLIYAAARFADLPELR
Sbjct: 61  GFLVERNVLKCYELIDEFCGAISNQLSVLIKQSECPDEFKEAVASLIYAAARFADLPELR 120

Query: 121 ELRNLFTGKYGSSFESFTNKELIEKSNTIALTKEMKLQLLQEIVQESAIDWNSKALEQQL 180
           ELRN FTGKYG SFE FT+ ELIEKS     TKE+KLQLLQEI QESAIDWNSKALEQQL
Sbjct: 121 ELRNFFTGKYGCSFEPFTSIELIEKSRATIQTKEIKLQLLQEIAQESAIDWNSKALEQQL 180

Query: 181 YTPPQNEPDCDRRSTEKRNKTKVAFVPVHERKKSSLEKKDNSDDDSMFDSRSEGNTTETS 240
           Y PP NEPD                VP+HERKK S+ K+D+SDD+SMFD RSEGNTTETS
Sbjct: 181 YIPPPNEPD---------------VVPMHERKKDSVGKRDSSDDESMFDIRSEGNTTETS 240

Query: 241 TGDSSTDQDVRKGVSEDEVEDQKPFYLRVIRPPYLKTKPTNKETMEEARKATDHIENDES 300
           T DSSTDQDVRKG SEDEVEDQ PFYLRVIRPPYLKTKPTNKETMEEARKATD +ENDE 
Sbjct: 241 TVDSSTDQDVRKGGSEDEVEDQTPFYLRVIRPPYLKTKPTNKETMEEARKATDQVENDEC 300

Query: 301 NKQQPKPTSEE--KPKPRSVRRRNVKPQPARDINIDDVGNSANDGVEKSSLSRNKGKETM 360
            KQQP+PTSEE  KPKP+SVRRRNVK QP  +I IDDVGNS ND     S SRNKGKE M
Sbjct: 301 KKQQPEPTSEEKPKPKPKSVRRRNVKLQPTMEIKIDDVGNSGND----MSSSRNKGKEAM 360

Query: 361 IGEEKGAGDDEGKMLDGLLMHYSKKK-SHESNKAKGNLKFQRQQEKDNVEPQKPKNTKTE 420
            GE     ++E KMLDGLLMHYSKKK SHESN+                           
Sbjct: 361 KGEGDDEEEEEEKMLDGLLMHYSKKKPSHESNE--------------------------- 420

Query: 421 DAFVSTKAVSVPIDSTEPTE-PVKKHTRSNSFVHPKLPDYDQLAARFTALKEEK 471
                 +AVS+ IDSTEPTE   K+HTR+NSFVHP LPDYD+LAARF ALKE+K
Sbjct: 421 -----VRAVSLSIDSTEPTELENKRHTRTNSFVHPNLPDYDELAARFAALKEDK 423

BLAST of Tan0003654 vs. TAIR 10
Match: AT1G79910.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 240.7 bits (613), Expect = 2.3e-63
Identity = 156/370 (42.16%), Postives = 227/370 (61.35%), Query Frame = 0

Query: 1   MFGMFLKPKYYTKCKSCVKLTKARLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF    KPK+YTKCKS VK+TK R+D +++KKN+V KYLK+DIV+LLK+ LDYNAY RAE
Sbjct: 1   MFDGLFKPKFYTKCKSLVKITKTRVDTVKRKKNSVCKYLKNDIVDLLKNSLDYNAYGRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGIIFNQLSVLNKQSECPDECKEAVASLIYAAARFADLPELR 120
           G + E+  L CYE +++FC  + + +S+L K   CPDEC+EA++SL+YAAAR +++PELR
Sbjct: 61  GLIEEKRRLACYEFLEQFCNCVASNVSLLQKSIRCPDECREAISSLVYAAARVSEVPELR 120

Query: 121 ELRNLFTGKYGSSFESFTNKELIEKSNTIALTKEMKLQLLQEIVQESAIDWNSKALEQQL 180
           +LR+LF  +YG++ + F N E +E+      +KEMK++LLQEI +E +I W++K+LEQ+L
Sbjct: 121 DLRSLFAERYGNTLDQFVNPEFVERFKAEPPSKEMKVELLQEIAREYSIKWDAKSLEQRL 180

Query: 181 YTPPQN---EPDCDRRSTEKRNKTKVAFVPVHERKKSSLEKKDNSDDDSMFDSRSEGNTT 240
           YTPP +     + +   TEK N        + +R   S   +++S DDS   ++S  +T+
Sbjct: 181 YTPPHHHHVHTETEEPKTEKTNSEPEK--TMEKRSSLSFHGREDSMDDS---NKSIMSTS 240

Query: 241 ETSTGDSSTDQDVRKGVSEDEVEDQKPFYLRVIRP-PYLKTKPTNKETMEEARKATDHIE 300
           E  +   ST      G  +D   + KPFY R + P PY K K   +E++ E +  T H+ 
Sbjct: 241 EEDS--MSTSGSCMTGPEDD--PEMKPFYYRFMTPAPYTKPKIEKQESLPE-KMTTSHLM 300

Query: 301 NDESNKQQPKPTSEEKPKPRSVRRRNVKPQPARDINIDDVGNSANDGVEKSSLSRNKGKE 360
           + ES      PT   KPKPRSVRRR   P P  +      G +    VE  ++ R    E
Sbjct: 301 DTES------PTG-GKPKPRSVRRRFANPPPEAETE----GEALKLKVESKNMMR--ASE 346

Query: 361 TMIGEEKGAG 367
           +M G+  G G
Sbjct: 361 SM-GKASGEG 346

BLAST of Tan0003654 vs. TAIR 10
Match: AT1G79910.2 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 161.0 bits (406), Expect = 2.3e-39
Identity = 117/311 (37.62%), Postives = 178/311 (57.23%), Query Frame = 0

Query: 60  EGFLVERNVLRCYELIDEFCGIIFNQLSVLNKQSECPDECKEAVASLIYAAARFADLPEL 119
           EG + E+  L CYE +++FC  + + +S+L K   CPDEC+EA++SL+YAAAR +++PEL
Sbjct: 46  EGLIEEKRRLACYEFLEQFCNCVASNVSLLQKSIRCPDECREAISSLVYAAARVSEVPEL 105

Query: 120 RELRNLFTGKYGSSFESFTNKELIEKSNTIALTKEMKLQLLQEIVQESAIDWNSKALEQQ 179
           R+LR+LF  +YG++ + F N E +E+      +KEMK++LLQEI +E +I W++K+LEQ+
Sbjct: 106 RDLRSLFAERYGNTLDQFVNPEFVERFKAEPPSKEMKVELLQEIAREYSIKWDAKSLEQR 165

Query: 180 LYTPPQN---EPDCDRRSTEKRNKTKVAFVPVHERKKSSLEKKDNSDDDSMFDSRSEGNT 239
           LYTPP +     + +   TEK N        + +R   S   +++S DDS   ++S  +T
Sbjct: 166 LYTPPHHHHVHTETEEPKTEKTNSEPEK--TMEKRSSLSFHGREDSMDDS---NKSIMST 225

Query: 240 TETSTGDSSTDQDVRKGVSEDEVEDQKPFYLRVIRP-PYLKTKPTNKETMEEARKATDHI 299
           +E  +   ST      G  +D   + KPFY R + P PY K K   +E++ E +  T H+
Sbjct: 226 SEEDS--MSTSGSCMTGPEDD--PEMKPFYYRFMTPAPYTKPKIEKQESLPE-KMTTSHL 285

Query: 300 ENDESNKQQPKPTSEEKPKPRSVRRRNVKPQPARDINIDDVGNSANDGVEKSSLSRNKGK 359
            + ES      PT   KPKPRSVRRR   P P  +      G +    VE  ++ R    
Sbjct: 286 MDTES------PTG-GKPKPRSVRRRFANPPPEAETE----GEALKLKVESKNMMR--AS 332

Query: 360 ETMIGEEKGAG 367
           E+M G+  G G
Sbjct: 346 ESM-GKASGEG 332

BLAST of Tan0003654 vs. TAIR 10
Match: AT1G52315.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 146.7 bits (369), Expect = 4.5e-35
Identity = 101/306 (33.01%), Postives = 170/306 (55.56%), Query Frame = 0

Query: 1   MFGMFLKPKYYTKCKSCVKLTKARLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF    KPK+Y K KS     K R+D +R+K+ A+++  K+DIV  LK+  D  AY RAE
Sbjct: 1   MFLRCFKPKFYKKSKSTTSYMKIRIDIVRRKRIAMVRNYKTDIVNFLKNGQDSEAYRRAE 60

Query: 61  GFLVERNVLRCYELIDEFCGIIFNQLSVLNKQSECPDECKEAVASLIYAAARFADLPELR 120
             L E  ++ CY+LI+ FC  I   LS++ K+ ECP+EC+EAV+SLIYA A   D+PEL+
Sbjct: 61  LLLEELRIISCYDLIERFCDCISENLSLMLKKRECPEECREAVSSLIYATAWVPDVPELK 120

Query: 121 ELRNLFTGKYGSSFESFTNKELIEKSNTI-ALTKEMKLQLLQEIVQESAIDWNSKALEQQ 180
           +LR +FT ++G+   S  N EL+EK+  +   ++E+K+Q ++++  E +I+W+   L+  
Sbjct: 121 DLRAVFTKRFGNFIASSVNHELVEKTELLRPPSRELKIQTVKDVANEFSINWDPTPLKLM 180

Query: 181 LY---TPPQNEPDCDRRSTEKRNKTKVAFVPVHERKKSSLEKKDNSDDDSMFDSRSEGNT 240
           L    +  Q +   + R+      T+ +F+  H   +S L       +    DS S+G+ 
Sbjct: 181 LLRETSALQIKDKVETRADYLNKDTEKSFMDEHSDDESVL------SESWRIDSLSKGSL 240

Query: 241 TETSTGDSSTDQDVRKGVSEDEVEDQKPFYLRVIRPPYLKTKPTNKETMEEARKATDHIE 300
           + +S+  SS+     + VS  + + +K     +I  P  K    N E  +E ++    I 
Sbjct: 241 SSSSSSSSSS----LRRVSVRKKKGKKILPYGIISSPDTKLGAGNDEKAQEEKEEKRKIA 296

Query: 301 NDESNK 303
           + E+++
Sbjct: 301 DQENSR 296

BLAST of Tan0003654 vs. TAIR 10
Match: AT4G32350.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 117.5 bits (293), Expect = 3.0e-26
Identity = 118/461 (25.60%), Postives = 219/461 (47.51%), Query Frame = 0

Query: 1   MFGMFLKPKYYTKCKSCVKLTKARLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAE 60
           MF  FL   +  K K  +KLTK R+D +R+K+NA +K+LK D+ +L+ +  DYNA++RA 
Sbjct: 1   MFDGFLGRGFAPKGKPLIKLTKNRIDVLRRKRNATIKFLKRDLADLIINGHDYNAFSRAG 60

Query: 61  GFLVERNVLRCYELIDEFCGIIFNQLSVLNKQSECPDECKEAVASLIYAAARFADLPELR 120
           G L E   L   + +++ C  ++ QLS + K  ECP++C+EA++SL++AA+ F++LPELR
Sbjct: 61  GLLDELRYLWSLDFVEQTCDFVYKQLSTMQKTPECPEDCREAISSLMFAASGFSELPELR 120

Query: 121 ELRNLFTGKYGSSFESFTNKELIEKSNTIALTKEMKLQLLQEIVQESAIDWNSKALEQQL 180
           ELR +F  KY  S   F N+EL+E  ++   + E K++L++++  E +I W+SK  E+++
Sbjct: 121 ELRQMFHEKYTDSLALFVNQELVENMSSKPFSMEKKVKLMEDVALEFSIRWDSKDFEKRI 180

Query: 181 Y-------------TPPQNEPDCDRRSTEKRNKTKVA--FVPVHERKKSSLEKKD---NS 240
                         T  + +P     +  KR + + +   V ++ +   + E++D    S
Sbjct: 181 VRQNSISVMETPKSTNDKYKPVDRNMALPKREEFEGSENGVSLNRKTAEASERRDPLFQS 240

Query: 241 DDDSMFDSRSEGNTTETSTGDSSTDQDVRKGVSEDEVEDQKPFYLRVIR-PPYLKTKPT- 300
           D +S  +         T    S       +  S+D   ++K FYL   + P   K +P  
Sbjct: 241 DKESYQNGLRGNQRGLTYKERSENVLHASRSESKDNKAERKEFYLHSKQNPAREKHQPIF 300

Query: 301 --------------------NKETMEEARKATDHI--ENDESNKQQPKPTSEEKPKPRSV 360
                               ++  + +A K T+ +  E  E   Q  +  S E+ +P   
Sbjct: 301 NEGDTIVMKVNYGNLGQGNGHRPGVVDAHKKTEFVASERKEFYSQSKQEPSRERHQPIFN 360

Query: 361 RRRNVKPQPARDINIDDVGNSANDGVEKSSLSRNKGKETMIGEEKGAGDDEGKMLDGLLM 420
               +  +   + ++   GN   +GV       +  K+  +   +       K  D L++
Sbjct: 361 DGDTIVMKVKHENHVQ--GNGHKNGVV------DLHKKIEVNASEKLKSSSSKRADKLVI 420

BLAST of Tan0003654 vs. TAIR 10
Match: AT4G35730.1 (Regulator of Vps4 activity in the MVB pathway protein )

HSP 1 Score: 115.5 bits (288), Expect = 1.1e-25
Identity = 123/472 (26.06%), Postives = 212/472 (44.92%), Query Frame = 0

Query: 12  TKCKSCVKLTKARLDAIRKKKNAVLKYLKSDIVELLKSRLDYNAYNRAEGFLVERNVLRC 71
           +KCK+  K+  AR+  IR K+  V+K ++ DI  LL+S  D  A  R E  + E+N+   
Sbjct: 29  SKCKTAAKMAVARIKLIRNKRLVVVKQMRRDIAVLLQSGQDATARIRVEHVIREQNIQAA 88

Query: 72  YELIDEFCGIIFNQLSVLNKQSECPDECKEAVASLIYAAARFADLPELRELRNLFTGKYG 131
            E+I+ FC +I ++L+++ KQ +CP + KE +ASLI+AA R +++PEL +LR++F  KYG
Sbjct: 89  NEIIELFCELIVSRLTIITKQKQCPVDLKEGIASLIFAAPRCSEIPELGDLRDIFAKKYG 148

Query: 132 SSFESF---------TNKELIEKSNTIALTKEMKLQLLQEIVQESAIDWNSKALEQQLYT 191
             F S           N+ LI+K +      E KL++++EI +E  +DW++   EQ+L  
Sbjct: 149 KDFVSAATDLRPSCGVNRMLIDKLSVRNPGGEYKLKIMKEIAKEFQVDWDTTETEQELLK 208

Query: 192 PPQNEPDCDRRSTEKRNKTKVAFVPVHERKKSSLEKKDNSDDDSMFDSRSEGNTTETSTG 251
           P +   D  R+           FV       SSL     + ++ +  +++   +T + + 
Sbjct: 209 PQEESIDGPRK-----------FV-----SASSLPVNRAAINEPIDPTKAVPRSTSSMSI 268

Query: 252 DSSTDQDVRKGVSEDEVEDQKPFYLRVIRPPYLKTKPTNKETMEEARKATDHIENDESNK 311
           ++          +  E+  Q     +V      +   +NKE    +  +T   ++   + 
Sbjct: 269 NTHYHDTESAAEAATELAKQAVAAAQVASLLATRRDSSNKEFSVSSDHSTHQKDSQYMDH 328

Query: 312 QQPKPTSEEKPKPRSVRRRNVKPQPARDINIDDVGNSANDGVEKSSLSRNKGKETMIGEE 371
               P S  + +         KP             + N G+ +     N G      EE
Sbjct: 329 HHHHPGSRRQSRDSETSSYYAKP------------GAENRGMGRRHSYNNPGINESDYEE 388

Query: 372 KGAGDDEGKMLDGLLMHYSKKKSHESNKAKGNLKF-QRQQEKDNVEPQKPKNTKTEDAFV 431
           +   + E +  + +   +S         A   +KF +    ++  EP +P   +      
Sbjct: 389 EYT-NTEAEAKETMRRRHSYNPRSVPPPATSEIKFDESDYYEEETEPDEPSQGRVS---- 448

Query: 432 STKAVSVPIDSTEPTEPVKKHTRSNSF---VHPKLPDYDQLAARFTALKEEK 471
                S+P +   P  P    +R +S    VHPKLPDYD LAARF A++  K
Sbjct: 449 -----SLPPNRAPPQAPQSGESRQDSSGHQVHPKLPDYDILAARFEAIRHSK 462