Tan0003503 (gene) Snake gourd v1

BLAST of Tan0003503 vs. ExPASy Swiss-Prot
Match: O80668 (Amino acid transporter AVT1A OS=Arabidopsis thaliana OX=3702 GN=AVT1A PE=2 SV=2)

HSP 1 Score: 392.9 bits (1008), Expect = 5.9e-108
Identity = 224/437 (51.26%), Postives = 294/437 (67.28%), Query Frame = 0

Query: 3   EKKDNHRDGDSMVFFQPQQEEEEDDIEMNNKGLESSDGSSGSFSGGYYSAAAVTTAVSGF 62
           E K+N ++  + V F  + +E+ +D+E N+   E+   +  S  G     A         
Sbjct: 2   EDKNNDKEKKTDVTF--EDDEDNEDLEDNSSKYENDSETDQSDLGDLPGDAVDRD----- 61

Query: 63  LRQGYNSASSSQ-QWPRSFREASDSYAITTSPTFGILRI--PSIIKSSFQN-GIESVLEF 122
                +    SQ QWP+SFRE +DSY I  SP FG LR   PS  ++S  N  +ES    
Sbjct: 62  --DDIDEPFISQVQWPQSFRETTDSYTIAASPIFGSLRSNPPSFYRASRSNLDVESKAPL 121

Query: 123 DVDADVDADVAAKAPLLTNYYGHEGHGLDYRISSSQQLPT-PHGCSFTQTIFNGMNFMAG 182
             +   D+D   KA    + + H+G       S +++LP   +GCS  QTIFN +N MAG
Sbjct: 122 LPERHDDSD---KASATQSAWSHKG-------SFAEELPIGGYGCSVIQTIFNAINVMAG 181

Query: 183 VGLLSTPYTVKEAGWGSLGVLLVFAFVCYFTAMLMKFCFQ-KTGHFKIMTFPDLGQAAFG 242
           VGLLSTPYTVKEAGW S+ +LL+FA +C +TA LMK CF+ KTG   I+T+PD+G+AAFG
Sbjct: 182 VGLLSTPYTVKEAGWASMVILLLFAVICCYTATLMKDCFENKTG---IITYPDIGEAAFG 241

Query: 243 TFGRLFVSILLYLELYCCCIEFIILEEDNLSSLFPNAWLNLGGLHLDSMHLFGIITVLVV 302
            +GR+ + +LLY ELY  C+EFIILE DNL+ LFP   L+L G  LDS HLFGI+T L+V
Sbjct: 242 KYGRILICMLLYTELYSYCVEFIILEGDNLTGLFPGTSLDLLGFRLDSKHLFGILTALIV 301

Query: 303 LPTVWLRDLRWISYISAGGVLATTVIILTIAYLGTFGGIGFHEVEAGEIVNWKGIPFAIG 362
           LPTVWL+DLR ISY+SAGGV+AT +I +++ +LGT GGIGFH    G+ V W GIPFAIG
Sbjct: 302 LPTVWLKDLRIISYLSAGGVIATALIAVSVFFLGTTGGIGFH--HTGQAVKWNGIPFAIG 361

Query: 363 AYGFCFSGHTLFPNLYHSMADKTKFTKALLICFVFCVLIYGGIAVMGFFMFGQSILSQIT 422
            YGFC+SGH++FPN+Y SMADKTKF KA++ CF+ CVL+YGG+A+MG+ MFG++ LSQIT
Sbjct: 362 IYGFCYSGHSVFPNIYQSMADKTKFNKAVITCFIICVLLYGGVAIMGYLMFGEATLSQIT 414

Query: 423 LNMPQHALASNVAKWTT 434
           LNMPQ    S VA+WTT
Sbjct: 422 LNMPQDQFFSKVAQWTT 414

BLAST of Tan0003503 vs. ExPASy Swiss-Prot
Match: F4IUW3 (Amino acid transporter AVT1C OS=Arabidopsis thaliana OX=3702 GN=AVT1C PE=1 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 1.9e-82
Identity = 178/447 (39.82%), Postives = 255/447 (57.05%), Query Frame = 0

Query: 7   NHRDGDSMVFFQPQQEEEEDDIEMNNKGLESSDGSSGSFSGGYYSAAAVTTAVSGFLRQG 66
           NH   D   + + + E++  D       +E   GS    S   Y              Q 
Sbjct: 2   NHVPSDQSFYIESEDEDDRKDY------VEEDGGSHSDSSDDVYDE-----------NQA 61

Query: 67  YNSASS-SQQWPRSFREASDSYAITTSPTFGILRIPSIIK--SSF----------QNGIE 126
           +   SS +  WP+S+R++ D Y+   SP  G L   S+ +  SSF             + 
Sbjct: 62  HIKPSSYTTAWPQSYRQSIDLYSSVPSPGIGFLGNNSMTRFGSSFLSSGLIRRHTPESLP 121

Query: 127 SVLEFDVDADVDADVAAK-----APLLTNYYGHEGHGLDYRISS--SQQLPTPHGCSFTQ 186
           +V +  ++   D     K       LL+      G       SS  S ++P     S+ Q
Sbjct: 122 TVTKPLLEEQADEQALPKHRLSSQGLLSPIPSRRGSMRKDEKSSMVSHEIPMSRNSSYGQ 181

Query: 187 TIFNGMNFMAGVGLLSTPYTVKEAGWGSLGVLLVFAFVCYFTAMLMKFCFQKTGHFKIMT 246
            + NG+N + GVG+LSTPY  KE GW  L +L V+  + ++T +L+++C       +  T
Sbjct: 182 AVLNGLNVLCGVGILSTPYAAKEGGWLGLMILFVYGLLSFYTGILLRYCLDSESDLE--T 241

Query: 247 FPDLGQAAFGTFGRLFVSILLYLELYCCCIEFIILEEDNLSSLFPNAWLNLGGLHLDSMH 306
           +PD+GQAAFGT GR+FVSI+LYLELY CC+E+IILE DNLSSL+PNA L++GG  LD+ H
Sbjct: 242 YPDIGQAAFGTTGRIFVSIVLYLELYACCVEYIILESDNLSSLYPNAALSIGGFQLDARH 301

Query: 307 LFGIITVLVVLPTVWLRDLRWISYISAGGVLATTVIILTIAYLGTFGGIGFHEVEAGEIV 366
           LF ++T L VLPTVWLRDL  +SYISAGGV+A+ +++L + ++G    +G H    G  +
Sbjct: 302 LFALLTTLAVLPTVWLRDLSVLSYISAGGVIASVLVVLCLFWIGLVDEVGIH--SKGTTL 361

Query: 367 NWKGIPFAIGAYGFCFSGHTLFPNLYHSMADKTKFTKALLICFVFCVLIYGGIAVMGFFM 426
           N   +P AIG YG+C+SGH +FPN+Y SMA  +++   LL CF  C L+Y G+AVMG+ M
Sbjct: 362 NLSTLPVAIGLYGYCYSGHAVFPNIYTSMAKPSQYPAVLLTCFGICTLMYAGVAVMGYTM 421

Query: 427 FGQSILSQITLNMPQHALASNVAKWTT 434
           FG+S  SQ TLN+PQ  +A+ +A WTT
Sbjct: 422 FGESTQSQFTLNLPQDLIATKIAVWTT 427

BLAST of Tan0003503 vs. ExPASy Swiss-Prot
Match: F4JE35 (Amino acid transporter AVT1B OS=Arabidopsis thaliana OX=3702 GN=AVT1B PE=3 SV=2)

HSP 1 Score: 293.9 bits (751), Expect = 3.7e-78
Identity = 169/443 (38.15%), Postives = 250/443 (56.43%), Query Frame = 0

Query: 7   NHRDGDSMVFFQPQQEEEEDDIEMNNKGLESSDGSSGSFSGGYYSAAAVTTAVSGFLRQG 66
           NH   D  ++    + ++ DD   +    E  DG+    S  Y       +  S +    
Sbjct: 2   NHSTSDQSLYI---ESDDGDDERKHLSDDEDDDGTLSDTSDAYNQNQHHLSKASPY---- 61

Query: 67  YNSASSSQQWPRSFREASDSYAITTSPTFGILRIPSIIK--SSFQNGIESVLEFDVDADV 126
                 S  WP+S+R++ D +    SP  G L   S+ +  SSF   + S L        
Sbjct: 62  ------STAWPKSYRQSIDMFGSVPSPNLGFLANSSMSRRGSSF---MSSTL-------T 121

Query: 127 DADVAAKAPLLTNYYGHEGHGLDYRISSSQQLPT--------------PHGCSFTQTIFN 186
                   P +T     +     +++S+   LP+               +  SF Q + N
Sbjct: 122 RRHTPESLPCVTKPLLEDEEAPKHKLSTHSLLPSKPSSSMVVSHDMGISNDSSFGQAVLN 181

Query: 187 GMNFMAGVGLLSTPYTVKEAGWGSLGVLLVFAFVCYFTAMLMKFCFQKTGHFKIMTFPDL 246
           G+N + GVG+LSTPY VKE GW  L +L  F  +C++T +L+++C     H  + T+PD+
Sbjct: 182 GVNVLCGVGILSTPYAVKEGGWLGLIILFAFGILCFYTGLLLRYCLD--SHPDVQTYPDI 241

Query: 247 GQAAFGTFGRLFVSILLYLELYCCCIEFIILEEDNLSSLFPNAWLNLGGLHLDSMHLFGI 306
           G AAFG+ GR+ VS++LY+ELY   +E+IILE DNLSS+FPNA L++GG HLD+  LF +
Sbjct: 242 GHAAFGSTGRILVSVILYMELYAMSVEYIILEGDNLSSMFPNASLSIGGFHLDAPRLFAL 301

Query: 307 ITVLVVLPTVWLRDLRWISYISAGGVLATTVIILTIAYLGTFGGIGFHEVEAGEIVNWKG 366
           +T L VLPTVWLRDL  +SYISAGGV+A+ +++L + ++G    +G H    G  +N   
Sbjct: 302 LTTLAVLPTVWLRDLSVLSYISAGGVIASVLVVLCLFWVGLVDDVGIH--SKGTPLNLAT 361

Query: 367 IPFAIGAYGFCFSGHTLFPNLYHSMADKTKFTKALLICFVFCVLIYGGIAVMGFFMFGQS 426
           +P ++G YG+C+SGH +FPN+Y SMA  ++F+  LL  F  C L+Y G+AVMG+ MFG+S
Sbjct: 362 LPVSVGLYGYCYSGHGVFPNIYTSMAKPSQFSAVLLASFGICTLMYAGVAVMGYSMFGES 417

Query: 427 ILSQITLNMPQHALASNVAKWTT 434
             SQ TLN+PQ  +AS +A WTT
Sbjct: 422 TESQFTLNLPQDLVASKIALWTT 417

BLAST of Tan0003503 vs. ExPASy Swiss-Prot
Match: Q8LPF4 (Amino acid transporter AVT1E OS=Arabidopsis thaliana OX=3702 GN=AVT1E PE=2 SV=1)

HSP 1 Score: 285.0 bits (728), Expect = 1.7e-75
Identity = 158/371 (42.59%), Postives = 222/371 (59.84%), Query Frame = 0

Query: 76  WPRSFREASDSYAITTSPT-------FGILRIPSIIKSSFQNGIESVLEFDVDADVDADV 135
           WPRS+R++ D     T PT       F   R  S+  S   +  +  L  D D    + V
Sbjct: 48  WPRSYRQSMDLLTGVTPPTSTSFVSSFRQRRQSSVFGSFTSSPSKQQLLIDKDEIQSSVV 107

Query: 136 AAKAPLLTNYYGHEGHGLDYRISSSQQLPTP---HGCSFTQTIFNGMNFMAGVGLLSTPY 195
           ++    L ++          ++S    L TP     C+F+Q++ NG+N + GV LL+ PY
Sbjct: 108 SSIKSFLASH---------LQLSVPGDLLTPQENRSCTFSQSVLNGINVLCGVALLTMPY 167

Query: 196 TVKEAGWGSLGVLLVFAFVCYFTAMLMKFCFQKTGHFKIMTFPDLGQAAFGTFGRLFVSI 255
            VKE GW  L +L  F  + ++T +L+K C + +    I T+PD+GQAAFGT GR+ VSI
Sbjct: 168 AVKEGGWLGLFILFSFGIITFYTGILLKRCLENSP--GIHTYPDIGQAAFGTTGRILVSI 227

Query: 256 LLYLELYCCCIEFIILEEDNLSSLFPNAWLNLGGLHLDSMHLFGIITVLVVLPTVWLRDL 315
           LLY+ELY  C+E+II+  DNLS +FPN  L + G  LDS  +F I T L+VLPTVWL+DL
Sbjct: 228 LLYVELYASCVEYIIMMSDNLSRMFPNTSLYINGFSLDSTQVFAITTTLIVLPTVWLKDL 287

Query: 316 RWISYISAGGVLATTVIILTIAYLGTFGGIGFHEVEAGEIVNWKGIPFAIGAYGFCFSGH 375
             +SY+SAGGV+++ ++ L + + G+  G+GFH   +G+ ++   IP AIG YGF F  H
Sbjct: 288 SLLSYLSAGGVISSILLALCLFWAGSVDGVGFH--ISGQALDITNIPVAIGIYGFGFGSH 347

Query: 376 TLFPNLYHSMADKTKFTKALLICFVFCVLIYGGIAVMGFFMFGQSILSQITLNMPQHALA 435
           ++FPN+Y SM + +KF   LLI F FC L Y  +AV GF MFG +I SQ TLNMP H  +
Sbjct: 348 SVFPNIYSSMKEPSKFPTVLLISFAFCTLFYIAVAVCGFTMFGDAIQSQFTLNMPPHFTS 405

Query: 436 SNVAKWTTNCT 437
           S +A WT   T
Sbjct: 408 SKIAVWTAVVT 405

BLAST of Tan0003503 vs. ExPASy Swiss-Prot
Match: Q1PER9 (Amino acid transporter AVT1G OS=Arabidopsis thaliana OX=3702 GN=AVT1G PE=2 SV=1)

HSP 1 Score: 284.6 bits (727), Expect = 2.3e-75
Identity = 157/370 (42.43%), Postives = 228/370 (61.62%), Query Frame = 0

Query: 69  SASSSQQWPRSFREASDSYAITTSPTFGILRIPSIIKSSFQNGIESVLEFDVD--ADVDA 128
           S + S  WPRS+R++ D     T PT   +   S  ++SF + + S+ +      A+  A
Sbjct: 39  SQNDSAPWPRSYRQSVDILTGVTPPTISFIHRRS-SQTSFTSSVASLYKRRPTSIANSFA 98

Query: 129 DVAAKAPLLTN----YYGHEGHGLDYRISSSQQLPTPHGCSFTQTIFNGMNFMAGVGLLS 188
              +K PLL+      +     GL    ++      P+ CSF Q++ NG+N + G+ LL+
Sbjct: 99  SSTSKQPLLSEKDDVLFLSSQIGLS---NTDLSYGEPNFCSFPQSVLNGINVLCGISLLT 158

Query: 189 TPYTVKEAGWGSLGVLLVFAFVCYFTAMLMKFCFQKTGHFKIMTFPDLGQAAFGTFGRLF 248
            PY VKE GW  L +LL FA +  +T +L+K C + +   +  T+PD+GQAAFG  GRL 
Sbjct: 159 MPYAVKEGGWLGLCILLSFAIITCYTGILLKRCLESSSDLR--TYPDIGQAAFGFTGRLI 218

Query: 249 VSILLYLELYCCCIEFIILEEDNLSSLFPNAWLNLGGLHLDSMHLFGIITVLVVLPTVWL 308
           +SILLY+ELY CC+E+II+  DNLS +FPN  LN+ G+ LDS  +F I   L+VLPTVWL
Sbjct: 219 ISILLYMELYVCCVEYIIMMSDNLSRVFPNITLNIVGVSLDSPQIFAISATLIVLPTVWL 278

Query: 309 RDLRWISYISAGGVLATTVIILTIAYLGTFGGIGFHEVEAGEIVNWKGIPFAIGAYGFCF 368
           +DL  +SY+SAGGV  + ++ L + ++G+  G+GFH    G+ ++   +P AIG +GF F
Sbjct: 279 KDLSLLSYLSAGGVFVSILLALCLFWVGSVDGVGFH--TGGKSLDLANLPVAIGIFGFGF 338

Query: 369 SGHTLFPNLYHSMADKTKFTKALLICFVFCVLIYGGIAVMGFFMFGQSILSQITLNMPQH 428
           SGH + P++Y SM + +KF   LLI F FCV  Y  +A+ G+ MFG++I SQ TLNMPQ 
Sbjct: 339 SGHAVLPSIYSSMKEPSKFPLVLLISFGFCVFFYIVVAICGYSMFGEAIQSQFTLNMPQQ 398

Query: 429 ALASNVAKWT 433
             AS +A WT
Sbjct: 399 YTASKIAVWT 400

BLAST of Tan0003503 vs. NCBI nr
Match: XP_038905343.1 (amino acid transporter AVT1A-like isoform X1 [Benincasa hispida])

HSP 1 Score: 696.8 bits (1797), Expect = 1.5e-196
Identity = 357/442 (80.77%), Postives = 387/442 (87.56%), Query Frame = 0

Query: 1   MAEKKDNHRD----GDSMVFFQPQ----QEEEEDDIEMNNKGLESSDGSS-GSFSGGYYS 60
           MAEKK+N  D     DS +FF+PQ    QE++EDD+EMNNKG+E S  SS  SFS G  S
Sbjct: 1   MAEKKNNRPDYYDGQDSELFFEPQYNDGQEDQEDDVEMNNKGMEGSSVSSCSSFSDGDDS 60

Query: 61  AAAVTTAVSGFLRQGYNSASSSQQWPRSFREASDSYAITTSPTFGILRIPSIIKSSFQNG 120
             AV  AV+  L +G +SAS+SQQWP+SFREASDSYAITTSPTFGILRIP+IIKSSF NG
Sbjct: 61  TVAV-AAVNRCLDRGCSSASASQQWPQSFREASDSYAITTSPTFGILRIPNIIKSSFHNG 120

Query: 121 IESVLEFDVDADVDADVAAKAPLLTNYYGHEGHGLDYRISSSQQLPTPHGCSFTQTIFNG 180
           IES+ E DV+ + D ++ AKAPLL NYYGHE H LDY  SSSQ L  PHGC+FTQTIFNG
Sbjct: 121 IESLFESDVNVNTDTNIDAKAPLLNNYYGHEEHNLDYNNSSSQSLSAPHGCTFTQTIFNG 180

Query: 181 MNFMAGVGLLSTPYTVKEAGWGSLGVLLVFAFVCYFTAMLMKFCFQKTGHFKIMTFPDLG 240
           MNFMAG+GLLSTPYTVKE GWGSLGVLLVFA VC+FTAMLMK+CF+K   FKI TFPDLG
Sbjct: 181 MNFMAGIGLLSTPYTVKEGGWGSLGVLLVFALVCFFTAMLMKYCFEKRSQFKIRTFPDLG 240

Query: 241 QAAFGTFGRLFVSILLYLELYCCCIEFIILEEDNLSSLFPNAWLNLGGLHLDSMHLFGII 300
           +AAFGTFGRLFVSILLYLELYCCC+EFIILEEDNLSSLFPN WLN GGLHLDSMHLFGII
Sbjct: 241 EAAFGTFGRLFVSILLYLELYCCCVEFIILEEDNLSSLFPNTWLNFGGLHLDSMHLFGII 300

Query: 301 TVLVVLPTVWLRDLRWISYISAGGVLATTVIILTIAYLGTFGGIGFHEVEAGEIVNWKGI 360
           TVL++LPTVWLRDLRWISYISAGGVLATT +ILTIAYLGT GGIGF+EVEAGEIVNWKGI
Sbjct: 301 TVLLLLPTVWLRDLRWISYISAGGVLATTAVILTIAYLGTIGGIGFNEVEAGEIVNWKGI 360

Query: 361 PFAIGAYGFCFSGHTLFPNLYHSMADKTKFTKALLICFVFCVLIYGGIAVMGFFMFGQSI 420
           PFAIGAYGFCFSGHTLFPNLYHSMADKTKFTKALLICFVFCV IYGG+AVMGF MFGQSI
Sbjct: 361 PFAIGAYGFCFSGHTLFPNLYHSMADKTKFTKALLICFVFCVFIYGGVAVMGFLMFGQSI 420

Query: 421 LSQITLNMPQHALASNVAKWTT 434
           LSQITLNMPQHALASNVAKWTT
Sbjct: 421 LSQITLNMPQHALASNVAKWTT 441

BLAST of Tan0003503 vs. NCBI nr
Match: XP_038905344.1 (amino acid transporter AVT1A-like isoform X2 [Benincasa hispida])

HSP 1 Score: 696.8 bits (1797), Expect = 1.5e-196
Identity = 357/442 (80.77%), Postives = 387/442 (87.56%), Query Frame = 0

Query: 1   MAEKKDNHRD----GDSMVFFQPQ----QEEEEDDIEMNNKGLESSDGSS-GSFSGGYYS 60
           MAEKK+N  D     DS +FF+PQ    QE++EDD+EMNNKG+E S  SS  SFS G  S
Sbjct: 1   MAEKKNNRPDYYDGQDSELFFEPQYNDGQEDQEDDVEMNNKGMEGSSVSSCSSFSDGDDS 60

Query: 61  AAAVTTAVSGFLRQGYNSASSSQQWPRSFREASDSYAITTSPTFGILRIPSIIKSSFQNG 120
             AV  AV+  L +G +SAS+SQQWP+SFREASDSYAITTSPTFGILRIP+IIKSSF NG
Sbjct: 61  TVAV-AAVNRCLDRGCSSASASQQWPQSFREASDSYAITTSPTFGILRIPNIIKSSFHNG 120

Query: 121 IESVLEFDVDADVDADVAAKAPLLTNYYGHEGHGLDYRISSSQQLPTPHGCSFTQTIFNG 180
           IES+ E DV+ + D ++ AKAPLL NYYGHE H LDY  SSSQ L  PHGC+FTQTIFNG
Sbjct: 121 IESLFESDVNVNTDTNIDAKAPLLNNYYGHEEHNLDYNNSSSQSLSAPHGCTFTQTIFNG 180

Query: 181 MNFMAGVGLLSTPYTVKEAGWGSLGVLLVFAFVCYFTAMLMKFCFQKTGHFKIMTFPDLG 240
           MNFMAG+GLLSTPYTVKE GWGSLGVLLVFA VC+FTAMLMK+CF+K   FKI TFPDLG
Sbjct: 181 MNFMAGIGLLSTPYTVKEGGWGSLGVLLVFALVCFFTAMLMKYCFEKRSQFKIRTFPDLG 240

Query: 241 QAAFGTFGRLFVSILLYLELYCCCIEFIILEEDNLSSLFPNAWLNLGGLHLDSMHLFGII 300
           +AAFGTFGRLFVSILLYLELYCCC+EFIILEEDNLSSLFPN WLN GGLHLDSMHLFGII
Sbjct: 241 EAAFGTFGRLFVSILLYLELYCCCVEFIILEEDNLSSLFPNTWLNFGGLHLDSMHLFGII 300

Query: 301 TVLVVLPTVWLRDLRWISYISAGGVLATTVIILTIAYLGTFGGIGFHEVEAGEIVNWKGI 360
           TVL++LPTVWLRDLRWISYISAGGVLATT +ILTIAYLGT GGIGF+EVEAGEIVNWKGI
Sbjct: 301 TVLLLLPTVWLRDLRWISYISAGGVLATTAVILTIAYLGTIGGIGFNEVEAGEIVNWKGI 360

Query: 361 PFAIGAYGFCFSGHTLFPNLYHSMADKTKFTKALLICFVFCVLIYGGIAVMGFFMFGQSI 420
           PFAIGAYGFCFSGHTLFPNLYHSMADKTKFTKALLICFVFCV IYGG+AVMGF MFGQSI
Sbjct: 361 PFAIGAYGFCFSGHTLFPNLYHSMADKTKFTKALLICFVFCVFIYGGVAVMGFLMFGQSI 420

Query: 421 LSQITLNMPQHALASNVAKWTT 434
           LSQITLNMPQHALASNVAKWTT
Sbjct: 421 LSQITLNMPQHALASNVAKWTT 441

BLAST of Tan0003503 vs. NCBI nr
Match: XP_022934675.1 (amino acid transporter AVT1A-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 674.5 bits (1739), Expect = 7.8e-190
Identity = 343/431 (79.58%), Postives = 381/431 (88.40%), Query Frame = 0

Query: 7   NHRDGDSMVFFQPQ----QEEEEDDIEMNNKGLESSDGSSGSFSGGYYSAAAVTTAVSGF 66
           + RD DS +F QP     QE +EDD+EMNNKG ESS  S GSFS G  S+AAV +     
Sbjct: 3   DRRDQDSELFLQPPYNGGQENQEDDMEMNNKGTESSGSSCGSFSDGDNSSAAVDSC---- 62

Query: 67  LRQGYNSASSSQQWPRSFREASDSYAITTSPTFGILRIPSIIKSSFQNGIESVLEFDVDA 126
           LR+G +SAS+SQQWP+SFREASDSYAIT SPTFGIL+IP+IIKSSFQNGIES+LE +++ 
Sbjct: 63  LRRGCSSASASQQWPQSFREASDSYAITNSPTFGILQIPTIIKSSFQNGIESLLESEINE 122

Query: 127 DVDADVAAKAPLLTNYYGHEGHGLDYRISSSQQLPTPHGCSFTQTIFNGMNFMAGVGLLS 186
           DVD++   K PLLTNYY  EGH L+Y ISS+  + +PHGC+FTQTIFNG+NFMAGVGLLS
Sbjct: 123 DVDSN--GKTPLLTNYYDAEGHKLEYEISSTPSISSPHGCTFTQTIFNGVNFMAGVGLLS 182

Query: 187 TPYTVKEAGWGSLGVLLVFAFVCYFTAMLMKFCFQKTGHFKIMTFPDLGQAAFGTFGRLF 246
           TPYTVKE GWGSLGVLLVFA +CYFTAMLMK+CF+K G   I+TFPDLGQAAFGTFGRLF
Sbjct: 183 TPYTVKEGGWGSLGVLLVFAIMCYFTAMLMKYCFEKRGQINIVTFPDLGQAAFGTFGRLF 242

Query: 247 VSILLYLELYCCCIEFIILEEDNLSSLFPNAWLNLGGLHLDSMHLFGIITVLVVLPTVWL 306
           VSI+LYLELYCCCIEFIILEEDNLSSLFPNA LNLGGLHLDS HLFGI+T L+VLPTV L
Sbjct: 243 VSIILYLELYCCCIEFIILEEDNLSSLFPNARLNLGGLHLDSNHLFGIVTALLVLPTVCL 302

Query: 307 RDLRWISYISAGGVLATTVIILTIAYLGTFGGIGFHEVEAGEIVNWKGIPFAIGAYGFCF 366
           RDLRWISY+SAGGV+ATTVIILTIAYLGT GGIGFHEVEAGE+VNWKGIPFAIGAYGFCF
Sbjct: 303 RDLRWISYLSAGGVVATTVIILTIAYLGTLGGIGFHEVEAGEVVNWKGIPFAIGAYGFCF 362

Query: 367 SGHTLFPNLYHSMADKTKFTKALLICFVFCVLIYGGIAVMGFFMFGQSILSQITLNMPQH 426
           SGHTLFPNLYHSMADKTKFTKALL+CFVFCVLIYGG+AVMGF MFGQ+ILSQITLNMPQH
Sbjct: 363 SGHTLFPNLYHSMADKTKFTKALLVCFVFCVLIYGGVAVMGFLMFGQTILSQITLNMPQH 422

Query: 427 ALASNVAKWTT 434
           ALAS+VAKWTT
Sbjct: 423 ALASSVAKWTT 427

BLAST of Tan0003503 vs. NCBI nr
Match: XP_022934677.1 (amino acid transporter AVT1A-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 674.5 bits (1739), Expect = 7.8e-190
Identity = 343/431 (79.58%), Postives = 381/431 (88.40%), Query Frame = 0

Query: 7   NHRDGDSMVFFQPQ----QEEEEDDIEMNNKGLESSDGSSGSFSGGYYSAAAVTTAVSGF 66
           + RD DS +F QP     QE +EDD+EMNNKG ESS  S GSFS G  S+AAV +     
Sbjct: 3   DRRDQDSELFLQPPYNGGQENQEDDMEMNNKGTESSGSSCGSFSDGDNSSAAVDSC---- 62

Query: 67  LRQGYNSASSSQQWPRSFREASDSYAITTSPTFGILRIPSIIKSSFQNGIESVLEFDVDA 126
           LR+G +SAS+SQQWP+SFREASDSYAIT SPTFGIL+IP+IIKSSFQNGIES+LE +++ 
Sbjct: 63  LRRGCSSASASQQWPQSFREASDSYAITNSPTFGILQIPTIIKSSFQNGIESLLESEINE 122

Query: 127 DVDADVAAKAPLLTNYYGHEGHGLDYRISSSQQLPTPHGCSFTQTIFNGMNFMAGVGLLS 186
           DVD++   K PLLTNYY  EGH L+Y ISS+  + +PHGC+FTQTIFNG+NFMAGVGLLS
Sbjct: 123 DVDSN--GKTPLLTNYYDAEGHKLEYEISSTPSISSPHGCTFTQTIFNGVNFMAGVGLLS 182

Query: 187 TPYTVKEAGWGSLGVLLVFAFVCYFTAMLMKFCFQKTGHFKIMTFPDLGQAAFGTFGRLF 246
           TPYTVKE GWGSLGVLLVFA +CYFTAMLMK+CF+K G   I+TFPDLGQAAFGTFGRLF
Sbjct: 183 TPYTVKEGGWGSLGVLLVFAIMCYFTAMLMKYCFEKRGQINIVTFPDLGQAAFGTFGRLF 242

Query: 247 VSILLYLELYCCCIEFIILEEDNLSSLFPNAWLNLGGLHLDSMHLFGIITVLVVLPTVWL 306
           VSI+LYLELYCCCIEFIILEEDNLSSLFPNA LNLGGLHLDS HLFGI+T L+VLPTV L
Sbjct: 243 VSIILYLELYCCCIEFIILEEDNLSSLFPNARLNLGGLHLDSNHLFGIVTALLVLPTVCL 302

Query: 307 RDLRWISYISAGGVLATTVIILTIAYLGTFGGIGFHEVEAGEIVNWKGIPFAIGAYGFCF 366
           RDLRWISY+SAGGV+ATTVIILTIAYLGT GGIGFHEVEAGE+VNWKGIPFAIGAYGFCF
Sbjct: 303 RDLRWISYLSAGGVVATTVIILTIAYLGTLGGIGFHEVEAGEVVNWKGIPFAIGAYGFCF 362

Query: 367 SGHTLFPNLYHSMADKTKFTKALLICFVFCVLIYGGIAVMGFFMFGQSILSQITLNMPQH 426
           SGHTLFPNLYHSMADKTKFTKALL+CFVFCVLIYGG+AVMGF MFGQ+ILSQITLNMPQH
Sbjct: 363 SGHTLFPNLYHSMADKTKFTKALLVCFVFCVLIYGGVAVMGFLMFGQTILSQITLNMPQH 422

Query: 427 ALASNVAKWTT 434
           ALAS+VAKWTT
Sbjct: 423 ALASSVAKWTT 427

BLAST of Tan0003503 vs. NCBI nr
Match: XP_022983751.1 (amino acid transporter AVT1A-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 666.4 bits (1718), Expect = 2.1e-187
Identity = 338/431 (78.42%), Postives = 374/431 (86.77%), Query Frame = 0

Query: 7   NHRDGDSMVFFQPQ----QEEEEDDIEMNNKGLESSDGSSGSFSGGYYSAAAVTTAVSGF 66
           + RD DS +F +P     Q+ +EDD+EMNNKG ESS  S GSFS G  S+AAV       
Sbjct: 3   DRRDQDSELFLEPPYNGGQQNQEDDMEMNNKGTESSGSSCGSFSDGDNSSAAVARC---- 62

Query: 67  LRQGYNSASSSQQWPRSFREASDSYAITTSPTFGILRIPSIIKSSFQNGIESVLEFDVDA 126
           LR+G +SAS+SQQWP++FREASDSYAIT SPTFGILRIPSIIKSSFQN IES+LE ++  
Sbjct: 63  LRRGCSSASASQQWPQTFREASDSYAITNSPTFGILRIPSIIKSSFQNRIESLLESEISD 122

Query: 127 DVDADVAAKAPLLTNYYGHEGHGLDYRISSSQQLPTPHGCSFTQTIFNGMNFMAGVGLLS 186
           D       K PLLTNYY  EGH L+Y ISS+  + +PHGC+FTQTIFNGMNFMAGVGLLS
Sbjct: 123 D------RKTPLLTNYYDDEGHQLEYEISSTPSVSSPHGCTFTQTIFNGMNFMAGVGLLS 182

Query: 187 TPYTVKEAGWGSLGVLLVFAFVCYFTAMLMKFCFQKTGHFKIMTFPDLGQAAFGTFGRLF 246
           TPYTVKE GWGSLGVLLVFA +CYFTAMLMK+CF+K G   I+TFPDLGQAAFGTFGRLF
Sbjct: 183 TPYTVKEGGWGSLGVLLVFAIMCYFTAMLMKYCFEKRGQINIVTFPDLGQAAFGTFGRLF 242

Query: 247 VSILLYLELYCCCIEFIILEEDNLSSLFPNAWLNLGGLHLDSMHLFGIITVLVVLPTVWL 306
           VSI+LYLELYCCCIEFIILEEDNLSSLFPNAWLNLGGLHLDS HLFGI+T L+VLPTV L
Sbjct: 243 VSIILYLELYCCCIEFIILEEDNLSSLFPNAWLNLGGLHLDSNHLFGIVTALLVLPTVCL 302

Query: 307 RDLRWISYISAGGVLATTVIILTIAYLGTFGGIGFHEVEAGEIVNWKGIPFAIGAYGFCF 366
           RDLRWISY+SAGGV+ATTVI+LTIAYLGT GGIGF EVEAGE+VNWKGIPF+IGAYGFCF
Sbjct: 303 RDLRWISYLSAGGVVATTVIVLTIAYLGTLGGIGFREVEAGEVVNWKGIPFSIGAYGFCF 362

Query: 367 SGHTLFPNLYHSMADKTKFTKALLICFVFCVLIYGGIAVMGFFMFGQSILSQITLNMPQH 426
           SGHTLFPNLYHSMADKTK+TKALL+CFVFCVLIYGG+AVMGF MFGQ+ILSQITLNMPQH
Sbjct: 363 SGHTLFPNLYHSMADKTKYTKALLVCFVFCVLIYGGVAVMGFLMFGQTILSQITLNMPQH 422

Query: 427 ALASNVAKWTT 434
           ALASNVAKWTT
Sbjct: 423 ALASNVAKWTT 423

BLAST of Tan0003503 vs. ExPASy TrEMBL
Match: A0A0A0LEV6 (Aa_trans domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G820500 PE=3 SV=1)

HSP 1 Score: 805.1 bits (2078), Expect = 1.8e-229
Identity = 406/524 (77.48%), Postives = 448/524 (85.50%), Query Frame = 0

Query: 1   MAEKKDNHRDGDSMVFF-QP------QQEEEEDDIEMNNKGL--ESSDGSSGSFSGGYYS 60
           MAEKK+N    DS +F  QP      Q E+E+DDIEMNN GL    S  S GSFS G   
Sbjct: 1   MAEKKNNQ---DSELFLDQPRYNNDGQDEDEDDDIEMNNNGLLESRSVSSCGSFSDGDDD 60

Query: 61  AAAVTTAVSGFLRQGYNSASSSQQWPRSFREASDSYAITTSPTFGILRIPSIIKSSFQNG 120
           +     A++  +R+G  S S+SQQWP+SFREASDSYAITTSPTFGILRIP+IIKSSFQNG
Sbjct: 61  STTTVAAINKCVRRGCKSTSTSQQWPQSFREASDSYAITTSPTFGILRIPNIIKSSFQNG 120

Query: 121 IESVLEFDVDADVDADVAAKAPLLTNYYGHEGHGLDYRISSSQQLPTPHGCSFTQTIFNG 180
           I   +E D   D+D +V AKAPLL +YYGHEGH L+Y+ISSSQ L TPHGC+F+QTIFNG
Sbjct: 121 I---VESD---DMDVEVDAKAPLLNSYYGHEGHNLEYKISSSQPLSTPHGCTFSQTIFNG 180

Query: 181 MNFMAGVGLLSTPYTVKEAGWGSLGVLLVFAFVCYFTAMLMKFCFQK-TGHFKIMTFPDL 240
           MNFMAGVGLLSTPYTVKEAGWGSLGVLLVFA VC+FTAMLMK+CF+K T  F I+TFPDL
Sbjct: 181 MNFMAGVGLLSTPYTVKEAGWGSLGVLLVFAIVCFFTAMLMKYCFEKTTSQFNIITFPDL 240

Query: 241 GQAAFGTFGRLFVSILLYLELYCCCIEFIILEEDNLSSLFPNAWLNLGGLHLDSMHLFGI 300
           G+AAFGTFGRLFVS+LLY+ELYCCC+EFIILEEDNLSSLFP+ WLN  G+HLDS++LFGI
Sbjct: 241 GEAAFGTFGRLFVSVLLYMELYCCCVEFIILEEDNLSSLFPDTWLNFVGIHLDSIYLFGI 300

Query: 301 ITVLVVLPTVWLRDLRWISYISAGGVLATTVIILTIAYLGTFGGIGFHEVEAGEIVNWKG 360
           IT L+VLPTVWLRDLRWISY+SAGGVLATTV+ILTIAYLGT GGIGFHE EA E+VNW G
Sbjct: 301 ITALLVLPTVWLRDLRWISYLSAGGVLATTVVILTIAYLGTVGGIGFHEAEAAELVNWNG 360

Query: 361 IPFAIGAYGFCFSGHTLFPNLYHSMADKTKFTKALLICFVFCVLIYGGIAVMGFFMFGQS 420
           IPFAIGAYGFCFSGHTLFPNLYHSMADKTKFTKALLICFVFCVLIYGG+A+MGF MFGQS
Sbjct: 361 IPFAIGAYGFCFSGHTLFPNLYHSMADKTKFTKALLICFVFCVLIYGGVAIMGFLMFGQS 420

Query: 421 ILSQITLNMPQHALASNVAKWTTNCTCYLFSLWCFPSPIFWSCDGFNWFPSLHTHCNNNT 480
           ILSQITLNMPQHALASNVAKWTTNCTCYLF L C  SP+ WSCDGFNW PSLHTHCNNNT
Sbjct: 421 ILSQITLNMPQHALASNVAKWTTNCTCYLFPLCCSTSPVLWSCDGFNWLPSLHTHCNNNT 480

Query: 481 SLLLFENHGEGSFQFTDHIMQGGNCFGHHWSNPWNILFIFTNSA 515
           SLL FENHGEGSFQ TD+ MQGGN  GHH SNPWNILF+FT S+
Sbjct: 481 SLLFFENHGEGSFQNTDNFMQGGNRVGHHSSNPWNILFVFTISS 515

BLAST of Tan0003503 vs. ExPASy TrEMBL
Match: A0A6J1F8E2 (amino acid transporter AVT1A-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111441796 PE=3 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 3.8e-190
Identity = 343/431 (79.58%), Postives = 381/431 (88.40%), Query Frame = 0

Query: 7   NHRDGDSMVFFQPQ----QEEEEDDIEMNNKGLESSDGSSGSFSGGYYSAAAVTTAVSGF 66
           + RD DS +F QP     QE +EDD+EMNNKG ESS  S GSFS G  S+AAV +     
Sbjct: 3   DRRDQDSELFLQPPYNGGQENQEDDMEMNNKGTESSGSSCGSFSDGDNSSAAVDSC---- 62

Query: 67  LRQGYNSASSSQQWPRSFREASDSYAITTSPTFGILRIPSIIKSSFQNGIESVLEFDVDA 126
           LR+G +SAS+SQQWP+SFREASDSYAIT SPTFGIL+IP+IIKSSFQNGIES+LE +++ 
Sbjct: 63  LRRGCSSASASQQWPQSFREASDSYAITNSPTFGILQIPTIIKSSFQNGIESLLESEINE 122

Query: 127 DVDADVAAKAPLLTNYYGHEGHGLDYRISSSQQLPTPHGCSFTQTIFNGMNFMAGVGLLS 186
           DVD++   K PLLTNYY  EGH L+Y ISS+  + +PHGC+FTQTIFNG+NFMAGVGLLS
Sbjct: 123 DVDSN--GKTPLLTNYYDAEGHKLEYEISSTPSISSPHGCTFTQTIFNGVNFMAGVGLLS 182

Query: 187 TPYTVKEAGWGSLGVLLVFAFVCYFTAMLMKFCFQKTGHFKIMTFPDLGQAAFGTFGRLF 246
           TPYTVKE GWGSLGVLLVFA +CYFTAMLMK+CF+K G   I+TFPDLGQAAFGTFGRLF
Sbjct: 183 TPYTVKEGGWGSLGVLLVFAIMCYFTAMLMKYCFEKRGQINIVTFPDLGQAAFGTFGRLF 242

Query: 247 VSILLYLELYCCCIEFIILEEDNLSSLFPNAWLNLGGLHLDSMHLFGIITVLVVLPTVWL 306
           VSI+LYLELYCCCIEFIILEEDNLSSLFPNA LNLGGLHLDS HLFGI+T L+VLPTV L
Sbjct: 243 VSIILYLELYCCCIEFIILEEDNLSSLFPNARLNLGGLHLDSNHLFGIVTALLVLPTVCL 302

Query: 307 RDLRWISYISAGGVLATTVIILTIAYLGTFGGIGFHEVEAGEIVNWKGIPFAIGAYGFCF 366
           RDLRWISY+SAGGV+ATTVIILTIAYLGT GGIGFHEVEAGE+VNWKGIPFAIGAYGFCF
Sbjct: 303 RDLRWISYLSAGGVVATTVIILTIAYLGTLGGIGFHEVEAGEVVNWKGIPFAIGAYGFCF 362

Query: 367 SGHTLFPNLYHSMADKTKFTKALLICFVFCVLIYGGIAVMGFFMFGQSILSQITLNMPQH 426
           SGHTLFPNLYHSMADKTKFTKALL+CFVFCVLIYGG+AVMGF MFGQ+ILSQITLNMPQH
Sbjct: 363 SGHTLFPNLYHSMADKTKFTKALLVCFVFCVLIYGGVAVMGFLMFGQTILSQITLNMPQH 422

Query: 427 ALASNVAKWTT 434
           ALAS+VAKWTT
Sbjct: 423 ALASSVAKWTT 427

BLAST of Tan0003503 vs. ExPASy TrEMBL
Match: A0A6J1F3H2 (amino acid transporter AVT1A-like isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111441796 PE=3 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 3.8e-190
Identity = 343/431 (79.58%), Postives = 381/431 (88.40%), Query Frame = 0

Query: 7   NHRDGDSMVFFQPQ----QEEEEDDIEMNNKGLESSDGSSGSFSGGYYSAAAVTTAVSGF 66
           + RD DS +F QP     QE +EDD+EMNNKG ESS  S GSFS G  S+AAV +     
Sbjct: 3   DRRDQDSELFLQPPYNGGQENQEDDMEMNNKGTESSGSSCGSFSDGDNSSAAVDSC---- 62

Query: 67  LRQGYNSASSSQQWPRSFREASDSYAITTSPTFGILRIPSIIKSSFQNGIESVLEFDVDA 126
           LR+G +SAS+SQQWP+SFREASDSYAIT SPTFGIL+IP+IIKSSFQNGIES+LE +++ 
Sbjct: 63  LRRGCSSASASQQWPQSFREASDSYAITNSPTFGILQIPTIIKSSFQNGIESLLESEINE 122

Query: 127 DVDADVAAKAPLLTNYYGHEGHGLDYRISSSQQLPTPHGCSFTQTIFNGMNFMAGVGLLS 186
           DVD++   K PLLTNYY  EGH L+Y ISS+  + +PHGC+FTQTIFNG+NFMAGVGLLS
Sbjct: 123 DVDSN--GKTPLLTNYYDAEGHKLEYEISSTPSISSPHGCTFTQTIFNGVNFMAGVGLLS 182

Query: 187 TPYTVKEAGWGSLGVLLVFAFVCYFTAMLMKFCFQKTGHFKIMTFPDLGQAAFGTFGRLF 246
           TPYTVKE GWGSLGVLLVFA +CYFTAMLMK+CF+K G   I+TFPDLGQAAFGTFGRLF
Sbjct: 183 TPYTVKEGGWGSLGVLLVFAIMCYFTAMLMKYCFEKRGQINIVTFPDLGQAAFGTFGRLF 242

Query: 247 VSILLYLELYCCCIEFIILEEDNLSSLFPNAWLNLGGLHLDSMHLFGIITVLVVLPTVWL 306
           VSI+LYLELYCCCIEFIILEEDNLSSLFPNA LNLGGLHLDS HLFGI+T L+VLPTV L
Sbjct: 243 VSIILYLELYCCCIEFIILEEDNLSSLFPNARLNLGGLHLDSNHLFGIVTALLVLPTVCL 302

Query: 307 RDLRWISYISAGGVLATTVIILTIAYLGTFGGIGFHEVEAGEIVNWKGIPFAIGAYGFCF 366
           RDLRWISY+SAGGV+ATTVIILTIAYLGT GGIGFHEVEAGE+VNWKGIPFAIGAYGFCF
Sbjct: 303 RDLRWISYLSAGGVVATTVIILTIAYLGTLGGIGFHEVEAGEVVNWKGIPFAIGAYGFCF 362

Query: 367 SGHTLFPNLYHSMADKTKFTKALLICFVFCVLIYGGIAVMGFFMFGQSILSQITLNMPQH 426
           SGHTLFPNLYHSMADKTKFTKALL+CFVFCVLIYGG+AVMGF MFGQ+ILSQITLNMPQH
Sbjct: 363 SGHTLFPNLYHSMADKTKFTKALLVCFVFCVLIYGGVAVMGFLMFGQTILSQITLNMPQH 422

Query: 427 ALASNVAKWTT 434
           ALAS+VAKWTT
Sbjct: 423 ALASSVAKWTT 427

BLAST of Tan0003503 vs. ExPASy TrEMBL
Match: A0A6J1J8D0 (amino acid transporter AVT1A-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482274 PE=3 SV=1)

HSP 1 Score: 666.4 bits (1718), Expect = 1.0e-187
Identity = 338/431 (78.42%), Postives = 374/431 (86.77%), Query Frame = 0

Query: 7   NHRDGDSMVFFQPQ----QEEEEDDIEMNNKGLESSDGSSGSFSGGYYSAAAVTTAVSGF 66
           + RD DS +F +P     Q+ +EDD+EMNNKG ESS  S GSFS G  S+AAV       
Sbjct: 3   DRRDQDSELFLEPPYNGGQQNQEDDMEMNNKGTESSGSSCGSFSDGDNSSAAVARC---- 62

Query: 67  LRQGYNSASSSQQWPRSFREASDSYAITTSPTFGILRIPSIIKSSFQNGIESVLEFDVDA 126
           LR+G +SAS+SQQWP++FREASDSYAIT SPTFGILRIPSIIKSSFQN IES+LE ++  
Sbjct: 63  LRRGCSSASASQQWPQTFREASDSYAITNSPTFGILRIPSIIKSSFQNRIESLLESEISD 122

Query: 127 DVDADVAAKAPLLTNYYGHEGHGLDYRISSSQQLPTPHGCSFTQTIFNGMNFMAGVGLLS 186
           D       K PLLTNYY  EGH L+Y ISS+  + +PHGC+FTQTIFNGMNFMAGVGLLS
Sbjct: 123 D------RKTPLLTNYYDDEGHQLEYEISSTPSVSSPHGCTFTQTIFNGMNFMAGVGLLS 182

Query: 187 TPYTVKEAGWGSLGVLLVFAFVCYFTAMLMKFCFQKTGHFKIMTFPDLGQAAFGTFGRLF 246
           TPYTVKE GWGSLGVLLVFA +CYFTAMLMK+CF+K G   I+TFPDLGQAAFGTFGRLF
Sbjct: 183 TPYTVKEGGWGSLGVLLVFAIMCYFTAMLMKYCFEKRGQINIVTFPDLGQAAFGTFGRLF 242

Query: 247 VSILLYLELYCCCIEFIILEEDNLSSLFPNAWLNLGGLHLDSMHLFGIITVLVVLPTVWL 306
           VSI+LYLELYCCCIEFIILEEDNLSSLFPNAWLNLGGLHLDS HLFGI+T L+VLPTV L
Sbjct: 243 VSIILYLELYCCCIEFIILEEDNLSSLFPNAWLNLGGLHLDSNHLFGIVTALLVLPTVCL 302

Query: 307 RDLRWISYISAGGVLATTVIILTIAYLGTFGGIGFHEVEAGEIVNWKGIPFAIGAYGFCF 366
           RDLRWISY+SAGGV+ATTVI+LTIAYLGT GGIGF EVEAGE+VNWKGIPF+IGAYGFCF
Sbjct: 303 RDLRWISYLSAGGVVATTVIVLTIAYLGTLGGIGFREVEAGEVVNWKGIPFSIGAYGFCF 362

Query: 367 SGHTLFPNLYHSMADKTKFTKALLICFVFCVLIYGGIAVMGFFMFGQSILSQITLNMPQH 426
           SGHTLFPNLYHSMADKTK+TKALL+CFVFCVLIYGG+AVMGF MFGQ+ILSQITLNMPQH
Sbjct: 363 SGHTLFPNLYHSMADKTKYTKALLVCFVFCVLIYGGVAVMGFLMFGQTILSQITLNMPQH 422

Query: 427 ALASNVAKWTT 434
           ALASNVAKWTT
Sbjct: 423 ALASNVAKWTT 423

BLAST of Tan0003503 vs. ExPASy TrEMBL
Match: A0A6J1J395 (amino acid transporter AVT1A-like isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111482274 PE=3 SV=1)

HSP 1 Score: 666.4 bits (1718), Expect = 1.0e-187
Identity = 338/431 (78.42%), Postives = 374/431 (86.77%), Query Frame = 0

Query: 7   NHRDGDSMVFFQPQ----QEEEEDDIEMNNKGLESSDGSSGSFSGGYYSAAAVTTAVSGF 66
           + RD DS +F +P     Q+ +EDD+EMNNKG ESS  S GSFS G  S+AAV       
Sbjct: 3   DRRDQDSELFLEPPYNGGQQNQEDDMEMNNKGTESSGSSCGSFSDGDNSSAAVARC---- 62

Query: 67  LRQGYNSASSSQQWPRSFREASDSYAITTSPTFGILRIPSIIKSSFQNGIESVLEFDVDA 126
           LR+G +SAS+SQQWP++FREASDSYAIT SPTFGILRIPSIIKSSFQN IES+LE ++  
Sbjct: 63  LRRGCSSASASQQWPQTFREASDSYAITNSPTFGILRIPSIIKSSFQNRIESLLESEISD 122

Query: 127 DVDADVAAKAPLLTNYYGHEGHGLDYRISSSQQLPTPHGCSFTQTIFNGMNFMAGVGLLS 186
           D       K PLLTNYY  EGH L+Y ISS+  + +PHGC+FTQTIFNGMNFMAGVGLLS
Sbjct: 123 D------RKTPLLTNYYDDEGHQLEYEISSTPSVSSPHGCTFTQTIFNGMNFMAGVGLLS 182

Query: 187 TPYTVKEAGWGSLGVLLVFAFVCYFTAMLMKFCFQKTGHFKIMTFPDLGQAAFGTFGRLF 246
           TPYTVKE GWGSLGVLLVFA +CYFTAMLMK+CF+K G   I+TFPDLGQAAFGTFGRLF
Sbjct: 183 TPYTVKEGGWGSLGVLLVFAIMCYFTAMLMKYCFEKRGQINIVTFPDLGQAAFGTFGRLF 242

Query: 247 VSILLYLELYCCCIEFIILEEDNLSSLFPNAWLNLGGLHLDSMHLFGIITVLVVLPTVWL 306
           VSI+LYLELYCCCIEFIILEEDNLSSLFPNAWLNLGGLHLDS HLFGI+T L+VLPTV L
Sbjct: 243 VSIILYLELYCCCIEFIILEEDNLSSLFPNAWLNLGGLHLDSNHLFGIVTALLVLPTVCL 302

Query: 307 RDLRWISYISAGGVLATTVIILTIAYLGTFGGIGFHEVEAGEIVNWKGIPFAIGAYGFCF 366
           RDLRWISY+SAGGV+ATTVI+LTIAYLGT GGIGF EVEAGE+VNWKGIPF+IGAYGFCF
Sbjct: 303 RDLRWISYLSAGGVVATTVIVLTIAYLGTLGGIGFREVEAGEVVNWKGIPFSIGAYGFCF 362

Query: 367 SGHTLFPNLYHSMADKTKFTKALLICFVFCVLIYGGIAVMGFFMFGQSILSQITLNMPQH 426
           SGHTLFPNLYHSMADKTK+TKALL+CFVFCVLIYGG+AVMGF MFGQ+ILSQITLNMPQH
Sbjct: 363 SGHTLFPNLYHSMADKTKYTKALLVCFVFCVLIYGGVAVMGFLMFGQTILSQITLNMPQH 422

Query: 427 ALASNVAKWTT 434
           ALASNVAKWTT
Sbjct: 423 ALASNVAKWTT 423

BLAST of Tan0003503 vs. TAIR 10
Match: AT2G41190.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 392.9 bits (1008), Expect = 4.2e-109
Identity = 224/437 (51.26%), Postives = 294/437 (67.28%), Query Frame = 0

Query: 3   EKKDNHRDGDSMVFFQPQQEEEEDDIEMNNKGLESSDGSSGSFSGGYYSAAAVTTAVSGF 62
           E K+N ++  + V F  + +E+ +D+E N+   E+   +  S  G     A         
Sbjct: 2   EDKNNDKEKKTDVTF--EDDEDNEDLEDNSSKYENDSETDQSDLGDLPGDAVDRD----- 61

Query: 63  LRQGYNSASSSQ-QWPRSFREASDSYAITTSPTFGILRI--PSIIKSSFQN-GIESVLEF 122
                +    SQ QWP+SFRE +DSY I  SP FG LR   PS  ++S  N  +ES    
Sbjct: 62  --DDIDEPFISQVQWPQSFRETTDSYTIAASPIFGSLRSNPPSFYRASRSNLDVESKAPL 121

Query: 123 DVDADVDADVAAKAPLLTNYYGHEGHGLDYRISSSQQLPT-PHGCSFTQTIFNGMNFMAG 182
             +   D+D   KA    + + H+G       S +++LP   +GCS  QTIFN +N MAG
Sbjct: 122 LPERHDDSD---KASATQSAWSHKG-------SFAEELPIGGYGCSVIQTIFNAINVMAG 181

Query: 183 VGLLSTPYTVKEAGWGSLGVLLVFAFVCYFTAMLMKFCFQ-KTGHFKIMTFPDLGQAAFG 242
           VGLLSTPYTVKEAGW S+ +LL+FA +C +TA LMK CF+ KTG   I+T+PD+G+AAFG
Sbjct: 182 VGLLSTPYTVKEAGWASMVILLLFAVICCYTATLMKDCFENKTG---IITYPDIGEAAFG 241

Query: 243 TFGRLFVSILLYLELYCCCIEFIILEEDNLSSLFPNAWLNLGGLHLDSMHLFGIITVLVV 302
            +GR+ + +LLY ELY  C+EFIILE DNL+ LFP   L+L G  LDS HLFGI+T L+V
Sbjct: 242 KYGRILICMLLYTELYSYCVEFIILEGDNLTGLFPGTSLDLLGFRLDSKHLFGILTALIV 301

Query: 303 LPTVWLRDLRWISYISAGGVLATTVIILTIAYLGTFGGIGFHEVEAGEIVNWKGIPFAIG 362
           LPTVWL+DLR ISY+SAGGV+AT +I +++ +LGT GGIGFH    G+ V W GIPFAIG
Sbjct: 302 LPTVWLKDLRIISYLSAGGVIATALIAVSVFFLGTTGGIGFH--HTGQAVKWNGIPFAIG 361

Query: 363 AYGFCFSGHTLFPNLYHSMADKTKFTKALLICFVFCVLIYGGIAVMGFFMFGQSILSQIT 422
            YGFC+SGH++FPN+Y SMADKTKF KA++ CF+ CVL+YGG+A+MG+ MFG++ LSQIT
Sbjct: 362 IYGFCYSGHSVFPNIYQSMADKTKFNKAVITCFIICVLLYGGVAIMGYLMFGEATLSQIT 414

Query: 423 LNMPQHALASNVAKWTT 434
           LNMPQ    S VA+WTT
Sbjct: 422 LNMPQDQFFSKVAQWTT 414

BLAST of Tan0003503 vs. TAIR 10
Match: AT2G39130.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 308.1 bits (788), Expect = 1.4e-83
Identity = 178/447 (39.82%), Postives = 255/447 (57.05%), Query Frame = 0

Query: 7   NHRDGDSMVFFQPQQEEEEDDIEMNNKGLESSDGSSGSFSGGYYSAAAVTTAVSGFLRQG 66
           NH   D   + + + E++  D       +E   GS    S   Y              Q 
Sbjct: 2   NHVPSDQSFYIESEDEDDRKDY------VEEDGGSHSDSSDDVYDE-----------NQA 61

Query: 67  YNSASS-SQQWPRSFREASDSYAITTSPTFGILRIPSIIK--SSF----------QNGIE 126
           +   SS +  WP+S+R++ D Y+   SP  G L   S+ +  SSF             + 
Sbjct: 62  HIKPSSYTTAWPQSYRQSIDLYSSVPSPGIGFLGNNSMTRFGSSFLSSGLIRRHTPESLP 121

Query: 127 SVLEFDVDADVDADVAAK-----APLLTNYYGHEGHGLDYRISS--SQQLPTPHGCSFTQ 186
           +V +  ++   D     K       LL+      G       SS  S ++P     S+ Q
Sbjct: 122 TVTKPLLEEQADEQALPKHRLSSQGLLSPIPSRRGSMRKDEKSSMVSHEIPMSRNSSYGQ 181

Query: 187 TIFNGMNFMAGVGLLSTPYTVKEAGWGSLGVLLVFAFVCYFTAMLMKFCFQKTGHFKIMT 246
            + NG+N + GVG+LSTPY  KE GW  L +L V+  + ++T +L+++C       +  T
Sbjct: 182 AVLNGLNVLCGVGILSTPYAAKEGGWLGLMILFVYGLLSFYTGILLRYCLDSESDLE--T 241

Query: 247 FPDLGQAAFGTFGRLFVSILLYLELYCCCIEFIILEEDNLSSLFPNAWLNLGGLHLDSMH 306
           +PD+GQAAFGT GR+FVSI+LYLELY CC+E+IILE DNLSSL+PNA L++GG  LD+ H
Sbjct: 242 YPDIGQAAFGTTGRIFVSIVLYLELYACCVEYIILESDNLSSLYPNAALSIGGFQLDARH 301

Query: 307 LFGIITVLVVLPTVWLRDLRWISYISAGGVLATTVIILTIAYLGTFGGIGFHEVEAGEIV 366
           LF ++T L VLPTVWLRDL  +SYISAGGV+A+ +++L + ++G    +G H    G  +
Sbjct: 302 LFALLTTLAVLPTVWLRDLSVLSYISAGGVIASVLVVLCLFWIGLVDEVGIH--SKGTTL 361

Query: 367 NWKGIPFAIGAYGFCFSGHTLFPNLYHSMADKTKFTKALLICFVFCVLIYGGIAVMGFFM 426
           N   +P AIG YG+C+SGH +FPN+Y SMA  +++   LL CF  C L+Y G+AVMG+ M
Sbjct: 362 NLSTLPVAIGLYGYCYSGHAVFPNIYTSMAKPSQYPAVLLTCFGICTLMYAGVAVMGYTM 421

Query: 427 FGQSILSQITLNMPQHALASNVAKWTT 434
           FG+S  SQ TLN+PQ  +A+ +A WTT
Sbjct: 422 FGESTQSQFTLNLPQDLIATKIAVWTT 427

BLAST of Tan0003503 vs. TAIR 10
Match: AT3G54830.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 293.9 bits (751), Expect = 2.7e-79
Identity = 169/443 (38.15%), Postives = 250/443 (56.43%), Query Frame = 0

Query: 7   NHRDGDSMVFFQPQQEEEEDDIEMNNKGLESSDGSSGSFSGGYYSAAAVTTAVSGFLRQG 66
           NH   D  ++    + ++ DD   +    E  DG+    S  Y       +  S +    
Sbjct: 2   NHSTSDQSLYI---ESDDGDDERKHLSDDEDDDGTLSDTSDAYNQNQHHLSKASPY---- 61

Query: 67  YNSASSSQQWPRSFREASDSYAITTSPTFGILRIPSIIK--SSFQNGIESVLEFDVDADV 126
                 S  WP+S+R++ D +    SP  G L   S+ +  SSF   + S L        
Sbjct: 62  ------STAWPKSYRQSIDMFGSVPSPNLGFLANSSMSRRGSSF---MSSTL-------T 121

Query: 127 DADVAAKAPLLTNYYGHEGHGLDYRISSSQQLPT--------------PHGCSFTQTIFN 186
                   P +T     +     +++S+   LP+               +  SF Q + N
Sbjct: 122 RRHTPESLPCVTKPLLEDEEAPKHKLSTHSLLPSKPSSSMVVSHDMGISNDSSFGQAVLN 181

Query: 187 GMNFMAGVGLLSTPYTVKEAGWGSLGVLLVFAFVCYFTAMLMKFCFQKTGHFKIMTFPDL 246
           G+N + GVG+LSTPY VKE GW  L +L  F  +C++T +L+++C     H  + T+PD+
Sbjct: 182 GVNVLCGVGILSTPYAVKEGGWLGLIILFAFGILCFYTGLLLRYCLD--SHPDVQTYPDI 241

Query: 247 GQAAFGTFGRLFVSILLYLELYCCCIEFIILEEDNLSSLFPNAWLNLGGLHLDSMHLFGI 306
           G AAFG+ GR+ VS++LY+ELY   +E+IILE DNLSS+FPNA L++GG HLD+  LF +
Sbjct: 242 GHAAFGSTGRILVSVILYMELYAMSVEYIILEGDNLSSMFPNASLSIGGFHLDAPRLFAL 301

Query: 307 ITVLVVLPTVWLRDLRWISYISAGGVLATTVIILTIAYLGTFGGIGFHEVEAGEIVNWKG 366
           +T L VLPTVWLRDL  +SYISAGGV+A+ +++L + ++G    +G H    G  +N   
Sbjct: 302 LTTLAVLPTVWLRDLSVLSYISAGGVIASVLVVLCLFWVGLVDDVGIH--SKGTPLNLAT 361

Query: 367 IPFAIGAYGFCFSGHTLFPNLYHSMADKTKFTKALLICFVFCVLIYGGIAVMGFFMFGQS 426
           +P ++G YG+C+SGH +FPN+Y SMA  ++F+  LL  F  C L+Y G+AVMG+ MFG+S
Sbjct: 362 LPVSVGLYGYCYSGHGVFPNIYTSMAKPSQFSAVLLASFGICTLMYAGVAVMGYSMFGES 417

Query: 427 ILSQITLNMPQHALASNVAKWTT 434
             SQ TLN+PQ  +AS +A WTT
Sbjct: 422 TESQFTLNLPQDLVASKIALWTT 417

BLAST of Tan0003503 vs. TAIR 10
Match: AT5G02170.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 285.0 bits (728), Expect = 1.2e-76
Identity = 158/371 (42.59%), Postives = 222/371 (59.84%), Query Frame = 0

Query: 76  WPRSFREASDSYAITTSPT-------FGILRIPSIIKSSFQNGIESVLEFDVDADVDADV 135
           WPRS+R++ D     T PT       F   R  S+  S   +  +  L  D D    + V
Sbjct: 48  WPRSYRQSMDLLTGVTPPTSTSFVSSFRQRRQSSVFGSFTSSPSKQQLLIDKDEIQSSVV 107

Query: 136 AAKAPLLTNYYGHEGHGLDYRISSSQQLPTP---HGCSFTQTIFNGMNFMAGVGLLSTPY 195
           ++    L ++          ++S    L TP     C+F+Q++ NG+N + GV LL+ PY
Sbjct: 108 SSIKSFLASH---------LQLSVPGDLLTPQENRSCTFSQSVLNGINVLCGVALLTMPY 167

Query: 196 TVKEAGWGSLGVLLVFAFVCYFTAMLMKFCFQKTGHFKIMTFPDLGQAAFGTFGRLFVSI 255
            VKE GW  L +L  F  + ++T +L+K C + +    I T+PD+GQAAFGT GR+ VSI
Sbjct: 168 AVKEGGWLGLFILFSFGIITFYTGILLKRCLENSP--GIHTYPDIGQAAFGTTGRILVSI 227

Query: 256 LLYLELYCCCIEFIILEEDNLSSLFPNAWLNLGGLHLDSMHLFGIITVLVVLPTVWLRDL 315
           LLY+ELY  C+E+II+  DNLS +FPN  L + G  LDS  +F I T L+VLPTVWL+DL
Sbjct: 228 LLYVELYASCVEYIIMMSDNLSRMFPNTSLYINGFSLDSTQVFAITTTLIVLPTVWLKDL 287

Query: 316 RWISYISAGGVLATTVIILTIAYLGTFGGIGFHEVEAGEIVNWKGIPFAIGAYGFCFSGH 375
             +SY+SAGGV+++ ++ L + + G+  G+GFH   +G+ ++   IP AIG YGF F  H
Sbjct: 288 SLLSYLSAGGVISSILLALCLFWAGSVDGVGFH--ISGQALDITNIPVAIGIYGFGFGSH 347

Query: 376 TLFPNLYHSMADKTKFTKALLICFVFCVLIYGGIAVMGFFMFGQSILSQITLNMPQHALA 435
           ++FPN+Y SM + +KF   LLI F FC L Y  +AV GF MFG +I SQ TLNMP H  +
Sbjct: 348 SVFPNIYSSMKEPSKFPTVLLISFAFCTLFYIAVAVCGFTMFGDAIQSQFTLNMPPHFTS 405

Query: 436 SNVAKWTTNCT 437
           S +A WT   T
Sbjct: 408 SKIAVWTAVVT 405

BLAST of Tan0003503 vs. TAIR 10
Match: AT3G09330.1 (Transmembrane amino acid transporter family protein )

HSP 1 Score: 284.6 bits (727), Expect = 1.6e-76
Identity = 157/370 (42.43%), Postives = 228/370 (61.62%), Query Frame = 0

Query: 69  SASSSQQWPRSFREASDSYAITTSPTFGILRIPSIIKSSFQNGIESVLEFDVD--ADVDA 128
           S + S  WPRS+R++ D     T PT   +   S  ++SF + + S+ +      A+  A
Sbjct: 39  SQNDSAPWPRSYRQSVDILTGVTPPTISFIHRRS-SQTSFTSSVASLYKRRPTSIANSFA 98

Query: 129 DVAAKAPLLTN----YYGHEGHGLDYRISSSQQLPTPHGCSFTQTIFNGMNFMAGVGLLS 188
              +K PLL+      +     GL    ++      P+ CSF Q++ NG+N + G+ LL+
Sbjct: 99  SSTSKQPLLSEKDDVLFLSSQIGLS---NTDLSYGEPNFCSFPQSVLNGINVLCGISLLT 158

Query: 189 TPYTVKEAGWGSLGVLLVFAFVCYFTAMLMKFCFQKTGHFKIMTFPDLGQAAFGTFGRLF 248
            PY VKE GW  L +LL FA +  +T +L+K C + +   +  T+PD+GQAAFG  GRL 
Sbjct: 159 MPYAVKEGGWLGLCILLSFAIITCYTGILLKRCLESSSDLR--TYPDIGQAAFGFTGRLI 218

Query: 249 VSILLYLELYCCCIEFIILEEDNLSSLFPNAWLNLGGLHLDSMHLFGIITVLVVLPTVWL 308
           +SILLY+ELY CC+E+II+  DNLS +FPN  LN+ G+ LDS  +F I   L+VLPTVWL
Sbjct: 219 ISILLYMELYVCCVEYIIMMSDNLSRVFPNITLNIVGVSLDSPQIFAISATLIVLPTVWL 278

Query: 309 RDLRWISYISAGGVLATTVIILTIAYLGTFGGIGFHEVEAGEIVNWKGIPFAIGAYGFCF 368
           +DL  +SY+SAGGV  + ++ L + ++G+  G+GFH    G+ ++   +P AIG +GF F
Sbjct: 279 KDLSLLSYLSAGGVFVSILLALCLFWVGSVDGVGFH--TGGKSLDLANLPVAIGIFGFGF 338

Query: 369 SGHTLFPNLYHSMADKTKFTKALLICFVFCVLIYGGIAVMGFFMFGQSILSQITLNMPQH 428
           SGH + P++Y SM + +KF   LLI F FCV  Y  +A+ G+ MFG++I SQ TLNMPQ 
Sbjct: 339 SGHAVLPSIYSSMKEPSKFPLVLLISFGFCVFFYIVVAICGYSMFGEAIQSQFTLNMPQQ 398

Query: 429 ALASNVAKWT 433
             AS +A WT
Sbjct: 399 YTASKIAVWT 400