Tan0003265 (gene) Snake gourd v1

BLAST of Tan0003265 vs. ExPASy Swiss-Prot
Match: Q9SEY5 (Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1)

HSP 1 Score: 496.5 bits (1277), Expect = 3.7e-139
Identity = 266/560 (47.50%), Postives = 366/560 (65.36%), Query Frame = 0

Query: 1   MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
           +E    SPANF+PLSP+ FL+R++ VY  R S+V+G    TW +TY RCL+LASAL  + 
Sbjct: 48  IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALT-NL 107

Query: 61  HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
            +S GD+VAA+APN+P ++ELHFAVPMAG I+CPLNT+LD +TLS+LL   E KI+F+D 
Sbjct: 108 GISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDH 167

Query: 121 QYLPI---VLKSLSQNGKFNIKIPALVLIPADLDTPPSEFP-----------DYNRVLEM 180
           Q L I    L  L+++ K   K   LVLI    D   S+             DY     +
Sbjct: 168 QLLEIAHGALDLLAKSDKTR-KSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLL 227

Query: 181 RFGDF---TSKPNGELDPISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGICNTPSP 240
           + GD      KP  E DPISIN TSG+T   KG +YSHR AYLNSLAT+F   +  +  P
Sbjct: 228 KSGDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQM--SVYP 287

Query: 241 VFLWTVDMFRCNGWCFIWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMI 300
           V+LWTV MF CNGWC +W +AA GG NICLR V+   IF ++ +HKVT + G  T+L MI
Sbjct: 288 VYLWTVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMI 347

Query: 301 SE--SSHPKPLSRRVDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPN 360
                +  KPL  RV+++  G+ P+ +IL K+ E GFN+S+ YG+TE  GP     WKP 
Sbjct: 348 VNYTVTEHKPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPE 407

Query: 361 FDEETVQFEDLITSLE---------IDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYK 420
           +D  +++    + + +         +DVKDP++ME+V  DG T+GEVM RGNT+MSGY+K
Sbjct: 408 WDSLSLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFK 467

Query: 421 NLKATCEAFSGDWYRTGDVAIRHKSGRIEMKDRAKDIVVRPNGEAVSTVAVEAVLMSHSK 480
           +++AT +AF GDW+ +GD+A+++  G IE+KDR KD+++   GE +S+V VE VL SH  
Sbjct: 468 DIEATRKAFEGDWFHSGDLAVKYPDGYIEIKDRLKDVII-SGGENISSVEVERVLCSHQA 527

Query: 481 VAEAAVIGKFEVGFGERLCGFVKLKNG-SKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPM 532
           V EAAV+ + +  +G+  CGFVKLK G   +  EEII FCR HLP +M P+ +VFGD+P 
Sbjct: 528 VLEAAVVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPK 587

BLAST of Tan0003265 vs. ExPASy Swiss-Prot
Match: M4IS92 (Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1)

HSP 1 Score: 490.3 bits (1261), Expect = 2.7e-137
Identity = 264/546 (48.35%), Postives = 353/546 (64.65%), Query Frame = 0

Query: 1   MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
           ME      AN  PLSP+ FL+R+S  Y    S+VYG   +TW++T+ RCLKLASAL  HF
Sbjct: 29  MEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHF 88

Query: 61  HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
            +SPGD+VA  + NIPE+YELHFAVPMAG I+C LN + D+A +S LL   E K+IF++ 
Sbjct: 89  GISPGDVVATFSYNIPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEP 148

Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDTPPSEFPDYNRVLEMRFGDF-TSKPNGEL 180
           Q L     +L    + +IK P LVL+        S +  YN +L     DF   +P  E 
Sbjct: 149 QLLETARAALDLLAQKDIKPPTLVLLTDSESFTSSSYDHYNHLLANGSDDFEIRRPKNEC 208

Query: 181 DPISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGICNTPSPVFLWTVDMFRCNGWCF 240
           DPISIN TSG+T   K  +YSHR AYLNS+AT+   G+  T S V+LW+V MF CNGWCF
Sbjct: 209 DPISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGM-GTRS-VYLWSVPMFHCNGWCF 268

Query: 241 IWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMI---SESSHPKPLSRRV 300
            W  AA G  NIC+R V+  AIF+++ LHKVT      T+L MI    E +   PL  +V
Sbjct: 269 PWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLHTPLPHKV 328

Query: 301 DLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFD----EETVQFE-- 360
           +++  G+ P  +++ ++ E GF +++ YG+TE  GPA     KP +D    EE    +  
Sbjct: 329 EVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETHGPATNCVCKPEWDALQPEERYALKAR 388

Query: 361 ---DLITSLEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYR 420
              + +   E+DV+DP+SMESV ADG T+GEVM RGNT+MSGY+K+LKAT EAF G W+R
Sbjct: 389 QGLNHLAMEEMDVRDPVSMESVRADGTTIGEVMFRGNTVMSGYFKDLKATEEAFEGGWFR 448

Query: 421 TGDVAIRHKSGRIEMKDRAKDIVVRPNGEAVSTVAVEAVLMSHSKVAEAAVIGKFEVGFG 480
           TGD+ ++H+ G I++KDR KD+V+   GE VSTV VE VL SH  V EAAV+ + +  +G
Sbjct: 449 TGDLGVKHEDGYIQLKDRKKDVVI-SGGENVSTVEVETVLYSHEAVLEAAVVARPDKLWG 508

Query: 481 ERLCGFVKLKNG--SKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAVREK 532
           E  C FV LK G  + VS ++IIKFCR  LP +M P+ VVF +LP  STGKIQK+ ++EK
Sbjct: 509 ETPCAFVTLKEGFDNGVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQKYILKEK 568

BLAST of Tan0003265 vs. ExPASy Swiss-Prot
Match: M4IRL4 (Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1)

HSP 1 Score: 484.2 bits (1245), Expect = 1.9e-135
Identity = 257/546 (47.07%), Postives = 350/546 (64.10%), Query Frame = 0

Query: 1   MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
           ME      AN  PLSP+ FL+R+S  Y    S+VYG   +TW++T+ RCLKLASAL  H 
Sbjct: 29  MEGLVQCSANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHL 88

Query: 61  HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
            +SPGD+VA  + N+PE+YELHFAVPMAG I+C LN + D+A +S LL   E K+IF++ 
Sbjct: 89  GISPGDVVATFSYNLPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEP 148

Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDTPPSEFPDYNRVLEMRFGDF-TSKPNGEL 180
           Q L     +L    + +IK P LVL+        S +  YN +L     DF   +P  E 
Sbjct: 149 QLLETARAALDLLAQKDIKPPTLVLLTDSESFTSSSYDHYNHLLANGSDDFEIRRPKNEW 208

Query: 181 DPISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGICNTPSPVFLWTVDMFRCNGWCF 240
           DPISIN TSG+T   K  +YSHR AYLNS+AT+   G+  T   V+LW+V MF CNGWCF
Sbjct: 209 DPISINYTSGTTARPKAVVYSHRGAYLNSIATVLLHGMGTT--SVYLWSVPMFHCNGWCF 268

Query: 241 IWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMI---SESSHPKPLSRRV 300
            W  AA G  NIC+R V+  AIF+++ LHKVT      T+L MI    E +   PL  +V
Sbjct: 269 PWGAAAQGATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLHTPLPHKV 328

Query: 301 DLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFD----EETVQFE-- 360
           +++  G+ P  +++ ++ E GF +++ YG+TE  GPA     KP +D    EE    +  
Sbjct: 329 EVMTGGSPPPPKVIARMEEMGFQVNHIYGLTETCGPAANCVCKPEWDALQPEERYALKAR 388

Query: 361 ---DLITSLEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYR 420
              + +   E+DV+DP++MESV ADG T+GEVM RGNT+MSGY+K+LKAT EAF G W+R
Sbjct: 389 QGLNHLAMEEMDVRDPVTMESVRADGATIGEVMFRGNTVMSGYFKDLKATEEAFEGGWFR 448

Query: 421 TGDVAIRHKSGRIEMKDRAKDIVVRPNGEAVSTVAVEAVLMSHSKVAEAAVIGKFEVGFG 480
           +GD+ ++H+ G I++KDR KD+V+   GE +STV VE VL SH  V EAAV+ + +  +G
Sbjct: 449 SGDLGVKHEDGYIQLKDRKKDVVI-SGGENISTVEVETVLYSHEAVLEAAVVARPDKLWG 508

Query: 481 ERLCGFVKLKNG--SKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAVREK 532
           E  C FV LK G  + VS ++IIKFCR  LP +M P+ VVF +LP  STGKIQK+ ++EK
Sbjct: 509 ETPCAFVTLKEGFDNDVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQKYILKEK 568

BLAST of Tan0003265 vs. ExPASy Swiss-Prot
Match: F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)

HSP 1 Score: 479.2 bits (1232), Expect = 6.2e-134
Identity = 250/547 (45.70%), Postives = 347/547 (63.44%), Query Frame = 0

Query: 1   MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
           ME    SPAN+ PL+P+ FL R++ VY  R S+VYG   +TW +T  RC+++ASAL    
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASAL-SQL 62

Query: 61  HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
            +S GD+V+ +APN+P + ELHF VPMAGA++C LN + D++ +++LL+    K+IF D 
Sbjct: 63  GISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADH 122

Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDTPPS------EFPDYNRVLEMRFGDF-TS 180
           Q+L I   +         K+P LVLIP  L    S      E  +Y  V+ M   DF   
Sbjct: 123 QFLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVI 182

Query: 181 KPNGELDPISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGICNTPSPVFLWTVDMFR 240
           +P  E D IS+N TSG+T   KG +YSHR AYLNSLA +  + + +  SP +LWT  MF 
Sbjct: 183 RPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHS--SPTYLWTNPMFH 242

Query: 241 CNGWCFIWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMI--SESSHPKP 300
           CNGWC +W + A+GG NICLR VT  AIF+++  HKVT + G  T+L MI  +  S  KP
Sbjct: 243 CNGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKP 302

Query: 301 LSRRVDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFD----EETV 360
           L  +V  I   A P   ++ K+ E GF++ + YG+TE  GP  I  WKP +D    EE  
Sbjct: 303 LPGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQA 362

Query: 361 QFE-----DLITSLEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFS 420
           + +     + +   EI VKDP++M ++ ADG T+GEV+ RGNT+M+GY KN +AT EAF 
Sbjct: 363 KMKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFK 422

Query: 421 GDWYRTGDVAIRHKSGRIEMKDRAKDIVVRPNGEAVSTVAVEAVLMSHSKVAEAAVIGKF 480
           G W+ +GD+ ++H  G IE+KDR+KDI++   GE +S++ VE+ L +H  V EAAV+ + 
Sbjct: 423 GGWFWSGDLGVKHPDGYIELKDRSKDIII-SGGENISSIEVESTLFTHPCVLEAAVVARP 482

Query: 481 EVGFGERLCGFVKLKNGSKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAV 530
           +  +GE  C FVKLK+GSK S EE+I +CR  LP +M P  +VF DLP  STGK+QKF +
Sbjct: 483 DEYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVL 542

BLAST of Tan0003265 vs. ExPASy Swiss-Prot
Match: Q9C8D4 (Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11 PE=1 SV=1)

HSP 1 Score: 409.5 bits (1051), Expect = 6.0e-113
Identity = 222/556 (39.93%), Postives = 348/556 (62.59%), Query Frame = 0

Query: 1   MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
           M++     AN  PL+P+ FLKRAS  Y  R S++YG   FTW +TY RC +LA++L+   
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLL-SL 60

Query: 61  HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
           +++  D+V+ +APN+P +YE+HF+VPM GA++ P+NT+LDA T++++L+  EPKI+F+D 
Sbjct: 61  NITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDY 120

Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDT---PPSEFPDYNRVLEMRFGDFTS---- 180
           ++ P++ + L     +  +    +++  ++D+   P S+  DY  ++  R G+ T     
Sbjct: 121 EFAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLI--RKGEPTPSSSA 180

Query: 181 ---KPNGELDPISINCTSGSTGLHKGAIYSHRAAYLNSLATI--FRSGICNTPSPVFLWT 240
              + + E DPIS+N TSG+T   KG + SH+ AYL++L++I  +  GI     PV+LWT
Sbjct: 181 SMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGI----FPVYLWT 240

Query: 241 VDMFRCNGWCFIWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMISESSH 300
           + MF CNGW   W++AA GG N+C+R VT   I+ ++ELH VT +    T+ + + E S 
Sbjct: 241 LPMFHCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSR 300

Query: 301 --PKPLSRRVDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDE-- 360
               P S  V ++  G+ P   ++ KV + GF++ +GYG+TEA GP +   W+  +++  
Sbjct: 301 TDQSPKSSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLP 360

Query: 361 ETVQFE-------DLITSLEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKAT 420
           E  Q E         +T  ++DVK+  ++ESV  DG+T+GE++++G++LM GY KN KAT
Sbjct: 361 EHQQIELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKAT 420

Query: 421 CEAFSGDWYRTGDVAIRHKSGRIEMKDRAKDIVVRPNGEAVSTVAVEAVLMSHSKVAEAA 480
            EAF   W  TGD+ + H  G +E+KDR+KDI++   GE +S++ VE VL  + +V EAA
Sbjct: 421 SEAFKHGWLNTGDIGVIHPDGYVEIKDRSKDIII-SGGENISSIEVEKVLYMYQEVLEAA 480

Query: 481 VIGKFEVGFGERLCGFVKLKNGSK---VSVEEIIKFCRAHLPEFMVPEKVV-FGDLPMNS 530
           V+      +GE  C FV LK G +    S  ++IK+CR ++P FM P+KVV F +LP NS
Sbjct: 481 VVAMPHPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNS 540

BLAST of Tan0003265 vs. NCBI nr
Match: XP_023007650.1 (probable acyl-activating enzyme 1, peroxisomal [Cucurbita maxima])

HSP 1 Score: 874.8 bits (2259), Expect = 3.9e-250
Identity = 440/540 (81.48%), Postives = 474/540 (87.78%), Query Frame = 0

Query: 1   MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
           M+ FF SPANF PLSP+ F KRA+AVYGGRPSV+YG ++FTWSETYGRCL LASALVHHF
Sbjct: 42  MDPFFPSPANFVPLSPLAFFKRAAAVYGGRPSVIYGSRVFTWSETYGRCLHLASALVHHF 101

Query: 61  HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
           H+SPGD+VAAMAPN+PELYELHFAVPMAGAII PLNTKLDA TLSLLLQQL PK+IF+DS
Sbjct: 102 HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDS 161

Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDTPPSEFPDYNRVLEMRFGDFTSKPNGELD 180
            +LP +L+SLSQ    +I+ PALVLIPA  DTPPSEF DYNRVLEMRF +FT + N ELD
Sbjct: 162 DFLPTLLQSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLEMRFDEFTPRTNSELD 221

Query: 181 PISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGIC-NTPSPVFLWTVDMFRCNGWCF 240
            ISINCTSGSTGLHKG +YSHRAAYLNSLATIFRSGIC NT SPVFLWTVDMFRCNGWCF
Sbjct: 222 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 281

Query: 241 IWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMISESS---HPKP-LSRR 300
           IWAMAALGGCNICLR VT DAIF ++ELHKVTLLCGP+TLLKMI ESS   + KP   RR
Sbjct: 282 IWAMAALGGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNCKPGFLRR 341

Query: 301 VDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEETVQFEDLITS 360
           VDLIVAGALPI EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNFDEETVQFEDLI S
Sbjct: 342 VDLIVAGALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLIAS 401

Query: 361 LEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
           LEIDVKDP SMESVL DGETLGEVMLRGN+LMSGYYKNLKAT EAFSGDWYRTGDV ++H
Sbjct: 402 LEIDVKDPSSMESVLGDGETLGEVMLRGNSLMSGYYKNLKATGEAFSGDWYRTGDVGVKH 461

Query: 421 KSGRIEMKDRAKDIVVRPNGE-AVSTVAVEAVLMSHSKVAEAAVIGKFEVGFGERLCGFV 480
           KSGRIEMKDRAKDIVVR NGE AVSTV VEAVLMSH  VAEAAVI       GERLCG V
Sbjct: 462 KSGRIEMKDRAKDIVVRENGEGAVSTVQVEAVLMSHPGVAEAAVI-------GERLCGLV 521

Query: 481 KLKNGSKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAVREKAKALNAYNT 535
           KLKNGS+ S EEI++FCRAHLPEFM+PE+VVFGDLPMNS GK+QKF  REKAKALN  NT
Sbjct: 522 KLKNGSRASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTNNT 574

BLAST of Tan0003265 vs. NCBI nr
Match: XP_022923197.1 (probable acyl-activating enzyme 1, peroxisomal [Cucurbita moschata])

HSP 1 Score: 874.4 bits (2258), Expect = 5.0e-250
Identity = 442/540 (81.85%), Postives = 475/540 (87.96%), Query Frame = 0

Query: 1   MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
           M+ F  SPANF PLSPV FLK+++AVY  RPSV+YG ++FTWSETYGR L LASALVHHF
Sbjct: 49  MDPFSPSPANFVPLSPVTFLKKSAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 108

Query: 61  HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
           HVSPGD+VAAMAPN+PELYELHFAVPMAGAII PLNTKLDA TLSLLLQQL PK+IF+DS
Sbjct: 109 HVSPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 168

Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDTPPSEFPDYNRVLEMRFGDFTSKPNGELD 180
            +LP VL+SLSQ    +I+ PALVLIPA  DTPPSEF DYNRVL MRF DFT + N ELD
Sbjct: 169 DFLPTVLQSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD 228

Query: 181 PISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGIC-NTPSPVFLWTVDMFRCNGWCF 240
            ISINCTSGSTGLHKG +YSHRAAYLNSLATIFRSGIC NT SPVFLWTVDMFRCNGWCF
Sbjct: 229 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 288

Query: 241 IWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMISESS---HPKP-LSRR 300
           IWAMAALGGCNICLR VT DAIF ++ELHKVTLLCGP+TLLKMI ESS   + KP LSRR
Sbjct: 289 IWAMAALGGCNICLRNVTTDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWLSRR 348

Query: 301 VDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEETVQFEDLITS 360
           VDLIVAGALPI+EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNFDEETVQFEDLITS
Sbjct: 349 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLITS 408

Query: 361 LEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
           LEIDVKDP+SMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAFSGDWYRTGDV +RH
Sbjct: 409 LEIDVKDPLSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 468

Query: 421 KSGRIEMKDRAKDIVVRPNGE-AVSTVAVEAVLMSHSKVAEAAVIGKFEVGFGERLCGFV 480
           KSGRIEMKDRAKDIVVR NGE AVSTV VEAVLMSH  VAEAAV+       GERLCGFV
Sbjct: 469 KSGRIEMKDRAKDIVVRANGEVAVSTVQVEAVLMSHPSVAEAAVV-------GERLCGFV 528

Query: 481 KLKNGSKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAVREKAKALNAYNT 535
           KLKNGS+ S EEI+KFCR HLPEFM+PE+VVFGDLPMNS GK+QKF  REKAKALN  +T
Sbjct: 529 KLKNGSRASAEEIVKFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTNST 581

BLAST of Tan0003265 vs. NCBI nr
Match: KAG7015844.1 (putative acyl-activating enzyme 1, peroxisomal, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 872.1 bits (2252), Expect = 2.5e-249
Identity = 441/540 (81.67%), Postives = 472/540 (87.41%), Query Frame = 0

Query: 1   MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
           M+ F  SPANF PLSPV F KRA+AVY  RPSV+YG ++FTWSETYGR L LASALVHHF
Sbjct: 1   MDPFSPSPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 60

Query: 61  HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
           H+SPGD+VAAMAPN+PELYELHFAVPMAGAII PLNTKLDA TLSLLLQQL PK+IF+DS
Sbjct: 61  HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 120

Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDTPPSEFPDYNRVLEMRFGDFTSKPNGELD 180
            +LP VLKSLSQ    +I+ PALVLIPA  DTPPSEF DYNRVL MRF DFT + N ELD
Sbjct: 121 DFLPTVLKSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD 180

Query: 181 PISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGIC-NTPSPVFLWTVDMFRCNGWCF 240
            ISINCTSGSTGLHKG +YSHRAAYLNSLATIFRSGIC NT SPVFLWTVDMFRCNGWCF
Sbjct: 181 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 240

Query: 241 IWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMISESS---HPKP-LSRR 300
           IWAMAALGGCNICLR VT DAIF ++ELHKVTLLCGP+TLLKMI ESS   + KP  SRR
Sbjct: 241 IWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRR 300

Query: 301 VDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEETVQFEDLITS 360
           VDLIVAGALPI+EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNFDEETVQFEDL TS
Sbjct: 301 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLFTS 360

Query: 361 LEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
           LEIDVKDPMSMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAFSGDWYRTGDV +RH
Sbjct: 361 LEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 420

Query: 421 KSGRIEMKDRAKDIVVRPNGEA-VSTVAVEAVLMSHSKVAEAAVIGKFEVGFGERLCGFV 480
           KSGRIEMKDRAKDIVVR NGEA VSTV VEAVLMSH  VAEAAV+       GERLCGFV
Sbjct: 421 KSGRIEMKDRAKDIVVRANGEATVSTVQVEAVLMSHPSVAEAAVV-------GERLCGFV 480

Query: 481 KLKNGSKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAVREKAKALNAYNT 535
           KLKNGS+ S EEI++FCR HLPEFM+PE+VVFGDLPMNS GK+QKF VREKAK LN  +T
Sbjct: 481 KLKNGSRASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKELNTNST 533

BLAST of Tan0003265 vs. NCBI nr
Match: KAG6577804.1 (putative acyl-activating enzyme 1, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 868.2 bits (2242), Expect = 3.6e-248
Identity = 439/540 (81.30%), Postives = 470/540 (87.04%), Query Frame = 0

Query: 1   MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
           M+ F  SPANF PLSPV F KRA+AVY  RPSV+YG ++FTWSETYGR L LASALVHHF
Sbjct: 1   MDPFSPSPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 60

Query: 61  HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
           H+SPGD+VAAMAPN+PELYELHFAVPMAGAII PLNTKLDA TLSLLLQQL PK+IF+DS
Sbjct: 61  HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 120

Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDTPPSEFPDYNRVLEMRFGDFTSKPNGELD 180
            +LP VLKSLSQ    +I+ PALVLIP   DTPPSEF DYNRVL MRF DFT + N ELD
Sbjct: 121 DFLPTVLKSLSQTYGNSIQFPALVLIPTHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD 180

Query: 181 PISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGIC-NTPSPVFLWTVDMFRCNGWCF 240
            ISINCTSGSTGLHKG +YSHRAAYLNSLATIFRSGIC NT SPVFLWTVDMFRCNGWCF
Sbjct: 181 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 240

Query: 241 IWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMISESS---HPKP-LSRR 300
           IWAMAALGGCNICLR VT DAIF ++ELHKVTLLCGP+TLLKMI ESS   + KP  SRR
Sbjct: 241 IWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRR 300

Query: 301 VDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEETVQFEDLITS 360
           VDLIVAGALPI+EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNFDEE VQFEDL TS
Sbjct: 301 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEEIVQFEDLFTS 360

Query: 361 LEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
           LEIDVKDPMSMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAFSGDWYRTGDV +RH
Sbjct: 361 LEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 420

Query: 421 KSGRIEMKDRAKDIVVRPNGE-AVSTVAVEAVLMSHSKVAEAAVIGKFEVGFGERLCGFV 480
           KSGRIEMKDRAKDIVVR NGE AVSTV VEAVLMSH  VAEAAV+       GERLCGFV
Sbjct: 421 KSGRIEMKDRAKDIVVRANGEAAVSTVQVEAVLMSHPSVAEAAVV-------GERLCGFV 480

Query: 481 KLKNGSKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAVREKAKALNAYNT 535
           KLKNGS+ S EEI++FCR HLPEFM+PE+VVFGDLPMNS GK+QKF VREKAK LN  +T
Sbjct: 481 KLKNGSRASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKELNTNST 533

BLAST of Tan0003265 vs. NCBI nr
Match: XP_023552791.1 (probable acyl-activating enzyme 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 866.3 bits (2237), Expect = 1.4e-247
Identity = 436/540 (80.74%), Postives = 473/540 (87.59%), Query Frame = 0

Query: 1   MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
           M+ F   PANF PLSP+ F  +++AVYG RPS++YG ++FTWSETYGRCL LASALVHHF
Sbjct: 1   MDPFSPFPANFVPLSPLTFFNKSAAVYGARPSLIYGARVFTWSETYGRCLHLASALVHHF 60

Query: 61  HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
           H+SPGD+VAAMAPN+PELYELHFAVPMAGAII PLNTKLDA TLSLLLQQL+PK+IF+DS
Sbjct: 61  HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLDPKLIFLDS 120

Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDTPPSEFPDYNRVLEMRFGDFTSKPNGELD 180
            +LP VLKSLSQ    +++ PALVLIPA  DTP SEF DYNRVL MRF DFT + N ELD
Sbjct: 121 DFLPTVLKSLSQTYGNSVQFPALVLIPAHPDTPLSEFLDYNRVLGMRFDDFTPRTNAELD 180

Query: 181 PISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGIC-NTPSPVFLWTVDMFRCNGWCF 240
            ISINCTSGSTGLHKG +YSHRAAYLNSLATIFRSGIC NT SPVFLWTVDMFRCNGWCF
Sbjct: 181 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 240

Query: 241 IWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMISESS---HPKP-LSRR 300
           IWAMAALGGCNICLR VT + IF ++ELHKVTLLCGP+TLLKMI ESS   + KP LSRR
Sbjct: 241 IWAMAALGGCNICLRNVTTNVIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWLSRR 300

Query: 301 VDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEETVQFEDLITS 360
           VDLIVAGALPI+EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNF EETVQFEDLITS
Sbjct: 301 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFGEETVQFEDLITS 360

Query: 361 LEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
           LEIDVKDP+SMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAFSGDWYRTGDV +RH
Sbjct: 361 LEIDVKDPLSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 420

Query: 421 KSGRIEMKDRAKDIVVRPNGE-AVSTVAVEAVLMSHSKVAEAAVIGKFEVGFGERLCGFV 480
           KSGRIEMKDRAKDIVVR NGE AVSTV VEAVLMSH  V EAAVI       GERLCGFV
Sbjct: 421 KSGRIEMKDRAKDIVVRANGEAAVSTVEVEAVLMSHPSVVEAAVI-------GERLCGFV 480

Query: 481 KLKNGSKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAVREKAKALNAYNT 535
           KLKNGS+ S EEI++FCRAHLPEFM+PE+VVFGDLPMNS GK+QKF VREKAKALN  NT
Sbjct: 481 KLKNGSRASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKALNTNNT 533

BLAST of Tan0003265 vs. ExPASy TrEMBL
Match: A0A6J1L3K3 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111500216 PE=4 SV=1)

HSP 1 Score: 874.8 bits (2259), Expect = 1.9e-250
Identity = 440/540 (81.48%), Postives = 474/540 (87.78%), Query Frame = 0

Query: 1   MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
           M+ FF SPANF PLSP+ F KRA+AVYGGRPSV+YG ++FTWSETYGRCL LASALVHHF
Sbjct: 42  MDPFFPSPANFVPLSPLAFFKRAAAVYGGRPSVIYGSRVFTWSETYGRCLHLASALVHHF 101

Query: 61  HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
           H+SPGD+VAAMAPN+PELYELHFAVPMAGAII PLNTKLDA TLSLLLQQL PK+IF+DS
Sbjct: 102 HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDS 161

Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDTPPSEFPDYNRVLEMRFGDFTSKPNGELD 180
            +LP +L+SLSQ    +I+ PALVLIPA  DTPPSEF DYNRVLEMRF +FT + N ELD
Sbjct: 162 DFLPTLLQSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLEMRFDEFTPRTNSELD 221

Query: 181 PISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGIC-NTPSPVFLWTVDMFRCNGWCF 240
            ISINCTSGSTGLHKG +YSHRAAYLNSLATIFRSGIC NT SPVFLWTVDMFRCNGWCF
Sbjct: 222 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 281

Query: 241 IWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMISESS---HPKP-LSRR 300
           IWAMAALGGCNICLR VT DAIF ++ELHKVTLLCGP+TLLKMI ESS   + KP   RR
Sbjct: 282 IWAMAALGGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNCKPGFLRR 341

Query: 301 VDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEETVQFEDLITS 360
           VDLIVAGALPI EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNFDEETVQFEDLI S
Sbjct: 342 VDLIVAGALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLIAS 401

Query: 361 LEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
           LEIDVKDP SMESVL DGETLGEVMLRGN+LMSGYYKNLKAT EAFSGDWYRTGDV ++H
Sbjct: 402 LEIDVKDPSSMESVLGDGETLGEVMLRGNSLMSGYYKNLKATGEAFSGDWYRTGDVGVKH 461

Query: 421 KSGRIEMKDRAKDIVVRPNGE-AVSTVAVEAVLMSHSKVAEAAVIGKFEVGFGERLCGFV 480
           KSGRIEMKDRAKDIVVR NGE AVSTV VEAVLMSH  VAEAAVI       GERLCG V
Sbjct: 462 KSGRIEMKDRAKDIVVRENGEGAVSTVQVEAVLMSHPGVAEAAVI-------GERLCGLV 521

Query: 481 KLKNGSKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAVREKAKALNAYNT 535
           KLKNGS+ S EEI++FCRAHLPEFM+PE+VVFGDLPMNS GK+QKF  REKAKALN  NT
Sbjct: 522 KLKNGSRASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTNNT 574

BLAST of Tan0003265 vs. ExPASy TrEMBL
Match: A0A6J1E5I3 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111430963 PE=4 SV=1)

HSP 1 Score: 874.4 bits (2258), Expect = 2.4e-250
Identity = 442/540 (81.85%), Postives = 475/540 (87.96%), Query Frame = 0

Query: 1   MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
           M+ F  SPANF PLSPV FLK+++AVY  RPSV+YG ++FTWSETYGR L LASALVHHF
Sbjct: 49  MDPFSPSPANFVPLSPVTFLKKSAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 108

Query: 61  HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
           HVSPGD+VAAMAPN+PELYELHFAVPMAGAII PLNTKLDA TLSLLLQQL PK+IF+DS
Sbjct: 109 HVSPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 168

Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDTPPSEFPDYNRVLEMRFGDFTSKPNGELD 180
            +LP VL+SLSQ    +I+ PALVLIPA  DTPPSEF DYNRVL MRF DFT + N ELD
Sbjct: 169 DFLPTVLQSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD 228

Query: 181 PISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGIC-NTPSPVFLWTVDMFRCNGWCF 240
            ISINCTSGSTGLHKG +YSHRAAYLNSLATIFRSGIC NT SPVFLWTVDMFRCNGWCF
Sbjct: 229 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 288

Query: 241 IWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMISESS---HPKP-LSRR 300
           IWAMAALGGCNICLR VT DAIF ++ELHKVTLLCGP+TLLKMI ESS   + KP LSRR
Sbjct: 289 IWAMAALGGCNICLRNVTTDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWLSRR 348

Query: 301 VDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEETVQFEDLITS 360
           VDLIVAGALPI+EILTKVTE GFNISYGYGMTEA+GPA+IRPWKPNFDEETVQFEDLITS
Sbjct: 349 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLITS 408

Query: 361 LEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRH 420
           LEIDVKDP+SMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAFSGDWYRTGDV +RH
Sbjct: 409 LEIDVKDPLSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 468

Query: 421 KSGRIEMKDRAKDIVVRPNGE-AVSTVAVEAVLMSHSKVAEAAVIGKFEVGFGERLCGFV 480
           KSGRIEMKDRAKDIVVR NGE AVSTV VEAVLMSH  VAEAAV+       GERLCGFV
Sbjct: 469 KSGRIEMKDRAKDIVVRANGEVAVSTVQVEAVLMSHPSVAEAAVV-------GERLCGFV 528

Query: 481 KLKNGSKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAVREKAKALNAYNT 535
           KLKNGS+ S EEI+KFCR HLPEFM+PE+VVFGDLPMNS GK+QKF  REKAKALN  +T
Sbjct: 529 KLKNGSRASAEEIVKFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTNST 581

BLAST of Tan0003265 vs. ExPASy TrEMBL
Match: A0A1S3BKP8 (probable acyl-activating enzyme 2 OS=Cucumis melo OX=3656 GN=LOC103490658 PE=4 SV=1)

HSP 1 Score: 804.7 bits (2077), Expect = 2.4e-229
Identity = 415/541 (76.71%), Postives = 461/541 (85.21%), Query Frame = 0

Query: 1   MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
           ME F H P NFAPLSPV FLKRAS +YG RPS++YG ++FTWS+TYGRCL +ASALVHHF
Sbjct: 1   MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF 60

Query: 61  HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
           H+SP DLV AMAPNIPELYELHFAVPMAG II  LNTKLD+ TLSLLLQQL PK+IF+DS
Sbjct: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120

Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDTP-PSEFPDYNRVLEMRFG--DFTSKPNG 180
           Q+LPI+LKSL      +IK PAL+LIP+D +T  PS+F DYN++L MR G  +FT +PN 
Sbjct: 121 QFLPILLKSLE---NISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNA 180

Query: 181 ELDPISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGIC-NTPSPVFLWTVDMFRCNG 240
           ELDPISIN TSGSTGLHKG IYSHRAAYLNSLATIFRS IC +T SPVFLWTVDMFRCNG
Sbjct: 181 ELDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNG 240

Query: 241 WCFIWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMISESSH----PKPL 300
           WCFIW MAALGGCNICLRTVT DAIF +VELH+VTLLCGP+TLLKMI ESS     P+ L
Sbjct: 241 WCFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRL 300

Query: 301 SRRVDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNF-DEETVQFED 360
           SRRVDLIVAGALPI EILTKV E GFNISYGYGMTEA+GPAIIRPWKP+F D++ VQF+D
Sbjct: 301 SRRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDD 360

Query: 361 -LITSLEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGD-WYRTG 420
            LITSLEIDVKDP+SMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAF G+ WYRTG
Sbjct: 361 HLITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTG 420

Query: 421 DVAIRHKSGRIEMKDRAKDIVVRPNGE-AVSTVAVEAVLMSHSKVAEAAVIGKFEVGFGE 480
           DV +RHKSGRIEMKDRAKDIVVR +GE AVSTV VE VLMSH  VAE AV+G+       
Sbjct: 421 DVGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGE------R 480

Query: 481 RLCGFVKLKNGSKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAVREKAKA 530
            L GFVKLKNGSK + +EI++FCR HLPEFMVP+ +VFGDLPMNSTGK+QKF +REKAKA
Sbjct: 481 TLYGFVKLKNGSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKA 532

BLAST of Tan0003265 vs. ExPASy TrEMBL
Match: A0A0A0L3L0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G002400 PE=4 SV=1)

HSP 1 Score: 714.9 bits (1844), Expect = 2.5e-202
Identity = 375/475 (78.95%), Postives = 408/475 (85.89%), Query Frame = 0

Query: 71  MAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDSQYLPIVLKSL 130
           MAPNIPELYELHFAVPMAGAII  LNTKLD+ TLSLLLQQL PK+IFIDSQ+LPI+LKSL
Sbjct: 1   MAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFIDSQFLPILLKSL 60

Query: 131 SQNGKFNIKIPALVLIPADLDTP-PSEFPDYNRVLEMRFG--DFTSKPNGELDPISINCT 190
             +   +IK PALVLIP+D DTP PSEF DYN+VL MRFG  DFT +PN ELDPISIN T
Sbjct: 61  ENS---SIKFPALVLIPSDPDTPLPSEFLDYNKVLAMRFGDDDFTPRPNAELDPISINYT 120

Query: 191 SGSTGLHKGAIYSHRAAYLNSLATIFRSGIC-NTPSPVFLWTVDMFRCNGWCFIWAMAAL 250
           SGSTGLHKG IYSHRAAYLNSLATIFRS IC +T SPVFLWTVDMFRCNGWCFIW MAAL
Sbjct: 121 SGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAAL 180

Query: 251 GGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMISESSH----PKPLSRRVDLIVAG 310
           GGCNICLRTVT DAIF +VELH+VTLLCGP TLLKMI ESS     P+ LSRRVDLIVAG
Sbjct: 181 GGCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMIYESSSNNCMPRRLSRRVDLIVAG 240

Query: 311 ALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDEETVQFEDLITSLEIDVKD 370
           ALPI EILTKV E GFNISYGYGMTEA+GPAIIRPWKP F+E+ VQFEDLITSLEIDVKD
Sbjct: 241 ALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTFEEDNVQFEDLITSLEIDVKD 300

Query: 371 PMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGD-WYRTGDVAIRHKSGRIE 430
           P+SMESVL DGETLGEVMLRGNTLMSGYYKN+KAT EAF G+ WYRTGDV +RHKSGRIE
Sbjct: 301 PISMESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEAFIGENWYRTGDVGVRHKSGRIE 360

Query: 431 MKDRAKDIVVRPNGE-AVSTVAVEAVLMSHSKVAEAAVIGKFEVGFGERLCGFVKLKNGS 490
           MKDRAKDIVVR +GE AVSTV VE VLMSH  VAEAAV+G+        L GFVKLKN S
Sbjct: 361 MKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAEAAVVGE------RTLYGFVKLKNRS 420

Query: 491 KVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAVREKAKAL--NAYN 534
           K + +EI++FCR HLPEFM+P+++VFGDLPMNSTGK+QKFA+REK KAL  NA N
Sbjct: 421 KENGDEIVEFCRMHLPEFMIPKRIVFGDLPMNSTGKVQKFALREKVKALLNNANN 466

BLAST of Tan0003265 vs. ExPASy TrEMBL
Match: A0A5A7UEI8 (Putative acyl-activating enzyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00360 PE=4 SV=1)

HSP 1 Score: 693.3 bits (1788), Expect = 7.7e-196
Identity = 364/471 (77.28%), Postives = 402/471 (85.35%), Query Frame = 0

Query: 71  MAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDSQYLPIVLKSL 130
           MAPNIPELYELHFAVPMAG II  LNTKLD+ TLSLLLQQL PK+IF+DSQ+LPI+LKSL
Sbjct: 1   MAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDSQFLPILLKSL 60

Query: 131 SQNGKFNIKIPALVLIPADLDTP-PSEFPDYNRVLEMRFG--DFTSKPNGELDPISINCT 190
                 +IK PAL+LIP+D +T  PS+F DYN++L MR G  +FT +PN ELDPISIN T
Sbjct: 61  E---NISIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELDPISINYT 120

Query: 191 SGSTGLHKGAIYSHRAAYLNSLATIFRSGIC-NTPSPVFLWTVDMFRCNGWCFIWAMAAL 250
           SGSTGLHKG IYSHRAAYLNSLATIFRS IC +T SPVFLWTVDMFRCNGWCFIW MAAL
Sbjct: 121 SGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAAL 180

Query: 251 GGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMISESSH----PKPLSRRVDLIVAG 310
           GGCNICLRTVT DAIF +VELH+VTLLCGP+TLLKMI ESS     P+ LSRRVDLIVAG
Sbjct: 181 GGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRRVDLIVAG 240

Query: 311 ALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNF-DEETVQFED-LITSLEIDV 370
           ALPI EILTKV E GFNISYGYGMTEA+GPAIIRPWKP+F D++ VQF+D LITSLEIDV
Sbjct: 241 ALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLITSLEIDV 300

Query: 371 KDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGD-WYRTGDVAIRHKSGR 430
           KDP+SMESVL DGETLGEVMLRGNTLMSGYYKNLKAT EAF G+ WYRTGDV +RHKSGR
Sbjct: 301 KDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVGVRHKSGR 360

Query: 431 IEMKDRAKDIVVRPNGE-AVSTVAVEAVLMSHSKVAEAAVIGKFEVGFGERLCGFVKLKN 490
           IEMKDRAKDIVVR +GE AVSTV VE VLMSH  VAE AV+G+        L GFVKLKN
Sbjct: 361 IEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGE------RTLYGFVKLKN 420

Query: 491 GSKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAVREKAKAL 530
           GSK + +EI++FCR HLPEFMVP+ +VFGDLPMNSTGK+QKF +REKAKAL
Sbjct: 421 GSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKAL 462

BLAST of Tan0003265 vs. TAIR 10
Match: AT2G17650.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 496.5 bits (1277), Expect = 2.7e-140
Identity = 266/560 (47.50%), Postives = 366/560 (65.36%), Query Frame = 0

Query: 1   MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
           +E    SPANF+PLSP+ FL+R++ VY  R S+V+G    TW +TY RCL+LASAL  + 
Sbjct: 48  IEGLLRSPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALT-NL 107

Query: 61  HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
            +S GD+VAA+APN+P ++ELHFAVPMAG I+CPLNT+LD +TLS+LL   E KI+F+D 
Sbjct: 108 GISRGDVVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDH 167

Query: 121 QYLPI---VLKSLSQNGKFNIKIPALVLIPADLDTPPSEFP-----------DYNRVLEM 180
           Q L I    L  L+++ K   K   LVLI    D   S+             DY     +
Sbjct: 168 QLLEIAHGALDLLAKSDKTR-KSLKLVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLL 227

Query: 181 RFGDF---TSKPNGELDPISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGICNTPSP 240
           + GD      KP  E DPISIN TSG+T   KG +YSHR AYLNSLAT+F   +  +  P
Sbjct: 228 KSGDSEFEIIKPRCEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVFLHQM--SVYP 287

Query: 241 VFLWTVDMFRCNGWCFIWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMI 300
           V+LWTV MF CNGWC +W +AA GG NICLR V+   IF ++ +HKVT + G  T+L MI
Sbjct: 288 VYLWTVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMI 347

Query: 301 SE--SSHPKPLSRRVDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPN 360
                +  KPL  RV+++  G+ P+ +IL K+ E GFN+S+ YG+TE  GP     WKP 
Sbjct: 348 VNYTVTEHKPLPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKPE 407

Query: 361 FDEETVQFEDLITSLE---------IDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYK 420
           +D  +++    + + +         +DVKDP++ME+V  DG T+GEVM RGNT+MSGY+K
Sbjct: 408 WDSLSLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYFK 467

Query: 421 NLKATCEAFSGDWYRTGDVAIRHKSGRIEMKDRAKDIVVRPNGEAVSTVAVEAVLMSHSK 480
           +++AT +AF GDW+ +GD+A+++  G IE+KDR KD+++   GE +S+V VE VL SH  
Sbjct: 468 DIEATRKAFEGDWFHSGDLAVKYPDGYIEIKDRLKDVII-SGGENISSVEVERVLCSHQA 527

Query: 481 VAEAAVIGKFEVGFGERLCGFVKLKNG-SKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPM 532
           V EAAV+ + +  +G+  CGFVKLK G   +  EEII FCR HLP +M P+ +VFGD+P 
Sbjct: 528 VLEAAVVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDIPK 587

BLAST of Tan0003265 vs. TAIR 10
Match: AT1G20560.1 (acyl activating enzyme 1 )

HSP 1 Score: 479.2 bits (1232), Expect = 4.4e-135
Identity = 250/547 (45.70%), Postives = 347/547 (63.44%), Query Frame = 0

Query: 1   MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
           ME    SPAN+ PL+P+ FL R++ VY  R S+VYG   +TW +T  RC+++ASAL    
Sbjct: 3   MEGTIKSPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASAL-SQL 62

Query: 61  HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
            +S GD+V+ +APN+P + ELHF VPMAGA++C LN + D++ +++LL+    K+IF D 
Sbjct: 63  GISTGDVVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADH 122

Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDTPPS------EFPDYNRVLEMRFGDF-TS 180
           Q+L I   +         K+P LVLIP  L    S      E  +Y  V+ M   DF   
Sbjct: 123 QFLQIAEGACEILSNKGDKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVI 182

Query: 181 KPNGELDPISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGICNTPSPVFLWTVDMFR 240
           +P  E D IS+N TSG+T   KG +YSHR AYLNSLA +  + + +  SP +LWT  MF 
Sbjct: 183 RPTDECDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVLLNEMHS--SPTYLWTNPMFH 242

Query: 241 CNGWCFIWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMI--SESSHPKP 300
           CNGWC +W + A+GG NICLR VT  AIF+++  HKVT + G  T+L MI  +  S  KP
Sbjct: 243 CNGWCLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKP 302

Query: 301 LSRRVDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFD----EETV 360
           L  +V  I   A P   ++ K+ E GF++ + YG+TE  GP  I  WKP +D    EE  
Sbjct: 303 LPGKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQA 362

Query: 361 QFE-----DLITSLEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFS 420
           + +     + +   EI VKDP++M ++ ADG T+GEV+ RGNT+M+GY KN +AT EAF 
Sbjct: 363 KMKARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFK 422

Query: 421 GDWYRTGDVAIRHKSGRIEMKDRAKDIVVRPNGEAVSTVAVEAVLMSHSKVAEAAVIGKF 480
           G W+ +GD+ ++H  G IE+KDR+KDI++   GE +S++ VE+ L +H  V EAAV+ + 
Sbjct: 423 GGWFWSGDLGVKHPDGYIELKDRSKDIII-SGGENISSIEVESTLFTHPCVLEAAVVARP 482

Query: 481 EVGFGERLCGFVKLKNGSKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAV 530
           +  +GE  C FVKLK+GSK S EE+I +CR  LP +M P  +VF DLP  STGK+QKF +
Sbjct: 483 DEYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVL 542

BLAST of Tan0003265 vs. TAIR 10
Match: AT1G66120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 409.5 bits (1051), Expect = 4.3e-114
Identity = 222/556 (39.93%), Postives = 348/556 (62.59%), Query Frame = 0

Query: 1   MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
           M++     AN  PL+P+ FLKRAS  Y  R S++YG   FTW +TY RC +LA++L+   
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLL-SL 60

Query: 61  HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
           +++  D+V+ +APN+P +YE+HF+VPM GA++ P+NT+LDA T++++L+  EPKI+F+D 
Sbjct: 61  NITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDY 120

Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDT---PPSEFPDYNRVLEMRFGDFTS---- 180
           ++ P++ + L     +  +    +++  ++D+   P S+  DY  ++  R G+ T     
Sbjct: 121 EFAPLIQEVLRLIPTYQSQPHPRIILINEIDSTTKPFSKELDYEGLI--RKGEPTPSSSA 180

Query: 181 ---KPNGELDPISINCTSGSTGLHKGAIYSHRAAYLNSLATI--FRSGICNTPSPVFLWT 240
              + + E DPIS+N TSG+T   KG + SH+ AYL++L++I  +  GI     PV+LWT
Sbjct: 181 SMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGI----FPVYLWT 240

Query: 241 VDMFRCNGWCFIWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMISESSH 300
           + MF CNGW   W++AA GG N+C+R VT   I+ ++ELH VT +    T+ + + E S 
Sbjct: 241 LPMFHCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEGSR 300

Query: 301 --PKPLSRRVDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFDE-- 360
               P S  V ++  G+ P   ++ KV + GF++ +GYG+TEA GP +   W+  +++  
Sbjct: 301 TDQSPKSSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEWNKLP 360

Query: 361 ETVQFE-------DLITSLEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKAT 420
           E  Q E         +T  ++DVK+  ++ESV  DG+T+GE++++G++LM GY KN KAT
Sbjct: 361 EHQQIELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKNPKAT 420

Query: 421 CEAFSGDWYRTGDVAIRHKSGRIEMKDRAKDIVVRPNGEAVSTVAVEAVLMSHSKVAEAA 480
            EAF   W  TGD+ + H  G +E+KDR+KDI++   GE +S++ VE VL  + +V EAA
Sbjct: 421 SEAFKHGWLNTGDIGVIHPDGYVEIKDRSKDIII-SGGENISSIEVEKVLYMYQEVLEAA 480

Query: 481 VIGKFEVGFGERLCGFVKLKNGSK---VSVEEIIKFCRAHLPEFMVPEKVV-FGDLPMNS 530
           V+      +GE  C FV LK G +    S  ++IK+CR ++P FM P+KVV F +LP NS
Sbjct: 481 VVAMPHPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQELPKNS 540

BLAST of Tan0003265 vs. TAIR 10
Match: AT1G20560.2 (acyl activating enzyme 1 )

HSP 1 Score: 402.9 bits (1034), Expect = 4.0e-112
Identity = 214/470 (45.53%), Postives = 295/470 (62.77%), Query Frame = 0

Query: 78  LYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDSQYLPIVLKSLSQNGKFN 137
           + ELHF VPMAGA++C LN + D++ +++LL+    K+IF D Q+L I   +        
Sbjct: 1   MVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQFLQIAEGACEILSNKG 60

Query: 138 IKIPALVLIPADLDTPPS------EFPDYNRVLEMRFGDF-TSKPNGELDPISINCTSGS 197
            K+P LVLIP  L    S      E  +Y  V+ M   DF   +P  E D IS+N TSG+
Sbjct: 61  DKVPILVLIPEPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIRPTDECDAISVNYTSGT 120

Query: 198 TGLHKGAIYSHRAAYLNSLATIFRSGICNTPSPVFLWTVDMFRCNGWCFIWAMAALGGCN 257
           T   KG +YSHR AYLNSLA +  + + +  SP +LWT  MF CNGWC +W + A+GG N
Sbjct: 121 TSSPKGVVYSHRGAYLNSLAAVLLNEMHS--SPTYLWTNPMFHCNGWCLLWGVTAIGGTN 180

Query: 258 ICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMI--SESSHPKPLSRRVDLIVAGALPIHE 317
           ICLR VT  AIF+++  HKVT + G  T+L MI  +  S  KPL  +V  I   A P   
Sbjct: 181 ICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPESEQKPLPGKVSFITGAAPPPAH 240

Query: 318 ILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNFD----EETVQFE-----DLITSLEID 377
           ++ K+ E GF++ + YG+TE  GP  I  WKP +D    EE  + +     + +   EI 
Sbjct: 241 VIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMKARQGVNHLGLEEIQ 300

Query: 378 VKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWYRTGDVAIRHKSGR 437
           VKDP++M ++ ADG T+GEV+ RGNT+M+GY KN +AT EAF G W+ +GD+ ++H  G 
Sbjct: 301 VKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGWFWSGDLGVKHPDGY 360

Query: 438 IEMKDRAKDIVVRPNGEAVSTVAVEAVLMSHSKVAEAAVIGKFEVGFGERLCGFVKLKNG 497
           IE+KDR+KDI++   GE +S++ VE+ L +H  V EAAV+ + +  +GE  C FVKLK+G
Sbjct: 361 IELKDRSKDIII-SGGENISSIEVESTLFTHPCVLEAAVVARPDEYWGETACAFVKLKDG 420

Query: 498 SKVSVEEIIKFCRAHLPEFMVPEKVVFGDLPMNSTGKIQKFAVREKAKAL 530
           SK S EE+I +CR  LP +M P  +VF DLP  STGK+QKF +R KAKAL
Sbjct: 421 SKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRTKAKAL 467

BLAST of Tan0003265 vs. TAIR 10
Match: AT1G76290.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 386.3 bits (991), Expect = 3.9e-107
Identity = 218/544 (40.07%), Postives = 323/544 (59.38%), Query Frame = 0

Query: 1   MESFFHSPANFAPLSPVGFLKRASAVYGGRPSVVYGGKLFTWSETYGRCLKLASALVHHF 60
           ME      AN+ PLSP+ FL+RA+ V+G R SVVYG   +TW +T  RC++LASAL    
Sbjct: 1   MEGTMRCSANYVPLSPISFLERAAVVFGSRTSVVYGDIQYTWHQTRDRCVRLASAL-SDL 60

Query: 61  HVSPGDLVAAMAPNIPELYELHFAVPMAGAIICPLNTKLDAATLSLLLQQLEPKIIFIDS 120
            +S  D+VAA+APN+P L EL+F  PMAGA++C LNT  D+  L++ L++ +PK+ F+DS
Sbjct: 61  GLSRHDVVAALAPNVPALCELYFGAPMAGAVLCVLNTTFDSQMLAMALEKTKPKVFFVDS 120

Query: 121 QYLPIVLKSLSQNGKFNIKIPALVLIPADLDTPPSEFPDYNRVLEMRFGDFTS-KPNGEL 180
           ++L +  +SLS     NI+   L++   +  T  S++  Y   L     +F   +P  E 
Sbjct: 121 EFLSVAEESLSLLS--NIEEKPLIITITENPTEQSKYEQYEDFLSTGNPNFKPIRPVDEC 180

Query: 181 DPISINCTSGSTGLHKGAIYSHRAAYLNSLATIFRSGICN--TPSPVFLWTVDMFRCNGW 240
           DPI++N TSG+T   K  +YSHR AYLN+ A     G+ N   P PV+L TV M+ C+GW
Sbjct: 181 DPIALNFTSGTTSTPKCVVYSHRGAYLNATAV----GVMNEMKPMPVYLCTVPMYHCSGW 240

Query: 241 CFIWAMAALGGCNICLRTVTVDAIFNHVELHKVTLLCGPTTLLKMISES--SHPKPLSRR 300
           C+IW + A GG  +CLR V  + IF+ +  HKVT   G   +L MI+ +  S  K     
Sbjct: 241 CYIWTVTAFGGVIVCLREVNDEVIFDSIVKHKVTNFGGSPPVLNMIANARDSVKKSFPWT 300

Query: 301 VDLIVAGALPIHEILTKVTEFGFNISYGYGMTEALGPAIIRPWKPNF----DEETVQFE- 360
           V ++  G+ P  E++ K+ + GF +   YG +E  G      W P +    +EE+++ + 
Sbjct: 301 VQVMSGGSSP-PEVMLKLKKLGFKVMMAYGCSEVYGLGTACLWMPEWETLPEEESLRLKA 360

Query: 361 ----DLITSLEIDVKDPMSMESVLADGETLGEVMLRGNTLMSGYYKNLKATCEAFSGDWY 420
               +      +DV DP +M+SV  DG+T+  + LRGNT+MSGY+K+ +AT  AF G WY
Sbjct: 361 RDGLNHFAKEAVDVLDPTTMKSVPHDGKTIRVIALRGNTVMSGYFKDKEATEAAFRGGWY 420

Query: 421 RTGDVAIRHKSGRIEMKDRAKDIVVRPNGEAVSTVAVEAVLMSHSKVAEAAVIGKFEVGF 480
            + D+ +    G I+ KDR++D V+   GE V +  +E +L SH  V +A V+G+ +   
Sbjct: 421 WSRDMGVIDPDGYIQFKDRSQD-VITCGGEIVGSKEIEGILYSHPAVYDAGVVGRPDETL 480

Query: 481 GERLCGFVKLKNGSKVSVEEIIKFCRAHLPE---FMVPEKVVFGDLPMNSTGKIQKFAVR 528
           GE +C FVKLK G++   EEII+FC+  L      M+P+ VVF D+P   TGKI+K  +R
Sbjct: 481 GESMCAFVKLKEGAEAREEEIIEFCKRKLGNKNMKMIPKTVVFSDVPKTPTGKIRKNVLR 535