Tan0003217 (gene) Snake gourd v1

Overview
NameTan0003217
Typegene
OrganismTrichosanthes anguina (Snake gourd v1)
DescriptionrRNA N-glycosidase
LocationLG06: 30900895 .. 30902581 (-)
RNA-Seq ExpressionTan0003217
SyntenyTan0003217
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
GGAAGCTAGAAAATAAAATATGAGAGTTGTAGTTGTTGTTTACATTGTTGTTGCTCTGTGTCTGTGTTTGGTGAGTGATGGCAGTGAGGCCAATCACCTTTGTTTGTCGACCTTATCATCTTATTCTGTTAATGAGTATAAAAGCATTATCCAGGGACTAAGGAAGACAACACTGTCTACAACCAGGTTGTATGGCATACCCATCTTGGAACACACAGTCTCAAATTCTCAACGTTATGGGTACGTTCATCTTGTCAACAAAAATAAGGAAAATGTCAGTTTGGCTTTAGACGTTCTTAATCTTAGAGTTGTTGCATATTCTGCAAAAAACAACTCTTATTTTTTCTCAAATGCTCCAGATTTGGCTTTTCAAATTCTCTTCCCTAAAACCAATCAAAACATTCTGCCTTTCGACAACACATTTCAATCTATTGAAAAAGCAGCTGGTACTACAAGATTGCAAACTTTTCTTGGAGCGGATCCTCTAGAATCCGCCATCACCAACTTGTTTCAATATAATCGCAACTTTGTCCCACAATCATTTCTTGTGATCATCCAAATGATCACGGAAGCCACCAAATTCAGGTTCATTGAGCAAAGTGTTACCAATAGCATAAGGGATAAAAATAACTTTACAGCTAACCGCGCAATGGTGAGCTTAGAGGACAATTGGTCGAAACTTTCAGCTCAAATCCAAGCATCAAATTCGATACAAGGAGTATTTGGAAATATCATCACGCTCTATAACTCCAACGACGAATCAATTGCAGTGGATAGTATATATTATCCAATTGTAATGGCCAACATTGCTTTGCAAGAGTATCATTGTGATCAACTCCAAAATGCAAAGATAAGGATGCCTGGGGATGATGATATATATGGTCGCGGTCGCAGTAATGGCTTTGCTTGCGATATACAAACAAGCACGACACGCATCAGTGGTCGAGAAGGATTCTGTGCGGATGTAGAAGGTGGATCTTCTAAGGATGGTACTGCCATCATTCTATATCCATGTGGACGACAAGCTAATCAAAAATGGACGTTTCAAAATGATGGGACGATTCGATCTCTAGGCAAGTGCTTGGTTTCTAATACCTCAAGGTTCGAAAACTTTCCGGTGATTTACAGTTGTGATTTGGTGAGTCGAGGTGATGTTATCTGGAAAGTGTCTATTGATGGGACAATCTTGAATCCAAGCTCTAACCTTGTGTTGACGGCAAAAGCAGCCGCAAGTAAAAAACGCTTGACAGTGGAGCCCAATACATATGCTGCTAGTCAAGGTTGGAGAGTTGCAGATTATGTGAATCCAATTGTAACTTCCATTATAGGGTTCAAGGAGATGTGTTTGGAGGCCACAAATGAAAACAGAAATATGTGGTTAGAAAATTGTGTTAAGGGTAAAGCAGAACAGTATTGGGCACTTTATAGTGATGGAACTATTAGAATAAACAACAACCGTAGCTTGTGTGTAACCTCTCCAGCTAATACCACATCGGAAGATCCCATCGCCATCAGTTCCTGTAATGGCTCTCCCAAACAACGTTGGGTTTTCGTTCCTGATGGCACAATCTTAAACCCTGAAACTGGTAAGCGTATGGATGTCTCAGGAAGTGATATTAGTCGCAAGAAAATCATTTTGTATCCTCAAAACAATAGCCCCAACCAGCAATGGTTTCCTTTCTACTAA

mRNA sequence

GGAAGCTAGAAAATAAAATATGAGAGTTGTAGTTGTTGTTTACATTGTTGTTGCTCTGTGTCTGTGTTTGGTGAGTGATGGCAGTGAGGCCAATCACCTTTGTTTGTCGACCTTATCATCTTATTCTGTTAATGAGTATAAAAGCATTATCCAGGGACTAAGGAAGACAACACTGTCTACAACCAGGTTGTATGGCATACCCATCTTGGAACACACAGTCTCAAATTCTCAACGTTATGGGTACGTTCATCTTGTCAACAAAAATAAGGAAAATGTCAGTTTGGCTTTAGACGTTCTTAATCTTAGAGTTGTTGCATATTCTGCAAAAAACAACTCTTATTTTTTCTCAAATGCTCCAGATTTGGCTTTTCAAATTCTCTTCCCTAAAACCAATCAAAACATTCTGCCTTTCGACAACACATTTCAATCTATTGAAAAAGCAGCTGGTACTACAAGATTGCAAACTTTTCTTGGAGCGGATCCTCTAGAATCCGCCATCACCAACTTGTTTCAATATAATCGCAACTTTGTCCCACAATCATTTCTTGTGATCATCCAAATGATCACGGAAGCCACCAAATTCAGGTTCATTGAGCAAAGTGTTACCAATAGCATAAGGGATAAAAATAACTTTACAGCTAACCGCGCAATGGTGAGCTTAGAGGACAATTGGTCGAAACTTTCAGCTCAAATCCAAGCATCAAATTCGATACAAGGAGTATTTGGAAATATCATCACGCTCTATAACTCCAACGACGAATCAATTGCAGTGGATAGTATATATTATCCAATTGTAATGGCCAACATTGCTTTGCAAGAGTATCATTGTGATCAACTCCAAAATGCAAAGATAAGGATGCCTGGGGATGATGATATATATGGTCGCGGTCGCAGTAATGGCTTTGCTTGCGATATACAAACAAGCACGACACGCATCAGTGGTCGAGAAGGATTCTGTGCGGATGTAGAAGGTGGATCTTCTAAGGATGGTACTGCCATCATTCTATATCCATGTGGACGACAAGCTAATCAAAAATGGACGTTTCAAAATGATGGGACGATTCGATCTCTAGGCAAGTGCTTGGTTTCTAATACCTCAAGGTTCGAAAACTTTCCGGTGATTTACAGTTGTGATTTGGTGAGTCGAGGTGATGTTATCTGGAAAGTGTCTATTGATGGGACAATCTTGAATCCAAGCTCTAACCTTGTGTTGACGGCAAAAGCAGCCGCAAGTAAAAAACGCTTGACAGTGGAGCCCAATACATATGCTGCTAGTCAAGGTTGGAGAGTTGCAGATTATGTGAATCCAATTGTAACTTCCATTATAGGGTTCAAGGAGATGTGTTTGGAGGCCACAAATGAAAACAGAAATATGTGGTTAGAAAATTGTGTTAAGGGTAAAGCAGAACAGTATTGGGCACTTTATAGTGATGGAACTATTAGAATAAACAACAACCGTAGCTTGTGTGTAACCTCTCCAGCTAATACCACATCGGAAGATCCCATCGCCATCAGTTCCTGTAATGGCTCTCCCAAACAACGTTGGGTTTTCGTTCCTGATGGCACAATCTTAAACCCTGAAACTGGTAAGCGTATGGATGTCTCAGGAAGTGATATTAGTCGCAAGAAAATCATTTTGTATCCTCAAAACAATAGCCCCAACCAGCAATGGTTTCCTTTCTACTAA

Coding sequence (CDS)

ATGAGAGTTGTAGTTGTTGTTTACATTGTTGTTGCTCTGTGTCTGTGTTTGGTGAGTGATGGCAGTGAGGCCAATCACCTTTGTTTGTCGACCTTATCATCTTATTCTGTTAATGAGTATAAAAGCATTATCCAGGGACTAAGGAAGACAACACTGTCTACAACCAGGTTGTATGGCATACCCATCTTGGAACACACAGTCTCAAATTCTCAACGTTATGGGTACGTTCATCTTGTCAACAAAAATAAGGAAAATGTCAGTTTGGCTTTAGACGTTCTTAATCTTAGAGTTGTTGCATATTCTGCAAAAAACAACTCTTATTTTTTCTCAAATGCTCCAGATTTGGCTTTTCAAATTCTCTTCCCTAAAACCAATCAAAACATTCTGCCTTTCGACAACACATTTCAATCTATTGAAAAAGCAGCTGGTACTACAAGATTGCAAACTTTTCTTGGAGCGGATCCTCTAGAATCCGCCATCACCAACTTGTTTCAATATAATCGCAACTTTGTCCCACAATCATTTCTTGTGATCATCCAAATGATCACGGAAGCCACCAAATTCAGGTTCATTGAGCAAAGTGTTACCAATAGCATAAGGGATAAAAATAACTTTACAGCTAACCGCGCAATGGTGAGCTTAGAGGACAATTGGTCGAAACTTTCAGCTCAAATCCAAGCATCAAATTCGATACAAGGAGTATTTGGAAATATCATCACGCTCTATAACTCCAACGACGAATCAATTGCAGTGGATAGTATATATTATCCAATTGTAATGGCCAACATTGCTTTGCAAGAGTATCATTGTGATCAACTCCAAAATGCAAAGATAAGGATGCCTGGGGATGATGATATATATGGTCGCGGTCGCAGTAATGGCTTTGCTTGCGATATACAAACAAGCACGACACGCATCAGTGGTCGAGAAGGATTCTGTGCGGATGTAGAAGGTGGATCTTCTAAGGATGGTACTGCCATCATTCTATATCCATGTGGACGACAAGCTAATCAAAAATGGACGTTTCAAAATGATGGGACGATTCGATCTCTAGGCAAGTGCTTGGTTTCTAATACCTCAAGGTTCGAAAACTTTCCGGTGATTTACAGTTGTGATTTGGTGAGTCGAGGTGATGTTATCTGGAAAGTGTCTATTGATGGGACAATCTTGAATCCAAGCTCTAACCTTGTGTTGACGGCAAAAGCAGCCGCAAGTAAAAAACGCTTGACAGTGGAGCCCAATACATATGCTGCTAGTCAAGGTTGGAGAGTTGCAGATTATGTGAATCCAATTGTAACTTCCATTATAGGGTTCAAGGAGATGTGTTTGGAGGCCACAAATGAAAACAGAAATATGTGGTTAGAAAATTGTGTTAAGGGTAAAGCAGAACAGTATTGGGCACTTTATAGTGATGGAACTATTAGAATAAACAACAACCGTAGCTTGTGTGTAACCTCTCCAGCTAATACCACATCGGAAGATCCCATCGCCATCAGTTCCTGTAATGGCTCTCCCAAACAACGTTGGGTTTTCGTTCCTGATGGCACAATCTTAAACCCTGAAACTGGTAAGCGTATGGATGTCTCAGGAAGTGATATTAGTCGCAAGAAAATCATTTTGTATCCTCAAAACAATAGCCCCAACCAGCAATGGTTTCCTTTCTACTAA

Protein sequence

MRVVVVVYIVVALCLCLVSDGSEANHLCLSTLSSYSVNEYKSIIQGLRKTTLSTTRLYGIPILEHTVSNSQRYGYVHLVNKNKENVSLALDVLNLRVVAYSAKNNSYFFSNAPDLAFQILFPKTNQNILPFDNTFQSIEKAAGTTRLQTFLGADPLESAITNLFQYNRNFVPQSFLVIIQMITEATKFRFIEQSVTNSIRDKNNFTANRAMVSLEDNWSKLSAQIQASNSIQGVFGNIITLYNSNDESIAVDSIYYPIVMANIALQEYHCDQLQNAKIRMPGDDDIYGRGRSNGFACDIQTSTTRISGREGFCADVEGGSSKDGTAIILYPCGRQANQKWTFQNDGTIRSLGKCLVSNTSRFENFPVIYSCDLVSRGDVIWKVSIDGTILNPSSNLVLTAKAAASKKRLTVEPNTYAASQGWRVADYVNPIVTSIIGFKEMCLEATNENRNMWLENCVKGKAEQYWALYSDGTIRINNNRSLCVTSPANTTSEDPIAISSCNGSPKQRWVFVPDGTILNPETGKRMDVSGSDISRKKIILYPQNNSPNQQWFPFY
Homology
BLAST of Tan0003217 vs. ExPASy Swiss-Prot
Match: U3KRF8 (Seed lectin (Fragments) OS=Trichosanthes anguina OX=50544 PE=1 SV=2)

HSP 1 Score: 536.6 bits (1381), Expect = 3.4e-151
Identity = 276/530 (52.08%), Postives = 366/530 (69.06%), Query Frame = 0

Query: 32  LSSYSVNEYKSIIQGLRKTTLSTT-RLYGIPILEHTVSNSQRYGYVHLVNKNKENVSLAL 91
           LS  +   YK+ I  +R+   S T RLYGIP+L+H++SNS R+  + L +   E+++LA+
Sbjct: 8   LSEANSGTYKTFIGRVREELGSETYRLYGIPVLKHSLSNSNRFYLLTLTSNQDESITLAI 67

Query: 92  DVLNLRVVAY--SAKNNSYFFSNAPDLAFQILFPKTNQNILPFDNTFQSIEKAAGTTRLQ 151
           DV ++  VAY  +  + SYFF NAP +AF  LF  T+QN+L FDNTF+S+E AAGTTR  
Sbjct: 68  DVEDMVAVAYQPAGSHESYFFLNAPQIAFHTLFTDTHQNVLNFDNTFKSLENAAGTTRQT 127

Query: 152 TFLGADPLESAITNLFQYNRNFVPQSFLVIIQMITEATKFRFIEQSVTNSIRDKNNFTAN 211
             LG DPL+ AI+NLF  +   +P SFLVIIQM+ EA+KFRFIEQSV  S +++  F  +
Sbjct: 128 IVLGVDPLDFAISNLFNADPKLLPLSFLVIIQMVLEASKFRFIEQSVAYSFKNEKTFLPD 187

Query: 212 RAMVSLEDNWSKLSAQIQASNSIQGVFGNIITLYNSNDESIAVDSIYYPIVMANIALQEY 271
            A+VSLEDNWS++S QIQAS S+QG+FG+++ LYNSN+E I VDSIYYPI++AN+ALQ Y
Sbjct: 188 LAIVSLEDNWSEISLQIQASTSLQGLFGSVVELYNSNNELIEVDSIYYPIILANVALQLY 247

Query: 272 HCDQLQNAKIRMPGDDDIYGRGRSNGFACDIQTSTTRISGREGFCADVEGGSSKDGTAII 331
           HC           GD++           C ++T TTRISGR+  C DV G  + DG+ +I
Sbjct: 248 HCQV-------STGDNE-----------CLVETRTTRISGRDALCVDVAGALTSDGSRLI 307

Query: 332 LYPCGRQANQKWTFQNDGTIRSLGKCLVSNTSRFENFPVIYSCDLVSRGDVIWKVSIDGT 391
           LYPCG+Q NQKWTF +DGT+RSLGKCL +N S+F N  VIY C  ++  D+ W VS+ GT
Sbjct: 308 LYPCGQQVNQKWTFHSDGTVRSLGKCLATNNSKFGNLVVIYDCSKLAAEDISWDVSVGGT 367

Query: 392 ILNPS-SNLVLTAKAAASKKRLTVEPNTYAASQGWRVADYVNPIVTSIIGFKEMCLEATN 451
           I+NP+  +L LT+  A     LT+E NTY+ASQGWRV +YV PI+ SI+G  +MCLEAT+
Sbjct: 368 IMNPNYEDLALTSNKATRSTNLTMEVNTYSASQGWRVGNYVQPIIGSIVGLDDMCLEATD 427

Query: 452 ENRNMWLENCVKGKAEQYWALYSDGTIRINNNRSLCVTSPANTTSE-DPIAISSCNGSPK 511
            N NMWLE CV  + EQ WALYSDGTIR+++NR LCVT+ ++T      I I +C+GS  
Sbjct: 428 GNTNMWLEECVPNQREQSWALYSDGTIRVDDNRELCVTASSSTYDNWKVITILNCDGSNN 487

Query: 512 QRWVFVPDGTILNPETGK-RMDVSGSDISRKKIILYPQNNSPNQQWFPFY 556
           QRWVF+ DG+I  P   +  MDV+ SD+  KKIIL+  +   NQQW  FY
Sbjct: 488 QRWVFLADGSISTPGNQRLAMDVARSDVDLKKIILHRPHGDLNQQWVLFY 519

BLAST of Tan0003217 vs. ExPASy Swiss-Prot
Match: P33183 (Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2)

HSP 1 Score: 412.5 bits (1059), Expect = 7.4e-114
Identity = 238/564 (42.20%), Postives = 333/564 (59.04%), Query Frame = 0

Query: 1   MRVV---VVVYIVVALCLCLVSDGSEANHLCLSTLSSYSVNEYKSIIQGLRKTTLS-TTR 60
           MRVV   ++ + +V L +C V             L       Y+  +  LRKT  + T  
Sbjct: 1   MRVVAAAMLYFYIVVLAICSVGIQGIDYPSVSFNLDGAKSATYRDFLSNLRKTVATGTYE 60

Query: 61  LYGIPIL--EHTVSNSQRYGYVHLVNKNKENVSLALDVLNLRVVAYSAKNNSYFFSNAPD 120
           + G+P+L  E  V    R+  V L N N   V+LA+DV NL VVA+S   NSYFF +A +
Sbjct: 61  VNGLPVLRRESEVQVKSRFVLVPLTNYNGNTVTLAVDVTNLYVVAFSGNANSYFFKDATE 120

Query: 121 LAFQILFPKTNQNILPFDNTFQSIEKAAGTTRLQTFLGADPLESAITNLFQYNRNFVPQS 180
           +    LF  T QN L F   + ++E AA T R    LG  PL+ AIT+L  Y+ + V +S
Sbjct: 121 VQKSNLFVGTKQNTLSFTGNYDNLETAANTRRESIELGPSPLDGAITSL--YHGDSVARS 180

Query: 181 FLVIIQMITEATKFRFIEQSVTNSIRDKNNFTANRAMVSLEDNWSKLSAQI-QASNSIQG 240
            LV+IQM++EA +FR+IEQ V  S++   +FT N  M+S+E+NWS +S +I QA N++  
Sbjct: 181 LLVVIQMVSEAARFRYIEQEVRRSLQQATSFTPNALMLSMENNWSSMSLEIQQAGNNVSP 240

Query: 241 VFGNIITLYNSNDESIAVDSIYYPIVMANIALQEYHCDQLQNAK-IRMP----GDDDIYG 300
            FG  + L N +     VD+      +  IA+  + C    N   IRMP    G+D+ Y 
Sbjct: 241 FFGT-VQLLNYDHTHRLVDNFEELYKITGIAILLFRCSSPSNDNAIRMPLDLAGEDNKY- 300

Query: 301 RGRSNGFACDIQTSTTR-ISGREGFCADVEGGSSKDGTAIILYPCGRQANQKWTFQNDGT 360
              ++G  C ++TS TR I GR+G C DV  G   DGT + L+PCG Q NQ+WTF +D T
Sbjct: 301 ---NDGETCTLRTSFTRNIVGRDGLCVDVRNGYDTDGTPLQLWPCGTQRNQRWTFDSDDT 360

Query: 361 IRSLGKCLVSNTSRFENFPVIYSCDLVSRGDVIWKVSIDGTILNPSSNLVLTAKAAASKK 420
           IRS+GKC+ +N     +  VI++C   +   + W+V IDG+I+NPSS LV+TA  AAS+ 
Sbjct: 361 IRSMGKCMTANGLNNGSNIVIFNCSTAAENAIKWEVPIDGSIINPSSGLVMTAPRAASRT 420

Query: 421 RLTVEPNTYAASQGWRVADYVNPIVTSIIGFKEMCLEATNENRNMWLENCVKGKAEQYWA 480
            L +E N YAASQGW V + V PIV SI+G+KEMCL++  EN  +W+E+C     +Q WA
Sbjct: 421 ILLLEDNIYAASQGWTVTNNVKPIVASIVGYKEMCLQSNGENNGVWMEDCEATSLQQQWA 480

Query: 481 LYSDGTIRINNNRSLCVTSPANTTSEDPIAISSCNGSPKQRWVFVPDGTILNPETGKRMD 540
           LY D TIR+N+ R LCVT+     S+D I I  C G P QRW F  DG I+NP++   MD
Sbjct: 481 LYGDRTIRVNSTRGLCVTT-NGYNSKDLIIILKCQGLPSQRWFFNSDGAIVNPKSRHVMD 540

Query: 541 VSGSDISRKKIILYPQNNSPNQQW 552
           V  S++S ++II++P   +PNQQW
Sbjct: 541 VRASNVSLREIIIFPATGNPNQQW 556

BLAST of Tan0003217 vs. ExPASy Swiss-Prot
Match: Q41358 (Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 9.4e-101
Identity = 226/576 (39.24%), Postives = 320/576 (55.56%), Query Frame = 0

Query: 1   MRVVVVVYIVVALCLCLVSDGSEANHLCLS-------TLSSYSVNEYKSIIQGLR-KTTL 60
           MR+V  +  +  L +C +       H  ++       + +    + Y+  ++ L+ K  L
Sbjct: 1   MRLVAKLLYLAVLAICGLGIHGALTHPRVTPPVYPSVSFNLTGADTYEPFLRALQEKVIL 60

Query: 61  STTRLYGIPIL--EHTVSNSQRYGYVHLVNKNKENVSLALDVLNLRVVAYSAKNNSYFFS 120
                + +P+L  E  VS+S R+  V L N + + V+LA+DV+NL VVA+S+   SYFFS
Sbjct: 61  GNHTAFDLPVLNPESQVSDSNRFVLVPLTNPSGDTVTLAIDVVNLYVVAFSSNGKSYFFS 120

Query: 121 NAPDLAFQILFPKTNQNILPFDNTFQSIEKAAGTTRLQTFLGADPLESAITNLFQY---- 180
            +  +    LF  T Q  L F   + S+E+  G  R+   LG   L+ AI++L  Y    
Sbjct: 121 GSTAVQRDNLFVDTTQEELNFTGNYTSLERQVGFGRVYIPLGPKSLDQAISSLRTYTLTA 180

Query: 181 -NRNFVPQSFLVIIQMITEATKFRFIEQSVTNSIRDKNNFTANRAMVSLEDNWSKLSAQI 240
            +   + +  LV+IQM++EA +FR+IE  +  SI D + FT +  M+S+E+NWS +S++I
Sbjct: 181 GDTKPLARGLLVVIQMVSEAARFRYIELRIRTSITDASEFTPDLLMLSMENNWSSMSSEI 240

Query: 241 QASNSIQGVFGNIITLYNSNDESIAVDSIYYPIVMANIALQEYHCDQLQNAK-------- 300
           Q +    G+F  ++ L +  + SI V +      +  IA+  Y C  + ++         
Sbjct: 241 QQAQP-GGIFAGVVQLRDERNNSIEVTNFRRLFELTYIAVLLYGCAPVTSSSYSNNAIDA 300

Query: 301 --IRMPGDDDIYGRGRSNGFACDIQTSTTRISGREGFCADVEGGSSKDGTAIILYPCGRQ 360
             I+MP       RG      C +   T RISG +G C DV  G   DG  + L PCG +
Sbjct: 301 QIIKMP-----VFRGGEYEKVCSVVEVTRRISGWDGLCVDVRYGHYIDGNPVQLRPCGNE 360

Query: 361 ANQKWTFQNDGTIRSLGKCLVSNTSRFENFPVIYSCDLVSRGDVIWKVSIDGTILNPSSN 420
            NQ WTF+ DGTIR LGKCL +++S      +IY C+ V      W VSIDGTI NP S 
Sbjct: 361 CNQLWTFRTDGTIRWLGKCLTASSS-----VMIYDCNTVPPEATKWVVSIDGTITNPHSG 420

Query: 421 LVLTAKAAASKKRLTVEPNTYAASQGWRVADYVNPIVTSIIGFKEMCLEATNENRNMWLE 480
           LVLTA  AA    L++E N +AA QGW V D V P+VT I+G+K+MCL    EN  +WLE
Sbjct: 421 LVLTAPQAAEGTALSLENNIHAARQGWTVGD-VEPLVTFIVGYKQMCLRENGENNFVWLE 480

Query: 481 NCVKGKAEQYWALYSDGTIRINNNRSLCVTSPANTTSEDPIAISSCNGSPKQRWVFVPDG 540
           +CV  + +Q WALY DGTIR+N+NRSLCVTS  +  S D I I  C GS  QRWVF  +G
Sbjct: 481 DCVLNRVQQEWALYGDGTIRVNSNRSLCVTSEDHEPS-DLIVILKCEGSGNQRWVFNTNG 540

Query: 541 TILNPETGKRMDVSGSDISRKKIILYPQNNSPNQQW 552
           TI NP     MDV+  D+S +KIILY    +PNQQW
Sbjct: 541 TISNPNAKLLMDVAQRDVSLRKIILYRPTGNPNQQW 563

BLAST of Tan0003217 vs. ExPASy Swiss-Prot
Match: O22415 (Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=1)

HSP 1 Score: 368.6 bits (945), Expect = 1.2e-100
Identity = 228/576 (39.58%), Postives = 318/576 (55.21%), Query Frame = 0

Query: 1   MRVVVVVYIVVALCLCLVSDGSEANHLCLS-------TLSSYSVNEYKSIIQGLR-KTTL 60
           MRVV  +  +V L +C +       H  ++       + +    + Y   ++ L+ K  L
Sbjct: 1   MRVVTKLLYLVVLAICGLGIHGALTHTRVTPPVYPSVSFNLTGADTYGPFLRALQEKVIL 60

Query: 61  STTRLYGIPIL--EHTVSNSQRYGYVHLVNKNKENVSLALDVLNLRVVAYSAKNNSYFFS 120
                + +P+L  E  VS+S R+  V L N + + V+LA+DV+NL VVA+S+   SYFFS
Sbjct: 61  GNHTAFDLPVLNPESQVSDSNRFVLVPLTNPSGDTVTLAIDVVNLYVVAFSSNGRSYFFS 120

Query: 121 NAPDLAFQILFPKTNQNILPFDNTFQSIEKAAGTTRLQTFLGADPLESAITNLFQYNRNF 180
            +  +    LF  T Q  L F   + S+E+  G  R+   LG   L  AI++L  Y  + 
Sbjct: 121 GSTAVQRDNLFVDTTQEELNFTGNYISLERQVGFGRVYIPLGPKSLAQAISSLRTYTLSA 180

Query: 181 -----VPQSFLVIIQMITEATKFRFIEQSVTNSIRDKNNFTANRAMVSLEDNWSKLSAQI 240
                + +  LV+IQM++EA +FR+IE  +  SI D + FT +  M+S+E+NWS +S++I
Sbjct: 181 GDTKPLARGLLVVIQMVSEAARFRYIELRIRTSITDASEFTPDLLMLSMENNWSSMSSEI 240

Query: 241 QASNSIQGVFGNIITLYNSNDESIAVDSIYYPIVMANIALQEYHC----------DQLQN 300
           Q +    G+F  ++ L +  +  I V +      +  IA+  Y C          + +  
Sbjct: 241 QQAQP-GGIFPGVVQLRDERNNPIEVTNFRRLFELTYIAVLLYGCAPVTSNSYTNNAIDA 300

Query: 301 AKIRMPGDDDIYGRGRSNGFACDIQTSTTRISGREGFCADVEGGSSKDGTAIILYPCGRQ 360
             I+MP       RG      C +   T RISG +G C DV  G   DG  + L PCG +
Sbjct: 301 QIIKMP-----VFRGGGYEKVCSVVEVTRRISGWDGLCVDVRDGHYIDGNTVQLGPCGNE 360

Query: 361 ANQKWTFQNDGTIRSLGKCLVSNTSRFENFPVIYSCDLVSRGDVIWKVSIDGTILNPSSN 420
            NQ WTF+ DGTIR LGKCL +++S      +IY C+ V      W VS DGTI NP S 
Sbjct: 361 CNQLWTFRTDGTIRWLGKCLTTSSS-----VMIYDCNTVPPEATKWVVSTDGTITNPRSG 420

Query: 421 LVLTAKAAASKKRLTVEPNTYAASQGWRVADYVNPIVTSIIGFKEMCLEATNENRNMWLE 480
           LVLTA  AA    L++E N +AA QGW V D V P+VT I+G+K+MCL    EN  +WLE
Sbjct: 421 LVLTAPQAAEGTALSLENNIHAARQGWTVGD-VEPLVTFIVGYKQMCLTENGENNFVWLE 480

Query: 481 NCVKGKAEQYWALYSDGTIRINNNRSLCVTSPANTTSEDPIAISSCNGSPKQRWVFVPDG 540
           +CV  + EQ WALY DGTIR+N+NRSLCVTS  +  S D I I  C GS  QRWVF  +G
Sbjct: 481 DCVLNRVEQEWALYGDGTIRVNSNRSLCVTSEDHEPS-DLIVILKCEGSGNQRWVFNTNG 540

Query: 541 TILNPETGKRMDVSGSDISRKKIILYPQNNSPNQQW 552
           TI NP     MDV+ S++S +KIILYP   +PNQQW
Sbjct: 541 TISNPNAKLVMDVAQSNVSLRKIILYPPTGNPNQQW 563

BLAST of Tan0003217 vs. ExPASy Swiss-Prot
Match: P93543 (Ribosome-inactivating protein SNAI' OS=Sambucus nigra OX=4202 PE=1 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 8.8e-99
Identity = 222/574 (38.68%), Postives = 329/574 (57.32%), Query Frame = 0

Query: 1   MRVVVVVYIVVALCLC-LVSDGSEANHLCLST--------LSSYSVNEYKSIIQGLR-KT 60
           M+VV  +  +V L +C L   G+   H    T        L+  + NEY+  +Q LR K 
Sbjct: 1   MKVVATILYLVVLAICGLGIHGAHPTHSAPPTVYPSVSFNLTEANSNEYRHFLQELRGKV 60

Query: 61  TLSTTRLYGIPIL--EHTVSNSQRYGYVHLVNKNKENVSLALDVLNLRVVAYSAKNNSYF 120
            L + R + +P+L  E  VS+S R+  V L N +++ V+LA+DV+   VVA++  + SYF
Sbjct: 61  ILGSHRAFDLPVLNPESKVSDSDRFVLVRLTNPSRKKVTLAIDVVTFYVVAFAQNDRSYF 120

Query: 121 FSNAPDLAFQILFPKTNQNILPFDNTFQSIEKAAGTTRLQTFLGADPLESAITNLFQY-- 180
           FS + ++  + LF  T Q  L F   + S+E   G  R+   LG   L  +I++L  Y  
Sbjct: 121 FSGSSEVQRENLFVDTTQEDLNFKGDYTSLEHQVGFGRVYIPLGPKSLAQSISSLSTYKS 180

Query: 181 ---NRNFVPQSFLVIIQMITEATKFRFIEQSVTNSIRDKNNFTANRAMVSLEDNWSKLSA 240
              +   + +S LV+IQM++EA +FR+I+  +  SI D   FT +  M+S+E+ WS +S+
Sbjct: 181 SAGDNKRLARSLLVVIQMVSEAARFRYIQLRIQASITDAKEFTPDLLMLSMENKWSSMSS 240

Query: 241 QIQASNSIQGVFGNIITLYNSNDESIAVDSIYYPIVMANIALQEYHCDQLQNAK---IRM 300
           +IQ +    G F  ++ L +  +  I V +      + ++A+  + C  +       I+M
Sbjct: 241 EIQQAQP-GGAFAQVVKLLDQRNHPIDVTNFRRLFQLTSVAVLLHGCPTVTKMPAYIIKM 300

Query: 301 PGDDDIYGRGRSNGFACDIQTSTTRISGREGFCADVEGGSSKDGTAIILYPCGRQANQKW 360
           P    ++  G        ++  T RI GR+GFCA+V+ G  KDGT + L  CG Q+NQ+W
Sbjct: 301 P----VFNGGEDEERCSVVEEVTRRIGGRDGFCAEVKNGDEKDGTPVQLSSCGEQSNQQW 360

Query: 361 TFQNDGTIRSLGKCLVSNTSRFENFPVIYSCDLVSRGDVIWKVSIDGTILNPSSNLVLTA 420
           TF  DGTI+SLGKCL +++S      +IY+C +V      W VSIDGTI NP S LVLTA
Sbjct: 361 TFSTDGTIQSLGKCLTTSSS-----VMIYNCKVVPPESTKWVVSIDGTITNPRSGLVLTA 420

Query: 421 KAAASKKRLTVEPNTYAASQGWRVADYVNPIVTSIIGFKEMCLEATNENRNMWLENC-VK 480
             AA    +++E N +AA QGW V + V P+VT I+G+++MCLE    N ++ L +C VK
Sbjct: 421 PKAAEGTLVSLEKNVHAARQGWIVGN-VEPLVTFIVGYEQMCLETNPGNNDVSLGDCSVK 480

Query: 481 --GKAEQYWALYSDGTIRINNNRSLCVTSPANTTSEDPIAISSCNGSPKQRWVFVPDGTI 540
              K +Q WALY DGTIR+NN+RSLCVTS   +++E PI I  C G   QRWVF  DGTI
Sbjct: 481 SASKVDQKWALYGDGTIRVNNDRSLCVTSEGKSSNE-PIIILKCLGWANQRWVFNTDGTI 540

Query: 541 LNPETGKRMDVSGSDISRKKIILYPQNNSPNQQW 552
            NP++   M V  +D+  +KIIL   + + NQQW
Sbjct: 541 SNPDSKLVMHVDQNDVPLRKIILSHPSGTSNQQW 562

BLAST of Tan0003217 vs. NCBI nr
Match: U3KRF8.2 (RecName: Full=Seed lectin; Short=SGSL; Contains: RecName: Full=Seed lectin Aalpha chain; Contains: RecName: Full=Seed lectin Abeta chain; Contains: RecName: Full=Seed lectin B chain [Trichosanthes anguina])

HSP 1 Score: 536.6 bits (1381), Expect = 2.6e-148
Identity = 276/530 (52.08%), Postives = 366/530 (69.06%), Query Frame = 0

Query: 32  LSSYSVNEYKSIIQGLRKTTLSTT-RLYGIPILEHTVSNSQRYGYVHLVNKNKENVSLAL 91
           LS  +   YK+ I  +R+   S T RLYGIP+L+H++SNS R+  + L +   E+++LA+
Sbjct: 8   LSEANSGTYKTFIGRVREELGSETYRLYGIPVLKHSLSNSNRFYLLTLTSNQDESITLAI 67

Query: 92  DVLNLRVVAY--SAKNNSYFFSNAPDLAFQILFPKTNQNILPFDNTFQSIEKAAGTTRLQ 151
           DV ++  VAY  +  + SYFF NAP +AF  LF  T+QN+L FDNTF+S+E AAGTTR  
Sbjct: 68  DVEDMVAVAYQPAGSHESYFFLNAPQIAFHTLFTDTHQNVLNFDNTFKSLENAAGTTRQT 127

Query: 152 TFLGADPLESAITNLFQYNRNFVPQSFLVIIQMITEATKFRFIEQSVTNSIRDKNNFTAN 211
             LG DPL+ AI+NLF  +   +P SFLVIIQM+ EA+KFRFIEQSV  S +++  F  +
Sbjct: 128 IVLGVDPLDFAISNLFNADPKLLPLSFLVIIQMVLEASKFRFIEQSVAYSFKNEKTFLPD 187

Query: 212 RAMVSLEDNWSKLSAQIQASNSIQGVFGNIITLYNSNDESIAVDSIYYPIVMANIALQEY 271
            A+VSLEDNWS++S QIQAS S+QG+FG+++ LYNSN+E I VDSIYYPI++AN+ALQ Y
Sbjct: 188 LAIVSLEDNWSEISLQIQASTSLQGLFGSVVELYNSNNELIEVDSIYYPIILANVALQLY 247

Query: 272 HCDQLQNAKIRMPGDDDIYGRGRSNGFACDIQTSTTRISGREGFCADVEGGSSKDGTAII 331
           HC           GD++           C ++T TTRISGR+  C DV G  + DG+ +I
Sbjct: 248 HCQV-------STGDNE-----------CLVETRTTRISGRDALCVDVAGALTSDGSRLI 307

Query: 332 LYPCGRQANQKWTFQNDGTIRSLGKCLVSNTSRFENFPVIYSCDLVSRGDVIWKVSIDGT 391
           LYPCG+Q NQKWTF +DGT+RSLGKCL +N S+F N  VIY C  ++  D+ W VS+ GT
Sbjct: 308 LYPCGQQVNQKWTFHSDGTVRSLGKCLATNNSKFGNLVVIYDCSKLAAEDISWDVSVGGT 367

Query: 392 ILNPS-SNLVLTAKAAASKKRLTVEPNTYAASQGWRVADYVNPIVTSIIGFKEMCLEATN 451
           I+NP+  +L LT+  A     LT+E NTY+ASQGWRV +YV PI+ SI+G  +MCLEAT+
Sbjct: 368 IMNPNYEDLALTSNKATRSTNLTMEVNTYSASQGWRVGNYVQPIIGSIVGLDDMCLEATD 427

Query: 452 ENRNMWLENCVKGKAEQYWALYSDGTIRINNNRSLCVTSPANTTSE-DPIAISSCNGSPK 511
            N NMWLE CV  + EQ WALYSDGTIR+++NR LCVT+ ++T      I I +C+GS  
Sbjct: 428 GNTNMWLEECVPNQREQSWALYSDGTIRVDDNRELCVTASSSTYDNWKVITILNCDGSNN 487

Query: 512 QRWVFVPDGTILNPETGK-RMDVSGSDISRKKIILYPQNNSPNQQWFPFY 556
           QRWVF+ DG+I  P   +  MDV+ SD+  KKIIL+  +   NQQW  FY
Sbjct: 488 QRWVFLADGSISTPGNQRLAMDVARSDVDLKKIILHRPHGDLNQQWVLFY 519

BLAST of Tan0003217 vs. NCBI nr
Match: XP_011656527.1 (seed lectin [Cucumis sativus] >KGN45978.1 hypothetical protein Csa_005239 [Cucumis sativus])

HSP 1 Score: 528.5 bits (1360), Expect = 7.0e-146
Identity = 284/550 (51.64%), Postives = 374/550 (68.00%), Query Frame = 0

Query: 8   YIVVALCLCLVSDGSEANHLCLSTLSSYSVNEYKSIIQGLR-KTTLSTTRLYGIPILEHT 67
           YI++A  L L +   + N L LS+ S   V+ YK+ I  +R + T +T +LY IPILE +
Sbjct: 7   YIIIAF-LSLATPSIDGNQLSLSS-SDLVVDGYKNFINVIRQRLTENTLKLYDIPILESS 66

Query: 68  VSNSQRYGYVHLVNKNKENVSLALDVLNLRVVAYSAKNNSYFFSNAPDLAFQILFPKTNQ 127
           +  +QR+  +++ N+N E +SLA+D +NL VV Y + NNSY F +AP  A  ++FP+T +
Sbjct: 67  LPPTQRFMNINIGNQNDETISLAIDRVNLGVVGYRSNNNSYIFLDAPKAALDVVFPETCR 126

Query: 128 NILPFDNTFQSIEKAAGTTRLQTFLGADPLESAITNLFQYNRNFVPQSFLVIIQMITEAT 187
            +L F++ ++SIEKA+GTTRLQT LG +PL SAI+NLF Y R  +P SFLVI+QM+ E +
Sbjct: 127 VVLGFNSDYESIEKASGTTRLQTLLGLEPLNSAISNLFHYRRADIPGSFLVILQMVLEGS 186

Query: 188 KFRFIEQSVTNSIRDKNNFTANRAMVSLEDNWSKLSAQIQASNSIQGVFGNIITLYNSND 247
           KF+FIEQSV  S++   NF    A+VSLEDNW KLS+QIQAS S+QG+FG  I LY+SND
Sbjct: 187 KFKFIEQSVIQSLKYGYNFKPGLAIVSLEDNWDKLSSQIQASPSLQGLFGEAIRLYDSND 246

Query: 248 ESIAVDSIYYPIVMANIALQEYHCDQLQNAKIRMPGDDDIYGRGRSNGFACDIQTSTTRI 307
           + I VDSIYY I+  NIA Q +HC+   N  IRMP D D          +C++QT T  I
Sbjct: 247 KFIDVDSIYYVIITTNIAFQLHHCNVSTNF-IRMPSDVD---------DSCNVQTRTAMI 306

Query: 308 SGREGFCADVEGGSSKDGTAIILYPCGRQANQKWTFQNDGTIRSLGKCLVSNTSRFENFP 367
           SG+ GFC D       +G  IILY C  Q NQ+WTF +D TIR   KCL   TSR   + 
Sbjct: 307 SGQNGFCVDSSRILDYNGNPIILYRCINQLNQEWTFLSDKTIRYSNKCLTFETSR---YV 366

Query: 368 VIYSC-DLVSRGDVIWKVSIDGTILNPSSNLVLTAKAAASKKRLTVEPNTYAASQGWRVA 427
           V+Y+C ++  +G++ W V+IDGTI NPSS LVLT   + ++ +L VE N +  SQGWRV 
Sbjct: 367 VLYNCSEVEEKGNIRWNVAIDGTISNPSSGLVLTTDPSTNRSQLIVEVNKFTTSQGWRVG 426

Query: 428 DYVNPIVTSIIGFKEMCLEATNENRNMWLENCVKGKAEQYWALYSDGTIRINNNRSLCVT 487
           +YV PI+ SIIG +EMCLEATN N NMWLE CVK KAEQYWA+YSDG+IR+N  R+LCV+
Sbjct: 427 NYVKPIIGSIIGMEEMCLEATNNNTNMWLEKCVKNKAEQYWAVYSDGSIRVNRKRNLCVS 486

Query: 488 SPANTTSEDPIAISSCNGSPKQRWVFVPDGTILNPETGKRMDVSGSDISRKKIILYPQNN 547
           S +N  S   + I  C G+  QRW F+ +GTILNPET K +DV GS +S K+IILYP+  
Sbjct: 487 SSSN-RSGALLIIDECKGTSNQRWNFLANGTILNPETKKVVDVYGSMVSAKRIILYPKTG 540

Query: 548 SPNQQWFPFY 556
             NQQW  FY
Sbjct: 547 LANQQWTLFY 540

BLAST of Tan0003217 vs. NCBI nr
Match: XP_038884101.1 (seed lectin-like [Benincasa hispida])

HSP 1 Score: 522.3 bits (1344), Expect = 5.0e-144
Identity = 284/550 (51.64%), Postives = 371/550 (67.45%), Query Frame = 0

Query: 8   YIVVALCLCLVS-DGSEANHLCLSTLSSYSVNEYKSIIQGLR-KTTLSTTRLYGIPILEH 67
           YI+V L L   S DG++   L LS+ S   V+ YK+ I  +R + T +T++LY IPIL++
Sbjct: 7   YIIVFLSLATRSIDGNQ--ELPLSS-SDLLVDGYKNFINTIRERLTKNTSKLYDIPILKN 66

Query: 68  TVSNSQRYGYVHLVNKNKENVSLALDVLNLRVVAYSAKNNSYFFSNAPDLAFQILFPKTN 127
           ++  ++ +  ++L N N E + LA+DV+NL VV Y + N SY F NAP  A +I+FP T 
Sbjct: 67  SLPPTEPFITINLKNANNETIVLAIDVVNLGVVGYRSNNTSYVFFNAPPEASEIVFPSTC 126

Query: 128 QNILPFDNTFQSIEKAAGTTRLQTFLGADPLESAITNLFQYNRNFVPQSFLVIIQMITEA 187
           + +L FD+ ++SIEKA+G +RLQT LG DPL SAI+NLF Y++  +PQSFLVI+QM+ E 
Sbjct: 127 RVLLRFDSDYESIEKASGISRLQTLLGTDPLNSAISNLFHYHQESLPQSFLVILQMVLEG 186

Query: 188 TKFRFIEQSVTNSIRDKNNFTANRAMVSLEDNWSKLSAQIQASNSIQGVFGNIITLYNSN 247
            KF+FIEQSV  S++   NF  + AM+SL+DNW+KLS QIQAS S+QG+FG  I  Y+SN
Sbjct: 187 VKFKFIEQSVIKSLKYGYNFKPSLAMISLQDNWAKLSLQIQASTSLQGLFGEAINFYDSN 246

Query: 248 DESIAVDSIYYPIVMANIALQEYHCDQLQNAKIRMPGDDDIYGRGRSNGFACDIQTSTTR 307
           +  I VDSIYYPI++ NIALQ Y C+   N  IRMP          ++   C IQT T+ 
Sbjct: 247 NRIIQVDSIYYPIIITNIALQLYQCNVSTNF-IRMP--------SITSDSPCYIQTQTSS 306

Query: 308 ISGREGFCADVEGGSSKDGTAIILYPCGRQANQKWTFQNDGTIRSLGKCLVSNTSRFENF 367
           ISGREGFC D   G   DG  II  PC  Q+N+KW+FQ DGTIR   KCL  +TSR   F
Sbjct: 307 ISGREGFCIDATRGIQYDGNPIISSPCEHQSNEKWSFQRDGTIRYSNKCLTFDTSR---F 366

Query: 368 PVIYSCDLVSRGDVIWKVSIDGTILNPSSNLVLTAKAAASKKRLTVEPNTYAASQGWRVA 427
            V+Y+C  V      W V+IDGTI NPSS LVLTA ++ S  +L VE N Y   QGWRV 
Sbjct: 367 VVLYNCSKVEAKSTKWNVAIDGTISNPSSGLVLTANSSTSNTQLIVEANKYTIGQGWRVG 426

Query: 428 DYVNPIVTSIIGFKEMCLEATNENRNMWLENCVKGKAEQYWALYSDGTIRINNNRSLCVT 487
           +YV PI+ SIIG ++MCLEATN N NMWLENCV  KAEQYWA+YSDG+IR+N+ R+LCVT
Sbjct: 427 NYVEPIIGSIIGLEQMCLEATNNNTNMWLENCVLNKAEQYWAVYSDGSIRVNSERNLCVT 486

Query: 488 SPANTTSEDPIAISSCNGSPKQRWVFVPDGTILNPETGKRMDVSGSDISRKKIILYPQNN 547
           S ++ +S   I I  CNG+  QRW F  DGTI N +T   +DV  + +S K+IIL+ +  
Sbjct: 487 SSSDRSSA-LIVIDECNGTANQRWNFKADGTIFNLKTKLVVDVERAMVSLKRIILFRKTG 540

Query: 548 SPNQQWFPFY 556
           +PNQ W  FY
Sbjct: 547 NPNQDWGLFY 540

BLAST of Tan0003217 vs. NCBI nr
Match: XP_038877566.1 (seed lectin-like [Benincasa hispida])

HSP 1 Score: 519.6 bits (1337), Expect = 3.3e-143
Identity = 281/553 (50.81%), Postives = 376/553 (67.99%), Query Frame = 0

Query: 5   VVVYIVVALCLCLVSDGSEANHLCLSTLSSYSVNEYKSIIQGLRKT-TLSTTRLYGIPIL 64
           +V+YIVV L L   +   E N L LS+L SY+ N+YK  IQ +R + T +T +LYGIP+L
Sbjct: 4   LVLYIVVFLSLATCT--IEGNQLSLSSLDSYA-NDYKHFIQAIRDSLTQNTYQLYGIPVL 63

Query: 65  EHTVSNSQRYGYVHLVNKNKENVSLALDVLNLRVVAYSAKNNSYFFSNAPDLAFQILFPK 124
           +  V   +R+    L N+  E ++LA+D +NL VV Y + N SY F NAP  AF+++FP 
Sbjct: 64  KKVVPPFERFTTFTLTNEIDETITLAIDKVNLGVVGYLSNNISYMFLNAPREAFRVVFPS 123

Query: 125 TNQNILPFDNTFQSIEKAAGTTRLQTFLGADPLESAITNLFQYNRNFVPQSFLVIIQMIT 184
           T + IL FD+ ++SIE AA TTR +  LG DP++ AI+ LF Y  N +P++FLVI+QM+ 
Sbjct: 124 TCRVILDFDSDYKSIEIAASTTRQEIVLGFDPMDVAISTLFHYQENSIPKAFLVILQMVI 183

Query: 185 EATKFRFIEQSVTNSIRDKNNFTANRAMVSLEDNWSKLSAQIQASNSIQGVFGNIITLYN 244
           E  KF+FIEQSV  S++ + NF  + A+VSL+DNW +LS+QIQAS+S+QG+FG +I LY+
Sbjct: 184 EGLKFKFIEQSVFMSLKYRYNFKPSLAIVSLQDNWVRLSSQIQASSSLQGLFGEVIELYD 243

Query: 245 SNDESIAVDSIYYPIVMANIALQEYHCDQLQNAKIRMPGDDDIYGRGRSNGFACDIQTST 304
           SND+ I VD+I YPI++ NIA+Q YHC+   N  I+M   +D          +C IQ+ +
Sbjct: 244 SNDKIIRVDAIDYPIILTNIAMQLYHCNVSTNI-IKMTVQND----------SCYIQSHS 303

Query: 305 TRISGREGFCADVEGGSSKDGTAIILYPCGRQANQKWTFQNDGTIRSLGKCLVSNTSRFE 364
           TRISGR G CADV+ G S DG  IILYPCG+QANQ WTFQ DGTI+SLGKCL  N +   
Sbjct: 304 TRISGRAGLCADVKNGFSNDGNPIILYPCGQQANQNWTFQRDGTIQSLGKCLTFNDT--- 363

Query: 365 NFPVIYSCDLVSRGDVIWKVSIDGTILNPSSNLVLTAKAAASKKRLTVEPNTYAASQGWR 424
           NF VIY+C  V+   + WKVSIDGTI +PSS LVLTA + A+  +L +E NT   SQ WR
Sbjct: 364 NFVVIYNCSKVAPSTIKWKVSIDGTISHPSSGLVLTANSPAANTKLKMEANTQTFSQCWR 423

Query: 425 VADYVNPIVTSIIGFKEMCLEATNENRNMWLENCVKGKAEQYWALYSDGTIRINNNRSLC 484
           V +Y+ PIV SIIGF  +CLEATN+N N+ L +CVK K +QYWALY DGTIR+N++R+LC
Sbjct: 424 VGNYIEPIVGSIIGFYGVCLEATNDNTNLKLADCVKNKRQQYWALYGDGTIRVNSSRNLC 483

Query: 485 VTSPANTTSED-PIAISSCNGSPKQRWVFVPDGTILNPETGKRMDVSGSDISRKKIILYP 544
           V+S ++       + +  CNG   QRW F  +G+I+NP+ G  MDV   D     IILYP
Sbjct: 484 VSSSSSDCIPGRVVTVLKCNGGSNQRWYFNVNGSIMNPKCGMGMDV---DTRWNDIILYP 536

Query: 545 QNNSPNQQWFPFY 556
           +     QQW+ FY
Sbjct: 544 KTGEATQQWYLFY 536

BLAST of Tan0003217 vs. NCBI nr
Match: KAG7013862.1 (hypothetical protein SDJN02_24031, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 518.8 bits (1335), Expect = 5.6e-143
Identity = 275/542 (50.74%), Postives = 366/542 (67.53%), Query Frame = 0

Query: 15  LCLVSDGSEANHLCLSTLSSYSVNEYKSIIQGLRK-TTLSTTRLYGIPILEHTVSNSQRY 74
           LC+ +   E NHLC S+ S    + YKS IQ LR+  T++T+ LY IPIL+H+V  SQR+
Sbjct: 12  LCVATPNIEGNHLCFSS-SDSDADSYKSFIQQLRQYFTINTSILYDIPILKHSVPPSQRF 71

Query: 75  GYVHLVNKNKENVSLALDVLNLRVVAYSAKNNSYFFSNAPDLAFQILFPKTNQNILPFDN 134
             ++L N   E++ +A+DVL LR V Y + + SY F +AP +AF+ +FP T + +L F++
Sbjct: 72  LTINLDNPYGEDIFVAVDVLTLRPVGYRSNDTSYVFFDAPRVAFETVFPSTCRVLLGFNS 131

Query: 135 TFQSIEKAAGTTRLQTFLGADPLESAITNLFQYNRNFVPQSFLVIIQMITEATKFRFIEQ 194
            F+SIE AA T+RL T LG  P ESA+++LF Y R+ VP SFLVIIQM+ EATKF+FIEQ
Sbjct: 132 DFESIENAAATSRLDTLLGLAPFESAVSDLFHYRRDSVPTSFLVIIQMLLEATKFKFIEQ 191

Query: 195 SVTNSIRDKNNFTANRAMVSLEDNWSKLSAQIQASNSIQGVFGNIITLYNSNDESIAVDS 254
           SV NS+++   F    A++SL+DNW+KLS QIQAS+S+QG+FG  I LY+SN   I VDS
Sbjct: 192 SVVNSLKNGVPFKPTLAILSLQDNWAKLSLQIQASSSLQGLFGEPIELYDSNHRIIQVDS 251

Query: 255 IYYPIVMANIALQEYHCDQLQNAKIRMPGDDDIYGRGRSNGFACDIQTSTTRISGREGFC 314
           IYYPI++AN+ALQ Y C+   N  IRMP            G  C +QT T RISG +GFC
Sbjct: 252 IYYPIIIANMALQLYQCNIGTNF-IRMPS---------VGGDPCYVQTRTARISGLDGFC 311

Query: 315 ADVEGGSSKDGTAIILYPCGRQANQKWTFQNDGTIRSLGKCLVSNTSRFENFPVIYSCDL 374
            + +     DG  +IL PCG +  Q+W+FQ+DG I   GKCL  N S   ++ V Y+C  
Sbjct: 312 VEAQNKGWSDGNPVILSPCGEKLTQRWSFQSDGRIGYSGKCLSFNLS---SYVVTYNCSE 371

Query: 375 VSRGDVIWKVSIDGTILNPSSNLVLTAKAAASKKRLTVEPNTYAASQGWRVADYVNPIVT 434
            + G + WKVS++G I NP+S+LVLTA A+     LT EPN     Q WRV +YV PIV 
Sbjct: 372 GAAGSIRWKVSVEGMISNPNSDLVLTANASRRSAMLTAEPNAKTLGQSWRVGNYVWPIVG 431

Query: 435 SIIGFKEMCLEATNENRNMWLENCVKGKAEQYWALYSDGTIRINNNRSLCVTSPANTTSE 494
           SIIG +E CLE+ + N  +WLE CV+ KAEQYWA+YSDG+IR+N++R+ CV S A+ +  
Sbjct: 432 SIIGLEETCLESMDNNAKVWLEKCVEKKAEQYWAVYSDGSIRVNSSRNFCV-SAASDSPG 491

Query: 495 DPIAISSCNGSPKQRWVFVPDGTILNPETGKRMDVSGSDISRKKIILYPQNNSPNQQWFP 554
             I I++CNGS KQRWVF+ D TILNP++G  M+V GS +SR KIILYP     +QQW  
Sbjct: 492 GLITITNCNGSSKQRWVFMADATILNPKSGLVMEVEGSQVSRNKIILYPIKGRSSQQWTL 538

Query: 555 FY 556
           FY
Sbjct: 552 FY 538

BLAST of Tan0003217 vs. ExPASy TrEMBL
Match: A0A0A0K979 (rRNA N-glycosidase OS=Cucumis sativus OX=3659 GN=Csa_6G040650 PE=3 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 3.4e-146
Identity = 284/550 (51.64%), Postives = 374/550 (68.00%), Query Frame = 0

Query: 8   YIVVALCLCLVSDGSEANHLCLSTLSSYSVNEYKSIIQGLR-KTTLSTTRLYGIPILEHT 67
           YI++A  L L +   + N L LS+ S   V+ YK+ I  +R + T +T +LY IPILE +
Sbjct: 7   YIIIAF-LSLATPSIDGNQLSLSS-SDLVVDGYKNFINVIRQRLTENTLKLYDIPILESS 66

Query: 68  VSNSQRYGYVHLVNKNKENVSLALDVLNLRVVAYSAKNNSYFFSNAPDLAFQILFPKTNQ 127
           +  +QR+  +++ N+N E +SLA+D +NL VV Y + NNSY F +AP  A  ++FP+T +
Sbjct: 67  LPPTQRFMNINIGNQNDETISLAIDRVNLGVVGYRSNNNSYIFLDAPKAALDVVFPETCR 126

Query: 128 NILPFDNTFQSIEKAAGTTRLQTFLGADPLESAITNLFQYNRNFVPQSFLVIIQMITEAT 187
            +L F++ ++SIEKA+GTTRLQT LG +PL SAI+NLF Y R  +P SFLVI+QM+ E +
Sbjct: 127 VVLGFNSDYESIEKASGTTRLQTLLGLEPLNSAISNLFHYRRADIPGSFLVILQMVLEGS 186

Query: 188 KFRFIEQSVTNSIRDKNNFTANRAMVSLEDNWSKLSAQIQASNSIQGVFGNIITLYNSND 247
           KF+FIEQSV  S++   NF    A+VSLEDNW KLS+QIQAS S+QG+FG  I LY+SND
Sbjct: 187 KFKFIEQSVIQSLKYGYNFKPGLAIVSLEDNWDKLSSQIQASPSLQGLFGEAIRLYDSND 246

Query: 248 ESIAVDSIYYPIVMANIALQEYHCDQLQNAKIRMPGDDDIYGRGRSNGFACDIQTSTTRI 307
           + I VDSIYY I+  NIA Q +HC+   N  IRMP D D          +C++QT T  I
Sbjct: 247 KFIDVDSIYYVIITTNIAFQLHHCNVSTNF-IRMPSDVD---------DSCNVQTRTAMI 306

Query: 308 SGREGFCADVEGGSSKDGTAIILYPCGRQANQKWTFQNDGTIRSLGKCLVSNTSRFENFP 367
           SG+ GFC D       +G  IILY C  Q NQ+WTF +D TIR   KCL   TSR   + 
Sbjct: 307 SGQNGFCVDSSRILDYNGNPIILYRCINQLNQEWTFLSDKTIRYSNKCLTFETSR---YV 366

Query: 368 VIYSC-DLVSRGDVIWKVSIDGTILNPSSNLVLTAKAAASKKRLTVEPNTYAASQGWRVA 427
           V+Y+C ++  +G++ W V+IDGTI NPSS LVLT   + ++ +L VE N +  SQGWRV 
Sbjct: 367 VLYNCSEVEEKGNIRWNVAIDGTISNPSSGLVLTTDPSTNRSQLIVEVNKFTTSQGWRVG 426

Query: 428 DYVNPIVTSIIGFKEMCLEATNENRNMWLENCVKGKAEQYWALYSDGTIRINNNRSLCVT 487
           +YV PI+ SIIG +EMCLEATN N NMWLE CVK KAEQYWA+YSDG+IR+N  R+LCV+
Sbjct: 427 NYVKPIIGSIIGMEEMCLEATNNNTNMWLEKCVKNKAEQYWAVYSDGSIRVNRKRNLCVS 486

Query: 488 SPANTTSEDPIAISSCNGSPKQRWVFVPDGTILNPETGKRMDVSGSDISRKKIILYPQNN 547
           S +N  S   + I  C G+  QRW F+ +GTILNPET K +DV GS +S K+IILYP+  
Sbjct: 487 SSSN-RSGALLIIDECKGTSNQRWNFLANGTILNPETKKVVDVYGSMVSAKRIILYPKTG 540

Query: 548 SPNQQWFPFY 556
             NQQW  FY
Sbjct: 547 LANQQWTLFY 540

BLAST of Tan0003217 vs. ExPASy TrEMBL
Match: A0A5D3D0Q4 (rRNA N-glycosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1032G00170 PE=3 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 6.0e-143
Identity = 275/552 (49.82%), Postives = 376/552 (68.12%), Query Frame = 0

Query: 6   VVYIVVALCLCLVSDGSEANHLCLSTLSSYSVNEYKSIIQGLR-KTTLSTTRLYGIPILE 65
           + + ++A  L L +   + N   LS+ S   V+ YK+ I  +R + T +T++LY IPIL+
Sbjct: 4   LTFYIIAF-LSLATRRIDGNQHSLSS-SDLLVDGYKNFINVIRQRLTENTSKLYDIPILK 63

Query: 66  HTVSNSQRYGYVHLVNKNKENVSLALDVLNLRVVAYSAKNNSYFFSNAPDLAFQILFPKT 125
            ++  +QR+  +++VN+N E +SLA+D +NL V+ Y + N SY F +AP +AF I+FPKT
Sbjct: 64  SSLPPTQRFMTINIVNQNDETISLAIDKVNLGVLGYRSNNTSYIFLDAPLVAFDIVFPKT 123

Query: 126 NQNILPFDNTFQSIEKAAGTTRLQTFLGADPLESAITNLFQYNRNFVPQSFLVIIQMITE 185
            + +L F++ ++SIEKA+GTTRLQT LG +PL SAI+NLF Y+R  +P+SFLVI+QM+ E
Sbjct: 124 CRVLLGFNSNYESIEKASGTTRLQTLLGLEPLNSAISNLFHYHRASIPESFLVILQMVLE 183

Query: 186 ATKFRFIEQSVTNSIRDKNNFTANRAMVSLEDNWSKLSAQIQASNSIQGVFGNIITLYNS 245
            +KF+FIE+SV  S++   NF    A+VSLEDNW KLS+Q+QAS S+QG+FG  I LY+S
Sbjct: 184 GSKFKFIERSVIQSLKYGYNFKPGLAIVSLEDNWEKLSSQVQASPSLQGLFGEAIKLYDS 243

Query: 246 NDESIAVDSIYYPIVMANIALQEYHCDQLQNAKIRMPGDDDIYGRGRSNGFACDIQTSTT 305
           N++ I VDSIYY I+  NIA Q +HC+   N  IRMP D          G +C++QT T 
Sbjct: 244 NNKVIEVDSIYYAIITTNIAFQLHHCNISTNF-IRMPSD---------VGDSCNVQTRTA 303

Query: 306 RISGREGFCADVEGGSSKDGTAIILYPCGRQANQKWTFQNDGTIRSLGKCLVSNTSRFEN 365
            I+G+ GFC D   G  +D  +IILY CG Q+NQ+WTF +D TIR   KCL  +TSR   
Sbjct: 304 TITGQNGFCVDASNGLHQDDNSIILYRCGYQSNQEWTFLSDKTIRYSNKCLTFDTSR--- 363

Query: 366 FPVIYSC-DLVSRGDVIWKVSIDGTILNPSSNLVLTAKAAASKKRLTVEPNTYAASQGWR 425
           F V+Y+C ++  + ++ W V+IDGTI NPSS LVLT   + +  +L VE N +  SQGWR
Sbjct: 364 FVVLYNCSEVEKKSNIRWNVAIDGTISNPSSGLVLTTNPSTNSSQLIVEVNKFTTSQGWR 423

Query: 426 VADYVNPIVTSIIGFKEMCLEATNENRNMWLENCVKGKAEQYWALYSDGTIRINNNRSLC 485
           V +YV P+  SIIG +EMCLEATN N N+WLE CVK KAEQYWA+Y DG+IR+N+ R+LC
Sbjct: 424 VGNYVEPLTGSIIGLEEMCLEATNNNTNVWLEKCVKNKAEQYWAVYGDGSIRVNSKRNLC 483

Query: 486 VTSPANTTSEDPIAISSCNGSPKQRWVFVPDGTILNPETGKRMDVSGSDISRKKIILYPQ 545
           V+S +N      I I  CNG+  QRW F+ +G ILNPET   MDV    +SRKKIILY +
Sbjct: 484 VSSSSNRILA-LIIIEECNGTSNQRWNFLANGAILNPETKMVMDVYRFMVSRKKIILYRK 539

Query: 546 NNSPNQQWFPFY 556
               NQ+W  FY
Sbjct: 544 TGERNQEWALFY 539

BLAST of Tan0003217 vs. ExPASy TrEMBL
Match: A0A1S3BDI7 (rRNA N-glycosidase OS=Cucumis melo OX=3656 GN=LOC103488711 PE=3 SV=1)

HSP 1 Score: 517.7 bits (1332), Expect = 6.0e-143
Identity = 275/552 (49.82%), Postives = 376/552 (68.12%), Query Frame = 0

Query: 6   VVYIVVALCLCLVSDGSEANHLCLSTLSSYSVNEYKSIIQGLR-KTTLSTTRLYGIPILE 65
           + + ++A  L L +   + N   LS+ S   V+ YK+ I  +R + T +T++LY IPIL+
Sbjct: 4   LTFYIIAF-LSLATRRIDGNQHSLSS-SDLLVDGYKNFINVIRQRLTENTSKLYDIPILK 63

Query: 66  HTVSNSQRYGYVHLVNKNKENVSLALDVLNLRVVAYSAKNNSYFFSNAPDLAFQILFPKT 125
            ++  +QR+  +++VN+N E +SLA+D +NL V+ Y + N SY F +AP +AF I+FPKT
Sbjct: 64  SSLPPTQRFMTINIVNQNDETISLAIDKVNLGVLGYRSNNTSYIFLDAPLVAFDIVFPKT 123

Query: 126 NQNILPFDNTFQSIEKAAGTTRLQTFLGADPLESAITNLFQYNRNFVPQSFLVIIQMITE 185
            + +L F++ ++SIEKA+GTTRLQT LG +PL SAI+NLF Y+R  +P+SFLVI+QM+ E
Sbjct: 124 CRVLLGFNSNYESIEKASGTTRLQTLLGLEPLNSAISNLFHYHRASIPESFLVILQMVLE 183

Query: 186 ATKFRFIEQSVTNSIRDKNNFTANRAMVSLEDNWSKLSAQIQASNSIQGVFGNIITLYNS 245
            +KF+FIE+SV  S++   NF    A+VSLEDNW KLS+Q+QAS S+QG+FG  I LY+S
Sbjct: 184 GSKFKFIERSVIQSLKYGYNFKPGLAIVSLEDNWEKLSSQVQASPSLQGLFGEAIKLYDS 243

Query: 246 NDESIAVDSIYYPIVMANIALQEYHCDQLQNAKIRMPGDDDIYGRGRSNGFACDIQTSTT 305
           N++ I VDSIYY I+  NIA Q +HC+   N  IRMP D          G +C++QT T 
Sbjct: 244 NNKVIEVDSIYYAIITTNIAFQLHHCNISTNF-IRMPSD---------VGDSCNVQTRTA 303

Query: 306 RISGREGFCADVEGGSSKDGTAIILYPCGRQANQKWTFQNDGTIRSLGKCLVSNTSRFEN 365
            I+G+ GFC D   G  +D  +IILY CG Q+NQ+WTF +D TIR   KCL  +TSR   
Sbjct: 304 TITGQNGFCVDASNGLHQDDNSIILYRCGYQSNQEWTFLSDKTIRYSNKCLTFDTSR--- 363

Query: 366 FPVIYSC-DLVSRGDVIWKVSIDGTILNPSSNLVLTAKAAASKKRLTVEPNTYAASQGWR 425
           F V+Y+C ++  + ++ W V+IDGTI NPSS LVLT   + +  +L VE N +  SQGWR
Sbjct: 364 FVVLYNCSEVEKKSNIRWNVAIDGTISNPSSGLVLTTNPSTNSSQLIVEVNKFTTSQGWR 423

Query: 426 VADYVNPIVTSIIGFKEMCLEATNENRNMWLENCVKGKAEQYWALYSDGTIRINNNRSLC 485
           V +YV P+  SIIG +EMCLEATN N N+WLE CVK KAEQYWA+Y DG+IR+N+ R+LC
Sbjct: 424 VGNYVEPLTGSIIGLEEMCLEATNNNTNVWLEKCVKNKAEQYWAVYGDGSIRVNSKRNLC 483

Query: 486 VTSPANTTSEDPIAISSCNGSPKQRWVFVPDGTILNPETGKRMDVSGSDISRKKIILYPQ 545
           V+S +N      I I  CNG+  QRW F+ +G ILNPET   MDV    +SRKKIILY +
Sbjct: 484 VSSSSNRILA-LIIIEECNGTSNQRWNFLANGAILNPETKMVMDVYRFMVSRKKIILYRK 539

Query: 546 NNSPNQQWFPFY 556
               NQ+W  FY
Sbjct: 544 TGERNQEWALFY 539

BLAST of Tan0003217 vs. ExPASy TrEMBL
Match: A0A0A0KN71 (rRNA N-glycosidase OS=Cucumis sativus OX=3659 GN=Csa_5G428440 PE=3 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 2.1e-140
Identity = 261/522 (50.00%), Postives = 357/522 (68.39%), Query Frame = 0

Query: 37  VNEYKSIIQGLR-KTTLSTTRLYGIPILEHTVSNSQRYGYVHLVNKNKENVSLALDVLNL 96
           V+ Y+S IQ +R K    T +LYGIP+L H++SNS R+  +   N+  + ++ A+D  ++
Sbjct: 24  VDVYQSYIQTIRQKFGSKTHKLYGIPVLHHSLSNSDRFHLIDAGNEPGDTITFAVDAQDM 83

Query: 97  RVVAYSA-KNNSYFFSNAPDLAFQILFPKTNQNILPFDNTFQSIEKAAGTTRLQTFLGAD 156
            VVAY A  N+SYFFSNAP  AF ILFPKTNQN+L FDN+F+SIE AA TTR  T LG  
Sbjct: 84  SVVAYLAGDNDSYFFSNAPKFAFDILFPKTNQNLLNFDNSFKSIEIAANTTREATPLGLK 143

Query: 157 PLESAITNLFQYNRNFVPQSFLVIIQMITEATKFRFIEQSVTNSIRDKNNFTANRAMVSL 216
           P  +AI NLF Y+    P SFL++ QM+ E+ KF+FIEQ + NSI +   FT + AM+SL
Sbjct: 144 PSNAAIANLFHYDPVLAPVSFLIVFQMVFESAKFKFIEQRIVNSITNGEAFTPDLAMLSL 203

Query: 217 EDNWSKLSAQIQASNSIQGVFGNIITLYNSNDESIAVDSIYYPIVMANIALQEYHCDQLQ 276
           EDNWS+LS +IQASNS+QG+FG+ +TLYNS +E++ VDSIYYP++++N+ALQ YHC+   
Sbjct: 204 EDNWSELSLEIQASNSLQGLFGSSVTLYNSKNEAVEVDSIYYPLILSNLALQLYHCNIQD 263

Query: 277 NAKIRMPGDDDIYGRGRSNGFACDIQTSTTRISGREGFCADVEGGSSKDGTAIILYPCGR 336
             K+      D           C ++  T RISG++G CADV    ++DG+ +I  PCG+
Sbjct: 264 YIKMPTATVAD------HQNPRCYVKERTVRISGQDGLCADV----ARDGSHVISSPCGQ 323

Query: 337 QANQKWTFQNDGTIRSLGKCLVSNTSRFENFPVIYSCDLVSRGDVIWKVSIDGTILNPSS 396
           QANQ+WTF  D TIRS  KCL+ N S+     VI +C+ VS+ D  W VSI GTI+NP+ 
Sbjct: 324 QANQQWTFHRDHTIRSSDKCLIPNKSKANPLAVIQNCNKVSQEDATWDVSISGTIMNPAY 383

Query: 397 NLVLTAKAAASKKRLTVEPNTYAASQGWRVADYVNPIVTSIIGFKEMCLEATNENRNMWL 456
           +LVLT+K   +   L+++ N Y  +QGWRV +YV PI+ SIIG K+MCLEAT EN N+WL
Sbjct: 384 DLVLTSKNGINDNSLSMKKNKYCGNQGWRVGNYVEPIIASIIGVKQMCLEATEENTNIWL 443

Query: 457 ENCVKGKAEQYWALYSDGTIRINNNRSLCVTSPANTTSEDPIAISSCNGSPKQRWVFVPD 516
           E CVK K EQ WA++SDG+IR+NN+ SLC+T+ ++  S+  I I+ CNG   QRWV   D
Sbjct: 444 EECVKNKIEQSWAVFSDGSIRVNNDHSLCMTA-SSIESKQRIVIAKCNGLASQRWVLKAD 503

Query: 517 GTILNPE-TGKRMDVSGSDISRKKIILYPQNNSPNQQWFPFY 556
           GTI  P+  G  MDV+ S++  K+I+LYP+++  +Q W   Y
Sbjct: 504 GTISTPKYEGLVMDVAQSNVDLKEIVLYPRSDLVSQHWVALY 534

BLAST of Tan0003217 vs. ExPASy TrEMBL
Match: A0A5A7V3Q8 (rRNA N-glycosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold387G00010 PE=3 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 8.1e-140
Identity = 262/522 (50.19%), Postives = 353/522 (67.62%), Query Frame = 0

Query: 37  VNEYKSIIQGLR-KTTLSTTRLYGIPILEHTVSNSQRYGYVHLVNKNKENVSLALDVLNL 96
           V+ Y+S IQ +R K    T +LYGIP+L+H++SNS R+  +   N+  + ++LA+D  ++
Sbjct: 24  VDVYQSYIQTIRQKFGSKTHKLYGIPVLQHSLSNSDRFHLIDTGNEPGDTITLAVDAQDM 83

Query: 97  RVVAYSAKN-NSYFFSNAPDLAFQILFPKTNQNILPFDNTFQSIEKAAGTTRLQTFLGAD 156
            VVAY A N +SYFFSNAP  AF ILFP TNQN+L FD++F+SIEKAA TTR  T LG  
Sbjct: 84  SVVAYLAGNDDSYFFSNAPKFAFDILFPNTNQNLLNFDSSFKSIEKAANTTRQATPLGFK 143

Query: 157 PLESAITNLFQYNRNFVPQSFLVIIQMITEATKFRFIEQSVTNSIRDKNNFTANRAMVSL 216
              +AI NLF Y+    P SFL++ QMI E+ KF FIEQ + NSI+    FT + AM+SL
Sbjct: 144 ESNAAIANLFHYDSVLAPVSFLIVFQMIFESAKFNFIEQRIVNSIKYGEAFTPDLAMLSL 203

Query: 217 EDNWSKLSAQIQASNSIQGVFGNIITLYNSNDESIAVDSIYYPIVMANIALQEYHCDQLQ 276
           EDNWS+LS QIQASNS+QG+FG+ +TLYNS +E+I VDSIYYP++++N+ALQ YHC+   
Sbjct: 204 EDNWSELSLQIQASNSLQGLFGSSVTLYNSKNEAIEVDSIYYPLILSNLALQLYHCNIQD 263

Query: 277 NAKIRMPGDDDIYGRGRSNGFACDIQTSTTRISGREGFCADVEGGSSKDGTAIILYPCGR 336
             K+      D           C ++  T RISGR+G C DV    ++DG+ +I  PCG+
Sbjct: 264 YIKMPTATVTD------HQNPRCYVKERTVRISGRDGLCVDV----ARDGSQVISSPCGQ 323

Query: 337 QANQKWTFQNDGTIRSLGKCLVSNTSRFENFPVIYSCDLVSRGDVIWKVSIDGTILNPSS 396
           QANQ+WTF  D TIRS  KCL+   S+   F VI  C+ VS+ D  W VSI GTI+NP+ 
Sbjct: 324 QANQRWTFNRDHTIRSSDKCLIPTKSKANPFAVILDCNKVSQQDATWDVSISGTIMNPAY 383

Query: 397 NLVLTAKAAASKKRLTVEPNTYAASQGWRVADYVNPIVTSIIGFKEMCLEATNENRNMWL 456
           +LVLT+    +   L+++ N Y  +QGWRV ++V P++ SIIG K+MCLEAT EN N+WL
Sbjct: 384 DLVLTSNNGINDNSLSMKKNKYCGNQGWRVGNFVEPLIVSIIGVKQMCLEATEENTNIWL 443

Query: 457 ENCVKGKAEQYWALYSDGTIRINNNRSLCVTSPANTTSEDPIAISSCNGSPKQRWVFVPD 516
           E CVK K EQ WA+YSDG+IR+NN+ SLCVT+ ++   +  I I  CNG   QRWV   D
Sbjct: 444 EECVKNKIEQSWAVYSDGSIRVNNDHSLCVTA-SSIEPKQRIVIGKCNGLASQRWVLNAD 503

Query: 517 GTILNPET-GKRMDVSGSDISRKKIILYPQNNSPNQQWFPFY 556
           GTI  P+  G  MDV+ S++  K+I+LYP+++  +QQW   Y
Sbjct: 504 GTISTPKNEGLVMDVAQSNVDLKEIVLYPRSDLVSQQWVALY 534

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
U3KRF83.4e-15152.08Seed lectin (Fragments) OS=Trichosanthes anguina OX=50544 PE=1 SV=2[more]
P331837.4e-11442.20Nigrin b OS=Sambucus nigra OX=4202 PE=1 SV=2[more]
Q413589.4e-10139.24Ribosome-inactivating protein SNAI OS=Sambucus nigra OX=4202 GN=SNA-I PE=1 SV=1[more]
O224151.2e-10039.58Ribosome-inactivating protein SNAIf OS=Sambucus nigra OX=4202 GN=SNA-If PE=1 SV=... [more]
P935438.8e-9938.68Ribosome-inactivating protein SNAI' OS=Sambucus nigra OX=4202 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
U3KRF8.22.6e-14852.08RecName: Full=Seed lectin; Short=SGSL; Contains: RecName: Full=Seed lectin Aalph... [more]
XP_011656527.17.0e-14651.64seed lectin [Cucumis sativus] >KGN45978.1 hypothetical protein Csa_005239 [Cucum... [more]
XP_038884101.15.0e-14451.64seed lectin-like [Benincasa hispida][more]
XP_038877566.13.3e-14350.81seed lectin-like [Benincasa hispida][more]
KAG7013862.15.6e-14350.74hypothetical protein SDJN02_24031, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
A0A0A0K9793.4e-14651.64rRNA N-glycosidase OS=Cucumis sativus OX=3659 GN=Csa_6G040650 PE=3 SV=1[more]
A0A5D3D0Q46.0e-14349.82rRNA N-glycosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1032G... [more]
A0A1S3BDI76.0e-14349.82rRNA N-glycosidase OS=Cucumis melo OX=3656 GN=LOC103488711 PE=3 SV=1[more]
A0A0A0KN712.1e-14050.00rRNA N-glycosidase OS=Cucumis sativus OX=3659 GN=Csa_5G428440 PE=3 SV=1[more]
A0A5A7V3Q88.1e-14050.19rRNA N-glycosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold387G0... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Snake gourd (anguina) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR017989Ribosome-inactivating protein type 1/2PRINTSPR00396SHIGARICINcoord: 85..100
score: 45.89
coord: 209..226
score: 45.76
coord: 175..195
score: 48.87
coord: 38..51
score: 27.63
coord: 146..160
score: 42.11
IPR000772Ricin B, lectin domainSMARTSM00458ricin_3coord: 301..425
e-value: 8.0E-22
score: 88.5
coord: 429..554
e-value: 1.5E-28
score: 110.9
IPR000772Ricin B, lectin domainPFAMPF00652Ricin_B_lectincoord: 304..422
e-value: 9.8E-20
score: 71.1
coord: 439..551
e-value: 3.0E-24
score: 85.7
IPR000772Ricin B, lectin domainPROSITEPS50231RICIN_B_LECTINcoord: 300..425
score: 28.445999
IPR000772Ricin B, lectin domainPROSITEPS50231RICIN_B_LECTINcoord: 428..554
score: 33.367496
IPR000772Ricin B, lectin domainCDDcd00161RICINcoord: 435..551
e-value: 5.8116E-25
score: 97.9614
IPR000772Ricin B, lectin domainCDDcd00161RICINcoord: 305..422
e-value: 7.08502E-20
score: 83.709
IPR016139Ribosome-inactivating protein, subdomain 2GENE3D4.10.470.10Ricin (A Subunit), domain 2coord: 189..272
e-value: 2.1E-24
score: 87.2
IPR001574Ribosome-inactivating proteinPFAMPF00161RIPcoord: 38..226
e-value: 4.3E-27
score: 95.0
IPR001574Ribosome-inactivating proteinPANTHERPTHR33453FAMILY NOT NAMEDcoord: 410..539
IPR001574Ribosome-inactivating proteinPANTHERPTHR33453FAMILY NOT NAMEDcoord: 35..372
NoneNo IPR availableGENE3D2.80.10.50coord: 430..555
e-value: 9.8E-44
score: 150.0
NoneNo IPR availableGENE3D2.80.10.50coord: 295..429
e-value: 8.1E-33
score: 115.5
NoneNo IPR availablePANTHERPTHR33453:SF14RRNA N-GLYCOSIDASEcoord: 410..539
coord: 35..372
IPR016138Ribosome-inactivating protein, subdomain 1GENE3D3.40.420.10Ricin (A subunit), domain 1coord: 30..187
e-value: 1.3E-37
score: 131.1
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 430..552
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 297..424
IPR036041Ribosome-inactivating protein superfamilySUPERFAMILY56371Ribosome inactivating proteins (RIP)coord: 32..269

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Tan0003217.1Tan0003217.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0017148 negative regulation of translation
molecular_function GO:0030598 rRNA N-glycosylase activity