Tan0003310 (gene) Snake gourd v1

Overview


Name	Tan0003310
Type	gene
Organism	Trichosanthes anguina (Snake gourd v1)
Description	Calcium-binding EF-hand family protein
Location	LG03: 56249542 .. 56250592 (-)
RNA-Seq Expression	Tan0003310
Synteny	Tan0003310

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTGATATATATTTTAAAATTAGTCCTTAGCGGCGGATTCTTGTTACACTAAAACGAAACTTCCTGGAAGCTGCCATGGCGGATCTCTCGCTCTTCTCCCTTCCATTTAAGTCGGCAATTCTTCATCGCTTTCACCTCCAAATTCTTCTCTCCTTCTCCATTTTCAATTTCTTCATTCTCTTCTCTTCCTTTTGTAATTTCCGCCATGAAAATCCCCAAGCGATTTAGCCCAAAGCGCCTCTTCCGATCCAAGAAGAACCAGTCCGCCGATGTTTCTCGCTGCGATCCGCCGTCCTTCGGCTCCGGTACGTCCTCCTCCTCTTCCTCCTCTGAGAACTTCTTCAAGCCTCACGCCGCCGCTGCCGCCGGTTGCGGAACCCCAACCAGCGTCCTCCCCCCGGACTGGTCCGATTCGAACTCTGACCAGAAGGAAAACGACGGCGTCGTGTCGAGGAAGGAGTTGGAGGCGATTCTCTCCCGGATCGCGTCTGAGGAGGAGGTTGCGATGATGCTGAGCGAAGTCGGTGACGGCGATGGATTTATTCGCCTGGAGGCGTTGATGGCTCGGGTCGGGTCGGGGTTCGAACCGGCTGGGGAGTCGGAGCTCCGGGATACGTTTGAGTTTTTCGACGCCGATCATGACGGGAGGATCACGGCGGAGGAGCTCCACGGCGTTTTTATGTCGACCGGCGACGAGCGGTACACGTTAGAGGATTGCAAGCGTATGATAGCGGATGTGGATGAGAACGGGGATGGGTTCGTGTGCTTCGCGGAATTTGTGCGTATGATGGAGCTGCAGAGATGATTGAATGTACGGCTCTTGATGCTTATGATGAGAAAATGATCAGACGGCTGGGATGTGATGTGATGGATTATGGGATTTTTTTTAATTTTTTTTTGTTTTGAGTGTGTGCTATGTAATAGACACATGATAATTGCTTTGGGATATTATTGTGGCCGGTCCGACCGGATCAATCAATTAAGAATGAAAATAGGGACTCCAATTTATATGGTTATGATTAAATGAAGTAACTGTCAATACTATTTTGCCA

mRNA sequence

TTGATATATATTTTAAAATTAGTCCTTAGCGGCGGATTCTTGTTACACTAAAACGAAACTTCCTGGAAGCTGCCATGGCGGATCTCTCGCTCTTCTCCCTTCCATTTAAGTCGGCAATTCTTCATCGCTTTCACCTCCAAATTCTTCTCTCCTTCTCCATTTTCAATTTCTTCATTCTCTTCTCTTCCTTTTGTAATTTCCGCCATGAAAATCCCCAAGCGATTTAGCCCAAAGCGCCTCTTCCGATCCAAGAAGAACCAGTCCGCCGATGTTTCTCGCTGCGATCCGCCGTCCTTCGGCTCCGGTACGTCCTCCTCCTCTTCCTCCTCTGAGAACTTCTTCAAGCCTCACGCCGCCGCTGCCGCCGGTTGCGGAACCCCAACCAGCGTCCTCCCCCCGGACTGGTCCGATTCGAACTCTGACCAGAAGGAAAACGACGGCGTCGTGTCGAGGAAGGAGTTGGAGGCGATTCTCTCCCGGATCGCGTCTGAGGAGGAGGTTGCGATGATGCTGAGCGAAGTCGGTGACGGCGATGGATTTATTCGCCTGGAGGCGTTGATGGCTCGGGTCGGGTCGGGGTTCGAACCGGCTGGGGAGTCGGAGCTCCGGGATACGTTTGAGTTTTTCGACGCCGATCATGACGGGAGGATCACGGCGGAGGAGCTCCACGGCGTTTTTATGTCGACCGGCGACGAGCGGTACACGTTAGAGGATTGCAAGCGTATGATAGCGGATGTGGATGAGAACGGGGATGGGTTCGTGTGCTTCGCGGAATTTGTGCGTATGATGGAGCTGCAGAGATGATTGAATGTACGGCTCTTGATGCTTATGATGAGAAAATGATCAGACGGCTGGGATGTGATGTGATGGATTATGGGATTTTTTTTAATTTTTTTTTGTTTTGAGTGTGTGCTATGTAATAGACACATGATAATTGCTTTGGGATATTATTGTGGCCGGTCCGACCGGATCAATCAATTAAGAATGAAAATAGGGACTCCAATTTATATGGTTATGATTAAATGAAGTAACTGTCAATACTATTTTGCCA

Coding sequence (CDS)

ATGAAAATCCCCAAGCGATTTAGCCCAAAGCGCCTCTTCCGATCCAAGAAGAACCAGTCCGCCGATGTTTCTCGCTGCGATCCGCCGTCCTTCGGCTCCGGTACGTCCTCCTCCTCTTCCTCCTCTGAGAACTTCTTCAAGCCTCACGCCGCCGCTGCCGCCGGTTGCGGAACCCCAACCAGCGTCCTCCCCCCGGACTGGTCCGATTCGAACTCTGACCAGAAGGAAAACGACGGCGTCGTGTCGAGGAAGGAGTTGGAGGCGATTCTCTCCCGGATCGCGTCTGAGGAGGAGGTTGCGATGATGCTGAGCGAAGTCGGTGACGGCGATGGATTTATTCGCCTGGAGGCGTTGATGGCTCGGGTCGGGTCGGGGTTCGAACCGGCTGGGGAGTCGGAGCTCCGGGATACGTTTGAGTTTTTCGACGCCGATCATGACGGGAGGATCACGGCGGAGGAGCTCCACGGCGTTTTTATGTCGACCGGCGACGAGCGGTACACGTTAGAGGATTGCAAGCGTATGATAGCGGATGTGGATGAGAACGGGGATGGGTTCGTGTGCTTCGCGGAATTTGTGCGTATGATGGAGCTGCAGAGATGA

Protein sequence

MKIPKRFSPKRLFRSKKNQSADVSRCDPPSFGSGTSSSSSSSENFFKPHAAAAAGCGTPTSVLPPDWSDSNSDQKENDGVVSRKELEAILSRIASEEEVAMMLSEVGDGDGFIRLEALMARVGSGFEPAGESELRDTFEFFDADHDGRITAEELHGVFMSTGDERYTLEDCKRMIADVDENGDGFVCFAEFVRMMELQR

Homology

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BLAST of Tan0003310 vs. ExPASy Swiss-Prot
Match: Q9SS31 (Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 PE=2 SV=1)

HSP 1 Score: 159.5 bits (402), Expect = 4.0e-38
Identity = 110/214 (51.40%), Postives = 141/214 (65.89%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNQSADVSRCDPPSFGSGTSSSSSSSENFFKPHAAAAAGCGTPT 60
           MK+ K   PKR F   K++S  VS+  P +F  G S+SSSS ++          G  TPT
Sbjct: 1   MKLAK-LIPKRFFIRSKDRST-VSK-SPTAFSFG-SASSSSGQDCKNSGGDGGGGSVTPT 60

Query: 61  SVLPPDWSDSNSDQ---------KENDGVVSRKELEAILSRIA----SEEEVAMMLSEVG 120
           S+LP   S  +  +         ++NDG VSR +LE++LSR+     +EEE+ +ML EV 
Sbjct: 61  SILPEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEVD 120

Query: 121 -DGDGFIRLEALMARVGSGFEPAGES-ELRDTFEFFDADHDGRITAEELHGVFMSTGDER 180
            DGDG IRLE L +RV S  +PA +S EL++TFEFFDAD DG I+A+EL  VF + GDER
Sbjct: 121 CDGDGTIRLEELASRVVS-LDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDER 180

Query: 181 YTLEDCKRMIADVDENGDGFVCFAEFVRMMELQR 200
            TL+DCKRMIADVDE+GDGFVCF EF RMM+LQR
Sbjct: 181 CTLDDCKRMIADVDEDGDGFVCFTEFSRMMDLQR 209

[Close]

BLAST of Tan0003310 vs. ExPASy Swiss-Prot
Match: P30188 (Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 PE=2 SV=2)

HSP 1 Score: 150.6 bits (379), Expect = 1.9e-35
Identity = 102/205 (49.76%), Postives = 125/205 (60.98%), Query Frame = 0


Query: 6   RFSPKRLFRSKKNQSADVSRCDPPSFGSGTSSSSSSSENFFKPHAAAAAGCGTPTSVLPP 65
           R SPKRLFR+K    A VSR +P SF S  SSSSS            A    TP SVLP 
Sbjct: 9   RLSPKRLFRTK--SKASVSRSEPSSFSSNASSSSSDGSYGNLKQGPTA----TPISVLPQ 68

Query: 66  DWSDSNSD--------QKENDGVVSRKELEAILSRIA----SEEEVAMMLSEVGDGD-GF 125
           +  D  ++         +++DGVVSR +L A++SR++    S+EEV++ML EV  GD G 
Sbjct: 69  NSGDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGGDGGC 128

Query: 126 IRLEALMARVG--SGFEPAGESELRDTFEFFDADHDGRITAEELHGVFMSTGDERYTLED 185
           I LE L +RV   SG       ELR+ FE FD D +G+I+AEELH VF   GDER TLE+
Sbjct: 129 ISLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERCTLEE 188

Query: 186 CKRMIADVDENGDGFVCFAEFVRMM 196
           C RMIA VD NGDGFVCF +F RMM
Sbjct: 189 CMRMIATVDGNGDGFVCFDDFCRMM 207

[Close]

BLAST of Tan0003310 vs. ExPASy Swiss-Prot
Match: Q9M7R0 (Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 3.0e-17
Identity = 52/133 (39.10%), Postives = 75/133 (56.39%), Query Frame = 0


Query: 71  NSDQKENDGVVSRKELEAILSRI---ASEEEVAMMLSEVG-DGDGFIRLEALMARVGSGF 130
           N      DG +S  EL  +L  +    S+EE+  ++ E+  D DGFI ++   A V +  
Sbjct: 26  NRFDANGDGKISGDELAGVLKALGSNTSKEEIGRIMEEIDTDKDGFINVQEFAAFVKAET 85

Query: 131 EP----AGESELRDTFEFFDADHDGRITAEELHGVFMSTGDERYTLEDCKRMIADVDENG 190
           +P     GE+EL++ FE +D DH+G I++ ELH +    G ERY   DC  MI  VD +G
Sbjct: 86  DPYPSSGGENELKEAFELYDQDHNGLISSVELHKILTRLG-ERYAEHDCVEMIKSVDSDG 145

Query: 191 DGFVCFAEFVRMM 196
           DG+V F EF +MM
Sbjct: 146 DGYVSFEEFKKMM 157

[Close]

BLAST of Tan0003310 vs. ExPASy Swiss-Prot
Match: Q8RZB5 (Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 GN=CML10 PE=2 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 3.9e-17
Identity = 64/157 (40.76%), Postives = 87/157 (55.41%), Query Frame = 0


Query: 48  PHAAAAAGCGTPTSVLPPDWSDSNSDQK---ENDGVVSRKELEAI---LSRIASEEEVAM 107
           P A  A+G G+P     P+       +K     DG +SR EL A+   L   A+++E+A 
Sbjct: 22  PQADPASGGGSPAPT--PEEEMERVFRKFDANGDGRISRSELGALFESLGHAATDDELAR 81

Query: 108 MLSEV-GDGDGFIRLE--ALMARVGSGFEPAGESELRDTFEFFDADHDGRITAEELHGVF 167
           M++E   DGDGFI L+  A +    SG   A E +LR  F  FDAD +G I+A EL  V 
Sbjct: 82  MMAEADADGDGFISLDEFAALNATASGDAAAVEEDLRHAFRVFDADGNGTISAAELARVL 141

Query: 168 MSTGDERYTLEDCKRMIADVDENGDGFVCFAEFVRMM 196
              G E+ T++ C+RMI  VD+NGDG + F EF  MM
Sbjct: 142 HGLG-EKATVQQCRRMIEGVDQNGDGLISFEEFKVMM 175

[Close]

BLAST of Tan0003310 vs. ExPASy Swiss-Prot
Match: Q9C8Y1 (Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 PE=2 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.5e-16
Identity = 54/128 (42.19%), Postives = 77/128 (60.16%), Query Frame = 0


Query: 75  KENDGVVSRKELEAI---LSRIASEEEVAMMLSEVG-DGDGFIRLEALMARVGSGFEPAG 134
           K NDG +S  EL+ +   LS  AS+EE   M+ E   DG+GFI L+  +A      + + 
Sbjct: 25  KNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVALFQISDQSSN 84

Query: 135 ESELRD---TFEFFDADHDGRITAEELHGVFMSTGDERYTLEDCKRMIADVDENGDGFVC 194
            S +RD    F+ +D D +GRI+A ELH V  + G E+ +++DC+RMI  VD +GDG V 
Sbjct: 85  NSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLG-EKCSIQDCQRMINKVDSDGDGCVD 144

Query: 195 FAEFVRMM 196
           F EF +MM
Sbjct: 145 FEEFKKMM 151

[Close]

BLAST of Tan0003310 vs. NCBI nr
Match: XP_022932888.1 (probable calcium-binding protein CML36 [Cucurbita moschata] >KAG6603330.1 putative calcium-binding protein CML36, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 306.6 bits (784), Expect = 1.6e-79
Identity = 167/199 (83.92%), Postives = 176/199 (88.44%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNQSADVSRCDPPSFGSGTSSSSSSSENFFKPHAAAAAGCGTPT 60
           MKIPKRFSPKRLFRSKKN SA+VSR DPPSFGSGTSSSSSSSENFFKPH    AG GTPT
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPH---TAGYGTPT 60

Query: 61  SVLPPDWSDSNSDQKENDGVVSRKELEAILSRIASEEEVAMMLSEVGDGDGFIRLEALMA 120
           SVL    SD  SDQ+ENDGVVSRKELEAILSRIASEEEVAMMLSEV DG GFIRL+ LMA
Sbjct: 61  SVLLAGCSD--SDQRENDGVVSRKELEAILSRIASEEEVAMMLSEV-DGGGFIRLDELMA 120

Query: 121 RVGSGFEPAGESELRDTFEFFDADHDGRITAEELHGVFMSTGDERYTLEDCKRMIADVDE 180
           RVGSGF+  GESELR+TFEFFDADHDGRI+AEELHGV+ S GDER TLEDC+RMI DVDE
Sbjct: 121 RVGSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDE 180

Query: 181 NGDGFVCFAEFVRMMELQR 200
           +GDGFVCF EFVRMMELQR
Sbjct: 181 DGDGFVCFTEFVRMMELQR 193

[Close]

BLAST of Tan0003310 vs. NCBI nr
Match: XP_023543601.1 (probable calcium-binding protein CML36 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 305.8 bits (782), Expect = 2.7e-79
Identity = 167/199 (83.92%), Postives = 175/199 (87.94%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNQSADVSRCDPPSFGSGTSSSSSSSENFFKPHAAAAAGCGTPT 60
           MKIPKRFSPKRLFRSKKN SADVSR DPPSFGSGTSSSSSSSENFFKPH    AG GTPT
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPH---TAGYGTPT 60

Query: 61  SVLPPDWSDSNSDQKENDGVVSRKELEAILSRIASEEEVAMMLSEVGDGDGFIRLEALMA 120
           SVL    SD  SDQ+ENDGVVSRKELEAILSRIASEEEVAMMLSEV DG GFIRL+ LMA
Sbjct: 61  SVLLAGCSD--SDQRENDGVVSRKELEAILSRIASEEEVAMMLSEV-DGGGFIRLDELMA 120

Query: 121 RVGSGFEPAGESELRDTFEFFDADHDGRITAEELHGVFMSTGDERYTLEDCKRMIADVDE 180
           RVGSGF+   ESELR+TFEFFDADHDGRI+AEELHGV+ S GDER TLEDC+RMI DVDE
Sbjct: 121 RVGSGFDSVAESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDE 180

Query: 181 NGDGFVCFAEFVRMMELQR 200
           +GDGFVCF EFVRMMELQR
Sbjct: 181 DGDGFVCFTEFVRMMELQR 193

[Close]

BLAST of Tan0003310 vs. NCBI nr
Match: XP_022967907.1 (probable calcium-binding protein CML36 [Cucurbita maxima])

HSP 1 Score: 303.5 bits (776), Expect = 1.3e-78
Identity = 166/199 (83.42%), Postives = 175/199 (87.94%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNQSADVSRCDPPSFGSGTSSSSSSSENFFKPHAAAAAGCGTPT 60
           MKIPKRFSPKRLFRSKKN SADVSR DPPSFGSGTSSSSSSSENFFKPH    AG GTPT
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPH---TAGYGTPT 60

Query: 61  SVLPPDWSDSNSDQKENDGVVSRKELEAILSRIASEEEVAMMLSEVGDGDGFIRLEALMA 120
           SVL      S+SDQ+END VVSRKELEAILSRIASEEEVAMMLSEV DG GFIRL+ LMA
Sbjct: 61  SVLA---GCSDSDQRENDVVVSRKELEAILSRIASEEEVAMMLSEVDDG-GFIRLDELMA 120

Query: 121 RVGSGFEPAGESELRDTFEFFDADHDGRITAEELHGVFMSTGDERYTLEDCKRMIADVDE 180
           RVGSGF+  GESELR+TFEFFDADHDGRI+AEELHGV+ S GDER TLEDC+RMI DVDE
Sbjct: 121 RVGSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDE 180

Query: 181 NGDGFVCFAEFVRMMELQR 200
           +GDGFVCF EFVRMMELQR
Sbjct: 181 DGDGFVCFTEFVRMMELQR 192

[Close]

BLAST of Tan0003310 vs. NCBI nr
Match: XP_038883626.1 (probable calcium-binding protein CML35 [Benincasa hispida])

HSP 1 Score: 303.1 bits (775), Expect = 1.7e-78
Identity = 167/202 (82.67%), Postives = 174/202 (86.14%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNQSADVSRCDPPSFGSGTSSSSSSSENFFKPHAAAAAGCGTPT 60
           MKIPKRFSPKRLFRSKK  SADVSR DPPSFGSGTSSSSSSSE FFKP+ A A G GTPT
Sbjct: 1   MKIPKRFSPKRLFRSKKYHSADVSRSDPPSFGSGTSSSSSSSETFFKPN-ATATGYGTPT 60

Query: 61  SVLPPDWSDSNSDQKENDGVVSRKELEAILSRIASEEEVAMMLSEV----GDGDGFIRLE 120
           SVLPPDW DS   Q+ENDGVVSRKELEAILSRIASEEEVAMMLSEV    G GDGFI LE
Sbjct: 61  SVLPPDWPDSG--QRENDGVVSRKELEAILSRIASEEEVAMMLSEVSSGGGGGDGFIWLE 120

Query: 121 ALMARVGSGFEPAGESELRDTFEFFDADHDGRITAEELHGVFMSTGDERYTLEDCKRMIA 180
            LMA+VG+G E AGESELR+TFEFFDAD DGRITAEELH VF S GDE+ TLEDC+RMI 
Sbjct: 121 ELMAQVGTGVEAAGESELRETFEFFDADQDGRITAEELHEVFRSIGDEQCTLEDCRRMIR 180

Query: 181 DVDENGDGFVCFAEFVRMMELQ 199
           DVDENGDG VCFAEFVRMMELQ
Sbjct: 181 DVDENGDGVVCFAEFVRMMELQ 199

[Close]

BLAST of Tan0003310 vs. NCBI nr
Match: XP_008457589.1 (PREDICTED: probable calcium-binding protein CML36 [Cucumis melo] >KAA0062715.1 putative calcium-binding protein CML36 [Cucumis melo var. makuwa])

HSP 1 Score: 295.4 bits (755), Expect = 3.6e-76
Identity = 165/200 (82.50%), Postives = 175/200 (87.50%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNQSADVSRCDPPSFGSGTSSSSSSSENFFKPHAAAAAGCGTPT 60
           MKIPKRFSPKRLFRSKK+QSADVSR DPPSFGSGTSSSSSSSENFFKP+ +   G GTPT
Sbjct: 2   MKIPKRFSPKRLFRSKKHQSADVSRSDPPSFGSGTSSSSSSSENFFKPN-SVNTGYGTPT 61

Query: 61  SVLPPDWSDSNSDQKENDGVVSRKELEAILSRIASEEEVAMMLSEV-GDGDGFIRLEALM 120
           SVL       +SD +EN+GVV+RKELEAILSRIASEEEVAMMLSEV   GDGFIRLE LM
Sbjct: 62  SVL-------DSDVRENEGVVTRKELEAILSRIASEEEVAMMLSEVDSGGDGFIRLEELM 121

Query: 121 ARVGSGFEPAGESELRDTFEFFDADHDGRITAEELHGVFMSTGDERYTLEDCKRMIADVD 180
           ARVGSG E AGESELR+TFEFFDAD DGRITAEELHGVF S GDE+ TLEDC+RMI DVD
Sbjct: 122 ARVGSG-EAAGESELRETFEFFDADQDGRITAEELHGVFRSIGDEQCTLEDCRRMIRDVD 181

Query: 181 ENGDGFVCFAEFVRMMELQR 200
           ENGDGFVCFAEFVRMMELQR
Sbjct: 182 ENGDGFVCFAEFVRMMELQR 192

[Close]

BLAST of Tan0003310 vs. ExPASy TrEMBL
Match: A0A6J1F310 (probable calcium-binding protein CML36 OS=Cucurbita moschata OX=3662 GN=LOC111439413 PE=4 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 7.6e-80
Identity = 167/199 (83.92%), Postives = 176/199 (88.44%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNQSADVSRCDPPSFGSGTSSSSSSSENFFKPHAAAAAGCGTPT 60
           MKIPKRFSPKRLFRSKKN SA+VSR DPPSFGSGTSSSSSSSENFFKPH    AG GTPT
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSAEVSRSDPPSFGSGTSSSSSSSENFFKPH---TAGYGTPT 60

Query: 61  SVLPPDWSDSNSDQKENDGVVSRKELEAILSRIASEEEVAMMLSEVGDGDGFIRLEALMA 120
           SVL    SD  SDQ+ENDGVVSRKELEAILSRIASEEEVAMMLSEV DG GFIRL+ LMA
Sbjct: 61  SVLLAGCSD--SDQRENDGVVSRKELEAILSRIASEEEVAMMLSEV-DGGGFIRLDELMA 120

Query: 121 RVGSGFEPAGESELRDTFEFFDADHDGRITAEELHGVFMSTGDERYTLEDCKRMIADVDE 180
           RVGSGF+  GESELR+TFEFFDADHDGRI+AEELHGV+ S GDER TLEDC+RMI DVDE
Sbjct: 121 RVGSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDE 180

Query: 181 NGDGFVCFAEFVRMMELQR 200
           +GDGFVCF EFVRMMELQR
Sbjct: 181 DGDGFVCFTEFVRMMELQR 193

[Close]

BLAST of Tan0003310 vs. ExPASy TrEMBL
Match: A0A6J1HS37 (probable calcium-binding protein CML36 OS=Cucurbita maxima OX=3661 GN=LOC111467281 PE=4 SV=1)

HSP 1 Score: 303.5 bits (776), Expect = 6.4e-79
Identity = 166/199 (83.42%), Postives = 175/199 (87.94%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNQSADVSRCDPPSFGSGTSSSSSSSENFFKPHAAAAAGCGTPT 60
           MKIPKRFSPKRLFRSKKN SADVSR DPPSFGSGTSSSSSSSENFFKPH    AG GTPT
Sbjct: 1   MKIPKRFSPKRLFRSKKNPSADVSRSDPPSFGSGTSSSSSSSENFFKPH---TAGYGTPT 60

Query: 61  SVLPPDWSDSNSDQKENDGVVSRKELEAILSRIASEEEVAMMLSEVGDGDGFIRLEALMA 120
           SVL      S+SDQ+END VVSRKELEAILSRIASEEEVAMMLSEV DG GFIRL+ LMA
Sbjct: 61  SVLA---GCSDSDQRENDVVVSRKELEAILSRIASEEEVAMMLSEVDDG-GFIRLDELMA 120

Query: 121 RVGSGFEPAGESELRDTFEFFDADHDGRITAEELHGVFMSTGDERYTLEDCKRMIADVDE 180
           RVGSGF+  GESELR+TFEFFDADHDGRI+AEELHGV+ S GDER TLEDC+RMI DVDE
Sbjct: 121 RVGSGFDAVGESELRETFEFFDADHDGRISAEELHGVYRSIGDERCTLEDCRRMIRDVDE 180

Query: 181 NGDGFVCFAEFVRMMELQR 200
           +GDGFVCF EFVRMMELQR
Sbjct: 181 DGDGFVCFTEFVRMMELQR 192

[Close]

BLAST of Tan0003310 vs. ExPASy TrEMBL
Match: A0A5A7VA05 (Putative calcium-binding protein CML36 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold382G00410 PE=4 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 1.7e-76
Identity = 165/200 (82.50%), Postives = 175/200 (87.50%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNQSADVSRCDPPSFGSGTSSSSSSSENFFKPHAAAAAGCGTPT 60
           MKIPKRFSPKRLFRSKK+QSADVSR DPPSFGSGTSSSSSSSENFFKP+ +   G GTPT
Sbjct: 2   MKIPKRFSPKRLFRSKKHQSADVSRSDPPSFGSGTSSSSSSSENFFKPN-SVNTGYGTPT 61

Query: 61  SVLPPDWSDSNSDQKENDGVVSRKELEAILSRIASEEEVAMMLSEV-GDGDGFIRLEALM 120
           SVL       +SD +EN+GVV+RKELEAILSRIASEEEVAMMLSEV   GDGFIRLE LM
Sbjct: 62  SVL-------DSDVRENEGVVTRKELEAILSRIASEEEVAMMLSEVDSGGDGFIRLEELM 121

Query: 121 ARVGSGFEPAGESELRDTFEFFDADHDGRITAEELHGVFMSTGDERYTLEDCKRMIADVD 180
           ARVGSG E AGESELR+TFEFFDAD DGRITAEELHGVF S GDE+ TLEDC+RMI DVD
Sbjct: 122 ARVGSG-EAAGESELRETFEFFDADQDGRITAEELHGVFRSIGDEQCTLEDCRRMIRDVD 181

Query: 181 ENGDGFVCFAEFVRMMELQR 200
           ENGDGFVCFAEFVRMMELQR
Sbjct: 182 ENGDGFVCFAEFVRMMELQR 192

[Close]

BLAST of Tan0003310 vs. ExPASy TrEMBL
Match: A0A1S3C5U3 (probable calcium-binding protein CML36 OS=Cucumis melo OX=3656 GN=LOC103497255 PE=4 SV=1)

HSP 1 Score: 295.4 bits (755), Expect = 1.7e-76
Identity = 165/200 (82.50%), Postives = 175/200 (87.50%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNQSADVSRCDPPSFGSGTSSSSSSSENFFKPHAAAAAGCGTPT 60
           MKIPKRFSPKRLFRSKK+QSADVSR DPPSFGSGTSSSSSSSENFFKP+ +   G GTPT
Sbjct: 2   MKIPKRFSPKRLFRSKKHQSADVSRSDPPSFGSGTSSSSSSSENFFKPN-SVNTGYGTPT 61

Query: 61  SVLPPDWSDSNSDQKENDGVVSRKELEAILSRIASEEEVAMMLSEV-GDGDGFIRLEALM 120
           SVL       +SD +EN+GVV+RKELEAILSRIASEEEVAMMLSEV   GDGFIRLE LM
Sbjct: 62  SVL-------DSDVRENEGVVTRKELEAILSRIASEEEVAMMLSEVDSGGDGFIRLEELM 121

Query: 121 ARVGSGFEPAGESELRDTFEFFDADHDGRITAEELHGVFMSTGDERYTLEDCKRMIADVD 180
           ARVGSG E AGESELR+TFEFFDAD DGRITAEELHGVF S GDE+ TLEDC+RMI DVD
Sbjct: 122 ARVGSG-EAAGESELRETFEFFDADQDGRITAEELHGVFRSIGDEQCTLEDCRRMIRDVD 181

Query: 181 ENGDGFVCFAEFVRMMELQR 200
           ENGDGFVCFAEFVRMMELQR
Sbjct: 182 ENGDGFVCFAEFVRMMELQR 192

[Close]

BLAST of Tan0003310 vs. ExPASy TrEMBL
Match: A0A6J1DI26 (probable calcium-binding protein CML36 OS=Momordica charantia OX=3673 GN=LOC111021283 PE=4 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 1.1e-73
Identity = 165/204 (80.88%), Postives = 173/204 (84.80%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNQSADVS-RCDPPSFGSGTSSSSSSSENFFKPH-AAAAAGCGT 60
           MKIPKRFSPKRLFRSKKN+ ADVS R DPPSFGS TSSSSSSSENFFKP  AA+A G GT
Sbjct: 1   MKIPKRFSPKRLFRSKKNR-ADVSRRSDPPSFGSATSSSSSSSENFFKPQTAASAGGYGT 60

Query: 61  PTSVLPPDWSDSNSDQKENDGVVSRKELEAILSRIA----SEEEVAMMLSEVGDGDGFIR 120
           PTSVLP    DS +D  EN+GVVSRKELEAIL RI     SEEEVAMML E  DGDGFIR
Sbjct: 61  PTSVLP----DSAADWSENNGVVSRKELEAILHRIGADPPSEEEVAMMLGEF-DGDGFIR 120

Query: 121 LEALMARVGSGFEPAGESELRDTFEFFDADHDGRITAEELHGVFMSTGDERYTLEDCKRM 180
           LEALM RVGSGF PA E+ELR+ FEFFDADHDGRITAEELHGVF S GDER TLEDC+RM
Sbjct: 121 LEALMDRVGSGFGPACEAELREAFEFFDADHDGRITAEELHGVFSSIGDERCTLEDCRRM 180

Query: 181 IADVDENGDGFVCFAEFVRMMELQ 199
           IADVDENGDGFVCF+EFVRMMELQ
Sbjct: 181 IADVDENGDGFVCFSEFVRMMELQ 198

[Close]

BLAST of Tan0003310 vs. TAIR 10
Match: AT3G10190.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 159.5 bits (402), Expect = 2.9e-39
Identity = 110/214 (51.40%), Postives = 141/214 (65.89%), Query Frame = 0


Query: 1   MKIPKRFSPKRLFRSKKNQSADVSRCDPPSFGSGTSSSSSSSENFFKPHAAAAAGCGTPT 60
           MK+ K   PKR F   K++S  VS+  P +F  G S+SSSS ++          G  TPT
Sbjct: 1   MKLAK-LIPKRFFIRSKDRST-VSK-SPTAFSFG-SASSSSGQDCKNSGGDGGGGSVTPT 60

Query: 61  SVLPPDWSDSNSDQ---------KENDGVVSRKELEAILSRIA----SEEEVAMMLSEVG 120
           S+LP   S  +  +         ++NDG VSR +LE++LSR+     +EEE+ +ML EV 
Sbjct: 61  SILPEVPSPYSYVEILQAFKLIDRDNDGAVSRHDLESLLSRLGPDPLTEEEINVMLKEVD 120

Query: 121 -DGDGFIRLEALMARVGSGFEPAGES-ELRDTFEFFDADHDGRITAEELHGVFMSTGDER 180
            DGDG IRLE L +RV S  +PA +S EL++TFEFFDAD DG I+A+EL  VF + GDER
Sbjct: 121 CDGDGTIRLEELASRVVS-LDPARDSTELKETFEFFDADRDGLISADELLRVFSTIGDER 180

Query: 181 YTLEDCKRMIADVDENGDGFVCFAEFVRMMELQR 200
            TL+DCKRMIADVDE+GDGFVCF EF RMM+LQR
Sbjct: 181 CTLDDCKRMIADVDEDGDGFVCFTEFSRMMDLQR 209

[Close]

BLAST of Tan0003310 vs. TAIR 10
Match: AT2G41410.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 150.6 bits (379), Expect = 1.3e-36
Identity = 102/205 (49.76%), Postives = 125/205 (60.98%), Query Frame = 0


Query: 6   RFSPKRLFRSKKNQSADVSRCDPPSFGSGTSSSSSSSENFFKPHAAAAAGCGTPTSVLPP 65
           R SPKRLFR+K    A VSR +P SF S  SSSSS            A    TP SVLP 
Sbjct: 9   RLSPKRLFRTK--SKASVSRSEPSSFSSNASSSSSDGSYGNLKQGPTA----TPISVLPQ 68

Query: 66  DWSDSNSD--------QKENDGVVSRKELEAILSRIA----SEEEVAMMLSEVGDGD-GF 125
           +  D  ++         +++DGVVSR +L A++SR++    S+EEV++ML EV  GD G 
Sbjct: 69  NSGDFYTELVQAFKLIDRDDDGVVSRGDLAALISRLSHEPPSQEEVSLMLREVDGGDGGC 128

Query: 126 IRLEALMARVG--SGFEPAGESELRDTFEFFDADHDGRITAEELHGVFMSTGDERYTLED 185
           I LE L +RV   SG       ELR+ FE FD D +G+I+AEELH VF   GDER TLE+
Sbjct: 129 ISLEDLASRVAGTSGEGSVETEELREVFEIFDVDRNGKISAEELHRVFGVIGDERCTLEE 188

Query: 186 CKRMIADVDENGDGFVCFAEFVRMM 196
           C RMIA VD NGDGFVCF +F RMM
Sbjct: 189 CMRMIATVDGNGDGFVCFDDFCRMM 207

[Close]

BLAST of Tan0003310 vs. TAIR 10
Match: AT1G66400.1 (calmodulin like 23 )

HSP 1 Score: 87.8 bits (216), Expect = 1.1e-17
Identity = 54/128 (42.19%), Postives = 77/128 (60.16%), Query Frame = 0


Query: 75  KENDGVVSRKELEAI---LSRIASEEEVAMMLSEVG-DGDGFIRLEALMARVGSGFEPAG 134
           K NDG +S  EL+ +   LS  AS+EE   M+ E   DG+GFI L+  +A      + + 
Sbjct: 25  KNNDGKISIDELKDVIGALSPNASQEETKAMMKEFDLDGNGFIDLDEFVALFQISDQSSN 84

Query: 135 ESELRD---TFEFFDADHDGRITAEELHGVFMSTGDERYTLEDCKRMIADVDENGDGFVC 194
            S +RD    F+ +D D +GRI+A ELH V  + G E+ +++DC+RMI  VD +GDG V 
Sbjct: 85  NSAIRDLKEAFDLYDLDRNGRISANELHSVMKNLG-EKCSIQDCQRMINKVDSDGDGCVD 144

Query: 195 FAEFVRMM 196
           F EF +MM
Sbjct: 145 FEEFKKMM 151

[Close]

BLAST of Tan0003310 vs. TAIR 10
Match: AT5G37770.1 (EF hand calcium-binding protein family )

HSP 1 Score: 87.0 bits (214), Expect = 1.8e-17
Identity = 54/130 (41.54%), Postives = 78/130 (60.00%), Query Frame = 0


Query: 75  KENDGVVSRKELEAI---LSRIASEEEVAMMLSEVG-DGDGFIRLEALMARVGSGFEPAG 134
           K  DG +S  EL+ +   LS  AS EE   M+ +   DG+GFI L+  +A    G    G
Sbjct: 27  KNGDGKISVDELKEVIRALSPTASPEETVTMMKQFDLDGNGFIDLDEFVALFQIGIGGGG 86

Query: 135 E-----SELRDTFEFFDADHDGRITAEELHGVFMSTGDERYTLEDCKRMIADVDENGDGF 194
                 S+L++ FE +D D +GRI+A+ELH V  + G E+ +++DCK+MI+ VD +GDG 
Sbjct: 87  NNRNDVSDLKEAFELYDLDGNGRISAKELHSVMKNLG-EKCSVQDCKKMISKVDIDGDGC 146

Query: 195 VCFAEFVRMM 196
           V F EF +MM
Sbjct: 147 VNFDEFKKMM 155

[Close]

BLAST of Tan0003310 vs. TAIR 10
Match: AT3G59440.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 82.0 bits (201), Expect = 5.8e-16
Identity = 53/148 (35.81%), Postives = 80/148 (54.05%), Query Frame = 0


Query: 64  PPDWSDSNSD----------QKENDGVVSRKELEAILSRIA---SEEEVAMMLSEV-GDG 123
           PPD S++ S            K  DG ++++EL   L  +     ++++  M+ ++  +G
Sbjct: 40  PPDESETESPVDLKRVFQMFDKNGDGRITKEELNDSLENLGIFMPDKDLIQMIQKMDANG 99

Query: 124 DGFIRLEALMARVGSGFEPAGESELRDTFEFFDADHDGRITAEELHGVFMSTG-DERYTL 183
           DG + +    +  GS  E   E ++RD F  FD D DG IT EEL+ V  S G  +  TL
Sbjct: 100 DGCVDINEFESLYGSIVEEKEEGDMRDAFNVFDQDGDGFITVEELNSVMTSLGLKQGKTL 159

Query: 184 EDCKRMIADVDENGDGFVCFAEFVRMME 197
           E CK MI  VDE+GDG V + EF++MM+
Sbjct: 160 ECCKEMIMQVDEDGDGRVNYKEFLQMMK 187

The following BLAST results are available for this feature:

BLAST of Tan0003310 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SS31	4.0e-38	51.40	Probable calcium-binding protein CML36 OS=Arabidopsis thaliana OX=3702 GN=CML36 ...	[more]
P30188	1.9e-35	49.76	Probable calcium-binding protein CML35 OS=Arabidopsis thaliana OX=3702 GN=CML35 ...	[more]
Q9M7R0	3.0e-17	39.10	Calcium-binding allergen Ole e 8 OS=Olea europaea OX=4146 PE=1 SV=1	[more]
Q8RZB5	3.9e-17	40.76	Probable calcium-binding protein CML10 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q9C8Y1	1.5e-16	42.19	Probable calcium-binding protein CML23 OS=Arabidopsis thaliana OX=3702 GN=CML23 ...	[more]

BLAST of Tan0003310 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022932888.1	1.6e-79	83.92	probable calcium-binding protein CML36 [Cucurbita moschata] >KAG6603330.1 putati...	[more]
XP_023543601.1	2.7e-79	83.92	probable calcium-binding protein CML36 [Cucurbita pepo subsp. pepo]	[more]
XP_022967907.1	1.3e-78	83.42	probable calcium-binding protein CML36 [Cucurbita maxima]	[more]
XP_038883626.1	1.7e-78	82.67	probable calcium-binding protein CML35 [Benincasa hispida]	[more]
XP_008457589.1	3.6e-76	82.50	PREDICTED: probable calcium-binding protein CML36 [Cucumis melo] >KAA0062715.1 p...	[more]

BLAST of Tan0003310 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F310	7.6e-80	83.92	probable calcium-binding protein CML36 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]
A0A6J1HS37	6.4e-79	83.42	probable calcium-binding protein CML36 OS=Cucurbita maxima OX=3661 GN=LOC1114672...	[more]
A0A5A7VA05	1.7e-76	82.50	Putative calcium-binding protein CML36 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]
A0A1S3C5U3	1.7e-76	82.50	probable calcium-binding protein CML36 OS=Cucumis melo OX=3656 GN=LOC103497255 P...	[more]
A0A6J1DI26	1.1e-73	80.88	probable calcium-binding protein CML36 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]

BLAST of Tan0003310 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G10190.1	2.9e-39	51.40	Calcium-binding EF-hand family protein	[more]
AT2G41410.1	1.3e-36	49.76	Calcium-binding EF-hand family protein	[more]
AT1G66400.1	1.1e-17	42.19	calmodulin like 23	[more]
AT5G37770.1	1.8e-17	41.54	EF hand calcium-binding protein family	[more]
AT3G59440.1	5.8e-16	35.81	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 170..198 e-value: 3.7E-5 score: 33.1 coord: 133..161 e-value: 2.0E-4 score: 30.7
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 132..195 e-value: 3.6E-8 score: 33.7
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 166..199 score: 13.202322
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 129..164 score: 13.816021
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 133..195 e-value: 4.60603E-18 score: 72.5805
None	No IPR available	GENE3D	1.10.238.10		coord: 71..196 e-value: 1.4E-24 score: 88.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..44
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 56..80
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 22..44
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 2..197
None	No IPR available	PANTHER	PTHR10891:SF790	CALCIUM-BINDING PROTEIN CML35-RELATED	coord: 2..197
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 179..191
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 142..154
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 75..195

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Tan0003310.1	Tan0003310.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding