Tan0003287 (gene) Snake gourd v1

Overview
NameTan0003287
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionReplication termination factor 2
LocationLG09: 67318269 .. 67320222 (-)
RNA-Seq ExpressionTan0003287
SyntenyTan0003287
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GGACTTTCGAAGAAACGAAATTTCAGTGCCCTCCCCGTTCATCCCTCGAGTCGGAAAATCGTCGTCTTTCTGATTGTGATCGAAACCCTAGCTATAGAATTTCATCAAGAATCATCGTCGTTCTGATCGTGATCGCGGAACCCTAGCCACGGAATTTTGTCATCGTCTCCGATTCTCAAAACCCTAACCAATGCAACCGAAAAATCAACACCGTCTTCAGATCTTTGTCCATTCTCCCGACCTCCAAATTCAATCGAAAATCGTAAACCTAGCACAAACTTCGGCCCAAACCCTCGAGGACCTTAAGTTCTCTCTTCTCCCCAAAACGCTTGCGCCTTCTTTCTACTTCACAATAAATGGGAAACCTCTCATTGATTCTACGACAATTCCCAGTTCTGTAATTCCCCCTCTGTCCACCTTAATTTTAAGAACCAGGGTCCTTGGGGGCGGTGGCGATGGCGGTGCAACCGGGGCCGAATCCCGTGACTGCTACCTTAATATGTACGCTGAGAAGAAACCTGATAAGGTCGACCCTAATGAGCAGAGGCTGTCCAAGTGGTTGAACTGCGCTCTTTCTAATGAGCCTCTGAGGGAACCTTGTGTGATCGATTGGCTTGGAAACATCTTCAACAAGGAGTCGCTGGTACACGCTTTGCTGGAGAAGAAGCTGCCAAAAGGATTCGGACATATCAAGGGTTTGAAGGATATGGTCAAGATAAATCTTTCGTTGATTCCCGGTACAGAATCGATTGGCAATGCGATTTCGGAGCCGCGGTTCCAATGCCCGATTACTGGGCTCGAGTTCAATGGTAAGTACAAGTTTTTTGCTCTGAGAACTTGTGGACACGTGCTGAGTGCAAAGGCCTTGAAGGAGGTTAAATCTTCTTCCTGCCTTATTTGTCATGCTGAGTTCACGGACAGAGACAAGTTCGTGATCAATGGGAGCGAGGAGGAGGTAGCAGAAATGAGGGAGAGGATGGAGGAAGAGAAATCGAAATTGAAGGAGAAGAAGACGAAGAAAGTGAGGAATGGGGAAGTGGGTATGAATGGAGATGTGAGTGTGGATTTGGCATCATCCCGTTTGTCTGGTAAAAAGCATGGTATTGAAGTTAAGGCTCTGGAGAAGGTTTCGGCTAAGCCTGAAAAGCTTGAGCGGCCAGATAGTGGAGTTCAGGTAAAGGTTGCAGCAAGTAGTGGTGCTGTAAAGAGATTTAAAGCAGCAGACATGGCGCCTGCCAATGCTACCAAAGAAGTTTATGCTTCGATATTCACCTCATCTAAGAAGTCAGATTTTAAGGAAACATTTACTTGTAGATCTCTTCCACTTGGTCGAAACTGATTGGTGTTAAGAACTGCCTAGATCAGTTTATGGTGATTCTCCTTAATATTGTTTGTATCCAAACCTTCATTTTTAGTGTCCTTTTTTAACTTGTTGATTCATGTCTCATCGTTTCTAATTAATGTAAGATATAAGTTTACAGTCATCAACTGCTGTTAATGTTTGGGAGTGATTTTGGAACAATCAAAATCAGTTTTGTCATACTCAGAAGGACTAAAAAAGGTATGACAAAAGTGATTTTGGTCCTTCCAAAATCACACCCAAACATGAACTTACTCTGCTGTGCTGTTTTACATTTTTAGATCAGTCTCGTTGAAAGTTTTGGGCCGATCAATCCATGATCCTCTAGTAGTACAACGTTTGAGTTGATGTTTGAGGTATTGAAAATGAAACCCCTGATAGAGTGCCTAATTTGCATATTTTGACTTCAGATCAAGATCATTTAGAGGGATTCTTTTTGTCAAATATGATTAATAAAGCATACATTGGTATTGGTTATAGTTAGGAGTACCTCTTCTTTGTTGTGACATACATCATCTGTGTCTGCTGAATATTATGTGCTGAGACATCCCATAAATTTTCTGTCTGATGCTTCTGGCGTAGTTTCATCCTGGC

mRNA sequence

GGACTTTCGAAGAAACGAAATTTCAGTGCCCTCCCCGTTCATCCCTCGAGTCGGAAAATCGTCGTCTTTCTGATTGTGATCGAAACCCTAGCTATAGAATTTCATCAAGAATCATCGTCGTTCTGATCGTGATCGCGGAACCCTAGCCACGGAATTTTGTCATCGTCTCCGATTCTCAAAACCCTAACCAATGCAACCGAAAAATCAACACCGTCTTCAGATCTTTGTCCATTCTCCCGACCTCCAAATTCAATCGAAAATCGTAAACCTAGCACAAACTTCGGCCCAAACCCTCGAGGACCTTAAGTTCTCTCTTCTCCCCAAAACGCTTGCGCCTTCTTTCTACTTCACAATAAATGGGAAACCTCTCATTGATTCTACGACAATTCCCAGTTCTGTAATTCCCCCTCTGTCCACCTTAATTTTAAGAACCAGGGTCCTTGGGGGCGGTGGCGATGGCGGTGCAACCGGGGCCGAATCCCGTGACTGCTACCTTAATATGTACGCTGAGAAGAAACCTGATAAGGTCGACCCTAATGAGCAGAGGCTGTCCAAGTGGTTGAACTGCGCTCTTTCTAATGAGCCTCTGAGGGAACCTTGTGTGATCGATTGGCTTGGAAACATCTTCAACAAGGAGTCGCTGGTACACGCTTTGCTGGAGAAGAAGCTGCCAAAAGGATTCGGACATATCAAGGGTTTGAAGGATATGGTCAAGATAAATCTTTCGTTGATTCCCGGTACAGAATCGATTGGCAATGCGATTTCGGAGCCGCGGTTCCAATGCCCGATTACTGGGCTCGAGTTCAATGGTAAGTACAAGTTTTTTGCTCTGAGAACTTGTGGACACGTGCTGAGTGCAAAGGCCTTGAAGGAGGTTAAATCTTCTTCCTGCCTTATTTGTCATGCTGAGTTCACGGACAGAGACAAGTTCGTGATCAATGGGAGCGAGGAGGAGGTAGCAGAAATGAGGGAGAGGATGGAGGAAGAGAAATCGAAATTGAAGGAGAAGAAGACGAAGAAAGTGAGGAATGGGGAAGTGGGTATGAATGGAGATGTGAGTGTGGATTTGGCATCATCCCGTTTGTCTGGTAAAAAGCATGGTATTGAAGTTAAGGCTCTGGAGAAGGTTTCGGCTAAGCCTGAAAAGCTTGAGCGGCCAGATAGTGGAGTTCAGGTAAAGGTTGCAGCAAGTAGTGGTGCTGTAAAGAGATTTAAAGCAGCAGACATGGCGCCTGCCAATGCTACCAAAGAAGTTTATGCTTCGATATTCACCTCATCTAAGAAGTCAGATTTTAAGGAAACATTTACTTGTAGATCTCTTCCACTTGGTCGAAACTGATTGGTGTTAAGAACTGCCTAGATCAGTTTATGGTGATTCTCCTTAATATTGTTTGTATCCAAACCTTCATTTTTAGTGTCCTTTTTTAACTTGTTGATTCATGTCTCATCGTTTCTAATTAATGTAAGATATAAGTTTACAGTCATCAACTGCTGTTAATGTTTGGGAGTGATTTTGGAACAATCAAAATCAGTTTTGTCATACTCAGAAGGACTAAAAAAGGTATGACAAAAGTGATTTTGGTCCTTCCAAAATCACACCCAAACATGAACTTACTCTGCTGTGCTGTTTTACATTTTTAGATCAGTCTCGTTGAAAGTTTTGGGCCGATCAATCCATGATCCTCTAGTAGTACAACGTTTGAGTTGATGTTTGAGGTATTGAAAATGAAACCCCTGATAGAGTGCCTAATTTGCATATTTTGACTTCAGATCAAGATCATTTAGAGGGATTCTTTTTGTCAAATATGATTAATAAAGCATACATTGGTATTGGTTATAGTTAGGAGTACCTCTTCTTTGTTGTGACATACATCATCTGTGTCTGCTGAATATTATGTGCTGAGACATCCCATAAATTTTCTGTCTGATGCTTCTGGCGTAGTTTCATCCTGGC

Coding sequence (CDS)

ATGCAACCGAAAAATCAACACCGTCTTCAGATCTTTGTCCATTCTCCCGACCTCCAAATTCAATCGAAAATCGTAAACCTAGCACAAACTTCGGCCCAAACCCTCGAGGACCTTAAGTTCTCTCTTCTCCCCAAAACGCTTGCGCCTTCTTTCTACTTCACAATAAATGGGAAACCTCTCATTGATTCTACGACAATTCCCAGTTCTGTAATTCCCCCTCTGTCCACCTTAATTTTAAGAACCAGGGTCCTTGGGGGCGGTGGCGATGGCGGTGCAACCGGGGCCGAATCCCGTGACTGCTACCTTAATATGTACGCTGAGAAGAAACCTGATAAGGTCGACCCTAATGAGCAGAGGCTGTCCAAGTGGTTGAACTGCGCTCTTTCTAATGAGCCTCTGAGGGAACCTTGTGTGATCGATTGGCTTGGAAACATCTTCAACAAGGAGTCGCTGGTACACGCTTTGCTGGAGAAGAAGCTGCCAAAAGGATTCGGACATATCAAGGGTTTGAAGGATATGGTCAAGATAAATCTTTCGTTGATTCCCGGTACAGAATCGATTGGCAATGCGATTTCGGAGCCGCGGTTCCAATGCCCGATTACTGGGCTCGAGTTCAATGGTAAGTACAAGTTTTTTGCTCTGAGAACTTGTGGACACGTGCTGAGTGCAAAGGCCTTGAAGGAGGTTAAATCTTCTTCCTGCCTTATTTGTCATGCTGAGTTCACGGACAGAGACAAGTTCGTGATCAATGGGAGCGAGGAGGAGGTAGCAGAAATGAGGGAGAGGATGGAGGAAGAGAAATCGAAATTGAAGGAGAAGAAGACGAAGAAAGTGAGGAATGGGGAAGTGGGTATGAATGGAGATGTGAGTGTGGATTTGGCATCATCCCGTTTGTCTGGTAAAAAGCATGGTATTGAAGTTAAGGCTCTGGAGAAGGTTTCGGCTAAGCCTGAAAAGCTTGAGCGGCCAGATAGTGGAGTTCAGGTAAAGGTTGCAGCAAGTAGTGGTGCTGTAAAGAGATTTAAAGCAGCAGACATGGCGCCTGCCAATGCTACCAAAGAAGTTTATGCTTCGATATTCACCTCATCTAAGAAGTCAGATTTTAAGGAAACATTTACTTGTAGATCTCTTCCACTTGGTCGAAACTGA

Protein sequence

MQPKNQHRLQIFVHSPDLQIQSKIVNLAQTSAQTLEDLKFSLLPKTLAPSFYFTINGKPLIDSTTIPSSVIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPNEQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMVKINLSLIPGTESIGNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLICHAEFTDRDKFVINGSEEEVAEMRERMEEEKSKLKEKKTKKVRNGEVGMNGDVSVDLASSRLSGKKHGIEVKALEKVSAKPEKLERPDSGVQVKVAASSGAVKRFKAADMAPANATKEVYASIFTSSKKSDFKETFTCRSLPLGRN
Homology
BLAST of Tan0003287 vs. ExPASy Swiss-Prot
Match: Q3T1J8 (Replication termination factor 2 OS=Rattus norvegicus OX=10116 GN=Rtf2 PE=2 SV=1)

HSP 1 Score: 140.2 bits (352), Expect = 4.9e-32
Identity = 99/271 (36.53%), Postives = 144/271 (53.14%), Query Frame = 0

Query: 108 KKPDKVDPNEQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFG-- 167
           KK +KVD + + +++W  C LS E LR P V   LG ++NK++++  LL+K   K  G  
Sbjct: 19  KKVEKVDKDAELVAQWNYCTLSQEVLRRPIVACELGRLYNKDAVIEFLLDKSAEKALGKA 78

Query: 168 --HIKGLKDMVKINLSLIPGTE-SIGNA-------ISEPRFQCPITGLEFNGKYKFFALR 227
             HI+ +K++ ++ LS  P  E   GN        +   RF CP+ GLE NG+++F  LR
Sbjct: 79  TSHIRSIKNVTELKLSDNPAWEGDKGNTKGDKHDDLQRARFICPVVGLEMNGRHRFCFLR 138

Query: 228 TCGHVLSAKALKEVKSSSCLICHAEFTDRDKFVINGSEEEVAEMRERMEEEKSKLK-EKK 287
            CG V S +ALKE+K+  C  C A F + D  V+NG++E+V  ++ RMEE + + K EKK
Sbjct: 139 CCGCVFSERALKEIKAEVCHTCGAAFQEEDIIVLNGTKEDVEMLKRRMEERRLRAKLEKK 198

Query: 288 TKKVRNGEVGMNGDVSVDLA--SSRLSGKKHGIEVKALEKVSAKPEKLERPDSGVQVKVA 347
           TKK +  E      +S D A  S   +GK    +    EK S+   +    +     KV 
Sbjct: 199 TKKPKTAESASKLGISQDSAGPSKAKAGKSEEADPDPREKKSSLAPRGTASNGSASGKVG 258

Query: 348 -ASSGAVKRFKAADMAPANATKEVYASIFTS 363
               GA+KR   AD    +   E Y SIFTS
Sbjct: 259 KPPCGALKR-SIAD----SEESETYKSIFTS 284

BLAST of Tan0003287 vs. ExPASy Swiss-Prot
Match: Q5R9P9 (Replication termination factor 2 OS=Pongo abelii OX=9601 GN=RTF2 PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 2.0e-30
Identity = 100/285 (35.09%), Postives = 143/285 (50.18%), Query Frame = 0

Query: 108 KKPDKVDPNEQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFG-- 167
           KK +KVD + + +++W  C LS E LR P V   LG ++NK++++  LL+K   K  G  
Sbjct: 19  KKVEKVDKDAELVAQWNYCTLSQEILRRPIVACELGRLYNKDAVIEFLLDKSAEKALGKA 78

Query: 168 --HIKGLKDMVKINLSLIPGTE-SIGNA-------ISEPRFQCPITGLEFNGKYKFFALR 227
             HIK +K++ ++ LS  P  E   GN        +   RF CP+ GLE NG+++F  LR
Sbjct: 79  ASHIKSIKNVTELKLSDNPAWEGDKGNTKGDKHDDLQRARFICPVVGLEMNGRHRFCFLR 138

Query: 228 TCGHVLSAKALKEVKSSSCLICHAEFTDRDKFVINGSEEEVAEMRERMEEEKSKLK-EKK 287
            CG V S +ALKE+K+  C  C A F + D  V+NG++E+V  ++ RMEE + + K EKK
Sbjct: 139 CCGCVFSERALKEIKAEVCHTCGAAFQEDDVIVLNGTKEDVDVLKTRMEERRLRAKLEKK 198

Query: 288 TKKVRNGEVGMNGDVSVDLASSRLSGKKHGIEVKALEKV-SAKPEKLERPDSGVQVKVAA 347
           TKK +  E     DVS               E     KV + KPE+        +  +A 
Sbjct: 199 TKKPKAAESVSKPDVSE--------------EAPGPSKVKTGKPEEASLDSREKKTNLAP 258

Query: 348 SSGAVKRFKA--ADMAPANATKEVYASIFTSSKKSDFKETFTCRS 377
            S A     +  A   P  ATK    SI  S +   +K  FT  S
Sbjct: 259 KSTATNESSSGKAGKPPCGATKR---SIADSEESEAYKSLFTTHS 286

BLAST of Tan0003287 vs. ExPASy Swiss-Prot
Match: Q99K95 (Replication termination factor 2 OS=Mus musculus OX=10090 GN=Rtf2 PE=1 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 2.7e-30
Identity = 99/272 (36.40%), Postives = 143/272 (52.57%), Query Frame = 0

Query: 108 KKPDKVDPNEQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFG-- 167
           KK +KVD + + +++W  C LS E LR P V   LG ++NK++++  LL+K   K  G  
Sbjct: 19  KKVEKVDKDAELVAQWNYCTLSQEILRRPIVACELGRLYNKDAVIEFLLDKSAEKALGKA 78

Query: 168 --HIKGLKDMVKINLSLIPGTE-SIGNA-------ISEPRFQCPITGLEFNGKYKFFALR 227
             HI+ +K++ ++ LS  P  E   GN        +   RF CP+ GLE NG+++F  LR
Sbjct: 79  ASHIRSIKNVTELRLSDNPAWEGDKGNTKGDKHDDLQRARFICPVVGLEMNGRHRFCFLR 138

Query: 228 TCGHVLSAKALKEVKSSSCLICHAEFTDRDKFVINGSEEEVAEMRERMEEEKSKLK-EKK 287
            CG V S +ALKE+K+  C  C A F + D  V+NG++E+V  +++RMEE + + K EKK
Sbjct: 139 CCGCVFSERALKEIKAEVCHTCGAAFQEEDIIVLNGTKEDVEMLKKRMEERRLRAKLEKK 198

Query: 288 TKKVRNG-EVGMNGDVSVDLA--SSRLSGKKHGIEVKALEKVSAKPEKLERPDSGVQVKV 347
           TKK +   E       + D A  S   SGK    +    EK S    +    +     KV
Sbjct: 199 TKKPKTATECASKPGTTQDSAGPSKVKSGKPEEADPDPREKKSTPAPRGAATNGSASGKV 258

Query: 348 A-ASSGAVKRFKAADMAPANATKEVYASIFTS 363
                GA+KR   AD    +   E Y SIFTS
Sbjct: 259 GKPPCGALKR-SIAD----SEESETYKSIFTS 285

BLAST of Tan0003287 vs. ExPASy Swiss-Prot
Match: Q9BY42 (Replication termination factor 2 OS=Homo sapiens OX=9606 GN=RTF2 PE=1 SV=3)

HSP 1 Score: 134.0 bits (336), Expect = 3.5e-30
Identity = 99/285 (34.74%), Postives = 144/285 (50.53%), Query Frame = 0

Query: 108 KKPDKVDPNEQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFG-- 167
           KK +KVD + + +++W  C LS E LR P V   LG ++NK++++  LL+K   K  G  
Sbjct: 19  KKVEKVDKDAELVAQWNYCTLSQEILRRPIVACELGRLYNKDAVIEFLLDKSAEKALGKA 78

Query: 168 --HIKGLKDMVKINLSLIPGTE-SIGNA-------ISEPRFQCPITGLEFNGKYKFFALR 227
             HIK +K++ ++ LS  P  E   GN        +   RF CP+ GLE NG+++F  LR
Sbjct: 79  ASHIKSIKNVTELKLSDNPAWEGDKGNTKGDKHDDLQRARFICPVVGLEMNGRHRFCFLR 138

Query: 228 TCGHVLSAKALKEVKSSSCLICHAEFTDRDKFVINGSEEEVAEMRERMEEEKSKLK-EKK 287
            CG V S +ALKE+K+  C  C A F + D  ++NG++E+V  ++ RMEE + + K EKK
Sbjct: 139 CCGCVFSERALKEIKAEVCHTCGAAFQEDDVIMLNGTKEDVDVLKTRMEERRLRAKLEKK 198

Query: 288 TKKVRNGEVGMNGDVSVDLASSRLSGKKHGIEVKALEKV-SAKPEKLERPDSGVQVKVAA 347
           TKK +  E     DVS               E     KV + KPE+        +  +A 
Sbjct: 199 TKKPKAAESVSKPDVSE--------------EAPGPSKVKTGKPEEASLDSREKKTNLAP 258

Query: 348 SSGAVKRFKA--ADMAPANATKEVYASIFTSSKKSDFKETFTCRS 377
            S A+    +  A   P  ATK    SI  S +   +K  FT  S
Sbjct: 259 KSTAMNESSSGKAGKPPCGATKR---SIADSEESEAYKSLFTTHS 286

BLAST of Tan0003287 vs. ExPASy Swiss-Prot
Match: Q4R594 (Replication termination factor 2 OS=Macaca fascicularis OX=9541 GN=RTF2 PE=2 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 7.8e-30
Identity = 97/275 (35.27%), Postives = 145/275 (52.73%), Query Frame = 0

Query: 108 KKPDKVDPNEQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFG-- 167
           KK +KVD + + +++W  C LS E LR P V   LG ++NK++++  LL+K   K  G  
Sbjct: 19  KKVEKVDKDAELVAQWNYCTLSQEILRRPIVACELGRLYNKDAVIEFLLDKSAEKALGKA 78

Query: 168 --HIKGLKDMVKINLSLIPGTE-SIGNA-------ISEPRFQCPITGLEFNGKYKFFALR 227
             HIK +K++ ++ LS  P  E   GN        +   RF CP+ GLE NG+++F  LR
Sbjct: 79  ASHIKSIKNVTELKLSDNPAWEGDKGNTKGDKHDDLQRARFICPVVGLEMNGRHRFCFLR 138

Query: 228 TCGHVLSAKALKEVKSSSCLICHAEFTDRDKFVINGSEEEVAEMRERMEEEKSKLK-EKK 287
            CG V S +ALKE+K+  C  C A F + D  V+NG+ E+V  ++ RMEE + + K EKK
Sbjct: 139 CCGCVFSERALKEIKAEVCHTCGAAFQEDDVIVLNGTNEDVDVLKARMEERRLRAKLEKK 198

Query: 288 TKKVRNGEVGMNGDVSVDL--ASSRLSGKKHGIEVKALEKVSAKPEKLERPDSGVQVKVA 347
           TKK +  E     DVS +    S   +GK     + + EK +    K    +     K  
Sbjct: 199 TKKPKAAESVSKPDVSEEAPGPSKVKTGKPEETSLDSREKKNNLAPKSAATNESFSGKAG 258

Query: 348 AS-SGAVKRFKAADMAPANATKEVYASIFTSSKKS 367
               GA KR   AD   + A K ++ +  +S+K+S
Sbjct: 259 KPLCGATKR-SIADSEESEAYKSLFTT-HSSAKRS 291

BLAST of Tan0003287 vs. NCBI nr
Match: XP_038879920.1 (replication termination factor 2 [Benincasa hispida])

HSP 1 Score: 677.2 bits (1746), Expect = 8.4e-191
Identity = 346/388 (89.18%), Postives = 367/388 (94.59%), Query Frame = 0

Query: 1   MQPKNQHRLQIFVHSPDLQIQSKIVNLAQTSAQTLEDLKFSLLPKTL----APSFYFTIN 60
           M PKN+HRLQIF+HS DLQIQS+IVNL Q+ AQTLEDLKFSLLP+TL    A SFYFT+N
Sbjct: 1   MHPKNEHRLQIFLHSLDLQIQSQIVNLPQSPAQTLEDLKFSLLPETLASRVASSFYFTLN 60

Query: 61  GKPLIDSTTIPSSVIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPL DSTTIPSS++PPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN
Sbjct: 61  GKPLPDSTTIPSSLVPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMVKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGN+FNKESLVHALLEKKLPKGFGHIKGLKDM+KI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVHALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 NLSLIPGTESIGNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLI 240
           NLS+IPGTES GNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCL+
Sbjct: 181 NLSVIPGTESRGNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFTDRDKFVINGSEEEVAEMRERMEEEKSK--LKEKKTKKVRNGEVGMNGDVSVDLA 300
           CHAEFT+RDKFVINGSEEEV EMRERMEEEKSK   KEKKTKKVRN E G+NGD+SVD A
Sbjct: 241 CHAEFTNRDKFVINGSEEEVEEMRERMEEEKSKSRSKEKKTKKVRNEEGGINGDMSVDFA 300

Query: 301 SSRLSGKKHGIEVKALEKVSAKPEKLERPDSGVQVKVAASSGAVKRFKAADMAPANATKE 360
           +SRLSGKKHGIEVKALEKVSAKPE+ ERPD GVQVKVAAS+GAVKRFKAADM PANATKE
Sbjct: 301 ASRLSGKKHGIEVKALEKVSAKPERHERPDGGVQVKVAASNGAVKRFKAADMVPANATKE 360

Query: 361 VYASIFTSSKKSDFKETFTCRSLPLGRN 383
           VYASIFTSS+KSDFKET++CRSLPLGRN
Sbjct: 361 VYASIFTSSRKSDFKETYSCRSLPLGRN 388

BLAST of Tan0003287 vs. NCBI nr
Match: XP_022933589.1 (protein RTF2 homolog [Cucurbita moschata])

HSP 1 Score: 676.8 bits (1745), Expect = 1.1e-190
Identity = 346/386 (89.64%), Postives = 361/386 (93.52%), Query Frame = 0

Query: 1   MQPKNQHRLQIFVHSPDLQIQSKIVNLAQTSAQTLEDLKFSLLPKT----LAPSFYFTIN 60
           M PKNQHRLQ FVHSPDLQIQSKIVNLAQTSAQ+L DLK SL+  T    LA SFYFT+N
Sbjct: 1   MYPKNQHRLQFFVHSPDLQIQSKIVNLAQTSAQSLADLKLSLILNTPASRLASSFYFTLN 60

Query: 61  GKPLIDSTTIPSSVIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPL+DS TI SS++PPLSTLILRTRVLGGGGDGGATGAESRDCYL MYAEKKPDKVDPN
Sbjct: 61  GKPLLDSATISSSLVPPLSTLILRTRVLGGGGDGGATGAESRDCYLKMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMVKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKE LVHALLEKKLPKGFGHIKGLKDM+KI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKEPLVHALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 NLSLIPGTESIGNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLI 240
           NLS++PGTES GNAISE RFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCL+
Sbjct: 181 NLSMVPGTESRGNAISEARFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFTDRDKFVINGSEEEVAEMRERMEEEKSKLKEKKTKKVRNGEVGMNGDVSVDLASS 300
           CHAEF DRDKFVINGSEEEV  MRERMEEEK+K KEKKTKKVR GEVGMNGDVSVDLASS
Sbjct: 241 CHAEFMDRDKFVINGSEEEVVRMRERMEEEKTKSKEKKTKKVRTGEVGMNGDVSVDLASS 300

Query: 301 RLSGKKHGIEVKALEKVSAKPEKLERPDSGVQVKVAASSGAVKRFKAADMAPANATKEVY 360
           RLSGKKHGIE KAL KVSAKPEK ERPDSGVQVKVAAS+GAVKRFKAADMAPANATKEVY
Sbjct: 301 RLSGKKHGIEGKALGKVSAKPEKHERPDSGVQVKVAASNGAVKRFKAADMAPANATKEVY 360

Query: 361 ASIFTSSKKSDFKETFTCRSLPLGRN 383
           ASIFTSS+KSDFKET++CRSLPLGRN
Sbjct: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386

BLAST of Tan0003287 vs. NCBI nr
Match: KAG6587836.1 (Replication termination factor 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 672.5 bits (1734), Expect = 2.1e-189
Identity = 345/386 (89.38%), Postives = 359/386 (93.01%), Query Frame = 0

Query: 1   MQPKNQHRLQIFVHSPDLQIQSKIVNLAQTSAQTLEDLKFSLLPKT----LAPSFYFTIN 60
           M PKNQHRLQ FVHSPDLQIQSKIVNLAQTSAQ+L DLK SL+  T    LA SFYFT+N
Sbjct: 1   MYPKNQHRLQFFVHSPDLQIQSKIVNLAQTSAQSLADLKLSLILNTPASRLASSFYFTLN 60

Query: 61  GKPLIDSTTIPSSVIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPL+DS TI SS++PPLSTLILRTRVLGGGGDGGATGAESRDCYL MYAEKKPDKVDPN
Sbjct: 61  GKPLLDSATISSSLVPPLSTLILRTRVLGGGGDGGATGAESRDCYLKMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMVKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKE LVHALLEKKLPKGFGHIKGLKDM+KI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKEPLVHALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 NLSLIPGTESIGNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLI 240
           NLS+IPGTES GNAISE RFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCL+
Sbjct: 181 NLSMIPGTESRGNAISEARFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFTDRDKFVINGSEEEVAEMRERMEEEKSKLKEKKTKKVRNGEVGMNGDVSVDLASS 300
           CHAEF DRDKFVINGSEEEV  MRERMEEEK+K KEKKTKKVR GEVGMNGDVSVDLASS
Sbjct: 241 CHAEFMDRDKFVINGSEEEVVRMRERMEEEKTKSKEKKTKKVRTGEVGMNGDVSVDLASS 300

Query: 301 RLSGKKHGIEVKALEKVSAKPEKLERPDSGVQVKVAASSGAVKRFKAADMAPANATKEVY 360
           RLSGKKHGIE KAL KVSAK EK ERPDSGVQVKVAAS+G VKRFKAADMAPANATKEVY
Sbjct: 301 RLSGKKHGIEGKALGKVSAKSEKHERPDSGVQVKVAASNGGVKRFKAADMAPANATKEVY 360

Query: 361 ASIFTSSKKSDFKETFTCRSLPLGRN 383
           ASIFTSS+KSDFKET++CRSLPLGRN
Sbjct: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386

BLAST of Tan0003287 vs. NCBI nr
Match: KAG7035670.1 (Protein RTF2-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 672.2 bits (1733), Expect = 2.7e-189
Identity = 344/386 (89.12%), Postives = 359/386 (93.01%), Query Frame = 0

Query: 1   MQPKNQHRLQIFVHSPDLQIQSKIVNLAQTSAQTLEDLKFSLLPKT----LAPSFYFTIN 60
           M PKNQHRLQ FVHSPDLQIQSKIVNLAQTSAQ+L DLK SL+  T    LA SFYFT+N
Sbjct: 1   MYPKNQHRLQFFVHSPDLQIQSKIVNLAQTSAQSLADLKLSLILNTPASRLASSFYFTLN 60

Query: 61  GKPLIDSTTIPSSVIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPL+DS TI SS++PPLSTLILRTRVLGGGGDGGATGAESRDCYL MYAEKKPDKVDPN
Sbjct: 61  GKPLLDSATISSSLVPPLSTLILRTRVLGGGGDGGATGAESRDCYLKMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMVKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKE LVHALLEKKLPKGFGH+KGLKDM+KI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKEPLVHALLEKKLPKGFGHVKGLKDMIKI 180

Query: 181 NLSLIPGTESIGNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLI 240
           NLS+IPGTES GNAISE RFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCL+
Sbjct: 181 NLSMIPGTESRGNAISEARFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFTDRDKFVINGSEEEVAEMRERMEEEKSKLKEKKTKKVRNGEVGMNGDVSVDLASS 300
           CHAEF DRDKFVINGSEEEV  MRERMEEEK+K KEKKTKKVR GEVGMNGDVSVDLASS
Sbjct: 241 CHAEFMDRDKFVINGSEEEVVRMRERMEEEKTKSKEKKTKKVRTGEVGMNGDVSVDLASS 300

Query: 301 RLSGKKHGIEVKALEKVSAKPEKLERPDSGVQVKVAASSGAVKRFKAADMAPANATKEVY 360
           RLSGKKHGIE KAL KVSAK EK ERPDSGVQVKVAAS+G VKRFKAADMAPANATKEVY
Sbjct: 301 RLSGKKHGIEGKALGKVSAKSEKHERPDSGVQVKVAASNGGVKRFKAADMAPANATKEVY 360

Query: 361 ASIFTSSKKSDFKETFTCRSLPLGRN 383
           ASIFTSS+KSDFKET++CRSLPLGRN
Sbjct: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386

BLAST of Tan0003287 vs. NCBI nr
Match: XP_023005959.1 (protein RTF2 homolog isoform X1 [Cucurbita maxima] >XP_023005964.1 protein RTF2 homolog isoform X2 [Cucurbita maxima] >XP_023005967.1 protein RTF2 homolog isoform X3 [Cucurbita maxima])

HSP 1 Score: 671.4 bits (1731), Expect = 4.6e-189
Identity = 344/386 (89.12%), Postives = 358/386 (92.75%), Query Frame = 0

Query: 1   MQPKNQHRLQIFVHSPDLQIQSKIVNLAQTSAQTLEDLKFSLL----PKTLAPSFYFTIN 60
           M PKNQHRLQ FV SPDLQIQSKIVNLAQTSAQ+L DLK SLL       LA SFYFT+N
Sbjct: 1   MYPKNQHRLQFFVRSPDLQIQSKIVNLAQTSAQSLADLKLSLLLNAPASRLASSFYFTLN 60

Query: 61  GKPLIDSTTIPSSVIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPL+DS TI SS++PPLSTLILRTRVLGGGGDGGATGAESRDCYL MYAEKKPDKVDPN
Sbjct: 61  GKPLLDSATISSSLVPPLSTLILRTRVLGGGGDGGATGAESRDCYLKMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMVKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDM+KI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 NLSLIPGTESIGNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLI 240
           NLS+IPGTES GN ISE RFQCPITGLEFNGKYKFFALR CGHVLSAKALKEVKSSSCL+
Sbjct: 181 NLSMIPGTESRGNEISEARFQCPITGLEFNGKYKFFALRACGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFTDRDKFVINGSEEEVAEMRERMEEEKSKLKEKKTKKVRNGEVGMNGDVSVDLASS 300
           CHAEF DRDKFVINGSEEEV  MRERMEEEK+K KEKKTKKVR GEVGMNGDVSVDLASS
Sbjct: 241 CHAEFMDRDKFVINGSEEEVVRMRERMEEEKTKAKEKKTKKVRTGEVGMNGDVSVDLASS 300

Query: 301 RLSGKKHGIEVKALEKVSAKPEKLERPDSGVQVKVAASSGAVKRFKAADMAPANATKEVY 360
           RLSGKKHGIE KAL K+SAKPEK ERPDSGVQVKVAAS+GAVKRFKAADMAPANATKEVY
Sbjct: 301 RLSGKKHGIEGKALGKISAKPEKHERPDSGVQVKVAASNGAVKRFKAADMAPANATKEVY 360

Query: 361 ASIFTSSKKSDFKETFTCRSLPLGRN 383
           ASIFTSS+KSDFKET++CRSLPLGRN
Sbjct: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386

BLAST of Tan0003287 vs. ExPASy TrEMBL
Match: A0A6J1F067 (Replication termination factor 2 OS=Cucurbita moschata OX=3662 GN=LOC111440971 PE=3 SV=1)

HSP 1 Score: 676.8 bits (1745), Expect = 5.3e-191
Identity = 346/386 (89.64%), Postives = 361/386 (93.52%), Query Frame = 0

Query: 1   MQPKNQHRLQIFVHSPDLQIQSKIVNLAQTSAQTLEDLKFSLLPKT----LAPSFYFTIN 60
           M PKNQHRLQ FVHSPDLQIQSKIVNLAQTSAQ+L DLK SL+  T    LA SFYFT+N
Sbjct: 1   MYPKNQHRLQFFVHSPDLQIQSKIVNLAQTSAQSLADLKLSLILNTPASRLASSFYFTLN 60

Query: 61  GKPLIDSTTIPSSVIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPL+DS TI SS++PPLSTLILRTRVLGGGGDGGATGAESRDCYL MYAEKKPDKVDPN
Sbjct: 61  GKPLLDSATISSSLVPPLSTLILRTRVLGGGGDGGATGAESRDCYLKMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMVKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKE LVHALLEKKLPKGFGHIKGLKDM+KI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKEPLVHALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 NLSLIPGTESIGNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLI 240
           NLS++PGTES GNAISE RFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCL+
Sbjct: 181 NLSMVPGTESRGNAISEARFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFTDRDKFVINGSEEEVAEMRERMEEEKSKLKEKKTKKVRNGEVGMNGDVSVDLASS 300
           CHAEF DRDKFVINGSEEEV  MRERMEEEK+K KEKKTKKVR GEVGMNGDVSVDLASS
Sbjct: 241 CHAEFMDRDKFVINGSEEEVVRMRERMEEEKTKSKEKKTKKVRTGEVGMNGDVSVDLASS 300

Query: 301 RLSGKKHGIEVKALEKVSAKPEKLERPDSGVQVKVAASSGAVKRFKAADMAPANATKEVY 360
           RLSGKKHGIE KAL KVSAKPEK ERPDSGVQVKVAAS+GAVKRFKAADMAPANATKEVY
Sbjct: 301 RLSGKKHGIEGKALGKVSAKPEKHERPDSGVQVKVAASNGAVKRFKAADMAPANATKEVY 360

Query: 361 ASIFTSSKKSDFKETFTCRSLPLGRN 383
           ASIFTSS+KSDFKET++CRSLPLGRN
Sbjct: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386

BLAST of Tan0003287 vs. ExPASy TrEMBL
Match: A0A6J1KUM3 (Replication termination factor 2 OS=Cucurbita maxima OX=3661 GN=LOC111498815 PE=3 SV=1)

HSP 1 Score: 671.4 bits (1731), Expect = 2.2e-189
Identity = 344/386 (89.12%), Postives = 358/386 (92.75%), Query Frame = 0

Query: 1   MQPKNQHRLQIFVHSPDLQIQSKIVNLAQTSAQTLEDLKFSLL----PKTLAPSFYFTIN 60
           M PKNQHRLQ FV SPDLQIQSKIVNLAQTSAQ+L DLK SLL       LA SFYFT+N
Sbjct: 1   MYPKNQHRLQFFVRSPDLQIQSKIVNLAQTSAQSLADLKLSLLLNAPASRLASSFYFTLN 60

Query: 61  GKPLIDSTTIPSSVIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPL+DS TI SS++PPLSTLILRTRVLGGGGDGGATGAESRDCYL MYAEKKPDKVDPN
Sbjct: 61  GKPLLDSATISSSLVPPLSTLILRTRVLGGGGDGGATGAESRDCYLKMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMVKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDM+KI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 NLSLIPGTESIGNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLI 240
           NLS+IPGTES GN ISE RFQCPITGLEFNGKYKFFALR CGHVLSAKALKEVKSSSCL+
Sbjct: 181 NLSMIPGTESRGNEISEARFQCPITGLEFNGKYKFFALRACGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFTDRDKFVINGSEEEVAEMRERMEEEKSKLKEKKTKKVRNGEVGMNGDVSVDLASS 300
           CHAEF DRDKFVINGSEEEV  MRERMEEEK+K KEKKTKKVR GEVGMNGDVSVDLASS
Sbjct: 241 CHAEFMDRDKFVINGSEEEVVRMRERMEEEKTKAKEKKTKKVRTGEVGMNGDVSVDLASS 300

Query: 301 RLSGKKHGIEVKALEKVSAKPEKLERPDSGVQVKVAASSGAVKRFKAADMAPANATKEVY 360
           RLSGKKHGIE KAL K+SAKPEK ERPDSGVQVKVAAS+GAVKRFKAADMAPANATKEVY
Sbjct: 301 RLSGKKHGIEGKALGKISAKPEKHERPDSGVQVKVAASNGAVKRFKAADMAPANATKEVY 360

Query: 361 ASIFTSSKKSDFKETFTCRSLPLGRN 383
           ASIFTSS+KSDFKET++CRSLPLGRN
Sbjct: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386

BLAST of Tan0003287 vs. ExPASy TrEMBL
Match: A0A6J1C136 (Replication termination factor 2 OS=Momordica charantia OX=3673 GN=LOC111007406 PE=3 SV=1)

HSP 1 Score: 665.2 bits (1715), Expect = 1.6e-187
Identity = 338/386 (87.56%), Postives = 361/386 (93.52%), Query Frame = 0

Query: 1   MQPKNQHRLQIFVHSPDLQIQSKIVNLAQTSAQTLEDLKFSLLPKTLAP----SFYFTIN 60
           M PKNQ RLQIFVHSPDLQIQSKI+NLA+TSAQTLEDLKFSLLP+TLAP    SFYFT+N
Sbjct: 1   MHPKNQQRLQIFVHSPDLQIQSKILNLARTSAQTLEDLKFSLLPQTLAPRSASSFYFTLN 60

Query: 61  GKPLIDSTTIPSSVIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPL+DST + SS+I PLSTLILRTR+ GGGGDGGATGAESRDCYLNMYAEKKPDKVDPN
Sbjct: 61  GKPLLDSTPVTSSLISPLSTLILRTRIHGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMVKI 180
           EQRLSKWLNCALSNEPLREPCVIDWLGN+FNKESLV ALLEKKLPKGFGH+KGLKDM+KI
Sbjct: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNVFNKESLVQALLEKKLPKGFGHVKGLKDMIKI 180

Query: 181 NLSLIPGTESIGNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLI 240
           +LS IPGTES  NAIS PRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCL+
Sbjct: 181 SLSAIPGTESGSNAISGPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFTDRDKFVINGSEEEVAEMRERMEEEKSKLKEKKTKKVRNGEVGMNGDVSVDLASS 300
           CHAEF DRDK VINGSEEEVA +RERMEEEKSK KEK+ KKV+NGEVGMNGDVSVDLASS
Sbjct: 241 CHAEFKDRDKLVINGSEEEVAVLRERMEEEKSKSKEKRLKKVKNGEVGMNGDVSVDLASS 300

Query: 301 RLSGKKHGIEVKALEKVSAKPEKLERPDSGVQVKVAASSGAVKRFKAADMAPANATKEVY 360
           RLSGKKHGIEVKA EKVSAK E+ ERPD G+QVKVAA++GAVKRFKAADMAP NATKEVY
Sbjct: 301 RLSGKKHGIEVKASEKVSAKLERNERPDGGIQVKVAANNGAVKRFKAADMAPPNATKEVY 360

Query: 361 ASIFTSSKKSDFKETFTCRSLPLGRN 383
           ASIFTSS+KSDFKET++CRSLPLGRN
Sbjct: 361 ASIFTSSRKSDFKETYSCRSLPLGRN 386

BLAST of Tan0003287 vs. ExPASy TrEMBL
Match: A0A5A7SWS4 (Replication termination factor 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1369G00470 PE=3 SV=1)

HSP 1 Score: 661.8 bits (1706), Expect = 1.8e-186
Identity = 340/388 (87.63%), Postives = 362/388 (93.30%), Query Frame = 0

Query: 1   MQPKNQHRLQIFVHSPDLQIQSKIVNLAQTSAQTLEDLKFSLLPKTL----APSFYFTIN 60
           M PKNQHRLQIF+HSPDLQIQSKIV+L QTSA+TLEDLKFSLL +TL    A S YFT+N
Sbjct: 1   MHPKNQHRLQIFLHSPDLQIQSKIVDLPQTSAKTLEDLKFSLLSETLASRVASSLYFTLN 60

Query: 61  GKPLIDSTTIPSSVIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPL+DSTTI  S+IPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN
Sbjct: 61  GKPLLDSTTI--SLIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMVKI 180
           EQRLSKWLNCALSNEPL EPCVIDWLGN+FNKESLV ALLEKKLPKGFGHIKGLKDM+KI
Sbjct: 121 EQRLSKWLNCALSNEPLSEPCVIDWLGNVFNKESLVQALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 NLSLIPGTESIGNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLI 240
           N S+IPGTES GNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCL+
Sbjct: 181 NFSMIPGTESRGNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFTDRDKFVINGSEEEVAEMRERMEEE--KSKLKEKKTKKVRNGEVGMNGDVSVDLA 300
           CH EFT+RDKFVINGSEEEV EMRERMEEE  KSK KEKKTKKVRNGEVGMNGDV++DLA
Sbjct: 241 CHTEFTERDKFVINGSEEEVEEMRERMEEEKSKSKSKEKKTKKVRNGEVGMNGDVTMDLA 300

Query: 301 SSRLSGKKHGIEVKALEKVSAKPEKLERPDSGVQVKVAASSGAVKRFKAADMAPANATKE 360
           +SRLSGKKHGIE++ LEKVSAKPE+ ER D G QVKVAAS+GAVKRFKAADM PANATKE
Sbjct: 301 TSRLSGKKHGIELQTLEKVSAKPERHERLDGGAQVKVAASNGAVKRFKAADMVPANATKE 360

Query: 361 VYASIFTSSKKSDFKETFTCRSLPLGRN 383
           VYASIFTSS+KSDFKET++CRSLPLGRN
Sbjct: 361 VYASIFTSSRKSDFKETYSCRSLPLGRN 386

BLAST of Tan0003287 vs. ExPASy TrEMBL
Match: A0A1S3B9F3 (Replication termination factor 2 OS=Cucumis melo OX=3656 GN=LOC103487489 PE=3 SV=1)

HSP 1 Score: 661.8 bits (1706), Expect = 1.8e-186
Identity = 340/388 (87.63%), Postives = 362/388 (93.30%), Query Frame = 0

Query: 1   MQPKNQHRLQIFVHSPDLQIQSKIVNLAQTSAQTLEDLKFSLLPKTL----APSFYFTIN 60
           M PKNQHRLQIF+HSPDLQIQSKIV+L QTSA+TLEDLKFSLL +TL    A S YFT+N
Sbjct: 1   MHPKNQHRLQIFLHSPDLQIQSKIVDLPQTSAKTLEDLKFSLLSETLASRVASSLYFTLN 60

Query: 61  GKPLIDSTTIPSSVIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120
           GKPL+DSTTI  S+IPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN
Sbjct: 61  GKPLLDSTTI--SLIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPN 120

Query: 121 EQRLSKWLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMVKI 180
           EQRLSKWLNCALSNEPL EPCVIDWLGN+FNKESLV ALLEKKLPKGFGHIKGLKDM+KI
Sbjct: 121 EQRLSKWLNCALSNEPLSEPCVIDWLGNVFNKESLVQALLEKKLPKGFGHIKGLKDMIKI 180

Query: 181 NLSLIPGTESIGNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLI 240
           N S+IPGTES GNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCL+
Sbjct: 181 NFSMIPGTESRGNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLV 240

Query: 241 CHAEFTDRDKFVINGSEEEVAEMRERMEEE--KSKLKEKKTKKVRNGEVGMNGDVSVDLA 300
           CH EFT+RDKFVINGSEEEV EMRERMEEE  KSK KEKKTKKVRNGEVGMNGDV++DLA
Sbjct: 241 CHTEFTERDKFVINGSEEEVEEMRERMEEEKSKSKSKEKKTKKVRNGEVGMNGDVTMDLA 300

Query: 301 SSRLSGKKHGIEVKALEKVSAKPEKLERPDSGVQVKVAASSGAVKRFKAADMAPANATKE 360
           +SRLSGKKHGIE++ LEKVSAKPE+ ER D G QVKVAAS+GAVKRFKAADM PANATKE
Sbjct: 301 TSRLSGKKHGIELQTLEKVSAKPERHERLDGGAQVKVAASNGAVKRFKAADMVPANATKE 360

Query: 361 VYASIFTSSKKSDFKETFTCRSLPLGRN 383
           VYASIFTSS+KSDFKET++CRSLPLGRN
Sbjct: 361 VYASIFTSSRKSDFKETYSCRSLPLGRN 386

BLAST of Tan0003287 vs. TAIR 10
Match: AT5G58020.1 (unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF602 (InterPro:IPR006735); Has 385 Blast hits to 385 proteins in 189 species: Archae - 0; Bacteria - 0; Metazoa - 153; Fungi - 117; Plants - 50; Viruses - 0; Other Eukaryotes - 65 (source: NCBI BLink). )

HSP 1 Score: 392.5 bits (1007), Expect = 3.9e-109
Identity = 227/383 (59.27%), Postives = 263/383 (68.67%), Query Frame = 0

Query: 8   RLQIFVHSPDLQIQSKIVNLAQTSAQTLEDLK--FSLLPKTLAPSF---YFTINGKPLID 67
           R QIFV SPD Q   K+V L    AQ+L  L    SLL  +   SF     T++GK L  
Sbjct: 4   RRQIFVKSPDCQ---KVVALQLDPAQSLLTLSGITSLLESSQRISFSACSITLDGKLLNG 63

Query: 68  STTIPSSVIPPLSTLILRTRVLGGGGDGGATGAESRDCYLNMYAEKKPDKVDPNEQRLSK 127
           ST I  S +P +S L L  R+ GGGGDGGATGAESRDCYLNMYAEKKPDKVDPNEQRLSK
Sbjct: 64  STRIQVSKLPSVSMLTLFPRLRGGGGDGGATGAESRDCYLNMYAEKKPDKVDPNEQRLSK 123

Query: 128 WLNCALSNEPLREPCVIDWLGNIFNKESLVHALLEKKLPKGFGHIKGLKDMVKINLSLIP 187
           WLNCALSNEPL EPCVID LGN+FNKE LVHALL K+LPK F +IKGLKDMV I L+ + 
Sbjct: 124 WLNCALSNEPLAEPCVIDLLGNLFNKEVLVHALLSKRLPKQFSYIKGLKDMVNIKLTPVA 183

Query: 188 GTESIGNAISEPRFQCPITGLEFNGKYKFFALRTCGHVLSAKALKEVKSSSCLICHAEFT 247
           G++      +  +FQCP++GLEFNGKYKFFALR CGHV+SAKALKEVKSSSCL+CHA+  
Sbjct: 184 GSDGSSQDTTSAQFQCPVSGLEFNGKYKFFALRGCGHVMSAKALKEVKSSSCLVCHADVK 243

Query: 248 DRDKFVINGSEEEVAEMRERMEEEKSKLKEKK--TKKVRNGEVGMNGDVSVDLASSRLSG 307
           D DK VING+EEEV  +RERMEEEK+KL+EKK  +KK +NG                   
Sbjct: 244 DSDKIVINGTEEEVDLLRERMEEEKAKLREKKGVSKKSKNG------------------- 303

Query: 308 KKHGIEVKALEKVSAKPEKLERPDSGVQVKVAASSGAVKRFKAADMAPANATKEVYASIF 367
                    +    AK  K +  D  V      +   VK+FKAAD  P NATKEVYAS+F
Sbjct: 304 ------AAVVADTGAKVAKRQIEDGNVN----GNGITVKKFKAADKVPVNATKEVYASLF 354

Query: 368 TSS-KKSDFKETFTCRSLPLGRN 383
           TSS KKSDF+ET++CRSLPLGRN
Sbjct: 364 TSSKKKSDFRETYSCRSLPLGRN 354

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3T1J84.9e-3236.53Replication termination factor 2 OS=Rattus norvegicus OX=10116 GN=Rtf2 PE=2 SV=1[more]
Q5R9P92.0e-3035.09Replication termination factor 2 OS=Pongo abelii OX=9601 GN=RTF2 PE=2 SV=1[more]
Q99K952.7e-3036.40Replication termination factor 2 OS=Mus musculus OX=10090 GN=Rtf2 PE=1 SV=1[more]
Q9BY423.5e-3034.74Replication termination factor 2 OS=Homo sapiens OX=9606 GN=RTF2 PE=1 SV=3[more]
Q4R5947.8e-3035.27Replication termination factor 2 OS=Macaca fascicularis OX=9541 GN=RTF2 PE=2 SV=... [more]
Match NameE-valueIdentityDescription
XP_038879920.18.4e-19189.18replication termination factor 2 [Benincasa hispida][more]
XP_022933589.11.1e-19089.64protein RTF2 homolog [Cucurbita moschata][more]
KAG6587836.12.1e-18989.38Replication termination factor 2, partial [Cucurbita argyrosperma subsp. sororia... [more]
KAG7035670.12.7e-18989.12Protein RTF2-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023005959.14.6e-18989.12protein RTF2 homolog isoform X1 [Cucurbita maxima] >XP_023005964.1 protein RTF2 ... [more]
Match NameE-valueIdentityDescription
A0A6J1F0675.3e-19189.64Replication termination factor 2 OS=Cucurbita moschata OX=3662 GN=LOC111440971 P... [more]
A0A6J1KUM32.2e-18989.12Replication termination factor 2 OS=Cucurbita maxima OX=3661 GN=LOC111498815 PE=... [more]
A0A6J1C1361.6e-18787.56Replication termination factor 2 OS=Momordica charantia OX=3673 GN=LOC111007406 ... [more]
A0A5A7SWS41.8e-18687.63Replication termination factor 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
A0A1S3B9F31.8e-18687.63Replication termination factor 2 OS=Cucumis melo OX=3656 GN=LOC103487489 PE=3 SV... [more]
Match NameE-valueIdentityDescription
AT5G58020.13.9e-10959.27unknown protein; CONTAINS InterPro DOMAIN/s: Protein of unknown function DUF602 ... [more]
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 249..280
NoneNo IPR availablePFAMPF04641Rtf2coord: 86..366
e-value: 9.1E-74
score: 248.1
NoneNo IPR availablePANTHERPTHR12775:SF1BNACNNG39770D PROTEINcoord: 7..297
NoneNo IPR availablePANTHERPTHR12775:SF1BNACNNG39770D PROTEINcoord: 328..382
IPR006735Replication termination factor 2PANTHERPTHR12775PROTEIN C20ORF43 HOMOLOGcoord: 328..382
IPR006735Replication termination factor 2PANTHERPTHR12775PROTEIN C20ORF43 HOMOLOGcoord: 7..297
IPR027799Replication termination factor 2, RING-fingerCDDcd16653RING-like_Rtf2coord: 196..241
e-value: 2.57939E-26
score: 97.6967

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0003287.1Tan0003287.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902979 mitotic DNA replication termination
cellular_component GO:0005634 nucleus